Application method of saponin extract serving as fish immunoenhancement

Abstract

The invention discloses an application method of saponin extract serving as a fish immunoenhancement. Specifically, panax notoginseng saponin is firstly prepared into a water-soluble solution with a proper concentration; the water-soluble solution is slowly and uniformly sprayed into feed by adopting a mist-spraying manner. Effective components are uniformly distributed in the feed and the problemthat the adding dosage of powder extract is low so that the powder extract is difficult to directly and uniformly blend into the feed is solved.

Description

technical field

[0001] The invention relates to a fish immune enhancer, in particular to a method for using a saponin extract as a fish immune enhancer. Background technique

[0002] Fish are often infected by a variety of pathogens during the breeding process. Improving the disease resistance of fish through the use of immune enhancers is an important measure to reduce the occurrence of diseases and maintain the healthy and sustainable development of the aquaculture industry.

[0003] Panax notoginseng saponin is the main active ingredient of the traditional Chinese medicine plant Panax notoginseng, which has immunoregulatory effects and can be used as an immune enhancer. However, there are problems in specific applications. 1) As for the control of the amount, if the added amount is too high, it will not only increase the cost, which is not conducive to the promotion and application in practice, but also may cause negative effects on farmed animals; if the added amount is ...

Examples

Embodiment 1

[0021] A method of using saponin extract as a fish immune enhancer,

[0022] (1) Provide notoginseng saponin extract;

[0023] (2) Dissolve the notoginseng saponin extract in phosphate buffered saline (PBS), or normal saline, or deionized water, or purified water to prepare a saponin aqueous solution with a concentration of 0.05-0.1 mg / mL;

[0024] (3) Evenly spray 100n-200nmL saponin aqueous solution per kilogram of feed, calculated by adding 10nmg of Panax notoginseng saponin extract per kilogram of feed), where n is (1, 2, 3, 4, 5);

[0025] (4) When the feed is granule feed, sprinkle an appropriate amount of feed binder evenly on the surface of the granule-based feed, then put an appropriate amount of saponin aqueous solution into the watering can, spray it slowly and evenly on the feed, mix well, and dry it indoors. Dry or dry at 60°C; when the feed is powdery compound feed, put an appropriate amount of saponin aqueous solution into the watering can, slowly and evenly sp...

technical field

[0001] The invention relates to a fish immune enhancer, in particular to a method for using a saponin extract as a fish immune enhancer. Background technique

[0002] Fish are often infected by a variety of pathogens during the breeding process. Improving the disease resistance of fish through the use of immune enhancers is an important measure to reduce the occurrence of diseases and maintain the healthy and sustainable development of the aquaculture industry.

[0003] Panax notoginseng saponin is the main active ingredient of the traditional Chinese medicine plant Panax notoginseng, which has immunoregulatory effects and can be used as an immune enhancer. However, there are problems in specific applications. 1) As for the control of the amount, if the added amount is too high, it will not only increase the cost, which is not conducive to the promotion and application in practice, but also may cause negative effects on farmed animals; if the added amount is ...

Examples

Embodiment 1

[0021] A method of using saponin extract as a fish immune enhancer,

[0022] (1) Provide notoginseng saponin extract;

[0023] (2) Dissolve the notoginseng saponin extract in phosphate buffered saline (PBS), or normal saline, or deionized water, or purified water to prepare a saponin aqueous solution with a concentration of 0.05-0.1 mg / mL;

[0024] (3) Evenly spray 100n-200nmL saponin aqueous solution per kilogram of feed, calculated by adding 10nmg of Panax notoginseng saponin extract per kilogram of feed), where n is (1, 2, 3, 4, 5);

[0025] (4) When the feed is granule feed, sprinkle an appropriate amount of feed binder evenly on the surface of the granule-based feed, then put an appropriate amount of saponin aqueous solution into the watering can, spray it slowly and evenly on the feed, mix well, and dry it indoors. Dry or dry at 60°C; when the feed is powdery compound feed, put an appropriate amount of saponin aqueous solution into the watering can, slowly and evenly sp...