Method for synergistically extracting anti-saccharification composition from morchella esculenta and mulberry twigs
By synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches, the problem of insufficient anti-glycation effect in existing technologies has been solved, achieving full-chain inhibition of glycation reaction and improving the stability of active ingredients.
Patent Information
- Application Number
- CN202511477469.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-16
- Publication Date
- 2025-12-16
AI Technical Summary
In existing technologies, morel extracts have limited effects on the early and mid-to-late stages of glycation reactions, and extracts from a single source are insufficient to cover the entire glycation reaction chain, resulting in inadequate anti-glycation effects.
An anti-glycation composition was synergistically extracted from morel mushrooms and mulberry branches. Morel mushroom extract rich in polysaccharides and small molecule peptides was obtained through steps such as heating and reflux, ethanol precipitation, enzyme treatment, and ultrafiltration membrane concentration. Mulberry branch extract rich in flavonoids was obtained through steps such as ultrasound-assisted separation and freeze drying. Finally, the anti-glycation composition was formulated.
It achieves full-chain inhibition of glycation reaction, improves anti-glycation efficacy, ensures high purity and stability of active ingredients, and enhances the effects of early carbonyl capture, mid-term cross-linking inhibition, and late-stage AGEs removal.
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Figure CN121129938A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of natural product extraction, in particular to a method for synergistically extracting anti-glycation composition from morchella and mulberry branch. BACKGROUND
[0002] The non-enzymatic glycosylation of protein is a biochemical reaction commonly existing in the process of glucose metabolism disorder and body aging. In the reaction process, the carbonyl group of glucose, fructose and other reducing sugars can be combined with the amino group on the lysine or arginine residue in the protein molecule to form unstable Schiff base and Amadori product; then, these intermediates are further oxidized, dehydrated and cross-linked to gradually accumulate to form stable advanced glycation end products (AGEs), and the deposition of AGEs is closely related to diabetic complications, atherosclerosis, kidney function damage and skin collagen sclerosis; In the prior art, morchella extract is mainly rich in polysaccharides and small molecule peptides, which can capture carbonyl compounds in the early stage of glycation reaction, but has limited effect on the removal of middle-stage cross-linking reaction and late-stage AGEs; and mulberry branch extract is rich in flavonoids, which can inhibit the generation of AGEs through antioxidant and metal ion chelation in the middle and late stages, but lacks intervention on the early-stage carbonyl capture, and the single-source extract is difficult to cover the whole link of glycation reaction, resulting in insufficient overall anti-glycation effect; Therefore, a method for synergistically extracting anti-glycation composition from morchella and mulberry branch is provided. SUMMARY
[0003] Therefore, the present application provides a method for synergistically extracting anti-glycation composition from morchella and mulberry branch to solve or alleviate the technical problems in the prior art, and at least provides a beneficial choice.
[0004] The technical solution of the present application is as follows: a method for synergistically extracting anti-glycation composition from morchella and mulberry branch, comprising the following steps: S1, morchella extraction step: crushing dried or fresh morchella to a particle size of 40-80 meshes, adding deionized water for heating reflux extraction, the extraction temperature is 70-95 DEG C, the time is 1-3h, after filtering and removing the residue, the filtrate is subjected to ethanol precipitation, the ethanol precipitation adopts an ethanol solution with a volume fraction of 70%-85%, and the precipitation is collected after standing for 12-24h, and the content of impurities and cellulose is reduced by enzyme treatment, and the morchella extract rich in polysaccharides and small molecule peptides is obtained by concentrating through an ultrafiltration membrane with a molecular cut-off range of 3-10kDa; S2, mulberry branch extraction step: the mulberry branch is crushed to 20-60 mesh particle size, and an ethanol-water mixed solvent is added for ultrasonic-assisted extraction, the ethanol volume fraction in the mixed solvent is 50%-70%, the ultrasonic frequency is 20-40 kHz, and the time is 30-60 min, the extract is concentrated under reduced pressure, and then separated and purified by macroporous adsorption resin to obtain a mulberry branch extract rich in flavonoids; wherein the flavonoids include morusin, quercetin and derivatives thereof; S3, synergistic compounding step: the morchella extract and the mulberry branch extract are mixed according to a mass ratio of 1:1-1:5, a composition powder is prepared by spray drying or freeze drying, or the stability and solubility of the components are improved by cyclodextrin inclusion or nanoemulsification process to obtain an anti-glycation composition; S4, activity verification step: the effects of the composition on early carbonyl capture, middle cross-linking inhibition and late AGEs removal in the glycation reaction are detected by BSA-glucose system and methylglyoxal induction system in vitro, and verified by detecting the fluorescence intensity of AGEs in tissues or related biochemical indicators in animal experiments.
[0005] Further preferably, in the morchella extraction step, the ethanol precipitation uses an ethanol solution with a volume fraction of 75%-80%, the precipitation temperature is controlled at 4°C, and the precipitation time is 18 h.
[0006] Further preferably, the enzyme treatment is cellulase treatment, the addition amount of cellulase is 0.5%-1.5% of the substrate mass percentage, and the enzyme hydrolysis is carried out at 45-55°C for 1-2 h to destroy the cell wall structure and release polysaccharides.
[0007] Further preferably, the enzyme treatment is papain treatment, the addition amount of papain is 0.2%-0.8% of the substrate mass percentage, and the enzyme hydrolysis is carried out at 45-55°C for 1-2 h to remove residual proteins and increase the proportion of small molecule peptides.
[0008] Further preferably, the enzyme treatment is a combined treatment of cellulase and papain, wherein the mass ratio of cellulase to papain is 1:1-3:1, and the enzyme hydrolysis is carried out at 45-55°C for 1-2 h.
[0009] Further preferably, in the mulberry branch extraction step, the ethanol volume fraction in the ethanol-water mixed solvent is 60%, the solid-liquid ratio is 1:10-1:20 g / mL, and the ultrasonic power is 300-500 W.
[0010] Further preferably, the macroporous adsorption resin is AB-8 or D101 type, the adsorption time is 1-2 h, the elution solvent is an ethanol solution with a volume fraction of 30%-70%, and high-purity flavonoid components are obtained.
[0011] More preferably, in the synergistic compounding step, the mass ratio of morel extract to mulberry twig extract is 1:2 to 1:3.
[0012] More preferably, the compounded composition is obtained by spray drying with an inlet air temperature of 150-180°C, an outlet air temperature of 70-90°C, and an atomization pressure of 0.5-0.8 MPa to obtain a powdered formulation; or by freeze drying with a freezing temperature of -40--60°C, a vacuum degree of 20-50 Pa, and a drying time of 12-24 h.
[0013] More preferably, the synergistic compounding step includes cyclodextrin inclusion treatment, wherein the inclusion agent is hydroxypropyl-β-cyclodextrin, and the mass ratio of the compounded composition of morel extract and mulberry twig extract to the inclusion agent is 1:2 to 1:4.
[0014] The embodiments of the present invention have the following advantages due to the adoption of the above technical solutions: This invention utilizes the polysaccharides and small peptides in morel mushroom extract to competitively bind with carbonyl compounds via hydroxyl, amino, and other groups in the early stages of the reaction, thus blocking the initial glycation reaction. Meanwhile, flavonoids such as morin and quercetin in mulberry twig extract can scavenge free radicals and chelate transition metal ions in the mid-to-late stages, interrupting the oxidation chain reaction and slowing down the further aggregation and deposition of AGEs. This allows the two types of components to form a kinetic complementary relationship, reducing the oxidative inactivation of flavonoids while protecting the structural integrity of morel mushroom polysaccharides, thereby improving anti-glycation efficacy. Simultaneously, it ensures the high purity and stability of the active ingredients, avoiding the problems of insufficient formulation uniformity caused by polysaccharide degradation and flavonoid instability.
[0015] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description
[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0017] Figure 1 This is a flowchart of the steps of the present invention. Detailed Implementation
[0018] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.
[0019] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Example 1
[0020] This invention provides a method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches, comprising the following steps: S1, Morel mushroom extract Dried morel mushroom raw materials were selected, impurities were removed, and the powder was pulverized to a particle size of 60 mesh in a pulverizer to increase the specific surface area and improve the dissolution rate of the components. 100g of powder was weighed and added to 1500mL of deionized water (solid-liquid ratio of 1:15g / mL). The mixture was placed in a reflux device and heated at 85℃ for 2 hours. After extraction, the mixture was filtered with a filter cloth, the filtrate was collected, and insoluble residues were removed. The obtained filtrate was slowly added to a 78% ethanol solution while stirring, so that the ethanol volume fraction in the final solution was maintained at 78%. The solution was placed at 4℃ and allowed to stand for 18 hours to allow the polysaccharide to fully precipitate. The precipitate was collected by low-temperature centrifugation at 4000 r / min, and the supernatant was discarded. The precipitate was resuspended in deionized water and then treated with enzymes to reduce the content of impurities, proteins, and cellulose: an appropriate amount of compound enzyme preparation (including a small amount of protease and cellulase) was added to the solution and the solution was treated in a 50°C water bath for 1.5 h; after enzymatic hydrolysis, the enzyme was inactivated by heating at 95°C for 5 min; the resulting solution was concentrated and desalted by passing it through an ultrafiltration membrane with a molecular cutoff range of 5 kDa, and finally morel extract rich in polysaccharides and small molecule peptides was obtained. S2, Mulberry Twig Extract Fresh, mold-free mulberry branches were selected, cut, and dried in an oven at 60℃ to constant weight, then pulverized to a particle size of 40 mesh. 100g of powder was weighed and added to 1500mL of a 60% ethanol-water mixture with a solid-liquid ratio of 1:15g / mL. The mixture was placed in an ultrasonic extractor and treated for 45min at an ultrasonic frequency of 30kHz and an ultrasonic power of 400W. Ultrasonic action helps to break down plant cell walls and accelerate the dissolution of flavonoids. The extract was concentrated to 1 / 5 of its original volume using a rotary evaporator under reduced pressure, and then separated using an AB-8 macroporous adsorption resin column. The sample solution was loaded at a flow rate of 1 BV / h and the adsorption time was 1.5 h. Subsequently, it was washed with deionized water to remove impurities, and then eluted with 50% ethanol solution. The eluent was collected and concentrated under reduced pressure to obtain a high-purity flavonoid extract rich in morin, quercetin and its derivatives. S3, Synergistic Compounding The morel extract and mulberry twig extract prepared above were mixed at a mass ratio of 1:2, stirred evenly, and then dried by spray drying. The drying conditions were: inlet air temperature 160℃, outlet air temperature 80℃, and atomization pressure 0.6MPa. Spray drying can quickly remove solvent and obtain a powdered composition. The resulting product is an anti-glycation composition, which is a light brown powder with good flowability. In this embodiment, a cyclodextrin inclusion method can be used. The prepared composition powder is mixed with hydroxypropyl-β-cyclodextrin at a mass ratio of 1:3, dissolved in an appropriate amount of water, stirred for 30 minutes, and then freeze-dried to obtain the inclusion composition powder, which can effectively improve the water solubility and storage stability of flavonoids. Example 2
[0021] The steps in this embodiment are basically the same as those in Example 1, except for the morel mushroom enzyme treatment method and some process parameters: S1. Morel Extraction: Take dried morel powder (60 mesh size), add deionized water at a solid-liquid ratio of 1:12 g / mL, and extract by reflux at 90℃ for 1.5 h. After filtering to remove residue, add 80% ethanol solution and let stand at 4℃ for 18 h. Collect the precipitate, add cellulase at 1.0% of the substrate mass, and enzymatically hydrolyze at 50℃ for 1.5 h. After enzyme inactivation, concentrate through an ultrafiltration membrane with a molecular weight cutoff of 5 kDa to obtain morel polysaccharide extract.
[0022] S2. Mulberry Twig Extraction: Take mulberry twig powder with a particle size of 40 mesh, add it to a 55% (v / v) ethanol-water mixed solvent at a solid-liquid ratio of 1:12 g / mL, and ultrasonically extract for 40 min at an ultrasonic frequency of 25 kHz and an ultrasonic power of 350 W. After concentration under reduced pressure, the extract is adsorbed onto D101 macroporous adsorption resin for 1 h, and then eluted with a 40% (v / v) ethanol solution to obtain a flavonoid extract.
[0023] S3. Synergistic compounding: Morel mushroom extract and mulberry twig extract are mixed at a mass ratio of 1:3 and freeze-dried under the following conditions: freezing temperature -50℃, vacuum degree 30Pa, drying time 20h, to obtain a powdered composition. Example 3
[0024] The steps in this embodiment are basically the same as those in Example 1, except for the morel mushroom enzyme treatment method and some process parameters: S1. Morel Extraction: Take dried morel powder (70 mesh), add deionized water at a solid-liquid ratio of 1:18 g / mL, and reflux at 75℃ for 2.5 h. After filtration, add 72% (v / v) ethanol solution and let stand at 4℃ for 12 h. After collecting the precipitate, add papain at 0.5% (substrate mass percentage) and enzymatically hydrolyze at 50℃ for 1 h. After enzyme inactivation in a 95℃ water bath, concentrate through an ultrafiltration membrane with a molecular cutoff of 5 kDa to obtain the morel polysaccharide / peptide complex.
[0025] S2. Mulberry Twig Extraction: Take 50-mesh mulberry twig powder and add 70% (v / v) ethanol-water mixed solvent at a solid-liquid ratio of 1:18 g / mL. Extract for 50 min under ultrasonic conditions at a frequency of 35 kHz and an ultrasonic power of 450 W. After concentration under reduced pressure, adsorb the extract onto AB-8 resin for 2 h and elute with 60% (v / v) ethanol solution to obtain a flavonoid extract.
[0026] S3. Synergistic compounding: Morel mushroom extract and mulberry twig extract are mixed at a mass ratio of 1:1 and spray dried using a spray drying process with an inlet air temperature of 170℃, an outlet air temperature of 85℃, and an atomization pressure of 0.7MPa to obtain a light yellow powder composition. Example 4
[0027] This embodiment is basically the same as Example 1, except that the enzyme treatment of morel mushroom precipitate uses a combination of cellulase and papain, and some process parameters are also different. S1. Morel Extraction: Dried morel mushrooms were pulverized to 65 mesh and added to deionized water at a solid-liquid ratio of 1:16 g / mL. The mixture was refluxed at 80℃ for 2 hours. After filtration, a 76% (v / v) ethanol solution was added to the extract, and the mixture was allowed to stand at 4℃ for 18 hours to allow the polysaccharides to precipitate fully. After centrifugation, a compound enzyme preparation of cellulase and papain was added at a mass ratio of 2:1, with a total addition amount of 1.2% of the substrate mass. Enzymatic hydrolysis was performed at 50℃ for 1.5 hours. Subsequently, the enzyme was inactivated by heating at 95℃ for 5 minutes. The resulting solution was concentrated using an ultrafiltration membrane with a molecular cutoff of 5 kDa to obtain a morel extract rich in polysaccharides and small peptides.
[0028] S2. Mulberry Twig Extraction: Take 45-mesh mulberry twig powder and add 65% (v / v) ethanol-water mixed solvent at a solid-liquid ratio of 1:16 g / mL. Extract for 45 min under ultrasonic conditions at a frequency of 30 kHz and a power of 420 W. After concentrating the obtained extract under reduced pressure, adsorb it with D101 macroporous adsorption resin for 1.5 h and elute with 50% (v / v) ethanol solution to obtain flavonoid extract.
[0029] S3. Synergistic compounding: Morel extract and mulberry twig extract were mixed at a mass ratio of 1:3 and stirred evenly. The mixture was then freeze-dried under the following conditions: freezing temperature -55℃, vacuum degree 25Pa, and drying time 20h to obtain a light brown powdery anti-glycation composition.
[0030] Comparative Example 1: Water Extraction of Single Morel Mushroom Dried morel mushrooms were pulverized to 60 mesh and added to deionized water at a solid-liquid ratio of 1:15 g / mL. The mixture was heated and refluxed at 90 °C for 2 h. The extract was filtered and then directly concentrated without ethanol precipitation, enzyme treatment, or ultrafiltration separation to obtain crude morel extract.
[0031] Comparative Example 2: Single Mulberry Twig Ethanol Extraction Method The dried mulberry branches were crushed to 40 mesh and added to a 70% ethanol solution at a solid-liquid ratio of 1:10 g / mL. The mixture was soaked at room temperature for 24 h. The extract was concentrated under reduced pressure to obtain the mulberry branch ethanol extract directly, without the use of ultrasonic-assisted purification or resin purification.
[0032] Comparative Example 3: Direct Mixing Method of Morel Mushrooms and Mulberry Twigs The dried morel mushrooms were pulverized to 60 mesh, and the mulberry branches were pulverized to 40 mesh. They were then directly mixed in a 1:1 mass ratio without extraction, purification, compounding, or drying steps, resulting in a coarse mixed powder.
[0033] Experimental example: 1. The test sample includes Examples: The powder compositions obtained in Examples 1, 2, 3, and 4; Comparative examples: Comparative example 1 (crude water extract of Morus alba), Comparative example 2 (crude ethanol extract of Morus alba twigs), Comparative example 3 (direct mixture of Morus alba and Morus alba twigs).
[0034] 2. In vitro model and drug concentration 2.1. BSA-glucose model (early glycation) BSA: 10 mg / mL; glucose: 0.5 mol / L; PBS (pH 7.4) containing 0.02% NaN3; sample concentration: 0.50 mg / mL (based on total solids of the composition).
[0035] Incubate at 37℃ in the dark for 7 days; Detection: AGEs fluorescence intensity (Ex 370 nm / Em 440 nm).
[0036] 2.2. MGO-induced protein cross-linking model (mid-stage glycation) Gelatin solution: 2 mg / mL; MGO: 10 mmol / L; PBS (pH 7.4); Sample concentration: 0.25 mg / mL; Incubation at 37℃ for 24 h; Detection: Quantification of the relative strength of cross-linked bands by SDS-PAGE grayscale method; and recording of inhibition rate.
[0037] Calculation formula (consistent for both models): Inhibition rate (%) = 1 − (sample group − blank group) / (model group − blank group) × 100.
[0038] 2.3. Comparison Settings Blank group: Sugar-free / MGO-free; Model group: Contains sugar / MGO, no sample; Positive control: Acarbose (BSA-Glc, 0.5 mg / mL) / aminoguanidine (MGO, 0.25 mg / mL).
[0039] 3. In vivo model and dosing regimen (late-stage glycation) Animals: C57BL / 6 mice, male, 8 mice / group.
[0040] Model establishment: high sugar and high fat feeding + intraperitoneal injection of MGO (40 mg / kg, once every other day, for a total of 2 weeks).
[0041] Administration: Each sample was administered 100 mg / kg·d by gavage for 4 consecutive weeks; the model group was administered an equal volume of distilled water by gavage.
[0042] Endpoint Indicator: Fluorescence intensity of AGEs in kidney tissue (Ex 370 nm / Em 440 nm, normalized to protein content); Protein carbonyl content (DNPH method, nmol / mg protein).
[0043] Statistics: mean ± standard deviation, one-way ANOVA, Dunnett post-hoc test, p < 0.05 marked with "*".
[0044] II. Results and Data Table 1: Comparison of in vitro anti-glycation activities (all n=3, mean ± SD)
[0045] In summary: the inhibition rate relative to the model group; *: significant difference compared to Example 1 (p<0.05).
[0046] Early model (BSA–Glc): Example 2, Example 4 > Example 1 > Example 3; Cellulase / complex enzymes significantly enhance "polysaccharide release + carbonyl capture".
[0047] Mid-term model (MGO): Examples 3 and 4 showed the best performance, indicating that "increasing the proportion of small peptides through protein hydrolysis" is beneficial for inhibiting mid-term cross-linking; the complex enzyme takes into account both ends.
[0048] The proportions of each pair are lower than those of the present invention: single morel mushroom is only slightly effective in the early stage; single mulberry branch is slightly effective in the middle stage; direct mixing of powders is almost ineffective.
[0049] Table 2: Comparison of late-stage in vivo indicators (n=8, mean ± SD)
[0050] Summary: Percentage reduction compared to the model group; *: Significant difference compared to Example 1 (p < 0.05).
[0051] Example 4 (complex enzyme) was significantly superior to other examples in both late-stage indicators.
[0052] Examples 2 and 3 focus on "early carbonyl capture enhancement" and "mid-term crosslinking inhibition enhancement," respectively, and also demonstrate complementary division of labor in vivo.
[0053] The overall effect of the ratio is weak, and direct mixing of powders yields almost no anti-glycation benefit.
[0054] III. Experimental Conclusions The composition prepared by the method of the present invention is significantly superior to the conventional method in both the early BSA-Glc and mid-term MGO in vitro models. The complex enzyme treatment (Example 4) takes into account both ends of the process and achieves a comprehensive level close to that of the positive control.
[0055] In terms of late-stage in vivo indicators (AGEs fluorescence and protein carbonyl groups), Examples 1–4 were significantly superior to the comparative examples, and Example 4 > Example 2 ≈ Example 3 > Example 1, consistent with the complementary division of labor in vitro.
[0056] Overall, the data demonstrate that this invention achieves a full-chain anti-glycation process—from early carbonyl capture to mid-term cross-linking inhibition to late-term AGEs clearance—through the synergistic combination of "morel polysaccharide / small molecule peptide × mulberry flavonoids" and the integration of enzyme treatment, resin purification, and drying / inclusion stabilization. Compared to single-source or crude processes, the overall inhibitory effect is improved, and the stability and uniformity of the formulation are also enhanced simultaneously.
[0057] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in the present invention, and these should all be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.
Claims
1. A method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches, characterized in that: Includes the following steps: S1. Morel Extraction Steps: Crush dried or fresh morels to a particle size of 40-80 mesh, add deionized water and heat under reflux for extraction at a temperature of 70-95℃ for 1-3 hours. After filtration to remove residue, precipitate the filtrate with ethanol using a 70%-85% (v / v) ethanol solution. Let stand for 12-24 hours, collect the precipitate, treat it with enzymes to reduce the content of impurities and cellulose, and concentrate it through an ultrafiltration membrane with a molecular cutoff range of 3-10 kDa to obtain a morel extract rich in polysaccharides and small molecule peptides. S2. Mulberry twig extraction steps: Mulberry twigs are crushed to a particle size of 20-60 mesh, and ultrasonic-assisted extraction is carried out by adding an ethanol-water mixed solvent. The volume fraction of ethanol in the mixed solvent is 50%-70%, the ultrasonic frequency is 20-40kHz, and the time is 30-60min. After the extract is concentrated under reduced pressure, it is separated and purified by macroporous adsorption resin to obtain mulberry twig extract rich in flavonoids. The flavonoids mentioned therein include morin, quercetin and their derivatives; S3, Synergistic compounding step: The morel extract and the mulberry twig extract are mixed at a mass ratio of 1:1 to 1:5, and the composition powder is prepared by spray drying or freeze drying, or the stability and solubility of the components are improved by cyclodextrin inclusion or nano-emulsification process to obtain an anti-glycation composition. S4. Activity verification steps: The effects of the anti-glycation composition on early carbonyl capture, mid-term crosslinking inhibition and late-stage AGEs clearance in the in vitro BSA-glucose system and methylglyoxal induction system were detected respectively. The activity was verified by detecting the fluorescence intensity of AGEs in tissues or related biochemical indicators in animal experiments.
2. The method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, In the morel mushroom extraction step, ethanol precipitation is performed using an ethanol solution with a volume fraction of 75%–80%, the precipitation temperature is controlled at 4°C, and the precipitation time is 18 hours.
3. The method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The enzyme treatment is cellulase treatment, with the amount of cellulase added being 0.5% to 1.5% of the substrate mass percentage. Enzymatic hydrolysis is carried out at 45 to 55°C for 1 to 2 hours to destroy the cell wall structure and release polysaccharides.
4. The method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The enzyme treatment is papain treatment, with the amount of papain added being 0.2% to 0.8% of the substrate mass percentage. Enzymatic hydrolysis is carried out at 45 to 55°C for 1 to 2 hours to remove residual protein and increase the proportion of small molecule peptides.
5. A method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The enzyme treatment is a combined treatment of cellulase and papain, wherein the mass ratio of cellulase to papain is 1:1 to 3:1, and the enzymatic hydrolysis is carried out at 45 to 55°C for 1 to 2 hours.
6. The method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, In the mulberry branch extraction step, the volume fraction of ethanol in the ethanol-water mixed solvent is 60%, the solid-liquid ratio is 1:10 to 1:20 g / mL, and the ultrasonic power is 300 to 500 W.
7. The method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The macroporous adsorption resin is AB-8 or D101 type, the adsorption time is 1 to 2 hours, and the elution solvent is an ethanol solution with a volume fraction of 30% to 70% to obtain high-purity flavonoid components.
8. A method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, In the synergistic compounding step, the mass ratio of morel extract to mulberry twig extract is 1:2 to 1:
3.
9. A method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The synergistic compound is spray-dried at an inlet air temperature of 150–180°C, an outlet air temperature of 70–90°C, and an atomization pressure of 0.5–0.8 MPa to obtain a powdered formulation; or it is freeze-dried at a freezing temperature of -40–-60°C, a vacuum degree of 20–50 Pa, and a drying time of 12–24 h.
10. A method for synergistically extracting an anti-glycation composition from morel mushrooms and mulberry branches according to claim 1, characterized in that, The synergistic compounding step includes cyclodextrin inclusion treatment, wherein the inclusion agent is hydroxypropyl-β-cyclodextrin, and the mass ratio of the compounded composition of morel extract and mulberry twig extract to the inclusion agent is 1:2 to 1:4.