Method for increasing capsorubin content in capsicums

Abstract

The invention relates to a method used for increasing capsorubin content in capsicums. Breeding methods of plant new varieties are plenty, and mainly comprise natural variation, crossbreeding, space breeding and ion beam implantation mutation breeding, but the frequency of the natural mutation is low. The method of the invention comprises following steps: burying capsicum seeds vertically in the flower mud of a culture dish at equal intervals with germs upwards and exposed; placing the prepared capsicum seed culture dish in an ion beam implantation vacuum system with the culture dish facing an ion source; reducing the pressure of the vacuum system to 10 to 4 Pa; turning on the ion radiation source, adjusting technological parameters of ion radiation; and performing ion radiation, turning off the ion radiation when ion radiation is complete, inflating the vaccum system, and taking the capsicum seeds out of the vaccum system for culturing. Beneficial effects of the ion beam implantation mutation breeding method of the invention are that: the technological parameters, ion energy and implantation dosage are convenient to adjust and control, adaptability is high, variation frequency is high, variation spectrum is wide, and stabilization is high.

Description

technical field

[0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for increasing the content of capsanthin. Background technique

[0002] Pepper is an important vegetable crop. In addition to being used as a condiment, it also has high nutritional value. It is rich in carbohydrates, protein, three vitamins, calcium, phosphorus, and iron minerals needed by the human body, and is needed by the human body. 15 amino acids. With the improvement of people's living standards, people are very concerned about the capsaicin and red pigment of capsicum, that is, the taste and ornamental properties of capsicum. At present, there are a large number of documents mentioning how to improve the capsicum extracted from capsicum by various methods, but little attention is paid to how to increase the capsicum content of capsicum itself, that is, to increase the content of capsicum in capsicum when cultivating new varieties of cap...

Examples

Embodiment 1

[0030] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0031] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0032] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0033] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 45kev, and the implantation dose is 1×10 15 ions / mm 2 ;

[0034] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0035] Peppers were bred by ...

Embodiment 2

[0037] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0038] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0039] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0040] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 30kev, and the implantation dose is 2×10 16 ions / mm 2 ;

[0041] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0042] Breeding of capsicum b...

Embodiment 3

[0044] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0045] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0046] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0047] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 15kev, and the implantation dose is 4×10 16 ions / mm 2 ;

[0048] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0049] The capsicum was bred ...

technical field

[0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for increasing the content of capsanthin. Background technique

[0002] Pepper is an important vegetable crop. In addition to being used as a condiment, it also has high nutritional value. It is rich in carbohydrates, protein, three vitamins, calcium, phosphorus, and iron minerals needed by the human body, and is needed by the human body. 15 amino acids. With the improvement of people's living standards, people are very concerned about the capsaicin and red pigment of capsicum, that is, the taste and ornamental properties of capsicum. At present, there are a large number of documents mentioning how to improve the capsicum extracted from capsicum by various methods, but little attention is paid to how to increase the capsicum content of capsicum itself, that is, to increase the content of capsicum in capsicum when cultivating new varieties of cap...

Examples

Embodiment 1

[0030] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0031] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0032] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0033] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 45kev, and the implantation dose is 1×10 15 ions / mm 2 ;

[0034] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0035] Peppers were bred by ...

Embodiment 2

[0037] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0038] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0039] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0040] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 30kev, and the implantation dose is 2×10 16 ions / mm 2 ;

[0041] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0042] Breeding of capsicum b...

Embodiment 3

[0044] Step 1: Insert the pepper seeds vertically and evenly into the flower mud of the petri dish, with the germ facing up, exposing the germ part of the pepper seeds. In order to ensure the probability of mutation breeding, there should be at least 500 pepper seeds;

[0045] Step 2: Place the prepared pepper seed Petri dish sample in the ion implantation vacuum system, facing the ion source, and select the gas ion source or metal ion source as the radiation source.

[0046] Step 3: Vacuum the vacuum system to 10 -4 Pa;

[0047] Step 4: Turn on the ion radiation source, adjust the ion radiation process parameters, the ion radiation process parameters include ion energy and implantation dose, the ion energy is 15kev, and the implantation dose is 4×10 16 ions / mm 2 ;

[0048] Step five: carry out ion radiation, turn off the ion radiation source after completion, inflate the vacuum system, take out the dry seeds of pepper seeds for cultivation.

[0049] The capsicum was bred ...