Application of saposhnikovia divaricata c2' h gene and method for increasing coumarin content in saposhnikovia divaricata

By cloning the SdC2'H gene of Saposhnikovia divaricata and overexpressing it in hairy roots, the regulatory problem of coumarin synthesis in Saposhnikovia divaricata was solved, and the coumarin content was significantly increased, laying the technical foundation for efficient production.

CN120866338BActive Publication Date: 2026-08-04JILIN AGRICULTURAL UNIV
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Patent Information

Application Number
CN202510776103.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-06-11
Publication Date
2026-08-04
Estimated Expiration
2045-06-11

AI Technical Summary

Technical Problem

Current technology has not yet successfully cloned the C2'H gene in Saposhnikovia divaricata, and the lack of gene function verification and coumarin synthesis regulation methods hinders the improvement of the material basis of Saposhnikovia divaricata's medicinal efficacy.

Method used

The SdC2'H gene of Saposhnikovia divaricata was successfully cloned, and its gene overexpression vector was constructed. Saposhnikovia divaricata cotyledons were transformed by Agrobacterium rhizogenes, which induced the formation of hairy roots overexpressing the SdC2'H gene and improved the catalytic efficiency of coumarin synthesis.

Benefits of technology

It significantly increased the coumarin content in hairy roots, confirming that the SdC2'H gene positively regulates coumarin biosynthesis, and providing a basis for the genetic engineering production of high-value coumarin.

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Abstract

The application discloses application of a SdC2'H gene of Saposhnikovia divaricata and a method for increasing coumarin content in Saposhnikovia divaricata, and belongs to the technical field of bio-agriculture. The SdC2'H gene of Saposhnikovia divaricata and the biological material containing the SdC2'H gene are applied to the regulation of coumarin synthesis in Saposhnikovia divaricata. The method for increasing coumarin content in Saposhnikovia divaricata comprises the steps of increasing the expression amount and / or activity of the SdC2'H gene in Saposhnikovia divaricata. The SdC2'H gene is successfully cloned from Saposhnikovia divaricata for the first time, and a gene overexpression vector is constructed. The SdC2'H gene is positively regulated to control the synthesis of coumarin for the first time, and overexpression of the SdC2'H gene can significantly increase the product content. This not only deepens the understanding of the molecular mechanism of related biosynthesis, but also lays an important foundation for efficient production of high-value coumarin by using genetic engineering technology.
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