Functional sericin hydrogel as well as preparation method and application thereof

A technology of sericin and hydrogel, which is applied in the field of tissue engineering, can solve the problems of low transparency, poor elasticity and easy breakage, and achieve the effects of simple preparation process, excellent affinity, and wide range of gelation time

CN110041536AActive Publication Date: 2019-07-23JIANGSU UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-07-23

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Abstract

The invention discloses functional sericin hydrogel as well as a preparation method and an application thereof. According to the method, a silkworm cocoon of a specific bombyx mori silk fibroin missing type mutant variety is adopted, a sericin water solution is extracted, ultrasonic treatment is performed, and the sericin hydrogel with new characteristics is obtained. The sericin hydrogel has hightransparency; the sericin hydrogel has a microstructure different from conventional hydrogel, hydrogel micropores are orderly arranged from large to small and from small to large; the functional sericin hydrogel has the characteristics of excellent elasticity, injectability and controllable gelling time; the sericin hydrogel has certain application value in the aspect of drug release control; thesericin hydrogel has higher affinity for cells and can support adhesion and proliferation of the cells; the sericin hydrogel and a 3D porous sericin biological scaffold obtained through freeze dryingof the sericin hydrogel can serve as extracellular matrixes to support cell growth and promote exchange of nutritional substances and can be applied to repairing of damage of multiple tissues and treatment of diseases.
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Description

technical field

[0001] The invention belongs to the field of tissue engineering and relates to a biomedical material, specifically functional sericin hydrogel and its preparation method and application. Background technique

[0002] Hydrogel is a network structure formed by a variety of intermolecular forces, including ionic bonds, hydrogen bonds, hydrophobic interactions or covalent bonds, which can accommodate a large amount of water and are easily soluble in water. Hydrogel has pores, can support water and material exchange, and its physical and chemical properties are similar to natural tissue scaffolds. As an important class of biomaterials, hydrogels have a wide range of applications in the biomedicine and skin care industries.

[0003] Among the many materials for preparing hydrogels, natural biomaterials are favored because of their characteristics of cell signal recognition, promotion of cell proliferation and differentiation, and good biocompatibility.

[0004] ...

Examples

Embodiment 1

[0041] A preparation method of sericin hydrogel, comprising the following steps:

[0042] 1. Silkworm cocoon selection

[0043]Silkworm silkworm cocoons (preserved by the Institute of Sericulture, Chinese Academy of Agricultural Sciences and provided commercially) are selected as raw materials, and the main chemical component is silkworm sericin.

[0044] 2. Extraction and separation of sericin

[0045] (1) Carry out impurity-removing treatment to the cocoons of silkworm mutant varieties, and cut into 0.2cm 2 fragments, take 1g of the shredded sericin cocoons for subsequent use;

[0046] (2) Mix the cocoon fragments obtained in step (1) with 50 mL of LiBr solution with a molar concentration of 6 mol / L, and place the mixed solution in a constant temperature water bath at 35° C. for 24 hours to dissolve sericin;

[0047] (3) Transfer the solution obtained in step (2) into a centrifuge tube and centrifuge at 5000 rpm for 10 minutes to remove insoluble substances and obtain a c...