Application of novel zinc phthalocyanine photosensitizer combined with photodynamic therapy in tooth bleaching

Abstract

The invention discloses application of a novel zinc phthalocyanine photosensitizer combined with photodynamic therapy in tooth bleaching. The application comprises the following steps of S1, preparing a penta-polylysine beta-carbonyl zinc phthalocyanine solution; S2, performing grouping; S3, respectively dripping on six sample tooth blocks in the corresponding groups; S4, vertically irradiating the six groups of sample tooth blocks for 30 minutes at a position about 20cm away from the sample tooth blocks by using an LED lamp with the wavelength of 680nm; S5, performing an experiment for two periods; S6, after each time of bleaching is finished, removing bleaching substances on the surface of each sample tooth block, and washing with deionized water; S7, measuring data; and S8, obtaining an experiment result. The invention provides the application of the novel zinc phthalocyanine photosensitizer combined with photodynamic therapy in tooth bleaching, and solves the problems of tooth sensitivity, soft tissue stimulation, reduction of enamel microhardness, increase of tooth roughness and other bleaching side effects in the prior art of tooth bleaching.

Description

technical field

[0001] The invention relates to the technical field of tooth bleaching and photodynamic therapy, in particular to the application of a novel zinc phthalocyanine photosensitizer combined with photodynamic therapy in tooth bleaching. Background technique

[0002] With the rapid development of social economy, people's requirements for beauty are constantly improving, and the demand for tooth whitening is also increasing. Commonly used tooth whitening methods include tooth bleaching, resin restoration, all-ceramic crown and veneer restoration. These methods have their own advantages and disadvantages, among which tooth bleaching is widely welcomed by dental professionals and patients as an effective and relatively conservative treatment. Tooth bleaching refers to a cosmetic method that changes the internal and external coloring of teeth through the action of bleaching agents. The active oxygen generated by the chemical decomposition of the bleach decomposes the...

Examples

Embodiment 1

[0039] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: after dissolving pentalysine β-carbonyl phthalocyanine zinc powder with DMSO, measure the concentration of the obtained solution by microplate reader, dilute with deionized water to the required concentration.

Embodiment 2

[0041] In Vitro Experimental Study on Pentalysine β-Carbonyl Phthalocyanine Zinc (Preliminary Experiment)

[0042] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: dissolve pentalysine β-carbonyl phthalocyanine zinc solid with DMSO solution, measure the concentration of the obtained solution with a microplate reader and dilute with deionized water to the required Concentration, the specific preparation method in the present embodiment is: after dissolving the 0.005-0.01g pentapolylysine β-carbonyl phthalocyanine zinc solid with 5-10ml of DMSO solution, measure the concentration of the resulting solution by a microplate reader, this The concentration determined in the experiment is 433μM, which is used as the mother solution prepared from solutions with different concentrations.

[0043] Prepare 5 μM pentalysine β-carbonyl zinc phthalocyanine solution: take 35 μL of the mother solution, add 2996 μL of deionized water, and mix thoroughly to obtain a total of 3...

Embodiment 3

[0058] In Vitro Experimental Study on Pentalysine β-Carbonyl Phthalocyanine Zinc II

[0059] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: based on the experimental results of Example 2, pentalysine β-carbonyl phthalocyanine zinc with a concentration of 20 μM is selected as the research solution in this example, and its preparation method is the same as Pentalysine β-carbonyl phthalocyanine zinc preparation method of 20 μM concentration in embodiment two;

[0060] Grouping: A total of 90 tooth block samples were randomly divided into three groups, with 30 tooth block samples in each group, of which 15 samples were used for color and surface roughness measurement before and after bleaching, and the enamel surface was not specially treated, and the remaining 15 The enamel surface of the sample tooth block was polished with silicon carbide paper until the surface was completely flat, which was used to measure the microhardness;

[0061] Group 1 (experimenta...

technical field

[0001] The invention relates to the technical field of tooth bleaching and photodynamic therapy, in particular to the application of a novel zinc phthalocyanine photosensitizer combined with photodynamic therapy in tooth bleaching. Background technique

[0002] With the rapid development of social economy, people's requirements for beauty are constantly improving, and the demand for tooth whitening is also increasing. Commonly used tooth whitening methods include tooth bleaching, resin restoration, all-ceramic crown and veneer restoration. These methods have their own advantages and disadvantages, among which tooth bleaching is widely welcomed by dental professionals and patients as an effective and relatively conservative treatment. Tooth bleaching refers to a cosmetic method that changes the internal and external coloring of teeth through the action of bleaching agents. The active oxygen generated by the chemical decomposition of the bleach decomposes the...

Examples

Embodiment 1

[0039] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: after dissolving pentalysine β-carbonyl phthalocyanine zinc powder with DMSO, measure the concentration of the obtained solution by microplate reader, dilute with deionized water to the required concentration.

Embodiment 2

[0041] In Vitro Experimental Study on Pentalysine β-Carbonyl Phthalocyanine Zinc (Preliminary Experiment)

[0042] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: dissolve pentalysine β-carbonyl phthalocyanine zinc solid with DMSO solution, measure the concentration of the obtained solution with a microplate reader and dilute with deionized water to the required Concentration, the specific preparation method in the present embodiment is: after dissolving the 0.005-0.01g pentapolylysine β-carbonyl phthalocyanine zinc solid with 5-10ml of DMSO solution, measure the concentration of the resulting solution by a microplate reader, this The concentration determined in the experiment is 433μM, which is used as the mother solution prepared from solutions with different concentrations.

[0043] Prepare 5 μM pentalysine β-carbonyl zinc phthalocyanine solution: take 35 μL of the mother solution, add 2996 μL of deionized water, and mix thoroughly to obtain a total of 3...

Embodiment 3

[0058] In Vitro Experimental Study on Pentalysine β-Carbonyl Phthalocyanine Zinc II

[0059] Preparation of pentalysine β-carbonyl phthalocyanine zinc solution: based on the experimental results of Example 2, pentalysine β-carbonyl phthalocyanine zinc with a concentration of 20 μM is selected as the research solution in this example, and its preparation method is the same as Pentalysine β-carbonyl phthalocyanine zinc preparation method of 20 μM concentration in embodiment two;

[0060] Grouping: A total of 90 tooth block samples were randomly divided into three groups, with 30 tooth block samples in each group, of which 15 samples were used for color and surface roughness measurement before and after bleaching, and the enamel surface was not specially treated, and the remaining 15 The enamel surface of the sample tooth block was polished with silicon carbide paper until the surface was completely flat, which was used to measure the microhardness;

[0061] Group 1 (experimenta...

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