A cell preparation device

Abstract

The present invention discloses a cell preparation device, and relates to the technical field of cell preparation. It includes an operating table, and a slide loading component, a circulating transmission component, a sedimentation bin placement component, a sedimentation bin removal component, and a slide removal component arranged on the operating table. The operating table is also provided with a liquid inlet component, a liquid extraction component, and a liquid adding component, wherein the circulating transmission component includes a stage, the slide loading component is used to load the slide on the stage, and the sedimentation bin placement component is used to place the sedimentation bin on the slide; the liquid inlet component injects the sample liquid into the sedimentation bin, and when the stage flows to different positions, the liquid extraction component extracts the supernatant formed in the sedimentation bin; the liquid adding component is used to sequentially add separation liquid, buffer solution, staining liquid, rinsing liquid, and transparent liquid into the sedimentation bin, the sedimentation bin removal component removes the sedimentation bin, and the slide removal component removes and collects the slide after preparation. It can improve the automation degree of cell preparation and improve the preparation efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of cell preparation, and in particular to a cell preparation device. Background Art

[0002] Pathology liquid-based thin-layer cell detection technology is an effective means of screening cervical cancer, and natural sedimentation cell preparation technology is its cutting-edge field. The basic principle of natural sedimentation cell preparation technology is to use gradient separation and liquid-based technology to transfer representative cells to a slide by natural sedimentation, and then stain them to form a cell sheet with natural cell morphology, uniform distribution, single-layer tiling, clean background, distinct color, and clear structure, so that researchers can observe and study clearly.

[0003] In the prior art, it is necessary to manually pour the cell solution in the test tube into the cell smearer before making the slide. After the cells naturally settle in the cleaning solution, the cells are adhered to the slide, that is, a slide with cells is made. Then the cells are cleaned with cleaning solution, and the cleaning solution is extracted. The whole process requires manual preparation of multiple slides with cells, and then these slides are uniformly placed in the above-mentioned liquid-based cell automatic staining machine for staining. The operation process is relatively complicated, the labor intensity is high, and the production efficiency is low. Summary of the invention

[0004] The object of the present invention is to provide a cell preparation device, which can improve the automation level of cell preparation, reduce labor intensity, and improve preparation efficiency.

[0005] The embodiment of the present invention is achieved as follows:

[0006] The embodiment of the present application provides a cell preparation device, including an operating table, and a glass slide loading component, a circulating transmission component, a sedimentation bin placement component, a sedimentation bin removal component, and a glass slide removal component arranged on the operating table, and a liquid inlet component, a liquid extraction component, and a liquid adding component are also arranged on the operating table, wherein the circulating transmission component includes a stage, the glass slide loading component is used to load the glass slide on the stage, and the sedimentation bin placement component is used to place the sedimentation bin on the glass slide;

[0007] The liquid inlet component injects the sample liquid into the sedimentation chamber. When the stage moves to different positions, the liquid extraction component extracts the supernatant formed in the sedimentation chamber. The liquid adding component is used to add separation liquid, buffer solution, staining liquid, rinse liquid and transparent liquid into the sedimentation chamber in sequence. The sedimentation chamber removal component removes the sedimentation chamber, and the slide removal component removes and collects the slides after preparation.

[0008] Optionally, the slide loading assembly includes a transmission assembly and a first silo cooperating with the transmission assembly, the first silo is used to store the slides; the transmission assembly includes a first motor and a first lead screw drivingly connected to the first motor, the first lead screw is provided with a first slider, the first slider is slidably connected to the first slide rail, the first slider is also provided with a push plate, the push plate is slidably connected to the second slide rail, the push plate is used to load the slide onto the stage and push the stage to move a first preset distance.

[0009] Optionally, the circulating transmission component also includes a closed sliding track and at least one set of propulsion components cooperating with the sliding track, the propulsion component includes a second motor and a second lead screw drivingly connected to the second motor, a second slider is provided on the second lead screw, the second slider is slidably connected to the third slide rail, and a push rod is also provided on the slider, which is used to push the worktable a second preset distance.

[0010] Optionally, the sedimentation bin placement assembly includes a discharge assembly and a barrel cooperating with the discharge assembly, the barrel is used to stack the sedimentation bins, the bottom of the barrel has a notch to expose the sedimentation bin, the discharge assembly includes a first support frame, and a third motor arranged on the first support frame, the third motor is drivingly connected to the third lead screw, the third lead screw is provided with a third slider, the third slider is slidably connected to the first support frame, the third slider is provided with a first manipulator, and the first manipulator is used to grab the sedimentation bin.

[0011] Optionally, the sedimentation bin removal assembly includes a bracket and a first motor arranged on the bracket, the first motor is transmission-connected to the nut screw, a sliding block is arranged on the nut screw, a first support plate is arranged on the sliding block, a second motor is arranged on the first support plate, the second motor is connected to the second support plate, a second manipulator is arranged on the second support plate, and the second manipulator is used to grab the sedimentation bin.

[0012] Optionally, the slide removal assembly includes a material removal assembly and a material box cooperating with the material removal assembly, wherein a plurality of protrusions are arranged at intervals in the material box so that the slides can be placed at intervals in the material box; the material removal assembly includes a second support frame, and a first drive motor arranged on the second support frame, the first drive motor is drivingly connected to the first screw rod, a first sliding block is arranged on the first screw rod, the first sliding block is slidably connected to the second support frame, a fixed plate is arranged on the first sliding block, a second drive motor is arranged on the fixed plate, the second drive motor is drivingly connected to the second screw rod, a second sliding block is arranged on the second screw rod, the second sliding block is slidably connected to the fixed plate, a third manipulator is arranged on the second sliding block, and the third manipulator is used to grab the slide.

[0013] Optionally, the liquid inlet assembly includes a first mounting plate and a second mounting plate that are spaced apart, at least one set of translation assemblies is arranged on the first mounting plate, the second mounting plate is used to place a sample rack, the translation assembly is used to push the sample rack to translate on the first mounting plate, the sample rack is used to place a sample bottle containing sample liquid, a sampling assembly is also arranged on the first mounting plate, and a sample injection assembly connected to the sampling assembly is arranged on the operating table for injecting the extracted sample liquid into the sedimentation chamber.

[0014] Optionally, the translation assembly includes a rotating motor, a synchronous wheel is provided at the output end of the rotating motor, and the synchronous wheel is connected to the idle wheel through a synchronous belt; the translation assembly also includes a linear slide rail, a connecting plate is slidably provided on the linear slide rail, the connecting plate is fixedly connected to the synchronous belt, and a pushing assembly is provided on the connecting plate for pushing the sample rack.

[0015] Optionally, the sampling assembly includes a first rotary driver, a first turntable connected to the first rotary driver, a first bracket arranged on the first turntable, and a first sampling needle and a second sampling needle respectively arranged on both sides of the first bracket, the first sampling needle has a sample bottle corresponding to its lower portion, a cleaning tank for containing a cleaning solution corresponding to its lower portion, and the first rotary driver drives the first turntable to rotate so that the position of the first sampling needle and the position of the second sampling needle are interchanged;

[0016] The injection assembly includes a second rotary driver, a second bracket, and a first injection needle and a second injection needle respectively arranged on both sides of the second bracket. The lower side of the first injection needle corresponds to the sedimentation bin, and a recovery tank for recovering the cleaning liquid is arranged correspondingly below the second injection needle. The tail end of the first injection needle is connected to the tail end of the first sampling needle through a pipeline, and the tail end of the second injection needle is connected to the tail end of the second sampling needle through a pipeline. The driving end of the second rotary driver is transmission-connected to the second bracket, and the second rotary driver drives the second bracket to rotate so that the position of the first injection needle and the second injection needle are interchangeable.

[0017] Optionally, the cell preparation device also includes a coding component, which includes an information acquisition component, an information processing component and a laser coder that are electrically connected to each other, the information acquisition component is arranged on one side of the sample rack, and the laser coder is located above the stage; wherein the information acquisition component is used to transmit the sample information of the collected sample bottle to the information processing component, and the information processing component is used to control the laser coder to code the slide according to the sample information.

[0018] Optionally, the liquid extraction component includes a liquid extraction pump, a liquid extraction pipeline and a waste liquid bottle, and the liquid extraction pump is arranged on the liquid extraction pipeline, and is used to extract the supernatant into the waste liquid bottle through the liquid extraction pipeline.

[0019] Optionally, the liquid adding assembly includes at least one liquid supply bottle, a liquid supply pipeline connected to the liquid supply bottle, and a liquid supply pump is provided on the liquid supply pipeline for pumping the liquid in the liquid supply bottle into the sedimentation bin through the liquid supply pipeline.

[0020] The beneficial effects of the embodiments of the present invention include:

[0021] The film-making device provided by the embodiment of the present invention can circulate the stage through the circulating transmission component, and since the stage on the circulating transmission component includes multiple stages, when one of the stages corresponds to the slide loading component, the slide loading component loads the slide on the stage. After the slide loading is completed, the sedimentation chamber is placed on the slide through the sedimentation chamber placement component, so that the required matching relationship is formed between the sedimentation chamber and the slide, which is convenient for subsequent sample addition. After the sedimentation chamber is placed, the separation liquid can be added to the sedimentation chamber through the liquid adding component, and the sample liquid can be injected into the sedimentation chamber through the liquid inlet component, so that the cells in the sample liquid injected into the sedimentation chamber are settled to the surface of the slide. After the cell sedimentation, with the circulation of the stage, until it corresponds to the liquid extraction component, the supernatant formed in the sedimentation chamber is extracted by the liquid extraction component to achieve the purpose of removing interfering components. After the supernatant is extracted, a buffer solution is added through the liquid adding component to maintain the pH value of the experimental system and ensure the effectiveness of the film-making. The cells that have settled on the surface of the slide are stained by adding a staining liquid into the sedimentation bin through the liquid adding component. After being stained, the flushing liquid and the transparent liquid are added again through the liquid adding component. The flushing liquid can effectively decompose and remove impurities such as red blood cells and mucus clumps mixed into the cell sample when the material is collected, so that the liquid-based thin layer of cells can be clearly read under the microscope. The transparent liquid can make the stained cells transparent for easy sealing. After completing the above steps, the sedimentation bin is removed by the sedimentation bin removal component. After the sedimentation bin is removed, the slide is removed and collected through the slide removal component. The whole process does not require manual intervention, which can improve the degree of automation of cell preparation, reduce labor intensity, and improve production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0023] Figure 1 A schematic diagram of the structure of a cell preparation device provided in an embodiment of the present invention;

[0024] Figure 2 A schematic diagram of the structure of a slide loading assembly provided in an embodiment of the present invention;

[0025] Figure 3 A schematic diagram of the structure of a circulating transmission assembly provided in an embodiment of the present invention;

[0026] Figure 4 A schematic diagram of the structure of a sedimentation bin placement assembly provided in an embodiment of the present invention;

[0027] Figure 5 A schematic diagram of the structure of a sedimentation bin removal assembly provided in an embodiment of the present invention;

[0028] Figure 6 A schematic diagram of the structure of a glass slide removal assembly provided in an embodiment of the present invention;

[0029] Figure 7 A schematic diagram of the structure of a liquid inlet assembly provided in an embodiment of the present invention;

[0030] Figure 8 One of the structural schematic diagrams of the translation assembly provided in an embodiment of the present invention;

[0031] Fig. 9 A second structural schematic diagram of a translation assembly provided in an embodiment of the present invention;

[0032] Fig.10 A third structural schematic diagram of a translation assembly provided in an embodiment of the present invention;

[0033] Fig.11 A schematic diagram of the structure of a sampling component provided in an embodiment of the present invention;

[0034] Fig.12 A schematic diagram of the structure of a sample injection assembly provided in an embodiment of the present invention;

[0035] Fig.13 A schematic diagram of the structure of a coding component provided by an embodiment of the present invention;

[0036] Fig.14 A schematic diagram of the structure of a liquid extraction assembly provided in an embodiment of the present invention;

[0037] Fig.15 A schematic structural diagram of a liquid adding assembly provided in an embodiment of the present invention.

[0038] Icons: 100-cell preparation device; 105-operating table; 107-slide; 110-slide loading assembly; 111-first silo; 112-first motor; 113-first lead screw; 114-first slider; 115-first slide rail; 116-push plate; 117-second slide rail; 119-base; 120-circulating transmission assembly; 121-stage; 122-closed slide rail; 123-second motor; 124-second lead screw; 125-second slider; 126-third slide rail; 127-push rod; 128-sedimentation bin; 129-propelling assembly; 1 30-settling bin placement assembly; 131-barrel; 132-first support frame; 133-third motor; 134-third lead screw; 135-third slider; 136-first manipulator; 140-settling bin removal assembly; 141-bracket; 142-first motor; 143-nut lead screw; 144-sliding block; 145-first support plate; 146-second motor; 147-second support plate; 148-second manipulator; 150-slide removal assembly; 151-material box; 152-second support frame; 153-first drive motor; 154-first lead screw; 15 5-first sliding block; 156-fixed plate; 157-second driving motor; 159-second sliding block; 1592-third manipulator; 160-liquid inlet assembly; 161-first mounting plate; 162-second mounting plate; 163-translation assembly; 1631-rotation motor; 1632-synchronous wheel; 1633-synchronous belt; 1634-idle wheel; 1635-linear slide rail; 1636-connecting plate; 1637-pushing assembly; 164-sample rack; 165-sample bottle; 166-sampling assembly; 1661-first rotary drive; 1662-first turntable; 16 63-first bracket; 1664-first sampling needle; 1665-second sampling needle; 1666-cleaning tank; 167-injection assembly; 1671-second rotary drive; 1672-second bracket; 1673-first injection needle; 1674-second injection needle; 170-coding assembly; 172-information acquisition assembly; 174-information processing assembly; 176-laser coder; 180-liquid extraction assembly; 182-liquid extraction pump; 184-liquid extraction pipeline; 186-waste liquid bottle; 190-liquid addition assembly; 192-liquid supply bottle; 194-liquid supply pipeline; 196-liquid supply pump. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0040] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0042] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0043] Please refer to Figure 1 The present embodiment provides a cell preparation device 100, comprising an operating table 105, and a glass slide loading assembly 110, a circulating transmission assembly 120, a sedimentation chamber placement assembly 130, a sedimentation chamber removal assembly 140, and a glass slide removal assembly 150 arranged on the operating table 105, and the operating table 105 is also provided with a liquid inlet assembly 160, a liquid extraction assembly 180 and a liquid adding assembly 190, wherein the circulating transmission assembly 120 includes a stage 121, and the glass slide loading assembly 110 is used to load the glass slide 107 on the stage 121. On the stage 121, the sedimentation chamber placement component 130 is used to place the sedimentation chamber 128 on the slide 107; the liquid inlet component 160 injects the sample liquid into the sedimentation chamber 128, and when the stage 121 flows to different positions, the liquid extraction component 180 extracts the supernatant formed in the sedimentation chamber 128; the liquid adding component 190 is used to add separation liquid, staining liquid or transparent liquid to the sedimentation chamber 128 in sequence, the sedimentation chamber removal component 140 removes the sedimentation chamber 128, and the slide removal component 150 removes and collects the prepared slide 107.

[0044] Specifically, the circulating transmission component 120 includes a loading platform 121, and enables the loading platform 121 to circulate between different workstations, so that the loading platform 121 corresponds to the slide loading component 110, the sedimentation bin placement component 130, the sedimentation bin removal component 140, and the slide removal component 150, respectively, so that the corresponding components can perform related operations.

[0045] The sample fluid in the embodiments of the present application can be a cervical scraping, pleural effusion, puncture fluid, sputum specimen, etc. By using liquid-based cytology, the exfoliated cytology specimen that is difficult to handle by traditional methods is placed in an intermediate liquid to remove interfering components such as blood and mucus that affect the diagnosis, thereby achieving the purpose of improving the diagnosis rate.

[0046] The slide making device provided in the embodiment of the present invention can circulate the stage 121 through the circulating transmission component 120. Since the stage 121 on the circulating transmission component 120 includes multiple stages, when one of the stages 121 corresponds to the slide loading component 110, the slide loading component 110 loads the slide 107 on the stage 121. After the slide 107 is loaded, the sedimentation chamber 128 is placed on the slide 107 through the sedimentation chamber placement component 130, so that the required matching relationship is formed between the sedimentation chamber 128 and the slide 107, which is convenient for subsequent sample addition. After the sedimentation chamber 128 is placed, the separation liquid can be added to the sedimentation chamber 128 through the liquid adding component 190, and the sample liquid can be injected into the sedimentation chamber 128 through the liquid inlet component 160, so that the cells in the sample liquid injected into the sedimentation chamber 128 are settled on the surface of the slide 107. After the cells settle, as the stage 121 circulates, until it corresponds to the liquid extraction component 180, the supernatant formed in the sedimentation chamber 128 is extracted by the liquid extraction component 180 to achieve the purpose of removing interfering components. After the supernatant is extracted, a buffer is added through the liquid addition component 190 to maintain the pH value of the experimental system and ensure the effectiveness of the film preparation. The cells that settle on the surface of the slide 107 are stained by adding a staining liquid to the sedimentation chamber 128 through the liquid addition component 190. After being stained, a flushing liquid and a transparent liquid are added again through the liquid addition component 190. The flushing liquid can effectively decompose and remove impurities such as red blood cells and mucus clumps mixed into the cell sample during the sampling, so that the liquid-based thin layer of cells is clear when the background is read under the microscope, and the transparent liquid can make the stained cells transparent for easy sealing. After completing the above steps, the sedimentation chamber 128 is taken out through the sedimentation chamber removal component 140. After taking out the sedimentation chamber 128, the prepared slide 107 is taken out and collected through the slide removal component 150. The whole process does not require manual intervention, which can improve the degree of automation of cell preparation, reduce labor intensity, and improve preparation efficiency.

[0047] like Figure 2As shown, the slide loading assembly 110 includes a transmission assembly and a first silo 111 coordinated with the transmission assembly, the first silo 111 being used to store the slides 107; the transmission assembly includes a first motor 112 and a first lead screw 113 drivingly connected to the first motor 112, a first slider 114 being provided on the first lead screw 113, the first slider 114 being slidably connected to the first slide rail 115, a push plate 116 being further provided on the first slider 114, the push plate 116 being slidably connected to the second slide rail 117, the push plate 116 being used to load the slide 107 onto the stage 121, and to push the stage 121 to move a first preset distance.

[0048] Specifically, the first material bin 111 can be placed on the base 119 and can be detachably connected to the base 119. When the glass slides 107 in the first material bin 111 are used up, the first material bin 111 can be replaced in time, which is conducive to improving the continuity and efficiency of film production. The first material bin 111 is provided with a via hole, which can be a rectangular via hole. In this way, when the first motor 112 drives the push plate 116 to move in the direction of the first material bin 111, it can drive the glass slide 107 at the bottom of the first material bin 111 to move, so as to place the glass slide 107 on the stage 121. After the glass slide 107 is placed on the stage 121, the push plate 116 is against the stage 121. At this time, the push plate 116 continues to move forward to move the stage 121 a first preset distance, which can be the width of the stage 121, so that after being pushed forward, the next stage 121 can flow to the glass slide 107 placement area to form a continuous and complete action.

[0049] like Figure 3 As shown, the circulating transmission assembly 120 also includes a closed sliding rail 122, and at least one group of propulsion assemblies 129 cooperating with the sliding rail. The propulsion assembly 129 includes a second motor 123, and a second lead screw 124 drivingly connected to the second motor 123. A second slider 125 is provided on the second lead screw 124. The second slider 125 is slidably connected to the third sliding rail 126. A push rod 127 is also provided on the slider. The push rod 127 is used to push the worktable 121 to a second preset distance.

[0050] Specifically, when the stage 121 corresponds to the slide loading assembly 110 and the sedimentation bin placement assembly 130, after completing the loading of the slide 107 and the placement of the sedimentation bin 128, it is pushed forward under the action of the push plate 116. When the stage 121 needs to change the direction of flow on the circulating transmission assembly 120, a pushing assembly 1637 can be set at the corner so that the stage 121 can normally transition its position. For example, when the sliding track of the circulating transmission assembly 120 forms a rectangular closed track, a pushing assembly 129 can be set at the corner, and the slide loading assembly 110 and the sedimentation bin placement assembly 130 can be set at one corner of the rectangular closed track to complete the loading of the slide 107 and the placement of the sedimentation bin 128. The other three corners are all provided with pushing assemblies 129 to form the required circulation.

[0051] When the stage 121 is advanced, considering the influence of the glass slide 107 on the advancement of the stage 121 after the glass slide 107 is loaded, the area that does not affect the normal flow of the stage 121 can be advanced by the first preset distance, so that the next stage 121 can be located at this station. For the area that may affect the advancement of the stage 121, such as the area adjacent to the first preset distance in the rectangular closed track, it can be advanced by the second preset distance, and the second preset distance can be the length of the side adjacent to the first preset distance in the rectangular closed track.

[0052] like Figure 4 As shown, the sedimentation bin placement assembly 130 includes a discharge assembly and a barrel 131 that cooperates with the discharge assembly. The barrel 131 is used to stack the sedimentation bins 128. The bottom of the barrel 131 has a notch to expose the sedimentation bins 128. The discharge assembly includes a first support frame 132 and a third motor 133 arranged on the first support frame 132. The third motor 133 is drivingly connected to the third lead screw 134. The third lead screw 134 is provided with a third slider 135. The third slider 135 is slidably connected to the first support frame 132. The third slider 135 is provided with a first manipulator 136. The first manipulator 136 is used to grab the sedimentation bin 128.

[0053] Specifically, the first manipulator 136 is used to grab the sedimentation bin 128 at the bottom of the barrel 131. Since the sedimentation bins 128 are stacked in the barrel 131, when the sedimentation bin 128 at the bottom is taken out, the other sedimentation bins 128 fall down in sequence to facilitate normal grabbing next time. For example, after the slide 107 is loaded on the stage 121, the third motor 133 drives the first manipulator to approach the sedimentation bin 128, and after grabbing the sedimentation bin 128, the first manipulator 136 is driven to retract to the top of the stage 121, and the sedimentation bin 128 is placed on the slide 107 to form the required matching relationship.

[0054] In an optional embodiment of the present application, magnets are provided on the sedimentation chamber 128 and the stage 121 so that the sedimentation chamber 128 and the stage 121 can be attracted to each other. When the glass slide 107 is clamped between the two, the sedimentation chamber 128 and the glass slide 107 can be in close contact with each other. When the separation liquid or sample liquid is injected into the sedimentation chamber 128, leakage of the liquid can be avoided, which is beneficial to improving the stability of the preparation process.

[0055] like Figure 5 As shown, the sedimentation bin removal assembly 140 includes a bracket 141, and a first motor 142 arranged on the bracket 141, the first motor 142 is transmission-connected to the nut screw 143, a sliding block 144 is arranged on the nut screw 143, a first support plate 145 is arranged on the sliding block 144, a second motor 146 is arranged on the first support plate 145, the second motor 146 is connected to the second support plate 147, a second manipulator 148 is arranged on the second support plate 147, and the second manipulator 148 is used to grab the sedimentation bin 128.

[0056] Specifically, when the sedimentation bin 128 needs to be taken out after the film-making process is completed, the sedimentation bin 128 needs to be grabbed by the second manipulator 148, and the sliding block 144 slides along the extension direction of the nut screw 143 under the action of the first motor 142 to adjust the appropriate height when grabbing. After the sedimentation bin 128 is grabbed by the second manipulator 148, the second motor 146 can be used to rotate a preset angle, which can be 90° or 120°, so that the sedimentation bin 128 is grabbed to the collection box, so as to facilitate the recycling of the sedimentation bin 128 after use.

[0057] like Figure 6 As shown, the glass slide removal component 150 includes a material removal component and a material box 151 that cooperates with the material removal component. A plurality of protrusions are arranged at intervals in the material box 151 so that the glass slides 107 can be placed at intervals in the material box 151; the material removal component includes a second support frame 152, and a first drive motor 153 arranged on the second support frame 152, the first drive motor 153 is drivingly connected to the first screw rod 154, the first screw rod 154 is provided with a first sliding block 155, the first sliding block 155 is slidably connected to the second support frame 152, the first sliding block 155 is provided with a fixed plate 156, the fixed plate 156 is provided with a second drive motor 157, the second drive motor 157 is drivingly connected to the second screw rod, the second screw rod is provided with a second sliding block 159, the second sliding block 159 is slidably connected to the fixed plate 156, the second sliding block 159 is provided with a third manipulator 1592, and the third manipulator 1592 is used to grab the glass slide 107.

[0058] Specifically, when the prepared glass slide 107 is taken out and collected, the first slider 114 is moved up and down by the first driving motor 153, so that the third manipulator 1592 corresponds to the glass slide 107 to grab the glass slide 107. After the glass slide 107 is grabbed, the first driving motor 153 and the second driving motor 157 can be respectively operated to place the gripped glass slide 107 at different placement positions in the material box 151, until the storage space of the material box 151 is full and a new material box 151 is replaced to place the glass slide 107.

[0059] like Figure 7 As shown, the liquid inlet component 160 includes a first mounting plate 161 and a second mounting plate 162 which are spaced apart. The first mounting plate 161 is provided with at least one group of translation components 163. The second mounting plate 162 is used to place a sample rack 164. The translation component 163 is used to push the sample rack 164 to translate on the first mounting plate 161. The sample rack 164 is used to place a sample bottle 165 containing sample liquid. A sampling component 166 is also provided on the first mounting plate 161. A sample injection component 167 which is connected to the sampling component 166 is provided on the operating table 105 for injecting the extracted sample liquid into the sedimentation chamber 128.

[0060] Specifically, the sample rack 164 placed on the second mounting plate 162 can be moved according to the translation assembly 163 disposed on the first mounting plate 161. Figure 7 The sample rack 164 moves in the direction of the arrow. Figure 7 When the sample rack 164 moves in the direction of the right arrow in the figure, it moves to the side close to the sampling assembly 166 and moves to the right by the distance between adjacent sample bottles 165 at a time, so that the sample bottles 165 can correspond to the sampling assembly 166, which facilitates the liquid extraction operation of the sampling assembly 166. When the sample rack 164 moves to the right side of the sampling assembly 166, the sample rack 164 moves to the side close to the sampling assembly 166 and moves to the right by the distance between adjacent sample bottles 165 at a time, so that the sample bottles 165 can correspond to the sampling assembly 166, which facilitates the liquid extraction operation of the sampling assembly 166. Figure 7 Move in the direction of the left arrow in the figure to push out the sample rack 164 to load a new sample bottle 165.

[0061] like Figure 8 As shown, the translation assembly 163 includes a rotating motor 1631, and a synchronous wheel 1632 is provided at the output end of the rotating motor 1631. The synchronous wheel 1632 is connected to the idler wheel 1634 through a synchronous belt 1633; the translation assembly 163 also includes a linear slide rail 1635, and a connecting plate 1636 is slidably provided on the linear slide rail 1635. The connecting plate 1636 is fixedly connected to the synchronous belt 1633, and a pushing assembly 1637 is provided on the connecting plate 1636 for pushing the sample rack 164.

[0062] Specifically, when the sample rack 164 needs to be pushed to move, the synchronous belt 1633 is driven to move by the rotating motor 1631, and the synchronous belt 1633 drives the connecting plate 1636 to move synchronously, and the connecting plate 1636 drives the pushing component 1637 to move, so that the pushing component 1637 pushes the sample rack 164 to move horizontally on the second mounting plate 162. In order to facilitate the cooperation between the pushing component 1637 and the sample rack 164, a strip slot can be provided on the second mounting plate 162, so that the pushing component 1637 passes through the strip slot to push the sample rack 164 to move.

[0063] In an optional embodiment of the present application, the pushing component 1637 can be provided in various forms to meet the needs of different scenarios. Figure 8 As shown, the pushing components 1637 can be arranged at intervals to make the sample rack 164 more stable when being pushed, avoid pushing it tilted, and help improve the stability of the film making process. Fig. 9 As shown, the pushing assembly 1637 can be arranged on the same plate surface, and the pushing stroke is in the same straight line. A strip slot hole can be opened on the second mounting plate 162 to facilitate the short-distance pushing of the sample rack 164. Fig.10 As shown, the pushing component 1637 can be connected by a U-shaped arm, so that the pushing component 1637 can push the sample rack 164 from one side of the second mounting plate 162 to avoid opening a strip slot on the second mounting plate 162.

[0064] like Fig.11 As shown, the sampling component 166 includes a first rotary driver 1661, a first turntable 1662 connected to the first rotary driver 1661, a first bracket 1663 arranged on the first turntable 1662, and a first sampling needle 1664 and a second sampling needle 1665 respectively arranged on both sides of the first bracket 1663. The first sampling needle 1664 corresponds to the sample bottle 165 below, and a cleaning tank 1666 for containing cleaning liquid is arranged below the second sampling needle 1665. The first rotary driver 1661 drives the first turntable 1662 to rotate so that the position of the first sampling needle 1664 and the position of the second sampling needle 1665 are interchanged.

[0065] Specifically, in one state, the position of the first sampling needle 1664 corresponds to being located above the sample bottle 165, so that the specimen liquid in the sample bottle 165 can be extracted through the first sampling needle 1664, and the position of the second sampling needle 1665 corresponds to being located above the cleaning tank 1666, so that the cleaning liquid in the cleaning tank 1666 can be extracted through the second sampling needle 1665 to perform a cleaning operation on the second sampling needle 1665. Subsequently, after the first sampling needle 1664 completes the extraction work, a cleaning operation is required to avoid cross contamination, and the second sampling needle 1665 has been cleaned and can perform a sampling operation. At this time, the first bracket 1663 is driven to rotate by the first rotary driver 1661, so that the positions of the first sampling needle 1664 and the second sampling needle 1665 are interchanged, thereby realizing a cyclic extraction and cleaning operation.

[0066] It can be understood that in order to facilitate the extraction operation of the first sampling needle 1664 and the second sampling needle 1665, the first sampling needle 1664 and the second sampling needle 1665 can be set on the up and down translation component 163 to adjust the height of the first sampling needle 1664 and the second sampling needle 1665, thereby realizing the required operation process.

[0067] like Fig.12 As shown, the injection assembly 167 includes a second rotary driver 1671, a second bracket 1672, and a first injection needle 1673 and a second injection needle 1674 respectively arranged on both sides of the second bracket 1672, the lower side of the first injection needle 1673 corresponds to the sedimentation chamber 128, and a recovery tank for recovering the cleaning liquid is arranged correspondingly below the second injection needle 1674, the tail end of the first injection needle 1673 is connected to the tail end of the first sampling needle 1664 through a pipeline, the tail end of the second injection needle 1674 is connected to the tail end of the second sampling needle 1665 through a pipeline, the driving end of the second rotary driver 1671 is transmission-connected to the second bracket 1672, and the second rotary driver 1671 drives the second bracket 1672 to rotate so that the position of the first injection needle 1673 and the position of the second injection needle 1674 are interchanged.

[0068] Specifically, in one state, a sedimentation chamber 128 ( Fig.12 The position of the sedimentation chamber 128 and the position of the recovery trough are described here with reference to the position of the first injection needle 1673 and the position of the second injection needle 1674 in this state, and it does not mean that the sedimentation chamber 128 must always be located below the first injection needle 1673 and the recovery trough must always be located below the second injection needle 1674.

[0069] Since the tail end of the first injection needle 1673 is connected to the tail end of the first sampling needle 1664 through a pipeline, and the tail end of the second injection needle 1674 is connected to the tail end of the second sampling needle 1665 through a pipeline, in the above state, the position of the first injection needle 1673 corresponds to the upper part of the sedimentation chamber 128, so that the specimen liquid extracted by the first sampling needle 1664 is injected into the sedimentation chamber 128 through the first injection needle 1673, thereby performing the injection operation through the first injection needle 1673, and the position of the second injection needle 1674 corresponds to the upper part of the recovery tank, so that the cleaning liquid extracted by the second sampling needle 1665 is injected into the recovery tank through the second injection needle 1674, thereby performing the cleaning operation on the second injection needle 1674. Subsequently, the first injection needle 1673 is contaminated and needs to be cleaned, while the second injection needle 1674 has been cleaned and can perform the injection operation. At this time, the second support 1672 is driven to rotate by the second rotary driver 1671, so that the positions of the first injection needle 1673 and the second injection needle 1674 are interchanged, thereby realizing the operation of cyclic injection and cleaning.

[0070] It is understandable that in order to facilitate the injection operation of the first injection needle 1673 and the second injection needle 1674, the first injection needle 1673 and the second injection needle 1674 can also be set on the up and down translation component 163 to adjust the height of the first injection needle 1673 and the second injection needle 1674, thereby realizing the required operation process.

[0071] like Figure 1 and Fig.13 As shown, the cell preparation device 100 also includes a coding component 170, which includes an information acquisition component 172, an information processing component 174 and a laser coder 176 that are electrically connected to each other. The information acquisition component 172 is arranged on one side of the sample rack 164, and the laser coder 176 is located above the stage 121; wherein, the information acquisition component 172 is used to transmit the sample information of the collected sample bottle 165 to the information processing component 174, and the information processing component 174 is used to control the laser coder 176 to code the slide 107 according to the sample information.

[0072] Specifically, in the process of collecting the patient's specimen fluid through the specimen bottle 165, in order to distinguish the specimen bottles 165 containing different specimen fluids, the specimen bottle 165 is generally provided with information of the specimen fluid contained in the specimen bottle 165. Therefore, in the process of cell preparation, in order to make a one-to-one correspondence between the specimen bottle 165 and the prepared slide glass 107 obtained by extracting the specimen fluid in the specimen bottle 165, and to avoid the problem of complicated and error-prone information statistics due to too many specimen bottles 165, the information collection component 172, the information processing component 174 and the laser coder 176 of the coding component 170 are used to automatically mark and code the slide glass 107, thereby significantly improving the efficiency and accuracy of preparation.

[0073] like Fig.14 As shown, the liquid extraction component 180 includes a liquid extraction pump 182 , a liquid extraction pipeline 184 and a waste liquid bottle 186 . The liquid extraction pump 182 is disposed on the liquid extraction pipeline 184 and is used to extract the supernatant into the waste liquid bottle 186 through the liquid extraction pipeline 184 .

[0074] Specifically, the liquid pump 182 can be a peristaltic pump to extract the supernatant formed in the sedimentation chamber 128 with high precision to facilitate the subsequent dyeing process. It is understandable that in order to avoid the problem of cross contamination caused by the use of the liquid extraction pipeline 184 to extract the supernatant in different sedimentation chambers 128, a corresponding cleaning component can also be set for the liquid extraction component 180 to clean the liquid extraction pipeline 184 after the liquid extraction is completed.

[0075] like Fig.15 As shown, the liquid adding assembly 190 includes at least one liquid supply bottle 192 , a liquid supply pipeline 194 connected to the liquid supply bottle 192 , and a liquid supply pump 196 is provided on the liquid supply pipeline 194 for pumping the liquid in the liquid supply bottle 192 into the sedimentation bin 128 through the liquid supply pipeline 194 .

[0076] Specifically, the liquid supply bottle 192 may include a separation liquid bottle, a buffer liquid bottle, a dye liquid bottle, a rinse liquid bottle, and a transparent liquid bottle, etc., according to the different liquids in the bottle. Each different liquid supply bottle 192 has one or more liquid supply pipelines 194 connected thereto, and each liquid supply pipeline 194 is provided with a separate liquid supply pump 196 to achieve injection of different liquids at different times. In this way, each liquid has its own independent circulation pipeline, which will not cause mutual contamination between different types of liquids, and is conducive to improving the stability of liquid supply.

[0077] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell preparation device, characterized in that: The invention comprises an operating table, and a glass slide loading assembly, a circulating transmission assembly, a sedimentation bin placing assembly, a sedimentation bin taking out assembly, and a glass slide taking out assembly arranged on the operating table. The operating table is also provided with a liquid inlet assembly, a liquid extraction assembly, and a liquid adding assembly, wherein the circulating transmission assembly comprises a stage, the glass slide loading assembly is used to load a glass slide on the stage, and the sedimentation bin placing assembly is used to place a sedimentation bin on the glass slide; The liquid inlet component injects the sample liquid into the sedimentation chamber, and when the stage flows to different positions, the liquid extraction component extracts the supernatant formed in the sedimentation chamber; the liquid adding component is used to sequentially add separation liquid, buffer solution, staining liquid, washing liquid and transparent liquid into the sedimentation chamber, the sedimentation chamber removal component removes the sedimentation chamber, and the slide removal component removes and collects the slide after preparation; The slide loading assembly includes a transmission assembly and a first silo that cooperates with the transmission assembly, wherein the first silo is used to store the slides; the transmission assembly includes a first motor and a first lead screw that is drive-connected to the first motor, a first slider is provided on the first lead screw, the first slider is slidably connected to a first slide rail, a push plate is further provided on the first slider, the push plate is slidably connected to a second slide rail, the push plate is used to load the slides onto the stage and push the stage to move a first preset distance; the circulating transmission assembly also includes a third slide rail and at least one group of propulsion assemblies that cooperate with the third slide rail, the propulsion assembly includes a second motor, And a second lead screw driven and connected to the second motor, a second slider is provided on the second lead screw, the second slider is slidably connected to the third slide rail, and a push rod is also provided on the second slide rail, and the push rod is used to push the loading platform a second preset distance; the sedimentation bin placement assembly includes a discharge assembly and a barrel matched with the discharge assembly, the barrel is used to stack the sedimentation bins, the bottom of the barrel has a notch to expose the sedimentation bin, the discharge assembly includes a first support frame, and a third motor arranged on the first support frame, the third motor is driven and connected to the third lead screw, the third slider is provided on the third lead screw, and the third slider is slidably connected to the first The support frame is slidably connected, and a first manipulator is arranged on the third sliding block, and the first manipulator is used to grab the sedimentation bin; the sedimentation bin taking out component includes a bracket, and a first motor arranged on the bracket, the first motor is connected to the nut screw in a transmission manner, the nut screw is provided with a sliding block, a first support plate is arranged on the sliding block, a second motor is arranged on the first support plate, the second motor is connected to the second support plate, a second manipulator is arranged on the second support plate, and the second manipulator is used to grab the sedimentation bin; the slide taking out component includes a material taking component, and a material box cooperating with the material taking component, and a plurality of intervals are arranged in the material box. a protrusion so that the glass slides can be placed at intervals in the material box; the material picking assembly includes a second support frame, and a first drive motor arranged on the second support frame, the first drive motor is drivingly connected to the first screw rod, the first screw rod is provided with a first sliding block, the first sliding block is slidably connected to the second support frame, the first sliding block is provided with a fixed plate, the fixed plate is provided with a second drive motor, the second drive motor is drivingly connected to the second screw rod, the second screw rod is provided with a second sliding block, the second sliding block is slidably connected to the fixed plate, the second sliding block is provided with a third manipulator, and the third manipulator is used to grab the glass slide.

2. The cell preparation device according to claim 1, characterized in that: The liquid inlet assembly includes a first mounting plate and a second mounting plate that are spaced apart, at least one set of translation assemblies is arranged on the first mounting plate, the second mounting plate is used to place a sample rack, the translation assembly is used to push the sample rack to translate on the first mounting plate, the sample rack is used to place a sample bottle containing sample liquid, a liquid extraction assembly is also arranged on the first mounting plate, and a sample injection assembly connected to the liquid extraction assembly is arranged on the operating table, which is used to inject the extracted sample liquid into the sedimentation chamber.

3. The cell preparation device according to claim 2, characterized in that: The translation assembly includes a rotating motor, a synchronous wheel is provided at the output end of the rotating motor, and the synchronous wheel is connected to the idle wheel through a synchronous belt; the translation assembly also includes a linear slide rail, a connecting plate is slidably provided on the linear slide rail, the connecting plate is fixedly connected to the synchronous belt, and a pushing assembly is provided on the connecting plate for pushing the sample rack.

4. The cell preparation device according to claim 2, characterized in that: The liquid extraction assembly includes a first rotary driver, a first turntable connected to the first rotary driver, a first bracket arranged on the first turntable, and a first sampling needle and a second sampling needle respectively arranged on both sides of the first bracket, the first sampling needle corresponds to the sample bottle below, and the second sampling needle corresponds to a cleaning tank for containing cleaning liquid below, and the first rotary driver drives the first turntable to rotate so that the position of the first sampling needle and the position of the second sampling needle are interchanged; The sampling assembly includes a second rotating driver, a second bracket, and a first sampling needle and a second sampling needle respectively arranged on both sides of the second bracket, the lower side of the first sampling needle corresponds to the sedimentation bin, and a recovery groove for recovering the cleaning liquid is arranged correspondingly below the second sampling needle. The tail end of the first sampling needle is connected to the tail end of the first sampling needle through a pipeline, and the tail end of the second sampling needle is connected to the tail end of the second sampling needle through a pipeline. The driving end of the second rotating driver is transmission-connected to the second bracket, and the second rotating driver drives the second bracket to rotate so that the position of the first sampling needle and the position of the second sampling needle are interchanged.

5. The cell preparation device according to claim 2, characterized in that: The cell preparation device also includes a coding component, which includes an information acquisition component, an information processing component and a laser coder that are electrically connected to each other, the information acquisition component is arranged on one side of the sample rack, and the laser coder is located above the stage; wherein the information acquisition component is used to transmit the sample information of the collected sample bottle to the information processing component, and the information processing component is used to control the laser coder to code the glass slide according to the sample information.

6. The cell preparation device according to claim 1, characterized in that: The liquid extraction component comprises a liquid extraction pump, a liquid extraction pipeline and a waste liquid bottle. The liquid extraction pump is arranged on the liquid extraction pipeline and is used to extract the supernatant into the waste liquid bottle through the liquid extraction pipeline.

7. The cell preparation device according to claim 1, characterized in that: The liquid adding assembly includes at least one liquid supply bottle and a liquid supply pipeline connected to the liquid supply bottle. The liquid supply pipeline is provided with a liquid supply pump for pumping the liquid in the liquid supply bottle into the sedimentation bin through the liquid supply pipeline.

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