Composite adsorption material for uremia and preparation method thereof

Abstract

The invention belongs to the technical field of adsorbents, and provides a composite adsorption material for uremia and a preparation method of the composite adsorption material for uremia. The preparation method comprises the following steps: mixing N, N '-methylene bisacrylamide, glycerol and deionized water, stirring, adding sodium alginate, stirring for dissolving, sequentially adding ammonium persulfate, calcium sulfate and tetramethylethylenediamine, stirring, performing ultrasonic treatment, and heating for reaction to obtain a sodium alginate derivative; s2, mixing a sodium alginate derivative and deionized water, adding modified high-density polyethylene, stirring, and soaking in a CaCl2 solution for crosslinking to obtain a sodium alginate composite material; s3, mixing the CeO2 / MoS2 functional material, sodium alginate and deionized water, adding the sodium alginate composite material, stirring, forming, soaking in a CaCl2 solution for crosslinking, washing and drying to obtain the composite adsorption material. The composite adsorption material disclosed by the invention is high in adsorption rate and high in adsorption capacity on uremia toxins, and has relatively good biocompatibility and blood compatibility.

Description

Technical Field

[0001] This invention belongs to the field of adsorbent technology, specifically relating to a composite adsorbent material for uremia and its preparation method. Background Technology

[0002] Uremia, the end-stage manifestation of kidney failure, has become a major disease threatening human health in recent years. Currently, common treatments for uremia include kidney transplantation, hemodialysis, hemoperfusion, and peritoneal dialysis. With the continuous development of dialysis technology, wearable artificial kidneys have brought new hope to uremia patients. Wearable artificial kidneys (WAKs) significantly reduce dialysate volume (<500 mL) through a closed-loop "dialysis-adsorption" cycle. Their core bottleneck lies in the lack of a "high-capacity, high-selectivity, periodically replaceable" adsorbent to achieve multiple regenerations of dialysate, thereby improving the convenience and cost-effectiveness of treatment. However, traditional adsorbent materials such as activated carbon, zirconium phosphate, and zirconium oxide have poor adsorption selectivity for small-molecule toxins like urea and creatinine, which are produced in large quantities through metabolism, making it difficult to meet clinical needs.

[0003] Molybdenum disulfide (MoS2) is a nanomaterial with a unique layered structure, exhibiting broad application potential in various fields due to its distinctive physicochemical properties. In the field of artificial kidney technology, the adsorption performance of molybdenum disulfide has attracted particular attention, mainly due to its large specific surface area and abundant active sites at its edges, theoretically enabling efficient adsorption of uremic toxins. However, the direct application of molybdenum disulfide in artificial kidney technology still faces many challenges, such as insufficient biostability, poor biocompatibility, and selective adsorption bottlenecks.

[0004] Chinese patent CN 118751214 A discloses a method for preparing an aminated bacterial cellulose / molybdenum disulfide composite adsorbent, the adsorbent itself, and its application. The method includes the following steps: bacterial cellulose, sodium molybdate, and thiourea are placed in an aqueous solution and subjected to a hydrothermal reaction; the mixture is then washed and dried to obtain a bacterial cellulose / molybdenum disulfide nanocomposite material; PEI is added to the bacterial cellulose / molybdenum disulfide dispersion aqueous solution and stirred until homogeneous; the mixture is then washed and dried to obtain the composite adsorbent. This composite adsorbent exhibits excellent heavy metal ion adsorption performance, low preparation cost, and good stability. However, the adsorption effect of this composite adsorbent on uremic toxins is unknown. Heavy metal ions are soft acids or interface acids, which can form stable metal-sulfur coordination bonds with exposed sulfur vacancies or edge sulfur atoms on the surface of MoS2. Uremic toxins (urea, creatinine, etc.) are mostly neutral or weakly polar molecules, and cannot achieve efficient adsorption through similar coordination interactions. The selective adsorption performance and applicability still need further investigation.

[0005] Therefore, there is an urgent need to develop a composite adsorbent material for uremia that has excellent adsorption performance and good adsorption stability for uremic toxins. Summary of the Invention

[0006] To address the existing technical problems, the present invention aims to provide a composite adsorbent material for uremia and its preparation method. The composite adsorbent material of the present invention exhibits excellent mechanical stability, can withstand long-term hydraulic shocks within the dialysis column, and demonstrates a rapid adsorption rate and high adsorption capacity for uremic toxins. It also possesses good biocompatibility and blood compatibility, and thus shows promising market prospects in the field of hemodialysis.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: This invention provides a method for preparing a composite adsorbent material for uremia, comprising the following steps: S1. Mix acrylamide, N,N'-methylenebisacrylamide, glycerol and deionized water, stir, add sodium alginate, stir to dissolve, then add ammonium persulfate, calcium sulfate and tetramethylethylenediamine in sequence, stir, sonicate, heat to 55-70℃ and react for 1-2 hours to obtain sodium alginate derivative. S2. Mix the sodium alginate derivative obtained in step S1 with deionized water, add modified high-density polyethylene, stir, and then soak it in a 2.5-4wt% CaCl2 solution for crosslinking for 1.5-2.5h to obtain sodium alginate composite material. S3. Mix CeO2 / MoS2 functional material, sodium alginate, and deionized water, add the sodium alginate composite material obtained in step S2 and stir. After forming, soak it in 2.5-4wt% CaCl2 solution for crosslinking for 1.5-2.5h, wash, freeze dry, and obtain composite adsorbent material.

[0008] The reaction mechanism and function of this invention are as follows: Sodium alginate is a biopolymer extracted from natural brown algae. It has abundant hydroxyl and carboxyl groups, which can provide active sites for the adsorption of pollutants. However, sodium alginate has problems such as high brittleness, poor fatigue resistance, and poor creep resistance when used as an adsorbent material.

[0009] On the one hand, the applicant introduces acrylamide to prepare sodium alginate derivatives. After polymerization, acrylamide forms a long polyacrylamide chain, whose amide group (-CONH2) interacts with the carboxyl group (-COO) of sodium alginate. - Through hydrogen bonding / physical entanglement, an interpenetrating network is formed, transforming linear sodium alginate into a covalently cross-linked elastic network (sodium alginate-g-polyacrylamide). N,N'-methylenebisacrylamide, acting as a cross-linking agent, introduces covalent cross-linking points between the polyacrylamide chains, significantly improving gel elasticity and solving the problem of pure sodium alginate in Ca... 2+The cross-linked material exhibits high brittleness and fragility. However, while polyacrylamide can adsorb small molecule toxins such as urea and creatinine through hydrogen bonding, and its hydrophilic segments can absorb water, the covalent cross-linked network limits its unlimited expansion. This allows the material to adsorb toxins without clogging dialysis tubing in uremia blood purification. More importantly, the composite adsorbent material has negatively charged groups on its surface, reducing the adsorption of plasma proteins and platelets, thus improving blood compatibility.

[0010] On the other hand, the applicant introduced high-density polyethylene to improve the mechanical stability of sodium alginate. However, directly adding high-density polyethylene to water easily leads to agglomeration. Therefore, furanuronic acid-γ-lactone was used to modify it, making it uniformly dispersed. The modified high-density polyethylene provides a micron-scale rigid skeleton, which can effectively disperse stress, reduce crack propagation, and is not prone to creep deformation under long-term stress, thus improving the fatigue resistance and creep resistance of the sodium alginate composite material and extending the life of the composite adsorbent material.

[0011] In addition, the applicant combined molybdenum disulfide nanoparticles and cerium nitrate hexahydrate to form CeO2 / MoS2 nanomaterials using ultrasound. Subsequently, N-aminoethyl-3-aminopropyltriethoxysilane was modified on the active sites to facilitate functionalization. This not only increased the total specific surface area and adsorption sites of molybdenum disulfide, improving the adsorption rate and capacity of the adsorbent material for uremic toxins, but also reduced the cytotoxicity of molybdenum disulfide and improved biocompatibility.

[0012] In some embodiments, the mass ratio of acrylamide to sodium alginate in step S1 is (5-8):1.

[0013] In some embodiments, the mass ratio of sodium alginate derivative and modified high-density polyethylene in step S2 is 1:(0.5-1.3).

[0014] In some embodiments, the method for preparing modified high-density polyethylene in step S2 includes the following steps: Q1. Mix furanuronic acid-γ-lactone and deionized water, heat to 85-95℃, and stir for 1.5-3 hours to obtain the reaction solution; Q2. Add high-density polyethylene and a 3-7% potassium permanganate aqueous solution to a sealed high-pressure reactor, stir, heat to 65-80℃, react for 5-10 minutes, filter, wash, add the reaction solution obtained in step Q1 and p-toluenesulfonic acid, stir, heat to 140-150℃, react for 1-2 hours, filter, wash, and dry to obtain modified high-density polyethylene.

[0015] In some embodiments, the mass ratio of the high-density polyethylene to the potassium permanganate aqueous solution is 1:(0.7-1.4).

[0016] In some embodiments, the mass ratio of furanuronic acid-γ-lactone to high-density polyethylene is (0.35-0.6):1.

[0017] In some embodiments, the high-density polyethylene has a particle size of 60-200 mesh.

[0018] In some embodiments, the mass ratio of the CeO2 / MoS2 functional material, sodium alginate, and sodium alginate composite material in step S3 is (0.1-0.2):1:(1-2.5).

[0019] In some embodiments, the preparation method of the CeO2 / MoS2 functional material in step S3 includes the following steps: R1. Nano-molybdenum disulfide was prepared by hydrothermal reaction of ammonium molybdate tetrahydrate and thiourea. R2. Mix the nano-molybdenum disulfide, cerium nitrate hexahydrate, and deionized water obtained in step R1, disperse them by ultrasonication, add hydrochloric acid, heat to 90-95℃, stir the reaction for 10-25 min, cool, wash, and dry to obtain CeO2 / MoS2 nanomaterials. R3. Mix the CeO2 / MoS2 nanomaterials obtained in step R2, N-aminoethyl-3-aminopropyltriethoxysilane, anhydrous ethanol, and nitric acid, heat and sonicate, wash, and dry to obtain CeO2 / MoS2 functional materials.

[0020] In some implementations, step R1 specifically involves the following steps: Ammonium molybdate tetrahydrate, thiourea, and deionized water were added to a sealed reaction vessel, sonicated at 100-200W for 20-45 minutes, heated to 180-210℃, and reacted for 16-24 hours. After cooling, the mixture was centrifuged once, and the precipitate was washed 3-4 times with deionized water, then dispersed with deionized water, centrifuged a second time, and the supernatant was collected and dried to obtain nano-molybdenum disulfide.

[0021] In some embodiments, the ratio of CeO2 / MoS2 nanomaterial and N-aminoethyl-3-aminopropyltriethoxysilane in step R3 is 1 g: (6-10) mL.

[0022] In another aspect, the present invention provides a composite adsorbent material obtained by the above preparation method.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The composite adsorbent material of the present invention has good mechanical stability, can withstand long-term hydraulic shock in the dialysis column, and has a fast adsorption rate and high adsorption capacity for uremic toxins. It also has good biocompatibility and blood compatibility, and has good market prospects in the field of hemodialysis.

[0024] 2. This invention solves the problems of brittleness, poor fatigue resistance and creep resistance of sodium alginate by introducing acrylamide and modified high-density polyethylene, thereby improving mechanical stability. At the same time, the functional modification of CeO2 / MoS2 nanomaterials improves adsorption performance and biocompatibility. Detailed Implementation

[0025] The present invention will be described below with reference to specific embodiments. It should be noted that the following embodiments are examples of the present invention and are used only to illustrate the invention, not to limit it. Other combinations and various modifications within the scope of the present invention can be made without departing from its spirit or scope.

[0026] Each composite adsorbent was prepared according to the proportions and preparation methods of the raw materials specified in the following examples and comparative examples.

[0027] To facilitate implementation of this invention by those skilled in the art, the manufacturers of some raw materials for the embodiments and comparative examples are described below: Sodium alginate: purchased from Shaanxi Panlong Yihai Pharmaceutical Co., Ltd.; High-density polyethylene: purchased from Dongguan Nabaichuan Plastics Co., Ltd., particle size 100 mesh; Unless otherwise specified, all other raw materials can be purchased from the market.

[0028] Preparation Example 1 The preparation method of modified high-density polyethylene A includes the following steps: Q1. Mix 4.8g of furanuronic acid-γ-lactone with 200mL of deionized water, heat to 90℃, and stir for 2.5h to obtain the reaction solution; Q2. Add 10g of high-density polyethylene and 10.5g of 5% potassium permanganate aqueous solution to a sealed high-pressure reactor, stir evenly, heat to 70℃, react for 8min, filter, wash 3 times with deionized water, add the reaction solution obtained in step Q1 and 0.08g of p-toluenesulfonic acid, stir evenly, heat to 145℃ and react for 1.5h, filter, wash 3 times with deionized water first, then wash 3 times with ethanol, and vacuum dry at 90℃ and -0.05MPa for 10min to obtain modified high-density polyethylene A.

[0029] Preparation Example 2 The preparation method of modified high-density polyethylene B is the same as that in Preparation Example 1, except that the amount of 5% potassium permanganate aqueous solution added is 6.5g.

[0030] Preparation Example 3 The preparation method of modified high-density polyethylene C is the same as that in Preparation Example 1, except that the amount of furanuronic acid-γ-lactone added is 3.2g.

[0031] Preparation Example 4 The preparation method of CeO2 / MoS2 functional material A includes the following steps: R1. Add 1.5g ammonium molybdate tetrahydrate, 3.15g thiourea, and 50mL deionized water to a sealed reaction vessel, sonicate at 150W for 35min, heat to 200℃, react for 20h, cool to room temperature, centrifuge at 12000rpm for 30min, take the precipitate, wash it 4 times with deionized water, disperse it with deionized water, centrifuge at 4500rpm for 15min, take the supernatant, dry at 60℃ for 48h to obtain nano-molybdenum disulfide; R2. Mix 1g of nano-molybdenum disulfide, 0.37g of cerium nitrate hexahydrate, and 200ml of deionized water obtained in step R1, sonicate for 8min, add 5mL of 0.12mol / L hydrochloric acid, heat to 95℃, stir for 15min, cool to room temperature, wash 3 times with anhydrous ethanol, then wash 3 times with deionized water, and dry at 60℃ for 16h to obtain CeO2 / MoS2 nanomaterials. R3. Mix 1g of CeO2 / MoS2 nanomaterial obtained in step R2, 8mL of N-aminoethyl-3-aminopropyltriethoxysilane, 60mL of anhydrous ethanol, and 0.5mL of 30wt% nitric acid solution, heat to 60℃, sonicate at 150W for 20min, wash twice with deionized water, then wash twice with ethanol, and dry at 75℃ for 8h to obtain CeO2 / MoS2 functional material A.

[0032] Preparation Example 5 The preparation method of CeO2 / MoS2 functional material B includes the following steps: R1. Add 1.5g ammonium molybdate tetrahydrate, 3.15g thiourea, and 50mL deionized water to a sealed reaction vessel, sonicate at 150W for 35min, heat to 200℃, react for 20h, cool to room temperature, centrifuge at 12000rpm for 30min, take the precipitate, wash it 4 times with deionized water, disperse it with deionized water, centrifuge at 4500rpm for 15min, take the supernatant, dry at 60℃ for 48h to obtain nano-molybdenum disulfide; R2. Mix 1g of nano-molybdenum disulfide, 0.37g of cerium nitrate hexahydrate, and 200ml of deionized water obtained in step R1, sonicate for 8min, add 5mL of 0.12mol / L hydrochloric acid, heat to 95℃, stir for 15min, cool to room temperature, wash 3 times with anhydrous ethanol, then wash 3 times with deionized water, and dry at 60℃ for 16h to obtain CeO2 / MoS2 functional material B.

[0033] Preparation Example 6 The preparation method of CeO2 / MoS2 functional material C is the same as that in preparation example 4, except that the amount of N-aminoethyl-3-aminopropyltriethoxysilane added is 5.5 mL.

[0034] Example 1 A method for preparing a composite adsorbent material for uremia includes the following steps: S1. Mix 32.5g acrylamide, 0.15g N,N'-methylenebisacrylamide, 10mL glycerol and 275mL deionized water, stir well, add 5g sodium alginate, stir to dissolve, then add 0.1g ammonium persulfate, 1g calcium sulfate and 0.05mL tetramethylethylenediamine in sequence, stir well and sonicate for 5min, heat to 65℃ and react for 1.5h to obtain sodium alginate derivative; S2. Mix 3g of sodium alginate derivative obtained in step S1 with 30mL of deionized water, add 2.7g of modified high-density polyethylene A, stir evenly, and then soak it in 3wt% CaCl2 solution for crosslinking for 2h to obtain sodium alginate composite material. S3. Mix 0.45g CeO2 / MoS2 functional material A, 3g sodium alginate, and 100mL deionized water. Add 5.25g sodium alginate composite material obtained in step S2 and stir for 10min. Shape the mixture using a hydrophobic PTFE microporous plate (pore size 0.8 cm) mold. Then, immerse the mixture in a 3wt% CaCl2 solution for crosslinking for 2h. Wash the mixture three times with deionized water and freeze-dry it at -20℃ for 18h to obtain the composite adsorbent material.

[0035] Example 2 A method for preparing a composite adsorbent material for uremia includes the following steps: S1. Mix 25g acrylamide, 0.15g N,N'-methylenebisacrylamide, 10mL glycerol and 220mL deionized water, stir well, add 5g sodium alginate, stir to dissolve, then add 0.1g ammonium persulfate, 1g calcium sulfate and 0.05mL tetramethylethylenediamine in sequence, stir well and sonicate for 5min, heat to 70℃ and react for 1h to obtain sodium alginate derivative; S2. Mix 3g of sodium alginate derivative obtained in step S1 with 30mL of deionized water, add 1.5g of modified high-density polyethylene A, stir evenly, and then soak it in 2.5wt% CaCl2 solution for crosslinking for 2.5h to obtain sodium alginate composite material. S3. Mix 0.3g CeO2 / MoS2 functional material A, 3g sodium alginate, and 100mL deionized water. Add the 3g sodium alginate composite material obtained in step S2 and stir for 10min. Shape the mixture using a hydrophobic PTFE microporous plate (pore size 0.8 cm) mold. Then, immerse the mixture in a 2.5wt% CaCl2 solution for crosslinking for 2.5h. Wash the mixture three times with deionized water and freeze-dry it at -20℃ for 18h to obtain the composite adsorbent material.

[0036] Example 3 A method for preparing a composite adsorbent material for uremia includes the following steps: S1. Mix 40g acrylamide, 0.15g N,N'-methylenebisacrylamide, 10mL glycerol and 330mL deionized water, stir well, add 5g sodium alginate, stir to dissolve, then add 0.1g ammonium persulfate, 1g calcium sulfate and 0.05mL tetramethylethylenediamine in sequence, stir well and sonicate for 5min, heat to 55℃ and react for 2h to obtain sodium alginate derivative; S2. Mix 3g of sodium alginate derivative obtained in step S1 with 30mL of deionized water, add 3.9g of modified high-density polyethylene A, stir evenly, and then soak it in 4wt% CaCl2 solution for crosslinking for 1.5h to obtain sodium alginate composite material. S3. Mix 0.6g CeO2 / MoS2 functional material A, 3g sodium alginate, and 100mL deionized water. Add 7.5g sodium alginate composite material obtained in step S2 and stir for 10min. Shape the mixture using a hydrophobic PTFE microporous plate (pore size 0.8 cm) mold. Then, immerse the mixture in a 4wt% CaCl2 solution for crosslinking for 1.5h. Wash the mixture three times with deionized water and freeze-dry it at -20℃ for 18h to obtain the composite adsorbent material.

[0037] Example 4 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that an equal amount of modified high-density polyethylene B is used instead of modified high-density polyethylene A.

[0038] Example 5 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that an equal amount of modified high-density polyethylene C is used instead of modified high-density polyethylene A.

[0039] Example 6 The preparation method of a composite adsorbent for uremia is the same as in Example 1, except that an equal amount of CeO2 / MoS2 functional material B is used to replace CeO2 / MoS2 functional material A.

[0040] Example 7 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that an equal amount of CeO2 / MoS2 functional material C is used to replace CeO2 / MoS2 functional material A.

[0041] Example 8 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that the amount of acrylamide added is 22.5g.

[0042] Example 9 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that the amount of modified high-density polyethylene A added is 1.2g.

[0043] Example 10 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that the amount of CeO2 / MoS2 functional material A added is 0.15g.

[0044] Comparative Example 1 The preparation method of a composite adsorbent material for uremia is the same as in Example 1, except that an equal amount of commercially available high-density polyethylene is used instead of modified high-density polyethylene A.

[0045] Effect evaluation: The composite adsorbent materials prepared in Examples 1-10 and Comparative Example 1 were tested and analyzed. The specific results are shown in Tables 1-2.

[0046] Performance testing: I. Adsorption Performance Take 10 ml of plasma solution containing urea and creatinine respectively, add 5 g of the composite adsorbent material prepared in Examples 1-10 and Comparative Example 1, seal, and shake in a shaker at 37℃ and 150 rpm for 2 h and 24 h respectively. After the adsorption is completed, measure the changes of each toxin. Calculate the adsorption rate and mechanical stability of the adsorbent for each toxin based on the concentration difference before and after adsorption. Repeat three times and take the average value.

[0047] Table 1

[0048] As shown in Table 1, the composite adsorbent materials prepared in Examples 1-3 exhibit excellent adsorption performance for urea and creatinine, as well as good mechanical stability.

[0049] Compared to Example 1, Example 4 changed the mass ratio of high-density polyethylene and potassium permanganate aqueous solution when preparing modified high-density polyethylene, resulting in insufficient oxidation sites and the potential for leaving unoxidized crystalline regions, which is not conducive to the subsequent modification of furanuronic acid-γ-lactone. Example 5 changed the mass ratio of furanuronic acid-γ-lactone and high-density polyethylene when preparing modified high-density polyethylene, resulting in insufficient grafting on the surface of high-density polyethylene. Comparative Example 1 used an equal amount of commercially available high-density polyethylene to replace modified high-density polyethylene A. As a result, Examples 4-5 and Comparative Example 1 all resulted in low degree of modification of high-density polyethylene and poor dispersibility, ultimately leading to poor mechanical stability of the composite material. Long-term dialysis may cause cracks, which in turn affects the adsorption rate of the composite adsorbent material during long-term dialysis.

[0050] Compared to Example 1, Example 6 did not graft N-aminoethyl-3-aminopropyltriethoxysilane when preparing CeO2 / MoS2 functional materials. Example 7 changed the ratio of CeO2 / MoS2 composite nanomaterials and N-aminoethyl-3-aminopropyltriethoxysilane when preparing CeO2 / MoS2 functional materials. Both reduced the amino active groups, which not only reduced the adsorption of uremic toxins, but also decreased biocompatibility, thus affecting the stability during long-term dialysis.

[0051] Compared to Example 1, Example 8 changed the mass ratio of acrylamide and sodium alginate, resulting in insufficient effective sites, reduced adsorption rate, and decreased gel elasticity, making it more brittle and affecting the adsorption rate of the composite adsorbent material during long-term dialysis.

[0052] Example 9 altered the mass ratio of sodium alginate derivative and modified high-density polyethylene compared to Example 1, resulting in a sparse skeleton of the composite adsorbent material that is prone to creep and breakage, thus reducing the urea / creatinine clearance rate during long-term dialysis.

[0053] Compared to Example 1, Example 10 changed the mass ratio of CeO2 / MoS2 functional material, sodium alginate, and sodium alginate composite material, resulting in a significant decrease in adsorption capacity and scavenging rate.

[0054] II. Blood compatibility The composite adsorbents prepared in Examples 1-3 and 6-8 were soaked in physiological saline for 30 minutes, and the surface moisture was removed by filtration using a sand core funnel. 1 g of adsorbent was weighed and added to 5 ml of freshly provided human blood. The mixture was placed in a 37°C water bath for constant temperature. After 2 hours, the blood cells were removed and the changes in blood cells were measured using a blood cell analyzer. The process was repeated three times and the average value was taken.

[0055] Table 2

[0056] As shown in Table 2, the composite adsorbent materials prepared in Examples 1-3 have good blood compatibility.

[0057] Compared to Example 1, Example 6 did not graft N-aminoethyl-3-aminopropyltriethoxysilane when preparing CeO2 / MoS2 functional materials. Example 7 changed the ratio of CeO2 / MoS2 composite nanomaterials and N-aminoethyl-3-aminopropyltriethoxysilane when preparing CeO2 / MoS2 functional materials. Both reduced the amino active groups, reduced the negative charge shielding effect, and decreased blood compatibility.

[0058] Compared to Example 1, Example 8 changed the mass ratio of acrylamide and sodium alginate, resulting in poorer gel elasticity. The particles were more prone to breakage under blood flow or extracorporeal circulation shear, producing micron-sized debris, which in turn led to decreased blood compatibility.

[0059] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present application in any way. Although the present application discloses the preferred embodiment as described above, it is not intended to limit the present application. Any changes or modifications made by those skilled in the art without departing from the scope of the technical solution of the present application using the disclosed technical content are equivalent to equivalent implementation cases. Any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the scope of the technical solution of the present invention are still within the scope of the technical solution.

Claims

1. A method for preparing a composite adsorbent material for uremia, characterized in that, It includes the following steps: S1. Mix acrylamide, N,N'-methylenebisacrylamide, glycerol and deionized water, stir, add sodium alginate, stir to dissolve, then add ammonium persulfate, calcium sulfate and tetramethylethylenediamine in sequence, stir, sonicate, heat to 55-70℃ and react for 1-2 hours to obtain sodium alginate derivative. S2. Mix the sodium alginate derivative obtained in step S1 with deionized water, add modified high-density polyethylene, stir, and then soak it in a 2.5-4wt% CaCl2 solution for crosslinking for 1.5-2.5h to obtain sodium alginate composite material. S3. Mix CeO2 / MoS2 functional material, sodium alginate, and deionized water, add the sodium alginate composite material obtained in step S2 and stir. After forming, soak it in 2.5-4wt% CaCl2 solution for crosslinking for 1.5-2.5h, wash, freeze dry, and obtain composite adsorbent material.

2. The method for preparing a composite adsorbent material for uremia according to claim 1, characterized in that, The mass ratio of acrylamide to sodium alginate in step S1 is (5-8):

1.

3. The method for preparing a composite adsorbent material for uremia according to claim 1, characterized in that, The mass ratio of sodium alginate derivative and modified high-density polyethylene in step S2 is 1:(0.5-1.3).

4. The method for preparing a composite adsorbent material for uremia according to claim 1, characterized in that, The method for preparing modified high-density polyethylene in step S2 includes the following steps: Q1. Mix furanuronic acid-γ-lactone and deionized water, heat to 85-95℃, and stir for 1.5-3 hours to obtain the reaction solution; Q2. Add high-density polyethylene and a 3-7% potassium permanganate aqueous solution to a sealed high-pressure reactor, stir, heat to 65-80℃, react for 5-10 minutes, filter, wash, add the reaction solution obtained in step Q1 and p-toluenesulfonic acid, stir, heat to 140-150℃, react for 1-2 hours, filter, wash, and dry to obtain modified high-density polyethylene.

5. The method for preparing a composite adsorbent material for uremia according to claim 4, characterized in that, The mass ratio of the high-density polyethylene to the potassium permanganate aqueous solution is 1:(0.7-1.4).

6. The method for preparing a composite adsorbent material for uremia according to claim 4, characterized in that, The mass ratio of furanuronic acid-γ-lactone to high-density polyethylene is (0.35-0.6):

1.

7. The method for preparing a composite adsorbent material for uremia according to claim 1, characterized in that, The mass ratio of CeO2 / MoS2 functional material, sodium alginate and sodium alginate composite material in step S3 is (0.1-0.2):1:(1-2.5).

8. The method for preparing a composite adsorbent material for uremia according to claim 1, characterized in that, The preparation method of the CeO2 / MoS2 functional material in step S3 includes the following steps: R1. Nano-molybdenum disulfide was prepared by hydrothermal reaction of ammonium molybdate tetrahydrate and thiourea. R2. Mix the nano-molybdenum disulfide, cerium nitrate hexahydrate, and deionized water obtained in step R1, disperse them by ultrasonication, add hydrochloric acid, heat to 90-95℃, stir the reaction for 10-25 min, cool, wash, and dry to obtain CeO2 / MoS2 nanomaterials. R3. Mix the CeO2 / MoS2 nanomaterials obtained in step R2, N-aminoethyl-3-aminopropyltriethoxysilane, anhydrous ethanol, and nitric acid, heat and sonicate, wash, and dry to obtain CeO2 / MoS2 functional materials.

9. The method for preparing a composite adsorbent material for uremia according to claim 8, characterized in that, In step R3, the ratio of CeO2 / MoS2 nanomaterials to N-aminoethyl-3-aminopropyltriethoxysilane is 1 g: (6-10) mL.

10. A composite adsorbent material obtained by the preparation method according to any one of claims 1-9.

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