A micro-liquid mixing reaction device

Abstract

The present invention relates to the field of new material research and development, and is a micro-liquid mixing reaction device comprising a computer, a laser, a lens group, a camera, a base, a rotary motor, a rotary shaft, a rotary plate, a liquid mixer and a limit screw. The liquid mixer comprises a glass slide, a sample pool I, a sample pool II, a microchannel I, a microchannel II, a mixing chamber, a microchannel III and a liquid storage tank. The device has a specially designed microfluidic chamber and microchannel structure, adopts a mixing method based on centrifugal force, can quickly mix reactant liquids with volumes from nanoliter to microliter, and can be used for mixing and reacting small-volume fluid reaction samples. The microfluidic unit based on centrifugal force can be used for continuous mixing and reacting small-volume fluid samples of less than 30 microliters. The device has a good mixing effect on small-volume fluid samples, is low in cost, and is easy to operate.

Description

Technical Field

[0001] The present invention relates to the field of new material research and development, and in particular to a micro-liquid mixing reaction device capable of rapidly mixing reactant liquids in volumes ranging from nanoliters to microliters. Background Art

[0002] Thorough mixing of reactants is an important factor in efficient chemical reactions, especially in small-dose chemical analysis applications, where complete quantitative mixing is a determining factor in analytical accuracy. Numerous solutions exist in the prior art for mixing large quantities of reactants, but there is no low-cost method for fully mixing nanoliter to microliter quantities of reaction liquids. The micro-liquid mixing reaction device described herein can address this problem. Summary of the Invention

[0003] In order to solve the above problems, the device of the present invention has a microfluidic unit based on centrifugal force, which can be used for continuous mixing and reaction of small-volume fluid samples below 30 microliters.

[0004] The technical solution adopted in the present invention is:

[0005] The micro-liquid mixing reaction device comprises a computer, a laser, a lens group, a camera, a base, a rotary motor, a rotary shaft, a rotary plate, a liquid mixer and a limit screw, wherein xyz is a three-dimensional coordinate system, the liquid mixer comprises a glass slide, a sample pool I, a sample pool II, a microchannel I, a microchannel II, a mixing chamber, a microchannel III and a liquid storage tank, and there are two sets of liquid mixers; the laser and the camera are both connected to the computer, the lens group is located in front of the laser, the positions of the laser, the lens group and the camera are all adjustable, the rotary motor is installed inside the base, the rotation frequency of the rotary motor is adjustable, the rotary shaft is along the vertical y direction, and the lower part of the rotary shaft is The end is connected to the rotating motor and the upper end is exposed above the base; the rotating piece is a rectangular metal piece with a screw hole in the center. The rotating piece is connected to the upper end of the rotating shaft through the screw hole and is fixed by a limit screw. Two sets of liquid mixers are symmetrically installed on the rotating piece. The rotating motor can drive the two sets of liquid mixers to rotate in the xy horizontal plane; the camera is located 15 cm above the rotating piece and can transmit the collected light signal to the computer. The computer can adjust the time characteristics of the laser pulse emitted by the laser. The laser emitted by the laser can irradiate the liquid mixer after passing through the lens group; sample pool I, sample pool II, microchannel I, Microchannel II, mixing chamber, microchannel III and liquid reservoir are integrally formed from a single piece of polymer material and are tightly attached to the upper surface of the glass slide. One end of the mutually parallel microchannel I and microchannel II are connected to sample pool I and sample pool II respectively, and the other end is connected to the mixing chamber. The mixing chamber is connected to the liquid reservoir through microchannel III. Microchannel III is U-shaped in the xy horizontal plane. When the liquid passes through microchannel III, it forms a 180-degree bend. Microchannel III consists of three microchannels with an interval of 1.5 mm. The top of sample pool I and sample pool II has a liquid inlet with a diameter of 0.5 mm. The liquid inlet is for sample pool I. , sample pool II, microchannel I, microchannel II, mixing chamber, microchannel III and openings connecting the liquid reservoir to the atmosphere; the depths of sample pool I, sample pool II and the liquid reservoir are all 250 microns, the volumes of sample pool I and sample pool II are both 15 microliters, and the volume of the liquid reservoir is 40 microliters; the lengths of microchannel I and microchannel II are both 10 mm, and the cross-sections of microchannel I and microchannel II are both rectangles with a height of 250 microns and a width of 70 microns; the depth of the mixing chamber is 70 microns and the volume is 35 microliters; the cross-sections of the three microchannels of microchannel III are all rectangles with a height of 250 microns and a width of 50 microns.

[0006] The steps of conducting an experiment using the micro-liquid mixing reaction device are as follows:

[0007] Step 1: Use two pipettes to simultaneously fill 15 μl of the liquid I to be mixed and 15 μl of the liquid II to be mixed into the liquid inlets of sample pool I and sample pool II, respectively. Install the liquid mixer on the rotating plate so that the side of the liquid mixer where sample pool I and sample pool II are located is close to the center of the rotating plate;

[0008] Step 2: Turn on the rotary motor and adjust the rotation frequency of the rotary motor to increase linearly from 0 Hz to 10 Hz within 5 seconds. The mixed liquid I and the mixed liquid II in the sample pool I and sample pool II flow into the mixing chamber through the microchannel I and microchannel II respectively.

[0009] Step 3: Continue adjusting the rotational frequency of the rotary motor, linearly increasing it to 20 Hz over 8 seconds and maintaining it for 60 seconds. The liquid in the mixing chamber flows into the liquid reservoir through microchannel III, and some of the air originally in the liquid reservoir is compressed in the liquid reservoir.

[0010] Step 4: Continue to adjust the rotation frequency of the rotary motor and linearly reduce it to 0 Hz within 30 seconds. The compressed air in the liquid reservoir pushes the mixed liquid back into the mixing chamber.

[0011] Step 5: Repeat steps 2 to 4 three times to ensure that the liquid in the mixer is completely mixed and evenly distributed in the mixing chamber.

[0012] Step 6: Adjust the positions of the laser and the lens group so that the laser light emitted by the laser passes through the lens group and irradiates the mixing chamber of the liquid mixer;

[0013] Step 7: Adjust the position of the camera, collect images of the liquid in the liquid mixer and related optical information, and transmit the collected optical signals to the computer;

[0014] Step eight, analyzing and processing the optical signal in the computer to determine the characteristics of the product after the mixed liquid I and the mixed liquid II are mixed.

[0015] The beneficial effects of the present invention are:

[0016] The device of the present invention has a good mixing effect on a small volume of fluid sample, is low in cost, and is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The following is further described in conjunction with the graphics of the present invention:

[0018] Figure 1 It is a schematic diagram of the present invention;

[0019] Figure 2 1 is a top view of the rotating piece;

[0020] Figure 3It is an enlarged schematic diagram of a liquid mixer viewed from above.

[0021] In the figure, 1. Computer, 2. Laser, 3. Lens group, 4. Camera, 5. Base, 6. Rotating motor, 7. Rotating shaft, 8. Rotating plate, 9. Liquid mixer, 9-1. Glass slide, 9-2. Sample cell I, 9-3. Sample cell II, 9-4. Microchannel I, 9-5. Microchannel II, 9-6. Mixing chamber, 9-7. Microchannel III, 9-8. Liquid reservoir, 10. Limit screw. DETAILED DESCRIPTION

[0022] like Figure 1 It is a schematic diagram of the present invention, such as Figure 2 The invention is a top view schematic diagram of a rotating plate, comprising a computer (1), a laser (2), a lens group (3), a camera (4), a base (5), a rotating motor (6), a rotating shaft (7), a rotating plate (8), a liquid mixer (9) and a limit screw (10), wherein xyz is a three-dimensional coordinate system, the laser (2) and the camera (4) are both connected to the computer (1), the lens group (3) is located in front of the laser (2), the positions of the laser (2), the lens group (3) and the camera (4) are all adjustable, the rotating motor (6) is installed inside the base (5), the rotation frequency of the rotating motor (6) is adjustable, the rotating shaft (7) is along the vertical y direction, the lower end of the rotating shaft (7) is connected to the rotating motor (6), and the upper end is exposed. The rotating plate (8) is a rectangular metal plate with a screw hole at the center. The rotating plate (8) is connected to the upper end of the rotating shaft (7) through the screw hole and fixed by a limit screw (10). Two sets of liquid mixers (9) are symmetrically installed on the rotating plate (8). The rotating motor (6) can drive the two sets of liquid mixers (9) to rotate in the xy horizontal plane. The camera (4) is located 15 cm above the rotating plate (8) and can transmit the collected light signal to the computer (1). The computer (1) can adjust the time characteristics of the laser pulse emitted by the laser (2). The laser emitted by the laser (2) can irradiate the liquid mixer (9) after passing through the lens group (3).

[0023] like Figure 31 is an enlarged schematic diagram of a liquid mixer viewed from above. The liquid mixer (9) includes a glass slide (9-1), a sample pool I (9-2), a sample pool II (9-3), a microchannel I (9-4), a microchannel II (9-5), a mixing chamber (9-6), a microchannel III (9-7) and a liquid reservoir (9-8). The liquid mixer (9) has two sets, the sample pool I (9-2), the sample pool II (9-3), the microchannel I (9-4), the microchannel II (9-5), the mixing chamber (9-6), the microchannel III (9-7) and the liquid reservoir (9-8). A whole piece of polymer material is integrally processed and formed and is tightly attached to the upper surface of a glass slide (9-1). One end of mutually parallel microchannels I (9-4) and microchannel II (9-5) is connected to a sample pool I (9-2) and a sample pool II (9-3) respectively, and the other end is connected to a mixing chamber (9-6). The mixing chamber (9-6) is connected to a liquid storage tank (9-8) through a microchannel III (9-7). The microchannel III (9-7) is U-shaped in the xy horizontal plane. When the liquid passes through the microchannel III (9-7), a 180-degree bend is formed. The microchannel III (9- 7) consists of three microchannels with a spacing of 1.5 mm; each of the sample pool I (9-2) and the sample pool II (9-3) has a liquid inlet with a diameter of 0.5 mm, and the liquid inlet is an opening for the sample pool I (9-2), the sample pool II (9-3), the microchannel I (9-4), the microchannel II (9-5), the mixing chamber (9-6), the microchannel III (9-7) and the liquid reservoir (9-8) to communicate with the atmosphere; the depth of the sample pool I (9-2), the sample pool II (9-3) and the liquid reservoir (9-8) is 250 μm. The volumes of pool I (9-2) and sample pool II (9-3) are both 15 μl, and the volume of the liquid reservoir (9-8) is 40 μl; the lengths of microchannel I (9-4) and microchannel II (9-5) are both 10 mm, and the cross-sections of microchannel I (9-4) and microchannel II (9-5) are both rectangular with a height of 250 μm and a width of 70 μm; the depth of the mixing chamber (9-6) is 70 μm and the volume is 35 μl; the cross-sections of the three microchannels of microchannel III (9-7) are all rectangular with a height of 250 μm and a width of 50 μm.

[0024] The working principle of the device is as follows: 15 microliters of liquid I to be mixed and 15 microliters of liquid II to be mixed are respectively filled into the sample pool I (9-2) and the sample pool II (9-3) through their respective liquid inlets, the liquid mixer (9) is installed on the rotating plate (8), the side of the liquid mixer (9) where the sample pool I (9-2) and the sample pool II (9-3) are located is close to the center of the rotating plate (8), the rotating motor (6) is turned on, and the rotating motor (6) drives the liquid mixer (9) to rotate around the rotating axis (7) in the xy horizontal plane, and increases the speed of the rotating motor (6). When When the centrifugal force generated is greater than the capillary action of the liquid in the microchannel I (9-4) and the microchannel II (9-5), the liquid I to be mixed and the liquid II to be mixed in the sample pool I (9-2) and the sample pool II (9-3) flow to the mixing chamber (9-6) through the microchannel I (9-4) and the microchannel II (9-5) respectively. As the mixer (9) continues to rotate, the liquid in the mixing chamber (9-6) flows to the liquid reservoir (9-8) through the microchannel III (9-7), and a part of the air originally existing in the liquid reservoir (9-8) is compressed in the liquid reservoir (9-8). The pressure of the compressed air is Where V ca is the volume of the air compressed in the reservoir (9-8), V c is the volume of the liquid storage tank (9-8), and P0 is one atmospheric pressure; since the mixed liquid I and the mixed liquid II flow into the liquid storage tank (9-8) after mixing, the pressure of the compressed air increases. In order to keep the liquid flow rate in the mixing chamber (9-6) constant, it is necessary to increase the angular frequency of the mixer (9) rotation. Once there is no liquid in the sample pool I (9-2) and the sample pool II (9-3), the speed of the rotary motor (6) is adjusted, and the angular frequency of the mixer (9) rotation is gradually reduced, so that the centrifugal force decreases, and the compressed air in the liquid storage tank (9-8) pushes the mixed liquid back into the mixing chamber (9-6).

[0025] The micro-liquid mixing reaction device comprises a computer (1), a laser (2), a lens group (3), a camera (4), a base (5), a rotary motor (6), a rotary shaft (7), a rotary plate (8), a liquid mixer (9) and a limit screw (10), wherein xyz is a three-dimensional coordinate system, the liquid mixer (9) comprises a glass slide (9-1), a sample pool I (9-2), a sample pool II (9-3), a microchannel I (9-4), a microchannel II (9-5), a mixing chamber (9-6), a microchannel III (9-7) and a liquid storage pool (9-8), and there are two sets of liquid mixers (9); the laser (2) and the camera (4) are both connected to the computer ( 1), the lens group (3) is located in front of the laser (2), the positions of the laser (2), the lens group (3) and the camera (4) are all adjustable, the rotary motor (6) is installed inside the base (5), the rotation frequency of the rotary motor (6) is adjustable, the rotary shaft (7) is along the vertical y direction, the lower end of the rotary shaft (7) is connected to the rotary motor (6), and the upper end is exposed above the base (5); the rotary sheet (8) is a rectangular metal sheet with a screw hole in the center, the rotary sheet (8) is connected to the upper end of the rotary shaft (7) through the screw hole, and is fixed by a limit screw (10), two sets of liquid mixers (9) are symmetrically installed on the rotary sheet (8), and the rotary motor (6) can bring The two sets of liquid mixers (9) are rotated in the xy horizontal plane; the camera (4) is located 15 cm above the rotating plate (8) and can transmit the collected light signal to the computer (1). The computer (1) can adjust the time characteristics of the laser pulse emitted by the laser (2). The laser emitted by the laser (2) can irradiate the liquid mixer (9) after passing through the lens group (3); the sample pool I (9-2), the sample pool II (9-3), the microchannel I (9-4), the microchannel II (9-5), the mixing chamber (9-6), the microchannel III (9-7) and the liquid storage pool (9-8) are integrally formed by a whole piece of polymer material and are connected to the glass slide. The upper surface of the sheet (9-1) is tightly fitted, and one end of the mutually parallel microchannel I (9-4) and microchannel II (9-5) is connected to the sample pool I (9-2) and sample pool II (9-3) respectively, and the other end is connected to the mixing chamber (9-6). The mixing chamber (9-6) is connected to the liquid storage tank (9-8) through the microchannel III (9-7). The microchannel III (9-7) is U-shaped in the xy horizontal plane. When the liquid passes through the microchannel III (9-7), a 180-degree bend is formed. The microchannel III (9-7) is composed of three microchannels with an interval of 1.5 mm. The upper surface of the sample pool I (9-2) and the sample pool II (9-3) has a diameter of 0.The liquid inlet is 5 mm, and the liquid inlet is the opening for connecting the sample pool I (9-2), sample pool II (9-3), microchannel I (9-4), microchannel II (9-5), mixing chamber (9-6), microchannel III (9-7) and liquid reservoir (9-8) to the atmosphere; the depth of the sample pool I (9-2), sample pool II (9-3) and liquid reservoir (9-8) is 250 μm, and the volume of the sample pool I (9-2) and sample pool II (9-3) is 15 μm. The volume of the liquid reservoir (9-8) is 40 microliters; the length of microchannel I (9-4) and microchannel II (9-5) are both 10 mm, and the cross-section of microchannel I (9-4) and microchannel II (9-5) are both rectangular with a height of 250 microns and a width of 70 microns; the depth of the mixing chamber (9-6) is 70 microns and the volume is 35 microliters; the cross-section of the three microchannels of microchannel III (9-7) are all rectangular with a height of 250 microns and a width of 50 microns.

[0026] The device of the present invention has a specially designed microfluidic cavity and microchannel structure, adopts a mixing method based on centrifugal force, and can be used for mixing and reacting small-volume fluid reaction samples.

Claims

1. A micro-liquid mixing reaction device, comprising a computer (1), a laser (2), a lens group (3), a camera (4), a base (5), a rotary motor (6), a rotary shaft (7), a rotary plate (8), a liquid mixer (9) and a limit screw (10), wherein xyz is a three-dimensional coordinate system, and the liquid mixer (9) comprises a slide (9-1), a sample pool I (9-2), a sample pool II (9-3), a microchannel I (9-4), a microchannel II (9-5), a mixing chamber (9-6), and a plurality of other components. 6), microchannel III (9-7) and liquid storage tank (9-8), there are two sets of liquid mixers (9); the laser (2) and the camera (4) are connected to the computer (1), the lens group (3) is located in front of the laser (2), the positions of the laser (2), the lens group (3) and the camera (4) are all adjustable, the rotary motor (6) is installed inside the base (5), the rotary shaft (7) is along the vertical y direction, the lower end of the rotary shaft (7) is connected to the rotary motor (6), and the upper end is exposed above the base (5), Its characteristics are: The rotating plate (8) is a rectangular metal plate with a screw hole at the center. The rotating plate (8) is connected to the upper end of the rotating shaft (7) through the screw hole and is fixed by a limit screw (10). Two sets of liquid mixers (9) are symmetrically installed on the rotating plate (8). The rotating motor (6) can drive the two sets of liquid mixers (9) to rotate in the xy horizontal plane. The camera (4) is located 15 cm above the rotating plate (8) and can transmit the collected light signal to the computer (1). The computer (1) can adjust the time characteristics of the laser pulse emitted by the laser (2). The laser light emitted by the laser (2) can be irradiated to the liquid mixer (9) after passing through the lens group (3). The sample pool I (9-2), the sample pool II (9-3), the microchannel I (9-4), the microchannel II (9-5), the mixing chamber (9-6), the microchannel III (9-7) and the liquid storage tank (9-8) are integrally formed from a piece of polymer material and are tightly attached to the upper surface of the glass slide (9-1). One end of the mutually parallel microchannel I (9-4) and microchannel II (9-5) are connected to the sample pool I (9-2) and the sample pool II (9-3) respectively, and the other end is connected to the mixing chamber (9-6). The mixing chamber (9-6) is connected to the microchannel III (9 -7) is connected to the liquid storage tank (9-8), the microchannel III (9-7) is U-shaped in the xy horizontal plane, and the microchannel III (9-7) is composed of three microchannels with an interval of 1.5 mm; the sample pool I (9-2) and the sample pool II (9-3) are each provided with a liquid inlet with a diameter of 0.5 mm on the top, and the liquid inlet is an opening for connecting the sample pool I (9-2), the sample pool II (9-3), the microchannel I (9-4), the microchannel II (9-5), the mixing chamber (9-6), the microchannel III (9-7) and the liquid storage tank (9-8) with the atmosphere; The depths of sample pool I (9-2), sample pool II (9-3) and liquid reservoir (9-8) are all 250 microns, the volumes of sample pool I (9-2) and sample pool II (9-3) are all 15 microliters, and the volume of liquid reservoir (9-8) is 40 microliters; the lengths of microchannel I (9-4) and microchannel II (9-5) are both 10 mm, and the cross-sections of microchannel I (9-4) and microchannel II (9-5) are all rectangular with a height of 250 microns and a width of 70 microns; the depth of mixing chamber (9-6) is 70 microns and the volume is 35 microliters; the cross-sections of the three microchannels of microchannel III (9-7) are all rectangular with a height of 250 microns and a width of 50 microns; The working principle of the device is as follows: 15 microliters of liquid I to be mixed and 15 microliters of liquid II to be mixed are respectively filled into the sample pool I (9-2) and the sample pool II (9-3) through their respective liquid inlets, the liquid mixer (9) is installed on the rotating plate (8), the side of the liquid mixer (9) where the sample pool I (9-2) and the sample pool II (9-3) are located is close to the center of the rotating plate (8), the rotating motor (6) is turned on, and the rotating motor (6) drives the liquid mixer (9) to rotate around the rotating axis (7) in the xy horizontal plane, and increases the speed of the rotating motor (6). When When the centrifugal force generated is greater than the capillary action of the liquid in the microchannel I (9-4) and the microchannel II (9-5), the liquid I to be mixed and the liquid II to be mixed in the sample pool I (9-2) and the sample pool II (9-3) flow to the mixing chamber (9-6) through the microchannel I (9-4) and the microchannel II (9-5) respectively. As the mixer (9) continues to rotate, the liquid in the mixing chamber (9-6) flows to the liquid reservoir (9-8) through the microchannel III (9-7), and a part of the air originally existing in the liquid reservoir (9-8) is compressed in the liquid reservoir (9-8). The pressure of the compressed air is Where V ca is the volume of the air compressed in the reservoir (9-8), V c is the volume of the liquid storage tank (9-8), and P0 is one atmospheric pressure; since the mixed liquid I and the mixed liquid II flow into the liquid storage tank (9-8) after mixing, the pressure of the compressed air increases. In order to keep the liquid flow rate in the mixing chamber (9-6) constant, it is necessary to increase the angular frequency of the mixer (9) rotation. Once there is no liquid in the sample pool I (9-2) and the sample pool II (9-3), the speed of the rotary motor (6) is adjusted, and the angular frequency of the mixer (9) rotation is gradually reduced, so that the centrifugal force decreases, and the compressed air in the liquid storage tank (9-8) pushes the mixed liquid back into the mixing chamber (9-6).

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