Tissue stem cell separation centrifugal machine

Abstract

The utility model relates to the technical field of centrifugal machines, in particular to a tissue stem cell separation centrifugal machine. According to the technical scheme, the device comprises a body, a centrifugal base is fixedly installed in the body, a containing box is fixedly installed on the centrifugal base, the device further comprises a containing plate, the containing plate is slidably installed in the containing box, a plurality of containing grooves arranged at equal intervals are formed in the containing plate, and a supporting plate is fixedly installed at the bottom of the containing box; a magnifying lens is slidably mounted on one side of the supporting plate and slidably connected with the containing box, and an auxiliary mechanism for driving the containing plate to vibrate is arranged on the containing box. According to the utility model, air bubbles between cells before centrifugation are removed, the precipitation condition of the cells after centrifugation is convenient to observe, the centrifugation effect is improved, and the operation is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of centrifuges, in particular to a tissue stem cell separation centrifuge. Background Art

[0002] The tissue stem cell separation centrifuge is a device used to separate and extract stem cells from tissues. Stem cells are a type of cell with self-renewal and multipotent differentiation capabilities, and have broad application prospects, such as regenerative medicine, tissue engineering, and drug screening.

[0003] Before centrifuging the cells, it is necessary to collect and process the sample containing the target tissue to obtain a cell suspension, add the separation medium and stir evenly to obtain the sample, and put the sample into a test tube for centrifugation. Since the stirring process introduces air into the liquid, air bubbles are generated in the sample, and the air bubbles will mix with the cell suspension. Since the cell mass is very low, the air bubbles will support the cells and prevent them from settling, resulting in uneven cell sedimentation or incomplete separation. Therefore, the present application proposes a tissue stem cell separation centrifuge. Summary of the invention

[0004] The utility model aims to provide a tissue stem cell separation centrifuge to solve the problem that air bubbles between cells cannot be removed in the background technology.

[0005] The technical solution of the utility model is: a tissue stem cell separation centrifuge, comprising a main body, a centrifugal base is fixedly installed in the main body, a placement box is fixedly installed on the centrifugal base, and further comprising:

[0006] A placement plate, the placement plate is slidably mounted inside the placement box, a plurality of placement slots arranged at equal distances are provided on the placement plate, a support plate is fixedly mounted on the bottom of the placement box, a magnifying glass is slidably mounted on one side of the support plate, the magnifying glass is slidably connected to the placement box, and an auxiliary mechanism for driving the placement plate to vibrate is provided on the placement box;

[0007] The driving mechanism is arranged in the placement box and is used to drive the placement plate to move.

[0008] Optionally, the auxiliary mechanism includes an extrusion block fixedly mounted on one side of the placement plate, a connecting column fixedly mounted on one side of the placement plate, the connecting column is slidably connected to the extrusion block, a spring is sleeved on the connecting column, one end of the spring is fixedly connected to the bottom of the extrusion block, and a connecting plate is fixedly mounted on the other side of the placement plate, the connecting plate is slidably connected to the placement box.

[0009] Optionally, the driving mechanism includes a connecting block fixedly mounted on the bottom of the support plate, the connecting block is arranged in a cross-section, a fixing frame is fixedly mounted on one side of the placement box, a threaded rod is threadedly mounted on the fixing frame, an extrusion column is rotatably mounted on one end of the threaded rod, the extrusion column is slidably connected to the placement box, and a rotating block is fixedly mounted on one end of the threaded rod.

[0010] Optionally, a protrusion is fixedly mounted on one side of the support plate, a telescopic rod is fixedly mounted on the protrusion, the telescopic rod is fixedly connected to the magnifying glass, a guide groove is formed on the inner wall of the placement box, and the protrusion is located in the guide groove.

[0011] Optionally, a limiting block is fixedly installed on the bottom of the connecting plate, a limiting rod is slidably installed on one side of the placement box, and one end of the limiting rod is located in the limiting block.

[0012] Optionally, a cylinder is fixedly mounted on the main body, a piston rod of the cylinder passes through one end of the main body and a slide is fixedly mounted thereon, the slide is slidably connected to the main body, and a heat preservation cover is fixedly mounted on the bottom of the slide.

[0013] Optionally, a slide groove is provided on the placement box, and the magnifying glass is located in the slide groove.

[0014] Compared with the prior art, the utility model has the following beneficial technical effects:

[0015] 1. By placing the test tube containing the cells to be centrifuged into the placement tank and pressing the connecting plate, the connecting plate drives the placement plate to move downward, and the extrusion block on one side of the placement plate squeezes the spring, and the spring compresses and deforms to rebound the placement plate, driving the test tube in the placement plate to vibrate, thereby removing the air bubbles in the test tube and improving the centrifugal effect;

[0016] 2. By rotating the rotating block, the rotating block drives the threaded rod to rotate. Since the threaded rod is threadedly connected to the fixed frame, as the threaded rod rotates, the extrusion column at one end of the threaded rod moves inward, extruding the bottom surface of the connecting block, driving the placement plate to move upward, so that the magnifying glass on one side of the support plate is moved out of the placement box together, realizing convenient observation of the cell state after centrifugation operation and improving convenience.

[0017] This practical new model can remove the air bubbles between cells before centrifugation, making it easier to observe the centrifugation process.

[0018] After the end, the cell sedimentation is improved, the centrifugation effect is improved, and the operation is convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A structural schematic diagram of an embodiment of the utility model is given;

[0020] Figure 2A schematic diagram of the structure of a placement box, a placement plate and a magnifying glass according to an embodiment of the utility model is given;

[0021] Figure 3 A schematic diagram of the structure of a placement box, a spring and a rotating block in one embodiment of the utility model is given;

[0022] Figure 4 A schematic diagram of the structure of a connecting plate and a limiting block in one embodiment of the utility model is given;

[0023] Figure 5 A schematic structural diagram of a threaded rod and an extruded column according to an embodiment of the utility model is given.

[0024] Figure numerals: 1. main body; 2. cylinder; 3. slide plate; 4. placement box; 5. insulation cover; 6. centrifugal base; 7. extrusion block; 8. support plate; 9. placement groove; 10. connecting plate; 11. magnifying glass; 12. telescopic rod; 13. bump; 14. placement plate; 15. spring; 16. connecting column; 17. limit rod; 18. limit block; 19. threaded rod; 20. fixing frame; 21. rotating block; 22. slide groove; 23. guide groove; 24. connecting block; 25. extrusion column. DETAILED DESCRIPTION

[0025] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and specific embodiments. Example

[0026] like Figure 1-2 As shown, a tissue stem cell separation centrifuge proposed by the utility model comprises a main body 1, a centrifugal base 6 is fixedly installed in the main body 1 for centrifugal rotation, a placement box 4 is fixedly installed on the centrifugal base 6, and also comprises a placement plate 14, the placement plate 14 is slidably installed inside the placement box 4, a plurality of placement slots 9 arranged at equal distances are provided on the placement plate 14, which are used to place and store test tubes containing cells to be centrifuged, a support plate 8 is fixedly installed at the bottom of the placement box 4, a magnifying glass 11 is slidably installed on one side of the support plate 8, the magnifying glass 11 is slidably connected to the placement box 4, and an auxiliary mechanism for driving the placement plate 14 to vibrate is provided on the placement box 4, which is used to remove air bubbles between cells in the test tube, so as to prevent the air bubbles after centrifugation from adhering to the cells, causing the cells to be unable to settle, thereby affecting the separation effect;

[0027] Among them, the driving mechanism is arranged in the placement box 4, and is used to drive the placement plate 14 to move, so as to facilitate the observation of the sediment shape of the separated cells and decide whether to continue centrifugation.

[0028] like Figure 2-3As shown, the present embodiment also includes an auxiliary mechanism, which includes an extrusion block 7 fixedly mounted on one side of a placement plate 14, a connecting column 16 fixedly mounted on one side of the placement plate 14, the connecting column 16 is slidably connected to the extrusion block 7, a spring 15 is sleeved on the connecting column 16, one end of the spring 15 is fixedly connected to the bottom of the extrusion block 7, a connecting plate 10 is fixedly mounted on the other side of the placement plate 14, the connecting plate 10 is slidably connected to the placement box 4, the connecting plate 10 is pressed, the connecting plate 10 drives the placement plate 14 to move downward, the extrusion block 7 at one end of the placement plate 14 compresses the spring 15, the spring 15 is deformed to generate elastic force, and the placement plate 14 rebounds, thereby driving the test tube in the placement slot 9 to vibrate and remove air bubbles.

[0029] like Figure 3-5 As shown, the present embodiment also includes a driving mechanism, which includes a connecting block 24 fixedly mounted on the bottom of the support plate 8, the connecting block 24 is arranged in a section, a fixing frame 20 is fixedly mounted on one side of the placement box 4, a threaded rod 19 is threadedly mounted on the fixing frame 20, an extrusion column 25 is rotatably mounted on one end of the threaded rod 19, the extrusion column 25 is slidably connected to the placement box 4, a rotating block 21 is fixedly mounted on one end of the threaded rod 19, when centrifugation is completed, the rotating block 21 is rotated, and the rotating block 21 drives the threaded rod 19 to rotate, because the threaded rod 19 is threadedly connected to the fixing frame 20, with the rotation of the threaded rod 19, the extrusion column 25 at one end of the threaded rod 19 moves inward, squeezes the bottom surface of the connecting block 24, and drives the placement plate 14 to move upward, so that the magnifying glass 11 on one side of the support plate 8 is moved out of the placement box 4 together, so as to facilitate the observation of cells in the test tube after centrifugation.

[0030] like Figure 2-3 As shown, a protrusion 13 is fixedly installed on one side of the support plate 8, a telescopic rod 12 is fixedly installed on the protrusion 13, the telescopic rod 12 is fixedly connected to the magnifying glass 11, a guide groove 23 is opened on the inner wall of the placement box 4, the protrusion 13 is located in the guide groove 23, a limit block 18 is fixedly installed on the bottom of the connecting plate 10, a limit rod 17 is slidably installed on one side of the placement box 4, and one end of the limit rod 17 is located in the limit block 18.

[0031] like Figure 1-4 As shown, a cylinder 2 is fixedly installed on the main body 1, and a slide plate 3 is fixedly installed on one end of the piston rod of the cylinder 2 that passes through the main body 1. The slide plate 3 is slidably connected to the main body 1, and a heat preservation cover 5 is fixedly installed on the bottom of the slide plate 3. When the cylinder 2 is started, the piston rod of the cylinder 2 drives the slide plate 3 to move downward, so that the heat preservation cover 5 can keep the placement box 4 warm. A slide groove 22 is opened on the placement box 4, and the magnifying glass 11 is located in the slide groove 22.

[0032] Working principle: put the test tube containing the cells to be centrifuged into the placement groove 9, press the connecting plate 10, the connecting plate 10 drives the placement plate 14 to move downward, and the extrusion block 7 at one end of the placement plate 14 compresses the spring 15, the spring 15 deforms to generate elastic force, and rebounds the placement plate 14, thereby driving the test tube in the placement groove 9 to vibrate, and the air bubble is removed, and the cylinder 2 is started. The piston rod of the cylinder 2 drives the slide plate 3 to move downward, so that the heat preservation cover 5 keeps the placement box 4 warm. After the centrifugation is completed, the rotating block 21 is rotated, and the rotating block 21 drives the threaded rod 19 to rotate. Since the threaded rod 19 is threadedly connected to the fixing frame 20, as the threaded rod 19 rotates, the extrusion column 25 at one end of the threaded rod 19 moves inward, squeezes the bottom surface of the connecting block 24, and drives the placement plate 14 to move upward, so that the magnifying glass 11 on one side of the support plate 8 is moved out of the placement box 4 together, which is convenient for observing the cells in the test tube after centrifugation, and realizes the removal of air bubbles between the cells before centrifugation, which is convenient for observing the precipitation of cells after the centrifugation, improves the centrifugal effect, and is convenient for operation.

[0033] The above-mentioned specific embodiments are only several optional embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A tissue stem cell separation centrifuge, comprising a main body (1), a centrifuge base (6) fixedly mounted in the main body (1), a placement box (4) fixedly mounted on the centrifuge base (6), characterized in that: Also includes: A placement plate (14), the placement plate (14) being slidably mounted inside the placement box (4), the placement plate (14) being provided with a plurality of placement grooves (9) arranged at equal distances, a support plate (8) being fixedly mounted on the bottom of the placement box (4), a magnifying glass (11) being slidably mounted on one side of the support plate (8), the magnifying glass (11) being slidably connected to the placement box (4), and an auxiliary mechanism for driving the placement plate (14) to vibrate is provided on the placement box (4); A driving mechanism is arranged in the placement box (4) and is used to drive the placement plate (14) to move.

2. A tissue stem cell separation centrifuge according to claim 1, characterized in that: The auxiliary mechanism comprises an extrusion block (7) fixedly mounted on one side of a placement plate (14); a connecting column (16) fixedly mounted on one side of the placement plate (14); the connecting column (16) is slidably connected to the extrusion block (7); a spring (15) is sleeved on the connecting column (16); one end of the spring (15) is fixedly connected to the bottom of the extrusion block (7); a connecting plate (10) is fixedly mounted on the other side of the placement plate (14); the connecting plate (10) is slidably connected to the placement box (4).

3. A tissue stem cell separation centrifuge according to claim 1, characterized in that: The driving mechanism comprises a connecting block (24) fixedly mounted on the bottom of the supporting plate (8), the connecting block (24) being arranged in a cross-section, a fixing frame (20) being fixedly mounted on one side of the placement box (4), a threaded rod (19) being threadedly mounted on the fixing frame (20), an extrusion column (25) being rotatably mounted on one end of the threaded rod (19), the extrusion column (25) being slidably connected to the placement box (4), and a rotating block (21) being fixedly mounted on one end of the threaded rod (19).

4. A tissue stem cell separation centrifuge according to claim 1, characterized in that: A convex block (13) is fixedly mounted on one side of the support plate (8), a telescopic rod (12) is fixedly mounted on the convex block (13), the telescopic rod (12) is fixedly connected to the magnifying glass (11), and a guide groove (23) is formed on the inner wall of the placement box (4), and the convex block (13) is located in the guide groove (23).

5. A tissue stem cell separation centrifuge according to claim 2, characterized in that: A limit block (18) is fixedly mounted on the bottom of the connecting plate (10), and a limit rod (17) is slidably mounted on one side of the placement box (4), with one end of the limit rod (17) being located inside the limit block (18).

6. A tissue stem cell separation centrifuge according to claim 1, characterized in that: A cylinder (2) is fixedly mounted on the main body (1); a slide plate (3) is fixedly mounted on one end of a piston rod of the cylinder (2) that passes through the main body (1); the slide plate (3) is slidably connected to the main body (1); and a heat preservation cover (5) is fixedly mounted on the bottom of the slide plate (3).

7. A tissue stem cell separation centrifuge according to claim 1, characterized in that: The placement box (4) is provided with a slide groove (22), and the magnifying glass (11) is located in the slide groove (22).

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