A device and testing method for imaging the transparent droplet in light sheet fluorescence microscopy

A microscopic imaging and transparent technology, applied in biochemical equipment and methods, measurement devices, fluorescence/phosphorescence, etc., can solve problems such as low detection throughput, and achieve increased field of view, reduced volume, and improved laser energy utilization. Effect

CN109060736AInactive Publication Date: 2018-12-21因纽美瑞克斯公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2018-12-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a device and testing method for imaging the transparent droplet in light sheet fluorescence microscopy. The imaging device comprises a light source reshaping module, a light sheet generating module, a sample control module and an image acquisition module. The light source reshaping module converts the circular light spot into elliptical light spot; the light sheet generating module produces a sheet beam from the elliptical spot; the sample control module controls the sample to move in direction vertical to the optical axis when the sheet beam illuminates on the sample,and the image acquisition module acquires the fluorescence signal excited from the moving sample at different positions to obtain the sequential three-dimensional images of the sample. The invented device can generate an elliptical light spot in short optical path to form a bright and thick light sheet, which makes the beam shape more suitable for enclosed in-situ imaging of liquid droplets in deep location. Meanwhile, since no silt is used for blocking the laser the utilization of laser energy increases by four times. The invention enables the enlargement of light aperture under wide view field and the reduction of the length of collecting end, with small size and high integration degree.
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Description

technical field

[0001] The invention belongs to the field of biological detection, and more specifically relates to a light-sheet fluorescence microscopic imaging device and a detection method for transparent liquid droplet imaging. Background technique

[0002] Emulsion droplets are one of the practical and rapidly developing powerful tools in the field of chemical biology. The size of emulsion droplets is usually between a few microns and hundreds of microns, and they exist stably in water-in-oil emulsions under the action of specific surfactants. Due to the limitations of current means and technical conventions, the emulsion droplets in scientific research and production are generally between 10 microns and 300 microns. Because the microemulsion droplets can uniformly disperse the sample (mostly aqueous solution) into multiple units with nearly the same volume; these units are isolated from each other to form an independent reaction space, which can greatly improve the t...

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Embodiment Construction

[0036] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0037] The present invention adopts a microemulsion drop formula with the same or similar refractive index of the water phase and the oil phase to make the microemulsion droplet transparent, so that the light can pass through the shallow layer transparent droplet to reach the deep layer droplet, which is the result of the deep layer droplet. Optical signal reading provides the prerequisite. For high-throughput reading of optical signals of transparentized droplets, the present application provides a light-sheet fluorescence microscopy imaging device for imaging transparentized droplets. By optimizi...