Growth regulation composition for increasing peanut yield and improving quality

Abstract

The invention relates to the technical field of plant growth regulation, and discloses a growth regulation composition for increasing peanut yield and improving peanut quality, the dosage form of the composition is a soluble concentrate, the effective components of the composition are aminobutyric acid and choline chloride, the weight ratio of the aminobutyric acid to the choline chloride is 5: 6, the composition is scientific and reasonable in matching, and the composition is suitable for large-scale production. The pesticide composition can obviously regulate the growth of peanuts, increase the pod number of plants, improve the hundred-kernel weight of peanuts, has obvious yield-increasing effect, can also improve the quality of peanuts and increase the protein content, fat content, oleic acid content and linoleic acid content of peanuts, has comprehensive effect, gives consideration to both pod increase and hundred-kernel weight increase, stable yield increase and quality improvement compared with a control agent, is safe to peanuts, and has wide application prospects. And the economic benefit is effectively improved.

Description

Technical Field

[0001] This invention belongs to the field of biological growth regulation technology, specifically relating to a plant growth regulator composition for improving peanut yield and quality. The active ingredients of the composition are aminobutyric acid and choline chloride. Background Technology

[0002] Gamma-aminobutyric acid (GABA) has a dual role as a metabolite and a signaling molecule in plants. It promotes photosynthesis and energy metabolism, promotes vegetative and reproductive growth, enhances the plant's resistance to various adversities, promotes the absorption of micronutrients, and regulates the levels of plant signaling molecules (endogenous hormones). It can stimulate crop growth and enhance stress resistance, and significantly promotes the absorption of micronutrients by crops.

[0003] Besides being used as a feed additive, choline chloride is also a broad-spectrum plant growth regulator. It can be absorbed through the stems, leaves, and roots of plants and then quickly translocated to the sites of action. Its physiological effects include inhibiting light absorption in C3 plants, promoting root development, and maximizing the accumulation of photosynthetic products in tubers and rhizomes, thereby increasing yield and improving quality.

[0004] During peanut growth, especially in the pegging stage, the root system develops rapidly, and the stems and leaves grow vigorously, resulting in a rapid increase in leaf area. This excessive vegetative growth leads to insufficient nutrition for the pods, resulting in small and few pods and underdeveloped kernels, thus affecting yield and quality. The applicant's experiments have shown that mixing aminobutyric acid (GABA) with choline chloride, which has a different mechanism of action, to control peanut growth and development can effectively control excessive stem and leaf growth, thereby increasing peanut yield and improving peanut quality. Summary of the Invention

[0005] The purpose of this invention is to provide a plant growth regulator composition, which is formulated into a soluble agent that can effectively inhibit the vegetative growth of the above-ground parts, dwarf the plants, darken the leaf color, and improve the transport and distribution of photosynthetic products, thus having a significant synergistic effect on increasing peanut yield and improving quality.

[0006] The technical solution of this invention is:

[0007] A growth regulator composition for increasing peanut yield and improving quality is characterized in that: the effective active ingredients of the composition are aminobutyric acid and choline chloride, the formulation of the composition is a soluble concentrate, wherein the weight ratio of aminobutyric acid to choline chloride is 5:6.

[0008] Further, the composition, based on 1000 parts by weight, contains the following components: 25-250 parts of GABA, 30-300 parts of choline chloride, 30-450 parts of solvent, 10-250 parts of emulsifier, 10-80 parts of antifreeze, 0-30 parts of defoamer, 0-40 parts of preservative, 0-20 parts of pH adjuster, and water as the balance.

[0009] Further, the composition, based on 1000 parts by weight, contains the following components: 50-200 parts of GABA, 60-240 parts of choline chloride, 100-300 parts of solvent, 50-200 parts of emulsifier, 20-60 parts of antifreeze, 0-20 parts of defoamer, 0-20 parts of preservative, 5-15 parts of pH adjuster, and the balance being water.

[0010] Further, the composition, based on 1000 parts by weight, contains the following components: 100 parts of GABA, 120 parts of choline chloride, 100-200 parts of solvent, 100-160 parts of emulsifier, 30-50 parts of antifreeze, 0-10 parts of defoamer, 0-10 parts of preservative, 8-12 parts of pH adjuster, and the balance being water.

[0011] Furthermore, the total weight of GABA and choline chloride in the composition is 55-550 g / L, optionally 110-440 g / L, and optionally 220 g / L.

[0012] Further, the solvent is selected from one or more of cyclohexanone, 200# solvent oil, 150# solvent oil, vegetable oil, xylene, tetrahydrofurfuryl alcohol, and NN dimethylacetamide; the emulsifier is selected from one or more of sodium dodecyl sulfate, calcium dodecylbenzene sulfonate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether, polyol fatty acid esters and their ethylene oxide adducts, and polyoxyethylene polyoxypropylene block copolymers; the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, and sorbitol; the defoamer is selected from one or more of silicone defoamers, C10-20 saturated fatty acid compounds, C8-10 fatty alcohols, hexanol, butanol, and octanol; the preservative is selected from one or more of sodium benzoate, calcium propionate, and potassium sorbate; and the pH adjuster may be selected from one or more of glacial acetic acid, citric acid, triethanolamine, light calcium carbonate, and potassium dihydrogen phosphate.

[0013] Furthermore, the composition is used to increase peanut yield and improve peanut quality.

[0014] Furthermore, the composition is used to increase the protein content, fat content, oleic acid content, and linoleic acid content of peanuts.

[0015] Furthermore, the dosage of the composition used to regulate peanut growth is 180–500 g / ha, with optional dosages of 198–462 g / ha and optional dosages of 231–264 g / ha.

[0016] The advantages of this invention are: (1) Compared with single agents, it has a good synergistic effect on increasing peanut yield and improving quality; (2) It can effectively increase peanut yield, increase the content of peanut protein, fat, oleic acid and linoleic acid, increase the economic value of peanut and increase farmers' income; (3) It is fast-acting and long-lasting, and the effect is not affected by rain 8 hours after application. It is convenient to apply during the rainy season; (4) It is easy to use, safe, low toxicity to humans and animals, does not contain residues, and is safe for peanuts. Detailed Implementation

[0017] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0018] Application Example 1

[0019] Example 1: 220 g / L choline chloride·aminobutyric acid soluble concentrate

[0020] Based on 1000 parts by weight, it contains 100 parts of GABA, 120 parts of choline chloride, 50 parts of cyclohexanone, 50 parts of 150# solvent oil, 20 parts of polyol fatty acid esters and their ethylene oxide adducts, 100 parts of calcium dodecylbenzenesulfonate, 40 parts of ethylene glycol, 5 parts of sodium benzoate, 2 parts of organosilicone defoamer, 1 part of citric acid, and water to 1000 parts.

[0021] Example 2: 220 g / L choline chloride·aminobutyric acid soluble concentrate

[0022] Based on 1000 parts by weight, it contains 100 parts of aminobutyric acid, 120 parts of choline chloride, 50 parts of 200# solvent oil, 50 parts of xylene, 40 parts of fatty alcohol polyoxyethylene ether, 80 parts of calcium dodecylbenzenesulfonate, 50 parts of propylene glycol, 5 parts of potassium sorbate, 2 parts of C8-10 fatty alcohol, 2 parts of potassium dihydrogen phosphate, and water to 1000 parts.

[0023] Example 3: 220 g / L choline chloride·aminobutyric acid soluble concentrate

[0024] Based on 1000 parts by weight, it contains 100 parts of GABA, 120 parts of choline chloride, 50 parts of 150# solvent oil, 50 parts of NN dimethylacetamide, 30 parts of polyoxyethylene polyoxypropylene block copolymer, 100 parts of sodium dodecyl sulfate, 40 parts of glycerol, 3 parts of sodium benzoate, 3 parts of C10-20 saturated fatty acid compounds, 1 part of citric acid, and water to 1000 parts.

[0025] Example 4: 110 g / L choline chloride·aminobutyric acid soluble concentrate

[0026] Based on 1000 parts by weight, it contains 50 parts of aminobutyric acid, 60 parts of choline chloride, 30 parts of xylene, 70 parts of vegetable oil, 50 parts of fatty alcohol polyoxyethylene ether, 60 parts of alkylphenol polyoxyethylene ether, 50 parts of ethylene glycol, 3 parts of potassium sorbate, 3 parts of organosilicone defoamer, 1 part of potassium dihydrogen phosphate, and water to 1000 parts.

[0027] Example 5: 330 g / L choline chloride·aminobutyric acid soluble concentrate

[0028] Based on 1000 parts by weight, it contains 150 parts of GABA, 180 parts of choline chloride, 70 parts of N,N dimethylacetamide, 40 parts of tetrahydrofurfuryl alcohol, 70 parts of fatty alcohol polyoxyethylene ether phosphate, 90 parts of polyol fatty acid esters and their ethylene oxide adducts, 50 parts of urea, 4 parts of sodium benzoate, 2 parts of organosilicone defoamer, 1 part of glacial acetic acid, and water to 1000 parts.

[0029] Example 6: 440 g / L choline chloride·aminobutyric acid soluble concentrate

[0030] Based on 1000 parts by weight, it contains 200 parts of aminobutyric acid, 240 parts of choline chloride, 90 parts of N,N-dimethylacetamide, 50 parts of xylene, 60 parts of polyoxyethylene-polyoxypropylene block copolymer, 100 parts of fatty alcohol polyoxyethylene ether phosphate, 40 parts of sorbitol, 6 parts of potassium sorbate, 3 parts of C8-10 fatty alcohol defoamer, 2 parts of glacial acetic acid, and water to 1000 parts.

[0031] Control single agent 1: 10% GABA soluble concentrate, commercially available.

[0032] Control single-dose 2: 60% choline chloride aqueous solution, commercially available.

[0033] Implementation Example 2:

[0034] Report on the Indoor Combined Toxicity Assay of Choline Chloride and GABA in Regulating Peanut Growth

[0035] Test target: peanut plant growth.

[0036] Experimental method: foliar spraying

[0037] Test basis: Refer to NY / T 2061.2-2011 and NY / T 2061.5-2016.

[0038] 1. Experimental Objective

[0039] Using GABA and choline chloride as test agents, and peanuts at the initial budding stage as the test subject, the optimal ratio of the combined use was screened in the laboratory using the foliar spray method to determine the optimal ratio of the two agents and to determine the formula. This provides a basis for formula screening for the rational mixing of the two agents and the development of effective agents for regulating peanut growth. The test results are reported as follows.

[0040] 2. Cultivation conditions

[0041] Peanuts were cultivated in pots until they began to bud.

[0042] 3 Experimental Design

[0043] 3.1 Reagents

[0044] 99% GABA technical grade, provided by the applicant;

[0045] The 98% choline chloride technical grade was provided by the applicant.

[0046] 3.2 Test Treatment

[0047] 3.2.1 Dosage setting

[0048] Based on the individual activity of the two agents and the characteristics of indoor testing, and on the basis of the preliminary test, the dosage of GABA and choline chloride were set to different combinations, as shown in Table 1, for a total of 15 groups.

[0049] An aqueous solution containing the same solvent and emulsifier but without any reagent was used as a blank control for in-house bioassays.

[0050] Table 1 Experimental reagent dosing design

[0051]

[0052]

[0053] 3.2.2 Experimental Repetition

[0054] Each concentration of the experimental agent was divided into 4 replicates, with 20 pots per replicate and 1 peanut plant per pot.

[0055] 3.3 Processing Method

[0056] The experiment involved two applications of the pesticide. The first application was performed when the peanut plants began to bud, and the second application was performed when the peanuts began to peg.

[0057] 4. Data Survey and Statistical Analysis

[0058] 4.2 Data Statistical Analysis

[0059] The theoretical inhibition rate (growth rate) E0 (E0=X+YX*Y / 100) of each treatment combination was calculated using the Gowing method. Then, it was compared with the measured inhibition rate (growth rate) (E) to evaluate the combined effect of the two treatments on peanuts.

[0060] When the E-E0 value is greater than 10%, it indicates a synergistic effect; when the E-E0 value is less than -10%, it indicates an antagonistic effect; and when the E-E0 value is between -10% and 10%, it indicates an additive effect. The optimal ratio is determined based on factors such as the actual plant height, the characteristics of the two plant growth regulators, and the balance of the formulation.

[0061] In the formula, X is the growth rate of the number of pods per plant (the growth rate of yield per plant) when the amount of GABA is P; Y is the growth rate of the number of pods per plant (the growth rate of yield per plant) when the amount of choline chloride is Q.

[0062] 5 Results and Analysis

[0063] Table 2. Indoor combined effect of choline chloride and GABA and their different ratios on peanuts.

[0064]

[0065] As shown in Table 2, after the experimental agents were mixed, the E-E0 value of the growth rate of the number of pods per plant in the treatment of 200 mg / L GABA + 240 mg / L choline chloride (5:6) was 5.11, indicating an additive effect. The E-E0 value of the growth rate of yield per plant was 10.11, which is greater than 10%, indicating a synergistic effect. This treatment had a significant regulatory effect on the increase of the number of pods per plant and the increase of yield per plant, and had a certain synergistic effect.

[0066] Observations during the experiment showed that the two agents and their respective dosage ratios were safe for the growth of the experimental peanuts and did not cause any phytotoxicity.

[0067] Application Example 3: Field efficacy experiment of choline chloride-aminobutyric acid soluble concentrate in regulating peanut growth (Examples 1-6)

[0068] 1. Experimental Objective

[0069] To verify the regulatory effect of the choline chloride-aminobutyric acid soluble concentrate developed by the applicant on peanut growth, the appropriate timing of application, the recommended dosage, and its safety on peanut growth, so as to provide a basis for the registration of the product.

[0070] 2 Materials and Methods

[0071] Application method: spraying, water consumption 450-750 liters / hectare.

[0072] 2.1 Treatment of experimental reagents

[0073] Table 3 Treatment of test reagents

[0074]

[0075]

[0076] 2.2 Experimental subjects and crops

[0077] Experimental subject: peanut. Peanuts grew well after emergence and had a growth period of about 180 days.

[0078] 2.3 Application Method

[0079] Application time: Foliar spraying should be carried out during the initial budding and pegging stages, with an interval of about 10 to 15 days between the two sprayings. The spray should be applied at a constant volume, and the spray should be uniform and consistent, without overlapping or missing areas. A blank spray of the same amount of water should be applied.

[0080] 2.4 Survey Time, Frequency, and Methods

[0081] A survey was conducted before the first application of pesticide, 15 days after the second application, and at peanut harvest. After the peanut harvest, a quality survey was conducted to determine the content of protein, fat, oleic acid, linoleic acid, and other qualities. A total of four surveys were conducted.

[0082] 2.5 Method for Calculating Drug Efficacy

[0083] Increase rate (%) = [(treatment output - control output) / control output] × 100.

[0084] 3 Results and Analysis

[0085] Table 4. Results of the experiment on the effects of the test agents on peanut yield and quality (Part 1)

[0086]

[0087]

[0088] Table 5. Results of the experiment on the effects of the test agents on peanut yield and quality (Part II)

[0089]

[0090]

[0091] 4. Conclusion

[0092] Suitable application time and dosage: The experimental agents were applied once each during the initial budding stage and the pegging stage of peanuts. It can be seen that the two concentrations of the tested agents (Examples 1-6, choline chloride·aminobutyric acid soluble concentrate) promoted the number of peanut branches, number of pods per plant, number of kernels per plant, 100-kernel pod weight, and 100-kernel weight to a certain extent. After application, the agents reduced plant growth, increased peanut yield, and improved peanut quality, such as protein, fat, oleic acid, and linoleic acid content. The effects were significantly better than the control agent and the blank control. Analysis of variance showed that, compared with the control agent, the tested agents (Examples 1-6) significantly improved the number of peanut branches, number of pods per plant, number of kernels per plant, 100-kernel pod weight, and 100-kernel weight, significantly better than the blank control and significantly better than the control agent. They also showed an improving effect on peanut quality, significantly better than the blank control, and with no significant difference or slightly more significant difference than the control agent.

[0093] Application Example 4: Safety Evaluation Experiment of Peanuts Using Choline Chloride-GABA Soluble Solution (Examples 1-6)

[0094] 1. Experimental Objective

[0095] Verify the indoor safety of the choline chloride-aminobutyric acid soluble concentrate developed by the applicant for peanuts.

[0096] 2 Materials and Methods

[0097] Peanuts were used as the test target. Three common varieties were selected: "Huayu 22", "Huayu 20", and "Luhua 14". All seeds were purchased from the market. One seed was planted per pot and cultured indoors. Peanuts with uniform growth were selected as experimental materials when they reached the initial budding stage. Application method: spraying; water treatment served as a control.

[0098] 2.1 Treatment of experimental reagents

[0099] Table 6 Treatment of Test Reagents

[0100]

[0101] 2.2 Application Method

[0102] Application time: Foliar spraying should be carried out during the initial budding and pegging stages, with an interval of about 10 to 15 days between the two sprayings.

[0103] 2.3 Survey Time, Frequency, and Methods

[0104] Three days after each application, the damage to peanuts was investigated, and the presence or absence of pesticide damage symptoms was recorded, along with a description of the degree of damage. Sixty days after the second application, an absolute value survey method was used to investigate the main stem height, number of pods per plant, and yield per plant.

[0105] 3 Results and Analysis

[0106] Table 7. Effects of the test agents on peanut yield and quality. (Test results)

[0107]

[0108] 4. Conclusion

[0109] The experiment showed that when the tested pesticides were applied to different peanut varieties at effective ingredient dosages of 264, 528, and 1056 g / ha, and at the initial budding and pegging stages, for a total of two applications three days after each application, no necrosis or wilting symptoms of pesticide damage were observed. Compared with the control, the leaves of each treatment turned greener. Furthermore, the pesticides significantly increased the number of pods per plant and the yield per plant, with increases ranging from 12.34% to 23.24% in the number of pods per plant and from 15.03% to 22.15% in yield per plant, demonstrating a strong synergistic effect.

[0110] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. A growth regulator composition for increasing peanut yield and improving quality, characterized in that: The effective active ingredients of the composition are aminobutyric acid and choline chloride, and the dosage form of the composition is a soluble concentrate, wherein the weight ratio of aminobutyric acid to choline chloride is 5:

6. The composition, based on 1000 parts by weight, contains the following components: 25-250 parts of GABA, 30-300 parts of choline chloride, 30-450 parts of solvent, 10-250 parts of emulsifier, 10-80 parts of antifreeze, 0-30 parts of defoamer, 0-40 parts of preservative, 0-20 parts of pH adjuster, and water as the balance.

2. The composition according to claim 1, characterized in that: The total weight of GABA and choline chloride in the composition is 55-550 g / L, optionally 110-440 g / L, and optionally 220 g / L.

3. The composition according to claim 1 or 2, characterized in that: The composition, based on 1000 parts by weight, contains the following components: 50-200 parts of GABA, 60-240 parts of choline chloride, 100-300 parts of solvent, 50-200 parts of emulsifier, 20-60 parts of antifreeze, 0-20 parts of defoamer, 0-20 parts of preservative, 5-15 parts of pH adjuster, and water as the balance.

4. The composition according to claim 3, characterized in that: The composition, based on 1000 parts by weight, contains the following components: 100 parts of GABA, 120 parts of choline chloride, 100-200 parts of solvent, 100-160 parts of emulsifier, 30-50 parts of antifreeze, 0-10 parts of defoamer, 0-10 parts of preservative, 8-12 parts of pH adjuster, and the balance being water.

5. The composition according to any one of claims 1 to 4, characterized in that: The composition is used to increase peanut yield and improve peanut quality.

6. The composition according to claim 5, characterized in that: The composition is used to increase the protein content, fat content, oleic acid content and linoleic acid content of peanuts.

7. The composition according to claim 5 or 6, characterized in that: The composition is used to regulate the effective dosage of peanut growth at a rate of 180-500 g / ha, with optional application rates of 198-462 g / ha and optional application rates of 231-264 g / ha.

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