A culture medium for improving the survival rate of boletus mycelium isolation

Abstract

The invention discloses a culture medium for improving the separation survival rate of boletus mycelium. The culture medium is prepared from the following raw materials in proportions: dried boletus powder, a potato cooking liquid, dipotassium hydrogen phosphate, magnesium sulfate, glucose, potassium dihydrogen phosphate and agar. The culture medium disclosed by the invention has the beneficial effects that under the action of the dried boletus powder, the infection of other fungi can be reduced, and the separation survival rate of boletus mycelium can be effectively improved; the culture medium is convenient and fast to select materials; the changed culture medium is stronger in pertinence, reasonable in formula and balanced in nutrition, and can be used for fully meeting the nutrition requirements for growth of boletus mycelium; and cultured mycelium is healthy and stronger in vitality, can be used for effectively reducing the infection of other fungi and effectively improving the separation survival rate of boletus mycelium, and is significant in use effect.

Description

technical field

[0001] The invention relates to a culture medium, in particular to a culture medium for improving the isolated survival rate of boletus mycelium. Background technique

[0002] Boletus, also known as Sagittarius, is a precious edible fungus in the world. It has high edible value and medicinal value. However, boletus belongs to mycorrhizal fungi, and most varieties cannot be artificially cultivated. Wild resources of boletus The lack of it hinders further research.

[0003] The mycelium of boletus has been favored by many scholars as a research material, but boletus is a symbiotic fungus. At present, there are few researches on boletus in China, and the symbiotic plant types of many boletus species cannot be determined. , thus bringing great difficulty to the survival rate of boletus mycelium separation.

[0004] At present, the culture medium for the isolation of boletus mainly adopts the general medium for the separation of conventional edible fungi or adds...

Examples

Embodiment 1

[0017] This medium for improving the survival rate of boletus mycelium separation is made of the following raw material proportions in parts by weight: 5 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0018] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0019] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 30 minutes, and after pulverization, a 200-mesh sieve is passed to obtain a dried boletus powder;

[0020] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, chops, boils ...

Embodiment 2

[0025] This culture medium for improving the separation and survival rate of boletus mycelium is made of the following raw material proportions in parts by weight: 7 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0026] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0027] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 35 minutes, and after pulverizing, pass through a 200-mesh sieve to obtain dried boletus powder;

[0028] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, ch...

Embodiment 3

[0033] This culture medium for improving the isolation and survival rate of boletus mycelium is made of the following raw material ratios in parts by weight: 8 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0034] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0035] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 40 minutes, and after pulverizing, pass through a 200-mesh sieve to obtain dried boletus powder;

[0036] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, chops, b...

technical field

[0001] The invention relates to a culture medium, in particular to a culture medium for improving the isolated survival rate of boletus mycelium. Background technique

[0002] Boletus, also known as Sagittarius, is a precious edible fungus in the world. It has high edible value and medicinal value. However, boletus belongs to mycorrhizal fungi, and most varieties cannot be artificially cultivated. Wild resources of boletus The lack of it hinders further research.

[0003] The mycelium of boletus has been favored by many scholars as a research material, but boletus is a symbiotic fungus. At present, there are few researches on boletus in China, and the symbiotic plant types of many boletus species cannot be determined. , thus bringing great difficulty to the survival rate of boletus mycelium separation.

[0004] At present, the culture medium for the isolation of boletus mainly adopts the general medium for the separation of conventional edible fungi or adds...

Examples

Embodiment 1

[0017] This medium for improving the survival rate of boletus mycelium separation is made of the following raw material proportions in parts by weight: 5 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0018] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0019] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 30 minutes, and after pulverization, a 200-mesh sieve is passed to obtain a dried boletus powder;

[0020] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, chops, boils ...

Embodiment 2

[0025] This culture medium for improving the separation and survival rate of boletus mycelium is made of the following raw material proportions in parts by weight: 7 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0026] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0027] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 35 minutes, and after pulverizing, pass through a 200-mesh sieve to obtain dried boletus powder;

[0028] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, ch...

Embodiment 3

[0033] This culture medium for improving the isolation and survival rate of boletus mycelium is made of the following raw material ratios in parts by weight: 8 grams of dried boletus powder, 200 grams of potato cooking liquid, and 0.5 grams of dipotassium hydrogen phosphate. gram, 1.5 grams of magnesium sulfate, 20 grams of glucose, 1 gram of potassium dihydrogen phosphate, and 15 grams of agar.

[0034] The preparation method of the culture medium of above-mentioned improving boletus mycelium separation survival rate, it is carried out by following steps:

[0035] The first step, the preparation of dried boletus powder, the dried boletus to be separated is pulverized by an ultrafine pulverizing vibrating mill, and the pulverization time is 40 minutes, and after pulverizing, pass through a 200-mesh sieve to obtain dried boletus powder;

[0036] The second step, the processing of potato boiled liquid, selects the potato that does not turn green, does not sprout, peels, chops, b...