Portable isothermal amplification and fluorescence visual detection device

Abstract

The utility model discloses a portable isothermal amplification and fluorescence visualization detection device, which comprises a rotating mechanism, a plurality of annularly distributed amplification reaction tanks are arranged on the upper surface of the rotating mechanism, marks are respectively arranged on the surface of the rotating mechanism corresponding to the amplification reaction tanks, and the amplification reaction tanks are respectively provided with corresponding sensors. According to the utility model, the rotating mechanism is arranged, the amplification reaction tank is arranged on the rotating mechanism, the test tube is inserted into the amplification reaction tank to be fixed, the test tube is convenient to take and use through the rotation of the rotating mechanism, the sensor is arranged, the test tube is inserted into the amplification reaction tank, and the printing device is arranged on the main control module. The sensor senses insertion of the test tube, the label is automatically printed by the printing device according to the identification of the inserted amplification reaction tank, after the label is pasted on the test tube, subsequent sample tracking is facilitated, the efficiency is improved, and falling and blurring caused by long-time use of the label are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of test equipment, in particular to a portable constant temperature amplification and fluorescence visualization detection device. Background Art

[0002] In vitro diagnostic (IVD) technology usually needs to be operated by technicians in a professional laboratory environment, which is not only costly but also difficult to implement in some special scenarios (such as remote areas, emergency epidemic sites, or the homes of patients with limited mobility). Existing IVD equipment is usually disposable, resulting in material waste and increased testing costs. In addition, such equipment is usually large in size, inconvenient to carry, and complex to operate, requiring professional training to use, which limits its widespread application in non-professional environments. Especially when the demand for immediate testing is high, the warm-up time and detection cycle of traditional equipment are long, and it is impossible to provide test results quickly, which affects diagnostic efficiency.

[0003] To address these issues, a portable constant-temperature fluorescence amplification analyzer for nucleic acid detection has been developed in the prior art (as shown in patent CN218491750U). This instrument is miniaturized, simplified in structure, and easy to carry. It can perform fluorescence detection on 1 to 4 samples and display the results through an indicator light. It can be paired with a mobile phone or computer via a wireless connection (such as Bluetooth), upload test data, and plot an amplification curve. The analyzer preheats quickly and can complete the test in a short time, making it suitable for immediate testing needs. It is simple to operate and can be used without professional training. It is low in cost and easy to promote.

[0004] However, existing portable analyzers still have some shortcomings. For example, they lack automated mechanisms in sample processing, requiring manual operation and prone to human error. They are also not convenient in sample identification and tracking, which may lead to sample confusion or label detachment. These include:

[0005] Human error: Hand-written labels are prone to errors, especially in a high-intensity work environment. When busy or tired, labels may be misplaced, resulting in sample mix-ups.

[0006] Inefficiency: Each sample needs to be individually labeled, which consumes time and effort. Handwritten labels can be difficult to read, affecting subsequent data processing and experimental results.

[0007] Difficulty in tracking: The lack of a unified coding system makes sample tracking complicated. When the number of samples is large, finding a specific sample becomes very time-consuming.

[0008] Data consistency issues: Manually recorded information may be inconsistent, affecting the accuracy of data analysis. Differences in writing style between different experimenters can also cause difficulties in interpretation.

[0009] Safety issues: Sample labels can easily fall off or become unclear, potentially leading to the misuse of hazardous materials. In certain special environments (such as low-temperature storage), traditional labels may not maintain their integrity.

[0010] These defects and deficiencies can lead to serious consequences, such as deviations in experimental results, delays in research progress, etc. Therefore, there is an urgent need for a more efficient, accurate and reliable automated identification solution.

[0011] To solve the above problems, a portable constant temperature amplification and fluorescence visualization detection device is proposed. Utility Model Content

[0012] The purpose of the present utility model is to provide a portable constant temperature amplification and fluorescence visualization detection device to solve the problems raised in the above-mentioned background technology. This design aims to further improve the function of the portable nucleic acid constant temperature amplification analyzer. By introducing a rotating mechanism, the degree of automation of sample processing is improved, the operating process is simplified, and human errors are reduced. A plurality of amplification reaction pools are provided on the rotating mechanism, and the test tubes can be conveniently accessed through the rotating mechanism after being inserted. Each amplification reaction pool is equipped with a sensor. When the test tube is inserted, the sensor will detect and notify the control system, and automatically print a label according to the placement slot identification, which facilitates sample tracking, improves work efficiency, and avoids the problem of label shedding and blurring caused by long-term use. This improvement makes the portable analyzer more practical and efficient in a variety of application scenarios.

[0013] In order to solve the above technical problems, the present invention is improved on the basis of the existing mechanism (patent CN218491750U). The existing portable nucleic acid constant temperature amplification analyzer mainly includes a shell component, a power supply module, a main control module, a fluorescence detection module, a temperature control module and an amplification reaction pool; after the improvement, the present invention provides the following technical solutions: a portable constant temperature amplification and fluorescence visualization detection device, including a rotating mechanism arranged above the temperature control module, the upper surface of the rotating mechanism is provided with a plurality of amplification reaction pools distributed in a ring shape, the upper surface of the rotating mechanism is respectively provided with markings corresponding to the amplification reaction pools, and corresponding sensors are respectively provided in the amplification reaction pools, the sensors are connected to the main control module, and the main control module is connected to a printing device.

[0014] Specifically, the rotating mechanism is located above the fluorescence detection module, and the fluorescence detection module is located above the temperature control module.

[0015] Specifically, the sensor is connected to the main control module. When the test tube is inserted into the amplification reaction pool, the sensor sends a signal to the main control module to inform the main control module which position of the test tube is ready.

[0016] According to the above technical solution, a sensor is provided on the side wall of each amplification reaction pool for detecting whether a test tube is inserted into the amplification reaction pool.

[0017] Specifically, the main control module is also connected to a printing device. When the test tube is inserted into the amplification reaction pool, the main control module will trigger the printing device to print a corresponding label according to the identification of the test tube position.

[0018] According to the above technical solution, on the basis of the original patent CN218491750U, a rotating mechanism is added, and the rotating mechanism is located above the fluorescence detection module, and the fluorescence detection module is located above the temperature control module.

[0019] Temperature control module: Located at the bottom, it is responsible for maintaining a constant temperature environment. The temperature control module includes a metal heat conducting plate, a heating plate, and thermal insulation foam. These components are described in the original patent CN218491750U and will not be further described in this application.

[0020] Fluorescence Detection Module: Located above and adjacent to the temperature control module, the fluorescence detection module is responsible for detecting the fluorescence signal within the test tube on the rotating mechanism. The fluorescence detection module includes a light source, pre-focusing excitation optical path, an aperture, a first lens, and other components. These components are described in the original patent CN218491750U and are not further detailed in this application.

[0021] According to the above technical solution, a base is provided at the bottom of the rotating mechanism, a connecting column is fixedly connected to the upper surface of the base, and the rotating mechanism is cylindrical.

[0022] According to the above technical solution, a first magnet is provided on the lower surface of the rotating mechanism, and the position of the first magnet corresponds to the amplification reaction pool, and a second magnet is provided on the upper surface of the base to cooperate with the first magnet. The functions of the magnets include: 1. The first magnet and the second magnet attract each other through magnetic force, ensuring that the rotating mechanism can be accurately positioned at a predetermined position during the rotation process. 2. The attractive force of the magnet can also provide a shock-absorbing effect, reducing vibration and shaking during rotation, and ensuring that the test tubes in the amplification reaction pool remain stable during rotation. 3. When the rotating mechanism stops, the attractive force of the magnet can provide additional holding force to prevent accidental movement due to external force or other factors, thereby ensuring the safety and reliability of the device.

[0023] According to the above technical solution, the interior of the rotating mechanism is in direct contact with the amplification reaction pool.

[0024] According to the above technical solution, the inner side walls of the amplification reaction pool are respectively provided with anti-slip sheets.

[0025] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0026] The utility model is provided with a rotating mechanism, on which an amplification reaction pool is arranged, and a test tube is inserted into the amplification reaction pool for fixation. The rotation of the rotating mechanism facilitates the taking out of the test tube. A sensor is provided, and after the test tube is inserted into the amplification reaction pool, the sensor senses the insertion of the test tube. According to the identification of the inserted amplification reaction pool, a printing device automatically prints a label. After the label is affixed to the test tube, subsequent sample tracking is convenient, efficiency is improved, and the label is prevented from falling off and becoming blurred due to long-term use. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0028] Figure 1 It is a structural diagram of the utility model;

[0029] Figure 2 This is a schematic diagram of the main cross-sectional structure of the utility model;

[0030] In the figure: 1-rotating mechanism, 2-amplification reaction pool, 3-base, 4-connecting column, 5-connecting groove, 6-first magnet, 7-second magnet, 8-sensor housing, 9-anti-slip sheet. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0032] A portable constant-temperature amplification analyzer for nucleic acid detection by fluorescence has been disclosed in the prior art (as shown in patent CN218491750U). Its structure has been clearly described in this application and can be summarized as follows: it mainly includes a shell component, a power supply module, a main control module, a fluorescence detection module, a temperature control module and an amplification reaction pool; the shell component is composed of an upper shell, a lower shell, a flip cover, a bottom pad and an operation button, providing structural support and an operation interface for the device; the power supply module powers the device through a power adapter; the main control module includes a PCBA control board, an indicator light strip, a buzzer and a Bluetooth module, which are used for overall control, status display, sound prompts and wireless data transmission of the device; the fluorescence detection module includes a light source, an aperture, a lens, a filter and a silicon photocell PD, which is used to generate excitation light, control the beam angle, focus light, selectively transmit fluorescence and detect fluorescence signals; the temperature control module is composed of a metal heat conduction plate, a heating plate, thermal insulation cotton, etc., which is used to conduct heat, keep warm, monitor and control temperature; the amplification reaction pool is used to place samples, and the number can be adapted to different detection needs.

[0033] In summary, for greater clarity, the relationship between the fluorescence detection module, temperature control module, sensor, and amplification reaction pool is further explained:

[0034] Temperature control module: Located at the bottom of the device, it provides a constant temperature environment, including a metal heat conduction plate, a heating plate, thermal insulation cotton, etc. It is connected to the amplification reaction pool and is used to control the temperature inside the amplification reaction pool.

[0035] Fluorescence Detection Module: Located above the temperature control module, it is responsible for detecting the fluorescence signal in the test tube on the rotating mechanism. The fluorescence detection module includes a light source, a pre-focusing excitation light path, an aperture, a first lens, etc.

[0036] The sensor detects whether a test tube is inserted into the amplification reaction pool. When a test tube is inserted into the amplification reaction pool, the sensor detects the presence of the test tube and sends an electrical signal to the main control module. After receiving the signal, the main control module determines the position of the test tube and triggers the corresponding operation.

[0037] Furthermore, the housing is a prior art component, described in patent CN218491750U. It is the outer protective structure of the entire device and is typically made of durable materials such as plastic or metal. The design of the housing should not only protect the internal components but also facilitate user operation and maintenance.

[0038] The power supply module is responsible for providing the required power for the entire device and is a prior art technology, as described in patent CN218491750U. The power supply module can be a built-in battery, an external power adapter, or a combination of the two.

[0039] The main control module is the central control unit of the device. It is a prior art device described in patent CN218491750U and is generally composed of a microprocessor, memory, and other peripheral circuits. The main control module is responsible for coordinating and controlling all functions of the device.

[0040] The known structure of the above-mentioned prior art will not be described in detail in this application, and the improved structural features are described in detail below.

[0041] See also Figure 1-2 The present invention provides a technical solution: a portable constant-temperature amplification and fluorescence visualization detection device comprising a rotating mechanism 1. The upper surface of the rotating mechanism 1 is provided with a plurality of amplification reaction wells 2 arranged in a ring. The amplification reaction wells 2 are arranged vertically, and test tubes can be inserted into the amplification reaction wells 2 from above and below to secure the test tubes. The surface of the rotating mechanism 1 is marked with Arabic numerals at locations corresponding to the amplification reaction wells 2. To enhance differentiation and reduce errors during hurried operation, each test tube slot is also equipped with a unique color code. Each amplification reaction well 2 is provided with a corresponding sensor. The sensor is connected to a main control module, which is connected to a printing device. Insertion of a test tube triggers the sensor. The sensor signal is transmitted to the main control module, which generates a corresponding barcode or QR code based on the test tube slot number and color code. The barcode / QR code is printed on the test tube cap or pre-attached label using a small printer. Users can apply the label to the test tube while placing the test tube, achieving automatic identification.

[0042] Specifically, based on the original patent CN218491750U, the addition of a rotating mechanism 1 adaptively adjusts the positional relationship between the temperature control module and the fluorescence detection module, namely: the rotating mechanism 1 is located above the fluorescence detection module, and the fluorescence detection module is located above the temperature control module. Through these improvements, the temperature control module can ensure that the test tubes on the rotating mechanism 1 are evenly heated, while the fluorescence detection module can accurately detect the fluorescence signal when the test tubes are rotated to the detection position. This layout not only maintains the compactness of the device, but also improves the accuracy and automation of the detection.

[0043] Specifically, the bottom of the rotating mechanism 1 is provided with a base 3, the upper surface of the base 3 is fixedly connected to a connecting column 4, the rotating mechanism 1 is cylindrical, and the bottom of the rotating mechanism 1 is provided with a connecting groove 5 rotatably connected to the connecting column 4. The device is placed on a table, and the rotating mechanism 1 can rotate relative to the base 3 to facilitate the removal and placement of test tubes;

[0044] Specifically, a first magnet 6 is provided on the lower surface of the rotating mechanism 1. The position of the first magnet 6 corresponds to the amplification reaction pool 2. A second magnet 7 is provided on the upper surface of the base 3 to cooperate with the first magnet 6 to ensure that the test tube is firmly positioned. At the same time, slight positioning feedback is provided during rotation to help the user accurately align each amplification reaction pool 2.

[0045] The rotating mechanism 1 is located above the fluorescence detection module. Its base is equipped with a base 3 and a connecting post 4. The base 3 is rotatably connected to the connecting post 4 via a connecting slot 5, allowing it to rotate relative to the base 3, facilitating the placement and removal of test tubes. A first magnet 6 is located on the lower surface of the rotating mechanism 1, aligned with the amplification reaction pool 2. A second magnet 7, which mates with the first magnet 6, is located on the upper surface of the base 3, ensuring the test tubes are securely positioned and providing gentle positioning feedback to help the user accurately align each amplification reaction pool 2.

[0046] Specifically, a sensor housing 8 is provided on the upper surface of the rotating mechanism 1, and the sensor housing 8 is fixedly connected to the rotating mechanism 1. Figure 2 As shown; through the above design, the rotating mechanism 1 can rotate freely on the base 3, while the magnet setting is used to ensure the stable positioning and positioning feedback of the test tube.

[0047] Since the test tube can be stably placed inside the rotating mechanism 1, it is very convenient to move the test tube, so it plays a portable role. A handle (not shown in the figure) can be provided on the device to further improve the portability.

[0048] Specifically, the interior of the amplification reaction pool 2 is respectively provided with an anti-slip sheet 9, which is made of highly elastic silicone and has good grip to prevent the test tube from shifting during rotation or movement;

[0049] During use, the present invention places a test tube within the amplification reaction cell 2. Rotating the rotating mechanism 1 facilitates the removal and placement of the test tube. Once the test tube is inserted, the sensor within the sensor housing 8 activates, transmitting the sensor signal to the main control module. The main control module generates a corresponding barcode or QR code based on the test tube slot number and color coding. The barcode / QR code is printed on the test tube cap or pre-attached label using a small printer. Users can then attach the label to the test tube while placing the test tube, achieving automatic identification.

[0050] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0051] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A portable constant temperature amplification and fluorescence visualization detection device, comprising a housing, a power supply module, a main control module, a fluorescence detection module, a temperature control module, and an amplification reaction cell, characterized in that: The invention also includes a rotating mechanism (1) arranged above the temperature control module, wherein the upper surface of the rotating mechanism (1) is provided with a plurality of amplification reaction pools (2) distributed in an annular shape, and the upper surface of the rotating mechanism (1) is provided with markings corresponding to the amplification reaction pools (2), and the amplification reaction pools (2) are respectively provided with corresponding sensors, the sensors are wirelessly connected to the main control module, and the main control module is connected to a printing device.

2. The portable constant temperature amplification and fluorescence visualization detection device according to claim 1, characterized in that: The rotating mechanism (1) is located above the fluorescence detection module.

3. The portable constant temperature amplification and fluorescence visualization detection device according to claim 1, characterized in that: The fluorescence detection module is located above the temperature control module.

4. The portable constant temperature amplification and fluorescence visualization detection device according to claim 3, characterized in that: The sensor is connected to the main control module, and when the test tube is inserted into the amplification reaction pool, the sensor sends a signal to the main control module.

5. The portable constant temperature amplification and fluorescence visualization detection device according to claim 1, characterized in that: The side walls of each amplification reaction pool are respectively provided with sensors.

6. The portable constant temperature amplification and fluorescence visualization detection device according to claim 1, characterized in that: The main control module is connected to the printing device. When the test tube is inserted into the amplification reaction pool, the main control module triggers the printing device to print a corresponding label according to the identification of the test tube position.

7. The portable constant temperature amplification and fluorescence visualization detection device according to claim 6, characterized in that: A first magnet (6) is provided on the lower surface of the rotating mechanism (1), and the position of the first magnet (6) corresponds to the amplification reaction pool (2). A base (3) is provided at the bottom of the rotating mechanism (1), and a second magnet (7) that cooperates with the first magnet (6) is provided on the upper surface of the base (3).

8. The portable constant temperature amplification and fluorescence visualization detection device according to claim 7, characterized in that: The interior of the rotating mechanism (1) is in direct contact with the amplification reaction pool (2).

9. The portable constant temperature amplification and fluorescence visualization detection device according to claim 7, characterized in that: Anti-slip sheets (9) are respectively provided inside the amplification reaction pools (2).

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