Cosmetic plastic material as well as preparation method and application thereof

Abstract

The invention provides a plastic and aesthetic material as well as a preparation method and application thereof, and the plastic and aesthetic material comprises the following components in percentage by weight: 20-40% of hyaluronic acid, 20-40% of poly-L-lactic acid and 30-50% of phospholipid-collagen nanoparticles. The preparation method of the phospholipid-collagen nanoparticles comprises the following steps: adding a collagen nano solution and a hydroxypropyl methyl cellulose aqueous solution into a lecithin emulsion under the ultrasonic action, and drying to obtain the phospholipid-collagen nanoparticles, the collagen nano liquid is obtained by stirring a collagen aqueous solution under an acidic condition and then crushing the collagen aqueous solution. The hyaluronic acid, the poly-L-lactic acid and the phospholipid-collagen nanoparticles are selected and combined, the prepared cosmetic and plastic material is scientific in proportion, the dosage of the hyaluronic acid is reduced, and the added poly-L-lactic acid component has the effects of easily stimulating collagen regeneration and skin tightening by combining the percutaneous high permeability of the phospholipid-collagen nanoparticles; the instant effect of hyaluronic acid and the long-term effect of poly-L-lactic acid are utilized, and the lasting effects of resisting aging, removing wrinkles and making the face young are achieved.

Description

Technical Field

[0001] This invention relates to the field of plastic surgery materials, and in particular to a cosmetic plastic surgery material, its preparation method, and its application. Background Technology

[0002] While existing products and materials such as hyaluronic acid and botulinum toxin can achieve certain effects, their traditional application methods are often inefficient and lack standardization. This can lead to adverse effects such as excessive facial swelling, stiffness, and unnatural appearance, and may even cause damage to the treated area. For example, while hyaluronic acid alone can effectively address tissue depressions, insufficient injection volume limits its effectiveness, while excessive injection volume can cause local swelling and swelling, and may even lead to deformities and other complications during displacement. Summary of the Invention

[0003] Therefore, the purpose of this invention is to propose a cosmetic surgery material and its preparation method, which obtains a material for cosmetic surgery through scientific compounding, effectively maximizing the advantages and minimizing the disadvantages, avoiding the risks of side effects from single products, and reducing the injection volume of a single product.

[0004] The technical solution of this invention is implemented as follows:

[0005] A cosmetic surgery material, by weight percentage, comprises the following components: 20%–40% hyaluronic acid, 20%–40% poly-L-lactic acid, and 30%–50% phospholipid-collagen nanoparticles;

[0006] The preparation method of the phospholipid-collagen nanoparticles includes adding collagen nano liquid and hydroxypropyl methylcellulose aqueous solution to lecithin emulsion under ultrasonic action, and drying to obtain the product;

[0007] The collagen nanofluid is obtained by stirring an aqueous collagen solution under acidic conditions and then breaking it down.

[0008] This invention uses a compound of hyaluronic acid, poly-L-lactic acid, and phospholipid-collagen nanoparticles to reduce the amount of hyaluronic acid used. Combined with the high transdermal permeability of phospholipid-collagen nanoparticles, the added poly-L-lactic acid can easily stimulate collagen regeneration and skin tightening, achieving the immediate effect of hyaluronic acid and the long-term effect of poly-L-lactic acid, resulting in a lasting anti-aging, wrinkle-reducing, and youthful facial appearance.

[0009] A further formulation comprises, by weight percentage, the following components: 25%–40% hyaluronic acid, 25%–40% poly-L-lactic acid, and 30%–45% phospholipid-collagen nanoparticles.

[0010] A further formulation comprises, by weight percentage, the following components: 25%–35% hyaluronic acid, 30%–40% poly-L-lactic acid, and 30%–40% phospholipid-collagen nanoparticles.

[0011] A further option is that, by weight percentage, the cosmetic material comprises: 30% hyaluronic acid, 30% poly-L-lactic acid, and 40% phospholipid-collagen nanoparticles;

[0012] Alternatively, by weight percentage, 30% hyaluronic acid, 35% poly-L-lactic acid, and 35% phospholipid-collagen nanoparticles;

[0013] Alternatively, by weight percentage, it contains 30% hyaluronic acid, 40% poly-L-lactic acid, and 30% phospholipid-collagen nanoparticles.

[0014] A further embodiment is that the preparation method of the collagen nanofluid includes: adjusting the pH of the collagen aqueous solution to 5.5-6.0 with hydrochloric acid, stirring for the first time, placing it at 4-6℃ for a second stirring, and ultrasonically breaking it down to obtain the collagen nanofluid.

[0015] A further proposed solution is that the first stirring time is 2-3 hours, and the second stirring time is 4-5 hours.

[0016] The ultrasonic fragmentation process takes 2–4 minutes and the ultrasonic power is 400–600 W.

[0017] The mass concentration of the collagen aqueous solution is 18–22 g / L.

[0018] A further embodiment is that the preparation method of the phospholipid-collagen nanoparticles includes: sonicating a lecithin emulsion, adding collagen nanoparticles and an aqueous solution of hydroxypropyl methylcellulose while sonicating, mixing, and then freeze-drying to obtain phospholipid-collagen nanoparticles;

[0019] The volume ratio of the collagen nanofluid, the hydroxypropyl methylcellulose aqueous solution, and the lecithin emulsion is 9–11:9–11:4–6.

[0020] The ultrasound duration is 10–20 minutes, and the ultrasound power is 300–400 W.

[0021] The lecithin emulsion has a mass concentration of 8–12 g / L, and the hydroxypropyl methylcellulose aqueous solution has a mass concentration of 18–22 g / L.

[0022] In one embodiment of the present invention, a preferred method for preparing phospholipid-collagen nanoparticles is as follows:

[0023] (1) Hydroxypropyl methylcellulose was prepared into a 20 g / L HPMC aqueous solution using deionized water; lecithin was added to deionized water to prepare a 10 g / L lecithin emulsion; collagen was prepared into a 20 g / L collagen aqueous solution using deionized water.

[0024] (2) Take collagen aqueous solution, adjust the pH value to 5.5-6.0 with 1M hydrochloric acid solution, stir for 2 hours, continue stirring at 4-6℃ for 4 hours, and use a cell disruptor with 500W ultrasonic power to sonicate for 2 minutes to obtain collagen nano liquid.

[0025] (3) Take 25 mL of lecithin emulsion, sonicate at 300 W for 10 min, and slowly add 50 mL of HPMC aqueous solution and 50 mL of collagen nano liquid while sonicating. After mixing, freeze dry to obtain phospholipid-collagen nanoparticles.

[0026] On the one hand, the present invention also provides a method for preparing cosmetic and plastic surgery materials, wherein hyaluronic acid, poly-L-lactic acid and phospholipid-collagen nanoparticles are mixed according to a formula to obtain cosmetic and plastic surgery materials.

[0027] On the other hand, the present invention also provides a cosmetic material including its application in anti-aging, wrinkle removal, and skin tightening.

[0028] A further option is that the cosmetic material includes its use in enhancing type IV collagen expression.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0030] This invention uses a combination of hyaluronic acid, poly-L-lactic acid, and phospholipid-collagen nanoparticles to create a cosmetic material. The scientifically formulated ratio reduces the amount of hyaluronic acid used. Combined with the high transdermal permeability of phospholipid-collagen nanoparticles, the added poly-L-lactic acid easily stimulates collagen regeneration and skin tightening, achieving the immediate effects of hyaluronic acid and the long-term effects of poly-L-lactic acid, resulting in a lasting anti-aging, wrinkle-reducing, and youthful facial appearance.

[0031] The cosmetic and plastic surgery material provided by this invention has excellent anti-aging effects, effectively promotes the expression of type IV collagen, helps to make the skin plump and full, and has a long-lasting effect after use. It has good effects on improving skin elasticity, wrinkles and hydration, and reduces problems such as swelling, stiffness and swelling after traditional medical aesthetic procedures. It will not endanger health and has more significant therapeutic effects on facial depressions, wrinkles, sagging and drooping. Attached Figure Description

[0032] Figure 1 The images show the patient before and after the procedure, with the left side showing the preoperative image and the right side showing the image two months after the procedure. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0034] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0035] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0036] The cosmetic and plastic surgery material prepared by this invention does not produce toxicity to fibroblasts, demonstrating good cell safety. In cell viability experiments, the cell viability of fibroblasts treated with 50–500 μg / mL of the cosmetic and plastic surgery material for 24 hours was higher than 85%.

[0037] Example 1

[0038] A cosmetic surgery material, with the following formula:

[0039] Table 1. Cosmetic and plastic surgery materials with different formulations

[0040] Formula 1 Formula 2 Formula 3 Formula 4 Formula 5 Formula 6 Hyaluronic acid 30 30 30 30 40 30 Poly-L-lactic acid 25 30 35 40 30 30 Phospholipid-collagen nanoparticles 45 40 35 30 - - collagen - - - - 30 40 Botox - - - - 10 -

[0041] Note: "-" indicates no addition.

[0042] Preparation method of phospholipid-collagen nanoparticles in formulations 1-4:

[0043] (1) Hydroxypropyl methylcellulose was prepared into a 20 g / L HPMC aqueous solution using deionized water; lecithin was added to deionized water to prepare a 10 g / L lecithin emulsion; collagen was prepared into a 20 g / L collagen aqueous solution using deionized water.

[0044] (2) Take collagen aqueous solution, adjust the pH value to 5.5-6.0 with 1M hydrochloric acid solution, stir for 2 hours, continue stirring at 4-6℃ for 4 hours, and use a cell disruptor with 500W ultrasonic power to sonicate for 2 minutes to obtain collagen nano liquid.

[0045] (3) Take 25 mL of lecithin emulsion, sonicate at 300 W for 10 min, and slowly add 50 mL of HPMC aqueous solution and 50 mL of collagen nano liquid while sonicating. After mixing, freeze dry to obtain phospholipid-collagen nanoparticles.

[0046] Preparation methods of cosmetic and plastic surgery materials in formulas 1-4:

[0047] According to the formula, hyaluronic acid, poly-L-lactic acid and phospholipid-collagen nanoparticles are mixed to obtain cosmetic and plastic surgery materials.

[0048] Preparation method of cosmetic and plastic surgery materials in Formula 5:

[0049] According to the formula, hyaluronic acid, poly-L-lactic acid, collagen and botulinum toxin are mixed to obtain cosmetic surgery materials.

[0050] Preparation method of cosmetic and plastic surgery materials in Formula 6:

[0051] Hyaluronic acid, poly-L-lactic acid, and collagen are mixed according to the formula to obtain cosmetic and plastic surgery materials.

[0052] Example 2

[0053] The functions of a 3D skin model are as follows:

[0054] A 3D skin anti-aging model was constructed based on the 3D full-thickness skin model FulKutis. The model and 2 mL of model culture medium were added to 6-well plates, and the samples were randomly divided into a control group, a model group, and a cosmetic group. The control group received no treatment, while the cosmetic group received 300 μg / mL of drug on the surface of the sample (20 μL). After drug administration, the samples were incubated. Both the model and cosmetic groups were simultaneously irradiated with ultraviolet light at a combined energy of 4000 mJ / cm². 2 The drug and irradiation were administered once daily for 4 consecutive days. The model was then incubated and cultured, and finally, residual samples were washed with PBS solution. After washing, the 3D skin anti-aging model was fixed with paraformaldehyde, prepared into sections, and immunofluorescence was used to detect type IV collagen (CoL-IV). The relative content was calculated using image processing.

[0055] Table 2. Expression of Type IV Collagen in a 3D Skin Anti-aging Model Based on Different Formulas of Cosmetic Plastic Surgery Materials

[0056] Model Group Formula 1 Formula 2 Formula 3 Formula 4 Formula 5 Formula 6 CoL-IV Relative IOD 0.236 0.875 1.223 1.075 0.938 0.554 0.682

[0057] Collagen molecules consist of three α-chains forming a unique triple helix structure, which endows collagen with high strength and stability. This structure provides essential structural support for tissues and organs, thus maintaining skin elasticity and resilience and preventing wrinkles. Type IV collagen is a fibrous protein mainly found in connective tissue, playing a role in supporting cell structure and maintaining tissue tension. Ultraviolet radiation reduces type IV collagen. When type IV collagen is abnormal, it may affect skin elasticity and resilience, leading to problems such as sagging skin and increased wrinkles. In addition, it may also cause symptoms such as joint stiffness and muscle weakness.

[0058] Table 2 shows that, compared with the model group, formulations 1-6 promoted the synthesis of type IV collagen to varying degrees. Specifically, the combination of hyaluronic acid, poly-L-lactic acid, and phospholipid-collagen nanoparticles demonstrated a good anti-aging effect in the 3D skin anti-aging model, effectively promoting the expression of type IV collagen and making the skin plump and full. Compared with formulation 6, the prepared phospholipid-collagen nanoparticles improved transdermal permeability and increased the bioavailability of collagen. The phospholipid-collagen nanoparticles prepared by HPMC, lecithin, and collagen nanofluid further enhanced the anti-aging and wrinkle-reducing effects of the combination of hyaluronic acid, poly-L-lactic acid, and phospholipid-collagen nanoparticles, retaining skin moisture, effectively improving skin condition, increasing skin elasticity, and reducing skin wrinkles, achieving a youthful and juvenile facial appearance.

[0059] Example 3

[0060] In this embodiment, the amount of lecithin emulsion is adjusted as follows:

[0061] A cosmetic surgery material, with the following formula: 30% hyaluronic acid, 30% poly-L-lactic acid, and 40% phospholipid-collagen nanoparticles;

[0062] The preparation method of phospholipid-collagen nanoparticles is as follows:

[0063] (1) Hydroxypropyl methylcellulose was prepared into a 20 g / L HPMC aqueous solution using deionized water; lecithin was added to deionized water to prepare a 10 g / L lecithin emulsion; collagen was prepared into a 20 g / L collagen aqueous solution using deionized water.

[0064] (2) Take collagen aqueous solution, adjust the pH value to 5.5-6.0 with 1M hydrochloric acid solution, stir for 2 hours, continue stirring at 4-6℃ for 4 hours, and use a cell disruptor with 500W ultrasonic power to sonicate for 2 minutes to obtain collagen nano liquid.

[0065] (3) Take 50 mL of lecithin emulsion, sonicate at 300 W for 10 min, and slowly add 50 mL of HPMC aqueous solution and 50 mL of collagen nano liquid while sonicating. After mixing, freeze dry to obtain phospholipid-collagen nanoparticles.

[0066] According to the formula, hyaluronic acid, poly-L-lactic acid and phospholipid-collagen nanoparticles are mixed to obtain cosmetic and plastic surgery materials.

[0067] According to the detection method of Example 2, the cosmetic surgery material prepared in Example 3 showed an expression level of 1.033 for type IV collagen in the 3D skin anti-aging model.

[0068] Comparative Example 1

[0069] A cosmetic surgery material, with the following formula: 30% hyaluronic acid, 30% poly-L-lactic acid, and 40% collagen nanoparticles;

[0070] The preparation method of collagen nanoparticles is as follows:

[0071] (1) Prepare a collagen aqueous solution of 20 g / L using deionized water;

[0072] (2) Take collagen aqueous solution, adjust the pH value to 5.5-6.0 with 1M hydrochloric acid solution, stir for 2 hours, continue stirring at 4-6℃ for 4 hours, and use a cell disruptor with 500W ultrasonic power to sonicate for 2 minutes to obtain collagen nano liquid.

[0073] (3) The collagen nano liquid was freeze-dried to obtain collagen nanoparticles.

[0074] According to the formula, hyaluronic acid, poly-L-lactic acid and collagen nanoparticles are mixed to obtain cosmetic and plastic surgery materials.

[0075] According to the detection method of Example 2, the cosmetic surgery material prepared in Comparative Example 1 showed an expression level of 0.778 for type IV collagen in the 3D skin anti-aging model.

[0076] Example 4

[0077] Subject assessment:

[0078] Sample: Cosmetic and plastic surgery material prepared according to formula 2.

[0079] Subjects: 38 female subjects aged 30 to 45 years were selected. Volunteers who wished to achieve facial rejuvenation through injection of cosmetic materials and had not undergone cosmetic surgery were included. Those who were allergic to drugs or experimental equipment, had liver or kidney dysfunction or blood disorders, had facial deformities or incomplete development of facial features, or whose doctors determined that the injection method was not suitable for treatment were excluded.

[0080] Test environment: Temperature 20.0℃~22.0℃, relative humidity 40%~60%.

[0081] Treatment Method: After the subject undergoes makeup removal and cleansing, anesthesia is administered to the injection sites. The treating physician assesses the subject's facial condition and skin condition, marks the treatment areas, and performs bone and skin contouring separately. Before injection, disinfection is performed, and needles are inserted into the predetermined injection sites. Small amounts of cosmetic material are slowly injected into the nasal base, periorbital area, and lips, while multiple injection points are used for correction to achieve the final treatment effect. After injection, the needles are withdrawn, gentle pressure is applied for shaping, and ice is applied for 15 minutes.

[0082] Subjects were followed up and evaluated at 1, 2, and 6 months post-surgery.

[0083] Rating criteria: The maximum score is 7 points, with 1 point indicating strong disagreement; 2 points indicating disagreement; 3 points indicating some disagreement; 4 points indicating neutral or average; 5 points indicating some agreement; 6 points indicating agreement; and 7 points indicating strong agreement.

[0084] Table 3 Subject assessment results

[0085]

[0086] Table 3 shows that 30 days post-surgery, the subjects scored 5.55 for improved skin elasticity, 5.48 for improved skin wrinkles, 5.77 for improved skin hydration, 1.74 for facial redness, 1.06 for skin tingling, and 1.13 for skin itching or other discomfort. A few subjects experienced facial flushing and mild itching 1-3 days post-surgery. At 60 and 180 days post-surgery, the subjects' scores for improved skin elasticity, skin wrinkles, and skin hydration all showed varying degrees of improvement. Some subjects reported improvements in facial depressions and sagging, improved facial contours, and firmer skin, with noticeable and lasting effects.

[0087] None of the subjects experienced significant discomfort during the treatment process, and no other adverse reactions occurred after the procedure.

[0088] Typical Case

[0089] Ms. Zhou, 34 years old, requested: to appear youthful and natural, and to reduce fatigue.

[0090] 1. Preoperative facial baseline analysis of the patient was performed as follows:

[0091] advantage:

[0092] ① Harmonious proportions of the three courts

[0093] ② Delicate features

[0094] ③ A narrow face shape makes the face appear smaller.

[0095] shortcoming:

[0096] ① Sunken temples on both sides

[0097] ② Periorbital depression, obvious dark circles and tear troughs

[0098] ③ Prominent nasolabial folds, indicating fatigue

[0099] ④ The lines of the chin and jawline are not smoothly connected.

[0100] 2. The treatment plan is as follows:

[0101] (1) Bone structure shaping

[0102] ① Nasal base depression (injection dosage):

[0103] • 0.25 mL / side of the nasal triangle

[0104] • 0.2 mL / side at the base of the nasal ala

[0105] ·0.1 mL at the base of the nostril

[0106] ·0.1 mL at the base of the nasal columella

[0107] • Nasolabial folds 0.3mL

[0108] Raise the nasal base, reduce nasolabial folds, and smooth the mid-face.

[0109] ② Periorbital depression:

[0110] • Lateral orbital support fixation 0.2 mL

[0111] 0.2 mL of inferior orbital bone

[0112] · 0.3mL of brow bone

[0113] It improves periorbital three-dimensionality, enhances T-zone three-dimensionality, reduces periorbital fatigue, and results in good facial contour parameters for the patient.

[0114] (2) Skin preparation (injection dosage)

[0115] Under-eye bags: 0.02mL for the first segment, 0.05mL for the middle segment, 0.02mL for the last segment, and 0.01mL for the extended last segment (smooths under-eye area / reduces tear troughs / shortens the midface / graceful look);

[0116] Lips: 0.05mL / side for cupid's bow + 0.1mL for corner lifting + 1.4mL for deep lower lip support + 0.2mL for lower lip shaping (fuller lips, shorter philtrum, and more youthful appearance).

[0117] Follow-up results: The patient recovered well after the surgery. Wrinkles and folds on the mid and lower face were significantly reduced, the three-dimensionality of the periorbital and T-zone was improved, the nasolabial folds and tear troughs became shallower, the jawline curve was clearly visible, and the facial skin was full and shiny. No other serious complications occurred, and the patient was satisfied with the surgical lifting effect.

[0118] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A cosmetic surgery material, characterized in that, By weight percentage, it comprises the following components: 20%–40% hyaluronic acid, 20%–40% poly-L-lactic acid, and 30%–50% phospholipid-collagen nanoparticles; The preparation method of the phospholipid-collagen nanoparticles includes adding collagen nano liquid and hydroxypropyl methylcellulose aqueous solution to lecithin emulsion under ultrasonic action, and drying to obtain the product; The collagen nanofluid is obtained by stirring an aqueous collagen solution under acidic conditions and then breaking it down.

2. The cosmetic surgery material according to claim 1, characterized in that, By weight percentage, it comprises the following components: 25%–40% hyaluronic acid, 25%–40% poly-L-lactic acid, and 30%–45% phospholipid-collagen nanoparticles.

3. The cosmetic surgery material according to claim 2, characterized in that, By weight percentage, it comprises the following components: 25%–35% hyaluronic acid, 30%–40% poly-L-lactic acid, and 30%–40% phospholipid-collagen nanoparticles.

4. The cosmetic surgery material according to claim 3, characterized in that, The cosmetic surgery material comprises, by weight percentage: 30% hyaluronic acid, 30% poly-L-lactic acid, and 40% phospholipid-collagen nanoparticles; Alternatively, by weight percentage, 30% hyaluronic acid, 35% poly-L-lactic acid, and 35% phospholipid-collagen nanoparticles; Alternatively, by weight percentage, it contains 30% hyaluronic acid, 40% poly-L-lactic acid, and 30% phospholipid-collagen nanoparticles.

5. The cosmetic surgery material according to claim 1, characterized in that, The preparation method of the collagen nanofluid includes: adjusting the pH of the collagen aqueous solution to 5.5-6.0 with hydrochloric acid, stirring for the first time, placing it at 4-6℃ for a second stirring, and ultrasonically breaking it down to obtain the collagen nanofluid.

6. The cosmetic surgery material according to claim 5, characterized in that, The first stirring time is 2-3 hours, and the second stirring time is 4-5 hours; The ultrasonic fragmentation process takes 2–4 minutes and the ultrasonic power is 400–600 W. The mass concentration of the collagen aqueous solution is 18–22 g / L.

7. The cosmetic surgery material according to claim 1, characterized in that, The preparation method of the phospholipid-collagen nanoparticles includes: sonicating a lecithin emulsion, adding collagen nanoparticles and an aqueous solution of hydroxypropyl methylcellulose while sonicating, mixing, and then freeze-drying to obtain phospholipid-collagen nanoparticles; The volume ratio of the collagen nanofluid, the hydroxypropyl methylcellulose aqueous solution, and the lecithin emulsion is 9–11:9–11:4–6. The ultrasound duration is 10–20 minutes, and the ultrasound power is 300–400 W. The lecithin emulsion has a mass concentration of 8–12 g / L, and the hydroxypropyl methylcellulose aqueous solution has a mass concentration of 18–22 g / L.

8. A method for preparing a cosmetic surgery material according to any one of claims 1 to 7, characterized in that, According to the formula, hyaluronic acid, poly-L-lactic acid and phospholipid-collagen nanoparticles are mixed to obtain cosmetic and plastic surgery materials.

9. The cosmetic material according to any one of claims 1 to 7 includes its use in anti-aging, wrinkle removal, and skin tightening.

10. The application according to claim 9, characterized in that, The cosmetic materials include applications in enhancing type IV collagen expression.

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