Anti-African swine fever virus (ASFV) and CD polyclonal micromolecular antibody, preparation method and application

An African swine fever virus and small molecule antibody technology, applied in the field of anti-African swine fever virus and CD polyclonal small molecule antibody and its preparation, and anti-African swine fever virus IgY antibody, which can solve the problem of inability to resist African swine fever infection and Diffusion, etc.

CN111690055APending Publication Date: 2020-09-22SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2020-09-22

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Abstract

The invention provides an anti-African swine fever virus (ASFV) IgY antibody, an anti-ASFV and CD composite IgY antibody, an anti-ASFV and CD polyclonal micromolecular antibody and composite antibodyoptimal combination application. Aiming to solve the problems that the ASFV takes porcine immune cells as target cells, destroys immune tissue of a host and causes vaccine failure, antigen proteins causing 'immunosuppression' and 'immune escape' and antigen proteins playing an important role in virus diffusion are used as target antigens, and the antigen proteins are inhibited and killed by adopting a composite antibody through humoral immunity, cellular immunity and ADCC action; the two bottlenecks of 'immunosuppression' and 'immune escape' are broken through, immune cells are protected and activated, and ASFVs and infected cells are removed. The composite antibody can be prepared into a biological medicine or a disinfectant for preventing and treating African swine fever, and can also beused as feed or a feed additive or an oral preparation component for blocking propagation of the ASFVs through oral cavities and digestive tracts.
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Description

technical field

[0001] The present invention relates to the technical field of antibody engineering and genetic engineering, more specifically, to an anti-African swine fever virus IgY antibody, anti-African swine fever virus and CD composite IgY antibody, anti-African swine fever virus and CD polyclonal small molecule Antibody and its preparation method and application. Background technique

[0002] African swine fever (African swine fever, ASF) is caused by African swine fever virus (African swine fever virus, ASFV), a kind of animal disease with high contagiousness, high morbidity and high mortality, which is harmful to my country and the whole world The pig industry is extremely dangerous.

[0003] African swine fever virus is highly resistant in tissues and environments, and there is no treatment to date. More importantly, African swine fever virus is an arbovirus with very unique DNA. It mainly infects pig mononuclear phagocytes as target cells, directly and indirectl...

Examples

Embodiment 1

[0090] Anti-African swine fever virus IgY antibody binding titer detection of five representative antigenic proteins including VP73, P54, P72, A238L, and CD2V.

[0091] Adopt VP73, P54, P72, A238L, CD2V respectively as detection antigen, use " ELISA " method (enzyme-linked immunoassay) to detect the anti-African swine fever virus IgY antibody titer prepared, the result is shown in the following table:

[0092]

[0093] Note: The concentration of the anti-ASFV IgY antibody solution in the test sample is 1mg / mL.

[0094] It can be seen from the above test results that the prepared anti-ASFV IgY antibodies have high antibody binding titers to representative VP73, P54, P72, P30, A238L, and CD2V protein antigens.

Embodiment 2

[0096] Anti-African swine fever virus and CD-IgY antibody binding titer detection of VP73, P54, P72, A238L, CD2V and other five representative antigenic proteins.

[0097] VP73, P54, P72, A238L, and CD2V were used as detection antigens respectively, and the titers of anti-African swine fever virus and CD-IgY antibodies prepared by "ELISA" method (enzyme-linked immunoassay) were detected, and the results are shown in the table below :

[0098]

[0099] Note: The concentration of the anti-ASF virus and CD-IgY antibody solution in the test sample is 1 mg / mL.

[0100] It can be seen from the above test results that the prepared anti-ASF virus and CD-IgY antibodies have high antibody binding titers to representative VP73, P54, P72, P30, A238L, and CD2V protein antigens.

Embodiment 3

[0102] Detection of Antibody Binding Titers of Anti-ASFV and CD-IgY Antibodies to CD. Take the CD3 antigen as an example.

[0103]Adopt CD3 as detection antigen, detect the anti-African swine fever virus and the antibody titer of CD-IgY antibody made with " ELISA " method (enzyme-linked immunoassay), the result is as shown in the table below:

[0104]

[0105] Note: the concentration of anti-ASF virus and CD-IgY antibody solution in the test sample is 1mg / mL.

[0106] As can be seen from the above test results, the prepared anti-ASF virus and CD-IgY antibodies have a high antibody binding titer to the CD3 antigen.