An instrument device for anhydrous dry cell resuscitation

Abstract

The invention discloses an instrument device for waterless dry resuscitation of cells, comprising a resuscitation chamber, a base and a bottom support type mixing device, wherein the resuscitation chamber is located above the base, the resuscitation chamber comprises a resuscitation chamber body and a resuscitation chamber cover movably connected to the resuscitation chamber body, the resuscitation chamber cover can be covered on the resuscitation chamber body to form a closed resuscitation space, a dual-function rewarming tray is arranged in the resuscitation chamber body, a dual-function rewarming hot cover is arranged in the resuscitation chamber cover, and a power source of the bottom support type kneading mixing device is arranged in the base, and is movably connected to the dual-function rewarming tray through a power bracket. The invention is suitable for waterless dry resuscitation applications of cell cryopreservation bags or cell cryopreservation tubes.

Description

Technical Field

[0001] The invention relates to the field of cell resuscitation, and in particular to an instrument device for anhydrous dry resuscitation of cells. Background Art

[0002] With the development of life science and technology, cell therapy technology is changing with each passing day, mainly including stem cell therapy and immune cell therapy represented by CAR-T cells. The preparation process of cell products involves cell freezing and thawing. When thawing cells, the best operation is to quickly heat the cell freezing bag or cell freezing tube just taken out of the liquid nitrogen tank to 37°C, so that it quickly passes through -5°C to 0°C where cells are most vulnerable, to avoid ice crystals re-crystallizing and causing damage to cells, leading to cell death.

[0003] There are two common types of cell cryopreservation containers: cell cryopreservation tubes and cell cryopreservation bags. The cryopreservation capacity of cell cryopreservation tubes is usually less than 5ml, and cell cryopreservation bags are often used for cell cryopreservation needs exceeding 5ml. Cell cryopreservation bags are more suitable for commercial applications of stem cells and immune cells, such as CAR-T cell therapy. At present, cell cryopreservation bags can be resuscitated by water bath resuscitation or dry resuscitation. The former water bath resuscitation method requires manual operation throughout the process, cannot be standardized, and is prone to cell contamination, and has been gradually phased out. The latter dry resuscitation method currently has limited products, a single resuscitation method, and unsatisfactory resuscitation efficiency, and there is still a lot of room for improvement and development.

[0004] In any heating device, the heat source transfers heat energy to the heated object in the form of convection, radiation and conduction. Heat conduction refers to the heat transfer phenomenon that relies on the thermal motion of microscopic particles such as molecules, atoms and free electrons when there is no relative displacement between the parts of an object or when different objects are in direct contact. Convection is the phenomenon of transferring heat from one place to another by relying on the movement of fluid. Whether it is heat conduction or convection, heat must be transferred through direct contact between hot and cold objects or by relying on conventional substances as a medium. However, the principle of thermal radiation is completely different. It relies on the visible and invisible rays emitted by the surface of an object to transfer heat. Radiant heating has a fast transfer speed and does not pass through any medium, which greatly reduces the loss in the process of heat transfer, thereby improving the utilization rate of heat energy.

[0005] In far-infrared heating technology, radiation heating is the main method. When far-infrared rays irradiate the object to be heated, part of the rays are reflected back and part of them are penetrated. When the wavelength of the emitted far-infrared rays is consistent with the absorption wavelength of the heated object, the heated object absorbs a large amount of far-infrared rays, causing the molecules and atoms inside the object to undergo " Resonance "- Produces strong vibration and rotation, and the vibration and rotation increase the temperature of the object, thereby achieving the purpose of heating.

[0006] Far infrared is a type of light that cannot be seen by the naked eye. It is different from general infrared (near infrared) which only has a thermal effect on the human body. It efficiently converts the surrounding heat energy into far infrared radiation through specific infrared materials. Far infrared with a wavelength range of 3 to 10 μm can be absorbed by water molecules and organic molecules in organisms, so it can transfer energy into the body and induce a series of physiological effects that are beneficial to human health. Using this mechanism, far infrared can be widely used in medical care, which is called "far infrared therapy."

[0007] The energy emission mode of carbon crystal electric heating plate or carbon fiber electric heating plate is mainly far infrared radiation. After being powered on, carbon crystal electric heating plate or carbon fiber electric heating plate will radiate infrared energy that can heat the material. The infrared wavelength of the radiation is 2.3-14μm. The far infrared spectrum of this wavelength is called "light of life", accounting for more than 80% of the total wavelength. It can be absorbed by water molecules to produce resonant friction heat effect, realizing the effect of quickly increasing the temperature of the heated material. In particular, it can effectively activate human tissue cells, promote blood circulation, accelerate new metabolism, enhance immunity and other health effects.

[0008] At present, there is no application of water-free dry resuscitation of cell cryopreservation bags or cell cryopreservation tubes by simultaneously utilizing warm plate conduction heating and far infrared radiation heating. Summary of the invention

[0009] The object of the present invention is to provide an instrument device for anhydrous dry resuscitation of cells to solve one or more of the above-mentioned problems in the prior art.

[0010] An instrument device for anhydrous dry cell resuscitation according to the present invention comprises a resuscitation chamber, a base and a bottom support type mixing device, wherein the resuscitation chamber is located above the base;

[0011] The resuscitation cabin comprises a resuscitation cabin body and a resuscitation cabin cover movably connected to the resuscitation cabin body, the resuscitation cabin cover can cover the resuscitation cabin body to form a closed resuscitation space, a dual-function rewarming tray is arranged in the resuscitation cabin body, and a dual-function rewarming heat cover is arranged in the resuscitation cabin cover;

[0012] The bottom support type mixing device is arranged in the base and connected with the dual-function rewarming tray in the resuscitation cabin.

[0013] The present invention provides an instrument device for anhydrous dry resuscitation of cells, which can be used for anhydrous dry resuscitation of cell cryopreservation bags and anhydrous dry resuscitation of high-throughput cell cryopreservation tubes; at the same time, the two rewarming methods of contact conduction heating and far-infrared radiation heating are integrated together, and the rewarming efficiency of cell cryopreservation bags or cell cryopreservation tubes is higher.

[0014] In some embodiments, the dual-work rewarming tray is removable and replaceable, a buffer device and a pressure sensor are provided between the dual-work rewarming thermal cover and the resuscitation cabin cover, the dual-work rewarming thermal cover and the resuscitation cabin cover are movably connected through the buffer device and the pressure sensor, the dual-work rewarming thermal cover is removable and replaceable, and an exhaust fan is also provided in the resuscitation cabin cover.

[0015] In some embodiments, air is sucked in from the vents on the bottom, side walls and rear wall of the base, rises along the gap inside the resuscitation chamber cavity, and is exhausted by the exhaust fan. The base is provided with a numerical control device, a communication device and a touch screen.

[0016] In some embodiments, the dual-function rewarming tray includes a tray frame and a first heating body, the tray frame is made of metal, the first heating body is arranged in the tray frame, the first heating body is an electric heating plate having both conduction heating and far-infrared radiation heating functions, the first heating body is fixedly connected to the tray frame, the tray frame is provided with a first temperature control temperature sensor, the number of the first temperature control temperature sensors is 1 to 6, the first heating body is provided with a through hole, the through hole is provided with a first rewarming monitoring temperature sensor, the number of the first rewarming monitoring temperature sensors is 1 to 6;

[0017] The operating temperature range of the dual-function thawing tray is 10° C. to 70° C., and a guide line for placing a cell freezing bag or a cell freezing tube is drawn on the dual-function thawing tray.

[0018] In some embodiments, the temperature control temperature sensor and the retemperature monitoring temperature sensor include contact temperature sensors and non-contact temperature sensors. The contact temperature sensor includes a thermistor sensor, a thermocouple sensor, a thermocouple string sensor, a digital sensor, a platinum sensor, etc. The non-contact temperature sensor includes a thermopile array non-contact temperature sensor, an infrared temperature probe temperature sensor, an infrared thermal imager temperature sensor, etc.

[0019] In some embodiments, the dual-function rewarming heat cover includes a heat cover frame and a second heating body, the heat cover frame is made of metal, the second heating body is located in the heat cover frame, the second heating body is an electric heating plate having both conduction heating and far-infrared radiation heating functions, the second heating body is fixedly connected to the heat cover frame, the heat cover frame is provided with a second temperature control temperature sensor, the number of the second temperature control temperature sensors is 1 to 6, the second heating body is provided with a through hole, a second rewarming monitoring temperature sensor is provided in the through hole, the number of the second rewarming monitoring temperature sensors is 1 to 6, the operating temperature range of the dual-function rewarming heat cover is 10°C to 70°C, and the dual-function rewarming heat cover is painted with a guide line for the placement of cell freezing bags or cell freezing tubes.

[0020] In some embodiments, the buffer device is a hydraulic buffer, a spring buffer or a damping buffer, and the damping buffer includes an air damping buffer, a gear damping buffer, a rack damping buffer, and a cylindrical damping buffer.

[0021] In some embodiments, the pressure sensor is a piezoresistive pressure sensor, a ceramic pressure sensor, a diffused silicon pressure sensor, a sapphire pressure sensor, or a piezoelectric pressure sensor.

[0022] In some embodiments, the bottom support type mixing device includes a power source and a power support, the power source is fixedly connected to the bottom of the base by bolts, the power source includes a servo motor and a motor fixing bracket, the motor shaft end of the servo motor is provided with an eccentric wheel, the number of the servo motors is 2 or 4, the number of the power supports is 4, the number of the eccentric wheels is 4, the size specifications of the 4 eccentric wheels are the same, and the initial eccentric distances of the 4 eccentric wheels in the vertical direction can be adjusted separately during installation, the power support movably connects the eccentric wheel and the dual-function rewarming tray through a ball and socket structure, the operation of the servo motor is regulated by the numerical control device, and the bottom support type mixing device can drive the dual-function rewarming tray to realize the bottom support type kneading and oscillating mixing movement.

[0023] In some embodiments, the bottom support mixing device can also be an electric fast hydraulic push rod, an electric hydraulic push rod, an electric push rod, etc. The number of the electric fast hydraulic push rod, or the electric hydraulic push rod, or the electric push rod, etc. is , which are respectively movably connected to the double-function rewarming tray, and the operation of the electric fast hydraulic push rod, or the electric hydraulic push rod, or the electric push rod, etc. is regulated by the numerical control device.

[0024] In some embodiments, the communication device can be connected to the Internet or a local area network through wired or wireless networking to perform data exchange, data storage, and data query.

[0025] A working method of an instrument device for anhydrous dry resuscitation of cells, comprising the following steps:

[0026] (1) Start the instrument, set the required temperature for recovery, select the recovery working mode, turn on the preheating function, and the dual-function recovery tray and the dual-function recovery cover begin to preheat. The temperature of the dual-function recovery tray and the dual-function recovery cover begins to rise and emit far infrared rays. After the preheating is completed, place the cell cryopreservation bag or cell cryopreservation tube on the dual-function recovery tray, cover and fix the recovery cabin cover. Under the action of the buffer device between the dual-function recovery cover and the recovery cabin cover, the cell cryopreservation bag or cell cryopreservation tube is clamped by the dual-function recovery tray and the dual-function recovery cover. Cell recovery begins, and the dual-function recovery tray and the dual-function recovery cover move toward the cell. The cryopreservation bag or cryopreservation tube transfers heat through conduction and radiation at the same time, and the bottom support mixing device starts to work. Under the control of the numerical control device, the four eccentric wheels have different starting working angles and running speeds, driving the dual-function rewarming tray, the cryopreservation bag or cryopreservation tube, and the dual-function rewarming hot cover to perform bottom support kneading and oscillating mixing movements. The force applied by the mixing movement can be detected by the pressure sensor between the dual-function rewarming hot cover and the recovery cabin cover, and transmitted to the numerical control device, which intelligently feedbacks and adjusts the operation of the power source to prevent excessive force from damaging the cryopreservation bag or instrument.

[0027] (2) The temperature of the dual-function rewarming tray and the dual-function rewarming hot cover is monitored at any time by a temperature control temperature sensor, and the detection data is transmitted to the numerical control device, which controls the heating process of the dual-function rewarming tray and the dual-function rewarming hot cover to intelligently maintain the set temperature;

[0028] (3) The rewarming monitoring temperature sensor detects whether the current temperature of the cell cryopreservation bag or cell cryopreservation tube matches the set rewarming target temperature. The matching stability judgment time is no more than 5 seconds. If it is lower than the rewarming target temperature, the heating rewarming state is turned on and maintained. If it is higher than the rewarming target temperature, the heating is stopped immediately.

[0029] (4) When in heating mode, the dual-function heating tray and the dual-function rewarming heat cover are in the heating state, the temperature of the tray and the heat cover rises, and far infrared rays are emitted, and the heat is transferred to the cell cryopreservation bag or cell cryopreservation tube through two heat conduction modes, conduction and radiation, for rewarming;

[0030] (5) When the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0°C to 8°C, it can be judged that the ice in the cell cryopreservation bag or the cell cryopreservation tube is completely melted, and the heating mode is turned off, the dual-function rewarming tray and the dual-function rewarming thermal cover stop working, and the exhaust fan is started at the same time, and air is sucked in from the vents on the bottom, side wall and rear wall of the base, rises along the gap inside the resuscitation chamber, and is finally discharged from the exhaust fan, taking away the heat of the dual-function rewarming tray and the dual-function rewarming thermal cover, and the bottom support mixing device continues to perform the bottom support kneading and oscillating mixing movement;

[0031] (6) When the cell resuscitation work of the cell cryopreservation bag or cell cryopreservation tube is completed, the operator is reminded to take away the resuscitated cell cryopreservation bag or cell cryopreservation tube in time through sound and light signals such as flashing indicator lights and sounding speakers.

[0032] The present invention has the following advantages and beneficial effects:

[0033] 1. The present invention can be used for anhydrous dry resuscitation of cell cryopreservation bags and anhydrous dry resuscitation of high-throughput cell cryopreservation tubes;

[0034] 2. The present invention uses an electric heating plate component that has both flat heating and far-infrared radiation heating functions, and integrates the two rewarming methods of contact conduction heating and far-infrared radiation heating, so that the rewarming efficiency of cell cryopreservation bags or cell cryopreservation tubes is higher;

[0035] 3. The dual-function rewarming tray is placed in the resuscitation chamber and can perform bottom-supporting oscillating mixing motion. Compared with the external mixing working mode, the built-in mixing working mode does not require a sealed working chamber to be set on the dual-function rewarming tray. The dual-function rewarming tray is light in weight, simpler in structure, and easy to implement technically.

[0036] 4. The number of servo motors of the bottom support type mixing device of the four-point support type double-function reheating tray is 4. Four connecting rods are used to flexibly connect the four eccentric wheels with the double-function reheating tray through four supporting points. The operation of the servo motor is regulated by the numerical control device. By changing the speed of the servo motor and the starting operating angle of the eccentric wheel, the four-point support type double-function reheating tray can realize complex and diverse bottom support type oscillation mixing movements. Its mixing effect is significantly stronger than the two-point support type seesaw type mixing effect with only one servo motor;

[0037] 5. The bottom support type mixing device cooperates with the buffer device in the recovery hatch, so that the cell cryopreservation bag or cell cryopreservation tube can be clamped by the dual-function rewarming tray and the dual-function rewarming hot cover, which is beneficial to heat conduction. The bottom support type mixing device can apply a squeezing action to the cell cryopreservation bag, which is very beneficial to the flow and mixing of the liquid in the cell cryopreservation bag. The relatively high temperature liquid heated on the periphery and the relatively low temperature liquid near the ice cube inside can be mixed in time. On the one hand, it protects the cells from being inactivated by high temperature, and on the other hand, it is convenient to quickly and evenly bring heat to the ice cube to achieve rapid thawing and melting;

[0038] 6. In addition to the buffer device, there is also a pressure sensor between the recovery hatch and the dual-function rewarming heat cover. The force applied by the power source of the bottom-supporting mixing device can be detected by the pressure sensor between the dual-function rewarming heat cover and the recovery hatch, and transmitted to the CNC device, and the operation of the power source is adjusted by intelligent feedback to prevent excessive force from causing damage to the cell cryopreservation bag or instrument;

[0039] 7. An exhaust fan is also provided inside the resuscitation chamber cover. Air is sucked in from the vents at the bottom, side walls and rear wall of the base, rises along the gap inside the resuscitation chamber, and is finally discharged from the exhaust fan, taking away the heat from the dual-function rewarming tray and the dual-function rewarming thermal cover. This helps to reduce the impact of the residual heat of the dual-function rewarming tray and the dual-function rewarming thermal cover on the cell cryopreservation bags or cell cryopreservation tubes after the rewarming is completed. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 A schematic structural diagram of an instrument device for anhydrous dry cell resuscitation according to an embodiment of the present invention;

[0041] Figure 2 An axonometric diagram of a dual-function rewarming tray, a dual-function rewarming hot cover, a buffer device, and a bottom support type mixing device of an instrument device for anhydrous dry resuscitation of cells according to one embodiment of the present invention;

[0042] Figure 3 A top view of an instrument device for anhydrous dry cell resuscitation according to an embodiment of the present invention;

[0043] Figure 4 A right side view of a dual-function rewarming tray, a dual-function rewarming thermal cover, and a buffer device of an instrument device for anhydrous dry resuscitation of cells according to an embodiment of the present invention;

[0044] Figure 5 The present invention is an axonometric bottom view of a dual-function rewarming tray, a dual-function rewarming heat cover, a buffer device and a bottom support type mixing device of an instrument device for anhydrous dry resuscitation of cells according to an embodiment of the present invention. DETAILED DESCRIPTION

[0045] The present invention will be further described in detail below in conjunction with the accompanying drawings.

[0046] Embodiment 1:

[0047] like Figure 1 As shown in the figure, an instrument device for cell anhydrous dry resuscitation includes a resuscitation chamber 1, a base 2 and a bottom support type mixing device 3, the resuscitation chamber 1 is installed above the base 2, the resuscitation chamber 1 includes a resuscitation chamber body 11 and a resuscitation chamber cover 12 movably connected to the resuscitation chamber body 11, the resuscitation chamber cover 12 can be covered on the resuscitation chamber body 11 to form a closed resuscitation space, a dual-function rewarming tray 111 is provided in the resuscitation chamber body 11, a dual-function rewarming heat cover 121 is provided in the resuscitation chamber cover 12, the dual-function rewarming tray 111 and the dual-function rewarming heat cover 121 can be covered, and cell freezing bags or cell freezing tubes can be placed between the dual-function rewarming tray 111 and the dual-function rewarming heat cover 121 for resuscitation operations.

[0048] The bottom-supporting mixing device 3 is a bottom-supporting massaging and mixing device, which is installed in the base 2 and connected to the dual-function rewarming tray 111 in the resuscitation chamber 11. In this embodiment, the resuscitation chamber 1 is fixed, and the bottom-supporting massaging and mixing device drives the dual-function rewarming tray 111 to perform bottom-supporting massaging and kneading oscillation mixing movement.

[0049] The present invention provides an instrument device for anhydrous dry resuscitation of cells, which can be used for anhydrous dry resuscitation of cell cryopreservation bags and anhydrous dry resuscitation of high-throughput cell cryopreservation tubes; at the same time, the two rewarming methods of contact conduction heating and far-infrared radiation heating are integrated together, and the rewarming efficiency of cell cryopreservation bags or cell cryopreservation tubes is higher.

[0050] Among the above, Figures 2 to 4 As shown in , the dual-function rewarming tray 111 is detachable and replaceable, and a buffer device 122 and a pressure sensor 124 are installed between the dual-function rewarming heat cover 121 and the resuscitation cabin cover 12. The dual-function rewarming heat cover 121 and the resuscitation cabin cover 12 are movably connected through the buffer device 122 and the pressure sensor 124. The pressure sensor 124 is installed in the middle or beside the buffer device 122. The buffer device 122 is a spring in this embodiment, one end of which is fixedly installed at the four corners of the dual-function rewarming heat cover 121 by bolts, and the other end is fixedly installed on the resuscitation cabin cover 12 by bolts. The dual-function rewarming heat cover 121 is also detachable and replaceable, and an exhaust fan 123 is also provided in the resuscitation cabin cover 12. The dual-function rewarming tray 111 and the dual-function rewarming heat cover 121 simultaneously integrate the two rewarming methods of contact conduction heating and far-infrared radiation heating, and the rewarming efficiency is higher.

[0051] Air is sucked in from the vents on the bottom, side walls and rear wall of the base 2, rises along the gap inside the cavity of the resuscitation cabin 1, and is exhausted by the exhaust fan 123. The base 2 is provided with a numerical control device 21, a communication device 22 and a touch screen 23.

[0052] The dual-function rewarming tray 111 includes a tray frame 1111 and a first heating body 1112. The tray frame 1111 is made of metal. The first heating body 1112 is installed in the tray frame 1111. The first heating body 1112 is an electric heating plate with both conduction heating and far-infrared radiation heating functions. The electric heating plate includes a carbon crystal heating plate, a carbon fiber heating plate, etc. The first heating body 1112 is fixedly connected to the tray frame 1111 by bolts. The tray frame 1111 is equipped with a first temperature control temperature sensor, which can monitor the temperature of the first heating body 1112 in real time. The number of the first temperature control temperature sensors is 1 to 6. A through hole is opened on the first heating body 1112, and a first rewarming monitoring temperature sensor 1114 is arranged in the through hole, which can monitor the temperature of the cell freezing bag in real time. The number of the first rewarming monitoring temperature sensors 1114 is 1 to 6, and the number in this embodiment is 2.

[0053] The operating temperature range of the dual-function thawing tray 111 is 10°C to 70°C, which can be set through the touch screen 23. The dual-function thawing tray 111 is painted with a placement guide line for cell cryopreservation bags or cell cryopreservation tubes. The dual-function thawing tray 111 can be a flat tray, which is suitable for dry resuscitation of cell cryopreservation bags.

[0054] The dual-function rewarming tray 111 may also be provided with a plurality of cryotube slots, which are rectangular in shape and semicircular in cross section, and are suitable for dry resuscitation of high-throughput cell cryotubes. The dual-function rewarming tray 111 is electrically connected to the numerical control device 21 via a connector device.

[0055] The temperature control temperature sensor 1113 and the re-temperature monitoring temperature sensor 1114 include contact temperature sensors and non-contact temperature sensors. The contact temperature sensors include thermistor sensors, thermocouple sensors, thermocouple string sensors, digital sensors, platinum sensors, etc. The non-contact temperature sensors include thermopile array non-contact temperature sensors, infrared temperature probe temperature sensors, infrared thermal imager temperature sensors, etc.

[0056] The dual-function temperature-recovering heat cover 121 includes a heat cover frame 1211 and a second heating body 1212. The heat cover frame 1211 is made of metal. The second heating body 1212 is located in the heat cover frame 1211. The second heating body 1212 is an electric heating plate with both conduction heating and far-infrared radiation heating functions. The electric heating plate includes a carbon crystal electric heating plate, a carbon fiber electric heating plate, etc. The second heating body 1212 is fixedly connected to the heat cover frame 1211. The heat cover frame 1211 is provided with a second temperature control temperature sensor 1213, which can monitor the temperature of the second heating body 1212 in real time. The temperature of the second heating body 1212, the number of the second temperature control temperature sensors 1213 is 1 to 6, the second heating body 1212 is provided with a through hole, and the second rewarming monitoring temperature sensor 1214 is provided in the through hole, which can monitor the temperature of the cell freezing bag in real time, the number of the second rewarming monitoring temperature sensors 1214 is 1 to 6, the operating temperature range of the dual-function rewarming thermal cover 121 is 10℃~70℃, which can be set through the touch screen 23, and the dual-function rewarming thermal cover 121 is painted with a placement guide line for cell freezing bags or cell freezing tubes.

[0057] The dual-function rewarming thermal cover 121 can be in the shape of a flat plate, which is suitable for dry resuscitation of cell cryopreservation bags. The dual-function rewarming thermal cover 121 can also be provided with a plurality of cryopreservation tube slots, which are in the shape of long rectangles and semicircular in cross-section, and are suitable for dry resuscitation of high-throughput cell cryopreservation tubes. The cryopreservation tube slots of the dual-function rewarming thermal cover 121 are arranged correspondingly to the cryopreservation tube slots of the dual-function rewarming tray 111, and the dual-function rewarming thermal cover 121 is electrically connected to the numerical control device 21 via a connector device.

[0058] The buffer device 122 is a hydraulic buffer, a spring buffer or a damping buffer. The damping buffer includes an air damping buffer, a gear damping buffer, a rack damping buffer, and a cylindrical damping buffer.

[0059] The pressure sensor 124 is a piezoresistive pressure sensor, a ceramic pressure sensor, a diffused silicon pressure sensor, a sapphire pressure sensor, or a piezoelectric pressure sensor.

[0060] like Figure 5 As shown in the figure, the bottom-supporting massaging and mixing device includes a power source 31 and a power bracket 32. The power source 31 is fixedly connected to the bottom of the base 2. The power source 31 includes a servo motor 311 and a motor bracket 312. The motor shaft end of the servo motor 311 is provided with an eccentric wheel 313. The number of servo motors 311 is 2 or 4, the number of power brackets 32 is 4, the number of eccentric wheels 313 is 4, the size specifications of the four eccentric wheels 313 are the same, and the initial eccentric distances of the four eccentric wheels 313 in the vertical direction can be adjusted respectively during installation. The power bracket 32 ​​movably connects the eccentric wheel 313 with the dual-function rewarming tray 111 through a ball-and-socket structure. The operation of the servo motor 311 is regulated by the numerical control device 21. The bottom-supporting massaging and mixing device 3 can drive the dual-function rewarming tray 111 to realize the bottom-supporting massaging and oscillating mixing movement.

[0061] In the above, the bottom-supporting kneading and mixing device can also be an electric fast hydraulic push rod, an electric hydraulic push rod, an electric push rod, etc. The number of the electric fast hydraulic push rod or the electric hydraulic push rod or the electric push rod is 4, which are respectively movably connected to the double-function rewarming tray 111, and the operation of the electric fast hydraulic push rod or the electric hydraulic push rod or the electric push rod is regulated by the numerical control device 21.

[0062] The communication device 22 can be connected to the Internet or a local area network through wired or wireless networking to exchange data, store data, and query data.

[0063] Embodiment 1 is an instrument device for anhydrous dry resuscitation of cells, wherein the resuscitation chamber and the base are integrally fixedly connected, the dual-function rewarming tray is placed in the resuscitation chamber, the bottom-supporting massaging and mixing device can drive the dual-function rewarming tray to perform bottom-supporting massaging and oscillating mixing movements, and the dual-function rewarming tray moves in the resuscitation chamber, which is a built-in mixing working mode.

[0064] A working method of an instrument device for anhydrous dry cell resuscitation according to Example 1, comprising the following steps:

[0065] (1) Start the instrument, set the required temperature for recovery, select the recovery working mode, turn on the preheating function, and the dual-function recovery tray and the dual-function recovery cover begin to preheat. The temperature of the dual-function recovery tray and the dual-function recovery cover begins to rise and emit far infrared rays. After the preheating is completed, place the cell cryopreservation bag or cell cryopreservation tube on the dual-function recovery tray, cover and fix the recovery cabin cover. Under the action of the buffer device between the dual-function recovery cover and the recovery cabin cover, the cell cryopreservation bag or cell cryopreservation tube is clamped by the dual-function recovery tray and the dual-function recovery cover. Cell recovery begins, and the dual-function recovery tray and the dual-function recovery cover are pressed against the cell. The cryopreservation bag or cell cryopreservation tube transfers heat through conduction and radiation at the same time, and the bottom-supporting kneading and mixing device starts to work. Under the control of the numerical control device, the four eccentric wheels have different starting working angles and running speeds, driving the dual-function rewarming tray, cell cryopreservation bag or cell cryopreservation tube, and dual-function rewarming hot cover to perform bottom-supporting kneading and oscillating mixing movements. The force applied by the mixing movement can be detected by the pressure sensor between the dual-function rewarming hot cover and the recovery cabin cover, and transmitted to the numerical control device, which intelligently feedbacks and adjusts the operation of the power source to prevent excessive force from damaging the cell cryopreservation bag or instrument.

[0066] (2) The temperature of the dual-function rewarming tray and the dual-function rewarming hot cover is monitored at any time by a temperature control temperature sensor, and the detection data is transmitted to the numerical control device, which controls the heating process of the dual-function rewarming tray and the dual-function rewarming hot cover to intelligently maintain the set temperature;

[0067] (3) The rewarming monitoring temperature sensor detects whether the current temperature of the cell cryopreservation bag or cell cryopreservation tube matches the set rewarming target temperature. The matching stability judgment time is no more than 5 seconds. If it is lower than the rewarming target temperature, the heating rewarming state is turned on and maintained. If it is higher than the rewarming target temperature, the heating is stopped immediately.

[0068] (4) When in heating mode, the dual-function heating tray and the dual-function rewarming heat cover are in the heating state, the temperature of the tray and the heat cover rises, and far infrared rays are emitted, and the heat is transferred to the cell cryopreservation bag or cell cryopreservation tube through two heat conduction modes, conduction and radiation, for rewarming;

[0069] (5) When the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0°C to 8°C, it can be judged that the ice in the cell cryopreservation bag or the cell cryopreservation tube is completely melted, and the heating mode is turned off, the dual-function rewarming tray and the dual-function rewarming thermal cover both stop working, and the exhaust fan is started at the same time, and air is sucked in from the vents on the bottom, side wall and rear wall of the base, rises along the gap inside the resuscitation chamber, and is finally discharged from the exhaust fan, taking away the heat of the dual-function rewarming tray and the dual-function rewarming thermal cover, and the bottom support type massaging and kneading mixing device continues to perform the bottom support type massaging and kneading oscillation mixing motion;

[0070] (6) When the cell resuscitation work of the cell cryopreservation bag or cell cryopreservation tube is completed, the operator is reminded to take away the resuscitated cell cryopreservation bag or cell cryopreservation tube in time through sound and light signals such as flashing indicator lights and sounding speakers.

[0071] Embodiment 2:

[0072] An instrument device for anhydrous dry resuscitation of cells includes a resuscitation chamber 1, a base 2 and a bottom-supporting mixing device 3. The bottom-supporting mixing device 3 is a bottom-supporting three-dimensional mixing device. The resuscitation chamber 1 and the base 2 are designed as separate parts. The bottom-supporting three-dimensional mixing device is installed in the base 2. The resuscitation chamber 1 and the base 2 are movably connected through a power bracket 32 ​​of the bottom-supporting three-dimensional mixing device. The bottom-supporting three-dimensional mixing device supports the entire resuscitation chamber 1 through four power brackets 32.

[0073] The resuscitation cabin 1 includes a resuscitation cabin body 11 and a resuscitation cabin cover 12. The resuscitation cabin body 11 is movably connected to the resuscitation cabin cover 12. The resuscitation cabin cover 12 can cover the resuscitation cabin body 11 to form an enclosed resuscitation space. A dual-function rewarming tray 111 is provided in the resuscitation cabin body 11. The dual-function rewarming tray 111 is removable and replaceable. A dual-function rewarming thermal cover 121 is provided in the resuscitation cabin cover 12. The dual-function rewarming thermal cover 121 is movably connected to the resuscitation cabin cover 12. A buffer device 122 and a pressure sensing device 124 are provided between the dual-function rewarming thermal cover 121 and the resuscitation cabin cover 12. The dual-function rewarming thermal cover 121 is removable and replaceable.

[0074] The base 2 is also provided with a numerical control device, a communication device and a touch screen.

[0075] The dual-function reheating tray 111 includes a tray frame 1111 and a first heating body 1112. The tray frame 1111 is made of metal. The first heating body 1112 is located in the tray frame 1111. The first heating body 1112 is an electric heating plate with both conduction heating and far-infrared radiation heating functions. The electric heating plate includes a carbon crystal electric heating plate, a carbon fiber electric heating plate, etc. The first heating body 1112 is fixedly connected to the tray frame 1111. The tray frame 1111 is equipped with a temperature control temperature sensor, which can monitor the temperature of the first heating body in real time. The temperature of 1112, the number of temperature control temperature sensors is 1 to 6, preferably 2, a through hole is provided at the center of the first heating body 1112, a rewarming monitoring temperature sensor 1114 is provided in the through hole, which can monitor the temperature of the cell freezing bag in real time, the number of rewarming monitoring temperature sensors 1114 is 1 to 6, preferably 2, the operating temperature range of the dual-function rewarming tray 111 is 10°C to 70°C, which can be set through the touch screen, and the dual-function rewarming tray 111 is painted with a guide line for placing cell freezing bags or cell freezing tubes.

[0076] The dual-function rewarming tray 111 can be a flat tray, which is suitable for dry resuscitation of cell cryopreservation bags. The dual-function rewarming tray 111 can also be provided with multiple cryopreservation tube slots, which are rectangular in shape and semicircular in cross-section, and are suitable for dry resuscitation of high-throughput cell cryopreservation tubes. The dual-function rewarming tray 111 is electrically connected to the resuscitation chamber 11 through a connector device.

[0077] Temperature control temperature sensors and retemperature monitoring temperature sensors include contact temperature sensors and non-contact temperature sensors. Contact temperature sensors include thermistor sensors, thermocouple sensors, thermocouple string sensors, digital sensors and platinum sensors, etc. Non-contact temperature sensors include thermopile array non-contact temperature sensors, infrared temperature probe temperature sensors, infrared thermal imager temperature sensors, etc.

[0078] The dual-function rewarming heat cover 112 includes a heat cover frame 1121 and a second heating body 1122. The heat cover frame 1121 is made of metal. The second heating body 1122 is located in the heat cover frame 1121. The second heating body 1122 is an electric heating plate with both conduction heating and far-infrared radiation heating functions. The electric heating plate includes a carbon crystal heating plate, a carbon fiber heating plate, etc. The second heating body 1122 is fixedly connected to the heat cover frame 1121. The heat cover frame 1121 is provided with a temperature control temperature sensor 1123, which can monitor the temperature of the second heating body 1122 in real time. The number of the temperature control temperature sensors 1123 is 1 to 6, preferably 2. The operating temperature range of the dual-function rewarming heat cover 112 is 10°C to 70°C, which can be set through a touch screen. The dual-function rewarming heat cover 112 is painted with a guide line for placing cell freezing bags or cell freezing tubes.

[0079] The dual-function rewarming thermal cover 112 can be in the shape of a flat plate, which is suitable for dry resuscitation of cell cryopreservation bags. The dual-function rewarming thermal cover 112 can also be provided with a plurality of cryopreservation tube slots, which are in the shape of an elongated rectangle and a semicircular cross-section, and are suitable for dry resuscitation of high-throughput cell cryopreservation tubes. The cryopreservation tube slots of the dual-function rewarming thermal cover 112 correspond one-to-one to the cryopreservation tube slots of the dual-function rewarming tray 111, and the dual-function rewarming thermal cover 112 is electrically connected to the resuscitation chamber 11 through a connector device.

[0080] The buffer device 122 includes a hydraulic buffer, a spring buffer, and a damping buffer. The damping buffer includes an air damping buffer, a gear damping buffer, a rack damping buffer, and a cylindrical damping buffer.

[0081] The pressure sensor includes piezoresistive pressure sensor, ceramic pressure sensor, diffused silicon pressure sensor, sapphire pressure sensor and piezoelectric pressure sensor.

[0082] The bottom-supporting three-dimensional mixing device includes a power source 31 and a power bracket 32. The power source 31 is arranged in the base 2 and fixedly connected to the bottom of the base 2. The power source 31 includes a servo motor 311, a motor fixing bracket 312 and an eccentric wheel 313. An eccentric wheel 313 is provided at one end or both ends of the servo motor 311. The number of servo motors 311 is 2 or 4, the number of power brackets 32 is 4, and the number of eccentric wheels 313 is 4. The power bracket 32 ​​movably connects the eccentric wheel 313 to the recovery cabin 1 through 4 ball-and-socket connection points. The operation of the servo motor 311 is regulated by a numerical control device. Under the regulation of the numerical control device, the starting working angles and running speeds of the four eccentric wheels 313 are different. The bottom-supporting three-dimensional mixing device can drive the recovery cabin 1 as a whole to realize complex three-dimensional bumpy and oscillating mixing motion.

[0083] The bottom-supporting three-dimensional mixing device can also be an electric fast hydraulic push rod, an electric hydraulic push rod, an electric push rod, etc. There are 4 electric fast hydraulic push rods, electric hydraulic push rods, electric push rods, etc., which are movably connected to the resuscitation cabin through 4 connection points respectively. The operation of the electric fast hydraulic push rod, electric hydraulic push rod, electric push rod, etc. is regulated by the numerical control device, and the operation speed and height are different. The bottom-supporting three-dimensional mixing device can drive the resuscitation cabin as a whole to realize complex three-dimensional bumpy and oscillating mixing movement.

[0084] The communication device can be connected to the Internet or a local area network via wired or wireless networking to exchange data, store data, and query data.

[0085] In Example 2, an instrument device for anhydrous dry resuscitation of cells is provided, wherein the resuscitation chamber and the base are separately arranged, the main body of the bottom-supporting three-dimensional mixing device is arranged in the base, the resuscitation chamber and the base are movably connected through the power bracket of the bottom-supporting three-dimensional mixing device, the bottom-supporting three-dimensional mixing device can drive the resuscitation chamber as a whole to perform complex three-dimensional bumpy oscillating mixing movement, and the movement of the resuscitation chamber drives the movement of the dual-function rewarming tray inside it, which is an external mixing working mode.

[0086] A working method of an instrument device for anhydrous dry cell resuscitation according to Example 2, comprising the following steps:

[0087] (1) Start the instrument, select the resuscitation mode, turn on the preheating function, the dual-function rewarming tray and the dual-function rewarming hot cover start to preheat, the temperature of the dual-function rewarming tray and the dual-function rewarming hot cover starts to rise, and emits far infrared rays. After the preheating is completed, the cell cryopreservation bag or cell cryopreservation tube is placed on the dual-function rewarming tray, and the resuscitation cabin cover is covered and fixed. Cell resuscitation begins, and the dual-function rewarming tray and the dual-function rewarming hot cover transfer heat to the cell cryopreservation bag or cell cryopreservation tube by conduction and radiation at the same time. The bottom support three-dimensional mixing device starts to work, driving the entire resuscitation cabin to perform a complex three-dimensional bumpy oscillation mixing motion;

[0088] (2) The temperature of the dual-function rewarming tray and the dual-function rewarming hot cover is monitored at any time by a temperature control temperature sensor, and the detection data is transmitted to the numerical control device, which controls the heating process of the dual-function rewarming tray and the dual-function rewarming hot cover to intelligently maintain the set temperature;

[0089] (3) The rewarming monitoring temperature sensor instantly monitors whether the current temperature of the cell cryopreservation bag or cell cryopreservation tube matches the set rewarming target temperature. The matching stability judgment time is no more than 5 seconds. If it is lower than the rewarming target temperature, the heating rewarming mode is turned on and maintained. If it is higher than the rewarming target temperature, the heating is stopped immediately.

[0090] (4) When in heating mode, the dual-function heating tray and the dual-function rewarming heat cover are in the heating state, the temperature of the tray and the heat cover rises, and far infrared rays are emitted, and the heat is transferred to the cell cryopreservation bag or cell cryopreservation tube by conduction and radiation for rewarming;

[0091] (5) When the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0°C to 8°C, it can be judged that the ice in the cell cryopreservation bag or cell cryopreservation tube has completely melted, and the heating mode is turned off, the dual-function rewarming tray and the dual-function rewarming hot cover both stop working, and the cell resuscitation work of the cell cryopreservation bag or cell cryopreservation tube is completed. The resuscitation cabin cover automatically opens, and the operator is reminded to take away the resuscitated cell cryopreservation bag or cell cryopreservation tube in time through sound and light signals such as flashing indicator lights and sounding speakers.

[0092] The above is only a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several similar modifications and improvements without departing from the creative concept of the present invention, and these are also considered to be within the scope of invention protection.

Claims

1. An apparatus for anhydrous dry cell resuscitation, characterized in that: The invention comprises a resuscitation chamber (1), a base (2) and a bottom support type mixing device (3), wherein the resuscitation chamber (1) is located above the base (2), the resuscitation chamber (1) comprises a resuscitation chamber body (11) and a resuscitation chamber cover (12) movably connected to the resuscitation chamber body (11), the resuscitation chamber cover (12) covers the resuscitation chamber body (11) to form a closed resuscitation space, the resuscitation chamber body (11) is provided with a dual-function rewarming tray (111), and the resuscitation chamber cover (12) is provided with a dual-function rewarming heat cover (121); the bottom support type mixing device (3) is arranged in the base (2) and connected to the dual-function rewarming tray (111) in the resuscitation chamber body (11), the resuscitation chamber cover (12) is also provided with an exhaust fan (123), and the base (2) is provided with a numerical control device (21), a communication device (22) and a touch screen (23); Wherein: a buffer device (122) and a pressure sensor (124) are provided between the dual-function rewarming thermal cover (121) and the resuscitation cabin cover (12); The dual-function rewarming tray (111) comprises a tray frame (1111) and a first heating body (1112); the first heating body (1112) is arranged in the tray frame (1111); the first heating body (1112) is an electric heating plate having both conduction heating and far-infrared radiation heating functions; the tray frame (1111) is provided with a first temperature control temperature sensor (1113); the first heating body (1112) is provided with a through hole; a first rewarming monitoring temperature sensor (1114) is provided in the through hole; The operating temperature range of the dual-function rewarming tray (111) is 10° C. to 70° C., and a guide line for placing a cell cryopreservation bag or a cell cryopreservation tube is drawn on the dual-function rewarming tray (111); The dual-function rewarming heat cover (121) comprises a heat cover frame (1211) and a second heating body (1212); the heat cover frame (1211) is made of metal; the second heating body (1212) is located in the heat cover frame (1211); the second heating body (1212) is an electric heating plate having both conduction heating and far-infrared radiation heating functions; the operating temperature range of the dual-function rewarming heat cover (121) is 10°C to 70°C; a guide line for placing a cell freezing bag or a cell freezing tube is drawn on the dual-function rewarming heat cover (121); the heat cover frame (1211) is provided with a second temperature control temperature sensor (1213); the second heating body (1212) is provided with a through hole; a second rewarming monitoring temperature sensor (1214) is provided in the through hole; The bottom support type mixing device (3) comprises a power source (31) and a power bracket (32), wherein the power source (31) is fixedly connected to the bottom of the base (2), the power source (31) comprises a servo motor (311) and a motor fixing bracket (312), an eccentric wheel (313) is provided at the motor shaft end of the servo motor (311), the number of the servo motors (311) is 4, the number of the power brackets (32) is 4, and the number of the eccentric wheels (313) is There are four eccentric wheels (313), the size specifications of the four eccentric wheels (313) are the same, and the initial eccentric distances of the four eccentric wheels (313) in the vertical direction are adjusted respectively during installation; the power bracket (32) movably connects the eccentric wheels (313) with the dual-function rewarming tray (111); the operation of the servo motor (311) is regulated by the numerical control device (21); and the bottom support type mixing device (3) drives the dual-function rewarming tray (111) to realize the bottom support type kneading oscillating mixing movement.

2. The apparatus for anhydrous dry cell resuscitation according to claim 1, characterized in that: The dual-function rewarming tray (111) is detachable and replaceable, the dual-function rewarming thermal cover (121) is movably connected to the resuscitation cabin cover (12) via the buffer device (122) and the pressure sensor (124), and the dual-function rewarming thermal cover (121) is detachable and replaceable.

3. The apparatus for anhydrous dry cell resuscitation according to claim 2, characterized in that: Air is sucked in from the vents on the bottom, side walls and rear wall of the base (2), rises along the gap inside the cavity of the resuscitation cabin (1), and is exhausted by the exhaust fan (123).

4. The apparatus for anhydrous dry cell resuscitation according to claim 2, characterized in that: The tray frame (1111) is made of metal, the first heating body (1112) is fixedly connected to the tray frame (1111), the number of the first temperature control temperature sensors (1113) is 1 to 6, and the number of the first retemperature monitoring temperature sensors (1114) is 1 to 6.

5. The apparatus for anhydrous dry cell resuscitation according to claim 2, characterized in that: The second heating body (1212) is fixedly connected to the heat cover frame (1211), the number of the second temperature control temperature sensors (1213) is 1 to 6, and the number of the second retemperature monitoring temperature sensors (1214) is 1 to 6.

6. The apparatus for anhydrous dry cell resuscitation according to claim 1, characterized in that: The communication device (22) is connected to the Internet or a local area network via wired or wireless networking to perform data exchange, data storage and data query.

7. A working method of an instrument device for anhydrous dry cell resuscitation based on any one of claims 1 to 6, wherein: The steps include: (1) Start the instrument, set the required temperature for recovery, select the recovery working mode, turn on the preheating function, and the dual-function recovery tray and the dual-function recovery cover begin to preheat. The temperature of the dual-function recovery tray and the dual-function recovery cover begins to rise and emit far-infrared rays. After the preheating is completed, place the cell cryopreservation bag or cell cryopreservation tube on the dual-function recovery tray, cover and fix the recovery cabin cover. Under the action of the buffer device between the dual-function recovery cover and the recovery cabin cover, the cell cryopreservation bag or cell cryopreservation tube is clamped by the dual-function recovery tray and the dual-function recovery cover. Cell recovery begins, and the dual-function recovery tray and the dual-function recovery cover move upward. The cell cryopreservation bag or cell cryopreservation tube transfers heat through conduction and radiation at the same time, and the bottom support type mixing device starts to work. Under the control of the numerical control device, the four eccentric wheels have different starting working angles and running speeds, driving the dual-function rewarming tray, cell cryopreservation bag or cell cryopreservation tube, and dual-function rewarming hot cover to perform bottom support type oscillation mixing movement. The force applied by the mixing movement can be detected by the pressure sensor between the dual-function rewarming hot cover and the recovery cabin cover, and transmitted to the numerical control device, which intelligently feedbacks and adjusts the operation of the power source to prevent excessive force from damaging the cell cryopreservation bag or instrument. (2) The temperature of the dual-function rewarming tray and the dual-function rewarming hot cover is monitored at any time by the temperature control temperature sensor, and the detection data is transmitted to the numerical control device. The numerical control device controls the heating process of the dual-function rewarming tray and the dual-function rewarming hot cover and intelligently maintains the set temperature; (3) The rewarming monitoring temperature sensor detects whether the current temperature of the cell cryopreservation bag or cell cryopreservation tube matches the set rewarming target temperature. The matching stability judgment time is no more than 5 seconds. If it is lower than the rewarming target temperature, the heating rewarming state is turned on and maintained. If it is higher than the rewarming target temperature, the heating is stopped immediately. (4) When in heating mode, the dual-function heating tray and the dual-function rewarming heat cover are in the heating state, the temperature of the tray and the heat cover rises, and far infrared rays are emitted. The heat is transferred to the cell cryopreservation bag or cell cryopreservation tube through two heat conduction methods, conduction and radiation, for rewarming; (5) When the rewarming monitoring temperature sensor detects that the temperature of the cell cryopreservation bag exceeds 0℃~8℃, it is judged that the ice in the cell cryopreservation bag or cell cryopreservation tube is completely melted, the heating mode is turned off, the dual-function rewarming tray and the dual-function rewarming heat cover stop working, and the exhaust fan is started at the same time. Air is sucked in from the vents on the bottom, side wall and rear wall of the base, rises along the gap inside the resuscitation chamber, and is finally discharged from the exhaust fan, taking away the heat of the dual-function rewarming tray and the dual-function rewarming heat cover. The bottom support mixing device continues to perform bottom support oscillation mixing motion; (6) When the cell resuscitation work in the cell cryopreservation bag or cell cryopreservation tube is completed, the indicator light flashes and the speaker sounds to remind the operator to take away the resuscitated cell cryopreservation bag or cell cryopreservation tube in time.

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