Application of oxymatrine to preparation of medicine for resisting osteoclast-mediated bone loss

Abstract

The invention discloses application of oxymatrine to preparation of a medicine for resisting osteoclast-mediated bone loss. According to the application, the oxymatrine is innovatively used for treating osteoclast-mediated osteolytic diseases and an inhibition effect of the oxymatrine on osteoclast differentiation is explained for the first time at a cellular level. Meanwhile, a bone and joint unstable model is manufactured by applying anterior cruciate ligament transection; the model has mouse knee joint subchondral bone loss and cells with an osteolysis effect are osteoclasts; an animal experiment result shows that the osteoclast differentiation-mediated subchondral bone loss can be remarkably reduced through intraperitoneal injection of the oxymatrine and the medicine has a remarkable protection effect on mouse joints. Finally, under in vivo conditions, the invention proves that the oxymatrine has no remarkable toxin on visceral organs in a whole body and an ideal new option is provided for treatment of the osteolytic diseases.

Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to the application of oxymatrine in the preparation of anti-osteoclast mediated bone loss medicine. Background technique

[0002] Osteoblasts and osteoclasts are the two most important cells in bone remodeling to maintain bone mass. Osteoclasts are the only cells that absorb bone in the human body. Under physiological conditions, they maintain the metabolic balance of normal bone tissue together with osteoblasts. When the number of osteoclasts increases or the bone resorption function increases, it can cause a variety of common diseases caused by excessive loss of bone mass, including osteoporosis, osteoarthritis, tumor bone metastasis and artificial prosthesis loosening. In my country, more than 200 million people suffer from osteoporosis, and its incidence rate increases rapidly with the aging of our society. The main pathological process leading to osteoporosis is the incre...

Examples

Embodiment 1

[0036] Example 1 Effect of Oxymatrine on Osteolysis Mediated by Osteoclasts and Its Mechanism

[0037] (1) Materials and methods

[0038] 1. Extraction and culture of mouse bone marrow macrophages

[0039] C57 mice aged 2-4 weeks were killed by cervical dislocation, and bilateral femurs and tibias were removed and transferred to a clean table. Carefully separate muscle and other tissues to fully expose the bone, remove the full-length tibia and femur of the mouse, rinse with PBS 3 times, 3 minutes each time, use sterile scissors to separate the two ends of the long bone, and rinse the bone marrow with a 1ml needle until filled with In the α-MEM medium containing 10% fetal bovine serum, during the flushing process, move the needle up and down to scrape the bone marrow cavity. Use approximately 5ml of medium per bone and discard the bone after flushing is complete. The cell suspension was centrifuged (1000 rpm / 3 min). Discard the supernatant, add culture medium (add 10 ng / ml...

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to the application of oxymatrine in the preparation of anti-osteoclast mediated bone loss medicine. Background technique

[0002] Osteoblasts and osteoclasts are the two most important cells in bone remodeling to maintain bone mass. Osteoclasts are the only cells that absorb bone in the human body. Under physiological conditions, they maintain the metabolic balance of normal bone tissue together with osteoblasts. When the number of osteoclasts increases or the bone resorption function increases, it can cause a variety of common diseases caused by excessive loss of bone mass, including osteoporosis, osteoarthritis, tumor bone metastasis and artificial prosthesis loosening. In my country, more than 200 million people suffer from osteoporosis, and its incidence rate increases rapidly with the aging of our society. The main pathological process leading to osteoporosis is the incre...

Examples

Embodiment 1

[0036] Example 1 Effect of Oxymatrine on Osteolysis Mediated by Osteoclasts and Its Mechanism

[0037] (1) Materials and methods

[0038] 1. Extraction and culture of mouse bone marrow macrophages

[0039] C57 mice aged 2-4 weeks were killed by cervical dislocation, and bilateral femurs and tibias were removed and transferred to a clean table. Carefully separate muscle and other tissues to fully expose the bone, remove the full-length tibia and femur of the mouse, rinse with PBS 3 times, 3 minutes each time, use sterile scissors to separate the two ends of the long bone, and rinse the bone marrow with a 1ml needle until filled with In the α-MEM medium containing 10% fetal bovine serum, during the flushing process, move the needle up and down to scrape the bone marrow cavity. Use approximately 5ml of medium per bone and discard the bone after flushing is complete. The cell suspension was centrifuged (1000 rpm / 3 min). Discard the supernatant, add culture medium (add 10 ng / ml...

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