Method for inhibiting Phytophthora capsici by utilizing residue of Chinese herbal medicine for clearing away heat and toxic material

Abstract

A method for inhibiting Phytophthora capsici by utilizing residue of Chinese herbal medicine for clearing away heat and toxic material is provided in order to solve that infection problem of Phytophthora capsici and belongs to the field of soil-borne pathogens control technology. The alcohol extract of Chinese herbal medicine residue with heat-clearing and detoxifying effect is used to inhibit thegrowth of Phytophthora capsici. The Chinese herbal medicine is composed of Aster, Flos Farfarae, tangerine peel, honeysuckle, forsythia suspensa, houttuynia cordata, cortex Lycii, rhizoma Bletillae,Cynanchum atratum Bunge, almond, radix Curcumae, radix Platycodi, radix Cyathulae, Bulbus Fritillariae Cirrhosae and liquorice which are uniformly mixed. The residues are extracted with ethanol and dissolved in sterile water. 8-12 mL of the ethanol extract of residues is poured uniformly into the root of pepper when the pepper is transplanted. Chemical pesticide residues is avoided, the accumulation of chemical pesticides in the soil is reduced, and chemical pesticides are prevented from polluting the soil. The colonization ability and adaptability of biocontrol bacteria in soil are overcome,harm to other organisms is little and the ecological balance is ensured; Chinese herbal medicine residues are used as a kind of medical waste for the study of biological pesticides, so as to achieve the secondary utilization of Chinese herbal medicine residues, saving resources.

Description

technical field

[0001] The invention belongs to the technical field of prevention and control of soil-borne pathogenic bacteria, and in particular relates to a method for inhibiting Phytophthora capsici by using dregs of Chinese herbal medicine for clearing away heat and detoxifying. Background technique

[0002] Phytophthora capsici is a soil-borne pathogen with a wide range of hazards. It mainly infects the stem base of pepper and causes pepper blight. The spread of Phytophthora capsici is very fast, as long as a few plants are infected, a spread center will be formed, thereby expanding the area of ​​infected plants. Pepper blight has a great impact on the yield and quality of pepper, and in severe cases, the yield can be reduced by more than 50%. Phytophthora capsici is a soil-borne pathogen with a wide range of hazards. It has been found in all continents of the world, and its host range continues to expand. At present, it can harm 78 species of plants in 18 families, t...

Examples

Embodiment 1

[0011] Example 1: Preparation of alcohol extract of Chinese herbal medicine dregs with heat-clearing and detoxifying effects: The same dose of Chinese herbal medicine dregs with heat-clearing and detoxifying effects brought back from Taiyuan Central Hospital, the Chinese herbal medicines are specifically: Aster 12 grams, Winter Flower 12 grams , 12 grams of tangerine peel, 20 grams of silver flower, 20 grams of forsythia, 20 grams of Houttuynia cordata, 12 grams of Digupi, 12 grams of bletilla striata, 12 grams of Baiwei, 9 grams of almonds, 12 grams of turmeric, 12 grams of platycodon, Sichuan Achyranthes bidentata 15 grams, Chuan Bei 9 grams, licorice 6 grams.

[0012] The medicine dregs brought back were numbered 1 and 2, drained, and dried in the sun. Weighed 10 g of each medicine residue and put them into conical flasks, and added 100 mL of 80% ethanol to each conical flask , leached in a constant temperature oscillator for 2-3 days, filtered and centrifuged, and the supe...

Embodiment 2

[0013] Example 2: Plate experiments on the inhibition of Phytophthora capsici by the prepared heat-clearing and detoxifying Chinese herbal medicine dregs ethanol extract:

[0014] 1. Preparation of Phytophthora capsici suspension: On the ultra-clean workbench, elute the cells of Phytophthora capsicum on the inclined plane of the expanded cultured Phytophthora capsici test tube, and dilute the concentration of the bacterial suspension to about 10 6 CFU / mL. Store in the refrigerator for later use.

[0015] 2. Bacteriostatic experiment of the prepared alcoholic extract of Chinese herbal medicine dregs on Phytophthora capsici: Use a puncher to punch 4 holes evenly on the medium, and then use a pipette gun to inject the liquid into the holes. The first is 0.2 mL of the alcoholic extract of Chinese herbal medicine dregs, and the control is repeated three times. Then use a pipette gun to inject 0.2 mL of sterile water into the fourth hole as a blank control and label it. All cult...

Embodiment 3

[0018] Embodiment 3: the field control effect of the ethanol extract of Chinese herbal medicine dregs to Phytophthora capsici:

[0019] Field experiments were used to detect the control effect of Chinese herbal medicine dregs on Phytophthora capsicum. Transplant 3 healthy pepper seedlings at the 4-leaf stage. The settings were: ① Control 1: the soil was not inoculated with any microorganisms; ② Control 2: the soil was inoculated with Phytophthora capsici; ③ Treatment experiment: the soil was simultaneously added with the alcohol extract of Chinese herbal medicine No. 1 (concentration: 2 g / mL) and Phytophthora capsici . The inoculum amount of Phytophthora capsici is 500-800 sporangia in 1 g of air-dried soil; when the pepper is planted, 8-12 mL is evenly poured into it from the root. Each treatment had 6 plants, and the experiment was repeated three times. All controls and treatments were surveyed 7 and 14 days after transplanting, and the incidence was counted, and the inci...

technical field

[0001] The invention belongs to the technical field of prevention and control of soil-borne pathogenic bacteria, and in particular relates to a method for inhibiting Phytophthora capsici by using dregs of Chinese herbal medicine for clearing away heat and detoxifying. Background technique

[0002] Phytophthora capsici is a soil-borne pathogen with a wide range of hazards. It mainly infects the stem base of pepper and causes pepper blight. The spread of Phytophthora capsici is very fast, as long as a few plants are infected, a spread center will be formed, thereby expanding the area of ​​infected plants. Pepper blight has a great impact on the yield and quality of pepper, and in severe cases, the yield can be reduced by more than 50%. Phytophthora capsici is a soil-borne pathogen with a wide range of hazards. It has been found in all continents of the world, and its host range continues to expand. At present, it can harm 78 species of plants in 18 families, t...

Examples

Embodiment 1

[0011] Example 1: Preparation of alcohol extract of Chinese herbal medicine dregs with heat-clearing and detoxifying effects: The same dose of Chinese herbal medicine dregs with heat-clearing and detoxifying effects brought back from Taiyuan Central Hospital, the Chinese herbal medicines are specifically: Aster 12 grams, Winter Flower 12 grams , 12 grams of tangerine peel, 20 grams of silver flower, 20 grams of forsythia, 20 grams of Houttuynia cordata, 12 grams of Digupi, 12 grams of bletilla striata, 12 grams of Baiwei, 9 grams of almonds, 12 grams of turmeric, 12 grams of platycodon, Sichuan Achyranthes bidentata 15 grams, Chuan Bei 9 grams, licorice 6 grams.

[0012] The medicine dregs brought back were numbered 1 and 2, drained, and dried in the sun. Weighed 10 g of each medicine residue and put them into conical flasks, and added 100 mL of 80% ethanol to each conical flask , leached in a constant temperature oscillator for 2-3 days, filtered and centrifuged, and the supe...

Embodiment 2

[0013] Example 2: Plate experiments on the inhibition of Phytophthora capsici by the prepared heat-clearing and detoxifying Chinese herbal medicine dregs ethanol extract:

[0014] 1. Preparation of Phytophthora capsici suspension: On the ultra-clean workbench, elute the cells of Phytophthora capsicum on the inclined plane of the expanded cultured Phytophthora capsici test tube, and dilute the concentration of the bacterial suspension to about 10 6 CFU / mL. Store in the refrigerator for later use.

[0015] 2. Bacteriostatic experiment of the prepared alcoholic extract of Chinese herbal medicine dregs on Phytophthora capsici: Use a puncher to punch 4 holes evenly on the medium, and then use a pipette gun to inject the liquid into the holes. The first is 0.2 mL of the alcoholic extract of Chinese herbal medicine dregs, and the control is repeated three times. Then use a pipette gun to inject 0.2 mL of sterile water into the fourth hole as a blank control and label it. All cult...

Embodiment 3

[0018] Embodiment 3: the field control effect of the ethanol extract of Chinese herbal medicine dregs to Phytophthora capsici:

[0019] Field experiments were used to detect the control effect of Chinese herbal medicine dregs on Phytophthora capsicum. Transplant 3 healthy pepper seedlings at the 4-leaf stage. The settings were: ① Control 1: the soil was not inoculated with any microorganisms; ② Control 2: the soil was inoculated with Phytophthora capsici; ③ Treatment experiment: the soil was simultaneously added with the alcohol extract of Chinese herbal medicine No. 1 (concentration: 2 g / mL) and Phytophthora capsici . The inoculum amount of Phytophthora capsici is 500-800 sporangia in 1 g of air-dried soil; when the pepper is planted, 8-12 mL is evenly poured into it from the root. Each treatment had 6 plants, and the experiment was repeated three times. All controls and treatments were surveyed 7 and 14 days after transplanting, and the incidence was counted, and the inci...

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