Purification device for cell supernatant exosome
By setting up observation devices and dust-proof devices in the cell supernatant exosome purification device, the problem of ultracentrifuge waiting for static start is solved, the work efficiency and equipment safety are improved, and the cleaning frequency and cost are reduced, ensuring experiments Accuracy of results and stable operation of equipment.
Patent Information
- Application Number
- CN202420249167.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-01
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-02-01
AI Technical Summary
When purifying and isolating cell supernatant exosomes, existing ultracentrifuges need to wait for the reagent tube to rest before starting the device, resulting in inefficiency and may cause sample mixing or spillage, affecting experimental results and damaging the device.
A cell supernatant exosome purification device including observation device is designed, using circular grooves, sliders, lenses, limit rods and pull rings, allowing staff to easily observe whether the bucket is hanging stably and ensure the safe operation of the equipment. , and reduce dust adhesion through dustproof devices, improving equipment cleaning frequency and cost-effectiveness.
Improve work efficiency, ensure safe operation of equipment, reduce safety hazards and equipment failures caused by unstable buckets, and reduce cleaning frequency and cost through dustproof devices, maintaining the cleaning and display effect of the control panel.
Smart Images
Figure CN222343137U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biotechnology, and in particular to a device for purifying cell supernatant exosomes. Background Art
[0002] Exosomes in the cell supernatant refer to small vesicles secreted by cells that contain biological molecules such as proteins, nucleic acids, and lipids. They can transmit information between cells, regulate the internal and external environments of cells, and participate in various biological processes. For the research and application of exosomes in the cell supernatant, they need to be purified. Purification can help researchers remove impurities. Purification equipment usually uses an ultracentrifuge to separate solid particles from liquids in exosomes, thereby achieving the purpose of purification and separation.
[0003] The above-mentioned and existing related technologies often have the following defects: when using an ultracentrifuge to purify and separate exosomes, it is usually necessary to place the reagent tube on a horizontal rotor. After placement, the staff needs to wait for a few minutes for the reagent tube to be in a stationary state before starting the equipment. This waiting method is not only inconvenient for the staff to observe whether the reagent tube is in a stationary state, thereby reducing work efficiency. If the equipment is started before the reagent tube is in a stationary state, it is easy to cause the sample in the reagent tube to mix or overflow, affecting the experimental results or even damaging the equipment.
[0004] Therefore, the present invention provides a device for purifying exosomes in cell supernatant. Utility Model Content
[0005] The purpose of the utility model is to solve the problem in the prior art that the staff need to wait for several minutes for the reagent tube to be in a static state before starting the equipment. This waiting method is not only inconvenient for the staff to observe whether the reagent tube is in a static state, but also reduces work efficiency. A purification device for exosomes from cell supernatant is proposed.
[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a device for purifying exosomes in cell supernatant, comprising a body, the upper surface of the body being fixedly connected to a control panel, the inner wall of the body being slidably connected to a hatch, the inner wall of the body being installed with a horizontal rotor, the surface of the body being provided with an observation device, the observation device comprising a circular groove, the circular groove being opened on the inner wall of the body, the inner wall of the circular groove being slidably connected to a slider, the surface of the slider being fixedly connected to a fixing plate, the surface of the fixing plate being fixedly connected to a lens, the surface of the fixing plate slidingly passing through a limiting rod, one end of the limiting rod being fixedly connected to a pull ring, the other end of the limiting rod being rotatably connected to a ball, and the limiting rod being slidably connected to the inner wall of the body.
[0007] The effects achieved by the above components are: by setting up an observation device and utilizing the mutual cooperation among the circular groove, slider, fixing plate, lens, limit rod, round ball and pull ring, the staff can easily and conveniently observe whether the bucket is hung firmly, which helps to timely discover abnormal conditions of the bucket and allows the staff to take quick measures when problems are found to avoid safety hazards or equipment failures caused by unstable buckets. The convenient observation method not only improves work efficiency, but also ensures the safe operation of ultracentrifuge equipment.
[0008] Preferably, the arc surface of the limiting rod is sleeved with a spring, and both ends of the spring are fixedly connected to the limiting rod and the fixing plate respectively.
[0009] The effect achieved by the above components is: when the pull ring is loosened, the spring begins to press the limit rod against the inner wall of the body with the help of its own elastic force. The spring fixes the position of the limit rod to prevent the limit rod from being out of contact with the fixing plate.
[0010] Preferably, a plurality of balls are rotatably connected to the surface of the slider, and the plurality of balls are slidably connected to the inner wall of the circular groove.
[0011] The effect achieved by the above components is: the slider drives the ball to rotate, and the ball slides along the inner wall of the circular groove. The ball reduces the friction between the slider and the circular groove, thereby driving the fixed plate to rotate more conveniently and smoothly.
[0012] Preferably, the lens is made of acrylic plate.
[0013] The effect achieved by the above components is that the fixed plate will also drive the lens to rotate, so that the staff can observe whether the bucket on the horizontal rotor is hung firmly. The lens is made of acrylic plate. The lens made of acrylic plate is not easy to break, so it is safer during the use of ultracentrifuge. The lens made of acrylic plate is also more durable.
[0014] Preferably, the surface of the control panel is provided with a dustproof device, which includes two L-plates, the short arms of the two L-plates are fixedly connected to the control panel, the long arms of the two L-plates are rotatably connected to a fixed rod, the arc surface of the fixed rod is fixedly connected to a dustproof cloth, and the surface of the control panel is fixedly connected to a limiting column.
[0015] The above components achieve the following effects: By installing a dustproof device and utilizing the interaction between the L-plate, fixing rod, circular plate, dustproof cloth, and limiting posts, the control panel can be dustproofed. This effectively blocks dust, fine particles, and other impurities in the air, reducing their adhesion to the control panel surface, thereby protecting the screen from dust. The addition of the dustproof cloth can reduce dust accumulation on the control panel surface, reducing the frequency of cleaning, saving cleaning time and cost. Furthermore, keeping the control panel surface clean can improve display quality and prevent dust from affecting the visual experience.
[0016] Preferably, the arc surface of the fixing rod is fixedly connected to two circular plates, the arc surface of the fixing rod is sleeved with two torsion springs, and the two ends of the two torsion springs are respectively fixedly connected to the circular plate and the long arm of the L-plate.
[0017] The effect achieved by the above components is: when the dustproof cloth is loosened, the torsion spring begins to automatically retract the dustproof cloth with its own elastic force, and the torsion spring achieves the effect of automatically retracting and resetting the dustproof cloth, thereby improving work efficiency.
[0018] Preferably, a limiting groove is provided on the surface of the limiting column, and the limiting groove is adapted to the thickness of the dustproof cloth.
[0019] The effect achieved by the above components is: the holes opened in the dustproof cloth are inserted into the limiting grooves opened on the surface of the limiting column. The limiting grooves can limit the position of the dustproof cloth to prevent the dustproof cloth from losing contact with the limiting column due to the surface of the limiting column being too smooth.
[0020] In summary:
[0021] 1. In the present invention, by providing an observation device and utilizing the mutual cooperation among the circular groove, slider, fixing plate, lens, limit rod, round ball and pull ring, the staff can easily and conveniently observe whether the bucket is hung steadily, which helps to timely discover abnormal conditions of the bucket and enables the staff to take quick measures when problems are found to avoid safety hazards or equipment failures caused by unstable buckets. The convenient observation method not only improves work efficiency, but also ensures the safe operation of the ultracentrifuge equipment.
[0022] 2. In this utility model, by providing a dustproof device and utilizing the interaction between the L-plate, fixing rod, circular plate, dustproof cloth, and limiting posts, the control panel can be dustproofed. This effectively blocks dust, fine particles, and other impurities in the air, reducing their adhesion to the control panel surface, thereby protecting the screen from dust. The addition of the dustproof cloth can reduce dust accumulation on the control panel surface, reducing the frequency of cleaning, saving cleaning time and cost. Furthermore, keeping the control panel surface clean can improve the display quality and prevent dust from affecting the visual experience. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;
[0024] Figure 2 This is a structural diagram of the utility model from another perspective;
[0025] Figure 3 This is a schematic structural diagram of the horizontal rotor of the utility model;
[0026] Figure 4 For this utility model Figure 3 A magnified view of point A;
[0027] Figure 5 This is a schematic diagram of the structure of the observation device of the utility model;
[0028] Figure 6 This is a schematic structural diagram of the dust-proof device of the present utility model.
[0029] Legend: 1. Body; 2. Control panel; 3. Horizontal rotor; 4. Observation device; 41. Circular groove; 42. Slider; 43. Fixing plate; 44. Lens; 45. Limit rod; 46. Round ball; 47. Pull ring; 48. Ball bearing; 49. Spring; 5. Dustproof device; 51. L-plate; 52. Fixing rod; 53. Circular plate; 54. Dustproof cloth; 55. Torsion spring; 56. Limit column; 57. Limiting groove; 6. Hatch door. DETAILED DESCRIPTION
[0030] Reference Figure 1 and Figure 2 As shown, the utility model provides a technical solution: a purification device for cell supernatant exosomes, comprising a body 1, a control panel 2 fixedly connected to the upper surface of the body 1, a hatch 6 slidably connected to the inner wall of the body 1, a horizontal rotor 3 installed on the inner wall of the body 1, an observation device 4 provided on the surface of the body 1, and a dustproof device 5 provided on the surface of the control panel 2.
[0031] The specific settings and functions of the observation device 4 and the dust-proof device 5 are described in detail below.
[0032] Reference Figure 3 and Figure 4 as well as Figure 54, and the like. As shown in FIG. 4 , the observation device 4 includes a circular groove 41, which is provided on the inner wall of the machine body 1. A slider 42 is slidably connected to the inner wall of the circular groove 41. A fixing plate 43 is fixedly connected to the surface of the slider 42. A lens 44 is fixedly connected to the surface of the fixing plate 43. The surface of the fixing plate 43 slides through a limit rod 45. One end of the limit rod 45 is fixedly connected to a pull ring 47. The other end of the limit rod 45 is rotatably connected to a ball 46. The limit rod 45 is slidably connected to the inner wall of the machine body 1. By setting the observation device 4 and utilizing the mutual cooperation between the circular groove 41, the slider 42, the fixing plate 43, the lens 44, the limit rod 45, the ball 46 and the pull ring 47, the staff can easily and conveniently observe whether the bucket is hung firmly, which helps to timely discover the abnormal situation of the bucket and allows the staff to take quick measures when problems are found to avoid safety hazards or equipment failures caused by the instability of the bucket. The convenient observation method not only improves work efficiency, but also ensures the safe operation of the ultracentrifuge equipment. 9. The two ends of the spring 49 are fixedly connected to the limit rod 45 and the fixed plate 43 respectively. Loosen the pull ring 47. The spring 49 begins to use its own elastic force to limit the rod 45 against the inner wall of the body 1. The spring 49 fixes the position of the limit rod 45 to prevent the limit rod 45 from being out of contact with the fixed plate 43. The surface of the slider 42 is rotatably connected with a plurality of balls 48. The plurality of balls 48 are all slidably connected to the inner wall of the circular groove 41. The slider 42 will drive the balls 48 to rotate. The balls 48 move along the circular groove. 41 slides on the inner wall, and the ball 48 reduces the friction between the slider 42 and the circular groove 41, thereby driving the fixed plate 43 to rotate more conveniently and smoothly. The lens 44 is made of acrylic plate, and the fixed plate 43 also drives the lens 44 to rotate, so that it is convenient for the staff to observe whether the bucket on the horizontal rotor 3 is hung firmly. The lens 44 is made of acrylic plate. The lens 44 made of acrylic plate is not easy to break, so it is safer during the use of the ultracentrifuge, and the lens 44 made of acrylic plate is also more durable.
[0033] Reference Figure 6As shown, specifically, the dustproof device 5 includes two L-plates 51, the short arms of the two L-plates 51 are fixedly connected to the control panel 2, and the long arms of the two L-plates 51 are rotatably connected to the fixing rod 52, the arc surface of the fixing rod 52 is fixedly connected to the dustproof cloth 54, and the surface of the control panel 2 is fixedly connected to the limiting column 56. By setting up the dustproof device 5, the mutual cooperation between the L-plate 51, the fixing rod 52, the circular plate 53, the dustproof cloth 54 and the limiting column 56 can be used to prevent dust from entering the control panel 2, effectively block impurities such as dust and fine particles in the air, and reduce the adhesion of these impurities to the surface of the control panel 2, thereby protecting the screen from the influence of dust. Adding the dustproof cloth 54 can reduce the dust deposition on the surface of the control panel 2, reduce the frequency of cleaning, and save cleaning time and cost. At the same time, keeping the surface of the control panel 2 clean can improve the display effect and prevent dust from affecting the visual experience. The arc surface of the fixing rod 52 is fixedly connected to two circular plates 53, and the arc surface of the fixing rod 52 is covered with two torsion springs 55. The two ends of the two torsion springs 55 are fixedly connected to the circular plate 53 and the long arm of the L plate 51 respectively. When the dustproof cloth 54 is loosened, the torsion spring 55 begins to automatically reel the dustproof cloth 54 with its own elastic force. The torsion spring 55 achieves the effect of automatically reeling and resetting the dustproof cloth 54, thereby improving work efficiency. A limiting groove 57 is provided on the surface of the limiting column 56, and the limiting groove 57 is adapted to the thickness of the dustproof cloth 54. The hole provided on the dustproof cloth 54 is put into the limiting groove 57 provided on the surface of the limiting column 56. The limiting groove 57 can limit the position of the dustproof cloth 54 to prevent the dustproof cloth 54 from being out of contact with the limiting column 56 due to the surface of the limiting column 56 being too smooth.
[0034] Working principle: In the process of purifying exosomes, an ultracentrifuge is needed to separate the components in the mixture through rotating centrifugal force. First, remove the horizontal rotor 3, then put the reagent tube into the bucket, then hang the bucket on the horizontal rotor 3, and then put the horizontal rotor 3 into the body 1. When putting the horizontal rotor 3, you need to use the observation device 4 to check whether the bucket is hung firmly. First pull the pull ring 47, the pull ring 47 will drive the limit rod 45 to slide along the surface of the fixed plate 43, so that the limit rod 45 is out of contact with the body 1. At this time, the limit rod 45 will pull The spring 49 is stretched, and then the fixing plate 43 is rotated. The fixing plate 43 drives the slider 42 to slide along the inner wall of the circular groove 41. At this time, the staff can loosen the pull ring 47. At this time, the spring 49 begins to press the limit rod 45 against the inner wall of the body 1 with its own elastic force. The spring 49 fixes the position of the limit rod 45 to prevent the limit rod 45 from being out of contact with the fixing plate 43. When the fixing plate 43 is rotated, the ball 46 is driven to rotate. The ball 46 reduces the friction between the limit rod 45 and the body 1. At the same time, the slider 42 will The movable ball 48 rotates and slides along the inner wall of the circular groove 41. The ball 48 reduces the friction between the slider 42 and the circular groove 41, thereby driving the fixed plate 43 to rotate more conveniently and smoothly. The fixed plate 43 also drives the lens 44 to rotate, so that it is convenient for the staff to observe whether the bucket on the horizontal rotor 3 is hung firmly. The lens 44 is made of acrylic plate. The lens 44 made of acrylic plate is not easy to break, so it is safer in the process of using the ultracentrifuge. The lens 44 made of acrylic plate is also more durable. After the observation is completed, the staff Close the hatch 6, adjust and start through the control panel 2, and by setting up the observation device 4, using the mutual cooperation between the circular groove 41, the slider 42, the fixing plate 43, the lens 44, the limit rod 45, the round ball 46 and the pull ring 47, the staff can easily and conveniently observe whether the bucket is hung firmly, which helps to timely discover abnormal conditions of the bucket and allows the staff to take quick measures when problems are found to avoid safety hazards or equipment failures caused by the instability of the bucket. The convenient observation method not only improves work efficiency, but also ensures the safe operation of the ultracentrifuge equipment.
[0035] After using the control panel 2, it is necessary to use the dustproof device 5 for dust prevention. First, pull the dustproof cloth 54, and the dustproof cloth 54 will drive the fixing rod 52 and the circular plate 53 to rotate along the long arm surface of the L plate 51. When the circular plate 53 rotates, it will squeeze the torsion spring 55. There are holes on the surface of the dustproof cloth 54, and then the holes are put into the limiting grooves 57 on the surface of the limiting column 56. The limiting grooves 57 can limit the position of the dustproof cloth 54 to avoid the situation where the dustproof cloth 54 is out of contact with the limiting column 56 due to the surface of the limiting column 56 being too smooth. When it is necessary to use the control panel 2, pull the dustproof cloth 54 to disengage the holes on the surface of the dustproof cloth 54 from contact with the limiting column 56, and then When the worker loosens the dustproof cloth 54, the torsion spring 55 begins to automatically retract the dustproof cloth 54 by virtue of its own elastic force. The torsion spring 55 achieves the function of automatically retracting and resetting the dustproof cloth 54, thereby improving work efficiency. By providing the dustproof device 5 and utilizing the interaction between the L-plate 51, the fixing rod 52, the circular plate 53, the dustproof cloth 54, and the limiting column 56, the control panel 2 can be dustproofed, effectively blocking dust, fine particles, and other impurities in the air, reducing the adhesion of these impurities to the surface of the control panel 2, thereby protecting the screen from dust. The addition of the dustproof cloth 54 can reduce dust deposition on the surface of the control panel 2, reduce the frequency of cleaning, and save cleaning time and cost. At the same time, keeping the surface of the control panel 2 clean can improve the display effect and prevent dust from affecting the visual experience.
[0036] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.
Claims
1. A purification device for exosomes in cell supernatant, comprising a body (1), a control panel (2) fixedly connected to the upper surface of the body (1), a door (6) slidably connected to the inner wall of the body (1), a horizontal rotor (3) installed on the inner wall of the body (1), and an observation device (4) provided on the surface of the body (1), characterized in that: The observation device (4) comprises a circular groove (41), wherein the circular groove (41) is provided on the inner wall of the body (1), a slider (42) is slidably connected to the inner wall of the circular groove (41), a fixing plate (43) is fixedly connected to the surface of the slider (42), a lens (44) is fixedly connected to the surface of the fixing plate (43), a limiting rod (45) is slidably penetrated through the surface of the fixing plate (43), one end of the limiting rod (45) is fixedly connected to a pull ring (47), the other end of the limiting rod (45) is rotatably connected to a round ball (46), and the limiting rod (45) is slidably connected to the inner wall of the body (1).
2. The device for purifying exosomes from cell supernatant according to claim 1, characterized in that: The arc surface of the limiting rod (45) is sleeved with a spring (49), and the two ends of the spring (49) are respectively fixedly connected to the limiting rod (45) and the fixing plate (43).
3. The device for purifying exosomes from cell supernatant according to claim 1, characterized in that: A plurality of balls (48) are rotatably connected to the surface of the slider (42), and the plurality of balls (48) are slidably connected to the inner wall of the circular groove (41).
4. The device for purifying exosomes from cell supernatant according to claim 1, characterized in that: The lens (44) is made of acrylic plate.
5. The device for purifying exosomes from cell supernatant according to claim 1, characterized in that: The surface of the control panel (2) is provided with a dustproof device (5), the dustproof device (5) comprising two L-plates (51), the short arms of the two L-plates (51) are fixedly connected to the control panel (2), the long arms of the two L-plates (51) are rotatably connected to a fixing rod (52), the circular arc surface of the fixing rod (52) is fixedly connected to a dustproof cloth (54), and the surface of the control panel (2) is fixedly connected to a limiting column (56).
6. The device for purifying exosomes from cell supernatant according to claim 5, characterized in that: The arc surface of the fixing rod (52) is fixedly connected to two circular plates (53), and the arc surface of the fixing rod (52) is sleeved with two torsion springs (55), and the two ends of the two torsion springs (55) are respectively fixedly connected to the circular plates (53) and the long arms of the L-plate (51).
7. The device for purifying exosomes from cell supernatant according to claim 5, characterized in that: A limiting groove (57) is provided on the surface of the limiting column (56), and the limiting groove (57) is adapted to the thickness of the dustproof cloth (54).