Wafer cartridge for biochip detection

Abstract

The utility model provides a wafer cartridge for biochip detection, and aims to solve the problem of leakage caused by a gap between the end surfaces of an upper-layer cartridge and a lower-layer cartridge of a traditional biochip cartridge. The device mainly comprises an upper-layer cartridge, a lower-layer cartridge, a micro-channel device and a biochip assembly, a new scheme is provided for solving the problem of cartridge micro-channel leakage, the silica gel gasket with the thickness larger than 10% of the gap is added between the upper cartridge and the lower cartridge, and medium leakage is prevented through pressing force. Meanwhile, the clamping box layer is connected through the UV biological glue so that the sealing effect can be further enhanced. In order to solve the problems of the size and blockage of the flow channel, the width of the micro-flow channel is set to be 1.5 mm, and 12 medicine containing cavities with the depth of 1.0 mm are embedded to be filled with medicine, so that compared with a traditional flow channel of dozens of microns to hundreds of microns, the micro-flow channel is wider, and the blockage of the micro-flow channel caused by molecular aggregation or blood clot aggregation can be more effectively prevented in combination with medicine pre-embedding.

Description

Technical Field

[0001] The utility model relates to the technical field of biological chip detection, in particular to a chip cartridge used for biological chip detection. Background Art

[0002] Microfluidic chip is also commonly called biochip. Microfluidic technology can integrate the whole experimental process and functions of conventional biochemical analysis, such as sampling, dilution, reagent addition, reaction, separation, and detection, on a small solid material such as silicon, glass, plastic or metal, forming a microchip including a variety of micro-nano channels and multiple micro-nanoliter reaction chambers, with controllable fluid running through the whole system for clinical pathology detection.

[0003] The main component of a biochip is the biochip cassette inside it. The structure of an existing biochip cassette is specifically manifested in an upper cassette, a lower cassette, and a microfluidic channel and a biochip carrier arranged on the lower cassette. In the microfluidic channel design, since the microfluidic channel is designed on the surface of the lower cassette, there is a gap between the end faces of the upper cassette and the lower cassette, which will cause leakage at the joint between the upper and lower layers, thereby affecting the detection accuracy or directly causing the detection work to be unable to proceed downward. Utility Model Content

[0004] In view of the shortcomings of the above-mentioned prior art, the present application provides a chip cassette for biochip detection, which prevents leakage of the medium by adding a silicone gasket with a thickness exceeding 10% of the gap between the upper and lower cassettes and using the pressing force. At the same time, UV bio-glue is used to connect the cassette layers to further enhance the sealing effect. Regarding the flow channel size and clogging issues, the present invention sets the microchannel width to 1.5mm and buries 12 holes with a depth of 1.0mm to fill in the medicine. Compared with the traditional flow channels of tens to hundreds of um, it is more spacious, and combined with the pre-embedded medicine, it effectively avoids the occurrence of clogging.

[0005] The technical solution adopted by the utility model is as follows:

[0006] A chip cassette for biochip detection comprises an upper cassette and a lower cassette, wherein a microfluidic device is arranged on the surface of the lower cassette, a biochip assembly is detachably arranged on the bottom of the lower cassette, a silicone gasket is sandwiched between the end face of the lower cassette relative to the end face of the upper cassette, and the four peripheral edges of the lower cassette relative to the upper cassette are bonded by glue dispensing.

[0007] Furthermore, after the upper cartridge is combined with the lower cartridge, a gap exists between the microfluidic device and the lower surface of the upper cartridge, and the thickness of the silicone gasket is greater than the height of the gap.

[0008] Furthermore, the lower layer cassette is provided with glue grooves around its periphery, and the upper layer cassette is correspondingly provided with convex grooves around its periphery, and the lower layer cassette is fixedly connected to the upper layer cassette by dispensing glue in the glue grooves.

[0009] Furthermore, the microfluidic device comprises a sample medium flow channel and a pre-test flow channel, and both the sample medium flow channel and the pre-test flow channel flow down to the biochip assembly below through an inlet.

[0010] Furthermore, the sample medium flow channel includes a first pore cavity, a medium cavity, a microfluidic channel, a filter paper cavity and an inlet in sequence from the inlet end to the terminal end, and a plurality of drug cavities are evenly distributed along the path of the microfluidic channel.

[0011] Furthermore, the pre-test channel includes a second air hole cavity, a test liquid containing cavity and an introduction port in sequence from the inlet end to the terminal end.

[0012] Furthermore, the surface of the upper cartridge is provided with a first air hole, a medium hole and a second air hole corresponding to the microfluidic device.

[0013] Furthermore, the biochip assembly includes a biochip carrier detachably mounted at the bottom of the lower cartridge, the biochip carrier being provided with a left chamber and a right chamber interconnected with each other, the left chamber being provided with liquid absorbing foam, and the right chamber being provided with a biochip body.

[0014] Furthermore, the width of the microfluidic channel is 1.5 mm.

[0015] The beneficial effects of the utility model are as follows:

[0016] Compared with the prior art, in order to solve the problem of leakage in the microchannel of the cassette, the utility model adds a layer of silicone gasket between the upper cassette and the lower cassette, and uses the force after the upper and lower layers are pressed together to prevent the leakage of the medium in the microchannel. In particular, the thickness of the gasket designed in the utility model is 10% greater than the thickness of the gap between the layers, so that the gasket can be extended after being compressed, further compensating the end face gap, thereby improving the sealing effect of the microchannel, and injecting UV biological glue at the edge of the cassette to connect the upper and lower cassette layers. The strong bonding between the upper and lower layers provides further compression force for the silicone gasket to solve the problem of interlayer leakage.

[0017] Compared with the existing technology, in terms of channel size and clogging, due to the characteristics of the microchannel, the limitation of channel size may become a challenge for processing larger samples or realizing certain chemical reactions. The utility model sets the width of the microchannel to 1.5mm, and buries 12 holes with a depth of 1.0mm in the microchannel through which the whole blood sample flows to fill Anti-D or other drugs. The width of 1.5mm is more spacious than the traditional tens to hundreds of um, so it can more effectively prevent the microchannel from being blocked due to molecular aggregation or blood clot aggregation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional schematic diagram of the utility model from a first perspective;

[0019] Figure 2 It is a stereoscopic schematic diagram of the second viewing angle of the utility model;

[0020] Figure 3 It is an exploded view of the utility model;

[0021] Figure 4 It is a schematic diagram of the structure of the microfluidic device in the utility model;

[0022] Figure 5 It is a schematic diagram of the structure of the biochip assembly in the utility model.

[0023] Among them: 1. upper cartridge; 2. lower cartridge; 4. biochip assembly; 5. silicone gasket; 6. glue slot; 8. sample medium flow channel; 9. pre-inspection flow channel; 10. introduction port; 11. first air hole; 12. medium hole; 13. second air hole;

[0024] 41. Biochip carrier; 42. Left chamber; 43. Right chamber; 44. Liquid-absorbing foam; 45. Biochip body;

[0025] 81. first pore cavity; 82. medium cavity; 83. microfluidic channel; 84. filter paper cavity; 85. drug cavity;

[0026] 91. Second air hole cavity; 92. Detection liquid containing cavity. DETAILED DESCRIPTION

[0027] The specific implementation of the utility model is described below in conjunction with the accompanying drawings.

[0028] The utility model provides a chip cartridge for biological chip detection, aiming to solve the leakage problem of the traditional biological chip cartridge caused by the gap between the end faces of the upper cartridge 1 and the lower cartridge 2.

[0029] like Figures 1 to 5As shown, the utility model includes an upper cartridge 1 and a lower cartridge 2, the surface of the lower cartridge 2 is provided with a microfluidic device, the bottom of the lower cartridge 2 is detachably provided with a biochip assembly 4, a silicone gasket 5 is sandwiched between the end face of the lower cartridge 2 relative to the end face of the upper cartridge 1, and the four peripheral edges of the lower cartridge 2 relative to the upper cartridge 1 are bonded by glue dispensing.

[0030] In one embodiment of the present invention, after the upper cartridge 1 is combined with the lower cartridge 2 , a gap exists between the microfluidic device and the lower surface of the upper cartridge 1 , and the thickness of the silicone gasket 5 is greater than the height of the gap.

[0031] In one embodiment of the utility model, glue grooves 6 are provided around the lower layer cassette 2 , and convex grooves are correspondingly provided around the upper layer cassette 1 . The lower layer cassette 2 is fixed to the upper layer cassette 1 by dispensing glue in the glue grooves 6 .

[0032] In one embodiment of the present invention, the microfluidic device includes a sample medium flow channel 8 and a pre-test flow channel 9 , and both the sample medium flow channel 8 and the pre-test flow channel 9 flow down to the biochip assembly 4 below through an inlet 10 .

[0033] In one embodiment of the utility model, the sample medium flow channel 8 includes a first pore cavity 81, a medium cavity 82, a microfluidic channel 83, a filter paper cavity 84 and an inlet 10 from the inlet end to the end, and a plurality of drug cavities 85 are evenly distributed along the path of the microfluidic channel 83.

[0034] In one embodiment of the present invention, the pre-test channel 9 includes a second air hole cavity 91 , a test liquid chamber 92 and an inlet 10 in sequence from the inlet end to the outlet end.

[0035] In one embodiment of the present invention, a first air hole 11 , a medium hole 12 and a second air hole 13 are disposed on the surface of the upper cartridge 1 corresponding to the micro-channel device.

[0036] In one embodiment of the utility model, the biochip assembly 4 includes a biochip carrier 41 detachably mounted on the bottom of the lower cartridge 2, and a left chamber 42 and a right chamber 43 that are interconnected are distributed on the biochip carrier 41, a liquid-absorbing foam 44 is installed in the left chamber 42, and a biochip body 45 is installed in the right chamber 43.

[0037] In one embodiment of the present invention, the width of the microfluidic channel 83 is 1.5 mm.

[0038] The specific structure and working principle of the utility model:

[0039] The utility model comprises a set of upper cartridges 1 and a set of lower cartridges 2. A first air hole 11, a medium hole 12 and a second air hole 13 are provided on the surface of the upper cartridge 1. The two air holes are used to inject high-pressure gas to promote the flow of the medium in the corresponding microfluidic channel 83. The medium hole 12 is used to inject the detection medium from the outside into the device. In this embodiment, the medium is blood. In other embodiments, it includes but is not limited to this, such as saliva, etc.

[0040] The surface of the lower cartridge 2 is provided with a microfluidic device, which is used to push the blood sample to be tested and the pre-test test solution respectively onto the biochip body 45 at the bottom to cause a chemical reaction;

[0041] The pre-test flow channel 9 includes a second pore cavity 91, a detection liquid chamber 92 and an introduction port 10 from the inlet end to the end. When in use, the detection liquid chamber 92 is filled with a detection liquid used as a reference for diagnosing the test results. The detection liquid flows in the microchannel 83 through the high-pressure gas in the second pore cavity 91, and finally flows into the introduction port 10 to reach the biochip body 45, and reacts chemically with the biochip for the first time to achieve a pre-test reference.

[0042] The sample medium flow channel 8 includes the first pore cavity 81, the medium cavity 82, the microfluidic channel 83, the filter paper cavity 84 and the introduction port 10 from the inlet end to the end, and a plurality of drug cavities 85 are evenly distributed along the path of the microfluidic channel 83. When in use, blood is injected into the medium cavity 82 from the outside, and high-pressure gas is pushed into the first pore cavity 81 from the outside to push the blood to flow in the microfluidic channel 83, and finally converges into the introduction port 10 after passing through the drugs and the filter paper cavity 84 on the path. The introduction port 10 is connected to the biochip body 45 of the lower layer, so as to chemically react with the biochip body 45;

[0043] After the first pre-test flow channel 9, i.e., the test liquid, reacts chemically with the biochip, the sample medium flow channel 8, i.e., the blood sample, intervenes and reacts chemically with the biochip again; at this time, the test liquid in the pre-test flow channel 9 intervenes again, and the test liquid is introduced into the biochip for a third chemical reaction, and the first and third chemical reaction data are collected; at this point, a sample test completes the closed loop and can be used for clinical diagnosis of some parameters, such as diabetes, etc. It should be noted that the specific working principle based on the biochip test is a prior art and will not be repeated here.

[0044] The biochip assembly 4 includes a biochip carrier 41 which is detachably mounted on the bottom of the lower cartridge 2. The biochip carrier 41 is provided with a left chamber 42 and a right chamber 43 which are interconnected. The left chamber 42 is provided with blood-absorbing foam, and the right chamber 43 is provided with a biochip body 45. The waste liquid after chemical reaction in the biochip body 45 will flow through the blood-absorbing foam for centralized recovery.

[0045] The above description is an explanation of the utility model, not a limitation of the utility model. The scope of the utility model is defined by the claims. Any form of modification can be made within the protection scope of the utility model.

Claims

1. A chip cartridge for biochip detection, comprising an upper cartridge (1) and a lower cartridge (2), wherein a microfluidic device is disposed on the surface of the lower cartridge (2), and a biochip assembly (4) is detachably disposed on the bottom of the lower cartridge (2), characterized in that: A silicone gasket (5) is sandwiched between the end surface of the lower layer cartridge (2) and the end surface of the upper layer cartridge (1), and the four peripheral edges of the lower layer cartridge (2) and the upper layer cartridge (1) are bonded by glue.

2. A chip cartridge for biochip testing as claimed in claim 1, characterized in that: After the upper cartridge (1) and the lower cartridge (2) are combined, a gap exists between the microfluidic device and the lower surface of the upper cartridge (1), and the thickness of the silicone gasket (5) is greater than the height of the gap.

3. A chip cartridge for biochip testing as claimed in claim 1, characterized in that: The lower layer cassette (2) is provided with glue grooves (6) around its periphery, and the upper layer cassette (1) is correspondingly provided with convex grooves around its periphery. The lower layer cassette (2) is fixedly connected to the upper layer cassette (1) by dispensing glue in the glue grooves (6).

4. A chip cassette for biochip testing as claimed in claim 1, characterized in that: The microfluidic device comprises a sample medium flow channel (8) and a pre-test flow channel (9), and both the sample medium flow channel (8) and the pre-test flow channel (9) flow to the biological chip assembly (4) below through an inlet (10).

5. A chip cassette for biochip testing as claimed in claim 4, characterized in that: The sample medium flow channel (8) includes, from the inlet end to the outlet end, a first pore cavity (81), a medium cavity (82), a microfluidic channel (83), a filter paper cavity (84) and an inlet (10), and a plurality of drug cavities (85) are evenly distributed along the path of the microfluidic channel (83).

6. A chip cassette for biochip testing as claimed in claim 4, characterized in that: The pre-test flow channel (9) comprises, from the inlet end to the outlet end, a second air hole cavity (91), a test liquid containing cavity (92) and an inlet port (10).

7. A chip cartridge for biochip testing as claimed in claim 1, characterized in that: The surface of the upper cartridge (1) is provided with a first air hole (11), a medium hole (12) and a second air hole (13) corresponding to the microfluidic device.

8. A chip cassette for biochip testing as claimed in claim 1, characterized in that: The biochip assembly (4) includes a biochip carrier (41) which is detachably mounted on the bottom of the lower cartridge (2); the biochip carrier (41) is provided with a left chamber (42) and a right chamber (43) which are interconnected; a liquid-absorbing foam (44) is installed in the left chamber (42); and a biochip body (45) is installed in the right chamber (43).

9. A chip cartridge for biochip testing as claimed in claim 5, characterized in that: The width of the microfluidic channel (83) is 1.5 mm.

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