Multifunctional centrifugal tube convenient for cell separation and purification

Abstract

The utility model belongs to the field of centrifuge tubes, and particularly relates to a multifunctional centrifuge tube convenient for cell separation and purification, which comprises a tube body and a separation and purification net, the outer side of an opening at the upper end of the tube body is connected with a tube cover through threads, the separation and purification net is positioned on the inner side of the tube body, and the top of the separation and purification net is fixedly connected with a backing ring. A backing ring is arranged in the pipe body and makes contact with the top of the pipe body, a flange is fixedly connected to the outer wall of the pipe body, a clamping pin is slidably connected to the interior of the pipe cover, the clamping pin is connected with the flange in a clamped mode, and a spring is arranged in the pipe cover. The separating and purifying net with the backing ring is arranged in the tube body and used for separating and purifying cells, the functionality of the centrifugal tube is improved, meanwhile, the flange is arranged on the outer side of the tube body, the clamping pin stretching out and drawing back through the spring is arranged in the tube cover, and the clamping pin can be clamped into the flange under the action of the spring, so that the cell separation and purification effect is improved. Therefore, the tube cover has a certain anti-loosening effect, so that the tube cover is not easy to loosen during centrifugation.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifugal tube technical field, concretely to the multifunctional centrifugal tube convenient for cell separation and purification. BACKGROUND

[0002] The centrifugal tube with the cell filter filters the cell solution in advance when pouring into the centrifugal tube, and the large volume cells and impurities are filtered out, so as to facilitate the separation and purification of cells.

[0003] According to the search, in the utility model patent with patent grant announcement No. CN206052013U, a centrifugal tube with a cell filter is disclosed, which comprises a pipe body with an upper opening, a filter cartridge is arranged at the upper end of the pipe body, a baffle is arranged at the upper end of the filter cartridge for fixing the filter cartridge on the pipe body, a bevel is arranged on the filter cartridge, a sharp end part is formed by the lower end of the filter cartridge and the bevel, and a first filter membrane is arranged on the filter cartridge to cover the bevel.

[0004] However, the pipe cover of the centrifugal tube is installed by screwing, and it is easy to loosen under the action of centrifugal force, which leads to cell leakage. In addition, the cover of the centrifugal tube is usually sealed, and it usually does not have air permeability to prevent leakage. UTILITY MODEL CONTENT

[0005] The utility model discloses a multifunctional centrifugal tube convenient for cell separation and purification, which solves the problem of loosening of the pipe cover of the existing centrifugal tube under the action of centrifugal force due to the installation by screwing, and solves the problem of air permeability of the cover of the centrifugal tube.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a multifunctional centrifugal tube convenient for cell separation and purification, comprising a pipe body and a separation and purification net, the upper end of the pipe body is connected with a pipe cover through threads on the outside, the separation and purification net is located on the inside of the pipe body, the top of the separation and purification net is fixedly connected with a gasket ring, the gasket ring is in contact with the top of the pipe body, the outer wall of the pipe body is fixedly connected with a flange, the inside of the pipe cover is slidably connected with a pin, the pin is connected with the flange, and the inside of the pipe cover is provided with a spring.

[0007] Preferably, one end of the spring is fixedly connected with the pin, and the other end of the spring is fixedly connected with the inner surface of the pipe cover. Through the setting of the spring, the elastic force can be applied to the pin.

[0008] Preferably, the inner wall of the pipe cover is fixedly connected with a stop ring, and the stop ring is in contact with the gasket ring. Through the setting of the stop ring, the separation and purification net and the gasket ring are limited.

[0009] Preferably, the inside of the pipe cover is fixedly connected with a hollow shell, and the hollow shell is arranged through the pipe cover, the hollow shell is slidingly connected to the inside of the gasket ring, a through hole is formed in the bottom of the hollow shell, an adjusting rod is rotatably connected to the top of the hollow shell and arranged through the hollow shell, a adjusting sleeve is threadedly connected to the lower segment of the rod body of the adjusting rod, a baffle is fixedly connected to the bottom of the adjusting sleeve and in contact with the inside bottom of the hollow shell, and an exhaust hole is formed in the top of the hollow shell. The threaded movement of the adjusting rod and the adjusting sleeve can control the lifting of the baffle, so that the through hole in the bottom of the hollow shell is exposed.

[0010] Preferably, a knob is fixedly connected to the top of the adjusting rod and slidingly connected with the hollow shell. The knob is arranged to facilitate the rotation of the adjusting rod.

[0011] Preferably, a limiting ring is fixedly connected to the rod body of the adjusting rod and in contact with the inner wall of the hollow shell. The limiting ring is arranged to limit the adjusting rod.

[0012] Preferably, a guide sleeve is fixedly connected to the baffle, a guide frame is slidingly connected to the inside of the guide sleeve, and the tail end of the guide frame is fixedly connected to the inner wall of the hollow shell. The guide sleeve and the guide frame are arranged to guide the lifting of the baffle.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] 1. The utility model discloses a separating and purifying net with a gasket ring arranged in the pipe body, which is used for the separation and purification of cells, improves the functionality of the centrifugal tube, and the flange is arranged on the outside of the pipe body, and the clamping pin arranged in the pipe cover is telescoped by the spring, the clamping pin is clamped into the flange under the action of the spring, which has the anti-loosening effect on the pipe cover, and the pipe cover is not easy to loosen during centrifugation.

[0015] 2. The utility model discloses a hollow shell arranged in the middle of the pipe cover, exhaust holes and through holes are formed in the top and bottom of the hollow shell respectively, and the baffle is arranged in the hollow shell and adjusted by the adjusting rod and the adjusting sleeve, so that the through hole is exposed when the baffle is adjusted to rise, and the purpose of exhausting the pipe body is achieved. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the overall structure perspective drawing of the utility model;

[0017] Figure 2 It is the partial structure schematic view of the utility model; Figure 1

[0018] Figure 3 It is the front view sectional view of the utility model; Figure 1 ​​

[0019] Figure 4 For the partial structure of the utility model Figure 3 enlarged view of A place of the utility model

[0020] Figure 5 For the partial structure of the utility model Figure 4 enlarged view of A place of the utility model

[0021] In the drawing: 1, pipe body;2, pipe cover;3, separation and purification net;4, gasket ring;5, baffle ring;6, flange;7, bayonet catch;8, spring;9, hollow shell;10, through hole;11, adjusting rod;12, adjusting sleeve;13, baffle;14, knob;15, limit ring;16, guide frame;17, guide sleeve;18, exhaust hole. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0023] Please refer to Figures 1-5 The utility model discloses a multifunctional centrifugal tube convenient for cell separation and purification, which comprises a pipe body 1 and a separation and purification net 3. The upper end of the pipe body 1 is provided with a pipe cover 2 through threaded connection on the outside. The separation and purification net 3 is located on the inside of the pipe body 1. The top of the separation and purification net 3 is fixedly connected with a gasket ring 4, which is in contact with the top of the pipe body 1. The outer wall of the pipe body 1 is fixedly connected with a flange 6. The inside of the pipe cover 2 is slidably connected with a bayonet catch 7, which is clamped with the flange 6. The inside of the pipe cover 2 is provided with a spring 8. One end of the spring 8 is fixedly connected with the bayonet catch 7, and the other end is fixedly connected with the inner surface of the pipe cover 2. The spring 8 can exert elastic force on the bayonet catch 7. The inner wall of the pipe cover 2 is fixedly connected with a baffle ring 5, which is in contact with the gasket ring 4. The baffle ring 5 can limit the separation and purification net 3 and the gasket ring 4.

[0024] Please refer to Figures 1-4The inner part of the pipe cover 2 is fixedly connected with a hollow shell 9, the hollow shell 9 is arranged through the pipe cover 2, the hollow shell 9 is slidingly connected to the inner side of the gasket ring 4, a through hole 10 is formed in the bottom of the hollow shell 9, an adjusting rod 11 is rotatably connected to the top of the hollow shell 9 and arranged through the hollow shell 9, an adjusting sleeve 12 is threadedly connected to the lower segment of the rod body of the adjusting rod 11, a baffle 13 is fixedly connected to the bottom of the adjusting sleeve 12 and in contact with the inner bottom of the hollow shell 9, and an exhaust hole 18 is formed in the top of the hollow shell 9. The threaded movement of the adjusting rod 11 and the adjusting sleeve 12 can control the lifting of the baffle 13, so that the through hole 10 in the bottom of the hollow shell 9 is exposed. A knob 14 is fixedly connected to the top of the adjusting rod 11 and slidingly connected with the hollow shell 9. The setting of the knob 14 facilitates the rotation of the adjusting rod 11. A limiting ring 15 is fixedly connected to the rod body of the adjusting rod 11 and in contact with the inner wall of the hollow shell 9. The limiting ring 15 has a limiting effect on the adjusting rod 11. A guide sleeve 17 is fixedly connected to the baffle 13, a guide frame 16 is slidingly connected to the inner side of the guide sleeve 17, and the distal end of the guide frame 16 is fixedly connected to the inner wall of the hollow shell 9. The guide sleeve 17 and the guide frame 16 have a lifting guide effect on the baffle 13.

[0025] The specific implementation process of the utility model is as follows: in use, first, the separation and purification net 3 is placed into the pipe body 1 and supported by the gasket ring 4, then the cell liquid is poured into it, the cells are separated in size under the filtration of the separation and purification net 3 and impurities are filtered, then the pipe cover 2 is screwed on the pipe body 1 until the retaining ring 5 abuts against the gasket ring 4, the catch pin 7 is clamped into the flange 6 of the pipe body 1 under the action of the spring 8, then it can be placed into a centrifuge for centrifugation, after centrifugation, the adjusting rod 11 is rotated by the knob 14, the adjusting sleeve 12 moves in the axial direction, and the baffle 13 is lifted, then the baffle 13 exposes the through hole 10, so that the gas in the pipe body 1 can be discharged through the through hole 10 and the exhaust hole 18.

[0026] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A multifunctional centrifuge tube for easy cell separation and purification, comprising a tube body (1) and a separation and purification mesh (3), characterized in that: The upper opening of the tube body (1) is connected to a tube cap (2) by a thread. The separation and purification mesh (3) is located inside the tube body (1). A gasket (4) is fixedly connected to the top of the separation and purification mesh (3), and the gasket (4) is in contact with the top of the tube body (1). A flange (6) is fixedly connected to the outer wall of the tube body (1). A locking pin (7) is slidably connected inside the tube cap (2). The locking pin (7) is engaged with the flange (6). A spring (8) is provided inside the tube cap (2).

2. The multifunctional centrifuge tube for easy cell separation and purification according to claim 1, characterized in that: One end of the spring (8) is fixedly connected to the locking pin (7), and the other end of the spring (8) is fixedly connected to the inner surface of the tube cap (2).

3. The multifunctional centrifuge tube for easy cell separation and purification according to claim 1, characterized in that: A retaining ring (5) is fixedly connected to the inner wall of the pipe cap (2), and the retaining ring (5) abuts against the gasket (4).

4. The multifunctional centrifuge tube for easy cell separation and purification according to claim 1, characterized in that: A hollow shell (9) is fixedly connected inside the tube cap (2), and the hollow shell (9) is set through the tube cap (2). The hollow shell (9) is slidably connected to the inner side of the gasket (4). A through hole (10) is opened at the bottom of the hollow shell (9). An adjusting rod (11) is rotatably connected to the top of the hollow shell (9), and the adjusting rod (11) is set through the hollow shell (9). An adjusting sleeve (12) is threadedly connected to the lower section of the adjusting rod (11). A baffle (13) is fixedly connected to the bottom of the adjusting sleeve (12), and the baffle (13) contacts the inner bottom of the hollow shell (9). An exhaust hole (18) is opened at the top of the hollow shell (9).

5. The multifunctional centrifuge tube for easy cell separation and purification according to claim 4, characterized in that: A knob (14) is fixedly connected to the top of the adjusting rod (11), and the knob (14) is slidably connected to the hollow shell (9).

6. The multifunctional centrifuge tube for easy cell separation and purification according to claim 4, characterized in that: A limiting ring (15) is fixedly connected to the rod body of the adjusting rod (11), and the limiting ring (15) is in contact with the inner wall of the hollow shell (9).

7. The multifunctional centrifuge tube for easy cell separation and purification according to claim 4, characterized in that: A guide sleeve (17) is fixedly connected to the baffle (13), and a guide frame (16) is slidably connected to the inner side of the guide sleeve (17), and the end of the guide frame (16) is fixedly connected to the inner wall of the hollow shell (9).

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