Method for earthing-cultivating silkworm insect grass by simulating ecological environment

Abstract

The invention discloses a method for earthing-cultivating silkworm insect grass by simulating an ecological environment. The method comprises the following steps: (1) selecting a strain host; (2) enlarging preparation solution of cultivated strain liquid; (3) preprocessing and postprocessing inoculation; (4) preparing an embedding substrate; and (5) management of earthing-cultivating: inoculatingthe strains optimized in the step (2) in a fluid nutrient medium added with 5-8 wt% of silkworm chrysalis meal, after enlarging the cultivation, inoculating in healthy silkworm larvae in the step (1)for infecting and rigidifying, and embedding infected and rigidified silkworm bodies to be developed in the step (3) in a transparent plastic box filled with the plant ash embedding substrate; after sporophore is grown to be matured, taking out the silkworm bodies from the plant ash embedding substrate, and cleaning up plant ash adhered to the periphery of the silkworm bodies, to obtain the matured silkworm insect grass. The method has the characteristics of low sundry fungus contamination rate, high infection rigidifying rate, high production rate, good stability of the obtained silkworm insect grass, higher yield, low cost, and full and regular appearance of the silkworm insect grass, is suitable for the large-scale production, and has the extensive development prospect.

Description

technical field

[0001] The invention relates to a method for cultivating silkworm caterpillar fungus by covering soil in a simulated ecological environment, and belongs to the technical field of cordyceps cultivation. Background technique

[0002] Cordyceps is a class of medicinal and edible fungi with significant health functions. The most famous of them are Cordyceps sinensis, Cordyceps militaris and Cicada. Taking Lepidoptera insects as hosts, infecting insect larvae in different ways, and breaking through the insect body surface through continuous growth and development, a fungus-fungus complex that coexists with the shell of the insect body is formed. The medicinal value of Cordyceps militaris is recorded in the medical bibliographies such as "Chinese Medicine Sea" and "Xinhua Compendium of Materia Medica". Its medicinal properties are mild and have a wide range of medicinal and edible properties. It can be eaten all year round. It is suitable for those who are young, s...

Examples

Embodiment 1

[0030] This embodiment includes the following steps:

[0031] (1) Selection of Cordyceps militaris host: Select the larvae of the original silkworm species (7 Xiang) on ​​the 4th day of the 5th instar. After the 5th instar was fed, they were continuously fed to leaves at a temperature of 25°C and a relative humidity of 75%. Remove sand to ensure the health and cleanliness of the silkworm body, and select healthy silkworm larvae for backup;

[0032] (2) Liquid preparation solution for expanded culture strains: after being activated on the plate medium of Cordyceps militaris mother species that grows well on silkworm chrysalis, the strains are evenly divided, and inoculated in a liquid medium supplemented with 5wt% silkworm chrysalis powder, under dark conditions Cultivate statically at 20°C for 2 days, start shaking culture on the 3rd day, shake 3 hours a day, 135r / min, cultivate 1d at 200lx light intensity on the 5th day, remove mycelium by filtering with 4 layers of sterile g...

Embodiment 2

[0042] This embodiment includes the following steps:

[0043] (1) Selection of the host of Cordyceps militaris: select the larvae of the first-generation hybrid silkworm (9 Fu X7 Xiang) of the fifth instar 4d, after the 5th instar feeds, under the environmental conditions of a temperature of 25°C and a relative humidity of 75%, the larvae are continuously fed to the leaves, and timely Remove sand to ensure the health and cleanliness of the silkworm body, and select healthy silkworm larvae for backup;

[0044] (2) Liquid preparation solution for expanded culture strains: after being activated on the plate medium of Cordyceps militaris mother species that grows well on silkworm chrysalis, the strains are evenly divided, and inoculated in a liquid medium supplemented with 8wt% silkworm chrysalis powder, in the dark Conditions, temperature 20°C for static culture for 2 days, start shaking culture on the 3rd day, shake for 3 hours every day, 135r / min, culture at 200lx light intensi...

Embodiment 3

[0054] This embodiment includes the following steps:

[0055] (1) Selection of the host of Cordyceps militaris: the larvae of the first-generation hybrid of silkworm (9 Fu X7 Xiang) of the fifth instar 6d were selected. After feeding at the 5th instar, under the environmental conditions of temperature 25°C and relative humidity 75%, feed leaves continuously, remove sand in time to ensure the health and cleanness of silkworm bodies, and select healthy silkworm larvae for use;

[0056] (2) Liquid preparation solution for expanded culture strains: select and activate the Cordyceps militaris mother seed plate medium that grows well on silkworm chrysalis, divide the strains evenly, and inoculate them in a liquid medium supplemented with 8% silkworm chrysalis powder, in the dark Conditions and temperature: 20°C for static culture for 2 days, start shaking culture on the 3rd day, shake every day for 3 hours, 135r / min, and culture for 1 day under the light intensity of 200lx on the 5t...

technical field

[0001] The invention relates to a method for cultivating silkworm caterpillar fungus by covering soil in a simulated ecological environment, and belongs to the technical field of cordyceps cultivation. Background technique

[0002] Cordyceps is a class of medicinal and edible fungi with significant health functions. The most famous of them are Cordyceps sinensis, Cordyceps militaris and Cicada. Taking Lepidoptera insects as hosts, infecting insect larvae in different ways, and breaking through the insect body surface through continuous growth and development, a fungus-fungus complex that coexists with the shell of the insect body is formed. The medicinal value of Cordyceps militaris is recorded in the medical bibliographies such as "Chinese Medicine Sea" and "Xinhua Compendium of Materia Medica". Its medicinal properties are mild and have a wide range of medicinal and edible properties. It can be eaten all year round. It is suitable for those who are young, s...

Examples

Embodiment 1

[0030] This embodiment includes the following steps:

[0031] (1) Selection of Cordyceps militaris host: Select the larvae of the original silkworm species (7 Xiang) on ​​the 4th day of the 5th instar. After the 5th instar was fed, they were continuously fed to leaves at a temperature of 25°C and a relative humidity of 75%. Remove sand to ensure the health and cleanliness of the silkworm body, and select healthy silkworm larvae for backup;

[0032] (2) Liquid preparation solution for expanded culture strains: after being activated on the plate medium of Cordyceps militaris mother species that grows well on silkworm chrysalis, the strains are evenly divided, and inoculated in a liquid medium supplemented with 5wt% silkworm chrysalis powder, under dark conditions Cultivate statically at 20°C for 2 days, start shaking culture on the 3rd day, shake 3 hours a day, 135r / min, cultivate 1d at 200lx light intensity on the 5th day, remove mycelium by filtering with 4 layers of sterile g...

Embodiment 2

[0042] This embodiment includes the following steps:

[0043] (1) Selection of the host of Cordyceps militaris: select the larvae of the first-generation hybrid silkworm (9 Fu X7 Xiang) of the fifth instar 4d, after the 5th instar feeds, under the environmental conditions of a temperature of 25°C and a relative humidity of 75%, the larvae are continuously fed to the leaves, and timely Remove sand to ensure the health and cleanliness of the silkworm body, and select healthy silkworm larvae for backup;

[0044] (2) Liquid preparation solution for expanded culture strains: after being activated on the plate medium of Cordyceps militaris mother species that grows well on silkworm chrysalis, the strains are evenly divided, and inoculated in a liquid medium supplemented with 8wt% silkworm chrysalis powder, in the dark Conditions, temperature 20°C for static culture for 2 days, start shaking culture on the 3rd day, shake for 3 hours every day, 135r / min, culture at 200lx light intensi...

Embodiment 3

[0054] This embodiment includes the following steps:

[0055] (1) Selection of the host of Cordyceps militaris: the larvae of the first-generation hybrid of silkworm (9 Fu X7 Xiang) of the fifth instar 6d were selected. After feeding at the 5th instar, under the environmental conditions of temperature 25°C and relative humidity 75%, feed leaves continuously, remove sand in time to ensure the health and cleanness of silkworm bodies, and select healthy silkworm larvae for use;

[0056] (2) Liquid preparation solution for expanded culture strains: select and activate the Cordyceps militaris mother seed plate medium that grows well on silkworm chrysalis, divide the strains evenly, and inoculate them in a liquid medium supplemented with 8% silkworm chrysalis powder, in the dark Conditions and temperature: 20°C for static culture for 2 days, start shaking culture on the 3rd day, shake every day for 3 hours, 135r / min, and culture for 1 day under the light intensity of 200lx on the 5t...

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