Repairing agent for skin defect and preparation method thereof

Abstract

The invention discloses a repairing agent for a skin defect and a preparation method thereof. Each milliliter liquid of the repairing agent for the skin defect contains 10-50 million cells; the cells are dermal fibroblastic stem cells, fibroblastic precursor cells and fibroblasts which are collected after the cut neonatal preputial skin is subjected to in-vitro low serum primary culture and passage amplification; and the liquid is prepared by mixing amino acid injection with 5% of glucose injection at a volume ratio of 1:0.5-2. The repairing agent provided by the invention has the following advantages that the repairing agent for the skin defect has more cell viability and amplification; the combined action without rejection is achieved on the external applied parts and skin tissues; the obvious filling and repairing effects are achieved after the repairing agent is externally applied for one week; and the curative effect is lasting and the treatment schedule is simple and convenient.

Description

technical field

[0001] The invention relates to a tissue filler and a preparation method thereof, more specifically, to a repairing agent for skin defects and a preparation method thereof. Background technique

[0002] Human soft tissue augmentation is the main treatment method for repairing soft tissue after injury. In the nineteenth century, paraffin and silicone were used for filling. After the 1980s, gelatin powder materials appeared again, but they all had problems such as uncertain curative effect and large toxic and side effects.

[0003] After 1997, Isolagen used autologous fibroblasts as tissue filling materials, which had a certain curative effect, but there were problems such as slow and insignificant curative effect.

[0004] Chinese patent 03155833.X discloses an injection for removing wrinkles and scars. It uses autologous skin, which is isolated, cultured and amplified in vitro, and is made into an injection in glucose solution to treat scars and remove wrin...

Examples

Embodiment 1

[0025] A repairing agent for skin defects, the repairing agent contains 30 million cells per milliliter of liquid; the cells are collected from the excised foreskin skin of newborns after in vitro low-serum primary culture and passage expansion dermal fibroblast stem cells, fibroblast precursor cells and fibroblasts, the liquid is prepared by mixing amino acid injection and 5% glucose injection at a volume ratio of 1:1.

Embodiment 2

[0027] A repairing agent for skin defects, the repairing agent contains 20 million cells per milliliter of liquid; the cells are collected after in vitro low-serum primary culture and passage expansion with the circumcised skin of newborns dermal fibroblast stem cells and fibroblast precursor cells, the liquid is prepared by mixing amino acid injection and 5% glucose injection with a volume ratio of 1:0.5.

Embodiment 3

[0029] A repairing agent for skin defects, the repairing agent contains 10 million cells per milliliter of liquid; the cells are collected after in vitro low-serum primary culture and passage expansion with the circumcised skin of newborns dermal fibroblast stem cells and fibroblasts, the liquid is prepared by mixing amino acid injection with a volume ratio of 1:2 and 5% glucose injection.

technical field

[0001] The invention relates to a tissue filler and a preparation method thereof, more specifically, to a repairing agent for skin defects and a preparation method thereof. Background technique

[0002] Human soft tissue augmentation is the main treatment method for repairing soft tissue after injury. In the nineteenth century, paraffin and silicone were used for filling. After the 1980s, gelatin powder materials appeared again, but they all had problems such as uncertain curative effect and large toxic and side effects.

[0003] After 1997, Isolagen used autologous fibroblasts as tissue filling materials, which had a certain curative effect, but there were problems such as slow and insignificant curative effect.

[0004] Chinese patent 03155833.X discloses an injection for removing wrinkles and scars. It uses autologous skin, which is isolated, cultured and amplified in vitro, and is made into an injection in glucose solution to treat scars and remove wrin...

Examples

Embodiment 1

[0025] A repairing agent for skin defects, the repairing agent contains 30 million cells per milliliter of liquid; the cells are collected from the excised foreskin skin of newborns after in vitro low-serum primary culture and passage expansion dermal fibroblast stem cells, fibroblast precursor cells and fibroblasts, the liquid is prepared by mixing amino acid injection and 5% glucose injection at a volume ratio of 1:1.

Embodiment 2

[0027] A repairing agent for skin defects, the repairing agent contains 20 million cells per milliliter of liquid; the cells are collected after in vitro low-serum primary culture and passage expansion with the circumcised skin of newborns dermal fibroblast stem cells and fibroblast precursor cells, the liquid is prepared by mixing amino acid injection and 5% glucose injection with a volume ratio of 1:0.5.

Embodiment 3

[0029] A repairing agent for skin defects, the repairing agent contains 10 million cells per milliliter of liquid; the cells are collected after in vitro low-serum primary culture and passage expansion with the circumcised skin of newborns dermal fibroblast stem cells and fibroblasts, the liquid is prepared by mixing amino acid injection with a volume ratio of 1:2 and 5% glucose injection.

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