A reporter gene system for screening biofilm inhibitors

A biofilm and reporter gene technology, applied in the field of bioengineering, can solve the problems of reducing heat transfer, clogged pipes, and easy formation of biofilm pollution sources, and achieve the effect of reducing antibiotic resistance

CN108300723BActive Publication Date: 2022-04-08TIANJIN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-04-08

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Abstract

The present invention relates to a novel reporter gene system for screening biofilm inhibitors. The application proves that the bsrA gene can inhibit the formation of Pseudomonas aeruginosa biofilm, and its coding gene is overexpressed in bacteria, which can significantly inhibit the formation of biofilm Yield. Therefore, using this gene as a marker, a new reporter gene system is constructed to screen small molecular compounds that can activate the expression of the marker gene, which can inhibit the synthesis of biofilm from the gene level and help to solve the problem caused by biofilm. Persistent infection, antibiotic resistance, and at the same time, by studying the mechanism of this gene to inhibit the formation of biofilm, we can better solve the problem of microbial biofilm formation mechanism and the inhibition and removal of biofilm in the food processing industry.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, in particular, the invention relates to the application of a regulation gene in inhibiting microorganism biofilm (biofilm). By constructing a fusion reporter gene system to screen a small molecular compound to activate the screening marker gene, the expression of the marker gene is increased, thereby inhibiting the biofilm synthesis. It is of great significance for the multi-drug resistance caused by Pseudomonas aeruginosa and the food-borne diseases caused by biofilm. Background technique

[0002] At present, the treatment of nosocomial infection mainly uses antibiotics to kill or inhibit the growth of pathogenic bacteria. However, Pseudomonas aeruginosa itself has some drug resistance mechanisms, including the outer membrane with low permeability, the expression of constitutive genes of different efflux pumps, and the production of antibiotic inactivating enzymes (such as cephalosporinase), etc.,...

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Embodiment Construction

[0027] The following descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form; all those skilled in the art can implement the present invention smoothly according to the above description; Without departing from the scope of the technical solution of the present invention, professional technicians can use the technical content disclosed above to make some changes, modifications and equivalent changes, which are all equivalent embodiments of the present invention.

[0028] In the present invention, the gene bsrA is used as a marker gene, and a gene reporter system is constructed to screen small molecular compounds that promote marker gene expression.