A multi-flux cell extraction device

Abstract

The present invention relates to a multi-flux cell extraction device, which includes a base, a first bracket, which is arranged on the base, and the first bracket is provided with two X-axis axial guide rails and one Y-axis axial guide rail; a second bracket, which is provided with a slideway matched with the Y-axis axial guide rail; a container assembly, which is arranged on the base, and the container assembly includes a plurality of sample tube racks; a suction assembly, which is arranged on the base and electrically connected to the circuit control assembly; a pipetting assembly, which is arranged on the second bracket and connected to the suction assembly through a conduit, and the pipetting assembly is electrically connected to the circuit control assembly; a capping extraction assembly, which is arranged on the second bracket and electrically connected to the circuit control assembly. The device of the present invention integrates multiple components such as a pipetting assembly, a capping extraction assembly, and a pipette disassembly assembly, and can perform various operations on cell fluid extraction and sample tube management, which greatly improves the operating efficiency.

Description

Technical Field

[0001] The invention relates to a multi-flux cell extraction device, belonging to the technical field of biomedical equipment. Background Art

[0002] In the prior art, the cell extraction process needs to be monitored by humans to determine the height of the cell layer to be extracted and to transfer the cells using a pipette. This operation requires the operator to operate in a biosafety cabinet. As the number of samples increases, the operator needs to frequently open and close the lid, and perform other actions such as aspirating and draining liquids, which can easily lead to unstable extracted cell layers. Frequent operations are labor-intensive and not only easily cause fatigue but also are prone to the risk of operator contamination.

[0003] In addition, existing extraction devices are often single-throughput operations, which are inefficient when multiple samples need to be operated simultaneously. In addition, the screw-capping mechanism has a complex structure, occupies a large space, and is costly. Summary of the invention

[0004] In response to the above-mentioned prominent problems, the present invention provides a multi-throughput cell extraction device. The device of the present invention is equipped with a suction component and a pipette component matching the suction component. The pipette component is provided with a plurality of independently operable pipette clamping parts. It can accurately measure a variety of cell fluids at the same time, avoid operational errors in manual extraction of cell fluids, and improve operational efficiency.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] A multi-flux cell extraction device comprises the following components:

[0007] Base 1,

[0008] A first bracket 2 is arranged on the base 1, and two X-axis axial guide rails 21 and a Y-axis axial guide rail 22 are arranged on the first bracket 2, and two ends of the Y-axis axial guide rail 22 are provided with slide grooves matching with the X-axis axial guide rail 21;

[0009] A second bracket 8, on which a slide groove matching the Y-axis axial guide rail 22 is provided;

[0010] A circuit control component is arranged on the base 1;

[0011] A container assembly 6 is disposed on the base 1, and the container assembly 6 includes a plurality of sample tube racks for accommodating samples;

[0012] A suction component 3 is disposed on the base 1 and is electrically connected to the circuit control component, and the circuit control component controls the suction movement of the suction component 3;

[0013] The pipetting assembly 4 is arranged on the second bracket 8 and connected to the suction assembly 3 through a conduit. The pipetting assembly 4 is electrically connected to the circuit control assembly. The circuit control assembly controls the pipetting assembly 4 to slide along the X-axis axial guide rail 21 and / or the Y-axis axial guide rail 22, so as to make the pipetting assembly 4 extend into the container assembly 6 to suck the sample;

[0014] The capping and extracting assembly 5 is disposed on the second bracket 8 and is electrically connected to the circuit control assembly. The circuit control assembly controls the capping and extracting assembly 5 to cap and / or take and place the sample tube in the container assembly 6 .

[0015] The multi-flux cell extraction device, preferably, the second bracket 8 includes a second bracket upper support plate 81 and a second bracket base 82, and a pipette disassembly assembly 9 is arranged on the lower end surface of the second bracket base 82, which includes a fifth motor 91, a slide plate 92, a base 94 and a fixed plate 93, the fifth motor 91 is fixed on the base 94, the slide plate 92 is arranged above the base 94, and a fixed plate 93 is arranged above the slide plate 92, and the fixed plate 93 is fixed on the lower end surface of the second bracket base 82; a gear 941 is arranged on the base 94, a rack 923 and a disassembly hole 921 are arranged on the slide plate 92, the output end of the fifth motor 91 is connected to the gear 941, and the gear 941 is meshed with the rack 923 to drive the slide plate 92 to extend and retract.

[0016] The multi-throughput cell extraction device, preferably, the pipetting assembly 4 includes a plurality of first transmission shafts 45, a plurality of first motors 44, a plurality of first sliders 41 and a plurality of second sliders 42 arranged in a one-to-one correspondence with the plurality of first sliders 41, the first sliders 41 and the second sliders 42 are detachably fixedly connected, the first sliders 41 are one-to-one ringed on the first transmission shaft 45, the first transmission shaft 45 is connected to the output end of the first motor 44 through a first belt 46, and the lower end of the second slider 42 is provided with a pipette clamping portion 43 for clamping a pipette.

[0017] The multi-throughput cell extraction device preferably also includes a sample liquid level detection component 10, which is arranged on the first slider 41 on one side of the pipetting component 4, and includes a first clamping leg 101 and a second clamping leg 102, and both are parallel to the first transmission shaft 45, and a pair of openings are provided near the lower ends of the first clamping leg 101 and the second clamping leg 102, and a transmitter and a receiver are provided in the openings.

[0018] In the multi-flux cell extraction device, preferably, the screw cap extraction component 5 includes a third bracket 51, and the third bracket 51 includes a third bracket base 511 and a third bracket upper support plate 512;

[0019] A plurality of clamping mechanisms are arranged on the third bracket base 511, and the clamping mechanisms include a fourth motor 59 and a fourth transmission shaft 591, the output end of the fourth motor 59 is connected to the fourth transmission shaft 591, and the fourth transmission shaft 591 is provided with a clamping component for clamping the sample container;

[0020] A plurality of cover-screwing mechanisms are arranged on the third bracket base 511 and are arranged one-to-one corresponding to the plurality of clamping mechanisms. The cover-screwing mechanisms include a third motor 53 and a third transmission shaft 531. The output end of the third motor 53 is connected to the third transmission shaft 531. The third transmission shaft 531 is connected to the gear sleeved on the fourth transmission shaft 591 through a second belt 56.

[0021] The lifting mechanism is arranged on the support plate 512 on the third bracket, and the lifting mechanism includes a second motor 52 and a second transmission shaft 521. The output end of the second motor 52 is connected to the second transmission shaft 521, and the lower end of the second transmission shaft 521 is connected to the support plate 512 on the third bracket, and is used to drive the several clamping structures and the several rotating cover mechanisms to rise or fall.

[0022] The multi-flux cell extraction device, preferably, the sample tube rack includes a container bottom plate 62, a container support frame 61, a container side plate 64 and a container upper cover plate 63, the container bottom plate 62 is provided with a plurality of fixing columns 621 and a plurality of rows of second tube holes 622, the container upper cover plate 63 is provided with fixing holes 631 and first tube holes 632 corresponding to the fixing columns 621 and the second tube holes 622, respectively, and the container support frame 61 is provided with two container support frame side plates 614, one end of the two container support frame side plates 614 Each of them is provided with an adjustable wrench 611, and is also provided with a limit hole 613 passing through the upper and lower end surfaces thereof, and a limit pin is provided in the limit hole 613; a plurality of rows of protective covers 612 corresponding to the first tube hole 632 are provided between the two container support frame side plates 614; the container support frame 61 is sleeved on the outer side of the fixing column 621, the container side plate 64 cooperates with the container support frame 61, the upper end of the container side plate 64 is covered with the container upper cover plate 63, and the free end of the fixing column 621 is fixed in the fixing hole 631.

[0023] The multi-flux cell extraction device described above, preferably, the suction assembly 3 includes a fourth bracket 37, a suction cylinder 34, a sixth transmission shaft 32 and a sixth motor 31, the fourth bracket 37 includes a fourth bracket horizontal support plate 371 and a fourth bracket vertical support plate 372, the sixth motor 31 is fixed on the lower end surface of the fourth bracket horizontal support plate 371, the output end of the sixth motor 31 is connected to the sixth transmission shaft 32, one end of the suction cylinder 34 is fixed to the fourth bracket vertical support plate 372 through a suction cylinder fixing column 35, and the other end thereof is connected to the sixth transmission shaft 32 through a screw rod and a third belt 33.

[0024] In the multi-flux cell extraction device, preferably, the clamping component includes a third slider 54 and three mechanical claws 55, the third slider 54 is a hollow cylindrical body, and the cavity of the third slider 54 is used to accommodate the fourth transmission shaft 591; the three mechanical claws 55 are evenly arranged on the outer side wall of the third slider 54; and / or,

[0025] The mechanical claw 55 is an ear-shaped structure, which includes a first clamping portion 551 and a second clamping portion 552. The second clamping portion 552 is arranged directly below the first clamping portion 551. The first clamping portion 551 is provided with a first clamping surface 555, and the second clamping portion 552 is provided with a second clamping surface 556. The first clamping surface 555 and the second clamping surface 556 are respectively used to clamp sample container covers of different sizes.

[0026] In the multi-flux cell extraction device, preferably, the first clamping surface 555 and the second clamping surface 556 are respectively provided with ridges 554 for clamping the sample container cover, and the lower end of the second clamping portion 552 is provided with a step 557 protruding toward the axis for clamping the sample container body; and / or,

[0027] The first clamping portion 551 is provided with an eyelet hole 553 for fixing the mechanical claw 55 on the outer side wall of the third sliding block 54 .

[0028] In the multi-flux cell extraction device, preferably, the telescopic end of the slide plate 92 is provided with a groove 922 for receiving the leakage of the pipette.

[0029] The present invention adopts the above technical solution, which has the following advantages:

[0030] 1. The device of the present invention is provided with a suction component and a liquid transfer component matched with the suction component. The liquid transfer component is provided with a plurality of independently operable liquid transfer tube clamping parts, which can accurately measure a plurality of cell fluids at the same time, avoid the operation error existing in manual extraction of cell fluids, and improve the operation efficiency at the same time;

[0031] 2. The capping and extraction assembly of the present invention is provided with a plurality of independently operable clamping mechanisms and a capping mechanism matched with the clamping mechanisms, which can simultaneously open, close and extract a plurality of sample tubes, thereby greatly improving the efficiency of capping and extracting the sample tubes;

[0032] 3. The present invention can monitor the height of the cell liquid level in real time and accurately measure the cell liquid by arranging a sample liquid level detection component on the liquid transfer component, and arranging an optical fiber and an optical fiber signal transmitter and receiver in the component;

[0033] 4. The present invention provides a pipette disassembly assembly at the lower end of the pipette assembly, so that the discarded pipette can be quickly disassembled from the device without manual replacement of the pipette, thereby improving work efficiency;

[0034] 5. The sample tube rack of the present invention is provided with a container support frame, and a sample tube protective cover, a limit hole, a limit pin and a wrench are provided on the container support frame. The protective cover is controlled to clamp or loosen the sample tube by pulling the wrench to avoid shaking of the sample tube during the liquid collection process, resulting in inaccurate liquid collection amount;

[0035] 6. The present invention forms a stable triangular structure when grabbing a sample container by arranging three evenly distributed mechanical claws on the outer side wall of the third slider. The three mechanical claws clamp the sample container cover at the same time, with high concentricity and strong stability in the rotation switch action. On the other hand, two clamping parts are arranged on the mechanical claw, and the clamping angles of the clamping surfaces of the two clamping parts are different, so that sample container covers of different sizes can be clamped. On the other hand, convex ridges are arranged on the first clamping surface and the second clamping surface, so that when the cover is screwed, the convex ridges match the threads on the sample container cover, thereby enhancing the force between the clamping part and the screw cover, which is conducive to opening and closing the cover. A step is arranged at the lower end of the second clamping part, so that the sample container cover can be quickly opened.

[0036] 7. The motor in the present invention is driven by a pulley, and the torque is amplified by the gear ratio of the master and driven wheels, so that a large rotational force can be exerted under a very small motor, and the phenomenon of being unable to be unscrewed will not occur during the process of screwing the cap;

[0037] 8. The device of the present invention integrates multiple components such as a pipetting component, a screw-cap extraction component, and a pipette disassembly component, and can perform various operations on cell fluid extraction and sample tube management, truly realizing the diversification of cell fluid in vitro operations and greatly improving operating efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the overall structure of the device of the present invention;

[0039] Figure 2 It is a schematic diagram of the structure of the liquid transfer component in the device of the present invention;

[0040] Figure 3 It is a schematic diagram of the structure of the liquid transfer component in the device of the present invention when it is in operation;

[0041] Figure 4 It is a structural schematic diagram of the capping and extracting component in the device of the present invention;

[0042] Figure 5 It is a partial structural schematic diagram of the capping and extracting component in the device of the present invention;

[0043] Figure 6 It is a structural schematic diagram of the pipette disassembly assembly in the device of the present invention;

[0044] Figure 7 It is a partial structural schematic diagram of the pipette disassembly assembly in the device of the present invention;

[0045] Figure 8 It is a schematic diagram of the structure of the suction assembly in the device of the present invention;

[0046] Fig. 9 It is a schematic diagram of the structure of the container assembly in the device of the present invention;

[0047] Fig.10 for Fig. 9 Exploded view of the container component in the figure;

[0048] Fig.11 It is a schematic diagram of the structure of the sample liquid level detection component in the device of the present invention;

[0049] Fig.12 It is a schematic diagram of the structure of the mechanical claw in the device of the present invention;

[0050] Fig.13 Another structural schematic diagram of the container assembly in the device of the present invention;

[0051] Fig.14 for Fig.13 Exploded view of the container component in the figure;

[0052] The marks in the figure are as follows:

[0053] 1-base; 2-first bracket, 21-X-axis axial guide rail, 22-Y-axis axial guide rail; 3-suction assembly, 31-sixth motor, 32-sixth transmission shaft, 33-third belt, 34-suction cylinder, 35-suction cylinder fixing column, 36-catheter accommodating hole, 37-fourth bracket, 371-fourth bracket horizontal support plate, 372-fourth bracket vertical support plate; 4-pipette assembly, 41-first slider, 42-second slider, 43-pipette clamping part, 44-first a motor, 45-a first transmission shaft, 46-a first belt; 5-a cap extraction assembly, 51-a third bracket, 511-a third bracket base, 512-a third bracket upper support plate, 52-a second motor, 521-a second transmission shaft, 53-a third motor, 531-a third transmission shaft, 54-a third slider, 55-a mechanical claw, 551-a first clamping portion, 552-a second clamping portion, 553-an ear hole, 554-a convex ridge, 555-a first clamping surface, 556-a third Second clamping surface, 557-step, 56-second belt, 57-first horizontal fixing plate, 58-support rod, 59-fourth motor, 591-fourth transmission shaft; 6-container assembly, 61-container support frame, 611-wrench, 612-protective cover, 613-limiting hole, 614-container support frame side plate, 62-container bottom plate, 621-fixing column, 622-second pipe hole, 623-protrusion, 63-container upper cover, 631-fixing hole, 632-first pipe hole, 64-container side plate; 7-display assembly; 8-second bracket, 81-second bracket upper support plate, 82-second bracket base; 9-pipette disassembly assembly, 91-fifth motor, 92-slide plate, 921-disassembly hole, 922-groove, 923-rack, 93-upper fixed plate, 94-base, 941-gear; 10-sample liquid level detection assembly, 101-first clamping leg, 102-second clamping leg, 103-optical fiber box; 11-gun tip box. DETAILED DESCRIPTION

[0054] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary persons in the art without creative work are within the scope of protection of the present invention.

[0055] Example 1

[0056] like Figures 1 to 4 As shown, this embodiment relates to a multi-flux cell extraction device, comprising the following components:

[0057] Base 1,

[0058] A first bracket 2 is arranged on the base 1, and two X-axis axial guide rails 21 and one Y-axis axial guide rail 22 are arranged on the first bracket 2, and two ends of the Y-axis axial guide rail 22 are provided with slide grooves matching with the X-axis axial guide rail 21;

[0059] A second bracket 8, on which a slide groove matching the Y-axis axial guide rail 22 is provided;

[0060] A circuit control component is arranged on the base 1;

[0061] A container assembly 6 is disposed on the base 1, and the container assembly 6 includes a plurality of sample tube racks for accommodating samples;

[0062] A suction component 3 is disposed on the base 1 and is electrically connected to the circuit control component, and the circuit control component controls the suction movement of the suction component 3;

[0063] The pipetting assembly 4 is arranged on the second bracket 8 and connected to the suction assembly 3 through a conduit. The pipetting assembly 4 is electrically connected to the circuit control assembly. The circuit control assembly controls the pipetting assembly 4 to slide along the X-axis axial guide rail 21 and / or the Y-axis axial guide rail 22, so as to make the pipetting assembly 4 extend into the container assembly 6 to suck the sample;

[0064] The capping and extracting assembly 5 is disposed on the second bracket 8 and is electrically connected to the circuit control assembly. The circuit control assembly controls the capping and extracting assembly 5 to cap and / or take and place the sample tube in the container assembly 6 .

[0065] In this embodiment, preferably, a display component 7 is further included, which is used to display the extraction status of the cell fluid and control the operation of the pipetting component 4 and the capping extraction component 5.

[0066] In this embodiment, preferably, the second bracket 8 includes a second bracket upper support plate 81 and a second bracket base 82, and a pipette disassembly assembly 9 is arranged on the lower end surface of the second bracket base 82, which includes a fifth motor 91, a slide plate 92, a base 94 and a fixed plate 93, the fifth motor 91 is fixed on the base 94, the slide plate 92 is arranged above the base 94, and a fixed plate 93 is arranged above the slide plate 92, and the fixed plate 93 is fixed on the lower end surface of the second bracket base 82; a gear 941 is arranged on the base 94, a rack 923 and a disassembly hole 921 are arranged on the slide plate 92, and the output end of the fifth motor 91 is connected to the gear 941, and the gear 941 is meshed with the rack 923 to drive the slide plate 92 to extend and retract.

[0067] In this embodiment, preferably, the pipetting assembly 4 includes four first transmission shafts 45, four first motors 44, four first sliders 41 and four second sliders 42 arranged in a one-to-one correspondence with the four first sliders 41, the first sliders 41 and the second sliders 42 are detachably fixedly connected, the first sliders 41 are ringed on the first transmission shafts 45 in a one-to-one correspondence, the first transmission shaft 45 is connected to the output end of the first motor 44 through a first belt 46, and the lower end of the second slider 42 is provided with a pipette clamping portion 43 for clamping a pipette.

[0068] In this embodiment, preferably, it also includes a sample liquid level height detection component 10, which is arranged on the first slider 41 on one side of the pipetting component 4, and includes a first clamping leg 101 and a second clamping leg 102, and both are parallel to the first transmission shaft 45, and a pair of openings are provided near the lower ends of the first clamping leg 101 and the second clamping leg 102, and a transmitter and a receiver are provided in the openings.

[0069] In this embodiment, preferably, the capping and extracting assembly 5 comprises a third bracket 51, and the third bracket 51 comprises a third bracket base 511 and a third bracket upper support plate 512;

[0070] Four clamping mechanisms are arranged on the third bracket base 511, and the clamping mechanisms include a fourth motor 59 and a fourth transmission shaft 591. The output end of the fourth motor 59 is connected to the fourth transmission shaft 591, and the fourth transmission shaft 591 is provided with a clamping component for clamping the sample container;

[0071] Four capping mechanisms are arranged on the third bracket base 511 and are arranged one-to-one corresponding to the four clamping mechanisms. The capping mechanisms include a third motor 53 and a third transmission shaft 531. The output end of the third motor 53 is connected to the third transmission shaft 531. The third transmission shaft 531 is connected to the gear sleeved on the fourth transmission shaft 591 through a second belt 56.

[0072] A lifting mechanism is arranged on the support plate 512 on the third bracket, and the lifting mechanism includes a second motor 52 and a second transmission shaft 521. The output end of the second motor 52 is connected to the second transmission shaft 521, and the lower end of the second transmission shaft 521 is connected to the support plate 512 on the third bracket, and is used to drive the four clamping structures and the four rotating cover mechanisms to rise or fall.

[0073] In this embodiment, preferably, the sample tube rack includes a container bottom plate 62, a container support frame 61, a container side plate 64 and a container upper cover plate 63, the container bottom plate 62 is provided with four fixing columns 621 and four rows of second tube holes 622, the container upper cover plate 63 is provided with fixing holes 631 and first tube holes 632 corresponding to the fixing columns 621 and the second tube holes 622, respectively, and the container support frame 61 is provided with two container support frame side plates 614, and one end of each of the two container support frame side plates 614 is provided with An adjustable wrench 611 is also provided with a limit hole 613 passing through its upper and lower end surfaces, and a limit pin is provided in the limit hole 613; four rows of protective covers 612 corresponding to the first tube holes 632 are provided between the two container support frame side plates 614; the container support frame 61 is sleeved on the outer side of the fixing column 621, the container side plate 64 cooperates with the container support frame 61, the upper end of the container side plate 64 is covered with the container upper cover plate 63, and the free end of the fixing column 621 is fixed in the fixing hole 631.

[0074] In this embodiment, preferably, the suction assembly 3 includes a fourth bracket 37, a suction cylinder 34, a sixth transmission shaft 32 and a sixth motor 31, the fourth bracket 37 includes a fourth bracket horizontal support plate 371 and a fourth bracket vertical support plate 372, the sixth motor 31 is fixed on the lower end surface of the fourth bracket horizontal support plate 371, the output end of the sixth motor 31 is connected to the sixth transmission shaft 32, one end of the suction cylinder 34 is fixed to the fourth bracket vertical support plate 372 through a suction cylinder fixing column 35, and the other end thereof is connected to the sixth transmission shaft 32 through a screw rod and a third belt 33.

[0075] In this embodiment, preferably, the clamping component includes a third slider 54 and three mechanical claws 55, the third slider 54 is a hollow cylindrical shape, and the cavity of the third slider 54 is used to accommodate the fourth transmission shaft 591; the three mechanical claws 55 are evenly arranged on the outer wall of the third slider 54.

[0076] In this embodiment, preferably, the mechanical claw 55 is an ear-shaped structure, which includes a first clamping portion 551 and a second clamping portion 552, the second clamping portion 552 is arranged directly below the first clamping portion 551, the first clamping portion 551 is provided with a first clamping surface 555, and the second clamping portion 552 is provided with a second clamping surface 556, the first clamping surface 555 and the second clamping surface 556 are respectively used to clamp sample container covers of different sizes.

[0077] In this embodiment, preferably, the first clamping surface 555 and the second clamping surface 556 are respectively provided with ridges 554 for clamping the sample container cover, and the lower end of the second clamping portion 552 is provided with a step 557 protruding toward the axis for clamping the sample container body.

[0078] In this embodiment, preferably, the first clamping portion 551 is provided with an eyelet hole 553 for fixing the mechanical claw 55 on the outer side wall of the third sliding block 54 .

[0079] In this embodiment, preferably, the telescopic end of the slide plate 92 is provided with a groove 922 for receiving the leakage of the pipette.

[0080] In this embodiment, preferably, the sample liquid level detection assembly 10 further includes an optical fiber box 103 for accommodating redundant optical fibers.

[0081] In this embodiment, preferably, the device of the present invention further comprises a tip box 11, wherein the number of the tip boxes 11 is 2 and used for accommodating discarded pipette tips and new pipette tips.

[0082] In this embodiment, preferably, the outside of the catheter connecting the suction component 3 and the pipetting component 4 is wrapped with a hard shell to prevent the problem of inaccurate pipetting amount caused by shaking of the catheter during the pipetting process.

[0083] In this embodiment, preferably, a main motor for driving the X-axis axial guide rail 21 and the Y-axis axial guide rail 22 is also provided.

[0084] Example 2

[0085] This embodiment provides an operating method of the device described in Embodiment 1, including the following steps:

[0086] (1) starting the main motor so that the second bracket 8 slides on the X-axis axial guide rail 21 and / or the Y-axis axial guide rail 22 to the top of the sample tube rack, and then starting the second motor 52, so that the capping mechanism and the clamping mechanism move downward under the drive of the second motor 52;

[0087] (2) When the capping mechanism and the clamping mechanism reach a specified height, the second motor 52 is turned off, and the fourth motor 59 and the third motor 53 are turned on. Driven by the fourth motor 59 and the third motor 53, the mechanical claw 55 clamps the sample container body and unscrews the sample container cap;

[0088] (3) Turning on the first motor 44 and the sixth motor 31 to extract the solution in the sample container, during the sampling process, the liquid level in the sample container is monitored in real time by the sample liquid level monitoring component 10 to achieve accurate measurement;

[0089] (4) When the sampling operation is completed, the circuit control component controls the first motor 44 to drive the pipette disassembly component 9 to disassemble the discarded pipette, and put the discarded pipette into the gun tip box 11 to complete the operation.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-throughput cell extraction device, It is characterized in that Includes the following components: Base (1), A first bracket (2) is arranged on the base (1), and two X-axis axial guide rails (21) and a Y-axis axial guide rail (22) are arranged on the first bracket (2), and two ends of the Y-axis axial guide rail (22) are provided with sliding grooves that match the X-axis axial guide rail (21); A second bracket (8) having a slide groove matched with the Y-axis axial guide rail (22); A circuit control component is arranged on the base (1); A container assembly (6) is arranged on the base (1), and the container assembly (6) comprises a plurality of sample tube racks for accommodating samples; A suction component (3) is arranged on the base (1) and is electrically connected to the circuit control component, wherein the circuit control component controls the suction movement of the suction component (3); A pipetting assembly (4) is arranged on the second bracket (8) and connected to the suction assembly (3) via a conduit. The pipetting assembly (4) is electrically connected to the circuit control assembly. The circuit control assembly controls the pipetting assembly (4) to slide along the X-axis axial guide rail (21) and / or the Y-axis axial guide rail (22) so as to allow the pipetting assembly (4) to extend into the container assembly (6) to suction a sample. a capping and extracting assembly (5) disposed on the second bracket (8) and electrically connected to the circuit control assembly, wherein the circuit control assembly controls the capping and extracting assembly (5) to cap and / or remove the sample tube in the container assembly (6); The second bracket (8) comprises a second bracket upper support plate (81) and a second bracket base (82); a pipette disassembly assembly (9) is arranged on the lower end surface of the second bracket base (82), and comprises a fifth motor (91), a slide plate (92), a base (94) and a fixing plate (93); the fifth motor (91) is fixed on the base (94); the slide plate (92) is arranged above the base (94); the fixing plate (93) is arranged above the slide plate (92); the fixing plate (93) is fixed on the lower end surface of the second bracket base (82); a gear (941) is arranged on the base (94); a rack (923) and a disassembly hole (921) are arranged on the slide plate (92); an output end of the fifth motor (91) is connected to the gear (941); the gear (941) meshes with the rack (923) to drive the slide plate (92) to extend and retract; The pipetting assembly (4) comprises a plurality of first transmission shafts (45), a plurality of first motors (44), a plurality of first sliders (41) and a plurality of second sliders (42) arranged in a one-to-one correspondence with the plurality of first sliders (41); the first sliders (41) and the second sliders (42) are detachably fixedly connected; the first sliders (41) are respectively sleeved on the first transmission shafts (45); the first transmission shafts (45) are connected to the output end of the first motor (44) via a first belt (46); and a pipette clamping portion (43) is arranged at the lower end of the second slider (42) for clamping a pipette.

2. The multi-flux cell extraction device according to claim 1, It is characterized in that It also includes a sample liquid level detection component (10), which is arranged on the first slider (41) on one side of the pipetting component (4), and includes a first clamping leg (101) and a second clamping leg (102), and the two are parallel to the first transmission shaft (45), and a pair of openings are arranged near the lower ends of the first clamping leg (101) and the second clamping leg (102), and a transmitter and a receiver are arranged in the openings.

3. The multi-flux cell extraction device according to claim 1, It is characterized in that The capping extraction assembly (5) comprises a third bracket (51), and the third bracket (51) comprises a third bracket base (511) and a third bracket upper support plate (512); a plurality of clamping mechanisms, arranged on the third support base (511), the clamping mechanisms comprising a fourth motor (59) and a fourth transmission shaft (591), the output end of the fourth motor (59) being connected to the fourth transmission shaft (591), and the fourth transmission shaft (591) being provided with a clamping component for clamping a sample container; a plurality of cover-screwing mechanisms, arranged on the third bracket base (511) and arranged one-to-one corresponding to the plurality of clamping mechanisms, the cover-screwing mechanisms comprising a third motor (53) and a third transmission shaft (531), the output end of the third motor (53) being connected to the third transmission shaft (531), and the third transmission shaft (531) being connected to a gear sleeved on the fourth transmission shaft (591) via a second belt (56); A lifting mechanism is arranged on the upper support plate (512) of the third bracket, the lifting mechanism comprising a second motor (52) and a second transmission shaft (521), the output end of the second motor (52) is connected to the second transmission shaft (521), and the lower end of the second transmission shaft (521) is connected to the upper support plate (512) of the third bracket, and is used for driving the plurality of clamping components and the plurality of capping mechanisms to rise or fall.

4. The multi-flux cell extraction device according to claim 1, It is characterized in that The sample tube rack comprises a container bottom plate (62), a container support frame (61), a container side plate (64) and a container upper cover plate (63); the container bottom plate (62) is provided with a plurality of fixing columns (621) and a plurality of rows of second tube holes (622); the container upper cover plate (63) is provided with fixing holes (631) and first tube holes (632) which correspond one-to-one to the fixing columns (621) and the second tube holes (622), respectively; the container support frame (61) is provided with two container support frame side plates (614); one end of each of the two container support frame side plates (614) is provided with an adjustable wrench. (611), and is also provided with a limiting hole (613) penetrating the upper and lower end surfaces thereof, and a limiting pin is provided in the limiting hole (613); a plurality of rows of protective sleeves (612) corresponding to the first tube hole (632) are provided between the two container support frame side plates (614); the container support frame (61) is sleeved on the outer side of the fixing column (621), the container side plate (64) cooperates with the container support frame (61), the upper end of the container side plate (64) is covered with the container upper cover plate (63), and the free end of the fixing column (621) is fixed in the fixing hole (631).

5. The multi-flux cell extraction device according to claim 1, It is characterized in that The suction assembly (3) comprises a fourth bracket (37), a suction cylinder (34), a sixth transmission shaft (32) and a sixth motor (31); the fourth bracket (37) comprises a fourth bracket horizontal support plate (371) and a fourth bracket vertical support plate (372); the sixth motor (31) is fixed to the lower end surface of the fourth bracket horizontal support plate (371); an output end of the sixth motor (31) is connected to the sixth transmission shaft (32); one end of the suction cylinder (34) is fixed to the fourth bracket vertical support plate (372) via a suction cylinder fixing column (35); and the other end thereof is connected to the sixth transmission shaft (32) via a screw rod and a third belt (33).

6. The multi-flux cell extraction device according to claim 3, It is characterized in that The clamping component comprises a third slider (54) and three mechanical claws (55); the third slider (54) is a hollow cylindrical body; the cavity of the third slider (54) is used to accommodate the fourth transmission shaft (591); the three mechanical claws (55) are evenly arranged on the outer side wall of the third slider (54); and / or, The mechanical claw (55) is an ear-shaped structure, comprising a first clamping portion (551) and a second clamping portion (552); the second clamping portion (552) is arranged directly below the first clamping portion (551); the first clamping portion (551) is provided with a first clamping surface (555); the second clamping portion (552) is provided with a second clamping surface (556); the first clamping surface (555) and the second clamping surface (556) are respectively used to clamp sample container covers of different sizes.

7. The multi-flux cell extraction device according to claim 6, It is characterized in that The first clamping surface (555) and the second clamping surface (556) are respectively provided with ridges (554) for clamping the sample container cover, and the lower end of the second clamping portion (552) is provided with a step (557) protruding toward the axis for clamping the sample container body; and / or, The first clamping portion (551) is provided with an eyelet hole (553) for fixing the mechanical claw (55) on the outer side wall of the third sliding block (54).

8. The multi-flux cell extraction device according to claim 1, It is characterized in that The telescopic end of the slide plate (92) is provided with a groove (922) for receiving liquid leakage from the pipette.

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