A kind of zinc ion sensor molecule and its preparation and application

Abstract

The invention discloses a zinc ion sensor molecule, which belongs to the field of cation detection. The sensor molecules of the present invention contain hydrazone and imine functional groups, based on their strong complexation to metal cations, which hinder excited state intramolecular proton transfer (ESIPT) and -HC=N-isomerization processes, resulting in the opening of fluorescence and Identify and detect Zn2+. Experiments show that the sensor molecule in the HEPES buffer system of DMSO / H2O, only the addition of Zn2+ makes the system emit a significant yellow-green fluorescence under ultraviolet light, and in the corresponding fluorescence spectrum, only the addition of Zn2+ makes the buffer system of the sensor molecule The maximum fluorescence emission peak appears at 524nm. Therefore, the sensor molecule can be used for the rapid fluorescence detection of Zn2+, and its minimum detection limit can reach 1.39×10‑8M, and the recognition of Zn2+ by other cations has no serious interference.

Description

technical field

[0001] The invention belongs to the field of cation detection, and relates to the preparation of a zinc ion sensor molecule; the invention also relates to the application of the sensor molecule in highly selective fluorescent recognition of zinc ions. Background technique

[0002] Zinc ions play a very important role in catalysis and structure, can contain biological enzymes and bind DNA proteins. However, an imbalance in the level of zinc ions in the body may lead to some serious neurological diseases, such as Alzheimer's disease, cerebral ischemia and epilepsy, developmental defects or disabilities. Therefore, people pay more and more attention and are committed to the development and research of efficient and highly selective detection of Zn 2+ methods, such as inductively coupled plasma atomic emission spectrometry, atomic absorption spectrometry, electrochemical methods, etc. However, most of these methods require complex electrical instruments and ted...

Examples

Embodiment Construction

[0040] The structure and synthesis of the sensor molecule of the present invention, the fluorescence recognition method for zinc ions, the preparation and application of the detection test paper will be described in detail below through specific examples.

[0041] 1. Synthesis and characterization of sensor molecules

[0042] In a 250mL round-bottomed flask, add 0.7500g (5mmol) of hydroxyisophthalaldehyde, 0.5995g (5.5mmol) of 2-hydrazinopyridine, 5mL (0.05mmol) of glacial acetic acid for catalyst, 30mL of ethanol solvent, and stir at reflux at 80°C After 6 hours, a brown-yellow precipitate precipitated out, and the crude product was recrystallized from ethanol to obtain a dark-yellow product, which is the sensor molecule Y, with a yield of 86.2% (m.p. > 300°C). Its structure is characterized as follows:

[0043] 1 H NMR (DMSO– d 6 , 400 MHz) δ: 11.01(s, 1H, O-H); δ: 10.80 (s, 1H, N–H);δ: 10.72 (s, 1H, N–H); δ: 8.31 (s, 1H, CH =N). IR (KBr) ν: 1622 cm -1 (-HC=N-),3422 c...

technical field

[0001] The invention belongs to the field of cation detection, and relates to the preparation of a zinc ion sensor molecule; the invention also relates to the application of the sensor molecule in highly selective fluorescent recognition of zinc ions. Background technique

[0002] Zinc ions play a very important role in catalysis and structure, can contain biological enzymes and bind DNA proteins. However, an imbalance in the level of zinc ions in the body may lead to some serious neurological diseases, such as Alzheimer's disease, cerebral ischemia and epilepsy, developmental defects or disabilities. Therefore, people pay more and more attention and are committed to the development and research of efficient and highly selective detection of Zn 2+ methods, such as inductively coupled plasma atomic emission spectrometry, atomic absorption spectrometry, electrochemical methods, etc. However, most of these methods require complex electrical instruments and ted...

Examples

Embodiment Construction

[0040] The structure and synthesis of the sensor molecule of the present invention, the fluorescence recognition method for zinc ions, the preparation and application of the detection test paper will be described in detail below through specific examples.

[0041] 1. Synthesis and characterization of sensor molecules

[0042] In a 250mL round-bottomed flask, add 0.7500g (5mmol) of hydroxyisophthalaldehyde, 0.5995g (5.5mmol) of 2-hydrazinopyridine, 5mL (0.05mmol) of glacial acetic acid for catalyst, 30mL of ethanol solvent, and stir at reflux at 80°C After 6 hours, a brown-yellow precipitate precipitated out, and the crude product was recrystallized from ethanol to obtain a dark-yellow product, which is the sensor molecule Y, with a yield of 86.2% (m.p. > 300°C). Its structure is characterized as follows:

[0043] 1 H NMR (DMSO– d 6 , 400 MHz) δ: 11.01(s, 1H, O-H); δ: 10.80 (s, 1H, N–H);δ: 10.72 (s, 1H, N–H); δ: 8.31 (s, 1H, CH =N). IR (KBr) ν: 1622 cm -1 (-HC=N-),3422 c...

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