Application of bacillus subtilis to degradation of cellulose in tobacco products

A technology of Bacillus subtilis and tobacco products, which is applied in the application field of Bacillus subtilis xp strain in degrading cellulose in tobacco products, which can solve the problems of cigarette quality, side effects, bad smell, etc., so as to improve the grade of tobacco and improve the effect of smoking evaluation , The effect of simple operation process

CN106723311AActive Publication Date: 2017-05-31CHINA TOBACCO HENAN IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-05-31

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Abstract

The invention belongs to the technical field of tobacco preparation, in particular to application of a bacillus subtilis xp bacterial strain to degradation of cellulose in tobacco products. The bacillus subtilis xp bacterial strain is currently preserved in the China Center For Type Culture Collection, and a preservation number is CCTCC NO:M 2011452. The bacillus subtilis xp bacterial strain is used for reducing the cellulose content of the tobacco products. In application, the bacillus subtilis xp bacterial strain is made into a bacterial agent, the bacterial agent is uniformly sprayed to surfaces of the tobacco products according to a tobacco product mass ratio of 1.2%, and then storage for mellowing is performed for 40-50d under conditions that the temperature is 30-35DEG C, and humidity is 55-65%. By selection of the specific bacillus subtilis bacterial strain, effectiveness in reduction of the cellulose content of the tobacco products can be proved, tobacco grade is raised, and a tobacco mellowing process is accelerated while mellowing effects are guaranteed.
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Description

Technical field

[0001] The invention belongs to the technical field of tobacco preparation, and specifically is an application of a Bacillus subtilis xp strain in degrading cellulose in tobacco products. Background technique

[0002] Bacillus subtilis is a kind of Bacillus. It is widely distributed in soil and decaying organic matter. It is easy to multiply in subtilis infusion. The surface of the colony is rough and opaque, dirty white or slightly yellow. It can use protein, various sugars and starches to decompose tryptophan to form indole, which is widely used in genetic research. In addition, Bacillus subtilis itself can synthesize enzymes such as α-amylase, neutral protease, lipase, cellulase, etc., and has good application prospects in degrading complex macromolecular compounds. Therefore, Bacillus subtilis has gradually become The focus of attention and research.

[0003] Tobacco leaf fermentation is also known as tobacco leaf aging. Its essence is the process of profound ...

Examples

Embodiment

[0024] In order to use the Bacillus subtilis xp strain to degrade cellulose in tobacco products, the strain must first be properly amplified and cultured and prepared to contain a certain amount of active inoculum before it can be used. The specific preparation method of the inoculum is as follows.

[0025] (1) Strain activation, use the inoculating loop to pick the slant medium (the formula is the same as LB liquid medium, only add 15g / L agar powder) on the strain, place it in LB liquid medium for suspension culture, cultivate at 30℃ for 12h Carry out strain activation;

[0026] (2) Strain amplification, transfer the bacterial solution after the strain activation in step (1) to LB liquid culture solution, and cultivate to OD on a shaker at 35°C 600 = 3.5, it can be used as a fungicide for degrading tobacco products after being evenly suspended.

[0027] When the above fungus is specifically used to degrade cellulose in tobacco products, spray it evenly on the surface of the tobacco ...