Reagent card for whole blood separation liquid phase detection

Abstract

The utility model provides a reagent card for whole blood separation liquid phase test, which comprises a card body, a separation cavity and an extraction cavity are arranged on the card body, a liquid outlet is arranged at the bottom of the separation cavity, and the liquid outlet is communicated with the extraction cavity; a filter membrane covering the liquid outlet is fixed in the separation cavity; a supercharging device is arranged in the separation cavity and used for increasing the air pressure in the separation cavity. A whole blood separation structure is arranged on the reagent card, when blood plasma or serum needs to be detected, collected whole blood is added into the separation cavity, the pressurizing device increases the air pressure in the separation cavity, the whole blood permeates the filtering membrane under the dual action of gravity and the air pressure to obtain the blood plasma or serum, the blood plasma or serum flows into the extraction cavity through the liquid outlet, and then the blood plasma or serum is extracted. A centrifugal device or a filtering device does not need to be additionally adopted for treating whole blood, and the operation convenience is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical immunoassay, in particular to a reagent card for whole blood separation liquid phase test. Background Art

[0002] A fully automatic biochemical immunoassay analyzer is a device that performs biochemical and immunoassay tests simultaneously with a single injection. Automated testing is achieved by attaching a reagent card to a holder and then moving the holder to different stations on the analyzer. To accommodate the analyzer, the reagent card is equipped with a sample chamber, a sampling needle holder, a cleaning chamber, a reagent chamber, and a test chamber.

[0003] For example, Chinese patent application number CN208547642U provides a reagent card comprising a card body, the card body being provided with a sample chamber, a sampling needle holder, a cleaning chamber, a reagent chamber, and a detection chamber. The sample chamber is used to hold the sample to be tested, the sampling needle holder is used to hold the sampling needle, the cleaning chamber is used to clean the sampling needle, the reagent chamber is used to store the reagents required for sample testing, and the detection chamber is used to mix and test the sample and reagents. The above-mentioned existing reagent cards do not have a whole blood separation structure. When plasma or serum needs to be tested, the user collects whole blood, separates the whole blood through a centrifuge or filtration device, and then takes the plasma or serum and adds it to the sample chamber of the reagent card, which causes operational inconvenience.

[0004] Therefore, the existing technology has a large room for improvement. Utility Model Content

[0005] The purpose of the utility model is to make up for the deficiencies of the prior art and to provide a reagent card for whole blood separation liquid phase testing.

[0006] In order to achieve the above object, the present invention is implemented through the following technical solutions:

[0007] The utility model provides a reagent card for whole blood separation liquid phase test, comprising a card body, wherein a separation chamber and an extraction chamber are provided on the card body, a liquid outlet is provided at the bottom of the separation chamber, and the liquid outlet is communicated with the extraction chamber; a filter membrane covering the liquid outlet is fixed in the separation chamber; a pressurizing device is provided in the separation chamber, and the pressurizing device is used to increase the air pressure in the separation chamber.

[0008] Through the above-mentioned structural arrangement, the reagent card is provided with a whole blood separation structure. When it is necessary to test plasma or serum, the collected whole blood only needs to be added to the separation chamber. The booster device increases the air pressure in the separation chamber. Under the dual effects of gravity and air pressure, the whole blood permeates the filter membrane to obtain plasma or serum. The plasma or serum flows into the extraction chamber through the liquid outlet. There is no need to use a centrifugal device or a filtering device to process the whole blood, thereby improving the convenience of operation.

[0009] According to the above solution, the pressurizing device includes a sealing plug arranged in the separation chamber. Through the above structural arrangement, the sealing plug is inserted downward into the separation chamber to compress the air in the separation chamber, thereby increasing the air pressure in the separation chamber and improving the filtration efficiency.

[0010] According to the above solution, the pressurizing device further includes a sealing cover fixed to the separation chamber. The filter membrane is disposed on the bottom of the separation chamber, and the sealing cover compresses and secures the filter membrane to the bottom of the separation chamber. The sealing plug is inserted into the inner hole of the sealing cover. The sealing cover secures the filter membrane within the separation chamber.

[0011] It is understandable that in some embodiments, a sealing cover may not be provided, and the filter membrane may be fixed in the separation chamber by other means, such as fixing the filter membrane on the bottom of the separation chamber by hot pressing.

[0012] According to the above solution, the sealing plug is connected to the sealing cover via the anti-lost connector. When whole blood is added to the separation chamber, the sealing plug is first pulled out of the sealing cover; since the anti-lost connector connects the sealing plug to the sealing cover, the sealing plug can be prevented from being lost.

[0013] According to the above solution, the bottom of the extraction chamber is provided with a liquid inlet, the liquid outlet is connected to the liquid inlet, and the liquid outlet is horizontally higher than the liquid inlet. This structural arrangement is conducive to improving the efficiency of plasma or serum flowing from the liquid outlet into the extraction chamber.

[0014] According to the above solution, a siphon channel is provided at the bottom of the card body, a first end of the siphon channel being connected to the liquid outlet, and a second end of the siphon channel being connected to the liquid inlet. The siphon channel has a certain hydrophilic effect, and through the siphon effect, the filtered plasma or serum is quickly guided into the extraction chamber.

[0015] According to the above solution, a siphon groove is formed on the bottom surface of the card body, and a sealing film is fixedly attached to the bottom surface of the card body, and the siphon flow channel is formed between the siphon groove and the sealing film. The siphon flow channel is formed by the cooperation between the siphon groove and the sealing film, thereby improving the processing efficiency of the siphon flow channel.

[0016] According to the above solution, the extraction chamber is configured as a cone with a larger upper portion and a smaller lower portion.

[0017] According to the above scheme, the card body is also provided with a TIP head placement rack, a cleaning chamber, a reagent chamber and a detection chamber. The TIP head is placed in the TIP head placement rack, and the side of the detection chamber is formed as a detection surface; the card body is provided with a heat-sealing film for sealing the cleaning chamber, the reagent chamber and the detection chamber.

[0018] According to the above solution, a buckle portion cooperating with the fixing seat is provided on the side of the card body. The buckle portion cooperates with the fixing seat to fix the card body in the fixing seat.

[0019] The beneficial effects of the present invention are:

[0020] The utility model is provided with a whole blood separation structure on the reagent card. When it is necessary to test the plasma or serum, the collected whole blood only needs to be added into the separation chamber. The booster device increases the air pressure in the separation chamber. Under the dual action of gravity and air pressure, the whole blood permeates the filter membrane to obtain plasma or serum. The plasma or serum flows into the extraction chamber through the liquid outlet. There is no need to use a centrifugal device or a filtering device to process the whole blood, thereby improving the convenience of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural diagram of the utility model;

[0022] Figure 2 It is a structural exploded view of the utility model;

[0023] Figure 3 It is a cross-sectional schematic diagram of the utility model;

[0024] Figure 4 yes Figure 3 Enlarged view of part A;

[0025] Figure 5 It is a partial structural diagram of the utility model;

[0026] Figure 6 It is a structural schematic diagram of the card body of the present invention after the sealing film is removed from the bottom.

[0027] In the figure: 1. Card body; 11. Heat-sealing film; 12. Buckle part; 2. Separation chamber; 21. Liquid outlet; 22. Filter membrane; 3. Extraction chamber; 31. Liquid inlet; 4. Sealing plug; 41. Anti-lost connector; 5. Sealing cover; 6. Siphon flow channel; 61. Siphon groove; 62. Sealing film; 7. TIP head placement rack; 71. TIP head; 8. Cleaning chamber; 9. Reagent chamber; 10. Detection chamber; 101. Detection surface. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0029] like Figure 1-5 As shown, the utility model provides a reagent card for whole blood separation liquid phase test, including a card body 1, a separation chamber 2 and an extraction chamber 3 are provided on the card body 1, a liquid outlet 21 is provided at the bottom of the separation chamber 2, and the liquid outlet 21 is connected to the extraction chamber 3; a filter membrane 22 covering the liquid outlet 21 is fixed in the separation chamber 2; a booster device is provided in the separation chamber 2, and the booster device is used to increase the air pressure in the separation chamber 2.

[0030] Through the above-mentioned structural setting, the reagent card is provided with a whole blood separation structure. When it is necessary to test the plasma or serum, the collected whole blood only needs to be added to the separation chamber 2. The booster device increases the air pressure in the separation chamber 2. Under the dual effects of gravity and air pressure, the whole blood permeates the filter membrane 22 to obtain plasma or serum. The plasma or serum flows into the extraction chamber 3 through the liquid outlet 21. There is no need to use a centrifugal device or a filtering device to process the whole blood, thereby improving the convenience of operation.

[0031] Furthermore, the pressurizing device includes a sealing plug 4 provided in the separation chamber 2. Through the above-mentioned structural arrangement, the sealing plug 4 is inserted downward into the separation chamber 2 to compress the air in the separation chamber 2, thereby increasing the air pressure in the separation chamber 2 and improving the filtration efficiency.

[0032] Furthermore, the pressurizing device further includes a sealing cover 5 fixed to the separation chamber 2. The filter membrane 22 is disposed on the bottom of the separation chamber 2. The sealing cover 5 compresses and secures the filter membrane 22 to the bottom of the separation chamber 2. The sealing plug 4 is inserted into the inner hole of the sealing cover 5. The sealing cover 5 secures the filter membrane 22 within the separation chamber 2.

[0033] Furthermore, the sealing plug 4 is connected to the sealing cover 5 via an anti-lost connector 41. When whole blood is added to the separation chamber 2, the sealing plug 4 is first pulled out from the sealing cover 5; since the anti-lost connector 41 connects the sealing plug 4 to the sealing cover 5, the sealing plug 4 can be prevented from being lost.

[0034] Furthermore, a liquid inlet 31 is provided at the bottom of the extraction chamber 3, the liquid outlet 21 is connected to the liquid inlet 31, and the liquid outlet 21 is horizontally positioned higher than the liquid inlet 31. This structural arrangement is conducive to improving the efficiency of plasma or serum flowing from the liquid outlet 21 into the extraction chamber 3.

[0035] Furthermore, a siphon channel 6 is provided at the bottom of the card body 1, a first end of the siphon channel 6 being connected to the liquid outlet 21, and a second end of the siphon channel 6 being connected to the liquid inlet 31. The siphon channel 6 has a certain hydrophilic effect, and quickly guides the filtered plasma or serum into the extraction chamber 3 through the siphon effect.

[0036] Furthermore, a siphon groove 61 is formed on the bottom surface of the card body 1, and a sealing film 62 is fixedly attached to the bottom surface of the card body 1, and the siphon flow channel 6 is formed between the siphon groove 61 and the sealing film 62. The siphon flow channel 6 is formed by the cooperation between the siphon groove 61 and the sealing film 62, thereby improving the processing efficiency of the siphon flow channel 6.

[0037] Furthermore, the extraction chamber 3 is configured to be in a cone shape with a larger upper portion and a smaller lower portion.

[0038] Furthermore, the card body 1 is also provided with a TIP head placement rack 7, a cleaning chamber 8, a reagent chamber 9 and a detection chamber 10, a TIP head 71 is placed in the TIP head placement rack 7, and the side of the detection chamber 10 is formed as a detection surface 101; the card body 1 is provided with a heat-sealing film 11 for sealing the cleaning chamber 8, the reagent chamber 9 and the detection chamber 10.

[0039] Furthermore, a buckle portion 12 that cooperates with a fixing seat (not shown) is provided on the side of the card body 1. The buckle portion 12 cooperates with the fixing seat, thereby fixing the card body 1 in the fixing seat.

[0040] When using the reagent card for whole blood separation liquid phase test of the present invention, the sealing plug 4 is first pulled out from the sealing cover 5, and then the whole blood is added to the separation chamber 2 from the inner hole of the sealing cover 5, and the sealing plug 4 is inserted downward into the inner hole of the sealing cover 5 to compress the air and increase the air pressure in the separation chamber 2. Under the dual effects of gravity and air pressure, the red blood cells in the whole blood remain on the filter membrane 22, while the plasma or serum is filtered by the filter membrane 22 and enters the extraction chamber 3 in sequence through the liquid outlet 21, the siphon flow channel 6, and the liquid inlet 31, thereby realizing whole blood separation; then the buckle portion 12 on the card body 1 is engaged with the fixing seat to fix the reagent card in the fixing seat, and the heat-sealing film 11 is removed from the card body 1; and then the fixing seat is moved to different stations of the fully automatic biochemical immunoassay to realize automated testing.

[0041] The above description is only a preferred embodiment of the present invention. Therefore, any equivalent changes or modifications made according to the structure, features and principles described in the scope of the present invention patent application are included in the scope of the present invention patent application.

Claims

1. A reagent card for whole blood separation liquid phase test, comprising a card body (1), characterized in that: The card body (1) is provided with a separation chamber (2) and an extraction chamber (3); a liquid outlet (21) is provided at the bottom of the separation chamber (2), and the liquid outlet (21) is communicated with the extraction chamber (3); A filter membrane (22) covering the liquid outlet (21) is fixed in the separation chamber (2); A pressure boosting device is provided in the separation chamber (2), and the pressure boosting device is used to increase the air pressure in the separation chamber (2).

2. The reagent card for whole blood separation liquid phase test according to claim 1, characterized in that: The pressurizing device comprises a sealing plug (4) arranged in the separation chamber (2).

3. The reagent card for whole blood separation liquid phase test according to claim 2, characterized in that: The boosting device further comprises a sealing cover (5) fixed on the separation chamber (2), the filter membrane (22) is arranged on the bottom of the separation chamber (2), and the sealing cover (5) presses and fixes the filter membrane (22) on the bottom of the separation chamber (2); The sealing plug (4) is inserted into the inner hole of the sealing cover (5).

4. The reagent card for whole blood separation liquid phase test according to claim 3, characterized in that: The sealing plug (4) is connected to the sealing cover (5) via an anti-lost connection piece (41).

5. The reagent card for whole blood separation liquid phase test according to claim 1, characterized in that: A liquid inlet (31) is provided at the bottom of the extraction chamber (3), the liquid outlet (21) is in communication with the liquid inlet (31), and the position of the liquid outlet (21) in the horizontal direction is higher than that of the liquid inlet (31).

6. The reagent card for whole blood separation liquid phase test according to claim 5, characterized in that: A siphon flow channel (6) is provided at the bottom of the card body (1); The first end of the siphon flow channel (6) is in communication with the liquid outlet (21), and the second end of the siphon flow channel (6) is in communication with the liquid inlet (31).

7. The reagent card for whole blood separation liquid phase test according to claim 6, characterized in that: A siphon groove (61) is provided on the bottom surface of the card body (1), and a sealing film (62) is fixedly attached to the bottom surface of the card body (1); The siphon flow channel (6) is formed between the siphon groove (61) and the sealing film (62).

8. The reagent card for whole blood separation liquid phase test according to claim 1, characterized in that: The extraction chamber (3) is configured in a cone shape that is larger at the top and smaller at the bottom.

9. The reagent card for whole blood separation liquid phase test according to claim 1, characterized in that: The card body (1) is also provided with a TIP head placement rack (7), a cleaning chamber (8), a reagent chamber (9) and a detection chamber (10); A TIP head (71) is placed in the TIP head placement rack (7), and the side of the detection cavity (10) is formed as a detection surface (101); and a heat-sealing film (11) for sealing the cleaning cavity (8), the reagent cavity (9) and the detection cavity (10) is provided on the card body (1).

10. The reagent card for whole blood separation liquid phase test according to claim 1, characterized in that: A buckle portion (12) that cooperates with the fixing seat is provided on the side of the card body (1).

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