Traditional Chinese medicine composition with anti-aging and life-prolonging effects as well as preparation method, product and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition with anti-aging and life-prolonging effects as well as a preparation method, a product and application thereof, and relates to the technical field of traditional Chinese medicines. The preparation method of the traditional Chinese medicine composition comprises the following steps: mixing the lucid ganoderma, the poria cocos, the prepared rehmannia root, the liquorice root, the Chinese angelica and the Chinese magnoliavine fruit, performing pretreatment, adjusting the pH value to 5.0-6.5, adding a compound enzyme 1, and performing mixing and inactivation at 30-50 DEG C to obtain a mixture 1; adjusting the pH value to 4.0-6.0, adding a compound enzyme 2, carrying out enzymolysis at 50-60 DEG C, and concentrating the enzymatic hydrolysate to obtain a component 1; mixing cortex cinnamomi and pericarpium citri reticulatae, performing steam distillation extraction, and concentrating an extracting solution to obtain a component 2; mixing the dregs with ginseng, rhizoma atractylodis macrocephalae, radix paeoniae alba, polygala tenuifolia and astragalus membranaceus, performing graded alcohol extraction, and concentrating an extracting solution to obtain a component 3; and mixing the component 1, the component 2 and the component 3, and drying to obtain the traditional Chinese medicine composition. The traditional Chinese medicine composition prepared by the invention has the effects of resisting aging, prolonging life and improving physical quality.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a traditional Chinese medicine composition with anti-aging and longevity-promoting effects, its preparation method, product, and application. Background Technology

[0002] Aging is a complex and gradual biological process, accompanied by impaired biological functions, a decline in the ability of cells or the body to respond to metabolic stress, and the development of age-related diseases such as diabetes, cancer, cardiovascular disease, neurodegenerative diseases, and increased risk of falls. With the development of modern society and economy, public awareness of health and wellness has been increasing. In the past few decades, delaying aging, improving health, and reducing the incidence of age-related diseases through anti-aging drugs have been the most effective interventions. Currently reported anti-aging molecules are mostly chemical drugs, but some of these drugs, while alleviating aging, can cause certain side effects. Therefore, finding safe and effective anti-aging and longevity-promoting compositions is a research hotspot in the field of traditional Chinese medicine.

[0003] Currently, there are reports of anti-aging and longevity-enhancing compositions. For example, Chinese patent application CN112021569A discloses an anti-aging, longevity-enhancing, and immunity-boosting composition with Astragalus membranaceus as the main ingredient. It is made from the following ingredients in parts by weight: 3-5 parts Astragalus membranaceus, 1-2 parts American ginseng, 2-3 parts Lycium barbarum, and 2-3 parts Polygonatum sibiricum. All the ingredients used in this anti-aging, longevity-enhancing, and immunity-enhancing composition are traditional Chinese medicinal herbs that are also used in food, making it a healthy, green, and safe anti-fatigue product. However, this invention does not present data on efficacy, and the strength of its anti-aging and longevity-enhancing effects is unknown.

[0004] Chinese patent application CN111254036A discloses a nourishing and kidney-tonifying wine and its preparation method, which consists of salvia miltiorrhiza, safflower, *Lycopodium clavatum*, *Taxillus chinensis*, *Rehmannia glutinosa*, *Ilex pubescens*, *Polygonum multiflorum*, *Epimedium brevicornu*, *Curcuma zedoaria*, *Sparganium stoloniferum*, mulberry twigs, dried tangerine peel, white hyacinth bean, leek seed, astragalus membranaceus, *Spatholobus suberectus*, malt, deer antler, *Spatholobus suberectus*, licorice root, fenugreek seed, *Poria cocos*, *Achyranthes bidentata*, yam, plantain seed, *Sinomenium acutum This health-preserving wine is made from at least one of the following ingredients: *Psoralea corylifolia*, *Psoralea corylifolia*, *Ligustrum lucidum*, deer heart blood powder, *Pteris vittata*, *Polygonum multiflorum*, deer tendon, white peony root, cassia seed, deer penis, *Cistanche deserticola*, *Alisma plantago-aquatica*, wolfberry, *Cynanchum paniculatum*, *Melia azedarach*, *Rosa laevigata*, *Ganoderma lucidum*, *Cyperus rotundus*, ginseng, gecko, seahorse, dog kidney, maca, *Cistanche deserticola*, tongkat ali, jujube, *Eucommia ulmoides*, *Aralia elata*, fennel, *Cornus officinalis*, *Dipsacus asper*, cinnamon, papaya, kudzu root, *Atractylodes macrocephala*, and mulberry. This health-preserving wine is said to invigorate qi and strengthen the spleen, clear the meridians, dispel wind and dampness, and alleviate lower back pain, benefit the liver and improve eyesight, and strengthen the body. However, on the one hand, the complex composition of this health-preserving wine may increase the metabolic burden on the liver and kidneys; on the other hand, this invention does not demonstrate any data on its anti-aging effects.

[0005] Zhibao Lingzhi wine is composed of Ganoderma lucidum, Panax ginseng, Atractylodes macrocephala, Poria cocos, Cinnamomum cassia, Citrus reticulata, Angelica sinensis (fried), Radix Paeoniae Alba, Radix Rehmanniae Recens, Astragalus membranaceus, Schisandra chinensis, Polygala, and Glycyrrhiza uralensis. It has the effects of tonifying qi and blood, soothing the nerves, and relieving asthma. It is used for treating deficiency of both qi and blood, pale or yellowish complexion, shortness of breath, cough with phlegm, fatigue of limbs, loss of appetite, palpitation, dizziness, and weakness after illness. In the prescription, Ganoderma lucidum tonifies qi and soothes the nerves, relieves cough and asthma; Panax ginseng greatly tonifies primordial qi, restores pulse and prevents collapse, tonifies the spleen and the lung, generates saliva and nourishes blood, and soothes the nerves and improves intelligence; Atractylodes macrocephala tonifies the spleen and benefits qi, dries dampness and promotes water; Poria cocos benefits the spleen, dries dampness, and calms the heart; Cinnamomum cassia tonifies fire and assists yang, leads fire back to the origin, dispels cold and relieves pain, and warms meridians; Citrus reticulata regulates qi and tonifies the spleen, dries dampness and reduces phlegm; Angelica sinensis tonifies blood, regulates menstruation, relieves pain, moistens the intestines and promotes defecation; Radix Paeoniae Alba tonifies blood, regulates menstruation, astringes yin, relieves sweating, softens the liver, relieves pain, and restrains liver yang; Radix Rehmanniae Recens tonifies blood and yin, and benefits essence and marrow; Astragalus membranaceus tonifies qi and raises yang, consolidates the exterior and stops sweating, promotes water and reduces swelling, generates saliva and nourishes blood, removes stagnation and relieves impediment, astringes and promotes pus discharge, and promotes tissue regeneration; Schisandra chinensis astringes and contracts, benefits qi and generates saliva, tonifies the kidney and calms the heart; Polygala soothes the nerves and improves intelligence, communicates between the heart and the kidney, relieves phlegm, and reduces swelling; and Glycyrrhiza uralensis tonifies the spleen and benefits qi, clears heat and removes toxicity, relieves phlegm and cough, relieves acute and chronic pain, and harmonizes various drugs. The preparation method is that the above thirteen ingredients are extracted with white wine under reflux, filtered, combined, and filtered, and the supernatant is absorbed, filtered, and sealed to obtain the product. However, the raw material ratio and the preparation method of the above preparation need to be further optimized.

[0006] Therefore, by optimizing the component ratio and the preparation method, a traditional Chinese medicine composition with the effects of resisting aging and prolonging life is developed, which is the research focus of researchers in the field. SUMMARY

[0007] The present application provides a traditional Chinese medicine composition with the effects of resisting aging and prolonging life and a preparation method thereof, which optimizes the raw material components, the component ratio, and the preparation method, and the prepared traditional Chinese medicine composition has the effects of resisting aging, prolonging life, and improving physical fitness.

[0008] To achieve the above-mentioned purposes, the technical solutions adopted by the present application are as follows: In a first aspect, the present application provides a preparation method of a traditional Chinese medicine composition with the effects of resisting aging and prolonging life, which comprises the following steps: S1: Ganoderma lucidum, Poria cocos, Radix Rehmanniae Recens, Glycyrrhiza uralensis, Angelica sinensis, and Schisandra chinensis are mixed for pretreatment, the pH is adjusted to 5.0-6.5, a composite enzyme 1 is added, and mixed at 30-50℃, and then inactivated to obtain a mixture 1; the composite enzyme 1 is selected from at least two of cellulase, pectin esterase, beta-1, 6-glucosidase, and chitinase; S2: the mixture 1 of step S1 is adjusted to a pH of 4.0-6.0, a composite enzyme 2 is added, and enzymolysis is carried out at 50-60℃, and then the enzymolysis liquid is concentrated to obtain component 1; the composite enzyme 2 is selected from at least two of cellulase, pectin esterase, beta-1, 6-glucosidase, and chitinase; S3: mixing cinnamon and dried tangerine peel, water vapor distillation extraction, and concentrating the extract to obtain component 2; S4: mixing the residue of step S3 with ginseng, atractylodes, white peony root, polygala and astragalus, fractionally extracting with alcohol, and concentrating the extract to obtain component 3; S5: mixing component 1, component 2 and component 3, and drying to obtain the traditional Chinese medicine composition.

[0009] Preferably, in step S1, the ganoderma lucidum, poria cocos, prepared rehmannia root, licorice, angelica and schisandra chinensis are first washed and crushed.

[0010] Preferably, in step S1, the specific operation of the pretreatment is adjusting the pH to 2.0-4.0, and stirring at 30-50℃ for 0.5-2h.

[0011] Further preferably, in step S1, the specific operation of the pretreatment is adjusting the pH to 2.0-3.0, and stirring at 30-40℃ for 1-2h.

[0012] Preferably, in step S1, the complex enzyme 1 is a mixture of cellulase and pectin esterase. Preferably, the mass ratio of the cellulase and the pectin esterase is 3-6:2-4. Further preferably, the mass ratio of the cellulase and the pectin esterase is 5:3.

[0013] The enzyme activity of the complex enzyme 1 is 10000-50000U / g; further preferably, the enzyme activity of the complex enzyme 1 is 35000U / g.

[0014] Preferably, in step S2, the complex enzyme 2 is a mixture of β-1,6-glucosidase and chitinase. Preferably, the mass ratio of the β-1,6-glucosidase and the chitinase is 0.8-1.2:0.8-1.2; further preferably, the mass ratio of the β-1,6-glucosidase and the chitinase is 1:0.9.

[0015] Preferably, the enzyme activity of the complex enzyme 2 is 60000-100000U / g; further preferably, the enzyme activity of the complex enzyme 2 is 100000U / g.

[0016] Preferably, in step S2, the enzymolysis time is 1-3h. Further preferably, in step S2, the enzymolysis time is 1.5h.

[0017] Preferably, in step S3, the particle size of the cinnamon and dried tangerine peel is 30-60μm. Preferably, in step S3, the water vapor distillation extraction is performed for 1-2 hours at a temperature of 130-170℃; further preferably, in step S3, the water vapor distillation extraction is performed for 2 hours at a temperature of 150℃.

[0018] Preferably, in step S4, the graded alcohol extraction uses ethanol solution.

[0019] Preferably, in step S4, the graded alcohol extraction is performed as follows: (1) the raw material is mixed with 30wt%-50wt% ethanol solution, and extraction is performed at 20-30℃ for 3-5 hours, and the extract is collected; (2) the concentration of the ethanol solution is increased to 60wt%-80wt%, and extraction is performed by heating to reflux for 1-2 hours, and the extract is collected; (3) the concentration of the ethanol solution is increased to 90wt%-95wt%, and extraction is performed at 50-60℃ for 1-2 hours, and the extract is collected; (4) the extracts of steps (1)-(3) are combined.

[0020] In a second aspect, the present application provides a traditional Chinese medicine composition prepared by the preparation method described above.

[0021] Preferably, the composition comprises the following components by mass fraction: Ganoderma lucidum 20-40 parts, ginseng 15-30 parts, white atractylodes 1-13 parts, Poria cocos 10-25 parts, cassia bark 10-20 parts, dried tangerine or orange peel 10-25 parts, Chinese angelica (fried) 15-28 parts, white peony root 1-13 parts, prepared rehmannia 5-18 parts, milk vetch 20-30 parts, Schisandra chinensis 10-20 parts, polygala 1-10 parts, and licorice 10-20 parts.

[0022] Preferably, the composition comprises the following components by mass fraction: Ganoderma lucidum 25-35 parts, ginseng 20-25 parts, white atractylodes 1-10 parts, Poria cocos 15-25 parts, cassia bark 10-18 parts, dried tangerine or orange peel 10-20 parts, Chinese angelica (fried) 20-28 parts, white peony root 5-13 parts, prepared rehmannia 5-15 parts, milk vetch 22-28 parts, Schisandra chinensis 10-18 parts, polygala 1-5 parts, and licorice 10-15 parts.

[0023] Further preferably, the composition comprises the following components by mass fraction: Ganoderma lucidum 30 parts, ginseng 22 parts, white atractylodes 6 parts, Poria cocos 18 parts, cassia bark 15 parts, dried tangerine or orange peel 18 parts, Chinese angelica (fried) 28 parts, white peony root 13 parts, prepared rehmannia 10 parts, milk vetch 25 parts, Schisandra chinensis 15 parts, polygala 3 parts, and licorice 10 parts.

[0024] In a third aspect, the present application provides a product comprising the traditional Chinese medicine composition prepared by the preparation method described above, and the product is a medicine or a health product.

[0025] Further preferably, the product is a medicine.

[0026] More preferably, the medicine is a medicinal liquor.

[0027] In a fourth aspect, the present application provides use of the traditional Chinese medicine composition prepared by the preparation method in the preparation of an anti-aging and life-prolonging product.

[0028] Compared with the prior art, the present application has the following beneficial effects: The present application optimizes the ratio of components, and through a specific preparation method: the ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica sinensis, and schisandra chinensis are pretreated and then enzymolyzed, and the ultrasonic is used for assistance; the cassia bark and dried tangerine or orange peel are extracted by water vapor distillation, and then the residue is subjected to fractional alcohol extraction together with panax ginseng, atractylodes macrocephala koidz, radix paeoniae alba, polygala, and astragalus membranaceus, so that the traditional Chinese medicine composition prepared finally has the effects of anti-aging, life-prolonging, improving immunity, and improving physical quality. DETAILED DESCRIPTION

[0029] In order to make the technical means, creative characteristics, and purposes and effects realized by the present application easy to understand, the present application is further illustrated below in combination with specific embodiments, but the following embodiments are only preferred embodiments of the present application, and are not all. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor all belong to the protection scope of the present application. It is worth noting that the raw materials used in the present application are all ordinary commercially available products, and their sources are not specifically limited. The technologies and scientific terms used in the embodiments have the meanings commonly understood by those skilled in the art to which the present application belongs.

[0030] Embodiment 1 A traditional Chinese medicine composition with anti-aging and life-prolonging effects: According to the mass fraction, the following components are contained: ganoderma lucidum 30 parts, panax ginseng 22 parts, atractylodes macrocephala koidz 6 parts, poria cocos 18 parts, cassia bark 15 parts, dried tangerine or orange peel 18 parts, angelica sinensis (fried) 28 parts, radix paeoniae alba 13 parts, radix rehmanniae preparatae 10 parts, astragalus membranaceus 25 parts, schisandra chinensis 15 parts, polygala 3 parts, and licorice 10 parts.

[0031] The preparation method comprises the following steps: S1: the raw materials of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica sinensis, and schisandra chinensis are mixed, washed, crushed, the pH is adjusted to 3.0, and pretreated at 40℃ for 1h under stirring; The pH is adjusted to 6.0, compound enzyme 1 is added, mixed at 45℃, inactivated, and mixture 1 is obtained; The compound enzyme 1 is a mixture of cellulase and pectin esterase (mass ratio 5:3); the addition amount of compound enzyme 1 is 0.2% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica sinensis, and schisandra chinensis; S2: the mixture 1 of step S1 is adjusted to pH 5.5, complex enzyme 2 is added, and enzymolysis is carried out at 55℃ for 1.5h, the enzymolysis solution is concentrated, and component 1 is obtained; the complex enzyme 2 is a mixture of β-1, 6-glucosidase and chitinase (mass ratio is 1:0.9); The adding amount of the complex enzyme 2 is 0.3% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis; S3: after the cassia and dried tangerine peel are mixed and crushed, the particle size is controlled to be 30-60μm, water vapor distillation extraction is carried out for 2h, the temperature is 150℃, and the extraction solution is concentrated to obtain component 2; S4: the residue of step S3 is mixed with ginseng, atractylodes, radix paeoniae alba, polygala and astragalus, fractional alcohol extraction is carried out, the extraction solution is concentrated, and component 3 is obtained; The specific operation of the fractional alcohol extraction is as follows: (1) the raw materials are mixed with 45wt% ethanol solution, extraction is carried out at 25℃ for 4h, and the extraction solution is collected; (2) the concentration of the ethanol solution is increased to 75wt%, heating to reflux extraction is carried out for 1h, and the extraction solution is collected; (3) the concentration of the ethanol solution is increased to 92wt%, extraction is carried out at 55℃ for 1.5h, and the extraction solution is collected; (4) the extraction solutions of steps (1)-(3) are combined.

[0032] S5: components 1, 2 and 3 are mixed and dried to obtain a traditional Chinese medicine composition.

[0033] Example 2 A traditional Chinese medicine composition with anti-aging and longevity effect: According to the mass fraction, the following components are contained: ganoderma lucidum 20 parts, ginseng 30 parts, atractylodes 1 part, poria cocos 25 parts, cassia 10 parts, dried tangerine peel 25 parts, angelica (fried) 15 parts, radix paeoniae alba 1 part, radix rehmanniae preparata 5 parts, astragalus 30 parts, schisandra chinensis 20 parts, polygala 1 part and licorice 20 parts.

[0034] The preparation method comprises the following steps: S1: the raw materials ganoderma lucidum, poria cocos, radix rehmanniae preparata, licorice, angelica and schisandra chinensis are mixed, washed, crushed, adjusted to pH 2.0, and pretreated by stirring at 50℃ for 0.5h; adjusted to pH 6.5, complex enzyme 1 is added, mixed at 50℃, and inactivated to obtain a mixture 1; The complex enzyme 1 is a mixture of cellulase and pectinesterase (mass ratio is 3:4); the adding amount of the complex enzyme 1 is 0.3% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparata, licorice, angelica and schisandra chinensis; S2: the mixture 1 of step S1 is adjusted to pH 4.0, complex enzyme 2 is added, and enzymolysis is carried out at 50℃ for 1.0h, the enzymolysis solution is concentrated, and component 1 is obtained; the complex enzyme 2 is a mixture of β-1, 6-glucosidase and chitinase (mass ratio is 0.8:1.2); The adding amount of the complex enzyme 2 is 0.1% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis; S3: after the mixture of cassia and dried tangerine or orange peel is crushed, the particle size is controlled to be 30-60μm, water vapor distillation extraction is carried out for 1.5h, the temperature is 130℃, and the extraction solution is concentrated to obtain component 2; S4: the residue of step S3 is mixed with panax ginseng, atractylodes, radix paeoniae alba, polygala and astragalus, fractional alcohol extraction is carried out, the extraction solution is concentrated, and component 3 is obtained; The specific operation of the fractional alcohol extraction is as follows: (1) the raw materials are mixed with 30wt% ethanol solution, extraction is carried out at 30℃ for 3h, and the extraction solution is collected; (2) the concentration of the ethanol solution is increased to 80wt%, heating to reflux extraction is carried out for 1.5h, and the extraction solution is collected; (3) the concentration of the ethanol solution is increased to 90wt%, extraction is carried out at 50℃ for 1.0h, and the extraction solution is collected; (4) the extraction solutions of steps (1)-(3) are combined.

[0035] S5: components 1, 2 and 3 are mixed and dried to obtain the traditional Chinese medicine composition.

[0036] Example 3 A traditional Chinese medicine composition with anti-aging and longevity effect: According to the mass fraction, the following components are contained: ganoderma lucidum 40 parts, panax ginseng 15 parts, atractylodes 13 parts, poria cocos 10 parts, cassia 20 parts, dried tangerine or orange peel 10 parts, angelica (fried) 23 parts, radix paeoniae alba 8 parts, radix rehmanniae preparatae 18 parts, astragalus 20 parts, schisandra chinensis 10 parts, polygala 10 parts and licorice 12 parts.

[0037] The preparation method comprises the following steps: S1: the raw materials ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis are mixed, washed, crushed, adjusted to pH 4.0, and pretreated by stirring at 30℃ for 2h; adjusted to pH 5.0, complex enzyme 1 is added, mixed at 30℃, and inactivated to obtain mixture 1; The complex enzyme 1 is a mixture of cellulase and pectinesterase (mass ratio is 6:2); the adding amount of the complex enzyme 1 is 0.1% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis; S2: the mixture 1 of step S1 is adjusted to pH 6.0, complex enzyme 2 is added, and enzymolysis is carried out at 60℃ for 3h, the enzymolysis solution is concentrated, and component 1 is obtained; the complex enzyme 2 is a mixture of β-1, 6-glucosidase and chitinase (mass ratio is 1.2:0.8); The adding amount of the complex enzyme 2 is 0.5% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis; S3: after the cassia and dried tangerine peel are mixed and crushed, the particle size is controlled to be 30-60μm, water vapor distillation extraction is carried out for 2h, the temperature is 170℃, and the extraction solution is concentrated to obtain component 2; S4: the residue of step S3 is mixed with panax ginseng, atractylodes, radix paeoniae alba, polygala and astragalus, fractional alcohol extraction is carried out, the extraction solution is concentrated, and component 3 is obtained; The specific operation of the fractional alcohol extraction is as follows: (1) the raw materials are mixed with 50wt% ethanol solution, extraction is carried out at 20℃ for 5h, and the extraction solution is collected; (2) the concentration of the ethanol solution is increased to 60wt%, heating to reflux extraction is carried out for 2h, and the extraction solution is collected; (3) the concentration of the ethanol solution is increased to 95wt%, extraction is carried out at 60℃ for 2h, and the extraction solution is collected; (4) the extraction solutions of steps (1)-(3) are combined.

[0038] S5: components 1, 2 and 3 are mixed and dried to obtain the traditional Chinese medicine composition.

[0039] Comparative example 1 A traditional Chinese medicine composition with anti-aging and life prolonging effects: Compared with example 1, the raw materials are the same, and only the preparation method is changed, which is as follows: All raw materials are refluxed with an appropriate amount of cane sugar with white wine, filtered, the filtrate is combined, and then placed, the supernatant is sucked, filtered, and then sealed, and the traditional Chinese medicine composition is obtained.

[0040] Comparative example 2 A traditional Chinese medicine composition with anti-aging and life prolonging effects: Compared with example 1, the raw materials are the same, and only the preparation method is changed, which is as follows: S1: the raw materials ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis are mixed, washed, crushed, adjusted to pH 3.0, and pretreated by stirring at 40℃ for 1h; adjusted to pH 6.0, complex enzyme 2 is added, mixed at 45℃, and inactivated to obtain mixture 1; The complex enzyme 2 is a mixture of β-1, 6-glucosidase and chitinase (mass ratio is 1:0.9); the adding amount of the complex enzyme 2 is 0.2% of the total mass of ganoderma lucidum, poria cocos, radix rehmanniae preparatae, licorice, angelica and schisandra chinensis; S2: Mixture 1 from step S1 is adjusted to pH 5.5, compound enzyme 1 is added, and enzymatic hydrolysis is carried out at 55℃ for 1.5h. The hydrolysate is concentrated to obtain component 1. Compound enzyme 1 is a mixture of cellulase and pectin esterase (mass ratio of 5:3). The amount of compound enzyme 2 added is 0.3% of the total mass of Ganoderma lucidum, Poria cocos, Rehmannia glutinosa, Glycyrrhiza uralensis, Angelica sinensis and Schisandra chinensis; S3: Cinnamon and dried tangerine peel are mixed and pulverized, with the particle size controlled at 30-60μm. They are then extracted by steam distillation for 2 hours at 150℃. The extract is then concentrated to obtain component 2. S4: The residue from step S3 is mixed with ginseng, atractylodes macrocephala, white peony root, polygala tenuifolia and astragalus membranaceus, and subjected to graded alcohol extraction. The extract is then concentrated to obtain component 3. The specific operation of the fractional alcohol extraction is as follows: (1) The raw material was mixed with 45wt% ethanol solution and extracted at 25℃ for 4h, and the extract was collected; (2) Increase the concentration of the ethanol solution to 75 wt%, heat to reflux for 1 h, and collect the extract; (3) Increase the concentration of the ethanol solution to 92 wt%, extract at 55 °C for 1.5 h, and collect the extract; (4) Combine the extracts from steps (1)-(3).

[0041] S5: Mix components 1, 2, and 3, and dry them to obtain a traditional Chinese medicine composition.

[0042] Comparative Example 3 A traditional Chinese medicine composition with anti-aging and longevity-promoting effects: Compared with Example 1, the raw materials are the same, only the preparation method S3 is changed, as follows: S3: Cinnamon and dried tangerine peel are mixed and pulverized, with the particle size controlled at 80μm. They are then extracted by steam distillation for 2 hours at 100℃. The extract is then concentrated to obtain component 2.

[0043] The rest is the same as in Example 1.

[0044] Comparative Example 4 A traditional Chinese medicine composition with anti-aging and longevity-promoting effects: Compared with Example 1, the raw materials are the same, only the preparation method S4 is changed, as follows: S4: The residue from step S3 is mixed with ginseng, atractylodes macrocephala, white peony root, polygala tenuifolia and astragalus membranaceus, and subjected to graded alcohol extraction. The extract is then concentrated to obtain component 3. The specific operation of the fractional alcohol extraction is as follows: (1) The raw material was mixed with 20wt% ethanol solution and extracted at 25℃ for 4h, and the extract was collected; (2) Increase the concentration of the ethanol solution to 55 wt%, heat to reflux for 1 h, and collect the extract; (3) The concentration of the ethanol solution is increased to 85wt%, and extraction is carried out at 55℃ for 1.5h, and the extract is collected; (4) The extracts of steps (1)-(3) are combined.

[0045] Comparative Example 5 A traditional Chinese medicine composition having an anti-aging and life-prolonging effect: Compared with Example 1, only the mass fraction of the raw materials is changed, and the details are as follows: By mass fraction, the following components are included: Ganoderma lucidum 18 parts, Panax ginseng 35 parts, Atractylodes alba 15 parts, Poria cocos 5 parts, Cinnamomum cassia 25 parts, Citrus reticulata 5 parts, Angelica sinensis (fried) 10 parts, Radix paeoniae alba 20 parts, Radix rehmanniae praeparata 20 parts, Astragalus membranaceus 15 parts, Schisandra chinensis 25 parts, Polygala tenuifolia 15 parts, and Glycyrrhiza uralensis 5 parts.

[0046] The preparation method is consistent with that of Example 1.

[0047] Test Example 1 The influence of the traditional Chinese medicine composition prepared in the application on oxidative stress effect is evaluated by using fruit fly experiment: black fruit flies are fed with fruit fly culture medium (HB8590); A control group is set, and the drug groups (Example 1-Example 3 and Comparative Example 1-Comparative Example 5): the traditional Chinese medicine composition with a concentration of 30 mg / L is added to the culture medium, and the blank group is added with the same amount of sterile water.

[0048] (1) Fruit fly life experiment: 48 h eclosion black fruit fly adults are collected, and randomly divided into groups, 200 fruit flies per group, 9 groups, half male and half female. Fresh culture medium is replaced every 96 h. After the start of the experiment, the death number of fruit flies is recorded every day, until all fruit flies in each group die. The half death time, maximum life span, average life span and average life prolonging rate of fruit flies are calculated.

[0049] Average life prolonging rate (%) = (average life span of the drug group-average life span of the control group) / average life span of the control group x 100%.

[0050] The experimental data are shown in Table 1.

[0051] Table 1. Influence of traditional Chinese medicine composition on fruit fly life

[0052] Note: ## p<0.01, compared with the control group, # p<0.05, compared with the control group.

[0053] (2) Determination of catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA): The 48 h eclosion Drosophila melanogaster adults were collected and randomly divided into groups. Each group of fruit flies was 200, and there were 9 groups, half male and half female. Fresh culture medium was replaced every 96 h. On the 30th day after administration, 8% tissue homogenate was prepared with normal saline; centrifugation was performed at 4000 rpm for 10 min, and the CAT, SOD and MDA levels of each group of supernatant were determined according to the CAT, SOD and MDA kit instructions, respectively. The experimental data are shown in Table 2.

[0054] Table 2. Effect of traditional Chinese medicine composition on CAT, SOD and MDA in fruit flies

[0055] Note: ## p < 0.01, compared with the control group, # p < 0.05, compared with the control group.

[0056] From the data in Tables 1-2, it can be seen that the traditional Chinese medicine composition prepared in the present application can prolong the lifespan of fruit flies, increase the levels of CAT and SOD in fruit flies, reduce the content of lipid peroxide degradation product MDA, has good ROS resistance, and has good application potential in anti-aging and prevention and treatment of Alzheimer's disease.

[0057] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A method for preparing a traditional Chinese medicine composition having an anti-aging effect, characterized in that, Comprising the following steps: S1: Ganoderma lucidum, Poria cocos, Radix Rehmanniae Preparata, Glycyrrhiza uralensis, Angelica sinensis and Schisandra chinensis are mixed for pretreatment, pH is adjusted to 5.0-6.5, a composite enzyme 1 is added, mixed at 30-50℃, inactivated, and a mixture 1 is obtained; the composite enzyme 1 is selected from at least two of cellulase, pectinesterase, β-1, 6-glucosidase and chitinase; S2: the mixture 1 of step S1, pH is adjusted to 4.0-6.0, a composite enzyme 2 is added, enzymolysis is carried out at 50-60℃, the enzymolysis liquid is concentrated, and a component 1 is obtained; the composite enzyme 2 is selected from at least two of cellulase, pectinesterase, β-1, 6-glucosidase and chitinase; S3: Cinnamomum cassia and Pericarpium Citri Reticulatae are mixed, water vapor distillation extraction is carried out, and the extract is concentrated to obtain a component 2; S4: the residue of step S3 is mixed with Panax ginseng, Atractylodes macrocephala, Radix Paeoniae Alba, Polygala and Astragalus membranaceus, fractional alcohol extraction is carried out, the extract is concentrated, and a component 3 is obtained; S5: the component 1, the component 2 and the component 3 are mixed, dried, and a traditional Chinese medicine composition is obtained.

2. The production method according to claim 1, characterized by, In step S1, the specific operation of the pretreatment is adjusting pH to 2.0-4.0, stirring at 30-50℃ for 0.5-2h.

3. The preparation method according to claim 2, characterized in that, In step S1, the specific operation of the pretreatment is adjusting pH to 2.0-3.0, stirring at 30-40℃ for 1-2h.

4. The method of claim 1, wherein, In step S1, the composite enzyme 1 is a mixture of cellulase and pectinesterase.

5. The production method according to claim 4, characterized by, The mass ratio of the cellulase and the pectinesterase is 3-6:2-4.

6. The method of claim 1, wherein, In step S2, the composite enzyme 2 is a mixture of β-1, 6-glucosidase and chitinase.

7. The production method according to claim 6, characterized by, The mass ratio of the β-1, 6-glucosidase and the chitinase is 0.8-1.2:0.8-1.

2.

8. The method of claim 1, wherein, In step S2, the time of the enzymolysis is 1-3h.

9. The method of claim 1, wherein, In step S3, the particle size of the Cinnamomum cassia and Pericarpium Citri Reticulatae is 30-60μm.

10. The method of claim 1, wherein, In step S3, the time of the water vapor distillation extraction is 1-2h, and the temperature is 130-170℃.

11. The method of claim 1, wherein, In step S4, the alcohol of the fractional alcohol extraction is an ethanol solution.

12. The method of claim 11, wherein, In step S4, the specific operation of the fractional alcohol extraction is: (1) mixing the raw materials with 30wt%-50wt% of the ethanol solution, extracting at 20-30℃ for 3-5h, and collecting the extract; (2) increasing the concentration of the ethanol solution to 60wt%-80wt%, heating to reflux extraction for 1-2h, and collecting the extract; (3) increasing the concentration of the ethanol solution to 90wt%-95wt%, extracting at 50-60℃ for 1-2h, and collecting the extract; (4) combining the extracts of steps (1)-(3).

13. The traditional Chinese medicine composition prepared by the preparation method of any one of claims 1-12.

14. The traditional Chinese medicine composition according to claim 13, characterized in that, According to the mass fraction, it comprises the following components: Ganoderma lucidum 20-40 parts, Panax ginseng 15-30 parts, Atractylodes macrocephala 1-13 parts, Poria cocos 10-25 parts, Cinnamomum cassia 10-20 parts, Pericarpium Citri Reticulatae 10-25 parts, Angelica sinensis 15-28 parts, Radix Paeoniae Alba 1-13 parts, Radix Rehmanniae Preparata 5-18 parts, Astragalus membranaceus 20-30 parts, Schisandra chinensis 10-20 parts, Polygala 1-10 parts and Glycyrrhiza uralensis 10-20 parts.

15. The traditional Chinese medicine composition according to claim 14, characterized in that, By mass parts, comprising the following components: Ganoderma 25-35 parts, ginseng 20-25 parts, white atractylodes 1-10 parts, Poria cocos 15-25 parts, cassia bark 10-18 parts, dried orange peel 10-20 parts, angelica 20-28 parts, white peony root 5-13 parts, prepared rehmannia 5-15 parts, milk vetch 22-28 parts, Schisandra 10-18 parts, polygala 1-5 parts and licorice 10-15 parts.

16. A product characterized by, The traditional Chinese medicine composition prepared by the preparation method of any one of claims 1-12, wherein the product is a medicine or a health product.

17. Use of the traditional Chinese medicine composition prepared by the preparation method of any one of claims 1-12 in the preparation of an anti-aging and longevity product.

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