Plasma matrix bone block preparation assembly, mold production method and plasma matrix bone block preparation method

Abstract

The invention discloses a plasma matrix bone block preparation assembly, a mold production method and a plasma matrix bone block preparation method, the plasma matrix bone block preparation assembly comprises a first mold, a first groove is formed in the first mold, the inner surface roughness Rsm of the first groove is larger than or equal to 0.1 mu m and smaller than or equal to 0.2 mu m, and the first mold is a titanium part. The plasma matrix bone block preparation assembly provided by the invention can promote the formation of a fibrous protein network, accelerate the gel formation speed of the plasma matrix, enhance the cross-linking tightness of the plasma matrix and a bone substitute material, shorten the preparation time of the plasma matrix bone block, and improve the preparation efficiency and mechanical strength of the plasma matrix bone block; the clinical application scene of the plasma matrix bone block is expanded.

Description

Technical Field

[0001] This invention relates to the field of plasma matrix bone block production technology, and in particular to a plasma matrix bone block preparation component, a mold production method, and a plasma matrix bone block preparation method. Background Technology

[0002] Plasma matrix, a concentrated extract derived from human autologous blood, has attracted considerable attention as an emerging bioregenerative material. It possesses excellent biocompatibility and biosafety, and does not induce immune rejection. The main component of plasma matrix is ​​platelets, which, upon successful activation, exert a powerful procoagulant effect. More importantly, plasma matrix contains abundant growth factors, including transforming growth factor, vascular endothelial growth factor, and platelet-derived growth factor, which can accelerate the healing of soft and hard tissues and reduce patient suffering. Plasma matrix also contains numerous leukocytes, which play an anti-inflammatory and anti-infective role, helping to prevent postoperative complications. Currently, plasma matrix preparation technology has evolved to utilize anticoagulant-free glass and plastic centrifuge tubes, allowing for the preparation of plasma matrix gel and concentrated plasma matrix liquid through a single centrifugation at a controlled speed.

[0003] Plasma matrix bone block preparation technology is a process based on plasma matrix gel and concentrated liquid, and involves the use of bone substitute materials such as Geistlich Bio- The combined use of bone powder produces a plasma matrix bone block with a certain degree of toughness, which combines the excellent biological properties of plasma matrix with the osteoconductive properties of bone substitute material, and can serve as a highly malleable regenerative scaffold material to accelerate osteogenesis.

[0004] However, as a crucial step in the preparation of plasma matrix bone blocks, data on its relationship with the surface modification of preparation tools remains insufficient. This leads to unstable forming time and relatively low mechanical strength of plasma matrix bone blocks in some clinical scenarios. Summary of the Invention

[0005] This invention aims to at least solve one of the technical problems existing in the prior art. To this end, one objective of this invention is to provide a plasma matrix bone block preparation component. By setting a first groove on a titanium mold with a surface roughness Rsm greater than or equal to 0.1 μm and less than or equal to 0.2 μm, this component promotes the formation of a fibrin network, accelerates the gel formation rate of the plasma matrix, enhances the cross-linking tightness between the plasma matrix and the bone substitute material, shortens the preparation time of the plasma matrix bone block, improves the preparation efficiency and mechanical strength of the plasma matrix bone block, and expands the clinical application scenarios of the plasma matrix bone block.

[0006] The present invention also proposes a method for producing a mold for producing the above-mentioned plasma matrix bone block preparation component;

[0007] The present invention also proposes a method for preparing plasma matrix bone blocks using the above-mentioned plasma matrix bone block preparation component.

[0008] A plasma matrix bone block preparation assembly according to a first aspect of the present invention includes: a first mold, the first mold having a first groove, the roughness Rsm of the inner surface of the first groove being greater than or equal to 0.1 μm and less than or equal to 0.2 μm; wherein the first mold is a titanium component.

[0009] The plasma matrix bone block preparation component provided by the present invention, by setting a first groove on a titanium mold with a surface roughness greater than or equal to 0.1 μm and less than or equal to 0.2 μm, can enhance the hydrophilicity of the inner surface of the first groove and make the inner surface of the first groove have good biocompatibility. When the bone-membrane mixed material is transferred into the first groove and liquid plasma matrix is ​​injected, platelets in the liquid plasma matrix can adhere and be activated, promote the formation of fibrin network, accelerate the formation rate of plasma matrix gel, enhance the cross-linking tightness between plasma matrix and bone substitute material, shorten the preparation time of plasma matrix bone blocks, improve the preparation efficiency and mechanical strength of plasma matrix bone blocks, and expand the clinical application scenarios of plasma matrix bone blocks.

[0010] According to some embodiments of the present invention, the roughness of the inner surface of the first groove is formed by immersing the surface in an acid etching reagent at 45°C-55°C for 50-70 minutes after polishing; wherein the acid etching reagent includes 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid.

[0011] A mold manufacturing method according to a second aspect of the present invention is used to produce a plasma matrix bone block preparation assembly according to the first aspect of the present invention. The mold manufacturing method includes:

[0012] Select a titanium sheet of a preset size;

[0013] A groove is cut into the titanium metal plate to form a first initial groove;

[0014] The inner surface of the first initial groove is polished sequentially to form the first intermediate groove;

[0015] The first intermediate tank is cleaned to remove fat-soluble and water-soluble impurities from the inner surface of the first intermediate tank.

[0016] The inner surface of the first intermediate tank is acid-etched to form the first tank.

[0017] According to the mold manufacturing method of the present invention, by grinding and acid etching the inner surface of the first initial groove, the roughness of the inner surface of the first groove can be modified to a preset range. At the same time, the oxide layer on the surface of the first groove can be effectively removed, and the hydrophilicity of the inner surface of the first groove can be improved. This allows platelets to adhere and be activated when they come into contact with the inner surface of the first groove, thereby accelerating the formation rate of plasma matrix gel, enhancing the cross-linking tightness between the plasma matrix and the bone substitute material, shortening the preparation time of plasma matrix bone blocks, improving the preparation efficiency and mechanical strength of plasma matrix bone blocks, and expanding the clinical application scenarios of plasma matrix bone blocks.

[0018] According to some embodiments of the present invention, the etching reagent used in acid etching the inner surface of the first intermediate tank includes 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid.

[0019] In some embodiments of the present invention, the acid etching method includes:

[0020] The acid etching reagent is heated to 50°C;

[0021] The heated acid etching reagent is injected into the first intermediate tank, and the inner surface of the first intermediate tank is completely submerged.

[0022] The inner surface of the first intermediate tank is immersed in the acid etching reagent for 60 minutes.

[0023] According to a third aspect embodiment of the present invention, a method for preparing plasma matrix bone blocks is used to prepare plasma matrix bone blocks, utilizing the plasma matrix bone block preparation components of the second aspect embodiment of the present invention described above. The method includes:

[0024] Collect fresh blood from the patient;

[0025] The patient's blood is centrifuged to obtain centrifuged blood products;

[0026] After centrifugation, the upper solid plasma matrix and liquid plasma matrix are collected.

[0027] The solid plasma matrix is ​​compressed into a plasma matrix membrane;

[0028] The plasma matrix membrane was cut into small pieces and mixed evenly with bone substitute material to obtain a bone-membrane hybrid material;

[0029] The bone-membrane hybrid material is transferred into the first tank;

[0030] The liquid plasma matrix is ​​injected onto the bone-membrane hybrid material, and after forming a gel state, a primary plasma matrix bone block is obtained.

[0031] Turn the primary plasma matrix bone block over to ensure that the primary plasma matrix bone block is in full contact with the inner surface of the first groove;

[0032] The liquid plasma matrix is ​​continuously injected into the first tank to completely submerge the primary plasma matrix bone block. After gel formation, the plasma matrix bone block is obtained.

[0033] According to the plasma matrix bone block preparation method provided by the present invention, by utilizing the plasma matrix bone block preparation component of the first aspect embodiment of the present invention, the preparation time of plasma matrix bone blocks can be shortened, the preparation efficiency and mechanical strength of plasma matrix bone blocks can be improved, and the clinical application scenarios of the prepared plasma matrix bone blocks can be expanded.

[0034] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0035] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0036] Figure 1 This is a top view of the plasma matrix bone block preparation assembly described in some embodiments of the present invention;

[0037] Figure 2 yes Figure 1 A cross-sectional view of the plasma matrix bone block preparation component;

[0038] Figure 3 yes Figure 1 Another cross-sectional view of the plasma matrix bone block preparation component;

[0039] Figure 4 A comparative diagram showing the effect of different roughness of the inner surface on the coagulation time of the plasma matrix;

[0040] Figure 5 A comparative diagram showing the effect of different inner surface roughness on the tensile fracture length of the plasma matrix membrane;

[0041] Figure 6 A comparative diagram showing the effect of different inner surface roughness on the tensile fracture strength of plasma matrix membrane;

[0042] Figure 7 Comparison of surface hydrophilicity of inner surfaces with different roughness after acid etching treatment;

[0043] Figure 8 Photographs showing the contact angle of a surface with a surface roughness Rsm = 0.9 mm after acid etching;

[0044] Figure 9Photographs showing the contact angle of a surface with a surface roughness Rsm = 0.15 mm after acid etching;

[0045] Figure 10 The image shows the contact angle of a surface with a surface roughness Rsm = 0.05 mm after acid etching.

[0046] Figure label:

[0047] 100. Plasma matrix bone block preparation component; 1. First tank. Detailed Implementation

[0048] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0049] The plasma matrix bone block preparation assembly 100 according to an embodiment of the present invention is described below with reference to the accompanying drawings.

[0050] Reference Figures 1-3 According to a first aspect embodiment of the present invention, a plasma matrix bone block preparation assembly 100 includes: a first mold, the first mold having a first groove 1, the roughness Rsm of the inner surface of the first groove 1 being greater than or equal to 0.1 μm and less than or equal to 0.2 μm; for example, the roughness Rsm of the inner surface of the first groove 1 can be 0.1 μm, 0.11 μm, 0.12 μm, 0.13 μm, 0.14 μm, 0.15 μm, 0.16 μm, 0.17 μm, 0.18 μm, 0.19 μm, or 0.2 μm. Specifically, grinding the inner surface of the first groove 1 can bring the roughness Rsm of the inner surface of the first groove 1 to a range greater than or equal to 0.1 μm and less than or equal to 0.2 μm.

[0051] Reference Figures 7-10 , Figure 7 Comparison of surface hydrophilicity of inner surfaces with different roughness after acid etching treatment; Figure 8 Photographs showing the contact angle of a surface with a surface roughness Rsm = 0.9 mm after acid etching; Figure 9 Photographs showing the contact angle of a surface with a surface roughness Rsm = 0.15 mm after acid etching; Figure 10 The image shows the contact angle of a surface with a surface roughness Rsm = 0.05 mm after acid etching. It can be seen that surfaces with surface roughness Rsm = 0.15 mm and Rsm = 0.05 mm, after acid etching, exhibit good hydrophilicity, allowing platelets to adsorb and activate, thus promoting the formation of fibrin networks.

[0052] By setting the roughness Rsm of the inner surface of the first groove 1 to be greater than or equal to 0.1 μm and less than or equal to 0.2 μm, the hydrophilicity of the inner surface of the first groove 1 can be enhanced, thereby promoting the formation of fibrin network.

[0053] The first mold is made of titanium. This allows the inner surface of the first groove 1 to have good biocompatibility. When liquid plasma matrix is ​​injected into the first groove 1, platelets are activated upon contact with the inner surface of the first groove 1, promoting the formation of fibrin network.

[0054] It is generally accepted in the art that a larger roughness corresponds to a larger contact area between the plasma matrix and the material, and therefore a faster gel formation rate. However, through extensive experiments conducted by the researchers of this invention, it was found that when the roughness Rsm is greater than or equal to 0.1 μm and less than or equal to 0.2 μm, the gel formation rate differs from the generally accepted rate and exhibits superior performance. (Refer to...) Figure 4 , Figure 4 This is a comparison chart showing the effect of different inner surface roughness on the coagulation time of the plasma matrix. Compared with the gel formation rate of the plasma matrix in a groove with a roughness of Rsm = 0.25 mm, the gel formation rate of the plasma matrix in a groove with a roughness of Rsm = 0.15 mm shows a more significant improvement; compared with the gel formation rate of the plasma matrix in a groove with a roughness of Rsm = 0.05 mm, the gel formation rate of the plasma matrix in a groove with a roughness of Rsm = 0.15 mm also shows a more significant improvement.

[0055] Based on the above findings, the researchers of this invention continued their experiments and discovered that plasma matrix membranes prepared by forming a gel on a surface with a roughness Rsm greater than or equal to 0.1 μm and less than or equal to 0.2 μm exhibit good tensile properties. Using this plasma matrix membrane, plasma matrix bone blocks with good mechanical properties can be prepared. (Refer to...) Figure 5 and Figure 6 , Figure 5 This is a comparison of the effects of different inner surface roughness on the tensile fracture length of the plasma matrix membrane. Figure 6 This is a comparison of the effects of different inner surface roughness on the tensile fracture strength of plasma matrix membranes. Among them, the plasma matrix membrane prepared with Rsm = 0.15 mm has the best tensile properties.

[0056] In summary, when preparing plasma matrix bone blocks, the bone-membrane mixture can be transferred to the first tank 1 first, and then liquid plasma matrix can be injected to promote the formation of fibrin network, accelerate the formation of plasma matrix gel, enhance the tightness of cross-linking between plasma matrix and bone substitute material, thereby improving the mechanical strength of plasma matrix bone blocks and expanding the clinical application scenarios of plasma matrix bone blocks.

[0057] According to the plasma matrix bone block preparation component 100 of the present invention, by setting a first groove 1 with a surface roughness greater than or equal to 0.1 μm and less than or equal to 0.2 μm on a titanium mold, the hydrophilicity of the inner surface of the first groove 1 can be enhanced, and the inner surface of the first groove 1 can have better biocompatibility. When the bone-membrane mixed material is transferred to the first groove 1 and liquid plasma matrix is ​​injected, platelets in the liquid plasma matrix can adhere and be activated on the inner surface of the first groove 1, promoting the formation of fibrin network, accelerating the formation rate of plasma matrix gel, enhancing the cross-linking tightness between plasma matrix and bone substitute material, shortening the preparation time of plasma matrix bone blocks, improving the preparation efficiency and mechanical strength of plasma matrix bone blocks, and expanding the clinical application scenarios of plasma matrix bone blocks.

[0058] According to some embodiments of the present invention, the roughness of the inner surface of the first groove 1 is formed by immersing the surface in an acid etching reagent at 45°C-55°C for 50-70 minutes after polishing; for example, the temperature of the acid etching reagent can be 45°C, 46°C, 47°C, 48°C, 49°C, 50°C, 51°C, 52°C, 53°C, 54°C, or 55°C. For example, the acid etching time can be 50 minutes, 51 minutes, 52 minutes, 53 minutes, 54 minutes, 55 minutes, 56 minutes, 57 minutes, 58 minutes, 59 minutes, 60 minutes, 61 minutes, 62 minutes, 63 minutes, 64 minutes, 65 minutes, 66 minutes, 67 minutes, 68 minutes, 69 minutes, or 70 minutes.

[0059] The etching reagent comprises 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid. For example, the nitric acid content in the etching reagent can be 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, or 12%; the hydrofluoric acid content can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, or 1.5%. For example, the ratio of nitric acid content to hydrofluoric acid content is greater than or equal to 5 and less than or equal to 15; specifically, the ratio of nitric acid content to hydrofluoric acid content can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15.

[0060] For example, the method of polishing the inner surface of the first initial groove includes: polishing the inner surface of the first initial groove in sequence with sandpaper of 80 grit, 120 grit, 180 grit, 240 grit, 320 grit, 400 grit, 1000 grit and 2500 grit, or etching the inner surface of the first initial groove with laser equipment.

[0061] Preferably, when acid etching is performed after the inner surface of the first tank 1 is polished, the content of nitric acid in the acid etching reagent is 10%, the content of hydrofluoric acid in the acid etching reagent is 1%, the temperature of the acid etching reagent is 50°C, and the acid etching time is 60 minutes.

[0062] By using the above-mentioned acid etching method to treat the inner surface of the polished first groove 1, the oxide layer formed on the inner surface of the first groove 1 can be effectively removed, forming small and dense pits, ensuring the hydrophilicity of the inner surface of the first groove 1, so that the inner surface of the first groove 1 can play the role of activating platelets.

[0063] According to a second aspect of the present invention, a mold manufacturing method is used to produce a plasma matrix bone block preparation assembly 100 according to the first aspect of the present invention. The mold manufacturing method includes:

[0064] Select a titanium sheet of a preset size;

[0065] A groove is cut into the titanium sheet to form the first initial groove;

[0066] The inner surface of the first initial groove is polished to form the first intermediate groove;

[0067] The first intermediate tank is cleaned to remove fat-soluble and water-soluble impurities from the inner surface of the first intermediate tank;

[0068] The inner surface of the first intermediate tank is acid-etched to form the first tank 1.

[0069] For example, after acid etching, the mold production method may also include: washing several times with deionized water and anhydrous ethanol alternately to remove residual acid etching reagents, and then drying the plasma matrix bone block preparation component 100 for later use.

[0070] For example, the method of polishing the inner surface of the first initial groove includes: polishing the inner surface of the first initial groove in sequence with sandpaper of 80 grit, 120 grit, 180 grit, 240 grit, 320 grit, 400 grit, 1000 grit and 2500 grit, or etching the inner surface of the first initial groove with laser equipment.

[0071] According to the mold production method of the present invention, by grinding and acid etching the inner surface of the first initial groove, the roughness of the inner surface of the first groove 1 can be modified to a preset range. At the same time, the oxide layer on the surface of the first groove 1 can be effectively removed, and the hydrophilicity of the inner surface of the first groove 1 can be improved, thereby enabling platelets to adhere and activate, accelerating the formation of plasma matrix gel, enhancing the cross-linking tightness between plasma matrix and bone substitute material, thereby improving the mechanical strength of plasma matrix bone blocks, shortening the preparation time of plasma matrix bone blocks, improving the preparation efficiency of plasma matrix bone blocks, and expanding the clinical application scenarios of plasma matrix bone blocks.

[0072] According to some embodiments of the present invention, the etching reagent used in acid etching the inner surface of the first intermediate tank includes 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid. For example, the content of nitric acid in the etching reagent can be 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, or 12%; for example, the content of hydrofluoric acid in the etching reagent can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, or 1.5%. For example, the ratio of the content of nitric acid to the content of hydrofluoric acid is greater than or equal to 5 and less than or equal to 15; specifically, the ratio of the content of nitric acid to the content of hydrofluoric acid can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15.

[0073] By treating the inner surface of the polished first groove 1 with the above-mentioned acid etching reagent, the oxide layer on the inner surface of the first groove 1 can be effectively removed, so that the inner surface of the first groove 1 can effectively activate platelets.

[0074] In some embodiments of the present invention, the acid etching method includes:

[0075] Heat the acid etching reagent to 45℃-55℃;

[0076] The heated acid etching reagent is injected into the first intermediate tank and completely submerged the inner surface of the first intermediate tank;

[0077] Immerse the inner surface of the first intermediate tank with an acid etching reagent for 50-70 minutes.

[0078] By using the above-mentioned acid etching method to treat the inner surface of the polished first tank 1, small and dense pits can be formed on the inner surface of the first tank 1, effectively ensuring the hydrophilicity of the inner surface of the first tank 1, so that the liquid plasma matrix can adhere and be activated on the inner surface of the first tank 1.

[0079] According to a third aspect embodiment of the present invention, a method for preparing plasma matrix bone blocks is used to prepare plasma matrix bone blocks, utilizing the plasma matrix bone block preparation assembly 100 of the second aspect embodiment of the present invention described above. The method includes:

[0080] Collect fresh blood from the patient;

[0081] The patient's blood is centrifuged to obtain centrifuged blood products;

[0082] After centrifugation, the upper solid plasma matrix and liquid plasma matrix are collected.

[0083] Compressing solid plasma matrix into a plasma matrix membrane;

[0084] The plasma matrix membrane was shredded and mixed evenly with bone substitute material to obtain a bone-membrane hybrid material;

[0085] The bone-membrane hybrid material is transferred into the first tank 1;

[0086] Liquid plasma matrix is ​​injected onto a bone-membrane hybrid material, and after gel formation, primary plasma matrix bone blocks are obtained.

[0087] Turn the primary plasma matrix bone block over to ensure that the primary plasma matrix bone block is in full contact with the inner surface of the first groove 1;

[0088] Liquid plasma matrix is ​​continuously injected into the first tank 1 to completely submerge the primary plasma matrix bone block. After it forms a gel state, that is, after it forms a gel, the plasma matrix bone block is obtained.

[0089] For example, when collecting fresh blood from a patient, the blood can be distributed from the patient's elbow crease into four glass blood collection tubes and two plastic blood collection tubes, ensuring each tube contains 10 ml of fresh blood. Then, the four glass and two plastic blood collection tubes containing fresh blood are immediately placed in a horizontal centrifuge and centrifuged at a relative centrifugal force of 550 g for 8 minutes. Afterward, the caps of the four glass blood collection tubes are opened, and the tubes are allowed to stand for 5 minutes until the plasma matrix gel in the glass tubes stabilizes. The plasma matrix gel is then removed with flat-tipped forceps and cut 1 mm below the red-yellow boundary. The yellow gel is placed in a perforated compression molding kit and compressed for 90 seconds to obtain four plasma matrix membranes. Simultaneously, the upper layer of liquid plasma matrix in the two plastic blood collection tubes is collected.

[0090] Subsequently, the two plasma matrix membranes were cut into pieces and gathered on one side of the first groove 1. On the other side of the first groove 1, an appropriate amount of bone replacement material was added according to the size of the patient's bone defect, and the plasma matrix membrane and bone replacement material were thoroughly mixed to obtain a bone-membrane mixture. Then, liquid plasma matrix was injected into the first groove 1 onto the bone-membrane mixture. The bone-membrane mixture was gently turned over to ensure that all surfaces of the bone-membrane mixture were in full contact with the inner surface of the treated first groove 1, so as to promote the formation of fibrin network and accelerate the formation of plasma matrix gel. After the gel was formed, a primary plasma matrix bone block was obtained.

[0091] After obtaining the primary plasma matrix bone block, liquid plasma matrix is ​​injected into the first tank 1, so that the liquid plasma matrix submerges the primary plasma matrix bone block. After standing for 3 minutes, a plasma matrix bone block with the same shape as the first tank 1 is obtained.

[0092] According to the plasma matrix bone block preparation method of the present invention, by utilizing the plasma matrix bone block preparation component 100 of the first aspect embodiment of the present invention, the preparation time of plasma matrix bone blocks can be shortened, the preparation efficiency of plasma matrix bone blocks can be improved, and the mechanical strength of plasma matrix bone blocks can be improved. At the same time, the prepared plasma matrix bone blocks can contain more platelets, leukocytes and cytokines, so that the prepared plasma matrix bone blocks have stronger osteogenic induction ability and expand the clinical application scenarios of the prepared plasma matrix bone blocks.

[0093] In the description of this invention, it should be understood that relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0094] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0095] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0096] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A plasma matrix bone block preparation assembly for preparing plasma matrix bone blocks, characterized in that, include: A first mold, wherein the first mold is provided with a first groove, and the surface roughness Rsm of the inner surface of the first groove is greater than or equal to 0.1 μm and less than or equal to 0.2 μm; The first mold is a titanium part.

2. The plasma matrix bone block preparation assembly according to claim 1, characterized in that, The roughness of the inner surface of the first tank is formed by immersing it in an acid etching reagent at 45℃-55℃ for 50min-70min after polishing. The etching reagent includes 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid.

3. A mold manufacturing method for producing a plasma matrix bone block preparation mold according to claim 1 or 2, characterized in that, include: Select a titanium sheet of a preset size; A groove is cut into the titanium metal plate to form a first initial groove; The inner surface of the first initial groove is polished to form the first intermediate groove; The first intermediate tank is cleaned to remove fat-soluble and water-soluble impurities from the inner surface of the first intermediate tank. The inner surface of the first intermediate tank is acid-etched to form the first tank.

4. The mold production method according to claim 3, characterized in that, The etching reagents used when etching the inner surface of the first intermediate tank include 8%-12% nitric acid and 0.5%-1.5% hydrofluoric acid.

5. The mold production method according to claim 4, characterized in that, The acid etching method includes: The acid etching reagent is heated to 45℃-55℃; The heated acid etching reagent is injected into the first intermediate tank, and the inner surface of the first intermediate tank is completely submerged. The inner surface of the first intermediate tank is immersed in the acid etching reagent for 50-70 minutes.

6. A method for preparing plasma matrix bone blocks, characterized in that, The method for preparing plasma matrix bone blocks using the plasma matrix bone block preparation assembly according to claim 1 or 2 includes: Collect fresh blood from the patient; The patient's blood is centrifuged to obtain centrifuged blood products; After centrifugation, the upper solid plasma matrix and liquid plasma matrix are collected. The solid plasma matrix is ​​compressed into a plasma matrix membrane; The plasma matrix membrane was cut into small pieces and mixed evenly with bone substitute material to obtain a bone-membrane hybrid material; The bone-membrane hybrid material is transferred into the first tank; The liquid plasma matrix is ​​injected onto the bone-membrane hybrid material, and after it forms a gel state, a primary plasma matrix bone block is obtained. Turn the primary plasma matrix bone block over to ensure that the primary plasma matrix bone block is in full contact with the inner surface of the first groove; The liquid plasma matrix is ​​continuously injected into the first tank to completely submerge the primary plasma matrix bone block. After gel formation, the plasma matrix bone block is obtained.

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