A composition containing ginseng extract and its preparation method

Abstract

This invention relates to the field of ginseng extract technology, specifically to a composition containing ginseng extract and its preparation method. The provided composition containing ginseng extract comprises the following substances in parts by weight: 5-30 parts of a ginseng peptide composition with a molecular weight less than 3000 Da; 1-15 parts of a saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2; 3-10 parts of a ginseng polysaccharide-selenium nanocrystal composition; 1-5 parts of *Lactobacillus plantarum* P-8 lyophilized powder; 0.5-3 parts of sodium tanshinone; 3-20 parts of a prebiotic composition; and 0.1-5 parts of a vitamin composition. A method for preparing the aforementioned ginseng extract composition is also provided. The prepared ginseng extract composition exhibits excellent properties and has good application prospects.

Description

Technical Field

[0001] This invention relates to the field of ginseng extract technology, specifically to a composition containing ginseng extract and its preparation method. Background Technology

[0002] Ginseng, a traditional and precious Chinese medicinal herb, has a long history of application. Modern pharmacological studies have shown that ginseng contains a variety of active ingredients, including ginsenosides, ginseng polysaccharides, and ginseng peptides, which have various physiological activities such as enhancing immunity, improving cognitive function, anti-fatigue, and anti-oxidation.

[0003] The current research and application of ginseng extracts have the following limitations:

[0004] Firstly, traditional ginseng extracts mainly focus on ginsenosides, neglecting the research and application of ginseng peptides. Ginseng peptides are small molecular fragments produced by the enzymatic hydrolysis of ginseng proteins, possessing advantages such as small molecular weight, easy absorption, and high bioavailability. Studies have shown that ginseng peptides have effects such as protecting intestinal health and enhancing immunity; however, current technologies are not perfect in their preparation processes and activity protection, resulting in low bioactivity of the products.

[0005] Secondly, the synergistic effects of the components in existing ginseng products have not been fully explored. Ginseng peptide complexes have significant immunomodulatory functions, but optimizing their composition ratio and preparation process to maximize bioactivity remains a technical challenge.

[0006] Third, the extraction methods and efficiency of ginseng active ingredients in existing technologies need to be improved. For example, traditional ginseng peptide preparation methods usually use single enzymatic hydrolysis, which has low extraction efficiency and makes it difficult to obtain peptides with specific active sequences.

[0007] In addition, existing products containing ginseng extract have relatively simple formulation designs and lack systematic consideration of the synergistic effects between the components.

[0008] Therefore, developing a composition containing multiple active ingredients such as ginsenosides and ginsenosides, and fully leveraging the synergistic effects of each component through optimized preparation process and formulation design, has significant technological innovation value and market application prospects.

[0009] CN119366629A discloses a ginseng and nattokinase composition and its preparation method, belonging to the field of microbial fermentation technology. The provided ginseng and nattokinase composition includes ginsenosides, ginseng peptides, acid-soluble proteins, stabilizers, adjuvants, and ginseng itself. The preparation method includes: S1: washing, crushing, and steaming the ginseng, cooling it to 25-36℃ and letting it stand for 24 hours to obtain steamed ginseng; S2: adding adjuvants and stabilizers to the steamed ginseng from step S1, then inoculating with natto bacteria, and culturing at 32-35℃ for 24-36 hours to obtain a fermentation broth; S3: adding adjuvants again to the fermentation broth from step S2, mixing evenly, culturing at 36-40℃ for 24-48 hours, and then drying at low temperature to obtain the ginseng and nattokinase composition. The preparation method is simple and easy to promote.

[0010] However, the synergistic effect of its active components is not obvious. Therefore, developing a composition containing multiple active ingredients such as ginsenosides and ginsenosides, and fully leveraging the synergistic effect of each component through optimized preparation process and formulation design, has significant technological innovation value and market application prospects. Summary of the Invention

[0011] The purpose of this invention is to provide a composition containing ginseng extract, comprising the following substances in parts by weight:

[0012] 5-30 parts of ginseng peptide composition with a molecular weight of less than 3000 Da

[0013] 1-15 parts of a saponin composition consisting of rare ginsenosides Rg3, Rg5, and Rh2

[0014] 3-10 parts of ginseng polysaccharide-selenium nanocrystal composition

[0015] 1-5 parts of Lactobacillus plantarum P-8 lyophilized powder

[0016] Sodium tanshinone 0.5-3 parts

[0017] 3-20 parts of prebiotic composition

[0018] Vitamin composition 0.1-5 parts.

[0019] The sodium tanshinone component in the composition is expected to form a cross-component synergistic effect with ginseng peptides in promoting neuroprotection, based on its synergistic effect with ginseng peptides in promoting neuroprotection.

[0020] Furthermore, the preparation method of the ginseng peptide composition is as follows:

[0021] S11. Raw material pretreatment: Take five-year-old fresh ginseng, wash it, and then use liquid nitrogen quick-freezing technology to treat it at -196℃ for 30 minutes, followed by rapid thawing. This freeze-thaw cycle can destroy cell structure and improve enzymatic hydrolysis efficiency.

[0022] S12. Compound enzymatic hydrolysis: Adjust the pH of the pretreated ginseng slurry to 7.5, add V8 protease at 2% of the substrate mass, and enzymatically hydrolyze at 45℃ and normal pressure for 2 hours;

[0023] S13. High hydrostatic pressure-assisted enzymatic hydrolysis: Adjust the pH to 7.0, add aminopeptidase at 1% of the substrate mass, place the mixture in a high hydrostatic pressure reactor, and use staged pressure control. Apply 100 MPa for the first 2 hours and increase it to 200 MPa for the next 2 hours. Maintain the temperature at 50℃.

[0024] S14. Peptide separation and purification: After inactivating the enzyme in the solution obtained from the enzymatic hydrolysis in step S13, centrifuge to collect the supernatant. Pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and spray dry to obtain a ginseng peptide composition with a molecular weight of less than 3000 Da. Pack it in nitrogen for later use.

[0025] Ginseng peptides are small molecule peptides extracted from ginseng protein through enzymatic hydrolysis. They have multiple functions such as protecting the intestinal barrier, regulating immunity, and antioxidation. The preparation method of this invention produces ginseng peptides with good yield, small molecular weight, and easy absorption.

[0026] Furthermore, the preparation method of the saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2 is as follows:

[0027] S21. Raw material extraction: Take dried ginseng root, crush it, add 70% ethanol, and reflux at 80℃ for 3 times, 2 hours each time. Combine the extracts.

[0028] S22. Preliminary purification: The extract is concentrated under reduced pressure until there is no alcohol odor, and then passed through a macroporous adsorption resin column. First, impurities are removed by elution with water, and then 50% ethanol is used for elution. The eluent is collected.

[0029] S23. Probiotic conversion: Concentrate the above eluent to a ginsenoside concentration of 100 mg / mL, and add Lactobacillus plantarum P-8 bacterial suspension (bacterial count ≥ 1 × 10⁻⁶) at a volume ratio of 10:1. 9 (CFU / mL), fermented under anaerobic conditions at 37°C for 60 hours;

[0030] S24. Purification of conversion products: The supernatant was obtained by centrifugation of the fermentation broth and separated by high efficiency preparative liquid chromatography to collect the Rg3, Rg5 and Rh2 fractions.

[0031] Ginsenosides are one of the main active ingredients of ginseng. This invention particularly selects rare ginsenosides Rg3, Rg5 and Rh2. These rare ginsenosides have stronger biological activity and absorption rate. Ginsenosides Rg3 and Rg5 have significant effects in treating or preventing neurological function decline, while ginsenoside Rh2 has the effect of repairing DNA and reducing cell apoptosis.

[0032] Furthermore, the preparation method of the ginseng polysaccharide-selenium nanocrystal composition is as follows:

[0033] S31. Ginseng polysaccharide extraction: Take the residue after ginseng extraction, add 90℃ hot water to extract 3 times, 2 hours each time, combine the extracts, concentrate and precipitate with alcohol, collect the precipitate to obtain ginseng polysaccharide;

[0034] S32. Preparation of nanocrystals: Ginseng polysaccharide and sodium selenite were dissolved in deionized water at a mass ratio of 8:1 to prepare a 5% concentration solution. Vitamin C at 0.2% of the mass of ginseng polysaccharide was added as a reducing agent. The mixture was reacted in a water bath at 70°C for 3 hours. After filtration and drying, a ginseng polysaccharide-selenium nanocrystal composition was obtained.

[0035] This invention innovatively develops a ginseng polysaccharide-selenium nanocrystal carrier system, which not only solves the solubility problem of saponins and peptides, but also introduces the synergistic antioxidant function of selenium, forming a unique delivery system.

[0036] Furthermore, the prebiotic composition is a mixture of xylooligosaccharides, galactooligosaccharides, and resistant dextrin in a mass ratio of 1:1:2. The prebiotics used in this invention include xylooligosaccharides, galactooligosaccharides, and resistant dextrin. These functional oligosaccharides can directly reach the large intestine to selectively promote the proliferation of probiotics, thereby regulating immunity and intestinal function. Prebiotics and ginseng peptides have a synergistic effect, promoting the absorption and utilization of ginseng peptides, while ginseng peptides can also enhance the probiotic effects of prebiotics, forming a bidirectional regulatory mechanism.

[0037] Further, the vitamin composition is a mixture of vitamin C, vitamin D, and vitamin E in a mass ratio of 10:0.005:2. Vitamin C, vitamin D, and vitamin E are particularly preferred in this invention. These micronutrients participate as cofactors in various metabolic processes in the human body. For example, vitamin C is an important antioxidant component that can enhance the antioxidant activity of ginseng peptides and ginsenosides, while B vitamins participate in energy metabolism and synergistically work with ginseng's anti-fatigue function.

[0038] The components in the composition of the present invention exhibit significant synergistic effects, mainly in the following aspects:

[0039] Gut-brain axis synergy: Ginseng peptides maintain gut health by improving intestinal barrier function, promoting intestinal mucus secretion, and enhancing the antioxidant capacity of intestinal cells; while ginsenosides act directly on the central nervous system, improving neurological function decline. Through the bidirectional communication pathway of the "gut-brain axis," both achieve systemic regulation from the gut to the brain, producing a synergistic effect.

[0040] Synergistic Immunomodulation: Studies on ginseng peptide complexes have shown that low molecular weight ginseng peptides can significantly improve cell activity, while ginsenosides also have immunomodulatory functions. The combination of the two can simultaneously regulate cellular immunity and humoral immunity, producing a more comprehensive immune enhancement effect.

[0041] Synergistic antioxidant effects: Ginseng peptides enhance the antioxidant capacity of intestinal cells, while vitamin C and vitamin E are also important antioxidant components. The combination of the two can form a stronger antioxidant defense network, scavenging various free radicals and protecting cells from oxidative damage.

[0042] Synergistic absorption and utilization: Prebiotics improve gut microbiota, produce short-chain fatty acids, promote gut health, and thus enhance the absorption and bioavailability of ginseng peptides and ginsenosides. Studies have shown that a good gut environment can significantly improve the bioavailability of active ingredients.

[0043] The present invention also provides a method for preparing the above-mentioned ginseng extract composition, the process of which is as follows: a rare ginsenoside and ginseng peptide composition is mixed with a ginseng polysaccharide-selenium nanocrystal composition solution in a certain proportion, and loaded under gentle stirring for 24 hours to form a carrier system. Then, other components are added in a certain proportion and mixed evenly. Granules are prepared by wet granulation process. After drying, lubricants such as magnesium stearate are added and the mixture is compressed into tablets or filled into enteric capsules. Enteric capsules can ensure that the composition is released in a specific part of the intestine, maximizing the survival rate of probiotics and the conversion efficiency of saponins.

[0044] The composition containing ginseng extract and its preparation method provided by the present invention have the following significant advantages:

[0045] First, synergistic effects through multiple mechanisms. The ginseng peptides and ginsenosides in this composition exert synergistic effects through different mechanisms of action. Ginseng peptides mainly act on intestinal barrier function and immune regulation, while ginsenosides focus on neuroprotection and cognitive improvement. The combination of the two can achieve bidirectional regulation of the "gut-brain axis" and produce synergistic effects.

[0046] Secondly, it has high bioavailability. The ginseng peptides prepared through complex enzymatic hydrolysis and high hydrostatic pressure technology have small molecular weights, are easily absorbed, and retain specific bioactive sequences. Simultaneously, the addition of the prebiotic composition further promotes the absorption and utilization of active ingredients in the intestines.

[0047] Third, multiple physiological functions. This composition not only improves cognitive function, but also enhances immunity, combats fatigue, and protects gut health, meeting the diverse needs of modern consumers for health products.

[0048] Fourth, the preparation process is advanced. The composite enzymatic hydrolysis-high hydrostatic pressure synergistic extraction technology used in this invention improves extraction efficiency and better preserves the biological functions of active ingredients compared to traditional single enzymatic hydrolysis processes. Detailed Implementation

[0049] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0050] Example 1: A composition containing ginseng extract, comprising the following substances:

[0051] 20g of ginseng peptide composition with a molecular weight of less than 3000 Da

[0052] 2g of a saponin composition consisting of rare ginsenosides Rg3, Rg5, and Rh2

[0053] 5g of ginseng polysaccharide-selenium nanocrystal composition

[0054] 2g of Lactobacillus plantarum P-8 lyophilized powder

[0055] 1g of tanshinone sodium

[0056] 5g of prebiotic composition

[0057] Vitamin composition 1g.

[0058] The preparation method of the ginseng peptide composition is as follows:

[0059] S11. Raw material pretreatment: Take five-year-old fresh ginseng, wash it, and then treat it at -196℃ for 30 minutes using liquid nitrogen quick-freezing technology, followed by rapid thawing;

[0060] S12. Compound enzymatic hydrolysis: Adjust the pH of the pretreated ginseng slurry to 7.5, add V8 protease at 2% of the substrate mass, and enzymatically hydrolyze at 45℃ and normal pressure for 2 hours;

[0061] S13. High hydrostatic pressure-assisted enzymatic hydrolysis: Adjust the pH to 7.0, add aminopeptidase at 1% of the substrate mass, place the mixture in a high hydrostatic pressure reactor, and use staged pressure control. Apply 100 MPa for the first 2 hours and increase it to 200 MPa for the next 2 hours. Maintain the temperature at 50℃.

[0062] S14. Peptide separation and purification: After inactivating the enzyme in the solution obtained from the enzymatic hydrolysis in step S13, centrifuge to collect the supernatant. Pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and spray dry to obtain a ginseng peptide composition with a molecular weight of less than 3000 Da. Pack it in nitrogen for later use.

[0063] The preparation method of the saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2 is as follows:

[0064] S21. Raw material extraction: Take dried ginseng root, crush it, add 70% ethanol, and reflux at 80℃ for 3 times, 2 hours each time. Combine the extracts.

[0065] S22. Preliminary purification: The extract is concentrated under reduced pressure until there is no alcohol odor, and then passed through a macroporous adsorption resin column. First, impurities are removed by elution with water, and then 50% ethanol is used for elution. The eluent is collected.

[0066] S23. Probiotic conversion: Concentrate the above eluent to a ginsenoside concentration of 100 mg / mL, and add Lactobacillus plantarum P-8 bacterial suspension (bacterial count ≥ 1 × 10⁻⁶) at a volume ratio of 10:1. 9 (CFU / mL), fermented under anaerobic conditions at 37°C for 60 hours;

[0067] S24. Purification of conversion products: The supernatant was obtained by centrifugation of the fermentation broth and separated by high efficiency preparative liquid chromatography to collect the Rg3, Rg5 and Rh2 fractions.

[0068] The preparation method of the ginseng polysaccharide-selenium nanocrystal composition is as follows:

[0069] S31. Ginseng polysaccharide extraction: Take the residue after ginseng extraction, add 90℃ hot water to extract 3 times, 2 hours each time, combine the extracts, concentrate and precipitate with alcohol, collect the precipitate to obtain ginseng polysaccharide;

[0070] S32. Preparation of nanocrystals: Ginseng polysaccharide and sodium selenite were dissolved in deionized water at a mass ratio of 8:1 to prepare a 5% concentration solution. Vitamin C at 0.2% of the mass of ginseng polysaccharide was added as a reducing agent. The mixture was reacted in a water bath at 70°C for 3 hours. After filtration and drying, a ginseng polysaccharide-selenium nanocrystal composition was obtained.

[0071] The prebiotic composition is a mixture of xylooligosaccharides, galactooligosaccharides, and resistant dextrin in a mass ratio of 1:1:2.

[0072] The vitamin composition is a mixture of vitamin C, vitamin D and vitamin E in a mass ratio of 10:0.005:2.

[0073] Example 2: A composition containing ginseng extract, comprising the following substances:

[0074] 25g of ginseng peptide composition with a molecular weight of less than 3000 Da

[0075] 4g of a saponin composition consisting of rare ginsenosides Rg3, Rg5, and Rh2

[0076] 6g of ginseng polysaccharide-selenium nanocrystal composition

[0077] Lactobacillus plantarum P-8 lyophilized powder 3 g

[0078] 1g of tanshinone sodium

[0079] 8g of prebiotic composition

[0080] Vitamin composition 2g.

[0081] The preparation method of the ginseng peptide composition is as follows:

[0082] S11. Raw material pretreatment: Take five-year-old fresh ginseng, wash it, and then treat it at -196℃ for 30 minutes using liquid nitrogen quick-freezing technology, followed by rapid thawing;

[0083] S12. Compound enzymatic hydrolysis: Adjust the pH of the pretreated ginseng slurry to 7.5, add V8 protease at 2% of the substrate mass, and enzymatically hydrolyze at 45℃ and normal pressure for 2 hours;

[0084] S13. High hydrostatic pressure-assisted enzymatic hydrolysis: Adjust the pH to 7.0, add aminopeptidase at 1% of the substrate mass, place the mixture in a high hydrostatic pressure reactor, and use staged pressure control. Apply 100 MPa for the first 2 hours and increase it to 200 MPa for the next 2 hours. Maintain the temperature at 50℃.

[0085] S14. Peptide separation and purification: After inactivating the enzyme in the solution obtained from the enzymatic hydrolysis in step S13, centrifuge to collect the supernatant. Pass the supernatant through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, and spray dry to obtain a ginseng peptide composition with a molecular weight of less than 3000 Da. Pack it in nitrogen for later use.

[0086] The preparation method of the saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2 is as follows:

[0087] S21. Raw material extraction: Take dried ginseng root, crush it, add 70% ethanol, and reflux at 80℃ for 3 times, 2 hours each time. Combine the extracts.

[0088] S22. Preliminary purification: The extract is concentrated under reduced pressure until there is no alcohol odor, and then passed through a macroporous adsorption resin column. First, impurities are removed by elution with water, and then 50% ethanol is used for elution. The eluent is collected.

[0089] S23. Probiotic conversion: Concentrate the above eluent to a ginsenoside concentration of 100 mg / mL, and add Lactobacillus plantarum P-8 bacterial suspension (bacterial count ≥ 1 × 10⁻⁶) at a volume ratio of 10:1. 9 (CFU / mL), fermented under anaerobic conditions at 37°C for 60 hours;

[0090] S24. Purification of conversion products: The supernatant was obtained by centrifugation of the fermentation broth and separated by high efficiency preparative liquid chromatography to collect the Rg3, Rg5 and Rh2 fractions.

[0091] The preparation method of the ginseng polysaccharide-selenium nanocrystal composition is as follows:

[0092] S31. Ginseng polysaccharide extraction: Take the residue after ginseng extraction, add 90℃ hot water to extract 3 times, 2 hours each time, combine the extracts, concentrate and precipitate with alcohol, collect the precipitate to obtain ginseng polysaccharide;

[0093] S32. Preparation of nanocrystals: Ginseng polysaccharide and sodium selenite were dissolved in deionized water at a mass ratio of 8:1 to prepare a 5% concentration solution. Vitamin C at 0.2% of the mass of ginseng polysaccharide was added as a reducing agent. The mixture was reacted in a water bath at 70°C for 3 hours. After filtration and drying, a ginseng polysaccharide-selenium nanocrystal composition was obtained.

[0094] The prebiotic composition is a mixture of xylooligosaccharides, galactooligosaccharides, and resistant dextrin in a mass ratio of 1:1:2.

[0095] The vitamin composition is a mixture of vitamin C, vitamin D and vitamin E in a mass ratio of 10:0.005:2.

[0096] Comparative Example 1: The ginseng peptide composition component in Example 1 was removed, and the rest was the same as in Example 1, so it will not be repeated here.

[0097] Comparative Example 2: The ginsenoside composition component in Example 1 was removed, and the rest was the same as in Example 1, so it will not be repeated here.

[0098] Comparative Example 3: The prebiotic composition component in Example 1 was removed, and the rest was the same as in Example 1, so it will not be repeated here.

[0099] The technical effects of the compositions containing ginseng extract prepared in the above embodiments and comparative examples were investigated in the following experiments 1, 2 and 3. The test results are shown in Tables 1, 2 and 3.

[0100] Experiment 1: Memory Ability Test

[0101] The Morris water maze test was used to assess the spatial learning and memory abilities of mice. Sixty mice were randomly divided into six groups: a blank control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with ten mice in each group. Each group was administered the corresponding sample by gavage at a dose of 500 mg / kg / day for 30 consecutive days. One hour after the last administration, the water maze test was performed, and the escape latency and the number of times the mice crossed platforms were recorded.

[0102] Experiment 2: Immune Regulation Function Test

[0103] A cyclophosphamide-induced immunosuppressed mouse model was used to determine the proliferation capacity of spleen cells and the activity of NK cells. Sixty mice were randomly divided into six groups: a blank control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 10 mice in each group. Except for the blank control group, the other groups were intraperitoneally injected with cyclophosphamide 100 mg / kg to establish the immunosuppressed model. The corresponding samples were administered by gavage at a dose of 500 mg / kg / day for 14 consecutive days. Spleen cell proliferation capacity and NK cell activity were measured after the last administration.

[0104] Experiment 3: Anti-fatigue effect test

[0105] The fatigue resistance of mice was tested using a weight-bearing swimming test. Sixty mice were randomly divided into six groups: a blank control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 10 mice in each group. Each group was administered the corresponding sample by gavage at a dose of 500 mg / kg / day for 30 consecutive days. One hour after the last administration, a weight-bearing swimming test (with lead weights at the base of the tail bearing 5% of the body weight) was conducted, and the time to exhaustion was recorded.

[0106] Table 1. Results of memory ability test:

[0107]

[0108] As can be seen from the data in Table 1, the Example 1 and Example 2 groups are significantly better than the comparative groups in improving memory ability, which proves the importance of the synergistic effect of each component in the composition of the present invention.

[0109] Table 2 Results of immune regulation function test:

[0110]

[0111] As can be seen from the data in Table 2, the Example 1 group and the Example 2 group were significantly better than the comparative example and the model control group in terms of immune regulation function, which proves the importance of the synergistic effect of each component in the composition of the present invention.

[0112] Table 3. Results of anti-fatigue effect test:

[0113]

[0114] As can be seen from the data in Table 3, the Example 1 group and the Example 2 group were significantly better than the control groups in prolonging the time of mice swimming to exhaustion.

Claims

1. A composition containing ginseng extract, characterized in that, It is made from the following substances in parts by weight: 5-30 parts of ginseng peptide composition with a molecular weight of less than 3000 Da, 1-15 parts of saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2, 3-10 parts of ginseng polysaccharide-selenium nanocrystal composition, 1-5 parts of Lactobacillus plantarum P-8 lyophilized powder, 0.5-3 parts of sodium tanshinone, 3-20 parts of prebiotic composition, and 0.1-5 parts of vitamin composition; The preparation method of the ginseng peptide composition is as follows: S11. Raw material pretreatment: Take five-year-old fresh ginseng, wash it, and then use liquid nitrogen quick-freezing technology to treat it at -196℃ for 30 minutes, followed by rapid thawing. This freeze-thaw cycle can destroy cell structure and improve enzymatic hydrolysis efficiency. S12. Compound enzymatic hydrolysis: Adjust the pH of the pretreated ginseng slurry to 7.5, add V8 protease at 2% of the substrate mass, and enzymatically hydrolyze at 45℃ and normal pressure for 2 hours; S13. High hydrostatic pressure-assisted enzymatic hydrolysis: Adjust the pH to 7.0, add aminopeptidase at 1% of the substrate mass, place the mixture in a high hydrostatic pressure reactor, and use staged pressure control. Apply 100 MPa for the first 2 hours and increase it to 200 MPa for the next 2 hours. Maintain the temperature at 50℃. S14. Peptide separation and purification: After inactivating the enzyme in the solution after the enzymatic hydrolysis in step S13, centrifuge to collect the supernatant. After passing it through an ultrafiltration membrane with a molecular weight cutoff of 3000 Da, spray dry to obtain a ginseng peptide composition with a molecular weight of less than 3000 Da, and package it in nitrogen for later use. The preparation method of the saponin composition composed of rare ginsenosides Rg3, Rg5, and Rh2 is as follows: S21. Raw material extraction: Take dried ginseng root, crush it, add 70% ethanol, and reflux at 80℃ for 3 times, 2 hours each time. Combine the extracts. S22. Preliminary purification: The extract is concentrated under reduced pressure until there is no alcohol odor, and then passed through a macroporous adsorption resin column. First, impurities are removed by elution with water, and then 50% ethanol is used for elution. The eluent is collected. S23. Probiotic conversion: Concentrate the above eluent to a ginsenoside concentration of 100 mg / mL, and add Lactobacillus plantarum P-8 bacterial suspension at a volume ratio of 10:1, with a bacterial count ≥ 1 × 10⁻⁶. 9 CFU / mL, fermented under anaerobic conditions at 37℃ for 60 hours; S24. Purification of conversion products: The supernatant was collected by centrifugation of the fermentation broth and separated by high-performance pre-liquid chromatography to collect the Rg3, Rg5 and Rh2 fractions. The preparation method of the ginseng polysaccharide-selenium nanocrystal composition is as follows: S31. Ginseng polysaccharide extraction: Take the residue after ginseng extraction, add 90℃ hot water to extract 3 times, 2 hours each time, combine the extracts, concentrate and precipitate with alcohol, collect the precipitate to obtain ginseng polysaccharide; S32. Preparation of nanocrystals: Ginseng polysaccharide and sodium selenite were dissolved in deionized water at a mass ratio of 8:1 to prepare a 5% concentration solution. Vitamin C at 0.2% of the mass of ginseng polysaccharide was added as a reducing agent. The mixture was reacted in a water bath at 70°C for 3 hours. After filtration and drying, a ginseng polysaccharide-selenium nanocrystal composition was obtained. The prebiotic composition is a mixture of xylooligosaccharides, galactooligosaccharides, and resistant dextrin in a mass ratio of 1:1:2; The vitamin composition is a mixture of vitamin C, vitamin D and vitamin E in a mass ratio of 10:0.005:

2.

2. A method for preparing the composition containing ginseng extract as described in claim 1, characterized in that, The preparation method involves mixing a rare ginsenoside and ginseng peptide composition with a ginseng polysaccharide-selenium nanocrystal composition solution in a certain proportion, loading the mixture under gentle stirring for 24 hours to form a carrier system, then adding other components in a certain proportion and mixing evenly, preparing granules using a wet granulation process, adding magnesium stearate lubricant after drying, and compressing into tablets or filling into enteric-coated capsules. Enteric-coated capsules can ensure that the composition is released in a specific part of the intestine, maximizing the survival rate of probiotics and the conversion efficiency of saponins.

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