Light source device and automatic switching method of microscope light source

Abstract

The embodiment of the invention provides a light source device and an automatic switching method of a microscope light source, and relates to the field of microscopes. The light source device comprises a base, a fluorescent light source module, a rotary switching assembly and a control system, the fluorescent light source module is arranged on the base, and the fluorescent light source module can generate fluorescent light with different wavelengths; the rotary switching assembly is arranged on the base, the rotary switching assembly is provided with a plurality of fluorescent channels, and the control system is in communication connection with the fluorescent light source module and the rotary switching assembly; the control system can be used for controlling the fluorescent light source module to generate fluorescent light, and the control system can be used for controlling the rotation switching assembly to rotate, so that the fluorescent light with different wavelengths generated by the fluorescent light source module can respectively correspond to different fluorescent channels, excitation observation is carried out on different fluorescent markers, and the problem of tedious manual switching operation is avoided. The embodiment of the invention also provides an automatic switching method of the microscope light source, which is realized through the light source device.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microscope, in particular to a light source device and an automatic switching method of microscope light source. BACKGROUND

[0002] Fluorescent labeling technology is widely used in biomedical research, molecular detection and imaging field. Different markers have significant differences in excitation light and emission light wavelength range due to the difference in chemical structure, for example, blue fluorescent protein needs near ultraviolet light excitation. Different fluorescent markers need to be excited by light of corresponding wavelength for observation. In a fluorescence microscope, different fluorescence channels are usually switched to observe different fluorescent markers.

[0003] Traditional light source switching mode usually relies on manual replacement of filters to complete, and manual operation has problems such as tediousness, slow switching speed, and low precision. SUMMARY

[0004] The present application provides a light source device and an automatic switching method of microscope light source, which can replace manual switching of light source, realize automatic switching, and improve switching speed and precision.

[0005] Embodiments of the present application can be implemented as follows: Embodiments of the present application provide a light source device, which comprises: a base; a fluorescent light source module, the fluorescent light source module being arranged on the base and capable of generating fluorescent light of different wavelengths; a rotary switching assembly, the rotary switching assembly being arranged on the base and having a plurality of fluorescent channels; a control system, the control system being arranged on the base, in communication with the fluorescent light source module, and capable of controlling the fluorescent light source module to generate fluorescent light; the control system is also in communication with the rotary switching assembly and capable of controlling the rotary switching assembly to rotate.

[0006] Optionally, the base comprises a receiving cavity, the rotary switching assembly is installed in the receiving cavity and can rotate along the Z-axis, and the fluorescent light source module is arranged outside the rotary switching assembly.

[0007] Optionally, the fluorescent light source module comprises a fluorescent lamp panel, a moving driving mechanism, and a side shell, the moving driving mechanism is connected to the side shell, the side shell is connected to the base, the fluorescent lamp panel is connected to the moving driving mechanism, and a plurality of lamp beads of different wavelengths are arranged on the fluorescent lamp panel; the moving driving mechanism can drive the fluorescent lamp panel to move, so that the lamp beads of different wavelengths correspond to different fluorescent channels.

[0008] Optionally, the moving driving mechanism comprises a stepping motor, a guide rail, a screw rod and a sliding block, the screw rod is connected with the stepping motor, the guide rail is arranged along the screw rod, the sliding block is sleeved on the guide rail in a slidable mode and is threadedly connected with the screw rod, and the fluorescent lamp plate is connected with the sliding block.

[0009] Optionally, the base is further provided with a collimating lens, one end of the collimating lens is aligned with the fluorescent lamp plate, and the other end of the collimating lens is aligned with the rotating switching assembly.

[0010] Optionally, the rotating switching assembly comprises a rotating driving member, a rotating disc, a rotating disc seat and a plurality of fluorescent excitation blocks, the plurality of fluorescent excitation blocks are distributed in a circumferential direction on the rotating disc seat, the rotating disc is connected with the rotating disc seat, the rotating disc is provided with a plurality of fluorescent channels, the plurality of fluorescent channels correspond to the plurality of fluorescent excitation blocks respectively, the rotating disc seat is connected with the rotating driving member, and the rotating driving member is connected in communication with the control system.

[0011] Optionally, the rotating disc seat is provided with a triggering member, the base is provided with a plurality of photoelectric switches, the plurality of photoelectric switches are distributed in a circumferential direction below the rotating disc seat, and the plurality of photoelectric switches are connected in communication with the control system.

[0012] Optionally, an outer peripheral wall of the rotating disc seat is provided with a limiting groove, and the base is provided with a sheet limiting assembly, the sheet limiting assembly can be clamped with the limiting groove to position the rotating disc seat.

[0013] Optionally, the base is further provided with a white light source module, and the white light source module is connected in communication with the control system.

[0014] The embodiment of the present application also provides an automatic switching method of a microscope light source, which is realized through the light source device and comprises the following steps. Placing a detection object with a fluorescent marker in a fluorescent channel observation position; According to a fluorescent species on the fluorescent marker, the control system controls the fluorescent light source module to generate fluorescent light of a corresponding wavelength; The control system controls the rotating switching assembly to rotate, so that the fluorescent light of the corresponding wavelength passes through the corresponding fluorescent channel and irradiates on the detection object with the fluorescent marker, for observation by a person.

[0015] The embodiment of the present application has the following beneficial effects: The light source device comprises a base, a fluorescent light source module, a rotary switching assembly and a control system. The fluorescent light source module is arranged on the base and can generate fluorescent light of different wavelengths. The rotary switching assembly is arranged on the base and has multiple fluorescent light channels. The control system is arranged on the base and is in communication connection with the fluorescent light source module and the rotary switching assembly. The control system can be used to control the circuit connection of the fluorescent light source module to generate fluorescent light. The control system can also be used to control the rotation of the rotary switching assembly, so that the different wavelengths of fluorescent light generated by the fluorescent light source module can correspond to different fluorescent light channels respectively, thereby exciting and observing different fluorescent markers, and avoiding the problem of complicated manual switching operation.

[0016] The automatic switching method of the microscope light source comprises the following steps: placing a detection object with a fluorescent marker in a fluorescent light channel observation position; according to the type of fluorescent light on the fluorescent marker, the control system controls the fluorescent light source module to generate fluorescent light of a corresponding wavelength; the control system controls the rotary switching assembly to rotate, so that the fluorescent light of the corresponding wavelength passes through the corresponding fluorescent light channel and irradiates on the detection object with the fluorescent marker for observation by a person. The switching method is realized according to the light source device described above, and has all the functions of the light source device. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0018] Figure 1 The overall structure schematic diagram of the light source device provided in the embodiments of the present application is shown in the figure. Figure 2 The arrangement schematic diagram of the rotary switching assembly and the base provided in the embodiments of the present application is shown in the figure. Figure 3 The exploded schematic diagram of the rotary switching assembly provided in the embodiments of the present application is shown in the figure. Figure 4 The structure schematic diagram of the spring sheet limiting assembly provided in the embodiments of the present application is shown in the figure. Figure 5 The arrangement schematic diagram of the fluorescent light source module and the base provided in the embodiments of the present application is shown in the figure. Figure 6 The structure schematic diagram of the fluorescent light source module provided in the embodiments of the present application is shown in the figure.

[0019] Icon: 1-base; 10-housing cavity; 2-fluorescent light source module; 20-fluorescent lamp plate; 21-moving drive mechanism; 211-step motor; 212-guide rail; 213-screw rod; 214-sliding block; 22-side shell; 221-first connecting plate; 222-second connecting plate; 23-radiation plate; 24-collimating lens; 25-light source module connecting plate; 26-photoelectric proximity switch; 3-rotary switching assembly; 30-rotating disc; 301-fluorescent channel; 302-lightless channel; 31-rotating disc seat; 311-trigger; 312-limiting groove; 32-fluorescent excitation block; 33-base plate; 331-rotating shaft; 34-rotating disc bearing; 35-positioning connecting piece; 4-photoelectric circuit board; 40-photoelectric switch; 5-elastic piece limiting assembly; 50-torsion strut; 501-roller; 51-connecting block; 52-elastic piece; 53-pivot; 54-stop block; 6-white light source module; 7-observation carrier plate; 70-observation hole. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions and advantages of the embodiments of the present application clearer, the following will be a clear and complete description of the technical solutions in the embodiments of the present application in conjunction with the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the accompanying drawings can be arranged and designed in various different configurations.

[0021] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative labor are within the scope of protection of the present application.

[0022] It should be noted that: similar reference numerals and letters represent similar items in the following drawings, thus, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0023] In the description of the present application, it should be noted that if the terms "upper", "lower", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship when the product of the present application is usually placed, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0024] In addition, if the terms "first", "second" and the like appear, they are only used for differentiation in description, and cannot be understood as indicating or implying relative importance.

[0025] The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can also include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises... a" does not, without more constraints, exclude the presence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0026] Unless otherwise defined, the terms "set", "connected", and "coupled" are to be construed in accordance with their plain meaning in the context of the application, for example, "connected" can be a fixed connection, or a detachable connection, or an integral connection; can be a mechanical connection, or an electrical connection; can be a direct connection, or an indirect connection through an intermediate medium; can be a communication between the internal of two elements. For those skilled in the art, the specific meaning of the above terms in the application can be understood according to the specific circumstances.

[0027] It should be noted that, for the foregoing various method embodiments, in order to simply describe, they are all expressed as a combination of a series of actions, but those skilled in the art should know that the application is not limited by the order of the described actions, because according to the application, some steps can be performed in other order or simultaneously. The steps in the method of the embodiments of the application can be adjusted in order, combined and deleted according to actual needs.

[0028] It should be noted that the features in the embodiments of the application can be combined with each other without conflict.

[0029] As described in the background, different fluorescent markers need to be observed at corresponding wavelengths of fluorescent excitation, and in a fluorescence microscope, different fluorescence channels usually need to be switched to observe different fluorescent markers. Traditional light source switching methods usually rely on manual replacement of filters to complete, and manual operation has problems such as tediousness, slow switching speed, and low precision. Moreover, when switching to bright field observation, the fluorescent light source usually needs to be manually turned off and the white light source needs to be turned on, increasing the complexity of operation.

[0030] In view of the above problems, the embodiments of the application provide a light source device and a method for automatically switching microscope light sources, which can solve the above problems, which will be described in detail as follows.

[0031] Reference should be made to Figures 1 to 3The light source device comprises a base 1, a fluorescent light source module 2, a rotating switching assembly 3 and a control system. The fluorescent light source module 2 is arranged on the base 1 and can generate fluorescent light of different wavelengths. The rotating switching assembly 3 is arranged on the base 1 and has a plurality of fluorescent light channels 301. The control system is arranged on the base 1 and is in communication connection with the fluorescent light source module 2 and the rotating switching assembly 3. The control system can be used to control the circuit of the fluorescent light source module 2 to generate fluorescent light. The control system can also be used to control the rotation of the rotating switching assembly 3, so that the different wavelengths of fluorescent light generated by the fluorescent light source module 2 can correspond to different fluorescent light channels 301, thereby exciting and observing different fluorescent markers. The switching of the different wavelengths of fluorescent light and the switching of the fluorescent light channels 301 are all controlled by the control system, which is automatic and avoids the problems of complicated manual switching operation, slow switching speed and low switching precision.

[0032] In this embodiment, the base 1 is an integrated shell having a receiving cavity 10. The rotating switching assembly 3 is installed in the receiving cavity 10 and can rotate along the Z-axis. The fluorescent light source module 2 is arranged outside the rotating switching assembly 3, and the fluorescent light generated by the fluorescent light source module 2 can irradiate into the fluorescent light channels 301 of the rotating switching assembly 3.

[0033] Specifically, the rotating switching assembly 3 is arranged on a base plate 33, the base plate 33 is connected with the base 1, and the base plate 33 is provided with a rotating shaft 331 along the Z-axis direction. A cavity is left between the base plate 33 and the base 1, and a rotating drive member can be arranged in the cavity. The rotating drive member can be a driving motor. The output shaft of the driving motor is connected with the rotating shaft 331 on the base plate 33 through a shaft connector. When the driving motor works, it drives the rotating shaft 331 to rotate. Of course, the driving motor is in communication connection with the control system and receives the working instructions of the control system. The rotating switching assembly 3 is arranged above the base plate 33 and is connected with the rotating shaft 331. When the rotating shaft 331 rotates, the rotating switching assembly 3 also rotates.

[0034] Reference Figure 3 The rotating switching assembly 3 comprises a rotating drive member (not shown in the figure), a rotating disc 30, a rotating disc seat 31 and a plurality of fluorescent excitation blocks 32. The rotating disc 30 and the rotating disc seat 31 are connected together through positioning connectors 35. The positioning connectors 35 are rectangular blocks, and a plurality of screw holes are arranged at the upper and lower ends of the rectangular blocks. A plurality of screw holes are also arranged on the rotating disc 30 and the rotating disc seat 31. The rotating disc 30 and the rectangular blocks, and the rotating disc seat 31 and the rectangular blocks can be connected together by using screws / bolts. The rotating disc 30 and the rotating disc seat 31 are concentrically arranged, and the plurality of positioning connectors 35 are arranged at equal intervals on the rotating disc seat 31.

[0035] The rotating disc 30 is provided with a plurality of through holes which are spaced and uniformly distributed on the rotating disc 30, part of the plurality of through holes serve as fluorescent channels 301, and the remaining through holes serve as light-free channels 302, which means that no fluorescent light enters the channel in the bright field environment of the white light source. The plurality of fluorescent channels 301 correspond to a plurality of fluorescent excitation blocks 32 respectively, and each fluorescent channel 301 is arranged below one fluorescent excitation block 32, and the plurality of fluorescent excitation blocks 32 are distributed circumferentially on the rotating disc seat 31; it is worth mentioning that the fluorescent excitation block 32 is also called a fluorescent filter group, which is used to screen excitation light of a specific wavelength. For example, each fluorescent excitation block 32 can screen different wavelengths of fluorescent light, the A excitation block can screen a excitation light, and the B excitation block can screen b excitation light, when the a excitation light enters the A excitation block, it can be screened through, and when the b excitation light enters the A excitation block, it is filtered out. Therefore, the plurality of fluorescent excitation blocks 32 in the embodiment can screen different excitation light, and different excitation light can excite different fluorescent markers.

[0036] The rotating disc seat 31 is provided below with a rotating disc bearing 34 which is sleeved outside the rotating shaft 331, the upper side of the rotating disc bearing 34 is fixedly connected with the rotating disc seat 31, and the lower half of the rotating disc bearing 34 is fixed on the base plate 33, the rotating shaft 331 is fixedly connected with the rotating disc seat 31, and when the rotating drive works, it can drive the rotating shaft 331 to rotate, the rotating shaft 331 drives the rotating disc seat 31 and the plurality of fluorescent excitation blocks 32 arranged on the rotating disc seat 31 and the rotating disc 30 connected with the rotating disc seat 31 to rotate together, and since the rotating disc bearing 34 is arranged, the rotating disc seat 31 and the base plate 33 can rotate relative to each other. Of course, the rotating drive is in communication connection with the control system, and the start and stop of the rotating drive can be controlled through the control system.

[0037] The rotating disc seat 31 is provided with a trigger 311, the base 1 is provided with a photoelectric circuit board 4, a plurality of photoelectric switches 40 are arranged on the photoelectric circuit board 4 in a spaced manner, and the plurality of photoelectric switches 40 are circumferentially distributed below the rotating disc seat 31, when the trigger 311 triggers the target photoelectric switch 40, the rotating drive stops running, at this time, the target fluorescent light emitted by the fluorescent light source module 2 is aligned with the matching fluorescent excitation block 32 for use. The plurality of photoelectric switches 40 are in communication connection with the control system, and the target photoelectric switch 40 can be selected through the control system. The photoelectric switch 40 is composed of a transmitter, a receiver and a detection circuit, the transmitter emits light beams continuously to the target, the front end of the receiver is provided with a lens and an aperture, the receiver is connected with the detection circuit, the light beams emitted by the transmitter are converted into electric signals after passing through the lens and the aperture, and the detection circuit can analyze and apply the electric signals or filter out effective signals.

[0038] In order to accurately brake to control the target fluorescence to align the matched fluorescence excitation block 32, the outer peripheral wall of the rotating disc base 31 is provided with a plurality of limiting grooves 312, and the plurality of limiting grooves 312 are arranged corresponding to the fluorescence excitation block 32. The base plate 33 is provided with a spring limiting assembly 5, which can be clamped with the limiting groove 312 to position the rotating disc base 31 when the rotating disc base 31 rotates to the target position, preventing the rotating disc base 31 from rotating during observation.

[0039] Reference Figure 4 The spring limiting assembly 5 includes a torsion strut 50, a connecting block 51, a spring 52, a pin shaft 53 and a stop block 54. The connecting block 51 is connected with the base plate 33, the torsion strut 50 is arranged on the upper part of the connecting block 51, and one end of the torsion strut 50 is connected with the connecting block 51 through the pin shaft 53, and the torsion strut 50 can rotate around the pin shaft 53. The stop block 54 is arranged on the side of the torsion strut 50 and is fixedly connected with the connecting block 51. The stop block 54 is connected with the side of the torsion strut 50 close to the pin shaft 53 and is arranged with the spring 52. The spring 52 can be selected as a spring, which is arranged between the stop block 54 and the torsion strut 50. When the torsion strut 50 rotates under external force driving, the spring will be compressed. When the torsion strut 50 loses external force driving, the compressed spring will push the torsion strut 50 back to the original position.

[0040] Specifically, the end of the torsion strut 50 away from the pin shaft 53 is provided with a roller 501, which is movably connected with the outer peripheral wall of the rotating disc base 31. When the rotating disc base 31 rotates, the roller 501 can roll along the outer peripheral wall of the rotating disc base 31. When the roller 501 rolls to the limiting groove 312 of the rotating disc base 31, the end of the torsion strut 50 can be clamped in the limiting groove 312. At this time, the rotating disc base 31 stops rotating, so as to accurately control the stop position of the rotating disc base 31, and accurately make the target fluorescence generated by the fluorescence light source module 2 align the matched fluorescence excitation block 32, so as to achieve the purpose of fast and accurate switching.

[0041] Reference Figure 5 With Figure 6 The fluorescence light source module 2 is arranged on the side of the base 1 and vertically arranged. The fluorescence light source module 2 includes a fluorescence lamp plate 20, a moving driving mechanism 21 and a side shell 22. The moving driving mechanism 21 is connected with the side shell 22, the side shell 22 is connected with the base 1, and the fluorescence lamp plate 20 is connected with the moving driving mechanism 21. A plurality of lamp beads with different wavelengths are arranged on the fluorescence lamp plate 20. The moving driving mechanism 21 can drive the fluorescence lamp plate 20 to move, so that the lamp beads with different wavelengths correspond to different fluorescence channels 301. The side shell 22 and the base 1 are further provided with a light source module connecting plate 25, and the fluorescence light source module 2 is positioned and connected through the light source module connecting plate 25.

[0042] Specifically, the moving driving mechanism 21 comprises a stepping motor 211, a guide rail 212, a screw rod 213 and a sliding block 214. The screw rod 213 is connected with the stepping motor 211. The guide rail 212 is arranged below the screw rod 213 and along the length direction of the screw rod 213. The sliding block 214 has a through threaded hole. The lower part of the sliding block 214 is sleeved on the guide rail 212. The through threaded hole of the sliding block 214 is for the screw rod 213 to pass through. The inner side of the side shell 22 is provided with a first connecting plate 221 and a second connecting plate 222. The first connecting plate 221 and the second connecting plate 222 are arranged in parallel and spaced apart. The stepping motor 211 is fixed on the first connecting plate 221. The two ends of the screw rod 213 are rotatably connected with the first connecting plate 221 and the second connecting plate 222 through bearings. When the stepping motor 211 works, the screw rod 213 is driven to rotate. The screw rod 213 drives the sliding block 214 to slide along the guide rail 212. Since the fluorescent lamp plate 20 is connected with the sliding block 214, the fluorescent lamp plate 20 also moves with the sliding block 214. The fluorescent lamp plate 20 is provided with a plurality of lamp beads of different wavelengths. The movement of the fluorescent lamp plate 20 can make the fluorescent light emitted by the lamp bead with the target wavelength irradiate on the matched fluorescent excitation block 32.

[0043] The lamp beads on the fluorescent lamp plate 20 will generate a large amount of heat in the process of emitting fluorescent light. In order to avoid the heat generated by the lamp beads from being transmitted to the stepping motor 211, the sliding block 214 is further provided with a heat dissipation plate 23. The fluorescent lamp plate 20 is installed on the heat dissipation plate 23. The heat dissipation plate 23 is in a U-shaped structure. The heat dissipation plate 23 and the sliding block 214 have a heat dissipation interval region. Of course, the heat dissipation plate 23 can also be made of a material with poor heat conductivity. In this way, the heat generated by the fluorescent lamp plate 20 cannot be transmitted to the stepping motor 211.

[0044] Optionally, the base 1 is further provided with a collimator lens 24. One end of the collimator lens 24 is aligned with the fluorescent lamp plate 20. The other end of the collimator lens 24 is aligned with the fluorescent excitation block 32 of the rotating switching assembly 3. When the target lamp bead on the fluorescent lamp plate 20 is aligned with the matched fluorescent excitation block 32, the fluorescent light generated by the lamp bead irradiates into the fluorescent excitation block 32 after passing through the collimator lens 24. The collimator lens 24 can parallelly converge the fluorescent light generated by the lamp bead. The irradiation direction of the fluorescent light entering the fluorescent channel 301 is the same.

[0045] In order to enable the fluorescent lamp plate 20 to automatically return to the initial position after the detection is completed, a photoelectric proximity switch 26 is further arranged on the side shell 22, the photoelectric proximity switch 26 is arranged on the moving path of the heat dissipation plate 23, and the photoelectric proximity switch 26 is further in communication connection with the control system. When the heat dissipation plate 23 blocks the photoelectric proximity switch 26 when moving, the photoelectric proximity switch 26 turns on the loop to transmit a signal to the control system, and the control system instructs the stepper motor 211 to reverse and drive the fluorescent lamp plate 20 to return to the initial position, so as to avoid the fluorescent lamp plate 20 from moving out of the illuminable range. Specifically, the side of the heat dissipation plate 23 is provided with a lug, and the lug can be used to block the photoelectric proximity switch 26.

[0046] Referring again to Figure 1 , the base 1 is further provided with a white light source module 6, the white light source module 6 can generate white light, and is used to provide a bright field observation environment. The white light source module 6 is in communication connection with the control system; the white light source module 6 includes a white light lamp, and a remote control switch is arranged on a line electrically connected with the white light lamp, the remote control switch is in communication connection with the control system, and the start and stop of the white light lamp is controlled.

[0047] It is worth mentioning that an observation carrier plate 7 is arranged above the rotating disc 30, the observation carrier plate 7 is fixedly connected with the base 1, an observation hole 70 is formed in the observation carrier plate 7, the observation hole 70 is located above the fluorescent channel 301 or the light-free channel 302, and the observation carrier plate 7 is arranged in a spaced mode with the rotating disc 30, so that different fluorescent channels 301 or light-free channels 302 can be aligned with the observation hole 70 when the rotating disc 30 rotates. In actual use, a detection object with a fluorescent marker can be placed at the observation hole 70, and the fluorescent light emitted through the fluorescent channel 301 can irradiate the detection object, so that a person can observe. The observation hole 70 herein does not refer to a through hole, but a hole cavity with a transparent partition layer.

[0048] In the embodiment, the control system is an operating system which can accept external input instructions and issue execution commands. An operator inputs an instruction, and then the control system analyzes the input instruction and issues an execution command to an execution component. For example, the control system includes a microprocessor, the microprocessor is electrically connected with the photoelectric circuit board 4, so that the signal of the photoelectric switch 40 can be accepted; the microprocessor is in communication connection with the driving motor and the stepper motor 211, and is used to control the start and stop of the driving motor and the stepper motor 211; the microprocessor is in communication connection with the on-off switch on the electric connection line bundle of the fluorescent lamp plate 20, and is used to control the light emission of a certain lamp bead on the fluorescent lamp plate 20; the microprocessor is in communication connection with the remote control switch on the line of the white light source module 6, and is used to control the light emission of the white light lamp. The microprocessor can be programmed by PLC to execute the input instruction and make a correct execution command. The microprocessor is further connected with an operation panel, and an operator can input an instruction according to the operation panel.

[0049] The working principle of the light source device of the embodiment of the present application is as follows: The user places the detection object with fluorescent markers on the observation hole 70 of the observation board 7, and then selects the fluorescence (the wavelength range of the fluorescence can excite the fluorescent markers) matched with the fluorescent markers through the operation panel; after the control system receives the instruction, the corresponding photoelectric switch 40 on the photoelectric circuit board 4 is started, and then the driving motor in the rotary switching component 3 is controlled to rotate, driving the rotary switching component 3 to rotate; when the trigger 311 triggers the started photoelectric switch 40, the driving motor is stopped urgently, and the limiting groove 312 of the rotary disc seat 31 is clamped by the torsion support rod 50; at this time, the fluorescence excitation block 32 that meets the target wavelength fluorescence is just aligned with the collimator lens 24; the control system controls the stepping motor 211 to rotate, driving the fluorescent lamp plate 20 to move, so that the fluorescent lamp beads on the fluorescent lamp plate 20 that can emit the fluorescence matched with the fluorescent markers are aligned with the collimator lens 24; at the same time, the on-off switch on the fluorescent lamp bead electric connection wire harness is turned on, the fluorescent lamp beads emit the target fluorescence, the target fluorescence is adjusted in angle by the collimator lens 24 and converges, and then enters the corresponding fluorescence channel 301 after being turned by the fluorescence excitation block 32, and the target fluorescence irradiates on the detection object with fluorescent markers.

[0050] When the fluorescence is not needed, the control system controls the corresponding photoelectric switch 40 on the photoelectric circuit board 4 to start, and then controls the driving motor in the rotary switching component 3 to rotate, driving the rotary switching component 3 to rotate; when the trigger 311 triggers the started photoelectric switch 40, the driving motor is stopped urgently, and the limiting groove 312 of the rotary disc seat 31 is clamped by the torsion support rod 50; at this time, the non-light channel 302 on the rotary disc 30 is just aligned with the observation hole 70; the control system controls the remote control switch of the white light source module 6, so that the white light lamp emits white light, thereby providing a bright field observation environment.

[0051] The light source device of the embodiment of the present application only needs to input the required wavelength range of the fluorescence or white light on the operation panel when in use, and the control system can automatically switch and complete some execution commands after receiving the instruction, so that the automation degree is high, and the problems of complicated operation, slow speed and poor precision in the past manual switching are solved.

[0052] The embodiment of the present application also provides an automatic switching method of a microscope light source, which is realized through the above light source device and includes the following steps: S1: placing the detection object with fluorescent markers on the observation position of the fluorescence channel 301; that is, placing the detection object with fluorescent markers on the observation hole 70, and inputting the required wavelength of the fluorescence or white light through the operation panel.

[0053] S2: According to the fluorescence species on the fluorescent marker, the control system controls the fluorescent light source module 2 to generate fluorescent light of the corresponding wavelength; the control system controls the fluorescent lamp beads capable of generating fluorescent light of the required wavelength to emit light, and moves the fluorescent lamp plate 20 to the appropriate position, so that the light-emitting fluorescent lamp beads are aligned with the collimator lens 24.

[0054] S3: The control system controls the rotation of the rotating switching component 3, so that the fluorescent light of the required wavelength enters the fluorescent channel 301 after passing through the matched fluorescent excitation block 32, and then irradiates the detection object with the fluorescent marker for observation by personnel. Of course, if it is required to select a bright field white light environment for observation, the control system controls the fluorescent lamp plate 20 to be closed and the white light of the white light source module 6 to be turned on; the control system controls the rotation of the rotating switching component 3, so that the light-free channel 302 is aligned with the observation hole 70, and the white light generated by the white light lamp can enter the light-free channel 302 through diffuse reflection, thereby improving the observation effect.

[0055] Among them, the control system controls the fluorescent light source module 2 / white light source module 6 to be performed alternatively, while the instructions of the fluorescent light source module 2 / white light source module 6 and the rotating switching component 3 can be performed simultaneously or in sequence (the rotating switching component 3 can be executed first, and then the fluorescent light source module 2 / white light source module 6 emits light; or the fluorescent light source module 2 / white light source module 6 can emit light first, and then the rotating switching component 3 rotates).

[0056] The automatic switching method of the microscope light source of the embodiment of the present application does not need manual focusing, solves the precision problem of the previous manual focusing, and improves the stability and accuracy of the work of the fluorescent microscope.

[0057] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A light source device, characterized in that, include: Base (1); A fluorescent light source module (2) is disposed on the base (1), and the fluorescent light source module (2) is capable of generating fluorescence of different wavelengths; A rotating switching assembly (3) is disposed on the base (1) and has multiple fluorescence channels (301). The control system is located on the base (1) and is communicatively connected to the fluorescent light source module (2). The control system can be used to control the fluorescent light source module (2) to generate fluorescence. The control system is also communicatively connected to the rotation switching component (3). The control system can be used to control the rotation switching component (3) to rotate.

2. The light source device according to claim 1, characterized in that, The base (1) includes a receiving cavity (10), the rotary switching component (3) is installed in the receiving cavity (10), and the rotary switching component (3) can rotate along the Z-axis. The fluorescent light source module (2) is disposed on the outside of the rotary switching component (3).

3. The light source device according to claim 1, characterized in that, The fluorescent light source module (2) includes a fluorescent lamp plate (20), a moving drive mechanism (21), and a side shell (22). The moving drive mechanism (21) is connected to the side shell (22), and the side shell (22) is connected to the base (1). The fluorescent lamp plate (20) is connected to the moving drive mechanism (21). Multiple LED beads of different wavelengths are arranged on the fluorescent lamp plate (20). The moving drive mechanism (21) can drive the fluorescent lamp plate (20) to move, so that the LED beads of different wavelengths correspond to different fluorescent channels (301).

4. The light source device according to claim 3, characterized in that, The moving drive mechanism (21) includes a stepper motor (211), a guide rail (212), a lead screw (213), and a slider (214). The lead screw (213) is connected to the stepper motor (211). The guide rail (212) is arranged along the lead screw (213). The slider (214) is slidably sleeved on the guide rail (212) and threadedly connected to the lead screw (213). The fluorescent lamp board (20) is connected to the slider (214).

5. The light source device according to claim 3, characterized in that, The base (1) is also provided with a collimating lens (24), one end of which is aligned with the fluorescent lamp panel (20), and the other end of which is aligned with the rotating switching assembly (3).

6. The light source device according to claim 1, characterized in that, The rotary switching component (3) includes a rotary drive, a turntable (30), a turntable base (31), and multiple fluorescent excitation blocks (32). The multiple fluorescent excitation blocks (32) are circumferentially distributed on the turntable base (31). The turntable (30) is connected to the turntable base (31). The turntable (30) is provided with multiple fluorescent channels (301), and the multiple fluorescent channels (301) correspond to the multiple fluorescent excitation blocks (32). The turntable base (31) is connected to the rotary drive, and the rotary drive is communicatively connected to the control system.

7. The light source device according to claim 6, characterized in that, A trigger (311) is provided on the turntable base (31), and a plurality of photoelectric switches (40) are provided on the base (1). The plurality of photoelectric switches (40) are circumferentially distributed below the turntable base (31), and the plurality of photoelectric switches (40) are communicatively connected to the control system.

8. The light source device according to claim 6, characterized in that, The outer peripheral wall of the turntable seat (31) is provided with a limiting groove (312), and the base (1) is provided with a spring limiting component (5). The spring limiting component (5) can engage with the limiting groove (312) to position the turntable seat (31).

9. The light source device according to any one of claims 1-8, characterized in that, The base (1) is also provided with a white light source module (6), which is communicatively connected to the control system.

10. An automatic switching method for a microscope light source, implemented using the light source device according to any one of claims 1-9, characterized in that, Includes the following steps: Place the analyte with the fluorescent marker at the observation position in the fluorescence channel (301); According to the type of fluorescence on the fluorescent marker, the control system controls the fluorescent light source module (2) to generate fluorescence of the corresponding wavelength; The control system controls the rotation switching component (3) to rotate, so that the fluorescence of the corresponding wavelength is irradiated onto the test object with fluorescent marker through the corresponding fluorescence channel (301) for observation by personnel.

Images