Extraction method for deoxynojirimycin

Abstract

The invention provides an extraction method for deoxynojirimycin. The method comprises the following steps: mixing a mulberry raw material with water, then carrying out ultrasonic treatment and solid-liquid separation and collecting clear liquid; subjecting the obtained clear liquid to affinity chromatography, carrying out elution by using a desorption solution and collecting eluate; and concentrating the obtained eluate and drying obtained concentrate so as to obtain deoxynojirimycin. Deoxynojirimycin extracted by using the method has high purity; and the method is friendly to environment, easy for scaling up, simple in process and suitable for industrial production.

Description

technical field

[0001] The invention relates to the field of separation and purification of active ingredients of natural products, in particular to a method for extracting deoxynojirimycin. Background technique

[0002] Deoxynojirimycin (DNJ) is a polyhydroxypiperidine alkaloid present in mulberry trees and other plants and microorganisms, the chemical name is 3,4,5-trihydroxy-2-hydroxymethyltetrahydropyridine, Molecular formula is C 6 h 13 NO 4 , with a molecular weight of 163, has a structure similar to α-1,4-glucose, is a potent α-glucosidase inhibitor, has hypoglycemic function, and also has anti-tumor and anti-viral activities. Natural deoxynojirimycin has become an internationally recognized biological agent raw material for clinical treatment of diabetes, with high medicinal value and promising application prospects.

[0003] However, there are many difficulties in the separation and purification of deoxynojirimycin. Most of the existing separation and purificati...

Examples

Embodiment 1

[0037] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0038] (1) Mix 1 kg of mulberry leaves with crushed water of 5 times the mass, ultrasonicate 3 times at 50°C for a total of 60 minutes, filter, and collect the supernatant;

[0039] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0040] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.

Embodiment 2

[0042] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0043](1) Mix 1 kg of mulberry branches with crushed water of 50 times the mass, ultrasonicate 5 times at 80°C for a total of 30 minutes, filter, and collect the supernatant;

[0044] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0045] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.

Embodiment 3

[0047] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0048] (1) Mix 1 kg of mulberry bark with crushed water of 50 times the mass, ultrasonicate 5 times at 80°C for a total of 60 minutes, filter, and collect the supernatant;

[0049] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0050] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.

technical field

[0001] The invention relates to the field of separation and purification of active ingredients of natural products, in particular to a method for extracting deoxynojirimycin. Background technique

[0002] Deoxynojirimycin (DNJ) is a polyhydroxypiperidine alkaloid present in mulberry trees and other plants and microorganisms, the chemical name is 3,4,5-trihydroxy-2-hydroxymethyltetrahydropyridine, Molecular formula is C 6 h 13 NO 4 , with a molecular weight of 163, has a structure similar to α-1,4-glucose, is a potent α-glucosidase inhibitor, has hypoglycemic function, and also has anti-tumor and anti-viral activities. Natural deoxynojirimycin has become an internationally recognized biological agent raw material for clinical treatment of diabetes, with high medicinal value and promising application prospects.

[0003] However, there are many difficulties in the separation and purification of deoxynojirimycin. Most of the existing separation and purificati...

Examples

Embodiment 1

[0037] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0038] (1) Mix 1 kg of mulberry leaves with crushed water of 5 times the mass, ultrasonicate 3 times at 50°C for a total of 60 minutes, filter, and collect the supernatant;

[0039] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0040] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.

Embodiment 2

[0042] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0043](1) Mix 1 kg of mulberry branches with crushed water of 50 times the mass, ultrasonicate 5 times at 80°C for a total of 30 minutes, filter, and collect the supernatant;

[0044] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0045] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.

Embodiment 3

[0047] A method for extracting deoxynojirimycin, said method comprising the following steps:

[0048] (1) Mix 1 kg of mulberry bark with crushed water of 50 times the mass, ultrasonicate 5 times at 80°C for a total of 60 minutes, filter, and collect the supernatant;

[0049] (2) Perform affinity chromatography on the clear liquid obtained in step (1) using a glucosidase affinity chromatography column, use ethanol to elute, and collect the eluate;

[0050] (3) Concentrating the eluate obtained in step (2) by distillation under reduced pressure, recovering ethanol, and freeze-drying the concentrated solution to obtain deoxynojirimycin.