Preparation method and application of injectable allogeneic adipose acellular matrix microparticles

Abstract

The invention discloses a preparation method and application of injectable allogeneic fat decellularized matrix microparticles. The adipose tissue obtained from liposuction is subjected to mechanical chylolysis, centrifugation to remove oil, subsequent gentle decellularization process, and centrifugation. Obtained by further degreasing; among them, the adipose tissue is derived from the human-derived fat particles discarded in the liposuction operation of traditional obese patients. The present invention adopts the method of physically crushing fat cells and degreasing methods as well as the technology of gentle decellularization, which is easy to operate and has no effect on fat loss. The matrix structure and composition are less damaged, and the obtained material is closer to the microenvironment of human natural adipose tissue, retaining the three-dimensional structure and active ingredients of the tissue, and can be injected with a small needle of 27g, which is more conducive to the proliferation and differentiation of tissue cells in the recipient area after injection , so as to promote the repair and regeneration of tissues, and play a long-term filling and rejuvenating effect. The product of the invention is suitable for facial rejuvenation and repair of soft tissue defects.

Description

technical field

[0001] The invention relates to the field of medical plastic cosmetic materials, in particular to a preparation method and application of injectable allogeneic adipose acellular matrix particles. Background technique

[0002] With the development of society, the improvement of people's living standards and the popularization of micro-plastic surgery in recent years, the application of injection filling materials has greatly increased in international and domestic demand. Currently, hyaluronic acid is commonly used as an injection filling material. It has obvious advantages, but it also has the problem of degradation and absorption, and it cannot stimulate tissue regeneration, so it cannot fundamentally solve the problem of rejuvenation.

[0003] Non-surgical or minimally invasive treatment is one of the development directions of plastic surgery. Filling treatment is often the first choice for facial fine wrinkles or volume loss. Filling of soft tissues of the...

Examples

preparation example Construction

[0048] A method for preparing injectable allogeneic adipose acellular matrix microparticles of the present invention comprises the following steps:

[0049] (1) Fat collection and purification;

[0050] Obtain human-derived fat particles through liposuction, wash the obtained fat particles twice with rinsing solution to remove red blood cells in the fat tissue, collect the fat particles in a syringe, and perform centrifugation;

[0051] Discard the upper layer of grease and the lower layer of liquid, and collect the fat particles in the middle;

[0052] (2) Chylification treatment;

[0053] The fat particles obtained in step (1) are subjected to mechanical chylolysis, centrifuged to remove oil, and made into fat particles;

[0054] (3) Decellularization treatment;

[0055] Remove cell debris and antigens from the fat particles obtained in step (2), and further degrease and degrease through rinsing and centrifugation to obtain fat acellular matrix particles;

[0056] (4) Dr...

Embodiment 1

[0062] A preparation method of injectable allogeneic adipose acellular matrix microparticles of the present invention mainly comprises the following steps:

[0063] (1) Fat collection and purification treatment: select patients who need liposuction, apply mechanical negative pressure, manual negative pressure of syringe, hydrodynamic assisted liposuction, vibration assisted liposuction and other methods to remove fat accumulation parts (upper arms, shoulders, Abdominal, waist, buttocks, thighs, etc.) for liposuction, wash the fat particles twice with sterile distilled water to remove red blood cells in the fat tissue, collect the fat particles in a 50ml or larger centrifuge tube, and centrifuge at 3000rpm 12min; Discard the upper layer of fat and the lower layer of liquid, and collect the fat particles in the middle;

[0064] Fat is the most abundant tissue type in the human body. It has abundant sources and can be obtained through liposuction. Human-derived adipose acellular ...

Embodiment 2

[0072] The preparation method of an injectable allogeneic adipose acellular matrix particle of the present invention mainly comprises the following steps:

[0073] (1) Fat collection and purification: select the abdominal area of ​​the patient for traditional negative pressure-assisted liposuction, wash the fat particles three times with sterile distilled water, collect the fat particles in a 50ml or larger centrifuge tube, and centrifuge at 5000rpm for 10min , discard the upper layer of fat and the lower layer of liquid, and collect the fat particles in the middle.

[0074] (2) Chylification treatment: extract fat particles with a 20ml screw-mouth syringe, connect to another 20ml screw-mouth syringe with a luer connector (emulsifier) ​​with a hole diameter of 2.4mm, or use a larger-capacity mechanical device to inject back and forth The two syringes are used about 10 to 30 times until the fat particles move smoothly in the injector. Then connect Luer connectors (emulsifiers)...

technical field

[0001] The invention relates to the field of medical plastic cosmetic materials, in particular to a preparation method and application of injectable allogeneic adipose acellular matrix particles. Background technique

[0002] With the development of society, the improvement of people's living standards and the popularization of micro-plastic surgery in recent years, the application of injection filling materials has greatly increased in international and domestic demand. Currently, hyaluronic acid is commonly used as an injection filling material. It has obvious advantages, but it also has the problem of degradation and absorption, and it cannot stimulate tissue regeneration, so it cannot fundamentally solve the problem of rejuvenation.

[0003] Non-surgical or minimally invasive treatment is one of the development directions of plastic surgery. Filling treatment is often the first choice for facial fine wrinkles or volume loss. Filling of soft tissues of the...

Examples

preparation example Construction

[0048] A method for preparing injectable allogeneic adipose acellular matrix microparticles of the present invention comprises the following steps:

[0049] (1) Fat collection and purification;

[0050] Obtain human-derived fat particles through liposuction, wash the obtained fat particles twice with rinsing solution to remove red blood cells in the fat tissue, collect the fat particles in a syringe, and perform centrifugation;

[0051] Discard the upper layer of grease and the lower layer of liquid, and collect the fat particles in the middle;

[0052] (2) Chylification treatment;

[0053] The fat particles obtained in step (1) are subjected to mechanical chylolysis, centrifuged to remove oil, and made into fat particles;

[0054] (3) Decellularization treatment;

[0055] Remove cell debris and antigens from the fat particles obtained in step (2), and further degrease and degrease through rinsing and centrifugation to obtain fat acellular matrix particles;

[0056] (4) Dr...

Embodiment 1

[0062] A preparation method of injectable allogeneic adipose acellular matrix microparticles of the present invention mainly comprises the following steps:

[0063] (1) Fat collection and purification treatment: select patients who need liposuction, apply mechanical negative pressure, manual negative pressure of syringe, hydrodynamic assisted liposuction, vibration assisted liposuction and other methods to remove fat accumulation parts (upper arms, shoulders, Abdominal, waist, buttocks, thighs, etc.) for liposuction, wash the fat particles twice with sterile distilled water to remove red blood cells in the fat tissue, collect the fat particles in a 50ml or larger centrifuge tube, and centrifuge at 3000rpm 12min; Discard the upper layer of fat and the lower layer of liquid, and collect the fat particles in the middle;

[0064] Fat is the most abundant tissue type in the human body. It has abundant sources and can be obtained through liposuction. Human-derived adipose acellular ...

Embodiment 2

[0072] The preparation method of an injectable allogeneic adipose acellular matrix particle of the present invention mainly comprises the following steps:

[0073] (1) Fat collection and purification: select the abdominal area of ​​the patient for traditional negative pressure-assisted liposuction, wash the fat particles three times with sterile distilled water, collect the fat particles in a 50ml or larger centrifuge tube, and centrifuge at 5000rpm for 10min , discard the upper layer of fat and the lower layer of liquid, and collect the fat particles in the middle.

[0074] (2) Chylification treatment: extract fat particles with a 20ml screw-mouth syringe, connect to another 20ml screw-mouth syringe with a luer connector (emulsifier) ​​with a hole diameter of 2.4mm, or use a larger-capacity mechanical device to inject back and forth The two syringes are used about 10 to 30 times until the fat particles move smoothly in the injector. Then connect Luer connectors (emulsifiers)...