A PCR test tube

Abstract

The present application provides a PCR test tube with a small tube cavity volume, which can reduce the reaction system, and at the same time can contact heat transfer over a large area and change temperature quickly. The whole tube is flat, including a tube body with an opening at the upper end and a tube cover for sealing the opening, and a tube cavity communicating with the opening is provided in the lower part of the tube body. There are two channels in the tube body; the lower ends of the two channels are in communication with the tube cavity, but the height of the part communicating with the tube cavity is different; the channel at a higher position in communication with the tube cavity is the air inlet channel, and the channel at a lower position in communication with the tube cavity is the reflux channel; the outlet at the upper end of the air inlet channel and the inlet at the upper end of the reflux channel are both located on the surface of the tube body in contact with the tube cover.

Description

Technical Field

[0001] The present application relates to experimental equipment in the field of biotechnology, and in particular to a PCR instrument and a PCR test tube. Background Art

[0002] At present, in the field of molecular biology, PCR reaction is a commonly used experimental technique. PCR reaction, namely polymerase chain reaction (PCR), is a method of in vitro enzymatic synthesis of specific DNA fragments.

[0003] The PCR (thermal cycler) instrument manufactured based on polymerase is actually a temperature control device. The PCR test tube (amplification tube, thin-walled tube, reaction tube) is a reaction vessel commonly used in the PCR instrument. A typical PCR amplification reaction is completed by repeatedly raising and lowering the temperature of the PCR test tube placed in the PCR instrument. In this process, the temperature is transferred from the PCR instrument to the PCR test tube placed in it by heating the metal block, and then the heat is transferred through the tube wall of the PCR test tube to the PCR reaction liquid in the tube, thereby triggering the amplification reaction.

[0004] CN201611132547.2 discloses a PCR test tube, comprising at least one tube body with an open end, wherein a heat conducting portion is provided on a part of the tube wall of the tube body. It prevents excessive reduction of the temperature inside the tube due to heat loss of the test tube by reducing the area of ​​the heat conducting portion. However, the PCR test tube is still very large in size, and the sample usage is at least several hundred microliters, and has a certain thermal inertia, and it takes a long time to heat or cool down.

[0005] CN201710061407.9 discloses a portable microfluidic PCR instrument, which can perform polymerase chain reaction, and can realize fluorescence quantitative detection of gene samples by collecting fluorescence images of reaction cycles through a fluorescence signal collection unit. It includes a control unit, a PCR chip (equivalent to a PCR test tube), and a temperature control unit and a fluorescence signal collection unit connected to the control unit; the PCR chip includes a substrate, a reaction chamber is formed on one end face of the substrate, and a sealing cover is provided on the reaction chamber, and the reaction chamber is connected to the outside through a capillary inlet and outlet sample hole provided on the substrate; the temperature control unit includes a temperature detector, and a heating part and a cooling part for heating or cooling the substrate in the PCR chip; the collection end of the fluorescence signal collection unit is arranged corresponding to one side of the sealing cover of the PCR chip to form the collection of the fluorescence image in the reaction chamber.

[0006] Since the reaction chamber is connected to the outside through the capillary injection hole and the sample outlet hole on the substrate (to achieve sample loading in the reaction chamber), it is difficult to process. Even if it can be processed, the cost is high and it is not suitable for promotion. Summary of the invention

[0007] The purpose of the present application is to provide a PCR test tube with a small tube cavity volume, which can reduce the reaction system, and at the same time has a large area of ​​contact heat transfer and a fast temperature change speed.

[0008] The technical solution to achieve the above purpose is: a PCR test tube, which is flat as a whole, including a tube body with an opening at the upper end and a tube cover for sealing the opening, and a tube cavity communicating with the opening is provided in the lower part of the tube body.

[0009] The above-mentioned PCR test tube has two channels in the tube body; the lower ends of the two channels are connected to the tube cavity, but the height positions of the parts connected to the tube cavity are different; the channel at a higher position when connected to the tube cavity is the air inlet channel, and the channel at a lower position when connected to the tube cavity is the reflux channel; the outlet at the upper end of the air inlet channel and the inlet at the upper end of the reflux channel are both located on the surface of the tube body in contact with the tube cover.

[0010] The above-mentioned PCR test tube has a transition channel in the tube cover; when the tube cover seals the opening, the air inlet channel and the reflux channel are connected through the transition channel.

[0011] The above-mentioned PCR test tube has a flange on the outer edge of the flat test tube, and the thickness of the flange is smaller than the thickness of the flat test tube.

[0012] In the above-mentioned PCR test tube, the flanges are located on the left and right sides of the flat test tube.

[0013] In the above-mentioned PCR test tube, the lower part of one side of the tube cover is connected to the tube body in an inverted manner.

[0014] The above-mentioned PCR test tube is made of light-transmitting material.

[0015] The present application also provides a PCR instrument, which can quickly complete the heating and cooling of PCR test tubes, thereby greatly improving the PCR reaction speed.

[0016] The PCR instrument described in this patent includes two hot plates opposite to each other on the left and right, which are equipped with heating devices and heated by the heating devices. A bracket is arranged between the left hot plate and the right hot plate; at least one of the above-mentioned PCR test tubes is arranged on the bracket; the bracket is connected to the left and right driving devices that drive it to move left and right; the bracket can move left or right under the drive of the left and right driving devices until the left side surface of the flat PCR test tube contacts the left hot plate surface, or the right side surface of the PCR test tube contacts the right hot plate surface.

[0017] As a further improvement to the above PCR instrument, when the left side of the PCR test tube contacts the left hot plate, a right air channel is formed between the right side of the PCR test tube and the right hot plate, so that the right side of the PCR test tube and the right hot plate are thermally insulated; when the right side of the PCR test tube contacts the right hot plate, a left air channel is formed between the left side of the PCR test tube and the left hot plate, so that the left side of the PCR test tube and the left hot plate are thermally insulated. Preferably, it includes a blowing device that allows air to flow through the left air channel or the right air channel.

[0018] As a further improvement to the above-mentioned PCR instrument, the PCR tubes on the bracket have m rows and n columns, where m and n are natural numbers greater than 1; the left sides of the PCR tubes are coplanar, and the right sides of the PCR tubes are coplanar.

[0019] As a further improvement to the above-mentioned PCR instrument, both hot plates are provided with light holes opposite to the PCR test tube cavities, and a fluorescence excitation module and a fluorescence receiving module are respectively arranged on the left and right sides of the two opposite hot plates; the excitation light emitted by the fluorescence excitation module passes through the light hole on one hot plate to irradiate the PCR test tube, and the fluorescence emitted by some substances in the reaction liquid in the irradiated PCR test tube cavity after being excited passes through the light hole on the other hot plate and is received by the fluorescence receiving module.

[0020] As a further improvement to the above-mentioned PCR instrument, the hot plate is made of a light-transmitting material; a fluorescence excitation module and a fluorescence receiving module are respectively arranged on the left and right sides of two opposite hot plates; the excitation light emitted by the fluorescence excitation module irradiates the PCR test tube through a hot plate, and the fluorescence emitted by some substances in the reaction liquid in the tube cavity of the irradiated PCR test tube after being excited passes through another hot plate and is received by the fluorescence receiving module.

[0021] As a further improvement to the above-mentioned PCR instrument, the fluorescence excitation module includes a light source, a first light collecting mirror, an excitation light filter, and a second light collecting mirror; the fluorescence receiving module includes a third light collecting mirror, a fluorescence filter, an imaging lens, and a camera; the light emitted by the light source is emitted as parallel light after passing through the first light collecting mirror, and then irradiated to each PCR test tube through the excitation light filter, the second light collecting mirror, and the hot plate; the fluorescence emitted by the substance in the reaction solution in each PCR test tube after excitation enters the imaging lens through the third light collecting mirror and the fluorescence filter and converges on the target surface of the camera. Preferably, the excitation light filter includes a plurality of excitation light filters capable of transmitting different wavelengths, and each excitation light filter is evenly distributed on the rotating first disk; the fluorescence filter includes fluorescence filters capable of transmitting different wavelengths corresponding to different excitation light filters; each fluorescence filter is evenly distributed on the rotating second disk; the first disk and the second disk rotate synchronously.

[0022] As a further improvement to the above-mentioned PCR instrument, the fluorescence excitation module includes a light source, a collimating lens, and an excitation light filter; the fluorescence receiving module includes a fluorescence filter and a collecting lens; the light emitted by the light source is emitted as parallel light after passing through the collimating lens, and then irradiates the reaction liquid in the PCR test tube through the excitation light filter; the fluorescence emitted by some substances in the reaction liquid after being excited passes through the fluorescence filter and is then converged by the collecting lens for people to observe or converged onto a photoelectric sensor.

[0023] Beneficial effects of this patent:

[0024] The thin-sheet test tube has a small tube cavity volume, which can reduce the size of the reaction system and at the same time provide a large area for contact heat transfer.

[0025] The left and right channels of the test tube are connected with the transition channel to form an internal circulation channel. When the sample is heated and reacted, the evaporated sample enters the air inlet channel from the lower port of the higher right channel, and flows back into the tube cavity through the transition channel and the reflux channel to form a circulation.

[0026] The test tube is inserted into the bracket. After insertion, the bracket can stably clamp the test tube and ensure that the two side surfaces of the bracket are lower than the left and right sides of the test tube. In this way, the bracket can stably press the left and right sides of the test tube against the surfaces of the two hot plates, so that good heat transfer can be achieved between the hot plate and the test tube.

[0027] The insertion structure on the bracket in the PCR instrument allows the test tube to participate in the reaction as soon as it is inserted. It has the characteristics of plug-and-react and is especially suitable for emergency use.

[0028] The air channel in the PCR instrument can ensure that the temperature of the hot plate that is not in contact with the test tube will not have a significant impact on the temperature of the test tube.

[0029] For a constant temperature hot plate two-stage rapid PCR instrument, the two hot plates are in a constant temperature state, and there is no temperature increase or decrease process, which can greatly increase the reaction speed.

[0030] For the three-stage rapid PCR instrument, the reaction and the temperature increase / lowering are carried out simultaneously and overlapped, thereby saving reaction time and increasing the test speed.

[0031] When the test tubes in the PCR instrument are being heated and reacted, the fluorescence excitation module can emit excitation light in real time, and the fluorescence receiving module can receive fluorescence in real time. It is a real-time fluorescence PCR detector.

[0032] The PCR instrument uses a transmissive fluorescence module system, which can obtain greater excitation energy, as well as higher excitation sensitivity and signal-to-noise ratio. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a front view of the PCR instrument of Example 1;

[0034] Figure 2 for Figure 1 A top view of

[0035] Figure 3 This is a schematic diagram of a PCR test tube;

[0036] Figure 4 for Figure 3 CC section view;

[0037] Figure 5 for Figure 4 BB cross-sectional view;

[0038] Figure 6 This is a schematic diagram of a PCR test tube;

[0039] Figure 7 It is a top view of the PCR tube connected to the support;

[0040] Figure 8 This is a front view of the PCR instrument of Example 2 ( Fig. 9 AA section view);

[0041] Fig. 9 for Figure 8 A top view of

[0042] Fig.10 Schematic diagram of fluorescence excitation module;

[0043] Fig.11 This is a schematic diagram of the fluorescence receiving module;

[0044] Fig.12 This is a schematic diagram of the PCR instrument of Example 3;

[0045] Fig.13 This is the schematic diagram of the transmissive fluorescence module system;

[0046] Fig.14 This is the schematic diagram of the reflective fluorescent module system. DETAILED DESCRIPTION

[0047] The technical solution will be described clearly and completely below in conjunction with the drawings in the embodiments. Obviously, the described embodiments are only a part of the embodiments, not all of the embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this patent.

[0048] Example 1: PCR instrument

[0049] like Figure 1 , 2The PCR instrument shown includes two left and right opposite left and right hot plates 1 and right hot plates 2, which have electric heating devices (belonging to the prior art, not shown) inside and are heated by the heating devices. A bracket 3 is arranged between the left and right hot plates 1 and 2; the bracket 3 is provided with 5 vertical slots 32, and the opposite side walls of the slots are provided with grooves 31. Four PCR test tubes 4 are inserted into each slot. Four rows and five columns of flat PCR test tubes 4 are inserted into the entire bracket 3. The bracket 3 is connected to the left and right driving devices (belonging to the prior art) 5 that drive it to move left and right; the bracket can move left or right under the drive of the left and right driving devices until the coplanar left side surfaces of the PCR test tubes are in contact with the left hot plate 1, or the coplanar right side surfaces of the PCR test tubes are in contact with the right hot plate 2.

[0050] When the left side of each PCR test tube contacts the left hot plate, a right air channel 12 is formed between the right side of each PCR test tube and the right hot plate, so that thermal insulation is achieved between the right side of each PCR test tube and the right hot plate; when the right side of each PCR test tube contacts the right hot plate, a left air channel 11 is formed between the left side of each PCR test tube and the left hot plate, so that thermal insulation is achieved between the left side of the PCR test tube and the left hot plate.

[0051] When the left side of the test tube contacts the left hot plate, the right air channel 12 can ensure that the temperature of the right hot plate will not have a significant impact on the temperature of the test tube in contact with the left hot plate; when the right side of the test tube contacts the right hot plate, the left air channel 11 can ensure that the temperature of the left hot plate will not have a significant impact on the temperature of the test tube in contact with the right hot plate. In order to further reduce the impact of the heat radiation of the hot plate that is not in contact with the test tube on the test tube, the air in the air channel can be blown by a blowing device such as a fan, so that a small amount of air convection is generated in the air channel.

[0052] See also Figure 3-7 The PCR test tube 4 shown is made of a light-transmitting material, and is flat as a whole, including a tube body 41 with an opening 411 at the upper end and a tube cover 42 for blocking the opening 411, and the lower left side of the tube cover is connected to the tube body in an inverted manner. There is an opening plug 421 protruding downward in the middle of the tube cover, and when the opening plug 421 extends into the opening 411 on the tube body, it can block the opening. There are flanges 43 on the outer edges of the left and right sides of the flat test tube, and the thickness of the flange is less than the thickness of the flat test tube. When the PCR test tube 4 is installed on the bracket 3, the flanges 43 on both sides are inserted into the grooves 31 on the bracket 3, which is convenient for installing or removing the PCR test tube from the bracket. In this structure, the test tube is inserted into the bracket, and after insertion, the bracket can stably clamp the test tube and ensure that the two side surfaces of the bracket are lower than the left and right sides of the test tube, so that the bracket can stably press the left and right sides of the test tube on the surface of the two hot plates, so that good heat transfer can be achieved between the hot plates and the test tube.

[0053] A lumen 44 communicating with the opening is formed in the lower inner portion of the tube body.

[0054] There are two channels, a left channel 45 and a right channel 46, on the left and right sides of the tube body 41. The lower ends of the two channels are in communication with the tube cavity, but the height of the parts communicating with the tube cavity is different. The right channel 46, which is at a higher position 461 communicating with the tube cavity (the lower end of the right channel), is the air inlet channel, and the left channel, which is at a lower position 451 communicating with the tube cavity (the lower end of the left channel), is the return channel 45; the outlet 48 at the upper end of the air inlet channel and the inlet 47 at the upper end of the return channel are both located on the surface of the tube body in contact with the tube cover.

[0055] A transition channel 49 is opened in the pipe cover; when the opening is blocked by the opening plug 421, the air inlet channel and the return channel are connected through the transition channel.

[0056] The lumen 44 of each PCR tube contains a sample (reaction solution) 100 .

[0057] When using a PCR test tube, open the tube cover 42, add the sample to the test tube with a sample gun, and add the sample (reaction liquid) to the tube cavity 44 through the opening 411. At this time, the left channel 45 and the right channel 46 are connected to the atmosphere, and the air compression in the tube cavity will not hinder the addition of the sample to the tube cavity. After adding the sample, the sample liquid level 101 should be higher than the point where the left channel and the tube cavity are connected (the lower port of the left channel) 451, but lower than the point where the right channel 46 and the tube cavity are connected (the lower port of the right channel) 461. Then close the tube cover, and the opening plug 421 blocks the opening. At the same time, the left channel 45 and the right channel 46 are connected to the transition channel 49 to form an internal circulation channel. When the sample is heated for reaction, the evaporated sample enters the air inlet channel from the higher lower port of the right channel, and flows back into the tube cavity through the transition channel and the reflux channel to form a circulation.

[0058] Example 2: PCR instrument

[0059] See also Figure 8 , 9 The PCR instrument of the second embodiment shown is mainly based on the PCR instrument of the first embodiment, and a fluorescence excitation module 6 and a fluorescence receiving module 7 are added.

[0060] The left hot plate 1 and the right hot plate 2 are respectively provided with light holes 13 opposite to the lower part of the tube cavity 44 of each PCR test tube 4, and a fluorescence excitation module 6 for emitting excitation light is arranged at each light hole of the right hot plate 2, and a fluorescence receiving module 7 is arranged at each light hole of the left hot plate 1.

[0061] See also Fig.10 , 11The fluorescence excitation module 6 belongs to the prior art, and mainly includes an LED light source 61, a collimating lens 62, and an excitation light filter 63; the fluorescence receiving module 7 belongs to the prior art, and mainly includes a fluorescence filter 71, a collecting lens 72, and a photoelectric sensor 73; the light emitted by the LED light source is emitted as parallel light after passing through the collimating lens, and then passes through the light-through hole on the right hot plate after passing through the excitation light filter to irradiate the PCR tube, and the fluorescence emitted by some substances in the reaction solution 100 in the PCR tube after being excited passes through the light-through hole on the left hot plate, and then passes through the fluorescence filter and the collecting lens to converge onto the photoelectric sensor.

[0062] Example 3: PCR instrument

[0063] See also Fig.12 The PCR instrument of the embodiment 3 shown is mainly based on the PCR instrument of the embodiment 1, and a fluorescence excitation module 8 and a fluorescence receiving module 9 are added, and the left hot plate 1 and the right hot plate 2 are required to be made of light-transmitting materials.

[0064] A fluorescence excitation module 8 for emitting excitation light is arranged on the left side of the left hot plate, and a fluorescence receiving module 9 is arranged on the right side of the right hot plate.

[0065] The fluorescence excitation module 8 includes a light source 81, a first light collecting mirror 82, a reflector 83, a plurality of excitation light filters 85 capable of transmitting different wavelengths and evenly distributed on the circumference of a rotating first disk 84, and a second light collecting mirror 86; the fluorescence receiving module 9 includes a third light collecting mirror 91, fluorescence filters 93 capable of transmitting different wavelengths and evenly distributed on the circumference of a rotating second disk 92 corresponding to different excitation light filters, an imaging lens 94, and a camera 95; the first disk and the second disk are of the same size and rotate synchronously on the same axis.

[0066] The light emitted by the light source is emitted as parallel light after passing through the first light collecting mirror, and then reflected by the reflector into the excitation light filter, and then passes through the second light collecting mirror and the left hot plate to irradiate each PCR test tube 4; the fluorescence emitted by the substance in the reaction solution in each PCR test tube after being excited passes through the right hot plate, the third light collecting mirror, the fluorescence filter, enters the imaging lens, and converges onto the target surface of the camera.

[0067] Example 4: Constant temperature hot plate type two-stage rapid PCR instrument

[0068] The use process of the PCR instrument shown in Examples 1, 2 or 3 is that the left hot plate and the right hot plate respectively maintain the two different reaction temperatures T1 and T2 required by the reaction solution in the PCR test tube and keep them constant (constant temperature); insert the test tube into the bracket, and the left and right driving devices drive the bracket to move to the left so that the left side of each PCR test tube on the bracket is in close contact with the left hot plate. After staying for a predetermined reaction time, the left and right driving devices drive the bracket to move to the right so that the right side of each PCR test tube is in close contact with the right hot plate. After staying for a predetermined reaction time, the left and right driving devices drive the bracket to move to the left so that the left side of each PCR test tube is in close contact with the left hot plate, and so on. The cycle continues until the entire PCR reaction is completed. At this point, the PCR instrument in Examples 1-3 can be said to be a constant temperature hot plate type second-stage rapid PCR instrument.

[0069] Example 5: Three-stage rapid PCR instrument

[0070] Another use process of the PCR instrument shown in Example 1, 2 or 3 includes the following steps:

[0071] 1) The left hot plate reaches the first reaction temperature required by the reaction solution in the PCR test tube;

[0072] 2) The support moves to the left hot plate that has reached the first reaction temperature, so that the left side of the PCR tube contacts the left hot plate and stays there for a certain reaction time. At the same time, the temperature of the right hot plate begins to change to the second reaction temperature required by the reaction solution;

[0073] 3) Then the support moves to the right hot plate that has reached the second reaction temperature, so that the right side of the PCR test tube contacts the right hot plate that has reached the second reaction temperature, and stays for a certain reaction time. At the same time, the left hot plate that has reached the first reaction temperature changes its temperature to the third reaction temperature required by the reaction solution;

[0074] 4) Then, the support moves to the left hot plate that has reached the third reaction temperature, so that the left side of the left PCR test tube contacts the left hot plate that has reached the third reaction temperature and stays there for a certain reaction time. Meanwhile, the right hot plate that has reached the second reaction temperature changes its temperature to the first reaction temperature required by the reaction solution.

[0075] 5) The support moves toward the right hot plate that has reached the first reaction temperature, so that the right side of the PCR tube contacts the right hot plate that has reached the first reaction temperature, and stays there for a certain reaction time. Meanwhile, the left hot plate that has reached the third reaction temperature changes its temperature to the second reaction temperature required by the reaction solution;

[0076] 6) Then the support moves to the left hot plate that has reached the second reaction temperature, so that the left side of the PCR test tube contacts the left hot plate that has reached the second reaction temperature, and stays for a certain reaction time. At the same time, the right hot plate that has reached the first reaction temperature changes its temperature to the third reaction temperature required by the reaction solution;

[0077] 7) The support moves toward the right hot plate that has reached the third reaction temperature, so that the right side of the left PCR test tube contacts the right hot plate that has reached the third reaction temperature, and stays there for a certain reaction time. Meanwhile, the left hot plate that has reached the second reaction temperature changes its temperature to the first reaction temperature required by the reaction solution;

[0078] Repeat steps 2)-7) until the entire PCR reaction is completed.

[0079] At this point, the PCR instrument in Example 1-3 can be said to be a three-stage rapid PCR instrument.

[0080] For Examples 1-5, the insertable structure of the bracket allows the test tube to participate in the reaction as soon as it is inserted, and the PCR instrument can be in operation all the time. Different test tubes can be inserted into the bracket at different times, and participate in the reaction as soon as they are inserted. The operator records or the relevant control software automatically records the insertion time of each test tube, and displays the reaction status of each test tube on the relevant display screen, and the setting can prompt the status of the test tube that has completed the reaction. It can be said that it has the characteristics of plug-and-play reaction, and is particularly suitable for emergency use.

[0081] For Examples 1-5, the thin sheet-like test tube has a small tube cavity volume, which can reduce the size of the reaction system and at the same time allow for contact heat transfer over a large area.

[0082] For the constant temperature hot plate type two-stage rapid PCR instrument of Example 4, the two hot plates are in a constant temperature state without the process of increasing or decreasing the temperature, which can greatly increase the reaction speed.

[0083] For the three-stage rapid PCR instrument of Example 5, one hot plate is in contact with the test tube, and while the sample in the test tube is reacting, the other hot plate completes heating or cooling. In other words, the reaction and heating / cooling can be performed synchronously and overlapped at the same time, thereby saving reaction time and increasing the test speed.

[0084] For Examples 2-5, while each test tube is being heated and reacted, the fluorescence excitation module can emit excitation light in real time, and the fluorescence receiving module can receive fluorescence in real time, thereby achieving real-time detection of the status of each test tube. This can be said to be a real-time fluorescence PCR detector.

[0085] For Examples 2-5, the thin sheet-shaped test tube has a larger side area, which can increase the light aperture of the reaction sample and improve the sensitivity of real-time fluorescence PCR detection.

[0086] For Examples 2-5, this patent uses Fig.13The transmission type fluorescence module system shown, that is, the excitation light emitted by the fluorescence excitation module 6 and the fluorescence excitation module 8 is directly transmitted through the test tube 4, and the fluorescence emitted by some substances in the test tube after being excited is received by the fluorescence receiving module 7 and the fluorescence receiving module 9 through the test tube. Compared with the reflection type fluorescence module system, the transmission type fluorescence module system can obtain greater excitation energy, as well as higher excitation sensitivity and signal-to-noise ratio. For the schematic diagram of the reflection type fluorescence module system, see Fig.14 The excitation light emitted by the fluorescence excitation module 6 and the fluorescence excitation module 8 enters the test tube 4 after being reflected by the spectroscope 14 . The fluorescence emitted by some substances in the test tube after being excited passes through the test tube and then passes through the spectroscope 14 and is received by the fluorescence receiving module 7 and the fluorescence receiving module 9 .

Claims

1. A PCR test tube, characterized in that: The whole is flat, and comprises a tube body with an opening at the upper end and a tube cover for sealing the opening. The lower part of one side of the tube cover is connected to the tube body in an inverted manner, and the lower part of the tube body has a tube cavity communicating with the opening. There are two channels in the tube body; the lower ends of the two channels are connected to the tube cavity, but the heights of the channels connected to the tube cavity are different; the channel at a higher position when connected to the tube cavity is the air inlet channel, and the channel at a lower position when connected to the tube cavity is the reflux channel; the outlet at the upper end of the air inlet channel and the inlet at the upper end of the reflux channel are both located on the surface of the tube body in contact with the tube cover; the PCR test tube is made of light-transmitting material.

2. The PCR test tube according to claim 1, characterized in that A transition channel is opened in the pipe cover; when the pipe cover blocks the opening, the air inlet channel and the return channel are connected through the transition channel.

3. The PCR test tube according to claim 1, characterized in that: A flange is provided on the outer edge of the flat test tube, and the thickness of the flange is smaller than the thickness of the flat test tube.

4. The PCR test tube according to claim 3, characterized in that: The flanges are located on the left and right sides of the flat test tube.

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