Fusion protein and application thereof in preparation of phosphatidic acid biosensor

By designing fusion proteins to prepare phosphatidic acid biosensors, intracellular phosphatidic acid can be monitored in real time using changes in fluorescence signals. This solves the problem of real-time detection in existing technologies and achieves high sensitivity and low background detection.

CN120842429APending Publication Date: 2025-10-28SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Patent Information

Application Number
CN202510782652.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-12
Publication Date
2025-10-28

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Abstract

The invention relates to the technical field of biological detection, and particularly discloses a fusion protein and application thereof in preparation of a phosphatidic acid biosensor. The fusion protein comprises circulating arrangement fluorescent protein, Nir1-N protein located at the N end of the circulating arrangement fluorescent protein and Nir1-C protein located at the C end of the circulating arrangement fluorescent protein. The amino acid sequence of the Nir1-N protein is as shown in SEQ ID NO. 1, and the amino acid sequence of the Nir1-C protein is as shown in SEQ ID NO. 2; the cyclically arranged fluorescent protein is selected from cyclically arranged green fluorescent protein, cyclically arranged yellow fluorescent protein, cyclically arranged Venus fluorescent protein and cyclically arranged blue fluorescent protein. The Nir1-N and the Nir1-C in the fusion protein can be combined with phosphatidic acid, and when the phosphatidic acid is combined with the Nir1-N and the Nir1-C, the fluorescent protein is circularly arranged to generate conformational change, so that a fluorescence signal is changed, and detection is realized.
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Citation Information

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