Method for detecting four tobacco-specific nitrosamines (TSNAs) in lateral exhaust gas of cigarettes by virtue of gas chromatography-tandem mass spectrometry

Abstract

The invention relates to a method for detecting four tobacco-specific nitrosamines (TSNAs) in lateral exhaust gas of cigarettes by virtue of gas chromatography-tandem mass spectrometry. The method is characterized by comprising the steps of capturing the lateral exhaust gas of the cigarettes by virtue of a lateral fishtail cover preprocessed by an ascorbic acid solution and a Cambridge filter preprocessed by an ascorbic acid saturated methanol solution, washing the lateral fishtail cover with a hydrochloric acid solution, adding the washed hydrochloric acid solution and an TSNAs internal standard solution into a container in which the Cambridge filter is collected, extracting the Cambridge filter, processing extraction solution by virtue of a solid phase extraction column to obtain an eluate, drying the eluate with nitrogen, redissolving by virtue of dichloromethane to obtain a sample, and analyzing the sample by virtue of the gas chromatography-tandem mass spectrometry (GC-MS / MS). According to the method, the disadvantages of tedious preprocessing process, low detection sensitivity and strong matrix effect in an existing method are overcome, the preprocessing step of the sample is simplified, and the consumption of organic solvents is reduced; the method has the advantages that the detection is accurate, the sensitivity is high, and the repeatability is good.

Description

technical field

[0001] The invention belongs to the technical field of cigarette smoke analysis and detection, and in particular relates to a gas chromatography-tandem mass spectrometry (GC-MS / MS) method for detecting four tobacco-specific nitrosamines (TSNAs) in cigarette sidestream smoke. Background technique

[0002] At present, the governments, health organizations and people of various countries are paying more and more attention to smoking and health issues, and the harmful components in sidestream smoke of cigarettes have also become one of the focuses of people's attention. Cigarette sidestream smoke is composed of smoke produced when a cigarette smolders between puffs and smoke that diffuses through the paper during puffing. It is one of the important sources of environmental tobacco smoke (ETS), accounting for about 85% of the total ETS. Some epidemiological studies have shown that exposure to ETS increases the risk of respiratory, cardiovascular and lung canc...

Examples

Embodiment Construction

[0026] The present invention will be further described below in conjunction with embodiment:

[0027] According to the method stipulated in GB / T28971-2012, use ascorbic acid solution to pretreat the side stream fish tail cover, and use ascorbic acid saturated methanol solution to pretreat the Cambridge filter.

[0028] Balance and extract the cigarette samples to be tested, smoke them on a sidestream smoking machine according to the standard conditions specified in YC / T 185, and smoke 2 cigarettes for each experiment. Use 40 mL of pH=1 hydrochloric acid solution to wash the side-flow fishtail cover twice, and transfer the washed hydrochloric acid solution to a 50 mL ground-mouth Erlenmeyer flask with a Cambridge filter, add 80 μL of d 4 -NNN,d 4 -NAT, d 4 -NAB,d 4 -Methanol solution with NNK concentration of 1 μg / mL was used as the internal standard of TSNAs. Ultrasonic extraction was performed on the Cambridge filter, and the extraction time was 30 min.

[0029] The extr...

technical field

[0001] The invention belongs to the technical field of cigarette smoke analysis and detection, and in particular relates to a gas chromatography-tandem mass spectrometry (GC-MS / MS) method for detecting four tobacco-specific nitrosamines (TSNAs) in cigarette sidestream smoke. Background technique

[0002] At present, the governments, health organizations and people of various countries are paying more and more attention to smoking and health issues, and the harmful components in sidestream smoke of cigarettes have also become one of the focuses of people's attention. Cigarette sidestream smoke is composed of smoke produced when a cigarette smolders between puffs and smoke that diffuses through the paper during puffing. It is one of the important sources of environmental tobacco smoke (ETS), accounting for about 85% of the total ETS. Some epidemiological studies have shown that exposure to ETS increases the risk of respiratory, cardiovascular and lung canc...

Examples

Embodiment Construction

[0026] The present invention will be further described below in conjunction with embodiment:

[0027] According to the method stipulated in GB / T28971-2012, use ascorbic acid solution to pretreat the side stream fish tail cover, and use ascorbic acid saturated methanol solution to pretreat the Cambridge filter.

[0028] Balance and extract the cigarette samples to be tested, smoke them on a sidestream smoking machine according to the standard conditions specified in YC / T 185, and smoke 2 cigarettes for each experiment. Use 40 mL of pH=1 hydrochloric acid solution to wash the side-flow fishtail cover twice, and transfer the washed hydrochloric acid solution to a 50 mL ground-mouth Erlenmeyer flask with a Cambridge filter, add 80 μL of d 4 -NNN,d 4 -NAT, d 4 -NAB,d 4 -Methanol solution with NNK concentration of 1 μg / mL was used as the internal standard of TSNAs. Ultrasonic extraction was performed on the Cambridge filter, and the extraction time was 30 min.

[0029] The extr...

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