Magnetic Bead Washing System for Chemiluminescence Analyzers

Abstract

The present invention discloses a magnetic bead cleaning system for a chemiluminescence analyzer, comprising a solution containing part; a liquid extraction pipeline; a liquid injection pump, comprising a power source and at least two pump bodies driven by the power source, at least two third outlets are respectively connected to at least two pump bodies in a one-to-one correspondence; a liquid injection pipeline; and a liquid injection part, comprising a liquid injection needle connected to the other end of each liquid injection tube. The advantages of the present invention are that the liquid injection pump is a multi-channel plunger pump, and each pump body corresponds to a connecting branch pipe (i.e., a liquid extraction pipe) and a liquid injection pipe, so that each liquid injection needle can be controlled separately, effectively preventing the inconsistent liquid injection amount due to pipe resistance, thereby improving the liquid injection accuracy and the uniformity of the liquid injection amount; at the same time, it can also realize the cleaning of the entire pipeline, avoid crystallization due to the crystallization of the washing solution remaining in the pipeline, avoid the blockage of the pipeline, the blockage of the liquid injection needle and the blockage of the plunger pump, and further improve the liquid injection accuracy and the uniformity of the liquid injection amount.

Description

Technical Field

[0001] The invention relates to a chemiluminescence analyzer, in particular to a magnetic bead cleaning system used for the chemiluminescence analyzer. Background Art

[0002] The chemiluminescent labeling analyzer, also known as the chemiluminescent analyzer (CLIA), is a medical testing instrument that analyzes the human body by testing the patient's serum. During the test, a certain amount of patient serum and horseradish peroxide (HRP) are added to a white opaque microplate coated with antibodies on the solid phase. The molecules to be tested in the serum specifically bind to the conjugate of horseradish peroxidase and the antibodies on the solid phase carrier. The unreacted free components are separated and washed; then the luminescent substrate is added, and the free energy released by the chemiluminescence is used to excite the intermediate, returning from the ground state to the excited state, and the energy is released in the form of photons.

[0003] For a chemiluminescence analyzer, the liquid circuit system is an important component that affects the normal operation of the chemiluminescence analyzer, which mainly includes the reaction reagent injection system, the magnetic bead cleaning system, the luminescent substrate injection system and the waste liquid discharge system. The existing magnetic bead cleaning system uses a plunger pump (or diaphragm pump) to inject a certain volume of liquid into each reaction cup evenly through multiple branches (i.e., injection channels) to achieve the cleaning of the magnetic bead conjugate in the reaction cup. To ensure the uniformity of the injection, a solenoid valve is set on each branch. However, the above-mentioned magnetic bead cleaning system has many defects during the operation of the chemiluminescence analyzer, as follows:

[0004] (1) There are often process errors in the processing of each pipeline, so the flow resistance of each branch is not a constant value, resulting in poor injection stability and consistency between the branches and low injection accuracy.

[0005] (2) The cleaning solution delivered by the magnetic bead cleaning system is a crystalline cleaning solution, and the above-mentioned magnetic bead cleaning system is unable to flush the entire pipeline, resulting in blockage of the injection needle, further causing changes in the pipe resistance of each injection channel, reducing the injection accuracy, and a high instrument failure rate. Summary of the invention

[0006] The present invention aims to provide a magnetic bead cleaning system for a chemiluminescence analyzer.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] The magnetic bead cleaning system for a chemiluminescence analyzer of the present invention comprises

[0009] A solution containing portion, comprising at least one first container and at least one second container;

[0010] Liquid extraction line, including

[0011] a first pipeline having a first inlet and a first outlet, wherein the first inlet is connected to at least one of the first containers;

[0012] A liquid pump, connected and arranged on the first pipeline;

[0013] A second pipeline having a second inlet and a second outlet, wherein the second inlet is connected to at least one of the second containers; and

[0014] A connecting pipeline having a third inlet and at least two third outlets, wherein the first outlet and the second outlet are both connected to the third inlet;

[0015] The injection pump comprises a power source and at least two pump bodies driven by the power source, and at least two third outlets are respectively connected to the at least two pump bodies in a one-to-one correspondence;

[0016] The liquid injection pipeline includes a liquid injection pipe connected to each liquid injection port of the pump body and a first solenoid valve arranged on each of the liquid injection pipes, and at least two of the first solenoid valves constitute a first solenoid valve group; and

[0017] The liquid injection part includes a liquid injection needle which is connected to the other end of each liquid injection tube.

[0018] In a preferred embodiment of the present invention, the connecting pipeline includes a connecting main pipe having the third inlet and at least two connecting branch pipes each having the third outlet, and at least two connecting branch pipes are connected to the pump body suction port in a one-to-one correspondence;

[0019] A second solenoid valve is arranged on each connecting branch pipe, and at least the solenoid valves constitute a second solenoid valve group.

[0020] In a preferred embodiment of the present invention, the first outlet and the second outlet are connected to the third inlet via a third solenoid valve, and the third solenoid valve is a two-position three-way solenoid valve.

[0021] In another preferred embodiment of the present invention, the first outlet and the second outlet are connected to the third inlet via a multi-way connector, a fourth solenoid valve is provided on the first pipeline, and a fifth solenoid valve is provided on the second pipeline.

[0022] In a preferred embodiment of the present invention, at least two of the first solenoid valves are two-position three-way solenoid valves, and the first liquid discharge port of each first solenoid valve is respectively connected to the inlet of one of the liquid injection pipes.

[0023] In a preferred embodiment of the present invention, the magnetic bead cleaning system also includes a discharge portion, which includes a discharge pipe and a third container, wherein the liquid inlet end of the discharge pipe is respectively connected to the second discharge port of each of the first solenoid valves, and the discharge end thereof extends into the third container.

[0024] In a preferred embodiment of the present invention, a sixth solenoid valve is provided on the leakage pipe.

[0025] In a preferred embodiment of the present invention, a first liquid level sensor for detecting a liquid level signal is disposed in the first container, a second liquid level sensor for detecting a liquid level signal is disposed in the second container, and a third liquid level sensor for detecting a liquid level signal is disposed in the third container.

[0026] In a preferred embodiment of the present invention, the liquid pump is a diaphragm pump, a gear pump, a plunger pump or a magnetic pump.

[0027] In a preferred embodiment of the present invention, the power source is a motor, and the pump body is a plunger pump driven by the motor.

[0028] The advantage of the present invention is that the injection pump is a multi-channel plunger pump, and each pump body corresponds to a connecting branch pipe (i.e., a liquid extraction pipe) and an injection pipe, so that each injection needle can be controlled separately, effectively preventing inconsistent injection volume due to pipe obstruction, thereby improving the injection accuracy and uniformity of the injection volume.

[0029] The first solenoid valve of the present invention is a two-position three-way solenoid valve. The connectivity state of the first solenoid valve can be selectively adjusted during the injection and cleaning process, thereby achieving the cleaning of the liquid extraction pipeline, the injection pipeline, the plunger pump, the first solenoid group and the second solenoid valve group, avoiding crystallization of the washing liquid due to residue in the pipeline, avoiding pipeline blockage, injection needle blockage and plunger pump blockage, and further improving the injection accuracy and the uniformity of the injection amount. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a structural schematic diagram of implementation mode 1 of the present invention.

[0031] Figure 2 It is a circuit principle block diagram of implementation mode 1.

[0032] Figure 3 It is a structural schematic diagram of the second embodiment of the present invention.

[0033] Figure 4 It is a circuit principle block diagram of implementation mode 2.

[0034] Figure 5 It is a structural schematic diagram of implementation mode 3 of the present invention.

[0035] Figure 6 It is a circuit principle block diagram of the third new implementation mode of the present utility model. DETAILED DESCRIPTION

[0036] The following is a detailed description of an embodiment of the present invention in conjunction with the accompanying drawings. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0037] Implementation Method 1

[0038] like Figure 1 As shown, the magnetic bead cleaning system for a chemiluminescence analyzer of the present invention comprises a solution containing portion, a liquid extraction pipeline, a liquid injection pump 100, a liquid injection pipeline, a liquid injection portion and a flow discharge portion;

[0039] The injection pump 100 is a multi-channel plunger pump (i.e., a plunger pump driven by the same motor), and the pump body of each plunger pump has a liquid suction port and a liquid injection port;

[0040] The injection part includes five injection needles 200 (of course, the number of injection needles 200 can be any number within the range of 2 to 15 according to actual conditions);

[0041] The solution containing part includes a first container 300 for containing a cleaning solution (such as purified water) and a second container 400 for containing a crystalline washing solution (such as a BF washing solution);

[0042] The liquid extraction pipeline includes a first pipeline having a first inlet and a first outlet (i.e., a first liquid extraction pipe 501), and a second pipeline having a second inlet and a second outlet (i.e., a second liquid extraction pipe 502). The first inlet of the first liquid extraction pipe 501 is inserted into the bottom of the first container 300, and the second inlet of the second liquid extraction pipe 502 is inserted into the bottom of the second container 400. The first liquid extraction pipe 501 is also provided with a liquid extraction pump 503 (the liquid extraction pump 503 is a diaphragm pump, a gear pump, a plunger pump or a magnetic pump) so as to pump the purified water in the first container 300 to the liquid injection pipeline and the liquid injection needle 200 to achieve flushing.

[0043] The liquid extraction pipeline also includes a connecting pipeline connecting the first liquid extraction pipe 501 and the second liquid extraction pipe 502 together, and the connecting pipeline includes a connecting main pipe 504 with a third inlet and five connecting branch pipes 505 of the same length. One end of the connecting main pipe 504 is the third inlet, and the first inlet, the second inlet and the third inlet are connected together through a third solenoid valve 506 (the third solenoid valve 506 is a two-position three-way solenoid valve); one end of each connecting branch pipe 505 is connected to the liquid discharge end of the connecting main pipe 504 and the other end is connected to the liquid suction port of a plunger pump body, and each connecting branch pipe 505 is provided with a second solenoid valve 507 (the second solenoid valve is installed at the same position on each connecting branch pipe), and the five second solenoid valves 507 constitute a second solenoid valve group;

[0044] The liquid injection pipeline includes a liquid injection tube 601 connected to the liquid injection port of each plunger pump body (that is, there are five liquid injection tubes 601, and the lengths are the same), each liquid injection tube 601 is provided with a liquid injection needle 200 at the liquid discharge port, and each liquid injection tube 601 is provided with a first solenoid valve 602 (the first solenoid valve is installed at the same position on each liquid injection tube), and the five first solenoid valves 602 constitute a first solenoid valve group;

[0045] The discharge part includes a discharge pipe 701 and a third container 702. The liquid inlet end of the discharge pipe 701 is respectively connected to the second liquid discharge port of each first solenoid valve 602, and the liquid discharge end thereof extends into the third container 702. The discharge pipe 701 is provided with a sixth solenoid valve 703 (a two-position solenoid valve) for controlling its on-off state.

[0046] like Figure 1 As shown, the first container 300 is provided with a first liquid level sensor 301 for detecting its liquid level signal, the second container 400 is provided with a second liquid level sensor 401 for detecting its liquid level signal, and the third container 702 is provided with a third liquid level sensor 704 for detecting its liquid level signal.

[0047] like Figure 2 As shown, in order to realize automatic control, the signal output ends of the first liquid level sensor 301, the second liquid level sensor 401 and the third liquid level sensor 704 are electrically connected to the signal input end of the control system of the chemiluminescence instrument, and the first liquid level sensor 301, the second liquid level sensor 401 and the third liquid level sensor 704 respectively transmit the detected liquid level signals to the control system in real time, and the control system processes the received liquid level signals and controls the start and stop of the liquid pump 503 and the motor, as well as the opening and closing of the first solenoid valve 602, the second solenoid valve 507, the third solenoid valve 506 and the sixth solenoid valve 703.

[0048] When the liquid level signal detected by the first liquid level sensor 301 reaches the preset lower limit liquid level, the control system issues an alarm to remind the staff to add purified water; when the liquid level signal detected by the second liquid level sensor 401 reaches the preset lower limit liquid level, the control system issues an alarm to remind the staff to add crystalline washing solution; when the liquid level signal detected by the second liquid level sensor 401 reaches the preset upper limit liquid level, the control system issues an alarm to remind the staff to pour out the solution in the third container 702.

[0049] The specific working principle and working process of the present invention are briefly described as follows:

[0050] During the operation of the chemiluminescence analyzer, when the magnetic bead sample needs to be injected and cleaned: the control system switches all the first solenoid valves 602 to a state connected to the drain pipe 701, the sixth solenoid valve 703 is in a closed state, the second solenoid valves 507 are all in an open state, and the third solenoid valve 506 is switched to a state connected to the second liquid extraction pipe 502;

[0051] Then the control system sends a liquid suction action instruction to the motor, and multiple plunger pumps suck liquid at the same time (the volume of washing liquid in the pump chambers of multiple plunger pumps is the same). After the suction is completed, the second electromagnetic valve group is closed; if the reaction cup on the reaction disk rotates to the bottom of the injection needle 200, the first electromagnetic valve 602 corresponding to the injection needle 200 is switched to the state of being connected to the injection needle 200 (while the first electromagnetic valve 602 without a reaction cup below is still in a state of being connected to the drainage pipe 701), and the sixth electromagnetic valve 703 is also in an open state, the control system sends a liquid discharge instruction to the motor, and the motor reverses the plunger pump to inject liquid, thereby achieving liquid injection and ensuring that the washing liquid is accurately injected into the reaction cup below; and the washing liquid flowing into the remaining injection pipes 601 flows into the third container 702 through the drainage pipe 701;

[0052] During the injection process, when more reaction cups rotate to the bottom of the injection needle 200, the control system controls the first solenoid valve 602 corresponding to each reaction cup to switch to a state of connection with the injection needle 200, while the first solenoid valve 602 corresponding to no reaction cup below is still in a state of connection with the drain pipe 701, thereby achieving simultaneous injection of one, two, or any three, or any four or more reaction cups.

[0053] After the injection is completed, the injection pipeline and the injection needle 200 need to be flushed:

[0054] The control system controls the third solenoid valve 506 to switch to a state of connection with the first liquid extraction pipe 501, the first solenoid valve group to switch to a state of connection with the drain pipe 701, the second solenoid valve group and the sixth solenoid valve 703 are opened, the liquid extraction pump 503 stops working after continuously extracting liquid for a preset time, the control system controls the first solenoid valve group to switch to a state of connection with the injection needle 200, the second solenoid valve group and the sixth solenoid valve 703 are closed, the third solenoid valve 506 is switched to a state of connection with the second container 400, and the pipeline cleaning (including the liquid extraction pipeline, the second solenoid valve group, the first solenoid valve group and the injection main pipe) is completed.

[0055] The control system controls the third solenoid valve 506 to switch to a state of communication with the first container 300, opens the second solenoid valve group, and switches the first solenoid valve group to a state of communication with the drain pipe 701. The liquid pump 503 continues to work for a preset time, and the plunger pump sucks liquid. Purified water can clean the pump chamber of the plunger pump. The control system closes the second solenoid valve group. When it is detected that the reaction cup rotates to the bottom of one (or two, or three or more) injection needles 200, the control system controls the first solenoid valve 602 corresponding to the reaction cup to switch to a state of communication with the injection needle 200 (the first solenoid valve 602 without a reaction cup below is still in a state of communication with the drain pipe 701), and the sixth solenoid valve 703 is also in an open state, the plunger pump injects liquid, and then the first solenoid valve 602 is reset to a state of communication with the drain pipe 701, and the sixth solenoid valve 703 is closed to achieve the cleaning of one (or two, three, or even more) injection needles 200. Repeat this operation process until the cleaning operation of all injection needles 200 is completed. And in the process of repeated liquid pumping and cleaning, the pump cavity of the plunger pump can be cleaned multiple times to ensure the cleaning effect.

[0056] The magnetic bead cleaning system of the present invention can continuously clean the liquid extraction pipeline (excluding the second liquid extraction pipe 502), the second solenoid valve group on the liquid extraction pipeline, the injection pipeline, the plunger pump, the first solenoid valve group on the injection pipeline and the injection needle 200 at a large flow rate, and has a good cleaning effect, effectively reducing the probability of crystallization blockage in the entire system.

[0057] Implementation Method 2

[0058] The difference between this embodiment and the first embodiment is that the connection method between the first liquid extraction pipe 501, the second liquid extraction pipe 502 and the connecting pipeline is different: Figure 3-4As shown, the first liquid extraction pipe 501 and the second liquid extraction pipe 502 are connected to the connecting main pipe 504 of the connecting pipeline through a three-way connector 508, and a fourth electromagnetic valve 509 is provided on the first liquid extraction pipe 501, and a fifth electromagnetic valve 510 is provided on the second liquid extraction pipe 502. During operation, if liquid injection is required, the control system controls the fourth electromagnetic valve 509 to be in a disconnected state and the fifth electromagnetic valve 510 to be in a connected state; if purified water is required for cleaning, the control system controls the fourth electromagnetic valve 509 to be in an open state and the fifth electromagnetic valve 510 to be in a disconnected state.

[0059] Implementation Method 3

[0060] The difference between this embodiment and the first embodiment is only the number of first containers 300. Figure 5-6 As shown, there are two first containers 300 (of course, three can also be used), and the first liquid extraction pipe 501 has two liquid extraction branches respectively connected to the first container 300 and a liquid extraction main pipe connected to the other ends of the two liquid extraction branches. The liquid extraction main pipe is connected to the two liquid extraction branches through the seventh solenoid valve 511 (a two-position three-way solenoid valve). The staff can replace a bottle of purified water in time after it is used up. At the same time, the control system switches the state of the seventh solenoid valve 511 to ensure that there is always water supply during cleaning, so as to achieve continuous cleaning and effectively avoid the pause caused by insufficient purified water.

[0061] During actual installation, two second containers 400 that can be used alternately can be provided according to actual needs to achieve continuous liquid injection.

[0062] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the direction of a certain posture (such as the attached Figure 1 The relative position relationship and movement of the components under the control of the controller (as shown in the figure) and the like, if the specific posture changes, the directional indication will also change accordingly. In the present invention, unless otherwise clearly specified and limited, the terms "connection" and "fixed" should be understood in a broad sense. For example, "fixed" can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0063] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text is to include three parallel solutions. Taking "A and / or B as an example", it includes solution A, or solution B, or solutions that satisfy both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

Claims

1. A magnetic bead cleaning system for a chemiluminescence analyzer, characterized in that: include A solution containing portion, comprising at least one first container and at least one second container; Liquid extraction line, including a first pipeline having a first inlet and a first outlet, wherein the first inlet is connected to at least one of the first containers; A liquid pump, connected and arranged on the first pipeline; A second pipeline having a second inlet and a second outlet, wherein the second inlet is connected to at least one of the second containers; and A connecting pipeline having a third inlet and at least two third outlets, wherein the first outlet and the second outlet are both connected to the third inlet; The injection pump comprises a power source and at least two pump bodies driven by the power source, and at least two third outlets are respectively connected to the at least two pump bodies in a one-to-one correspondence; The liquid injection pipeline includes a liquid injection pipe connected to each liquid injection port of the pump body and a first solenoid valve arranged on each of the liquid injection pipes, and at least two of the first solenoid valves constitute a first solenoid valve group; as well as The liquid injection part includes a liquid injection needle connected to the other end of each liquid injection tube; The connecting pipeline includes a connecting main pipe having the third inlet and at least two connecting branches each having the third outlet, and at least two connecting branches are connected to the pump body suction port in a one-to-one correspondence; each connecting branch is provided with a second solenoid valve, and at least two of the second solenoid valves constitute a second solenoid valve group; At least two of the first solenoid valves are two-position three-way solenoid valves, and the first liquid discharge port of each first solenoid valve is respectively connected to the inlet of one of the injection pipes; The magnetic bead cleaning system also includes a drainage part, which includes a drainage pipe and a third container. The liquid inlet end of the drainage pipe is respectively connected to the second liquid discharge port of each first solenoid valve, and the liquid discharge end thereof extends into the third container.

2. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The first outlet and the second outlet are connected to the third inlet through a third solenoid valve, and the third solenoid valve is a two-position three-way solenoid valve.

3. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The first outlet and the second outlet are connected to the third inlet via a multi-way connector, a fourth solenoid valve is arranged on the first pipeline, and a fifth solenoid valve is arranged on the second pipeline.

4. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The leakage pipe is provided with a sixth solenoid valve.

5. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The first container is provided with a first liquid level sensor for detecting a liquid level signal thereof, the second container is provided with a second liquid level sensor for detecting a liquid level signal thereof, and the third container is provided with a third liquid level sensor for detecting a liquid level signal thereof.

6. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The liquid pump is a diaphragm pump, a gear pump, a plunger pump or a magnetic pump.

7. The magnetic bead cleaning system for a chemiluminescence analyzer according to claim 1, characterized in that: The power source is a motor, and the pump body is a plunger pump driven by the motor.

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