Leukocyte four-classification analysis device and analysis method

Abstract

The present invention provides a four-classification analysis device for leukocytes, the device comprising a leukocyte cup, a positive and negative pressure pump, a diluent adding channel, and a hemolytic agent adding channel, wherein the diluent is added into the leukocyte cup through the diluent adding channel, and the hemolytic agent is added into the diluted leukocyte cup once through the hemolytic agent adding channel, and the positive and negative pressure pumps are connected to the bottom of the leukocyte cup through an air channel, and are used to blow gas into the leukocyte cup to convectively mix the diluted blood sample with the hemolytic agent. The present invention only needs to add the hemolytic agent once, so it can simplify the liquid path structure and reduce the consumption of the hemolytic agent.

Description

Technical Field

[0001] The present invention relates to the technical field of blood cell analysis, and in particular to a white blood cell four-classification analysis device and an analysis method. Background Art

[0002] It should be noted that the contents recorded in this section do not represent all prior art.

[0003] The blood cell analyzer is an in vitro diagnostic device that can be used for blood cell counting, white blood cell classification, and hemoglobin concentration measurement in clinical tests. The four-category blood cell analyzer currently on the market can classify white blood cells into four types of cells: lymphocytes, monocytes, granulocytes, and eosinophils. Currently, it is necessary to add hemolytic agents to the blood sample multiple times in order to achieve the classification effect, which will inevitably lead to complex fluid circuits, large equipment size, high hemolytic agent consumption, and increased costs. Summary of the invention

[0004] In view of this, in order to solve one of the technical problems in the related art to a certain extent, it is necessary to provide a white blood cell four-classification analysis device and analysis method, which only requires adding a hemolytic agent once, thereby simplifying the liquid path structure and reducing the consumption of the hemolytic agent.

[0005] The present invention provides a four-category analysis device for leukocytes, which comprises a leukocyte cup, a positive and negative pressure pump, a diluent adding channel, and a hemolytic agent adding channel. The diluent is added into the leukocyte cup through the diluent adding channel, and the hemolytic agent is added into the diluted leukocyte cup at one time through the hemolytic agent adding channel. The positive and negative pressure pumps are connected to the bottom of the leukocyte cup through an air channel, and are used to blow gas into the leukocyte cup to convectively mix the diluted blood sample with the hemolytic agent.

[0006] Furthermore, it also includes a diluter and a first three-way valve, the first piston of the diluter is connected to the main channel of the first three-way valve through a pipeline, the normally open channel of the first three-way valve is connected to the first suction pipe, and the normally closed channel of the first three-way valve is connected to the hemolytic agent addition channel.

[0007] Furthermore, it also includes a second three-way valve, the second piston of the diluter is connected to the main channel of the second three-way valve through a pipeline, the normally open channel of the second three-way valve is connected to the second suction pipe, and the normally closed channel of the second three-way valve is connected to the diluent addition channel.

[0008] Furthermore, the third piston of the diluter is connected to a sampling needle, and a blood sample is added into the white blood cell cup through the sampling needle.

[0009] Furthermore, it also includes a red blood cell cup, a blood sample is added into the white blood cell cup through a sampling needle, and part of the blood sample after the first dilution in the white blood cell cup is extracted into the red blood cell cup through the sampling needle, and a diluent is added into the red blood cell cup through the diluent adding channel for secondary dilution.

[0010] Furthermore, the positive and negative pressure pumps are also connected to the bottom of the red blood cell cup, and are used to blow gas into the red blood cell cup to remix the diluted blood sample with the added diluent.

[0011] Furthermore, a gas buffer chamber is provided between the positive and negative pressure pumps and the red blood cell cup, and the gas generated by the positive and negative pressure pumps first passes through the gas buffer chamber and then enters the red blood cell cup.

[0012] Furthermore, a gas buffer chamber is provided on the gas channel, and the gas generated by the positive and negative pressure pumps first passes through the gas buffer chamber and then enters the leukocyte cup.

[0013] The present invention also provides a method for four-classification analysis of leukocytes, the method comprising the following steps:

[0014] Adding a diluent into the leukocyte cup in advance to dilute the blood sample in the leukocyte cup;

[0015] After the dilution is completed, a hemolytic agent is added into the leukocyte cup at one time;

[0016] After a certain period of mixing, the lymphocytes, monocytes, and granulocytes in the white blood cells were counted and the hemoglobin was measured;

[0017] Then, the positive and negative pressure pumps are used to continuously introduce gas into the leukocyte cup to cause convection of the sample solution to promote the secondary effect of the hemolytic agent on the leukocytes;

[0018] Eosinophil counts were performed in the leukocytes and a leukocyte channel count was performed.

[0019] Furthermore, the method further comprises:

[0020] Before adding the hemolytic agent into the leukocyte cup, a portion of the diluted blood sample is drawn;

[0021] After the aspiration is completed, a hemolytic agent is added to the white blood cell cup and part of the aspirated blood sample is added to the red blood cell cup;

[0022] Continuously introducing gas into the red blood cell cup through the positive and negative pressure pumps to cause convection of the sample solution to promote secondary dilution;

[0023] The red blood cells and platelets of the blood sample in the red blood cell cup are counted to complete the red blood cell channel counting.

[0024] It can be seen from the above scheme that the present invention only adds hemolytic agent once within the measurement cycle of a sample, and can blow gas into the white blood cell cup through positive and negative pressure pumps, thereby strengthening the convection of the sample solution, promoting the secondary action of the hemolytic agent and the white blood cells, and simplifying the liquid path structure design, thereby achieving the purpose of completing the four classifications of white blood cells by adding the hemolytic agent once. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a liquid circuit diagram of a specific implementation mode of the present invention.

[0026] Figure 2 A flowchart of an analysis method provided for a specific embodiment of the present invention.

[0027] The following specific implementation manner will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the protection scope of the present invention. It is understood that the drawings are only provided for reference and illustration, and are not used to limit the present invention. The connection relationship shown in the drawings is only for the convenience of clear description and does not limit the connection method.

[0029] It should be noted that when a component is considered to be "connected" to another component, it can be directly connected to another component, or there may be an intermediate component at the same time. Unless otherwise defined, all technical and scientific terms used in this document have the same meaning as those generally understood by technicians in the technical field of the present invention. It should also be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0030] It should also be noted that in the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0031] See also Figure 1 A specific embodiment of the present invention provides a four-classification analysis device 100 for white blood cells, the device comprising a white blood cell cup 10, a positive and negative pressure pump 30, a diluent adding channel 11, and a hemolytic agent adding channel 12. The diluent is added into the white blood cell cup 10 through the diluent adding channel 11, and the hemolytic agent is added into the diluted white blood cell cup 10 at one time through the hemolytic agent adding channel 12. The positive and negative pressure pump 30 is connected to the bottom of the white blood cell cup 10 through the gas channel 31, and is used to blow a proper amount of gas into the white blood cell cup 10 at a suitable speed to mix the diluted blood sample with the hemolytic agent by convection.

[0032] In order to make the convection between the blood sample and the hemolytic agent more uniform and smooth, a first gas buffer chamber 32 is provided on the gas channel 31. The gas generated by the positive and negative pressure pumps 30 first passes through the first gas buffer chamber 32 and then enters the white blood cell cup 10. At the same time, the first gas buffer chamber 32 can also be used as an isolation device for isolating liquid.

[0033] In this embodiment, the device 100 further includes a diluter 121 and a first three-way valve 122. The first piston 1211 of the diluter 121 is connected to the main channel 1221 of the first three-way valve 122 through a pipeline. The normally open channel 1222 of the first three-way valve 122 is connected to the first suction pipe 123, and the normally closed channel 1223 of the first three-way valve 122 is connected to the leukocyte cup 10. The first suction pipe 123 may be provided with a hemolytic agent detection sensor 124 for detecting whether there is a hemolytic agent. If there is a hemolytic agent, the normally open channel 1222 of the first three-way valve 122 is in an open state, and the normally closed channel 1223 of the first three-way valve 122 is in a closed state. The first piston 1211 of the diluter 121 moves downward to suck the hemolytic agent in the hemolytic agent bottle into the diluter 121. When it is necessary to add a hemolytic agent into the leukocyte cup 10 , the normally open channel 1222 of the first three-way valve 122 is closed, the normally closed channel 1223 of the first three-way valve 122 is opened, and the first piston 1211 of the diluter 121 moves upward to add the hemolytic agent in the diluter 121 into the leukocyte cup 10 .

[0034] The second piston 1212 of the diluter 121 is connected to the main channel of the second three-way valve through a pipeline, the normally open channel of the second three-way valve is connected to the second suction pipe, and the normally closed channel of the second three-way valve is connected to the diluent adding channel 11. The second suction pipe may be provided with a diluent detection sensor for detecting whether there is a diluent. If there is a diluent, the normally open channel of the second three-way valve is in an open state, the normally closed channel 1223 of the first three-way valve 122 is in a closed state, and the second piston 1212 of the diluter 121 moves downward to suck the diluent into the diluter 121.

[0035] The third piston 1213 of the diluter 121 can be connected to a sampling needle 40. The third piston 1213 moves to add a blood sample into the white blood cell cup 10 through the sampling needle 40, and can extract part of the blood sample after the first dilution in the white blood cell cup 10 into the red blood cell cup 20 through the sampling needle 40, add a diluent into the red blood cell cup 20 through the diluent adding channel 11 for secondary dilution, and add a hemolytic agent into the white blood cell cup 10.

[0036] In this embodiment, the liquid circuit uses a diluter 121 and stores the diluent and the hemolytic agent at different time periods, thereby improving the utilization rate of the diluter 121 and saving the space of the analysis device 100.

[0037] Similarly, the positive and negative pressure pump 30 is also connected to the bottom of the red blood cell cup 20, and is used to blow gas into the red blood cell cup 20 to remix the diluted blood sample with the added diluent. Specifically, a second gas buffer chamber 33 is provided between the positive and negative pressure pump 30 and the red blood cell cup 20, and the gas generated by the positive and negative pressure pump 30 first passes through the second gas buffer chamber 33 and then enters the red blood cell cup 20.

[0038] The normally closed channel 1223 of the first three-way valve 122 is connected to one inlet of the three-way pipe 34, the gas channel 31 is connected to the other inlet of the three-way pipe 34, and the outlet of the three-way pipe 34 is connected to the leukocyte cup 10, so that the normally closed channel 1223 of the first three-way valve 122 and the gas channel 31 are connected to the leukocyte cup 10 through the three-way pipe 34, so that the liquid can be better isolated. In this embodiment, the outlet of the first gas buffer chamber 32 is connected to the other inlet of the three-way pipe 34 to improve the liquid isolation effect.

[0039] The present invention only adds hemolytic agent once during the measurement cycle of a sample, and can blow gas into the leukocyte cup 10 through the positive and negative pressure pumps 30, thereby strengthening the convection of the sample solution, promoting the secondary action of the hemolytic agent and the leukocytes, simplifying the design of the liquid path structure, and achieving the purpose of completing the four classifications of leukocytes by adding the hemolytic agent once.

[0040] The specific embodiment of the present invention also provides a method for four-classification analysis of leukocytes, such as Figure 2 As shown, the method may include the following steps.

[0041] S110 : Add diluent into the leukocyte cup 10 in advance to dilute the blood sample in the leukocyte cup 10 .

[0042] S120: After the dilution is completed, a hemolytic agent is added into the leukocyte cup 10 at one time.

[0043] S130: After mixing for a certain period of time, counting lymphocytes, monocytes and granulocytes in the white blood cells and measuring hemoglobin. The applicant has found that after mixing for a few seconds (eg, 3-10 seconds), the lymphocytes, monocytes and granulocytes in the white blood cells can be counted and the hemoglobin can be measured.

[0044] S140: Continuously introduce gas into the leukocyte cup 10 through the positive and negative pressure pumps 30 to cause convection of the sample solution to promote the secondary effect of the hemolytic agent on the leukocytes.

[0045] S150: Count the eosinophils in the white blood cells and complete the white blood cell channel count.

[0046] A specific embodiment of the present invention provides a method for four-category analysis of white blood cells. Before adding a hemolytic agent to the white blood cell cup 10, a portion of the diluted blood sample is first drawn. After the drawing is completed, the hemolytic agent is added to the white blood cell cup 10 and the drawn portion of the blood sample is added to the red blood cell cup 20. The transfer of the blood sample and the addition of the hemolytic agent are performed simultaneously, which saves analysis time. Then, an appropriate amount of diluent is added to the red blood cell cup 20 again through the diluent addition channel 11, and gas is continuously introduced into the red blood cell cup 20 through the positive and negative pressure pumps 30 to allow the sample solution to convect to promote secondary dilution. The red blood cells and platelets of the blood sample in the red blood cell cup 20 are counted to complete the red blood cell channel counting.

[0047] In the specification and claims of the present application, the words "include / comprise" and the words "have / include" and their variations are used to specify the existence of stated features, values, steps or components, but do not exclude the existence or addition of one or more other features, values, steps, components or combinations thereof.

[0048] Some features of the present invention are described in different embodiments for clarity of description, however, these features may also be described in combination in a single embodiment. Conversely, some features of the present invention are described in only a single embodiment for brevity, however, these features may also be described in different embodiments alone or in any suitable combination.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A four-classification analysis device for white blood cells, characterized in that: The device comprises a leukocyte cup, a positive and negative pressure pump, a diluent adding channel, and a hemolytic agent adding channel. The diluent is added into the leukocyte cup through the diluent adding channel, and the hemolytic agent is added into the diluted leukocyte cup at one time through the hemolytic agent adding channel. The positive and negative pressure pumps are connected to the bottom of the leukocyte cup through an air channel, and are used to blow gas into the leukocyte cup to mix the diluted blood sample with the hemolytic agent by convection. It also includes a diluter and a first three-way valve, wherein the first piston of the diluter is connected to the main channel of the first three-way valve through a pipeline, the normally open channel of the first three-way valve is connected to the first suction pipe, and the normally closed channel of the first three-way valve is connected to the hemolytic agent addition channel; It also includes a red blood cell cup, a blood sample is added into the white blood cell cup through a sampling needle, and a portion of the blood sample after the first dilution in the white blood cell cup is extracted into the red blood cell cup through the sampling needle, and a diluent is added into the red blood cell cup through the diluent adding channel for secondary dilution; The gas channel is provided with a gas buffer chamber, and the gas generated by the positive and negative pressure pumps first passes through the gas buffer chamber and then enters into the leukocyte cup.

2. The leukocyte four-classification analysis device according to claim 1, characterized in that: It also includes a second three-way valve, the second piston of the diluter is connected to the main channel of the second three-way valve through a pipeline, the normally open channel of the second three-way valve is connected to the second suction pipe, and the normally closed channel of the second three-way valve is connected to the diluent addition channel.

3. The leukocyte four-classification analysis device according to claim 1, characterized in that: The third piston of the diluter is connected with a sampling needle, and a blood sample is added into the leukocyte cup through the sampling needle.

4. The leukocyte four-classification analysis device according to claim 1, characterized in that: The positive and negative pressure pumps are also connected to the bottom of the red blood cell cup, and are used to blow gas into the red blood cell cup to remix the diluted blood sample with the added diluent.

5. The leukocyte four-classification analysis device according to claim 4, characterized in that: A gas buffer chamber is provided between the positive and negative pressure pumps and the red blood cell cup, and the gas generated by the positive and negative pressure pumps first passes through the gas buffer chamber and then enters the red blood cell cup.

6. A method for four-classification analysis of leukocytes, characterized in that: The method is applied to a leukocyte four-classification analysis device according to any one of items 1 to 5; the method comprises the following steps: Adding a diluent into the leukocyte cup in advance to dilute the blood sample in the leukocyte cup; After the dilution is completed, a hemolytic agent is added into the leukocyte cup at one time; After mixing for 3-10 seconds, lymphocytes, monocytes, and granulocytes in the white blood cells were counted and hemoglobin was measured; Then, the positive and negative pressure pumps are used to continuously introduce gas into the leukocyte cup to cause convection of the sample solution to promote the secondary effect of the hemolytic agent on the leukocytes; Eosinophil counts were performed in the leukocytes and a leukocyte channel count was performed.

7. The method for four-classification analysis of leukocytes according to claim 6, characterized in that: The method further comprises: Before adding the hemolytic agent into the leukocyte cup, a portion of the diluted blood sample is drawn; After the aspiration is completed, a hemolytic agent is added to the white blood cell cup and part of the aspirated blood sample is added to the red blood cell cup; Continuously introducing gas into the red blood cell cup through the positive and negative pressure pumps to cause convection of the sample solution to promote secondary dilution; The red blood cells and platelets of the blood sample in the red blood cell cup are counted to complete the red blood cell channel counting.

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