Umbilical cord tissue treatment low-temperature workbench

Abstract

The utility model discloses an umbilical cord tissue treatment low-temperature workbench, which comprises a tissue treatment cup, a battery cover, a sampling cup, a stepless speed change mechanism, a tissue crushing assembly and a sample collecting assembly, the tissue crushing assembly is arranged below the battery cover, and the sample collecting assembly is arranged at the center of an inner cavity of the tissue treatment cup. According to the low-temperature worktable for treating the umbilical cord tissues, the tissues are prevented from being in contact with the outside through sealing treatment, pollution is avoided, the tissues can be cut into ideal sizes by adjusting different speeds, cells can be conveniently extracted, the collected tissue blocks can be uniformly crushed, and all the tissues required by a test are treated at one time through a large-capacity tissue treatment cup; tissue blocks sheared from a placenta can be directly placed in the tissue treatment cup, the situation that the tissue blocks are clamped back and forth is avoided, hematopoietic stem cells are filtered and collected in time in the crushing process of the sample collection assembly, the cells are prevented from contaminating the inner wall of the tissue treatment cup in the collection process, and the collection efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of umbilical cord tissue processing, in particular to a low-temperature workbench for umbilical cord tissue processing. Background Art

[0002] Stem cells, also known as origin cells and universal cells, are a group of primitive cells with the potential for self-renewal, high proliferation and multidirectional differentiation. They are progenitor cells that can differentiate into various tissues and organs. Hematopoietic stem cells (HSCs) are adult stem cells in the blood system. They are a heterogeneous group with the ability to self-renew for a long time and the potential to differentiate into various mature blood cells. It is the type of adult stem cell with the longest and most in-depth research history.

[0003] For example, the patent document with application number 202122482891.7 discloses an umbilical cord stem cell extraction device, including a bright body, an umbilical cord placement plate is provided on the lower surface of the shell, a leakage hole is penetrated through the surface of the umbilical cord placement plate, a threaded column is penetrated and rotatably connected to the upper surface of the bright body, a threaded driving column is threadedly connected to the surface of the threaded column, the threaded driving column is rotatably connected in the shell, a limiting sliding rod is fixedly connected to the upper surface of the extrusion plate, a sliding groove is provided in the shell, the limiting sliding rod slides on the inner wall of the sliding groove, a spring 1 is fixedly connected to the lower surface of the limiting sliding rod, the lower surface of the spring 1 is fixedly connected to the lower inner wall of the sliding groove, a crushing column is fixedly connected to the lower surface of the extrusion plate, and the surface of the crushing column is slidably connected to the inner wall of the leakage hole: a through groove is provided on the upper surface of the supporting leg, and a container connection mechanism is provided in the through groove, so that there is no need to squeeze the umbilical cord hard when crushing it, the extraction process is more labor-saving, and the extraction efficiency is effectively improved.

[0004] Similar extraction devices rely on manual labor to crush the umbilical cord tissue, which is a time-consuming and labor-intensive process that is prone to contamination. In addition, due to differences in manual operations, the sizes of the processed tissue fragments vary. When sifted later, the hematopoietic stem cells cannot be completely released, affecting the efficiency of cell extraction. Because the placental tissue is relatively large, only a small part can be processed each time during manual processing. Usually, a large number of consumable containers are required to hold the tissue to be processed to process the placental tissue once. When shredding, the shredding can only be performed in sequence. In addition, after shredding, it must be moved to a fine sieve for sieving. The experiment involves multiple tissue transfers, which increases the risk of tissue falling and contamination. When sieving, because the bottom area of ​​the fine sieve is large and the tissue dispersion range is large, a large part of the hematopoietic stem cells obtained by filtration are contaminated by the fine sieve and the inner wall of the collection cup, reducing the collection efficiency.

[0005] Therefore, in view of this, the existing structural deficiencies are studied and improved, and a low-temperature workbench for umbilical cord tissue processing is proposed. Utility Model Content

[0006] The utility model aims to provide a low-temperature workbench for umbilical cord tissue processing. As the use time of the battery increases, the chemical components inside the battery will gradually decompose, resulting in electrolyte leakage. At the same time, if the battery is overcharged, the electrolyte may expand and leak due to overheating. The leakage of electrolyte will contaminate the cabin. When pushing and pulling the battery, the battery will contact with the bracket, and friction will be generated during movement, resulting in push-pull resistance, causing the inconvenience of pushing and pulling.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a low-temperature workbench for umbilical cord tissue processing, comprising a tissue processing cup, a battery cover, a sampling cup, a stepless speed change mechanism, a tissue crushing assembly and a sample collection assembly, wherein the inner surface of the upper end surface of the tissue processing cup is provided with an upper end internal thread, the battery cover is arranged above the tissue processing cup, a connecting rod insertion port is provided at the center of the lower end surface of the battery cover, and an upper end external thread is provided at the edge of the lower end surface of the battery cover, the sampling cup is arranged below the tissue processing cup, and a lower end external thread is provided at the edge of the upper end surface of the sampling cup, a lower end internal thread is provided on the inner surface of the lower end of the tissue processing cup, the stepless speed change mechanism is arranged at the bottom end surface of the inner cavity of the battery cover, and a motor is arranged at the upper end of the stepless speed change mechanism, the tissue crushing assembly is arranged below the battery cover, the sample collection assembly is arranged at the center of the inner cavity of the tissue processing cup, a stepless speed change knob is provided at the center of the front end of the battery cover, and a semiconductor refrigeration sheet is provided in the inner cavity of the bottom end surface of the sampling cup.

[0008] Furthermore, the tissue fragmentation assembly includes a connecting rod and a tissue processing blade. The lower end surface of the battery cover is provided with a connecting rod, and the outer surface of the connecting rod is provided with a tissue processing blade. The tissue processing blade is provided in three groups.

[0009] Furthermore, the sample collection assembly includes a funnel, a filter and a snap-in groove. The funnel is arranged at the center of the inner cavity of the tissue processing cup. The upper end of the funnel is provided with a filter. The snap-in groove is opened at the center of the inner surface of the tissue processing cup.

[0010] Furthermore, an anticoagulant is provided in the inner cavity of the sampling cup, a switch button is provided on the left side of the stepless speed-changing knob, and a speed indicator bar is provided below the stepless speed-changing knob.

[0011] Furthermore, the threads of the upper end internal thread and the upper end external thread are meshed with each other, and the threads of the lower end external thread and the lower end internal thread are meshed with each other.

[0012] Furthermore, the continuously variable transmission mechanism is connected to the upper end of the connecting rod by penetrating the connecting rod insertion port, and the positions of the three groups of tissue processing blades are arranged in an equidistant manner.

[0013] Furthermore, the diameters of the filter screen and the clamping groove are equal, and the outer surface of the filter screen abuts against the inner surface of the clamping groove.

[0014] Compared with the prior art, the utility model has the following beneficial effects: the sealing treatment prevents the tissue from contacting with the outside world and contamination, thereby saving manpower, time and effort; by adjusting different speeds, the tissue can be cut into pieces to an ideal size, which is convenient for cell extraction, and the collected tissue blocks can be evenly broken; all tissues required for the test can be processed at one time through a large-capacity tissue processing cup; the tissue blocks cut from the placenta can be directly placed in the tissue processing cup to avoid clamping the tissue blocks back and forth; and then the sample collection component is used to filter and collect the hematopoietic stem cells in time during the crushing process, so as to prevent the cells from contaminating the inner wall of the tissue processing cup during the collection process, thereby improving the collection efficiency. The specific contents are as follows:

[0015] A tissue processing cup, a battery cover, a sampling cup, a motor, a stepless speed change mechanism and a tissue crushing assembly are provided. The tissue processing cup, the battery cover and the sampling cup are all connected by threads, which effectively isolates the tissue from the outside world, avoids contamination, and saves time and energy. Compared with the traditional manual processing of placental tissue, this is convenient for keeping the tissue clean and for subsequent cell extraction operations. By adjusting the speed, the minced meat can be achieved to the desired ideal size, and the motor drives the stepless speed change mechanism to work, and drives the connecting rod in the tissue crushing assembly to rotate, thereby driving the tissue processing blade to crush the umbilical cord tissue, and adjusting the speed by the stepless speed change mechanism can ensure that the collected tissue blocks are evenly crushed, improve the processing efficiency and quality, and through the large-capacity tissue processing cup, it can accommodate more tissue samples, and process all the tissues required for the test at one time, which can greatly save the operator's time and energy, improve the processing efficiency, and do not require multiple batch processing, reducing the time waste between operations. When cutting the umbilical cord tissue, you only need to put the umbilical cord tissue directly into the tissue processing cup, which simplifies the operation process, reduces the operation steps and human interference, and avoids the possibility of falling when manually clamping the tissue block. Because each time a tissue block is clamped, there is a possibility of contaminating other tissue samples. Placing it directly into the processing cup can effectively reduce the risk of cross contamination and ensure the reliability of the experiment.

[0016] A sample collection component is provided. The edge of the filter is made of plastic and has a certain flexibility. When the filter and the funnel are placed in the filter, the edge of the filter is squeezed into the tissue processing cup and is limited by abutting against the card slot to fix the filter. Then the filter can effectively separate the target cells, and the funnel can quickly flow the separated cells into the sampling cup at the lower end, so that the hematopoietic stem cells can be quickly filtered and collected into the sampling cup during the crushing process, which can prevent the cells from contaminating the inner wall of the tissue processing cup during the collection process. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the front section structure of a low-temperature workbench for umbilical cord tissue processing according to the utility model;

[0018] Figure 2 It is a three-dimensional exploded structural schematic diagram of a low-temperature workbench for umbilical cord tissue processing according to the utility model;

[0019] Figure 3 The utility model is a low temperature workbench for umbilical cord tissue processing Figure 2 A local enlarged structural diagram of the part;

[0020] Figure 4 The utility model is a three-dimensional structural schematic diagram of a tissue crushing component of a low-temperature workbench for umbilical cord tissue processing.

[0021] In the figure: 1. tissue processing cup; 2. battery cover; 21. connecting rod insertion port; 3. sampling cup; 31. anticoagulant; 4. motor; 5. continuously variable speed mechanism; 6. tissue crushing assembly; 601. connecting rod; 602. tissue processing blade; 7. sample collection assembly; 701. filter screen; 702. funnel; 703. snap-in slot; 8. continuously variable speed knob; 9. switch button; 10. speed indicator bar; 11. semiconductor cooling plate; 12. upper end internal thread; 13. upper end external thread; 14. lower end external thread; 15. lower end internal thread. DETAILED DESCRIPTION

[0022] The following is a further detailed description of the implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0023] like Figures 1 to 4As shown, a low-temperature workbench for umbilical cord tissue processing comprises a tissue processing cup 1, a battery cover 2, a sampling cup 3, a continuously variable transmission mechanism 5, a tissue crushing assembly 6 and a sample collection assembly 7. The inner surface of the upper end surface of the tissue processing cup 1 is provided with an upper end internal thread 12. The tissue processing cup 1 is a container for accommodating and processing umbilical cord tissue. The battery cover 2 is arranged above the tissue processing cup 1. The battery cover 2 provides a power driving function for the transmission system. The edge of the lower end surface of the battery cover 2 is provided with an upper end external thread 13. The threads of the upper end internal thread 12 and the upper end external thread 13 are meshed with each other. The tissue processing cup 1 is meshed with the upper end external thread 13 of the battery cover 2 through the upper end internal thread 12, thereby sealing it and ensuring the sealing performance of the upper end of the tissue processing cup 1. The sampling cup 3 is arranged below the tissue processing cup 1. The sampling cup 3 is used to collect the processed sample, and the edge of the upper end surface of the sampling cup 3 is provided with A lower end external thread 14 is provided, and a lower end internal thread 15 is provided on the inner surface of the lower end of the tissue processing cup 1. The threads of the lower end external thread 14 and the lower end internal thread 15 are meshed with each other. The sampling cup 3 is meshed with the lower end internal thread 15 of the tissue processing cup 1 through the lower end external thread 14, so as to connect and seal them. The threaded connection between the tissue processing cup 1, the battery cover 2 and the sampling cup 3 ensures the overall sealing effect, thereby preventing sample leakage and external contamination. The inner cavity of the bottom end surface of the sampling cup 3 is provided with a semiconductor refrigeration sheet 11. The semiconductor refrigeration sheet 11 adjusts the cooling effect by controlling the size and direction of the current to ensure a constant low temperature state of the sample and maintain its stability. The inner cavity of the sampling cup 3 is provided with an anticoagulant 31. The anticoagulant 31 is a chemical substance used to prevent blood from clotting in the sampling cup 3, thereby maintaining the liquid state of the sample and ensuring the integrity and properties of the sample.

[0024] A continuously variable transmission mechanism 5 is arranged on the bottom end surface of the inner cavity of the battery cover 2, and a motor 4 is arranged on the upper end of the continuously variable transmission mechanism 5. The continuously variable transmission mechanism 5 controls and adjusts the rotation speed and strength of the tissue crushing component 6 connected thereto through the motor 4, thereby realizing fine adjustment of the tissue crushing process. A continuously variable transmission knob 8 is arranged at the center of the front end of the battery cover 2, and a speed indicator bar 10 is arranged below the continuously variable transmission knob 8. The speed indicator bar 10 can clearly display the current working speed of the equipment and provide the reference information required by the operator. The rotation speed of the continuously variable transmission mechanism 5 can be adjusted by rotating the continuously variable transmission knob 8. A switch button 9 is arranged on the left side of the continuously variable transmission knob 8. The switch button 9 is used to control the start and stop function of the equipment. The tissue crushing component 6 is arranged below the battery cover 2. The crushing assembly 6 includes a connecting rod 601 and a tissue processing blade 602. The connecting rod 601 is provided on the lower end surface of the battery cover 2. A connecting rod insertion port 21 is provided at the center of the lower end surface of the battery cover 2. The connecting rod 601 is connected to the lower end of the continuously variable transmission mechanism 5 by penetrating the connecting rod insertion port 21. The connecting rod 601 is locked after being inserted through the connecting rod insertion port 21 and can be removed when the work is completed. The outer surface of the connecting rod 601 is provided with a tissue processing blade 602. The tissue processing blade 602 is provided in three groups. The positions of the three groups of tissue processing blades 602 are arranged in an equidistant manner. The three groups of tissue processing blades 602 are connected to the outer surface of the connecting rod 601. With the adjustment of the continuously variable transmission mechanism 5, they rotate and crush the umbilical cord tissue sample to achieve the purpose of tissue decomposition;

[0025] The sample collection component 7 is arranged at the center of the inner cavity of the tissue processing cup 1. The sample collection component 7 includes a funnel 701, a filter screen 702 and a snap-in groove 703. The upper end of the funnel 701 is provided with a filter screen 702. After the tissue is broken, the hematopoietic stem cells therein are filtered and collected in time through the filter screen 702. The funnel 701 is arranged at the center of the inner cavity of the tissue processing cup 1. The cells flow into the sampling cup 3 through the funnel 701 to prevent the cells from contaminating the inner wall of the tissue processing cup 1 during the collection process, thereby improving the collection efficiency. A snap-in groove 703 is provided at the center of the inner surface of the tissue processing cup 1. The diameters of the filter screen and the snap-in groove 703 are equal. The outer surface of the filter screen 702 is in contact with the inner surface of the snap-in groove 703. The snap-in groove 703 is used to fix and connect the filter screen 702 to ensure that the filter screen 702 can be firmly fixed in the center of the inner cavity of the tissue processing cup 1 without displacement.

[0026] In summary, if Figures 1 to 4As shown, the umbilical cord tissue processing low-temperature workbench, the tissue processing cup 1 is installed by meshing the upper end internal thread 12 with the upper end external thread 13 of the battery cover 2, and then the sampling cup 3 is meshed and connected with the lower end internal thread 15 of the tissue processing cup 1 by the lower end external thread 14, so as to form a sealed whole. The operator starts the device by pressing the switch button 9, and adjusts the speed of the continuously variable speed mechanism 5 by rotating the continuously variable speed knob 8. The continuously variable speed mechanism 5 controls the speed and strength of the tissue crushing component 6 connected thereto through the motor 4, so as to achieve fine adjustment of the tissue crushing process. The connecting rod 601 is inserted through the connecting rod insertion port 21 and locked, and can be removed when the work is completed. The tissue processing blade 602 is connected to the outer surface of the connecting rod 601, and rotates and crushes the umbilical cord tissue sample with the adjustment of the continuously variable speed mechanism 5, so as to achieve the purpose of tissue decomposition;

[0027] The edge of the filter 702 in the sample collection component 7 is made of plastic material and has a certain degree of flexibility. When the filter 702 and the connected funnel 701 are placed, the edge of the filter 702 is squeezed into the tissue processing cup and is limited by abutting against the snap-in groove 703, thereby fixing the filter 703. When it needs to be cleaned, it can be removed by pressing or pushing. After the umbilical cord tissue is broken, the hematopoietic stem cells therein are filtered and collected in time through the filter 702, and flow through the funnel to the inner cavity of the sampling cup 3 below, where the hematopoietic stem cells are mixed and reacted with the anticoagulant 31, thereby maintaining the liquid state of the sample, and passing the current through the semiconductor material of the semiconductor refrigeration sheet 11, the side that generates heat becomes hot and the other side becomes cold, and the heat is absorbed from the sample to the refrigeration sheet through heat conduction and convection, thereby reducing the temperature of the sample.

Claims

1. A low-temperature workbench for umbilical cord tissue processing, comprising a tissue processing cup (1), a battery cover (2), a sampling cup (3), a stepless speed change mechanism (5), a tissue fragmentation component (6) and a sample collection component (7), characterized in that: The inner surface of the upper end surface of the tissue processing cup (1) is provided with an upper end internal thread (12); the battery cover (2) is arranged above the tissue processing cup (1); a connecting rod insertion port (21) is provided at the center of the lower end surface of the battery cover (2); and an upper end external thread (13) is provided at the edge of the lower end surface of the battery cover (2); the sampling cup (3) is arranged below the tissue processing cup (1); and a lower end external thread (14) is provided at the edge of the upper end surface of the sampling cup (3); The inner surface is provided with a lower internal thread (15); the stepless speed change mechanism (5) is arranged at the bottom end surface of the inner cavity of the battery cover (2); and a motor (4) is arranged at the upper end of the stepless speed change mechanism (5); the tissue fragmentation component (6) is arranged below the battery cover (2); the sample collection component (7) is arranged at the center of the inner cavity of the tissue processing cup (1); a stepless speed change knob (8) is arranged at the center of the front end of the battery cover (2); and a semiconductor cooling plate (11) is arranged in the inner cavity of the bottom end surface of the sampling cup (3).

2. The umbilical cord tissue processing low temperature workbench according to claim 1, characterized in that: The tissue fragmentation assembly (6) comprises a connecting rod (601) and a tissue processing blade (602); the lower end surface of the battery cover (2) is provided with a connecting rod (601); the outer surface of the connecting rod (601) is provided with a tissue processing blade (602); and the tissue processing blade (602) is provided in three groups.

3. The umbilical cord tissue processing low temperature workbench according to claim 1, characterized in that: The sample collection component (7) comprises a funnel (701), a filter screen (702) and a snap-in groove (703); the funnel (701) is arranged at the center of the inner cavity of the tissue processing cup (1); the filter screen (702) is arranged at the upper end of the funnel (701); and the snap-in groove (703) is opened at the center of the inner surface of the tissue processing cup (1).

4. The umbilical cord tissue processing low temperature workbench according to claim 1, characterized in that: The inner cavity of the sampling cup (3) is provided with an anticoagulant (31), a switch button (9) is provided on the left side of the stepless speed change knob (8), and a speed indicator bar (10) is provided below the stepless speed change knob (8).

5. The umbilical cord tissue processing low temperature workbench according to claim 1, characterized in that: The threads of the upper end internal thread (12) and the upper end external thread (13) are meshed with each other, and the threads of the lower end external thread (14) and the lower end internal thread (15) are meshed with each other.

6. The umbilical cord tissue processing low temperature workbench according to claim 2, characterized in that: The connecting rod (601) is connected to the lower end of the stepless speed change mechanism (5) by penetrating the connecting rod insertion port (21), and the positions of the three groups of tissue processing blades (602) are arranged in an equidistant manner.

7. The umbilical cord tissue processing low temperature workbench according to claim 3, characterized in that: The diameters of the filter screen (702) and the clamping groove (703) are equal, and the outer surface of the filter screen (702) abuts against the inner surface of the clamping groove (703).

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