Device for preparing cell suspension from primary tissues

Abstract

The utility model provides a primary tissue cell suspension preparation device which comprises a suspension preparation instrument, a preparation bin is arranged at the inner end of the suspension preparation instrument, a suspension cylinder is arranged at the inner end of the preparation bin, an embedded ring groove is formed in the inner end of the suspension cylinder, a centrifugal counterweight cylinder is arranged at the inner end of the embedded ring groove, and a positioning spring is arranged at the outer end of the centrifugal counterweight cylinder. An embedded groove is formed in the inner end of the suspension cylinder, a positioning shaft is arranged at the inner end of the embedded groove, a positioning rod is arranged at the outer end of the positioning shaft, a hook plate is arranged at the upper end of the positioning rod, an outer ring groove is formed in the outer end of the hook plate, a silica gel ring is arranged at the inner end of the outer ring groove, and a suspension pipe is arranged at the inner end of the suspension cylinder. According to the cell suspension preparation device, the problems that in the design of cell suspension preparation, when a suspension tube is centrifugally rotated, some suspension cylinders are not provided with the suspension tube in a preparation bin, so that the inertia force borne by the vacant suspension cylinders is inconsistent with the inertia force borne by the suspension cylinders containing the suspension tube in the centrifugal movement process, and the centrifugal effect is easily influenced are solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell suspension preparation technical field, specifically, and it is primary tissue preparation cell suspension device. BACKGROUND

[0002] Primary tissue preparation cell suspension device is a kind of equipment for dissociating tissue sample into single cell.Primary tissue preparation cell suspension principle mainly includes two methods of physical mechanical shearing and chemical enzymolysis.Physical mechanical shearing is using homogenizer to scatter tissue or using scissors, copper mesh or tissue grinder to carry out shearing method, mesh rubbing method and grinding method.Chemical enzymolysis is using enzyme, chelating agent (commonly used EDTA) and mechanical method to process animal tissue, to make it dispersed into single cell suspension sample

[0003] The role of primary tissue preparation cell suspension is as follows: obtaining high-quality single cell suspension or subcellular material: this is crucial for subsequent single cell sequencing, primary cell culture, magnetic cell sorting, flow analysis / sorting and other experiments, because the effect of tissue sample dissociation is directly related to the effect of downstream experiment;Reflecting the growth state of cells in vivo: primary cells have shorter time in vitro, and biological characteristics do not change greatly, still retain the genetic characteristics of organism tissue, can better reflect the growth state of cells in vivo, so that more close to physiological function in vivo Data can be obtained, and is more suitable for drug testing, cell differentiation and transformation and other experimental research.

[0004] In the prior art, during the centrifugal rotation of the suspension tube, some suspension cylinders in the preparation bin do not place the suspension tube, which will cause the centrifugal movement process, the inertia force of the empty suspension cylinder and the suspension cylinder with the suspension tube is inconsistent, and the centrifugal effect is easily affected.Therefore, we improve it, and propose primary tissue preparation cell suspension device. UTILITY MODEL CONTENT

[0005] The utility model aims at: in view of the design of present cell suspension preparation, when the suspension tube is centrifugally rotated, some suspension cylinders in the preparation bin do not place the suspension tube, which will cause the centrifugal movement process, the inertia force of the empty suspension cylinder and the suspension cylinder with the suspension tube is inconsistent, and the centrifugal effect is easily affected.

[0006] In order to realize the above-mentioned utility model purposes, the utility model provides the following technical scheme:

[0007] Primary tissue preparation cell suspension device is used to improve the above-mentioned problems.

[0008] The application is as follows:

[0009] The utility model provides a primary tissue preparation cell suspension device, including the suspension preparation appearance, the inner end of suspension preparation appearance is equipped with the preparation bin, the inner end of preparation bin is equipped with the suspension cylinder, the inner end of suspension cylinder is equipped with the inlay ring groove, the inner end of inlay ring groove is equipped with the centrifugal counter weight cylinder, the outer end of centrifugal counter weight cylinder is equipped with the positioning spring, the inner end of suspension cylinder is equipped with the inlay groove, the inner end of inlay groove is equipped with the positioning shaft, the outer end of positioning shaft is equipped with the positioning rod, the upper end of positioning rod is equipped with the hook board, the outer end of hook board is equipped with the outer ring groove, the inner end of outer ring groove is equipped with the silica gel ring, the inner end of suspension cylinder is equipped with the suspension pipe.

[0010] As the preferred technical scheme of the present application, the inner end of the preparation bin is provided with a centrifugal rotating plate, and the upper end of the centrifugal rotating plate is fixedly connected with the suspension cylinder.

[0011] As the preferred technical scheme of the present application, the inlay ring groove is slidingly connected with the centrifugal counter weight cylinder, the lower end of the centrifugal counter weight cylinder is fixedly connected with the upper end of the positioning spring, and the lower end inner surface of the inlay ring groove is fixedly connected with the lower end of the positioning spring.

[0012] As the preferred technical scheme of the present application, the contact surface of the centrifugal rotating plate and the suspension cylinder is through connection, the inlay groove is embeddedly installed on the inner surface of the suspension cylinder, and the positioning shaft is fixedly connected with the inlay groove.

[0013] As the preferred technical scheme of the present application, the outer end of the positioning shaft is movably connected with the positioning rod, the upper end of the positioning rod is fixedly connected with the hook board, and the lower end of the hook board is in contact with the outer surface of the upper end of the centrifugal counter weight cylinder.

[0014] As the preferred technical scheme of the present application, the outer end of the hook board is through connection with the outer ring groove, the outer ring groove is embedded in the inner end of the suspension cylinder, and the outer ring groove is movably connected with the silica gel ring.

[0015] Compared with the prior art, the utility model has the advantages of:

[0016] In the scheme of the present application:

[0017] Through the design of the centrifugal counter weight cylinder and the positioning spring, even if there is a difference in the number of suspension pipes in the suspension cylinder, the device can automatically adjust to ensure the balanced weight distribution during centrifugation, greatly improving the consistency and reliability of the centrifugation effect.

[0018] The combination of the silica gel ring and the outer ring groove not only provides excellent sealing performance, but also ensures the stability of the suspension pipe during high-speed rotation, reduces the risk of sample leakage, and increases the safety of operation.

[0019] The precise positioning mechanism composed of the positioning shaft, the positioning rod and the hook board makes the placement of the suspension pipe more accurate, facilitating the operator to quickly and accurately load and unload the sample, and improving the work efficiency.

[0020] The design of the silica gel ring allows easy adaptation of different sizes of the suspension tube, enhancing the versatility and scope of application of the device, suitable for various experimental needs;

[0021] By precise control of the suspension tube, better centrifugal separation effect is achieved, which is conducive to improving the quality and cell activity of the cell suspension, and thus promoting the accuracy and reliability of the downstream analysis results. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 The overall structure schematic diagram of the primary tissue cell suspension device provided by the present application is shown in the figure;

[0023] Figure 2 The suspension cylinder side sectional structure diagram of the primary tissue cell suspension device provided by the present application is shown in the figure;

[0024] Figure 3 The suspension cylinder side sectional structure diagram of the primary tissue cell suspension device provided by the present application is shown in the figure;

[0025] Figure 4 The suspension cylinder side sectional structure diagram of the primary tissue cell suspension device provided by the present application is shown in the figure; Figure 2 The enlarged structure schematic diagram of the primary tissue cell suspension device provided by the present application is shown in the figure;

[0026] Figure 5 The enlarged structure schematic diagram of the primary tissue cell suspension device provided by the present application is shown in the figure; Figure 3 The enlarged structure schematic diagram of the primary tissue cell suspension device provided by the present application is shown in the figure;

[0027] Figure 6 The cross-sectional structure schematic diagram of the outer ring groove of the primary tissue cell suspension device provided by the present application is shown in the figure.

[0028] Indicated in the figure:

[0029] 1, suspension preparation instrument; 2, preparation bin; 3, suspension tube; 4, suspension cylinder; 5, inner embedded ring groove; 6, centrifugal counterweight cylinder; 7, positioning spring; 8, positioning shaft; 9, positioning rod; 10, hook plate; 11, outer ring groove; 12, silica gel ring. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0031] Therefore, the following detailed description of embodiments of the present application is not intended to limit the scope of the present application as claimed, but merely to indicate some embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application. It should be noted that the embodiments in the present application and the features and technical solutions in the embodiments can be combined with each other without conflict.

[0032] It should be noted that similar reference numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0033] As shown in Figures 1-6 The present embodiment proposes a primary tissue preparation cell suspension device, which comprises a suspension preparation instrument 1, the inner end of the suspension preparation instrument 1 is provided with a preparation bin 2, the inner end of the preparation bin 2 is provided with a suspension cylinder 4, the inner end of the suspension cylinder 4 is provided with an embedded ring groove 5, the inner end of the embedded ring groove 5 is provided with a centrifugal counterweight cylinder 6, the outer end of the centrifugal counterweight cylinder 6 is provided with a positioning spring 7, the inner end of the suspension cylinder 4 is provided with an embedded groove, the inner end of the embedded groove is provided with a positioning shaft 8, the outer end of the positioning shaft 8 is provided with a positioning rod 9, the upper end of the positioning rod 9 is provided with a hook plate 10, the outer end of the hook plate 10 is provided with an outer ring groove 11, the inner end of the outer ring groove 11 is provided with a silica gel ring 12, and the inner end of the suspension cylinder 4 is provided with a suspension pipe 3.

[0034] The suspension preparation instrument 1 is the core part of the whole device, and the preparation bin 2 provided in the inside provides a closed working environment for the preparation of cell suspension, and ensures the sterile condition in the operation process

[0035] The inner end of the preparation bin 2 is provided with a centrifugal rotating plate, the upper end of the centrifugal rotating plate is fixedly connected with the suspension cylinder 4, and the embedded ring groove 5 is embedded and installed at the inner end of the suspension cylinder 4.

[0036] The embedded ring groove 5 is slidably connected with the centrifugal counterweight cylinder 6, the lower end of the centrifugal counterweight cylinder 6 is fixedly connected with the upper end of the positioning spring 7, and the lower end inner surface of the embedded ring groove 5 is fixedly connected with the lower end of the positioning spring 7.

[0037] The contact surface of the centrifugal rotating plate and the suspension cylinder 4 is through connection, the embedded groove is embedded and installed on the inner surface of the suspension cylinder 4, and the positioning shaft 8 is fixedly connected with the embedded groove.

[0038] The outer end of the positioning shaft 8 is movably connected with the positioning rod 9, the upper end of the positioning rod 9 is fixedly connected with the hook plate 10, and the lower end of the hook plate 10 is in contact with the outer surface of the upper end of the centrifugal counterweight cylinder 6.

[0039] The outer end of the hook plate 10 is connected through the outer ring groove 11, and the outer ring groove 11 is embedded in the inner end of the suspension cylinder 4, and the outer ring groove 11 is movably connected with the silica gel ring 12.

[0040] Preparation of the structure of the bin 2:

[0041] Suspension cylinder 4: as the main container for directly bearing the suspension, the inner embedded ring groove 5 is used to accommodate the centrifugal counterweight cylinder 6, which ensures the safety and stability of the suspension tube 3 during the centrifugal process;

[0042] Centrifugal counterweight cylinder 6: by being connected with the positioning spring 7, it can automatically adjust the position with or without the suspension tube 3, maintain the balance state, and avoid the instability of centrifugation caused by uneven weight distribution;

[0043] Positioning spring 7: connected to the bottom of the inner embedded ring groove 5 and the lower end of the centrifugal counterweight cylinder 6, it provides elastic support and adjusts the position change of the centrifugal counterweight cylinder 6 to adapt to the mass balance requirements under different conditions;

[0044] Positioning shaft 8 and positioning rod 9: constitute part of the positioning system, wherein the positioning shaft 8 is fixed in the inner embedded groove, and the positioning rod 9 is movably connected with the positioning shaft 8 to realize the function of moving up and down, further controlling the position of the hook plate 10;

[0045] Hook plate 10: located at the upper end of the positioning rod 9, used to limit the highest position of the centrifugal counterweight cylinder 6 to prevent excessive movement; at the same time, cooperated with the silica gel ring 12 in the outer ring groove 11, it forms the outer protective layer of the suspension tube 3, enhancing the sealing performance;

[0046] Outer ring groove 11 and silica gel ring 12: under the joint action, the silica gel ring 12 can not only tightly cover the suspension tube 3, but also can freely stretch and contract in the outer ring groove 11, adapting to the putting in or taking out of the suspension tube 3, ensuring the sealing while not affecting the operation convenience;

[0047] Centrifugal rotating plate: fixed at the top of the suspension cylinder 4, it is the key component for driving the rotation of the suspension cylinder 4, and the through connection between it and the suspension cylinder 4 ensures the smooth transmission of the rotating action.

[0048] When the suspension tube 3 is placed in the suspension cylinder 4, as the centrifugal rotating plate starts, if the suspension cylinder 4 is not filled with the suspension tube 3, the centrifugal counterweight cylinder 6 is lowered to the lowest point due to gravity, and through the action of the positioning rod 9, the positioning shaft 8 and other components, the cylinder and other suspension cylinders 4 filled with the suspension tube 3 can still maintain the same center of gravity position in the centrifugal state, so as to achieve the dynamic balance of the overall system. This design effectively solves the problem of low centrifugal efficiency and poor effect caused by uneven weight distribution in the traditional method. In particular, the setting of the silica gel ring 12 not only enhances the stability of the suspension tube 3, but also improves the sealing performance of the overall device, which is crucial for biological sample processing and helps to improve the quality and purity of the cell suspension.

[0049] In use, when the suspension tube 3 is centrifuged, some suspension cylinders 4 in the preparation bin 2 may not be placed with the suspension tube 3, which will cause the centrifugal motion process, the inertia forces of the empty suspension cylinder 4 and the suspension cylinder 4 placed with the suspension tube 3 are inconsistent, which will affect the centrifugal effect. In this process, the inner end of the suspension cylinder 4 is connected to the centrifugal counterweight cylinder 6 through the positioning spring 7 and the embedded ring groove 5, and the moving height of the centrifugal counterweight cylinder 6 is limited by the hook plate 10 at the upper end of the centrifugal counterweight cylinder 6. After the suspension tube 3 is placed in the suspension cylinder 4, the centrifugal counterweight cylinder 6 moves downward, driving the positioning rod 9 connected to the hook plate 10 to recover to the center. The power source for the central recovery comes from the silica gel ring 12 wrapped in the outer ring groove 11. Through the setting of the silica gel ring 12, the outer surface of the suspension tube 3 can be stably wrapped, and the surface of the silica gel ring 12 can stably adhere to the surface of the suspension tube 3, ensuring the stability of the centrifugation of the suspension tube 3. In addition, during the centrifugal motion, the empty suspension cylinder 4 is positioned by the upward moving centrifugal counterweight cylinder 6, which can ensure the stability and uniformity of the overall centrifugation.

[0050] The above embodiments are only used to illustrate the technical solutions described in the present application and are not limited to the technical solutions described in the present application. Although the present application has been described in detail with reference to the above embodiments, the present application is not limited to the above specific embodiments. Therefore, any modification or equivalent replacement of the present application; and all technical solutions and improvements within the spirit and scope of the present application are covered by the claims of the present application.

Claims

1. A device for preparing a cell suspension from a primary tissue, comprising a suspension preparation instrument (1), characterized in that, The inner end of the suspension preparation instrument (1) is provided with a preparation bin (2), the inner end of the preparation bin (2) is provided with a suspension cylinder (4), the inner end of the suspension cylinder (4) is provided with an embedded ring groove (5), the inner end of the embedded ring groove (5) is provided with a centrifugal counterweight cylinder (6), the outer end of the centrifugal counterweight cylinder (6) is provided with a positioning spring (7), the inner end of the suspension cylinder (4) is provided with an embedded groove, the inner end of the embedded groove is provided with a positioning shaft (8), the outer end of the positioning shaft (8) is provided with a positioning rod (9), the upper end of the positioning rod (9) is provided with a hook plate (10), the outer end of the hook plate (10) is provided with an outer ring groove (11), the inner end of the outer ring groove (11) is provided with a silica gel ring (12), and the inner end of the suspension cylinder (4) is provided with a suspension pipe (3).

2. The primary tissue preparation cell suspension device of claim 1, wherein, The inner end of the preparation bin (2) is provided with a centrifugal rotating plate, and the upper end of the centrifugal rotating plate is fixedly connected with the suspension cylinder (4).

3. The primary tissue preparation cell suspension device of claim 2, wherein, The embedded ring groove (5) is slidably connected with the centrifugal counterweight cylinder (6), the lower end of the centrifugal counterweight cylinder (6) is fixedly connected with the upper end of the positioning spring (7), and the lower end inner surface of the embedded ring groove (5) is fixedly connected with the lower end of the positioning spring (7).

4. The primary tissue preparation cell suspension device of claim 3, wherein, The contact surface of the centrifugal rotating plate and the suspension cylinder (4) is through connection, the embedded groove is embeddedly installed on the inner surface of the suspension cylinder (4), and the positioning shaft (8) is fixedly connected with the embedded groove.

5. The primary tissue preparation cell suspension device of claim 4, wherein, The outer end of the positioning shaft (8) is movably connected with the positioning rod (9), the upper end of the positioning rod (9) is fixedly connected with the hook plate (10), and the lower end of the hook plate (10) is in contact with the outer surface of the upper end of the centrifugal counterweight cylinder (6).

6. The primary tissue preparation cell suspension device of claim 5, wherein, The outer end of the hook plate (10) is throughly connected with the outer ring groove (11), the outer ring groove (11) is embedded in the inner end of the suspension cylinder (4), and the outer ring groove (11) is movably connected with the silica gel ring (12).

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