Camel hump composite ointment and its preparation method and application

Abstract

The present invention discloses a camel hump composite ointment, its preparation method, and application. Camel hump oil, egg yolk oil, emu oil, lavender essential oil, and traditional Chinese medicine extracts of cloves, Zanthoxylum bungeanum, gallic acid fruit, and dried ginger are mixed and processed in proportion to form an ointment formulation. The ointment formulation optimizes the composition, improves the transdermal absorption performance, uniformity, appearance quality, odor and color, and physical stability of the camel hump composite ointment, and increases its efficacy and activity. This ointment addresses the problems of crude ointments, such as poor absorption, limited ingredients, crude processing, poor physical stability, strong odor from camel hump oil, and inconvenience in use and portability. The camel hump composite ointment provided by the present invention is suitable for use in combination with therapeutic drugs and has a significant relieving and alleviating effect in the adjunctive treatment of osteoarthritis and rheumatoid arthritis pain.

Description

Technical Field

[0001] The present invention relates to the field of treating chronic pain caused by rheumatoid arthritis and osteoarthritis, in particular to a camel hump composite ointment and a preparation method and application thereof. Background Art

[0002] Chronic pain caused by rheumatoid arthritis and osteoarthritis affects a person's normal activities, work, and study, requiring long-term medication. It is a chronic disease that is difficult to cure. Long-term use of non-steroidal anti-inflammatory drugs and massage of Voltaren cream are common treatment methods. However, with long-term medication, it is difficult to avoid the side effects of Western medicine, and there is also the disadvantage of high economic costs. Camel hump compound ointment is an effective folk remedy for treating bone and joint pain. The preparation process of this compound ointment is to use camel hump oil as the main raw material, crush three medicinal materials such as pepper, cloves, and gallic acid into coarse powder, mix them, put them into camel hump oil, put them in a glass bottle, and place them in a sunny place for 15 to 20 days. Then, this medicinal ointment is applied to the joints of the body and massaged, and exposed to sunlight for about 1 hour. This method is used to assist in the treatment of symptoms such as osteoarthritis, joint pain, and bone pain, with good therapeutic effects.

[0003] However, the above process has the following disadvantages: (1) The ointment is not easily absorbed: most of it remains on the surface of the skin for a long time and requires long-term sunlight exposure to expand the skin for gradual absorption; (2) The formula ingredients are relatively simple and the processing technology is relatively rough: Camel hump oil is mainly used as an oily carrier and therapeutic agent, mixed with clove coarse powder (the main component is clove oil), gallic acid coarse powder (mainly gallic acid) and pepper coarse powder (the main component is pepper oil). The medicinal ingredients are dissolved under long-term sunlight exposure. After cooling, most of the medicinal residue coarse powder is deposited at the bottom. If they are directly applied to the skin, they are even less likely to be absorbed, affecting the uniformity, appearance quality and absorption and utilization of the ointment by the skin; (3) The physical stability is poor: The ointment in a solid state is easily liquefied in summer and has poor stability, which brings certain difficulties to the standard packaging and storage of liquid or solid; (4) The odor is relatively bad: Since camel hump oil has a certain fishy smell, the ointment prepared by the above process has a certain odor, which will bring certain inconveniences to work, study and life after use. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a camel hump composite ointment and its preparation method and application, which can solve the above-mentioned technical problems that the ointment is not easy to absorb, has a single formula, rough process, poor physical stability, and a relatively bad odor.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A camel hump composite ointment comprising the following raw materials in parts by weight:

[0007] 450-680 parts of camel hump oil, 25-85 parts of egg yolk oil, 80-295 parts of emu oil, 50-250 parts of beeswax, 15-95 parts of Chinese herbal extracts and lavender essential oil;

[0008] The Chinese herbal medicine extracts include cloves, gallic acid, Zanthoxylum bungeanum and dried ginger. The mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is (9-15): (12-23): (8-32): (16-48);

[0009] The mass ratio of camel hump compound ointment to lavender essential oil is (290-428):1.

[0010] The present invention also provides a method for preparing the camel hump composite ointment, which is characterized by comprising the following steps:

[0011] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 85-150°C for 3-7 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0012] 2) Cloves, gallic acid seeds, Zanthoxylum bungeanum, and dried ginger were ground separately and passed through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing according to the proportion, ethanol was added for extraction, and the mixture was concentrated under reduced pressure to obtain a Chinese herbal medicine extract;

[0013] 3) 450-680 parts of the camel hump oil obtained in step 1) are heated to 65-135° C., and then 25-85 parts of egg butter, 80-295 parts of emu oil, 50-250 parts of beeswax, 15-95 parts of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil are added. The mixture is heated with stirring for 1.1-3.5 hours, cooled and aliquoted to obtain a camel hump composite ointment.

[0014] Preferably, in step 2), the relative density of the Chinese herbal medicine extract after concentration under reduced pressure is 0.91 to 1.25.

[0015] Preferably, in step 2), ethanol extraction is performed twice.

[0016] Preferably, in step 2), the mass concentration of ethanol used in the two extractions is 35-85%.

[0017] Preferably, in step 3), the amount of ethanol added in the two extractions is (10-28):1 based on the total mass of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger.

[0018] The present invention also provides use of the camel hump composite ointment in treating chronic pain caused by rheumatoid arthritis and osteoarthritis.

[0019] Compared with traditional composite ointments, the advantages of the camel hump composite ointment provided by the present invention and its preparation method and application are as follows:

[0020] 1) Ingredients were added and the formula was optimized: Camel hump oil was used as the main oily ingredient, and egg yolk oil and emu oil, which have certain anti-inflammatory and analgesic effects and are good at promoting transdermal absorption, were added. This significantly accelerated the speed and degree of skin absorption of the ointment under room temperature massage conditions;

[0021] 2) Improved preparation process: Cloves, gallic acid seeds, Sichuan peppercorns, and dried ginger are crushed and sieved into fine powders, extracted with ethanol, and then the extract is mixed with camel hump oil in appropriate proportions to form a uniform system, which significantly improves the appearance, color, and uniformity.

[0022] 3) Improved physical stability: By adding beeswax to the Camel Hump compound ointment, the physical stability is improved. In hot summer weather, the Camel Hump compound ointment will not liquefy and maintain its original shape. This allows the Camel Hump compound ointment to be qualified according to the standards for solid ointments and is easy to package, store and transport.

[0023] 4) Eliminate fishy smell: The addition of lavender essential oil can make the camel hump compound ointment have a light fragrance without fishy smell. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The present invention is a flow chart for the preparation of the camel hump composite ointment. DETAILED DESCRIPTION

[0025] The present invention provides a camel hump composite ointment comprising the following raw materials in parts by weight:

[0026] 450-680 parts of camel hump oil, 25-85 parts of egg yolk oil, 80-295 parts of emu oil, 50-250 parts of beeswax, 15-95 parts of Chinese herbal extracts and lavender essential oil;

[0027] The Chinese herbal medicine extracts include cloves, gallic acid, Zanthoxylum bungeanum and dried ginger. The mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is (9-15): (12-23): (8-32): (16-48);

[0028] The mass ratio of camel hump compound ointment to lavender essential oil is (290-428):1.

[0029] The present invention combines camel hump oil, egg yolk oil, emu oil, lavender essential oil, and traditional Chinese medicine extracts of cloves, Zanthoxylum bungeanum, gallic acid fruit, and dried ginger in a proportioned mixture to produce an ointment formulation. This optimized formulation improves the transdermal absorption, uniformity, appearance, odor, color, and physical stability of the camel hump composite ointment, thereby enhancing its efficacy and activity. This ointment addresses the problems of crude ointments such as poor absorption, limited ingredients, crude processing, poor physical stability, a strong odor from camel hump oil, and inconvenience in use and portability. The camel hump composite ointment provided by the present invention is suitable for use in combination with therapeutic drugs and effectively alleviates and relieves pain in the adjunctive treatment of osteoarthritis and rheumatoid arthritis.

[0030] Camel hump oil is used to treat osteoarthritis and pain caused by bone and joints. In an embodiment of the present invention, the weight of camel hump oil is 450 to 680 parts; in a preferred embodiment, the weight of camel hump oil is 500 to 600 parts.

[0031] The egg butter has the effects of relieving pain, moisturizing, and nourishing. In an embodiment of the present invention, the egg butter has an amount of 25 to 85 parts by weight; in a preferred embodiment, the egg butter has an amount of 50 to 70 parts by weight.

[0032] Emu oil promotes skin absorption of the Camelback compound ointment and has excellent anti-inflammatory and burn scar repairing effects. In embodiments of the present invention, the emu oil is present in an amount of 80 to 295 parts by weight; in a preferred embodiment, the emu oil is present in an amount of 150 to 250 parts by weight.

[0033] The weight proportion of beeswax is 50 to 250 parts; in a preferred embodiment, the weight proportion of beeswax is 100 to 200 parts.

[0034] The mass ratio of camel hump compound ointment to lavender essential oil is (290-428):1.

[0035] The Chinese herbal medicine extract includes cloves, gallic acid, Zanthoxylum bungeanum and dried ginger. The mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is (9-15): (12-23): (8-32): (16-48). All of the above medicinal materials have pharmacologically active ingredients.

[0036] Cloves have the benefits of warming the middle and relieving adverse reactions, promoting qi circulation and relieving pain, and warming the kidneys and strengthening yang. Cloves are both medicinal and edible, with their primary active ingredient being clove oil, which contains natural medicinal compounds such as eugenol. Cloves have certain antipyretic, analgesic, and anesthetic properties, with a pronounced analgesic effect. Eugenol's antipyretic effect is stronger than that of the Western medicine acetaminophen.

[0037] Gallic acid is a traditional Chinese medicine that has the properties of strengthening Qi, astringing essence, strengthening the lungs, and stopping bleeding. It can treat yin sweating, toothache, bleeding from trauma, and persistent sores. Gallic acid is the active ingredient in gallic acid, which is soluble in ethanol.

[0038] Zanthoxylum bungeanum has the effects of warming the middle and dispelling cold, removing dampness, relieving pain, and killing insects. It can treat cold pain in the heart and abdomen, wind-cold and dampness, hernia pain, toothache, and vaginal itching. The main active ingredient of Zanthoxylum bungeanum is fat-soluble (or ether-soluble) Zanthoxylum bungeanum oil, which is soluble in ethanol and uniformly miscible with oily matrices such as camel hump oil.

[0039] Dried ginger has the effects of warming the middle and dispersing cold, restoring yang and unblocking meridians, drying dampness and eliminating phlegm.

[0040] Combine Figure 1 The present invention also provides a method for preparing the camel hump composite ointment, comprising the following steps:

[0041] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 85-150°C for 3-7 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0042] 2) Cloves, gallic acid seeds, Zanthoxylum bungeanum, and dried ginger were ground separately and passed through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing according to the proportion, ethanol was added for extraction, and the mixture was concentrated under reduced pressure to obtain a Chinese herbal medicine extract;

[0043] 3) 450-680 parts of the camel hump oil obtained in step 1) are heated to 65-135° C., and then 25-85 parts of egg butter, 80-295 parts of emu oil, 50-250 parts of beeswax, 15-95 parts of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil are added. The mixture is heated with stirring for 1.1-3.5 hours, cooled and aliquoted to obtain a camel hump composite ointment.

[0044] In an embodiment of the present invention, the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 0.91-1.25.

[0045] To fully extract the active substances in cloves, gallic acid, Zanthoxylum bungeanum, and dried ginger, ethanol extraction was performed twice. The mass concentration of ethanol used in both extractions was 35-85%; the amount of ethanol added in both extractions was (10-28):1 to the total mass of cloves, gallic acid, Zanthoxylum bungeanum, and dried ginger.

[0046] The present invention heats and melts camel hump to extract oil, then adds emu oil, egg yolk oil, beeswax, and the aforementioned Chinese herbal extracts, along with lavender essential oil, and uniformly mixes before cooling to produce a camel hump composite ointment. The present invention has a simple preparation process, is environmentally friendly, exhibits significant efficacy, significantly improves drugability, is easy to use, has a fragrant aroma, and is portable. It is particularly suitable for the adjunctive treatment of chronic conditions such as osteoarthritis and osteoarthritis pain in patients of all ages, and is a safe, effective, and green composite ointment. Furthermore, the raw materials for this product are inexpensive and readily available, and its cost price is significantly lower than that of similar adjunctive treatment preparations, resulting in high practical value and market prospects.

[0047] The present invention also provides use of the camel hump composite ointment in treating chronic pain caused by rheumatoid arthritis and osteoarthritis.

[0048] The camel hump composite ointment provided by the invention can be used in combination with therapeutic drugs to assist in the treatment of osteoarthritis and rheumatic arthritis pain, and has a good relieving and alleviating effect.

[0049] To further illustrate the present invention, a camel hump composite ointment provided by the present invention, a preparation method thereof, and applications thereof are described in detail below in conjunction with examples, but they should not be construed as limiting the scope of protection of the present invention.

[0050] Example 1

[0051] Camel hump composite ointment comprises the following raw materials in parts by weight:

[0052] 450g camel hump oil, 25g egg butter, 80g emu oil, 50g beeswax, 15g Chinese herbal extract, g lavender essential oil;

[0053] The Chinese herbal medicine extract includes cloves, gallic acid, pepper and dried ginger, and the mass ratio of cloves, gallic acid, pepper and dried ginger is 9:12:8:16;

[0054] The mass ratio of camel hump compound ointment to lavender essential oil is 290:1;

[0055] The preparation method of the camel hump composite ointment comprises the following steps:

[0056] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 85°C for 7 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0057] 2) 90 g of cloves, 120 g of gallic acid, 80 g of Zanthoxylum bungeanum, and 160 g of dried ginger were taken; the above ingredients were crushed separately and passed through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing according to the proportion, ethanol was added for extraction, the ethanol extraction was performed twice, and the extract was concentrated under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration was 0.91; the mass concentration of ethanol used in the two ethanol extractions was 35%; the amount of ethanol added in the two extractions was 10:1 to the total mass of cloves, gallic acid, Zanthoxylum bungeanum, and dried ginger;

[0058] 3) 450 g of the camel hump oil obtained in step 1) was heated to 65° C., and 25 g of egg butter, 80 g of emu oil, 50 g of beeswax, 15 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 3.5 h, cooled and aliquoted to obtain a camel hump composite ointment.

[0059] Example 2

[0060] Camel hump composite ointment comprises the following raw materials in parts by weight:

[0061] 680g camel hump oil, 85g egg butter, 295g emu oil, 250g beeswax, 95g Chinese herbal extract, lavender essential oil;

[0062] The Chinese herbal medicine extract includes cloves, gallic acid, pepper and dried ginger, and the mass ratio of cloves, gallic acid, pepper and dried ginger is 15:23:32:48;

[0063] The mass ratio of camel hump compound ointment to lavender essential oil is 428:1;

[0064] The preparation method of the camel hump composite ointment comprises the following steps:

[0065] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 150°C for 3 hours. Then filter to remove impurities and take the camel hump oil for later use;

[0066] 2) Taking 150g of cloves, 23g of gallic acid fruit, 32g of Sichuan peppercorns, and 48g of dried ginger; crushing the above ingredients separately and passing through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; mixing them in proportion, adding ethanol for extraction, performing ethanol extraction twice, and concentrating under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 1.25; the mass concentration of ethanol used in both ethanol extractions is 85%; the amount of ethanol added in both extractions is 28:1 to the total mass of cloves, gallic acid fruit, Sichuan peppercorns, and dried ginger;

[0067] 3) 680 g of the camel hump oil obtained in step 1) was heated to 135° C., and 85 g of egg butter, 295 g of emu oil, 250 g of beeswax, 95 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 1.1 h, cooled and aliquoted to obtain a camel hump composite ointment.

[0068] Example 3

[0069] Camel hump composite ointment comprises the following raw materials in parts by weight:

[0070] 500g camel hump oil, 50g egg butter, 150g emu oil, 100g beeswax, 40g Chinese herbal extract, lavender essential oil;

[0071] The Chinese herbal medicine extract includes cloves, gallic acid, Zanthoxylum bungeanum and dried ginger, and the mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is 10:15:15:25;

[0072] The mass ratio of camel hump compound ointment to lavender essential oil is 320:1;

[0073] The preparation method of the camel hump composite ointment comprises the following steps:

[0074] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 100°C for 5 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0075] 2) Take 100g of cloves, 150g of gallic acid, 150g of Sichuan peppercorns, and 250g of dried ginger; crush the above ingredients separately and pass them through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing them in proportion, add ethanol for extraction, perform ethanol extraction twice, and concentrate under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 1; the mass concentration of ethanol used in the two ethanol extractions is 50%; the amount of ethanol added in the two extractions is 15:1 to the total mass of cloves, gallic acid, Sichuan peppercorns, and dried ginger;

[0076] 3) 500 g of the camel hump oil obtained in step 1) was heated to 80° C., and then 50 g of egg butter, 150 g of emu oil, 100 g of beeswax, 40 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 2 h, cooled and aliquoted to obtain a camel hump composite ointment.

[0077] Example 4

[0078] Camel hump composite ointment comprises the following raw materials in parts by weight:

[0079] 600g camel hump oil, 70g egg butter, 250g emu oil, 200g beeswax, 70g Chinese herbal extract, lavender essential oil;

[0080] The Chinese herbal medicine extract includes cloves, gallic acid, Zanthoxylum bungeanum and dried ginger, and the mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is 13:20:20:35;

[0081] The mass ratio of camel hump compound ointment to lavender essential oil is 400:1;

[0082] The preparation method of the camel hump composite ointment comprises the following steps:

[0083] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 120°C for 5 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0084] 2) Taking 130g of cloves, 200g of gallic acid, 200g of Sichuan peppercorns, and 350g of dried ginger; crushing the above ingredients separately and passing through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; mixing them in proportion, adding ethanol for extraction, extracting with ethanol twice, and concentrating under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 0.91-1.25; the mass concentration of ethanol used in both ethanol extractions is 70%; the amount of ethanol added in both extractions is 25:1 to the total mass of cloves, gallic acid, Sichuan peppercorns, and dried ginger;

[0085] 3) 600 g of camel hump oil obtained in step 1) was heated to 100° C., and 70 g of egg butter, 250 g of emu oil, 200 g of beeswax, 70 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 3 h, cooled and aliquoted to obtain a camel hump composite ointment.

[0086] Example 5

[0087] Camel hump composite ointment comprises the following raw materials in parts by weight:

[0088] 550g camel hump oil, 60g egg butter, 200g emu oil, 150g beeswax, 60g Chinese herbal extract, lavender essential oil;

[0089] The Chinese herbal medicine extract includes cloves, gallic acid, Zanthoxylum bungeanum and dried ginger, and the mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is 12:18:18:30;

[0090] The mass ratio of camel hump compound ointment to lavender essential oil is 350:1;

[0091] The preparation method of the camel hump composite ointment comprises the following steps:

[0092] 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 85-150°C for 3-7 hours. Then filter to remove impurities and take the camel hump oil for later use.

[0093] 2) Take 120g of cloves, 180g of gallic acid, 180g of Sichuan peppercorns, and 300g of dried ginger; grind the above ingredients separately and pass them through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing them in proportion, add ethanol for extraction, perform ethanol extraction twice, and concentrate under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 1.1; the mass concentration of ethanol used in both ethanol extractions is 60%; the amount of ethanol added in both extractions is 19:1 to the total mass of cloves, gallic acid, Sichuan peppercorns, and dried ginger;

[0094] 3) 550 g of the camel hump oil obtained in step 1) was heated to 100° C., and 60 g of egg butter, 200 g of emu oil, 150 g of beeswax, 40 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 2.5 h, cooled and aliquoted to obtain a camel hump composite ointment.

[0095] The following experiments were conducted using the camel hump composite ointment prepared in Example 5 as an example.

[0096] 1) Acute Toxicity Test: Sixty SD rats, half male and half female, were randomly divided into a blank group, experimental group 1 (intact skin, low-dose Camel Hump composite ointment), experimental group 2 (intact skin, high-dose Camel Hump composite ointment), experimental group 3 (damaged skin, low-dose Camel Hump composite ointment), and experimental group 4 (damaged skin, high-dose Camel Hump composite ointment). Hair was removed and the Camel Hump composite ointment prepared in Example 5 was applied externally for 15 consecutive days. Results: No rats died in either the intact or damaged skin groups. The rats in each group moved freely, had normal feeding, defecation, urination, and mental state. There was no inflammatory reaction such as redness, swelling, or bleeding at the damaged skin site. No systemic toxicity or side effects were observed, indicating that the Camel Hump composite ointment prepared by the present invention has no acute toxicity and is safe for use.

[0097] 2) Skin irritation test: The camel hump composite ointment prepared by the present invention was non-irritating to the intact skin of rats; it was less irritating to the damaged skin, and no local skin irritation phenomena such as erythema, swelling, infection, and exudation were observed.

[0098] 3) Allergy test: 30 rats, half male and half female, were randomly divided into groups using a random number table. Results: After application of the camel hump composite ointment prepared by the present invention, the rats showed no abnormalities in coat color or mental state, indicating that the camel hump composite ointment prepared by the present invention does not cause allergic reactions.

[0099] 4) Long-term toxicity experiment: Rats were treated with the drug for 60 consecutive days. Results: After using the Camel Hump composite ointment prepared by the present invention, the rats showed no abnormalities in coat color, activity, urination and defecation, body weight, organ weight, blood count, blood sugar, blood lipids, liver and kidney function, etc. Histomorphological observations showed that no histological changes were observed in any of the rat organs after 60 days of treatment and 2 weeks after drug withdrawal. During the treatment period and after drug withdrawal, the rats in each group showed no abnormalities, moved freely, ate normally, and had no abnormal urination and defecation. This indicates that the Camel Hump composite ointment prepared by the present invention has no long-term toxicity and is safe for use.

[0100] 5) Expression of Inflammatory Factors: Forty 8-week-old SD rats were randomly divided into a blank group and a modeling group. A knee osteoarthritis model was established in the modeling group using bilateral intra-articular injection of 4% papain. The rats were then randomly divided into a model group and an experimental group. The blank group and the model group were fed a standard diet. The experimental group was topically treated with camel hump compound oil twice daily for 12 weeks. The expression of inflammatory factors IL-1β, IL-6, and TNF-α in the synovium was assessed using the ELIASE method. The results are shown in Table 1.

[0101] Table 1 Changes in the expression of inflammatory factors in the synovium of knee osteoarthritis after treatment

[0102] IL-1β (pg / mL) IL-6 (pg / mL) TNF-α (pg / mL) Blank group 9.13±0.86 8.71±0.69 9.19±0.87 Model Group 16.28±0.56 17.09±0.48 18.86±0.65 Experimental group 10.96±0.72 11.89±0.89 13.71±0.58

[0103] 6) Clinical Symptoms: 48 patients with knee osteoarthritis (21 males and 27 females) received topical application of Camel Hump Compound Ointment twice daily for 12 weeks. Knee joint motor function, WOMAC index, and clinical efficacy were observed before and after treatment. The results are shown in Tables 2-4.

[0104] Table 2 Comparison of knee joint motor function scores before and after treatment

[0105] Number of cases Before treatment After treatment Experimental group 48 70.82±10.57 90.51±6.93

[0106] Table 3 Comparison of WOMAC index of knee joint before and after treatment

[0107] Number of cases Before treatment After treatment Experimental group 48 55.16±10.35 25.45±7.05

[0108] Table 4 Clinical efficacy after treatment of knee osteoarthritis

[0109]

[0110]

[0111] 7) Typical Cases

[0112] Patient, Muhebuli Abulizi, 8 years ago (age was 45 years old at the time, body weight 82 kilograms) started double knee joints mild pain, pain was obvious when walking. Later in France Paris, during study half a year, because it rained a lot, weather changeable, pain obviously aggravated, went to hospital for examination and was diagnosed as rheumatoid arthritis, osteoarthritis began mild deformation (X-ray film), plasma inflammatory factor obviously exceeded the standard, sometimes bilateral osteoarthritis was painful when walking, therefore started to take medicine, i.e. oral ibuprofen sustained-release capsules twice each time one and some Chinese patent medicines every day, affected part was smeared with Voltaren cream, pain was alleviated, but pain still continued, especially aggravated when going up and down subway stairs, and was immobile, so continued for two years. Adopt the Camelback composite ointment obtained by embodiment 5 afterwards, rub and touch on knees every day, and bask in the sun for about 1 hour. On the sixth day of this medication, the pain began to subside significantly. On the 12th day, the pain was essentially gone. I continued taking the medication for three weeks and stopped taking ibuprofen and other Western medications. There was no recurrence, and I then stopped using the lotion (for a total of five weeks). It has now been eight years and there has been no recurrence.

[0113] During this process, the technical solution of this application received project funding from the Xinjiang Medical University College Student Innovation Training Program. Because the ingredients contained in the preparations of this project are all highly safe natural medicinal materials and camel hump oil, after safety testing, they were tried on volunteers with osteoarthritis to collect preliminary clinical epidemiological data, and good satisfaction was achieved.

[0114] Many shortcomings were found when the ointment was prepared according to the old process, such as: (1) Camel hump oil has a strong fishy smell, which makes social communication inconvenient after use. Therefore, some volunteers reported that they were forced to wash the medicine after use, which may affect the efficacy; (2) When the ointment was prepared according to the old process, the mixture of medicinal materials and oil was exposed to the sun for 15 days, and the preparation cycle was very long; (3) After the ointment prepared by the old process was applied to the affected area and exposed to the sun, a lot of unabsorbed powder was left on the skin; (4) Camel hump oil is very oily and is not completely absorbed after 1 hour of exposure to the sun. If less ointment is applied, the efficacy will not be significant.

[0115] While retaining the original characteristics of the formula, this application extracts the medicinal materials with aqueous ethanol and concentrates them to a certain ratio (both to ensure the effective components are fully extracted and to produce an extract with better absorption properties). The extract is then mixed with camel hump oil (mixing temperature and stirring time are also technically critical). A certain amount of emu oil is added as a transdermal absorption enhancer (a special precious oil extracted from the fat sack on the back of the emu, an ostrich species. It is more effective and safer than chemical transdermal absorption enhancers, and emu oil itself also has anti-inflammatory properties). A certain amount of beeswax is added as a setting agent, and the subsequent technical measures described in the patented process are implemented. This preparation prepared according to the new process has significant advantages over the existing preparations in many aspects, including improved drugability, stability, therapeutic efficacy, transdermal absorption characteristics, improved odor, and beautiful color, laying an important foundation for the transformation and industrialization of the results of this scientific and technological innovation.

[0116] The above-described cases and comparisons of the features of the new and old processes are provided to the extent that they enable those skilled in the art to implement or use the disclosed embodiments. The above description of the disclosed embodiments will enable those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A camel hump composite ointment, characterized in that: It is prepared from the following raw materials in parts by weight: 550g camel hump oil, 60g egg butter, 200g emu oil, 150g beeswax, 60g Chinese herbal extract, lavender essential oil; The Chinese herbal medicine extract includes cloves, gallic acid, Zanthoxylum bungeanum and dried ginger, and the mass ratio of cloves, gallic acid, Zanthoxylum bungeanum and dried ginger is 12:18:18:30; The mass ratio of camel hump compound ointment to lavender essential oil is 350:1; The preparation method of the camel hump composite ointment comprises the following steps: 1) Cut fresh or frozen camel hump into small pieces, place in a pot, and heat at 85-150°C for 3-7 hours. Then filter to remove impurities and take the camel hump oil for later use. 2) Take 120g of cloves, 180g of gallic acid, 180g of Sichuan peppercorns, and 300g of dried ginger; grind the above ingredients separately and pass them through a 20-mesh sieve to obtain a fine Chinese herbal medicine powder; after mixing them in proportion, add ethanol for extraction, perform ethanol extraction twice, and concentrate under reduced pressure to obtain a Chinese herbal medicine extract; the relative density of the Chinese herbal medicine extract after reduced pressure concentration is 1.1; the mass concentration of ethanol used in both ethanol extractions is 60%; the amount of ethanol added in both extractions is 19:1 to the total mass of cloves, gallic acid, Sichuan peppercorns, and dried ginger; 3) 550 g of the camel hump oil obtained in step 1) was heated to 100° C., and 60 g of egg butter, 200 g of emu oil, 150 g of beeswax, 40 g of the Chinese herbal medicine extract obtained in step 2) and lavender essential oil were added. The mixture was heated with stirring for 2.5 h, cooled and aliquoted to obtain a camel hump composite ointment.

2. Use of the camel hump composite ointment according to claim 1 in preparing a medicine for treating knee osteoarthritis.

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