Tissue engineering material with biologically active surface layer and preparation method thereof

Abstract

The invention discloses a tissue engineering material with a biologically active surface layer and a preparation method thereof. The tissue engineering material comprises a biodegradable high polymer material matrix and a dopamine layer adsorbed on the surface of the matrix, wherein the dopamine layer is connected with a biologically active polymer. The preparation method comprises the following steps: providing the biodegradable high polymer material matrix with a thoroughly wetted surface; providing a buffer system with dissolved dopamine and the biologically active polymer; and fully soaking the matrix in the buffer system, carrying out incubation at 30 to 40 DEG C for 1 to 5 h, then taking the matrix out and subjecting the matrix to air drying so as to obtain the tissue engineering material with the biologically active surface layer. The tissue engineering material has high mechanical properties and controllable degradation performance and good biological activity and is beneficial for adherence and propagation of cells; and the preparation method is simple, easy to operate, low in cost and favorable for large-scale implementation.

Description

technical field

[0001] The invention relates to a tissue engineering material, in particular to a tissue engineering material with biological activity on the surface and a preparation method thereof, belonging to the technical field of biopolymer medical materials. technical background

[0002] Polymer materials and their composites are widely used in modern medicine, and such materials are usually called medical polymer materials. Among them, a class of polymer materials that can be degraded or enzymatically hydrolyzed in the living body, and the resulting small molecular substances are absorbed by the body and excreted from the body, that is, biodegradable materials have become the mainstream of development. There are many kinds of biodegradable polymers, which can be divided into two categories according to the source: natural and synthetic, with large differences in properties and a wide range of applications. Ideal biodegradable materials should generally have good bio...

Examples

Embodiment 1

[0019] Example 1: This embodiment relates to a tissue engineering material with biologically active groups, the base material of which is polylactic acid (PLA), and chitin active polymer is linked via dopamine.

[0020] The preparation method of the tissue engineering material comprises the following steps:

[0021] Step 1. Disinfect the PLA matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 2 days, and distilled water for 1.5 days to make it fully humidified;

[0022] Step 2, preparing the modification liquid solution, dissolving dopamine in Tris buffer solution, carrying out activation treatment, then adding chitin active polymer to make it fully dissolved and mixed evenly, wherein the concentration of dopamine is 2.5mg / ml, and the concentration of chitin is 3mg / ml;

[0023] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix ma...

Embodiment 2

[0026] Example 2: This example relates to a tissue engineering material with biologically active groups, the base material of which is poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV), and chitin active polymer is linked by dopamine .

[0027] The preparation method of the tissue engineering material comprises the following steps:

[0028] Step 1. Disinfect the PHBV matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;

[0029] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add gene peptide gel active polymer to fully dissolve and mix evenly. The concentration of dopamine is 3mg / ml, and the gene peptide Glue concentration is 3mg / ml;

[0030] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matri...

Embodiment 3

[0033] Example 3: This embodiment relates to a tissue engineering material with bioactive groups, the base material of which is polycaprolactone (PCL), and chitin active polymer is connected via dopamine.

[0034] The preparation method of the tissue engineering material comprises the following steps:

[0035] Step 1. Sterilize the PCL matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;

[0036] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add type I collagen active polymer to fully dissolve and mix evenly, wherein the concentration of dopamine is 4mg / ml, and type I collagen gel The concentration is 4mg / ml;

[0037] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix material, and incubat...

technical field

[0001] The invention relates to a tissue engineering material, in particular to a tissue engineering material with biological activity on the surface and a preparation method thereof, belonging to the technical field of biopolymer medical materials. technical background

[0002] Polymer materials and their composites are widely used in modern medicine, and such materials are usually called medical polymer materials. Among them, a class of polymer materials that can be degraded or enzymatically hydrolyzed in the living body, and the resulting small molecular substances are absorbed by the body and excreted from the body, that is, biodegradable materials have become the mainstream of development. There are many kinds of biodegradable polymers, which can be divided into two categories according to the source: natural and synthetic, with large differences in properties and a wide range of applications. Ideal biodegradable materials should generally have good bio...

Examples

Embodiment 1

[0019] Example 1: This embodiment relates to a tissue engineering material with biologically active groups, the base material of which is polylactic acid (PLA), and chitin active polymer is linked via dopamine.

[0020] The preparation method of the tissue engineering material comprises the following steps:

[0021] Step 1. Disinfect the PLA matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 2 days, and distilled water for 1.5 days to make it fully humidified;

[0022] Step 2, preparing the modification liquid solution, dissolving dopamine in Tris buffer solution, carrying out activation treatment, then adding chitin active polymer to make it fully dissolved and mixed evenly, wherein the concentration of dopamine is 2.5mg / ml, and the concentration of chitin is 3mg / ml;

[0023] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix ma...

Embodiment 2

[0026] Example 2: This example relates to a tissue engineering material with biologically active groups, the base material of which is poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV), and chitin active polymer is linked by dopamine .

[0027] The preparation method of the tissue engineering material comprises the following steps:

[0028] Step 1. Disinfect the PHBV matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;

[0029] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add gene peptide gel active polymer to fully dissolve and mix evenly. The concentration of dopamine is 3mg / ml, and the gene peptide Glue concentration is 3mg / ml;

[0030] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matri...

Embodiment 3

[0033] Example 3: This embodiment relates to a tissue engineering material with bioactive groups, the base material of which is polycaprolactone (PCL), and chitin active polymer is connected via dopamine.

[0034] The preparation method of the tissue engineering material comprises the following steps:

[0035] Step 1. Sterilize the PCL matrix material, then place it in the culture plate, and then treat it with absolute alcohol for 3 days, and distilled water for 1 day to make it fully humidified;

[0036] Step 2. Prepare the modification liquid solution, dissolve dopamine in Tris buffer, perform activation treatment, and then add type I collagen active polymer to fully dissolve and mix evenly, wherein the concentration of dopamine is 4mg / ml, and type I collagen gel The concentration is 4mg / ml;

[0037] Step 3. Add the modification solution prepared in step 2) to the culture plate on which the matrix material was placed in step 1), fully soak the matrix material, and incubat...