A card-cage type multi-channel dry immunofluorescence detector

Abstract

The present invention discloses a card-storage type multi-channel dry immunofluorescence detector, comprising a stacked card-storage, an intermediate incubation unit, and a continuous incubation unit. The stacked card-storage is provided with an intermediate incubation unit at the rear, and the intermediate incubation unit is provided with a continuous incubation unit at the rear. The present invention can be adapted to both fixed-time incubation and variable-time incubation, and effectively solves the problems of complex control, low reliability, difficulty in realizing high-speed automation, and poor adaptability in the prior art.

Description

Technical Field

[0001] The invention relates to the field of in vitro diagnostic equipment, and in particular to a card-cage type multi-channel dry immunofluorescence detector. Background Art

[0002] Fully automatic immunoassay is based on the immunological reaction of antigen-antibody binding, uses enzyme labels, lanthanide labels or chemiluminescent agents to label antigens and antibodies, and through a series of cascade amplification reactions, links the light signal or electrical signal with the analyte concentration, etc., to analyze the antigen or antibody to be tested in human samples. It is mainly used in hospital laboratories, third-party independent laboratories, blood test centers and other institutions to perform quantitative, semi-quantitative or qualitative detection of the content of each analyte in human body fluids, and to diagnose infectious diseases, tumors, endocrine function, cardiovascular diseases, eugenics, and autoimmune diseases.

[0003] According to the test principle and mode, immunoassay can be generally divided into one-step method, delayed one-step method, two-step method, etc. The main test steps generally include adding samples and reagents, mixing reactants, incubation, washing and separation (Bound-Free, referred to as B / F), adding signal reagents, measurement, etc. The incubation steps in the above process are generally divided into two types of existing specific implementation technical solutions: fixed time incubation and variable time incubation. Under the fixed time incubation mode, all test incubation times for each test mode are the same. For example, all one-step tests can only achieve 20 minutes of incubation, and all two-step tests can only achieve 10 minutes of incubation for the first time and 10 minutes for the second time. Due to the differences in reagent raw materials, formulas, production processes, reaction principles and conditions for specific test items, this fixed time incubation will increase the difficulty of reagent development or sacrifice certain test performance, such as sensitivity, in actual development and testing, and it is difficult to adapt to multiple different test items. Compared with the constraints and restrictions of the fixed-time incubation method on reagent development and performance, the variable-time incubation method is flexible and highly applicable. The incubation time can be flexibly set according to the different test items, that is, each test item can achieve its own most suitable incubation time. This method can reduce the constraints on reagent development and give full play to the performance of the reagent. In order to achieve variable-time incubation, the existing technical solutions generally use an independent incubation tray that can only achieve incubation. The incubation tray needs to be rotated and stopped multiple times in a test cycle, and the angle of each rotation is determined according to the incubation time. This technical solution has the disadvantages of complex control, high technical implementation difficulty, and unsuitability for high-speed automated testing.

[0004] In addition, the patent number 201710010509.8 discloses a reaction incubation unit, an immunoanalyzer, and a reaction incubation method, wherein the reaction incubation unit includes: a reaction unit for carrying and incubating a reaction container; a transfer unit for moving the reaction container in and out of the reaction unit; the reaction unit includes a rotating unit, an incubation position is set on the rotating unit, and the incubation position is progressively advanced by a predetermined angle θ with the rotating unit at fixed intervals T; the transfer unit moves the reaction container out of the incubation position according to a variable incubation time t1. Although this invention can achieve flexible and variable incubation time, it has the disadvantages of complex transfer unit, high cost, high control difficulty, and can only be adapted to variable time incubation. Summary of the invention

[0005] The purpose of the present invention is to overcome the common shortcomings and problems of the prior art and to provide a card-cage type multi-channel dry immunofluorescence detector.

[0006] The present invention is achieved through the following technical solutions:

[0007] A card-cage type multi-channel dry immunofluorescence detector comprises a base, a test tube rack, test tubes, a first chassis, a puncture sampling device, a clamping and mixing synchronization device, a reagent cup transport device, and an incubation device. The front part of the base is provided with a test tube rack, on which a plurality of test tubes are evenly placed, the left side of the rear part of the base is provided with a clamping and mixing synchronization device, the right side of the rear part of the base is installed with a first chassis, and the first chassis is provided with a puncture sampling device, a reagent cup transport device, a sample extraction and dripping device, and an incubation device.

[0008] Furthermore, the clamping mixing synchronization device includes a second chassis, a first fixed block, a second fixed block, a third fixed block, a fourth fixed block, a first driving wheel, a first passive wheel, a left guide column, a right guide column, a second driving wheel, a second passive wheel, a first motor, a second motor, a fourth motor, a first screw rod, a first belt, a second belt, a first rotating shaft, a first mounting plate, a first guide block, a second guide block, a left connecting plate, a right connecting plate, a left clamping plate, a right clamping plate, a left mounting seat, a right mounting seat, a first screw rod, a first screw rod nut, a fixed plate, a mounting frame, a third driving wheel, a third passive wheel, a fourth driving wheel, a fourth passive wheel, a third belt, a fourth belt, a second mounting plate, a clamping device, a sampling device, a third guide column, a third guide block, and a first screw rod nut.

[0009] The second chassis is provided with a first fixing block, a second fixing block, a third fixing block and a fourth fixing block. The second chassis is also provided with a first motor. The output shaft of the first motor is connected to the first driving wheel. The first driving wheel is connected to the first passive wheel via a first belt. The first passive wheel is connected to the second driving wheel via a first rotating shaft. The second driving wheel is connected to the second passive wheel via a second belt.

[0010] A left guide column is provided between the second fixed block and the first fixed block, and a first guide block that can slide forward and backward along the left guide column is installed on the left guide column, the first guide block is connected to the left connecting plate, a left clamping plate for clamping the first belt is installed at the lower part of the left connecting plate, and a left mounting seat is installed at the upper end of the left connecting plate; a right guide column is provided between the fourth fixed block and the third fixed block, and a second guide block that can slide forward and backward along the right guide column is installed on the right guide column, the second guide block is connected to the right connecting plate, a right clamping plate for clamping the second belt is installed at the lower part of the right connecting plate, and a right mounting seat is installed at the upper end of the right connecting plate.

[0011] A first mounting plate is installed on one side of the left mounting seat, a second motor is installed on the first mounting plate, an output shaft of the second motor is connected to a third driving wheel, the third driving wheel is connected to a third passive wheel through a third belt, the third passive wheel is connected to one end of the first screw rod, the other end of the first screw rod passes through the left mounting seat and is installed in the right mounting seat, a first screw rod nut is provided on the first screw rod, a third guide column is also installed between the left mounting seat and the right mounting seat, a third guide block that can slide left and right along the third guide column is installed on the third guide column, the third guide block and the first screw rod nut are both installed at the lower end of the fixed plate, and the fixed plate is fixedly connected to the clamping mixing unit.

[0012] Furthermore, the left baffle plate and the right baffle plate are installed on the left and right sides of the second mounting plate, a fourth motor is installed on the back side of the second mounting plate, an output shaft of the fourth motor passes through the second mounting plate and is connected to the fourth driving wheel, the fourth driving wheel is connected to the fourth passive wheel through a fourth belt, a left guide rail is provided on the left side of the fourth belt, the left guide rail is installed on the front side of the second mounting plate, a sliding left slider is installed on the left guide rail, the left slider is welded to the left connecting seat, a third clamping plate is installed on one side of the left connecting seat, the third clamping plate clamps the left side of the fourth belt; a first bottom plate is welded on the left connecting seat, a stepping motor is installed on the upper surface of the first bottom plate, and the output of the stepping motor The shaft passes through the first base plate and is fixedly connected to the first cam located below the first base plate, a left swing arm and a right swing arm are respectively provided on the left and right sides of the first cam, a vertical downward pin is installed on the bottom surface of the first base plate, one end of the left swing arm and the right swing arm are movably installed on the pin, the other end of the left swing arm is fixedly connected to the left clamping block, and the left clamping block is installed with a left clamping plate; the other end of the right swing arm is fixedly connected to the right clamping block, and the right clamping block is installed with a right clamping plate; a left connecting block and a right connecting block are respectively welded to the sides of the left swing arm and the right swing arm, the left connecting block and the right connecting block are respectively provided with a first circular hole and a second circular hole, and the first circular hole is connected to the second circular hole by a first spring.

[0013] A right guide rail is provided on the right side of the fourth belt, and the right guide rail is installed on the front side of the second mounting plate. A slidably fitted right slider is installed on the right guide rail, and the right slider is fixedly connected to the right connecting seat, and the left guide rail and the right guide rail are parallel to the fourth belt; a fourth clamping plate is installed on the left side of the right connecting seat, and the fourth clamping plate clamps the right side of the fourth belt; the right connecting seat is in the shape of a channel steel, and is respectively composed of an upper horizontal plate, a lower horizontal plate and a first connecting plate, a first through hole is provided on the upper horizontal plate, and a second through hole is provided on the lower horizontal plate, the liquid extraction needle passes upward through the second through hole and is connected to the needle tube located between the upper horizontal plate and the lower horizontal plate, the top of the needle tube passes through the first through hole and is connected to a micro pump, and the micro pump is connected to a liquid storage cylinder.

[0014] Furthermore, the reagent cup transport device includes a first frame, a driving motor, a fifth driving wheel, a fifth driven wheel and a fifth belt, the first frame is provided with a driving motor, and the two ends of the first frame are respectively provided with a fifth driving wheel and a fifth driven wheel, the fifth driving wheel is connected to the output end of the driving motor through a rotating shaft, the fifth driven wheel is arranged at the other end of the first frame through a rotating shaft, the fifth belt is arranged on the fifth driving wheel and the fifth driven wheel and cooperates with the fifth driving wheel and the fifth driven wheel, a shell is provided above the fifth belt, and it is characterized in that a slide rail is provided on the first frame, a first slider and a second slider matched with the slide rail are provided on the slide rail, a sample cup clamping unit is provided on the first slider and the second slider, and the sample cup clamping unit is fixedly connected to the fifth belt.

[0015] The sample cup clamping unit includes a second connecting plate, a third connecting plate, a second fixing plate, a third fixing plate, a first clamping plate, a second clamping plate, a first arc groove, a second arc groove, a first connecting ear, a second connecting ear, a second spring, a square guide hole, a square guide rod, a circular guide hole, a circular guide rod, a baffle, a first bracket, a second bracket, and a collision block; the second fixing plate is vertically welded to the second connecting plate, the rear side of the second connecting plate is fixedly connected to the first sliding block, the bottom of the second connecting plate is fixedly connected to the fifth belt, the second fixing plate is provided with a first clamping plate, the first clamping plate is provided with a first arc groove, the The front end of the second fixed plate is provided with a first connecting ear; the third fixed plate is vertically welded on the third connecting plate, the rear side of the third connecting plate is fixedly connected to the second slider, the bottom of the third connecting plate is fixedly connected to the fifth belt, a second clamping plate is provided on one side of the third fixed plate, a second arc groove is provided on the surface of the second clamping plate opposite to the first clamping plate, the second arc groove on the second clamping plate cooperates with the first arc groove on the first clamping plate to clamp the sample cup, a second connecting ear is provided at the front end of the third fixed plate, the second connecting ear is connected to the right end of the second spring, and the left end of the second spring is connected to the first clamping plate.

[0016] The first bracket is fixedly installed on the left end of the first frame, a baffle plate facing vertically downward is installed on the bracket, a circular guide rod is welded on the right side of the baffle plate, and a circular guide through hole matched with the circular guide rod is provided on the first clamping plate; the first clamping plate is provided with a square guide rod on the side facing the second clamping plate, and the second clamping plate is provided with a square guide through hole matched with the square guide rod, the second bracket is fixedly installed on the right end of the first frame, and a collision block is installed on the second bracket, and the position of the collision block corresponds to the position of the square guide rod on the first clamping plate.

[0017] The first arc groove and the second arc groove are both semicircular grooves, a left rubber ring is pasted on the top of the first arc groove, and a right rubber ring is pasted on the top of the second arc groove, the left rubber ring and the right rubber ring are both semicircular, the positions of the left rubber ring and the right rubber ring correspond to each other, and the left rubber ring and the right rubber ring form a rubber ring when they are closed, and the inner diameter of the rubber ring is smaller than the outer diameter of the sample cup.

[0018] Further, a housing cavity is provided inside the second clamping plate, the housing cavity is communicated with the second arc groove, a vibration shaking unit is provided in the housing cavity, the vibration shaking unit comprises a first shaking motor, an eccentric wheel, a V-shaped rod, a third bearing seat, a first rotating shaft, and a third spring, the third bearing seat and the first shaking motor are both installed at the bottom of the housing cavity, an eccentric wheel is installed on the output shaft of the first shaking motor, the bottom of the V-shaped rod is fixedly connected to the first rotating shaft, the first rotating shaft is installed in the bearing of the bearing seat, the V-shaped rod consists of a push rod and a rocker, the lower ends of the push rod and the rocker are welded and connected, the push rod and the rocker are V-shaped, and the angle between the push rod and the rocker is 90° to 135°, The push rod is located above the eccentric wheel, and an obliquely installed third spring is provided on the left side of the eccentric wheel. The upper end of the third spring is connected to the push rod, and the lower end of the third spring is fixedly installed on the bottom of the accommodating cavity. The upper end of the rocker is close to the second arc groove in the second clamping plate, and a wear-resistant rubber layer is pasted on the upper end of the rocker.

[0019] Furthermore, the puncture sampling device includes a second frame, a sixth motor, a sixth driving wheel, a sixth driven wheel, a belt, a belt clamp, an upper guide rail, a lower guide rail, an upper slider, a lower slider, a mounting seat, a third mounting plate, a seventh motor, a second screw rod, a first guide column, a second guide column, a guide block, a sampling needle, a needle seat, a first guide hole, a second guide hole, a second screw rod nut, and a cleaning unit.

[0020] The second frame is equipped with a sixth motor, the output shaft of the sixth motor is equipped with a sixth driving wheel, the sixth driving wheel is connected to the sixth driven wheel through a belt, the second frame is equipped with an upper guide rail and a lower guide rail, the upper guide rail is equipped with an upper slider, the lower guide rail is equipped with a lower slider, the upper slider and the lower slider can slide back and forth along the upper guide rail and the lower guide rail, the upper slider and the lower slider are both connected to the mounting seat, the mounting seat is equipped with a belt clamp, the belt clamp is located above the belt and clamps the upper belt, and the mounting seat is equipped with a third mounting plate that is perpendicular to the mounting seat.

[0021] A seventh motor is installed on the third mounting plate, and an output shaft of the seventh motor is connected to the second screw rod. A first guide column and a second guide column are installed on the third mounting plate. A first guide hole, a second guide hole and a second screw nut are provided on the guide block. The first guide column and the second guide column respectively pass through the first guide hole and the second guide hole to form a sliding fit connection. A second screw nut is also installed on the guide block. The second screw rod forms a second screw rod transmission fit connection through the second screw nut. A vertically downward sampling needle is also installed at the front end of the guide block.

[0022] Furthermore, a cleaning unit is provided at the lower part of the mounting seat, and the cleaning unit includes a connecting plate, a cleaning block, a center hole, a water inlet, a water outlet, a spiral groove, and a spiral gap; the connecting plate is fixedly installed at the bottom of the mounting seat, and the cleaning block is fixed to the connecting plate by screw connection or welding. A vertical center hole is provided at the center of the cleaning block, and the position of the center hole corresponds to the position of the sampling needle on the guide block. The sampling needle passes through the center hole and extends to the lower part of the cleaning block. A water inlet and a water outlet are provided on the side of the cleaning block. A spiral groove is provided inside the cleaning block, and the spiral groove is located on the outside of the center hole and spirals up around the center hole. The upper end of the spiral groove is connected to the water outlet, and the lower end of the spiral groove is connected to the water inlet. The spiral groove has a spiral gap on the side facing the center hole, and the spiral gap is communicated with the center hole, and the spiral gap spirals up around the center hole.

[0023] Furthermore, the incubation device comprises a stacked card compartment, an intermediate incubation unit, and a continuous incubation unit, wherein the intermediate incubation unit is disposed behind the stacked card compartment, and the continuous incubation unit is disposed behind the intermediate incubation unit;

[0024] The stacked card warehouse includes a box body, an upper card slot, a partition, a step block, a lower card slot, and a reagent card box. The box body is a rectangular parallelepiped with an opening at the front, and is composed of a left side plate, a right side plate, a lower side plate, an upper side plate, and a back plate. The upper side plate of the box body is provided with a plurality of upper card slots, and the upper card slots are composed of a plurality of partitions evenly spaced apart. The lower side plate of the box body is provided with a plurality of lower card slots, and the lower card slots are composed of a plurality of step blocks evenly spaced apart. The upper card slots correspond to the lower card slots one by one and are adapted to each other. The interval between the upper card slot and the lower card slot on the same vertical line forms a card slot cavity, and a reagent card box is movably installed in the card slot cavity.

[0025] The intermediate incubation unit includes a third frame, an eighth motor, a third screw rod, a third screw rod nut, a second base plate, a third slider, a first guide rail, a vertical plate, a transition holder, a fourth frame, a third base plate, a ninth motor, a fourth screw rod, a fourth screw rod nut, a first connecting block, a fourth slider, a second guide rail, a through hole, and a connecting rod; the eighth motor is installed at the right end of the third frame, the output shaft of the eighth motor is connected to the third screw rod, the third screw rod is provided with a matching third screw rod nut, the third screw rod nut is connected to the second base plate, the bottom surface of the second base plate is installed with a third slider, and the third slider is movably installed on the first guide rail; a vertical plate is vertically installed on one side of the surface of the second base plate, and a transition holder is installed on the upper part of the vertical plate.

[0026] A third base plate is provided at the rear of the third frame, and the third base plate is fixedly connected to the second base plate. A ninth motor is installed on the third base plate, and an output shaft of the ninth motor is connected to a fourth screw rod. A matching fourth screw rod nut is provided on the fourth screw rod, and the fourth screw rod nut is connected to the first connecting block. A fourth slider is installed on the bottom surface of the first connecting block, and the fourth slider is movably installed on the second guide rail and can slide back and forth along the second guide rail. The first connecting block is fixedly connected to a connecting rod, and the connecting rod is fixedly connected to a pull rod.

[0027] The continuous incubation unit includes a fourth frame, a tenth motor, a seventh driving wheel, a seventh belt, a seventh driven wheel, a clamp, and a second slot. The tenth motor is installed at the left end of the fourth frame, the output shaft of the tenth motor is connected to the seventh driving wheel, the seventh driving wheel is connected to the seventh driven wheel through the seventh belt, a plurality of evenly distributed clamps are installed on the seventh belt, a second slot is provided in the clamp, and the size of the second slot is adapted to the reagent card.

[0028] Furthermore, the transition card seat includes a left card plate, a right card plate, a second connecting block, a guide groove, a pull rod, a hook head, an oblique inlet, and an oblique outlet. The left card plate and the right card plate are arranged in parallel and symmetrically. A long strip of gap is provided between the left card plate and the right card plate. A second connecting block is provided below the gap. The second connecting block is fixedly connected to the bottom of the left card plate and the right card plate respectively. An upwardly open guide groove is provided on the second connecting block. A pull rod that can slide back and forth is provided in the guide groove. A hook head is provided at the front end of the pull rod; an opening facing the direction of the gap is provided on the inner side of the left card plate A left card slot is provided on the inner side of the right card plate, and a right card slot with an opening facing the gap direction is provided. The left card slot and the right card slot have the same width and height, and the openings are opposite to each other. The left card slot and the right card slot are symmetrically distributed along the guide groove. The heights of the left card slot and the right card slot are adapted to the thickness of the reagent card, and the distance between the left side surface of the left card slot and the right side surface of the right card slot is adapted to the width of the reagent card. The front ends of the left card slot and the right card slot are both provided with oblique inlets for introducing the reagent card, and the rear ends of the left card slot and the right card slot are both provided with inclined oblique outlets for withdrawing the reagent card.

[0029] Furthermore, the incubation device also includes a scanning unit, which includes a fourth base plate, an eleventh motor, an eighth driving wheel, an eighth belt, an eighth passive wheel, a belt clamp, and a fluorescent signal collector. The fourth base plate is installed on the top of a fourth frame, and the eleventh motor is installed on the fourth base plate. The output shaft of the eleventh motor is connected to the eighth driving wheel, and the eighth driving wheel is connected to the eighth passive wheel through an eighth belt. A belt clamp is installed on the eighth belt, and the belt clamp is fixedly connected to the fluorescent signal collector.

[0030] The present invention has the following technical effects:

[0031] The present invention is provided with a synchronous clamping and mixing device. When the fourth motor drives the fourth driving wheel to rotate, the fourth driven wheel is driven to rotate through the fourth belt. The left side of the fourth belt is connected to the left connecting seat. The left connecting seat is equipped with a stepper motor, a cam, a left swing arm, a right swing arm, a pin shaft, a left clamp, a left clamping plate, and a right clamping block. When the stepper motor drives the cam to rotate 90°, the long axis of the cam rotates to a position approximately perpendicular to the left swing arm and the right swing arm, so that the left swing arm and the right swing arm are opened along the pin shaft, thereby increasing the spacing between the left clamping plate and the right clamping plate, and at the same time stretching the spring to a taut state, so as to achieve the purpose of releasing the reagent cup by the left clamping plate and the right clamping plate. When the stepper motor drives the cam to continue to rotate 90°, the long axis of the cam rotates to a position approximately parallel to the left swing arm and the right swing arm. Under the action of the spring, the spacing between the left swing arm and the right swing arm will be restored to the initial state, thereby clamping the reagent cup. The stepper motor rotates cyclically to achieve the purpose of automatically clamping and releasing the reagent cup; the right side of the fourth belt is connected to the right connecting seat, and the right connecting seat is equipped with a first through hole, a second through hole, a liquid extraction needle, a micro pump, and a liquid storage cylinder. The reagent is extracted into the liquid storage cylinder through the micro pump and the liquid extraction needle. When the left side of the fourth belt rises after clamping the reagent cup, the right side of the fourth belt drops at the same time, and the liquid extraction needle is inserted into the reagent bottle below to extract the reagent; when the right side of the fourth belt rises, the left side of the fourth belt drops to release the reagent cup and clamp another reagent cup, and so on. The cycle is repeated, and multiple functions such as automatic clamping, automatic release, and automatic liquid extraction are realized by reciprocating motion of one belt. The two sides of the belt coordinate and complement each other, which not only greatly improves the detection efficiency and shortens the detection time, but also saves the detection cost and energy consumption.

[0032] The sample cup transport device of the present invention automatically clamps and transports the reagent cup added with the reagent in the synchronous clamping and mixing device. The sample cup transport device of the present invention adopts a novel sample cup clamping unit and a vibration shaking unit with ingenious design and unique conception. The two are integrated into one and complement each other. The vibration shaking is automatically completed in the process of automatic clamping, automatic transportation and automatic release, which not only reduces the production process, shortens the detection time and improves the detection efficiency, but also realizes the seamless connection of various actions, facilitates high-speed automatic detection, and is conducive to the integration and miniaturization of detection equipment. At the same time, it also reduces the workload of detection personnel and reduces the detection cost.

[0033] When the sample cup transport device transports the reagent cup containing the reagent to the bottom of the sampling puncture sampling device, the sampling needle on the puncture sampling device pierces the rubber cap of the test tube placed on the test tube rack to sample the blood or other liquid samples in the test tube, and injects the collected sample into the reagent cup to mix with the reagent for fluorescent marking. The puncture sampling device of the present invention is provided with a cleaning block of a special structure, the center of the cleaning block is provided with a vertical center hole, the interior of the cleaning block is provided with a spiral groove, the spiral groove is located outside the center hole and spirals around the center hole, the upper end of the spiral groove is connected to the water outlet, the lower end of the spiral groove is connected to the water inlet, the spiral groove is provided with a spiral gap on the side facing the center hole, the spiral gap is communicated with the center hole, and the spiral gap spirals around the center hole. During the sampling process, clean water is introduced into the water inlet of the cleaning unit, and the clean water will flow upward along the spiral groove to the water outlet. Under the dual effects of pumping power and spiral centripetal force, the clean water will not overflow from the spiral groove. In the process of flowing through the spiral groove, the residual sample on the sampling needle will be cleaned away through the spiral gap to avoid secondary contamination. This new structure of the puncture sampling device solves the problem of online cleaning of the sampling needle, avoids the trouble of moving the sampling needle to a separately set cleaning pool for cleaning in the existing equipment, reduces the time of additional cleaning process, saves motor energy consumption, reduces costs, and also increases the detection speed, speeds up the detection process, and improves work efficiency.

[0034] In addition, the incubation device of the present invention is different from the existing disc rotating and card inserting incubation units. The incubation device of the present invention mainly includes a stacked card magazine, an intermediate incubation unit, and a continuous incubation unit. The stacked card magazine is composed of multiple groups of card slots, each group of card slots is equipped with a reagent card clip, and each reagent card clip has multiple stacked reagent cards, which are continuously pulled out from the bottom by a pull rod under the action of gravity or elastic force. It has the advantages of large card loading capacity, rapid card output, automatic falling of reagent cards, and suitability for high-speed automated detection. The incubation time interval of the continuous incubation unit of the present invention is fixed, and is suitable for a fixed-time incubation mode; the intermediate incubation unit of the present invention has a novel structure and unique functions, and the reagent card is taken out from the stacked card compartment and sent into the transition card holder. The incubation time in the filter card holder can be controlled by the residence time of the reagent card in the transition card holder. If intermediate incubation is not required, the reagent card can be directly sent into the card clamp of the continuous incubation unit; if intermediate incubation is required, assuming that the intermediate incubation time is △t1, the reagent card can be first incubated for the residence time t1 in the transition card holder, and then sent to the card clamp of the continuous incubation unit for continued fixed-time incubation. Assuming that the fixed incubation time is t2, the total incubation time of the reagent card is △t1 + t2, wherein △t1 is a variable, which can be determined according to different samples and reagents. Therefore, the present invention is suitable for both fixed-time incubation and variable-time incubation, and can be generally used for one-step method, delayed one-step method, and two-step method. It has a wide range of applicability and good flexibility, can save customers a lot of equipment investment, and can also reduce the operating troubles of inspectors.

[0035] In summary, the present invention has the advantages of large card loading capacity, rapid card output, fast detection speed, suitability for high-speed automated detection, etc., and is low in cost and easy to operate. It not only solves the problem that existing detectors cannot simultaneously adapt to multiple incubation methods such as one-step method, delayed one-step method, two-step method, etc., but also solves the problem that the sampling needles of existing detectors cannot be cleaned online. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is the front view of the present invention.

[0037] Figure 2 It is a top view of the present invention.

[0038] Figure 3 It is a three-dimensional stereogram of the present invention.

[0039] Figure 4 This is the front view of the clamping mixing synchronization device.

[0040] Figure 5 A top view of the clamping mixing synchronization device.

[0041] Figure 6 It is a three-dimensional diagram of the clamping mixing synchronization device.

[0042] Figure 7 It is a schematic diagram of the structure of the clamping and mixing unit in the clamping and mixing synchronization device.

[0043] Figure 8 for Figure 7 A partial enlarged view of point A in the figure.

[0044] Fig. 9 for Figure 8 Bottom view of .

[0045] Fig.10 It is a schematic diagram of the structure of the reagent cup transport device.

[0046] Fig.11 It is a schematic diagram of the structure of the sample cup clamping unit in the reagent cup transport device.

[0047] Fig.12 It is a schematic diagram of the structure of the vibration and mixing unit in the reagent cup transport device.

[0048] Fig.13 This is a schematic diagram of the structure of the puncture sampling device.

[0049] Fig.14 It is a cross-sectional view of the cleaning unit in the puncture sampling device.

[0050] Fig.15 Schematic diagram of the structure of the incubation device.

[0051] Fig.16 It is a schematic diagram of the structure of the stacked card compartment in the incubation device.

[0052] Fig.17 It is a schematic diagram of the structure of the intermediate incubation unit in the incubation device.

[0053] Fig.18 It is a schematic diagram of the structure of the transition seat in the incubation device.

[0054] Fig.19 It is a schematic diagram of the structure of the continuous incubation unit in the incubation device.

[0055] Fig. 20 It is a schematic diagram of the structure of the scanning unit in the incubation device.

[0056] Fig.21 It is a schematic diagram of the structure of the card-pickup unit in the incubation device.

[0057] In the accompanying drawings: 1. base, 2. test tube rack, 3. test tube, 4. first chassis, 5. clamping and mixing synchronization device, 6. reagent cup transport device, 7. puncture sampling device, 8. incubation device;

[0058] 501. Second chassis, 502. First fixing block, 503. Second fixing block, 504. Third fixing block, 505. Fourth fixing block, 506. First driving wheel, 507. First passive wheel, 508. Left guide column, 509. Right guide column, 510. Left connecting plate, 511. Right connecting plate, 512. Left clamping plate, 513. Right clamping plate, 514. Left mounting seat, 515. Right mounting seat, 516. First screw rod, 517. First bearing seat, 518. Second bearing seat, 519. First belt, 520. Second belt, 521. First rotating shaft, 522. First mounting plate, 523. First guide block, 524. Second guide block, 525. Second driving wheel, 526. First fixing plate, 527. Second motor, 528. Third driving wheel, 529. Third driven wheel, 530. Third guide column, 531. Third guide block, 532. Third belt, 533. First screw nut, 534. First motor, 535. Second driven wheel, 536. Clamping and mixing unit, 537. Left baffle, 538. Second mounting plate, 539. Right baffle, 540. Fourth motor, 541. Fourth driving wheel, 542. Fourth belt, 543. Fourth passive wheel, 544. Left guide rail, 545. Right guide rail, 546. Left slider, 547. Left connecting seat, 548. First bottom plate, 549. Stepper motor, 550. First spring, 551. Left swing arm, 552. Right swing arm, 553. Left clamping block, 554. Right clamping block, 555. Left clamping plate, 556. Right clamping plate, 557. Left connecting block, 558. First circular hole, 559. Right connecting block, 560. Second circular hole, 561. Pin Axis, 562. light shielding plate, 563. support plate, 564. sensor, 565. third clamping plate, 566. first cam, 567. right slider, 568. upper horizontal plate, 569. first connecting plate, 570. lower horizontal plate, 571. fourth clamping plate, 572. liquid storage cylinder, 573. micro pump, 574. first through hole, 575. large rubber sealing ring, 576. second through hole, 577. small rubber sealing ring, 578. liquid extraction needle, 579. reagent cup;

[0059] 601—first frame, 602—driving motor, 603—fifth driving wheel, 604—fifth belt, 605—fifth driven wheel, 606—sample cup clamping unit, 607—limit sensor, 608—second connecting plate, 609—second fixing plate, 610—blocking plate, 611—circular guide rod, 612—first connecting ear, 613—circular guide through hole, 614—first clamping plate, 615—first circular arc groove, 616—first bracket, 617—square guide rod, 618—square guide through hole, 619—third connecting plate, 620—second circular arc groove, 621—second clamping plate , 622—third fixing plate, 623—second connecting ear, 624—second spring, 625—second bracket, 626—collision block, 627—accommodation chamber, 628—left rubber ring, 629—right rubber ring, 630—rocker, 631—push rod, 632—eccentric wheel, 633—first shaking motor, 634—third spring, 635—first rotating shaft, 636—third bearing seat, 637—first slider, 638—second slider, 639—slide rail, 640—sample cup, 641—housing, 642—mounting hole, 643—transverse plate, 644—vertical plate, 645—support plate;

[0060] 701. second frame, 702. sixth motor, 703. sixth driving wheel, 704. sixth driven wheel, 705. sixth belt, 706. belt clamp, 707. upper guide rail, 708. lower guide rail, 709. upper slider, 710. lower slider, 711. mounting seat, 712. third mounting plate, 713. seventh motor, 714. second screw rod, 715. first guide column, 716. second guide column, 717. guide block, 718. liquid inlet, 719. threaded hole, 720. sampling needle, 721. needle seat, 722. first guide hole, 723. second guide hole, 724. second screw rod nut, 725. cleaning unit, 726. water inlet, 727. spiral groove, 728. water outlet, 729. connecting plate, 730. cleaning block, 731. spiral gap, 732. center hole;

[0061] 81. stacked card compartment, 8101. box body, 8102. exhaust port, 8103. grid hole, 8104. exhaust fan, 8105. cooling fan, 8106. card slot cavity, 8107. first step, 8108. partition, 8109. second step, 8110. upper card slot, 8111. step block, 8112. lower card slot, 8113. reagent card box, 8114. handle, 8115. left side plate, 8116. right side plate, 8117. lower side plate, 8118. upper side plate, 8119. back plate; 82. intermediate incubation unit, 8201. third rack, 8202. eighth motor, 8203. third screw rod, 8204. third screw rod nut, 8205. second bottom plate, 8206. vertical plate, 8207. transition card seat, 8208. first slider, 8209. first guide rail, 8210. third bottom plate, 8211. ninth motor, 8212. fourth screw rod, 8213. fourth screw rod nut, 8214. first connecting block, 8215. second slider, 8216. second guide rail, 8217. connecting rod, 8218. pull rod, 8219. left card plate, 8220. right card plate, 8221. left card slot, 8222. right card slot, 8223. second connecting block, 8224. guide slot, 8225. hook head, 8226. oblique inlet, 8227. oblique outlet; 83. Continuous incubation unit, 8301. Fourth frame, 8302. Tenth motor, 8303. Seventh driving wheel, 8304. Seventh belt, 8305. Seventh passive wheel, 8306. Card clamp, 8307. Second card slot; 84. Scanning unit, 8401. Fourth bottom plate, 8402. Eleventh motor, 8403. Eighth driving wheel, 8404. Eighth belt, 8405. Eighth passive wheel, 8406. Fluorescence signal collector, 8407. Belt clamp; 85. Card picking unit, 8501. Twelfth motor, 8502. Fifth screw rod, 8503. Fifth screw rod nut, 8504. Third connecting block, 8505. Third slider, 8506. Third guide rail, 8507. Pickup rod. DETAILED DESCRIPTION

[0062] The present invention will be further described in detail below through specific implementation modes in conjunction with the accompanying drawings.

[0063] like Figures 1 to 3As shown, a card-cage type multi-channel dry immunofluorescence detector includes a base 1, a test tube rack 2, a test tube 3, a first chassis 4, a clamping and mixing synchronization device 5, a puncture sampling device 7, a reagent cup transport device 6, and an incubation device 8. A test tube rack 2 is rotatably arranged at the front of the base 1, and a plurality of test tubes 3 are evenly placed on the test tube rack 2, and blood or other liquid samples are stored in the test tubes 3. A clamping and mixing synchronization device 5 is arranged on the left side of the rear part of the base 1, and the clamping and mixing synchronization device 5 is used to clamp the reagent cup and inject the reagent into the reagent cup. A first chassis 4 is installed on the right side of the rear part of the base 1, and a puncture sampling device 7, a reagent cup transport device 6 and an incubation device 8 are arranged in the first chassis 4. The reagent cup transport device 6 is used to transport the reagent cup that has been injected with the reagent, and first transports the reagent cup to the bottom of the puncture sampling device 7. The puncture sampling device 7 is used to pierce the rubber cap at the top of the test tube 3 and extract the sample in the test tube 3, and inject the sample into the reagent cup filled with the reagent. Then the reagent cup transport device 6 transports the reagent cup to the incubation device 8, and then the sample marked with the reagent in the reagent cup is dripped into the reagent card of the incubation device 8 through an automatic sampling device or manual dripping. The incubation device 8 is used for sample incubation and scanning detection.

[0064] like Figures 4-5 As shown, the clamping mixing synchronization device 5 includes a second chassis 501, a first fixing block 502, a second fixing block 503, a third fixing block 504, a fourth fixing block 505, a first driving wheel 506, a first driven wheel 507, a left guide column 508, a right guide column 509, a left connecting plate 510, a right connecting plate 511, a left clamping plate 512, a right clamping plate 513, a left mounting seat 514, a right mounting seat 515, a first screw rod 516, a first bearing seat 517, a second Bearing seat 518, first belt 519, second belt 520, first rotating shaft 521, inlay, first guide block 523, second guide block 524, second driving wheel 525, first fixing plate 526, second motor 527, third driving wheel 528, third driven wheel 529, third guide column 530, third guide block 531, third belt 532, first screw nut 533, first motor 534, second driven wheel 535, clamping and mixing unit 536.

[0065] The first fixing block 502, the second fixing block 503, the third fixing block 504 and the fourth fixing block 505 are fixedly mounted on the second chassis 501 by screws, and the first motor 534 is mounted on the second chassis 501 by bolts, and the output shaft of the first motor 534 is connected to the first driving wheel 506. The first driving wheel 506 is connected to the first passive wheel 507 by the first belt 519, and the first passive wheel 507 is connected to the second driving wheel 525 by the first rotating shaft 521. A first bearing seat 517 is mounted near the first passive wheel 507, and a second bearing seat 518 is mounted near the second driving wheel 525. The left end of the first rotating shaft 521 passes through the first bearing seat 517 to connect with the first passive wheel 507, and the right end of the first rotating shaft 521 passes through the second bearing seat 518 to connect with the second driving wheel 525, and the second driving wheel 525 is connected to the second passive wheel 535 by the second belt 520. When the first motor 534 is started, it drives the first driving wheel 506 to rotate, thereby driving the first belt 519 to rotate, the first belt 519 drives the first driven wheel 507 to rotate, and then the first driven wheel 507 drives the first rotating shaft 521 to rotate, and then drives the second driving wheel 525 to rotate synchronously, so that the second belt 520 drives the second driven wheel 535 to rotate.

[0066] The second fixing block 503 is connected to the first fixing block 502 via a left guide column 508, on which a first guide block 523 capable of sliding forward and backward along the left guide column 508 is installed, and the first guide block 523 is welded to the left connecting plate 510. A left clamping plate 512 for clamping the first belt 519 is installed at the lower part of the left connecting plate 510 via bolts, and a left mounting seat 514 is welded to the upper end of the left connecting plate 510. The fourth fixing block 505 is connected to the third fixing block 504 via a right guide column 509, on which a second guide block 524 capable of sliding forward and backward along the right guide column 509 is installed, and the second guide block 524 is welded to the right connecting plate 511. A right clamping plate 513 for clamping the second belt 520 is installed at the lower part of the right connecting plate 511 via bolts, and a right mounting seat 515 is welded to the upper end of the right connecting plate 511.

[0067] A first mounting plate 522 is mounted on one side of the left mounting seat 514, a second motor 527 is mounted on the first mounting plate 522, and an output shaft of the second motor 527 is connected to a third driving wheel 528. The third driving wheel 528 is connected to a third driven wheel 529 via a third belt 532, and the third driven wheel 529 is welded to one end of the first screw rod 516. Bearings are provided in both the left mounting seat 514 and the right mounting seat 515, and both ends of the first screw rod 516 are respectively mounted in the left mounting seat 514 and the right mounting seat 515 via bearings, and a first screw rod nut 533 is provided on the first screw rod 516. A third guide block 30 is also installed between the left mounting seat 514 and the right mounting seat 515. A third guide block 531 capable of sliding left and right along the third guide block 30 is installed on the third guide block 30. The third guide block 531 and the first screw nut 533 are both welded and installed on the lower end of the first fixed plate 526. The first fixed plate 526 is fixedly installed on the clamping and mixing unit 536. When the second motor 527 is started, the third driving wheel 528 is driven to rotate, thereby driving the second driven wheel 535 to rotate, so that the first screw nut 533 on the first screw 516 can move back and forth along the first screw 516. Then the clamping and mixing unit 536 is driven to move left and right along the third guide block 30, so that reagent cups in different directions can be clamped and sampled.

[0068] like Figure 7 , Figure 8 , Fig. 9 As shown, the clamping and mixing unit 536 includes a left baffle 537, a right baffle 539, a second mounting plate 538, a fourth motor 540, a fourth driving wheel 541, a fourth belt 542, a fourth driven wheel 543, a left guide rail 544, a left slider 546, a left connecting seat 547, a right guide rail 545, a right slider 567, a right connecting seat 579, a third clamping plate 565, a fourth clamping plate 571, a first bottom plate 548, a stepping motor 549, a first cam 566, a left swing arm 551, a right swing arm 552, and a pin 56 1. Left clamping block 553, right clamping block 555, right clamping block 554, right clamping plate 556, left connecting block 557, right connecting block 559, first circular hole 558, second circular hole 560, first spring 550, light shielding plate 562, support plate 563, sensor 564, upper horizontal plate 568, lower horizontal plate 570, first connecting plate 569, first through hole 574, large rubber sealing ring 575, small rubber sealing ring 577, second through hole 576, liquid extraction needle 578, micro pump 573, liquid storage cylinder 572.

[0069] The left baffle plate 537 and the right baffle plate 539 are fixedly mounted on the left and right sides of the second mounting plate 538 by welding or bolting. The fourth motor 540 is bolted to the upper back of the second mounting plate 538, and the first fixing plate is vertically welded to the lower back of the second mounting plate 538. The output shaft of the fourth motor 540 passes through the second mounting plate 538 and is connected to the fourth driving wheel 541, and the fourth driving wheel 541 is connected to the fourth driven wheel 543 by the fourth belt 542. A left guide rail 544 is provided on the left side of the fourth belt 542. The left guide rail 544 is mounted on the front of the second mounting plate 538 by countersunk screws. A left slider 546 is mounted on the left guide rail 544 in a sliding fit. The left slider 546 is welded to the left connecting seat 547. A third clamping plate 565 is bolted to one side of the left connecting seat 547, and the third clamping plate 565 clamps the left side of the fourth belt 542. A first base plate 548 is welded to the left connecting seat 547, and a stepper motor 549 is mounted on the upper surface of the first base plate 548. The output shaft of the stepper motor 549 passes through the first base plate 548 and is welded to a first cam 566 located below the first base plate 548. A left swing arm 551 and a right swing arm 552 are respectively provided on the left and right sides of the first cam 566. A vertically downward pin 561 is mounted on the bottom surface of the first base plate 548, and one end of the left swing arm 551 and the right swing arm 552 are both movably mounted on the pin 561 and are in rotational cooperation with the pin 561. A left clamping block 553 is welded to the other end of the left swing arm 551, and a right clamping block 555 is welded to the left clamping block 553. A right clamping block 554 is welded to the other end of the right swing arm 552, and a right clamping plate 556 is welded to the right clamping block 554. The left clamping block 553 and the right clamping plate 556 are both arc-shaped, and their shapes are compatible with the seventh belt 579.

[0070] A left connecting block 557 and a right connecting block 559 are welded to the sides of the left swing arm 551 and the right swing arm 552, respectively. A first circular hole 558 and a second circular hole 560 are respectively provided on the left connecting block 557 and the right connecting block 559. The first circular hole 558 and the second circular hole 560 are connected by a first spring 550. A light shielding plate 562 is also installed on the side of the right swing arm 552, and a support plate 563 is welded to the side of the first bottom plate 548, and a sensor 564 is installed on the support plate 563 by screws. When the stepper motor 549 drives the first cam 566 to rotate 90°, the long axis of the first cam 566 rotates to a position approximately perpendicular to the left swing arm 551 and the right swing arm 552, so that the left swing arm 551 and the right swing arm 552 are opened along the pin 561, thereby increasing the distance between the right clamping block 555 and the right clamping plate 556, and at the same time stretching the first spring 550 to a tight state, so as to achieve the purpose of releasing the seventh belt 579 by the right clamping block 555 and the right clamping plate 556; when the stepper motor 549 drives the first cam 566 to continue to rotate 90°, the long axis of the first cam 566 rotates to a position approximately parallel to the left swing arm 551 and the right swing arm 552, and under the action of the first spring 550, the distance between the left swing arm 551 and the right swing arm 552 will be restored to the initial state, thereby clamping the seventh belt 579. The stepper motor 549 rotates cyclically, so as to achieve the purpose of automatically clamping and releasing the seventh belt 579.

[0071] A right guide rail 545 is provided on the right side of the fourth belt 542. The right guide rail 545 is installed on the front side of the second mounting plate 538 by countersunk screws. A right slider 567 is installed on the right guide rail 545 for sliding fit. The right slider 567 is welded and connected to a right connecting seat 579. The left guide rail 544 and the right guide rail 545 are parallel to the fourth belt 542. A fourth clamping plate 571 is installed on the left side of the right connecting seat 579 by bolts. The fourth clamping plate 571 clamps the right side of the fourth belt 542. The right connecting seat 579 is in the shape of a channel steel, and is respectively composed of an upper transverse plate 568, a lower transverse plate 570 and a first connecting plate 569. The upper transverse plate 568 is provided with a first through hole 574, and the lower transverse plate 570 is provided with a second through hole 576. The liquid extraction needle 578 passes upward through the second through hole 576 and is connected to the needle tube between the upper horizontal plate 568 and the lower horizontal plate 570. The top of the needle tube passes through the first through hole 574 and is connected to the micro pump 573. The micro pump 573 is connected to the upper liquid storage cylinder 572. The first through hole 574 is located directly above the second through hole 576, and the diameter of the first through hole 574 is larger than the diameter of the second through hole 576. A large rubber sealing ring 575 for sealing and fixing the needle tube is installed in the first through hole 574, and a small rubber sealing ring 577 for sealing and fixing the liquid extraction needle 578 is installed in the second through hole 576.

[0072] like Fig.10 As shown, the reagent cup transport device 6 includes a first frame 601, a driving motor 602, a fifth driving wheel 603, a fifth driven wheel 605 and a fifth belt 604. The first frame 601 is provided with a driving motor 602, and the first frame 601 is provided with a fifth driving wheel 603 and a fifth driven wheel 605 at both ends. The fifth driving wheel 603 is connected to the output end of the driving motor 602 through a rotating shaft, and the fifth driven wheel 605 is arranged at the other end of the first frame 601 through a rotating shaft. The fifth belt 604 is arranged on the fifth driving wheel 603 and the fifth driven wheel 605 and cooperates with the fifth driving wheel 603 and the fifth driven wheel 605. A housing 641 is provided above the fifth belt 604. The housing 641 is L-shaped and includes a horizontal plate 643 and a vertical plate 644. The lower end of the vertical plate 644 is welded to the first frame 601. The upper end of the vertical plate 644 is connected to the horizontal plate 643 and is perpendicular to the horizontal plate 643. The horizontal plate 643 is perpendicular to the first frame 601. The horizontal plate 643 is provided with a mounting hole 642. It is characterized in that a slide rail 639 is installed on the first frame 601 by countersunk screws. The slide rail 639 is parallel to the fifth belt 604. The slide rail 639 is provided with a first slider 637 and a second slider 638 adapted to the slide rail 639. The sample cup clamping unit 606 is installed on the first slider 637 and the second slider 638. A support plate 645 for supporting the sample cup 640 is also installed at the lower part of the first frame 601 . The support plate 645 is parallel to the fifth belt 604 . The support plate 645 is located directly below the sample cup clamping unit 606 .

[0073] like Fig.10 , Fig.11 As shown, the sample cup clamping unit 606 includes a second connecting plate 608, a third connecting plate 619, a second fixing plate 609, a third fixing plate 622, a first clamping plate 614, a second clamping plate 621, a first connecting ear 612, a second connecting ear 623, a second spring 624, a square guide hole 618, a square guide rod 617, a circular guide hole 607, a circular guide rod 611, a baffle 610, a first bracket 616, a second bracket 625, a collision block 626, a first arc groove 615, a second arc groove 620, a left rubber ring 628, a right rubber ring 629, and an accommodating cavity 627.

[0074] The second fixing plate 609 is vertically welded to the second connecting plate 608. The rear side of the second connecting plate 608 is connected to the first slider 637 by countersunk screws or welding. The bottom of the second connecting plate 608 is fixedly connected to the upper side of the fifth belt 604 by bolts. The second fixing plate 609 is provided with a first clamping plate 614. The second connecting plate 608, the second fixing plate 609 and the first clamping plate 614 are all made of stainless steel plates. The first clamping plate 614 is vertically welded to the upper surface of the second fixing plate 609. The right side of the first clamping plate 614 is provided with a first arc groove 615. The front end of the second fixing plate 609 is provided with a first connecting ear 612 by welding or cutting.

[0075] The third fixing plate 622 is vertically welded to the third connecting plate 619, and the rear side of the third connecting plate 619 is fixedly connected to the second slider 638 by countersunk screws or welding. A second clamping plate 621 is welded to one side of the third fixing plate 622, and the third connecting plate 619, the third fixing plate 622, and the second clamping plate 621 are all made of stainless steel plates. A second arc groove 620 is opened on the side of the second clamping plate 621 opposite to the first clamping plate 614, and the second arc groove 620 on the second clamping plate 621 cooperates with the first arc groove 615 on the first clamping plate 614 to clamp the sample cup 640. The front end of the third fixing plate 622 is provided with a second connecting ear 623 by welding or cutting, and the second connecting ear 623 is connected to the right end of the second spring 624, and the left end of the second spring 624 is connected to the first connecting ear 612.

[0076] The first bracket 616 is welded and installed at the left end of the first frame 601. A baffle plate 610 is installed vertically downward on the bracket by screw connection or welding. A circular guide rod 611 is welded on the right side of the baffle plate 610. A circular guide through hole 607 matching the circular guide rod 611 is provided on the first clamping plate 614. The circular guide rod 611 passes through the circular guide through hole 607 and can reach the second clamping plate 621. The first clamping plate 614 is provided with a square guide rod 617 on the side facing the second clamping plate 621 by welding or inlay fixing. The second clamping plate 621 is provided with a square guide through hole 618 matching the square guide rod 617. The square guide rod 617 on the right side of the first clamping plate 614 passes through the square guide through hole 618 on the second clamping plate 621 and extends to the outside of the second clamping plate 621. The square guide rod 617 plays a role of guidance and collision. The second bracket 625 is welded and installed at the right end of the first frame 601. A collision block 626 is installed on the bottom surface of the second bracket 625 by screw connection or welding. The position of the collision block 626 corresponds to the position of the square guide rod 617 on the first clamping plate 614. In addition, a limit sensor 607 is installed on both the first bracket 616 and the second bracket 625.

[0077] The first arc groove 615 and the second arc groove 620 are both semicircular grooves. A left rubber ring 628 is pasted on the upper part of the first arc groove 615, and a right rubber ring 629 is pasted on the upper part of the second arc groove 620. The left rubber ring 628 and the right rubber ring 629 are both semicircular. The positions of the left rubber ring 628 and the right rubber ring 629 correspond to each other. When the left rubber ring 628 and the right rubber ring 629 are closed, they form a rubber ring. The inner diameter of the rubber ring is smaller than the outer diameter of the sample cup 640. The left rubber ring 628 and the right rubber ring 629 are used to play a buffering elastic role when clamping the sample cup 640, and also play an auxiliary shaking role when the vibration shaking unit is in action.

[0078] like Fig.12 As shown, a vertical accommodating cavity 627 is provided inside the second clamping plate 621 , and the accommodating cavity 627 is communicated with the second arc groove 620 . A vibration and shaking unit is provided inside the accommodating cavity 627 .

[0079] The vibration and even-shaking unit includes a first even-shaking motor 633, an eccentric wheel 632, a V-shaped rod, a third bearing seat 636, a first rotating shaft 635, and a third spring 634. The third bearing seat 636 and the first even-shaking motor 633 are fixedly mounted at the bottom of the accommodating chamber 627 by bolts, an eccentric wheel 632 is mounted on the output shaft of the first even-shaking motor 633, the bottom of the V-shaped rod is fixedly connected to the first rotating shaft 635, and the first rotating shaft 635 is mounted in the bearing of the bearing seat. The V-shaped rod consists of a push rod 631 and a rocking arm 630, the lower ends of the push rod 631 and the rocking arm 630 are welded and connected, the push rod 631 and the rocking arm 630 are V-shaped, and the angle between the push rod 631 and the rocking arm 630 is 90° to 135°. The push rod 631 is located above the eccentric wheel 632, and an obliquely installed third spring 634 is provided on the left side of the eccentric wheel 632. The upper end of the third spring 634 is connected to the push rod 631 by welding or circular hole connection, and the lower end of the third spring 634 is fixedly installed at the bottom of the accommodating cavity 627. The upper end of the rocker 630 is close to the second arc groove 620 on the second clamping plate 621, and a wear-resistant rubber layer is pasted on the upper end of the rocker 630.

[0080] When the first shaking motor 633 is started, it drives the eccentric wheel 632 to rotate, pushing the push rod 631 of the V-shaped rod to swing up and down, thereby driving the rocker 630 connected to the push rod 631 to continuously swing around the first rotating shaft 635, thereby achieving vibration and shaking of the sample cup 640 located between the first clamping plate 614 and the second clamping plate 621.

[0081] like Fig.13 , Fig.14 As shown, the puncture sampling device 7 includes a second frame 701, a sixth motor 702, a sixth driving wheel 703, a sixth driven wheel 704, a sixth belt 705, a sixth belt clamp 706, an upper guide rail 707, a lower guide rail 708, an upper slider 709, a lower slider 710, a mounting seat 711, a third mounting plate 712, a seventh motor 713, a second screw rod 714, a first guide column 715, a second guide column 716, a guide block 717, a sampling needle 720, a needle seat 721, a first guide hole 722, a second guide hole 723, a second screw rod nut 724, and a cleaning unit 725.

[0082] The second frame 701 is provided with a sixth motor 702 by bolts, and the output shaft of the sixth motor 702 is provided with a sixth driving wheel 703 by key connection, and the sixth driving wheel 703 is connected to the sixth driven wheel 704 by a sixth belt 705. The upper and lower sides of the second frame 701 are provided with upper guide rails 707 and lower guide rails 708 which are parallel to each other by screws, respectively, and the upper slider 709 is provided on the upper guide rail 707, and the lower slider 710 is provided on the lower guide rail 708. The upper slider 709 and the lower slider 710 can slide back and forth along the upper guide rail 707 and the lower guide rail 708, and the upper slider 709 and the lower slider 710 are welded to the mounting seat 711. The mounting seat 711 is provided with a sixth belt clamp 706 by screws, and the sixth belt clamp 706 is located above the sixth belt 705 and clamps the upper side of the sixth belt 705. The mounting seat 711 is bolted with a third mounting plate 712 which is perpendicular to the mounting seat 711. The sixth motor 702 drives the sixth driving wheel 703 to rotate, thereby driving the sixth belt 705 to rotate. The sixth belt 705 is clamped by the sixth belt clamp 706, thereby driving the mounting seat 711 to slide back and forth along the upper guide rail 707 and the lower guide rail 708.

[0083] The seventh motor 713 is mounted on the third mounting plate 712 by screws, and the output shaft of the seventh motor 713 is connected to the second screw rod 714 by welding or coupling. The first guide column 715 and the second guide column 716 are welded and mounted vertically on the third mounting plate 712, and the guide block 717 is provided with a vertical first guide hole 722 and a second guide hole 723. The first guide column 715 and the second guide column 716 pass through the first guide hole 722 and the second guide hole 723 respectively, and the guide block 717 is in sliding cooperation with the first guide column 715 and the second guide column 716. The front end of the guide block 717 is provided with a threaded hole 719, and the lower end of the sampling needle 720 is installed vertically downward through the threaded hole 719, and the upper end of the sampling needle 720 is provided with a needle seat 721 with external threads, and the needle seat 721 is fixedly installed in the threaded hole 719 by threaded connection. The lower part of the sampling needle 720 is provided with a liquid inlet hole 718, and the lower end of the sampling needle 720 is in a pointed cone shape, which is convenient for inserting through the rubber cap of the test tube and extending into the test tube to collect samples. The guide block 717 is also installed with a second screw nut 724 on the side of the first guide hole 722 and the second guide hole 723, and the second screw 714 forms a second screw transmission matching connection through the second screw nut 724. When the seventh motor 713 drives the second screw 714 to rotate, the guide block 717 can slide up and down along the first guide column 715 and the second guide column 716, thereby driving the sampling needle 720 to sample samples of different depths.

[0084] A cleaning unit 725 is provided at the lower part of the mounting seat 711. As shown in FIG714, the cleaning unit 725 includes a connecting plate 729, a cleaning block 730, a central hole 732, a water inlet 726, a water outlet 728, a spiral groove 727, and a spiral gap 731.

[0085] The connecting plate 729 is welded to the bottom of the mounting seat 711, and the cleaning block 730 is fixed to the connecting plate 729 by screw connection or welding. The cleaning block 730 is rectangular, square, spherical, ellipsoidal or other shapes, and a vertical center hole 732 is opened in the center of the cleaning block 730, and the position of the center hole 732 corresponds to the position of the sampling needle 720 on the guide block 717. The center hole 732 is a cylindrical hole, and the radius of the center hole 732 is 0.1-0.5mm larger than the radius of the sampling needle 720. The sampling needle 720 passes through the center hole 732 and extends to the lower part of the cleaning block 730. The side of the cleaning block 730 is provided with a water inlet 726 and a water outlet 728, and the interior of the cleaning block 730 is provided with a spiral groove 727, which is located on the outside of the center hole 732 and spirals upward around the center hole 732. The spiral groove 727 is provided with a spiral gap 731 on the side facing the center hole 732, and the spiral gap 731 is communicated with the center hole 732 and spirals upward around the center hole 732.

[0086] Preferably, the spiral groove 727 is a circular tubular spiral groove, and since a spiral slit 731 is formed inside the spiral groove 727, the cross section of the spiral groove 727 is C-shaped, and the width of the spiral slit 731 is 1 / 3 to 1 / 2 of the inner diameter of the spiral groove 727. The upper end of the spiral groove 727 is connected to the water outlet 728, and the lower end of the spiral groove 727 is connected to the water inlet 726.

[0087] The water inlet 726 is connected to the clean water tank through a pipeline (not shown in the figure), and the water outlet 728 is connected to the waste water tank through a pipeline and a water pump (not shown in the figure). During the sampling process, the water pump is started, and the clean water will flow upward along the spiral groove 727 to the water outlet 728. Due to the centripetal force, the clean water will not overflow from the spiral gap 731. In the process of flowing through the spiral groove 727, the sample remaining on the sampling needle 720 is washed away to avoid secondary contamination, which not only reduces the trouble of adding an additional cleaning process, saves costs, but also speeds up the detection process and improves work efficiency.

[0088] like Fig.15As shown, the incubation device 88 includes a stacked card bin 81, an intermediate incubation unit 82, a continuous incubation unit 83, a scanning unit 84, and a card-pickup unit 85. The intermediate incubation unit 82 is disposed behind the stacked card bin 81, and the continuous incubation unit 83 is disposed behind the intermediate incubation unit 82. The scanning unit 84 and the card-pickup unit 85 are installed above the continuous incubation unit 83. A large number of stacked reagent cards are contained in the stacked card bin 81. The intermediate incubation unit 82 hooks the reagent card out of the stacked card bin 81, and performs intermediate incubation or transfers the reagent card to the continuous incubation unit 83 for continued incubation. The scanning unit 84 performs scanning and detection, and the card-pickup unit 85 pulls out the reagent card.

[0089] like Fig.16 As shown, the stacked card compartment 81 includes a box body 8101, an exhaust port 8102, a grid hole 8103, an exhaust fan 8104, a cooling fan 8105, a partition 8108, an upper card slot 8110, a step block 8111, a lower card slot 8112, a reagent card box 8113, and a handle 8114.

[0090] The box body 8101 is welded by a left side plate 8115, a right side plate 8116, a lower side plate 8117, an upper side plate 8118 and a back plate 8119. An exhaust port 8102 is provided on the upper surface of the upper side plate 8118, and an exhaust fan 8104 is fixedly installed at the exhaust port 8102 by screws. A grid hole 8103 is provided on the back plate 8119, and a cold air fan 8105 is installed at the grid hole 8103 by bolts. By conveying cold air from the grid hole 8103 to the box body 8101 through the cold air fan 8105, the entire box body 8101 is ensured to be in a low temperature state, thereby extending the service life of the reagent card. The gas is conveyed out from the exhaust port 8102 by the exhaust fan 8104, thereby achieving an air convection effect and ensuring the air cleanliness of the entire box body 8101.

[0091] The upper side plate 8118 is provided with a plurality of upper slots 8110 on the inner side thereof, and the upper slots 8110 are formed by a plurality of partitions 8108 of uniform size and evenly spaced, and the partitions 8108 are fixed to the inner wall above the box body 8101 by welding or screws. The lower side plate 8117 at the bottom of the box body 8101 is provided with a plurality of lower slots 8112, and the lower slots 8112 are formed by a plurality of step blocks 8111 of uniform size and evenly spaced, and the step blocks 8111 are fixed to the lower side plate 8117 at the lower end of the box body 8101 by welding or bolts. The step blocks 8111 are composed of a first step 8107 and a second step 8109, and the upper slots 8110 correspond to the lower slots 8112 one by one and are adapted to each other, and the interval between the upper slots 8110 and the lower slots 8112 on the same vertical line forms a slot cavity 8106. The card slot cavity 8106 contains a reagent card box 8113, and the reagent card box 8113 is filled with reagent cards.

[0092] like Fig.17 As shown, the intermediate incubation unit 82 includes a third frame 8201, an eighth motor 8202, a third screw rod 8203, a third screw rod nut 8204, a second base plate 8205, a third slider 8208, a first guide rail 8209, a vertical plate 8206, a transition bracket 8207, a third base plate 8210, a ninth motor 8211, a fourth screw rod 8212, a fourth screw rod nut 8213, a first connecting block 8214, a fourth slider 8215, a second guide rail 8216, and a connecting rod 8217.

[0093] The right end of the third frame 8201 is equipped with an eighth motor 8202, the output shaft of the eighth motor 8202 is connected to the third screw rod 8203, and the third screw rod 8203 is provided with a matching third screw rod nut 8204. The third screw rod nut 8204 is welded to the second bottom plate 8205, and the bottom surface of the second bottom plate 8205 is equipped with two cylindrical third sliders 8208 by welding or bolt connection. The two third sliders 8208 are respectively movably mounted on two mutually parallel round rod-shaped first guide rails 8209, and can slide back and forth along the first guide rails 8209, and both ends of the two first guide rails 8209 are fixed on the third frame 8201.

[0094] A vertical plate 8206 is vertically welded to one side of the surface of the second bottom plate 8205, and a transition seat 8207 is installed on the upper part of the vertical plate 8206 by welding or bolt connection. Fig.18As shown, the transition base 8207 includes a left card plate 8219, a left card slot 8221, a right card plate 8220, a right card slot 8222, a second connecting block 8223, a guide slot 8224, a pull rod 8218, a hook head 8225, an oblique inlet 8226, and an oblique outlet 8227. The left card plate 8219 and the right card plate 8220 are arranged parallel and symmetrically. An elongated gap is provided between the left card plate 8219 and the right card plate 8220. An elongated second connecting block 8223 is provided below the gap. The elongated second connecting block 8223 is welded to the bottom of the left card plate 8219 and the bottom of the right card plate 8220, respectively. The second connecting block 8223 is provided with an upwardly opening guide groove 8224, the guide groove 8224 is a rectangular guide groove 8224, a pull rod 8218 that can slide back and forth is arranged in the guide groove 8224, and a hook head 8225 is installed at the front end of the pull rod 8218. Preferably, the hook head 8225 is trapezoidal, and the hook head 8225 is a telescopic hook head or a rotating hook head.

[0095] A left card slot 8221 with an opening toward the gap direction is provided on the inner side of the left card plate 8219, and a right card slot 8222 with an opening toward the gap direction is provided on the inner side of the right card plate 8220. The left card slot 8221 and the right card slot 8222 have the same width and height, and their openings are opposite to each other. The left card slot 8221 and the right card slot 8222 are symmetrically distributed along the guide groove 8224. The height of the left card slot 8221 and the right card slot 8222 is adapted to the thickness of the reagent card, and the distance between the left side surface of the left card slot 8221 and the right side surface of the right card slot 8222 is adapted to the width of the reagent card. The front ends of the left card slot 8221 and the right card slot 8222 are both provided with an oblique inlet 8226 for introducing the reagent card, and the rear ends of the left card slot 8221 and the right card slot 8222 are both provided with an inclined oblique outlet 8227 for withdrawing the reagent card.

[0096] A third base plate 8210 is provided at the rear of the third frame 8201, and the third base plate 8210 is fixedly connected to the second base plate 8205 by welding. A ninth motor 8211 is fixedly installed on the third base plate 8210 by bolts, and the output shaft of the ninth motor 8211 is connected to the fourth screw rod 8212 by a coupling, and a fourth screw rod nut 8213 is provided on the fourth screw rod 8212. The fourth screw rod nut 8213 is fixedly connected to the first connecting block 8214 by welding or bolt connection, and a fourth slider 8215 is installed on the bottom surface of the first connecting block 8214 by welding or countersunk screw connection. The fourth slider 8215 is movably installed on the second guide rail 8216 and can slide back and forth along the second guide rail 8216. The second guide rail 8216 is parallel to the guide groove 8224 in the transition seat 8207, and the second guide rail 8216 is fixedly installed on the third base plate 8210 by countersunk screws. A connecting rod 8217 is welded to the right side of the first connecting block 8214 , and the connecting rod 8217 is welded to the rear end of the pull rod 8218 .

[0097] like Fig.19 As shown, the continuous incubation unit 83 includes a fourth frame 8301, a tenth motor 8302, a seventh driving wheel 8303, a seventh belt 8304, a seventh driven wheel 8305, a clamp 8306, and a second slot 8307. The tenth motor 8302 is installed at the left end of the fourth frame 8301. The output shaft of the tenth motor 8302 is connected to the seventh driving wheel 8303, and the seventh driving wheel 8303 is connected to the seventh driven wheel 8305 through the seventh belt 8304. A plurality of evenly distributed clamps 8306 are installed on the seventh belt 8304 through countersunk screws. The clamp 8306 is provided with a second slot 8307 for placing a reagent card, and the size of the second slot 8307 is adapted to the reagent card.

[0098] When the ninth motor 8211 is started, the first connecting block 8214 is driven to slide back and forth along the second guide rail 8216 through the fourth screw rod 8212 and the fourth screw rod nut 8213, and the first connecting block 8214 then drives the connecting rod 8217, the pull rod 8218, and the hook head 8225 to move back and forth, so that the reagent card is taken out from the stacked card compartment 81 and sent to the transition card seat 8207. The incubation time in the filter card seat can be controlled by the residence time of the reagent card in the transition card seat 8207. If no intermediate incubation is required, the reagent card can be directly sent to the clamp 8306 of the continuous incubation unit 83; if intermediate incubation is required, assuming that the intermediate incubation time is △t1, the reagent card can be first incubated for the residence time t1 in the transition card seat 8207, and then sent to the clamp 8306 of the continuous incubation unit 83 for continued fixed time incubation. Assuming that the fixed incubation time is t2, the total incubation time of the reagent card is △t1 + t2, where △t1 is a variable, which can be determined according to different samples and reagents. Therefore, the present invention is suitable for both fixed-time incubation and variable-time incubation, and can be used for one-step method, delayed one-step method, and two-step method. It has a wide range of applications and good flexibility, which can save customers a lot of equipment investment and reduce the trouble of the tester's operation.

[0099] like Fig. 20 As shown, the incubation device 8 also includes a scanning unit 84, and the scanning unit 84 includes a fourth bottom plate 8401, an eleventh motor 8402, an eighth driving wheel 8403, an eighth belt 8404, an eighth passive wheel 8405, a belt clip 8407, and a fluorescent signal collector 8406. The fourth bottom plate 8401 is mounted on the top of the fourth frame 8301 by welding or bolting, and the eleventh motor 8402 is mounted on the fourth bottom plate 8401, and the output shaft of the eleventh motor 8402 is connected to the eighth driving wheel 8403, and the eighth driving wheel 8403 is connected to the eighth passive wheel 8405 through the eighth belt 8404, and the upper side of the eighth belt 8404 is fixedly installed with a belt clip 8407 by bolts and nuts, and the belt clip 8407 is fixedly connected to the fluorescent signal collector 8406 by countersunk screws or welding.

[0100] like Fig.21As shown, the incubation device 8 also includes a card-pulling unit 85, and the card-pulling unit 85 includes a twelfth motor 8501, a fifth screw rod 8502, a fifth screw rod nut 8503, a third connecting block 8504, a fifth slider 8505, a third guide rail 8506, and a lever 8507. The twelfth motor 8501 is fixedly mounted on the fourth base plate 8401 by bolts, the output shaft of the twelfth motor 8501 is connected to the fifth screw rod 8502 through a coupling, the fifth screw rod 8502 is provided with a fifth screw rod nut 8503 matching therewith, the fifth screw rod nut 8503 is fixedly connected to the third connecting block 8504 by countersunk screws, the bottom of the third connecting block 8504 is installed with a fifth slider 8505 by welding or countersunk screw connection, the fifth slider 8505 is located on the third guide rail 8506 and can slide back and forth along the third guide rail 8506, the third guide rail 8506 is fixedly mounted on the fourth base plate 8401 by countersunk screws, and the side of the third connecting block 8504 is fixedly mounted with a lever 8507 for removing the reagent card from the clamp 8306 by countersunk screws. The lever 8507 is a square rod, the upper end of which is welded to the third connecting block 8504 , and the lower end of which extends into the clamp 8306 of the continuous incubation unit 83 , and the lower end of which is slightly higher than the bottom surface of the clamp 8306 .

[0101] The above-mentioned embodiments are only preferred implementations of the present invention, and their descriptions are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A card-cage type multi-channel dry immunofluorescence detector, characterized in that: It includes a base, a test tube rack, a test tube, a first chassis, a puncture sampling device, a clamping and mixing synchronization device, a reagent cup transport device, and an incubation device. The front part of the base is provided with a test tube rack, and a plurality of test tubes are evenly placed on the test tube rack. The left side of the rear part of the base is provided with a clamping and mixing synchronization device. The right side of the rear part of the base is installed with a first chassis, and the puncture sampling device, the reagent cup transport device and the incubation device are arranged in the first chassis. The clamping mixing synchronization device includes a second chassis, a first fixed block, a second fixed block, a third fixed block, a fourth fixed block, a first driving wheel, a first passive wheel, a left guide column, a right guide column, a second driving wheel, a second passive wheel, a first motor, a second motor, a fourth motor, a first screw rod, a first belt, a second belt, a first rotating shaft, a first mounting plate, a first guide block, a second guide block, a left connecting plate, a right connecting plate, a left clamping plate, a right clamping plate, a left mounting seat, a right mounting seat, a first screw rod, a first screw rod nut, a first fixed plate, a mounting frame, a third driving wheel, a third passive wheel, a fourth driving wheel, a fourth passive wheel, a third Belt, fourth belt, second mounting plate, clamping device, sampling device, third guide column, third guide block, first screw nut; the first fixing block, second fixing block, third fixing block and fourth fixing block are installed on the second chassis, the first motor is also installed on the second chassis, the output shaft of the first motor is connected to the first driving wheel, the first driving wheel is connected to the first driven wheel through the first belt, the first driven wheel is connected to the second driving wheel through the first rotating shaft, and the second driving wheel is connected to the second driven wheel through the second belt; a left guide column is arranged between the second fixing block and the first fixing block, the left guide column The first guide block that can slide forward and backward along the left guide column is installed on the column, and the first guide block is connected to the left connecting plate, and the lower portion of the left connecting plate is installed with a left clamping plate that clamps the first belt, and the upper end of the left connecting plate is installed with a left mounting seat; a right guide column is provided between the fourth fixing block and the third fixing block, and the right guide column is installed with a second guide block that can slide forward and backward along the right guide column, and the second guide block is connected to the right connecting plate, and the lower portion of the right connecting plate is installed with a right clamping plate that clamps the second belt, and the upper end of the right connecting plate is installed with a right mounting seat; a first mounting plate is installed on one side of the left mounting seat, and a left mounting seat is installed on the first mounting plate A second motor is installed, the output shaft of the second motor is connected to the third driving wheel, the third driving wheel is connected to the third driven wheel through a third belt, the third driven wheel is connected to one end of the first screw rod, the other end of the first screw rod passes through the left mounting seat and is installed in the right mounting seat, the first screw rod is provided with a first screw rod nut, a third guide column is also installed between the left mounting seat and the right mounting seat, the third guide column is provided with a third guide block that can slide left and right along the third guide column, the third guide block and the first screw rod nut are both installed at the lower end of the first fixed plate, and the first fixed plate is fixedly connected to the clamping and mixing unit;A left baffle plate and a right baffle plate are installed on the left and right sides of the second mounting plate, a fourth motor is installed on the back of the second mounting plate, the output shaft of the fourth motor passes through the second mounting plate and is connected to the fourth driving wheel, the fourth driving wheel is connected to the fourth driven wheel through the fourth belt, a left guide rail is provided on the left side of the fourth belt, the left guide rail is installed on the front side of the second mounting plate, a left slider of sliding fit is installed on the left guide rail, the left slider is welded to the left connecting seat, a third clamping plate is installed on one side of the left connecting seat, and the third clamping plate clamps the left side of the fourth belt; a first base plate is welded on the left connecting seat, a stepping motor is installed on the upper surface of the first base plate, the output shaft of the stepping motor passes through the first base plate and is fixedly connected to the first cam located below the first base plate, a left swing arm and a right swing arm are respectively provided on the left and right sides of the first cam, a vertically downward pin is installed on the bottom surface of the first base plate, one end of the left swing arm and the right swing arm are both movably installed on the pin, the other end of the left swing arm is fixedly connected to the left clamping block, and a left clamping plate; the other end of the right swing arm is fixedly connected to the right clamping block, and the right clamping plate is installed on the right clamping block; the left and right swing arms are respectively welded with a left connecting block and a right connecting block, and the left and right connecting blocks are respectively provided with a first circular hole and a second circular hole, and the first circular hole and the second circular hole are connected by a first spring; a right guide rail is provided on the right side of the fourth belt, and the right guide rail is installed on the front side of the second mounting plate, and a right slider for sliding cooperation is installed on the right guide rail, and the right slider is connected to the right connecting seat The left guide rail and the right guide rail are fixedly connected, and both are parallel to the fourth belt; a fourth clamping plate is installed on the left side of the right connecting seat, and the fourth clamping plate clamps the right side of the fourth belt; the right connecting seat is in the shape of a channel steel, and is respectively composed of an upper transverse plate, a lower transverse plate and a first connecting plate, the upper transverse plate is provided with a first through hole, the lower transverse plate is provided with a second through hole, the liquid extraction needle passes upward through the second through hole and is connected to the needle tube located between the upper transverse plate and the lower transverse plate, the top of the needle tube passes through the first through hole and is connected to the micro pump, and the micro pump is connected to the liquid storage cylinder; The incubation device includes a stacked card warehouse, an intermediate incubation unit, and a continuous incubation unit. The intermediate incubation unit is arranged at the rear of the stacked card warehouse, and the continuous incubation unit is arranged at the rear of the intermediate incubation unit; the stacked card warehouse includes a box body, an upper card slot, a partition, a step block, a lower card slot, and a reagent card box. The box body is a rectangular parallelepiped with an opening at the front. The box body is composed of a left side plate, a right side plate, a lower side plate, an upper side plate and a back plate. A plurality of upper card slots are arranged on the upper side plate of the box body, and the upper card slots are evenly spaced by a plurality of partitions. A plurality of lower card slots are arranged on the lower side plate of the box body, and the lower card slots are evenly spaced by a plurality of step blocks. The distribution structure is that the upper card slot and the lower card slot correspond to each other one by one and are adapted to each other, the interval between the upper card slot and the lower card slot on the same vertical line forms a card slot cavity, and a reagent card box is movably installed in the card slot cavity; the intermediate incubation unit includes a third frame, an eighth motor, a third screw rod, a third screw rod nut, a second bottom plate, a third slider, a first guide rail, a vertical plate, a transition card seat, a fourth frame, a third bottom plate, a ninth motor, a fourth screw rod, a fourth screw rod nut, a first connecting block, a fourth slider, a second guide rail, a through hole, and a connecting rod; the right end of the third frame is installed with an eighth motor, the output shaft of the eighth motor is connected to the third screw rod, and the third screw rod A matching third screw nut is provided on the third screw nut, the third screw nut is connected to the second bottom plate, a third slider is installed on the bottom surface of the second bottom plate, and the third slider is movably installed on the first guide rail; a vertical plate is vertically installed on one side of the surface of the second bottom plate, and a transition card seat is installed on the upper part of the vertical plate; a third bottom plate is provided at the rear of the third frame, the third bottom plate is fixedly connected to the second bottom plate, a ninth motor is installed on the third bottom plate, the output shaft of the ninth motor is connected to the fourth screw, a matching fourth screw nut is provided on the fourth screw, the fourth screw nut is connected to the first connecting block, and a A fourth slider, wherein the fourth slider is movably mounted on the second guide rail and can slide back and forth along the second guide rail, the first connecting block is fixedly connected to the connecting rod, and the connecting rod is fixedly connected to the pull rod; the continuous incubation unit comprises a fourth frame, a tenth motor, a seventh driving wheel, a seventh belt, a seventh passive wheel, a clamp, and a second card slot, the tenth motor is mounted on the left end of the fourth frame, the output shaft of the tenth motor is connected to the seventh driving wheel, the seventh driving wheel is connected to the seventh passive wheel through the seventh belt, a plurality of evenly distributed clamps are mounted on the seventh belt, a second card slot is arranged in the clamp, and the size of the second card slot is adapted to the reagent card; The transition card seat comprises a left card plate, a right card plate, a second connecting block, a guide groove, a pull rod, a hook head, an oblique inlet and an oblique outlet. The left card plate and the right card plate are arranged in parallel and symmetrically. A long strip of gap is provided between the left card plate and the right card plate. A second connecting block is provided below the gap. The second connecting block is fixedly connected to the bottom of the left card plate and the right card plate respectively. A guide groove opening upward is provided on the second connecting block. A pull rod that can slide back and forth is provided in the guide groove. A hook head is provided at the front end of the pull rod. A left card plate with an opening facing the direction of the gap is provided on the inner side of the left card plate. A right card slot with an opening facing the gap direction is arranged on the inner side of the right card plate, the left card slot and the right card slot have the same width and height, the openings are opposite, and the left card slot and the right card slot are symmetrically distributed along the guide slot, the heights of the left card slot and the right card slot are adapted to the thickness of the reagent card, the distance between the left side surface of the left card slot and the right side surface of the right card slot is adapted to the width of the reagent card, the front end of the left card slot and the front end of the right card slot are both provided with an oblique entrance for introducing the reagent card, and the rear end of the left card slot and the rear end of the right card slot are both provided with an inclined oblique exit for withdrawing the reagent card.

2. The card-cage type multi-channel dry immunofluorescence detector according to claim 1, characterized in that: The reagent cup transport device comprises a first frame, a driving motor, a fifth driving wheel, a fifth driven wheel and a fifth belt, wherein the first frame is provided with a driving motor, and two ends of the first frame are provided with a fifth driving wheel and a fifth driven wheel respectively, the fifth driving wheel is connected to the output end of the driving motor through a rotating shaft, the fifth driven wheel is arranged at the other end of the first frame through a rotating shaft, the fifth belt is arranged on the fifth driving wheel and the fifth driven wheel and cooperates with the fifth driving wheel and the fifth driven wheel, a shell is arranged above the fifth belt, and the characteristic is that a slide rail is provided on the first frame, a first slider and a second slider matched with the slide rail are provided on the slide rail, a sample cup clamping unit is provided on the first slider and the second slider, and the sample cup clamping unit is fixedly connected to the fifth belt; The sample cup clamping unit includes a second connecting plate, a third connecting plate, a second fixing plate, a third fixing plate, a first clamping plate, a second clamping plate, a first arc groove, a second arc groove, a first connecting ear, a second connecting ear, a second spring, a square guide hole, a square guide rod, a circular guide hole, a circular guide rod, a baffle, a first bracket, a second bracket, and a collision block; the second fixing plate is vertically welded to the second connecting plate, the rear side of the second connecting plate is fixedly connected to the first sliding block, the bottom of the second connecting plate is fixedly connected to the fifth belt, the second fixing plate is provided with a first clamping plate, the first clamping plate is provided with a first arc groove, the The front end of the second fixing plate is provided with a first connecting ear; the third fixing plate is vertically welded to the third connecting plate, the rear side of the third connecting plate is fixedly connected to the second sliding block, the bottom of the third connecting plate is fixedly connected to the fifth belt, a second clamping plate is provided on one side of the third fixing plate, a second arc groove is provided on the surface of the second clamping plate opposite to the first clamping plate, the second arc groove on the second clamping plate cooperates with the first arc groove on the first clamping plate to clamp the sample cup, a second connecting ear is provided on the front end of the third fixing plate, the second connecting ear is connected to the right end of the second spring, and the left end of the second spring is connected to the first clamping plate; The first bracket is fixedly mounted on the left end of the first frame, a baffle plate vertically downward is mounted on the bracket, a circular guide rod is welded on the right side of the baffle plate, and a circular guide through hole matching the circular guide rod is provided on the first clamping plate; a square guide rod is provided on the side of the first clamping plate facing the second clamping plate, and a square guide through hole matching the square guide rod is provided on the second clamping plate; the second bracket is fixedly mounted on the right end of the first frame, and a collision block is mounted on the second bracket, and the position of the collision block corresponds to the position of the square guide rod on the first clamping plate; The first arc groove and the second arc groove are both semicircular grooves, a left rubber ring is pasted on the top of the first arc groove, and a right rubber ring is pasted on the top of the second arc groove, the left rubber ring and the right rubber ring are both semicircular, the positions of the left rubber ring and the right rubber ring correspond to each other, and the left rubber ring and the right rubber ring form a rubber ring when they are closed, and the inner diameter of the rubber ring is smaller than the outer diameter of the sample cup.

3. The card-cage type multi-channel dry immunofluorescence detector according to claim 2, characterized in that: The second clamping plate is provided with an accommodating cavity inside, the accommodating cavity is communicated with the second arc groove, a vibration shaking unit is provided in the accommodating cavity, the vibration shaking unit comprises a first shaking motor, an eccentric wheel, a V-shaped rod, a third bearing seat, a first rotating shaft, and a third spring, the third bearing seat and the first shaking motor are both installed at the bottom of the accommodating cavity, an eccentric wheel is installed on the output shaft of the first shaking motor, the bottom of the V-shaped rod is fixedly connected to the first rotating shaft, the first rotating shaft is installed in the bearing of the bearing seat, the V-shaped rod consists of a push rod and a rocker, the lower ends of the push rod and the rocker are welded and connected, the push rod and the rocker are V-shaped, and the angle between the push rod and the rocker is 90° to 135°, The push rod is located above the eccentric wheel, and an obliquely installed third spring is provided on the left side of the eccentric wheel. The upper end of the third spring is connected to the push rod, and the lower end of the third spring is fixedly installed on the bottom of the accommodating cavity. The upper end of the rocker is close to the second arc groove in the second clamping plate, and a wear-resistant rubber layer is pasted on the upper end of the rocker.

4. The card-cage type multi-channel dry immunofluorescence detector according to claim 1, characterized in that: The puncture sampling device includes a second frame, a sixth motor, a sixth driving wheel, a sixth driven wheel, a belt, a belt clamp, an upper guide rail, a lower guide rail, an upper slider, a lower slider, a mounting seat, a third mounting plate, a seventh motor, a second screw rod, a first guide column, a second guide column, a guide block, a sampling needle, a needle seat, a first guide hole, a second guide hole, a second screw rod nut, and a cleaning unit; The second frame is equipped with a sixth motor, the output shaft of the sixth motor is equipped with a sixth driving wheel, the sixth driving wheel is connected to the sixth driven wheel through a belt, the second frame is equipped with an upper guide rail and a lower guide rail, the upper guide rail is equipped with an upper slider, the lower guide rail is equipped with a lower slider, the upper slider and the lower slider can slide back and forth along the upper guide rail and the lower guide rail, the upper slider and the lower slider are both connected to the mounting seat, the mounting seat is equipped with a belt clamp, the belt clamp is located above the belt and clamps the upper belt, and the mounting seat is equipped with a third mounting plate that is perpendicular to the mounting seat; A seventh motor is installed on the third mounting plate, and an output shaft of the seventh motor is connected to the second screw rod. A first guide column and a second guide column are installed on the third mounting plate. A first guide hole, a second guide hole and a second screw nut are provided on the guide block. The first guide column and the second guide column respectively pass through the first guide hole and the second guide hole to form a sliding fit connection. A second screw nut is also installed on the guide block. The second screw rod forms a second screw rod transmission fit connection through the second screw nut. A vertically downward sampling needle is also installed at the front end of the guide block.

5. The card-cage type multi-channel dry immunofluorescence detector according to claim 4, characterized in that: A cleaning unit is provided at the lower part of the mounting seat, and the cleaning unit includes a connecting plate, a cleaning block, a center hole, a water inlet, a water outlet, a spiral groove, and a spiral slit; the connecting plate is fixedly installed at the bottom of the mounting seat, and the cleaning block is fixed to the connecting plate by screw connection or welding. A vertical center hole is provided at the center of the cleaning block, and the position of the center hole corresponds to the position of the sampling needle on the guide block. The sampling needle passes through the center hole and extends to the lower part of the cleaning block. A water inlet and a water outlet are provided on the side of the cleaning block. A spiral groove is provided inside the cleaning block, and the spiral groove is located on the outside of the center hole and spirals up around the center hole. The upper end of the spiral groove is connected to the water outlet, and the lower end of the spiral groove is connected to the water inlet. The spiral groove has a spiral slit on the side facing the center hole, and the spiral slit is communicated with the center hole, and the spiral slit spirals up around the center hole.

6. The card-cage type multi-channel dry immunofluorescence detector according to claim 1, characterized in that: The incubation device also includes a scanning unit, which includes a fourth base plate, an eleventh motor, an eighth driving wheel, an eighth belt, an eighth passive wheel, a belt clamp, and a fluorescent signal collector. The fourth base plate is installed on the top of a fourth frame, and the eleventh motor is installed on the fourth base plate. The output shaft of the eleventh motor is connected to the eighth driving wheel, and the eighth driving wheel is connected to the eighth passive wheel through an eighth belt. A belt clamp is installed on the eighth belt, and the belt clamp is fixedly connected to the fluorescent signal collector.

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