microfluidic chip

Abstract

The present invention relates to a microfluidic chip having a reagent storage structure, wherein the reagent storage structure includes a reagent addition groove, a feed penetration hole, a feed microchannel, a first feed microfluidic valve and a material storage cavity. When pre-storing reagents, the reagents are added to the reagent addition groove, and the reagents enter the feed microchannel through the feed penetration hole. Through rotation and centrifugation, the reagents break through the first feed microfluidic valve and enter the material storage cavity. The first feed microfluidic valve can prevent the reagents from flowing back from the material storage cavity, thereby realizing the storage of the reagents. The above-mentioned microfluidic chip encapsulates the reagents required for detection into the microfluidic chip through structural design. It can be understood that the material storage cavity can be connected to the detection liquid path. When the detection is required, the reagent is driven into the detection liquid path by increasing the centrifugal force. The above-mentioned microfluidic chip realizes the microfluidic project detection of the integrated reagent chip, which can save the operation of adding corresponding reagents in the test link, is easy to use, and saves time.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, in particular to a microfluidic chip. Background Art

[0002] Chemiluminescent immunoassay refers to the generation of photons by chemical reactions without external light sources, heat or electric fields. It can combine highly sensitive chemiluminescent detection technology with highly specific antigen-antibody immune reactions to detect the antigen or antibody content in the sample.

[0003] Point-of-care testing (POCT) refers to clinical and bedside testing performed next to the patient. It is usually analyzed immediately at the sampling site, eliminating the complex processing procedures of the specimen in the laboratory and providing a method to quickly obtain test results.

[0004] Microfluidic chips are a hot area of ​​development in miniaturized total analysis systems, and can be applied to point-of-care chemiluminescent immunoassays. Microfluidic chips integrate a range of experimental procedures across a wide range of biological, chemical, and medical fields, including sample pretreatment, sample reaction, and result reading, onto a single micro- and nanoscale chip. They offer advantages such as minimal sample and reagent consumption, rapid reaction times, and accurate results. Centrifugal microfluidic chips are a type of microfluidic chip that utilize centrifugal force to propel samples or reagents through microchannels for detection. Typically, multiple basic units capable of performing a single reaction are integrated onto a single chip, enabling multi-sample testing. Adjusting the centrifugal speed to achieve varying centrifugal forces controls the movement of liquids on the chip. Centrifugal microfluidic chips offer advantages such as highly symmetrical structure, large sample sizes, minimal external power requirements, minimal supporting infrastructure, high automation, accurate measurement structures, and excellent reproducibility.

[0005] Most existing centrifugal microfluidic chips use a design that separates reagents from the test disc, which increases the pipeline design of the detection instrument and the number of reagent addition steps. This leads to problems such as long experimental time, unstable reagent addition volume, and reagent waste. As a result, the reaction rate, reaction stability, and cost of the microfluidic chip cannot meet the needs of in vitro diagnosis, especially point-of-care (POCT) diagnosis, which puts the practicality of the centrifugal microfluidic chip under scrutiny. Summary of the Invention

[0006] Based on this, it is necessary to provide a microfluidic chip that can pre-store materials.

[0007] The scheme of the present invention is as follows:

[0008] A microfluidic chip has a reagent storage structure, which includes a reagent addition groove, a feed penetration hole, a feed microchannel, a first feed microfluidic valve and a material storage cavity; the reagent addition groove opens to a side surface of the microfluidic chip, the reagent addition groove is connected to the feed microchannel through the feed penetration hole, and the feed microchannel is connected to the material storage cavity through the first feed microfluidic valve; the microfluidic chip has a rotation center, and the material storage cavity is further away from the rotation center than the reagent addition port.

[0009] In one embodiment, one end of the feed permeation hole opens to the bottom of the reagent addition tank to communicate with the reagent addition tank.

[0010] In one embodiment, the extending direction of the feed permeation hole is perpendicular to the disk surface of the microfluidic chip.

[0011] In one embodiment, the first feed microfluidic valve includes a first section, a second section, and a third section that are connected in sequence, one end of the first section is connected to the feed microfluidic channel, one end of the third section is connected to the material storage chamber, the first section and the third section extend away from the rotation center, the third section is farther away from the rotation center than the first section, and the first section and the third section are respectively arranged at an angle to the second section.

[0012] In one embodiment, one end of the first feed micro-flow valve that is in communication with the material storage chamber opens to a side wall of the material storage chamber.

[0013] In one embodiment, the first feed micro-flow valve is a hydrophobic valve or a capillary valve.

[0014] In one embodiment, the reagent storage structure further includes a second feed microfluidic valve, and the feed permeation hole is connected to the feed microfluidic channel through the second feed microfluidic valve.

[0015] In one embodiment, the microfluidic chip further includes a solvent addition hole in zone C, the reagent storage structure further includes a third microchannel, the third microchannel is connected to the material storage cavity, the solvent addition hole in zone C is connected to the third microchannel, and the material storage cavity is farther away from the rotation center than the solvent addition hole in zone C.

[0016] In one embodiment, there are multiple reagent storage structures, and the multiple reagent storage structures are distributed around the rotation center and arranged at intervals.

[0017] In one embodiment, the microfluidic chip further includes a first microchannel in zone C and a distribution chamber in zone C. The distribution chamber in zone C is arranged around the rotation center. The distribution chamber in zone C is connected to the solvent addition hole in zone C through the first microchannel in zone C. The distribution chamber in zone C is connected to the material storage chamber through the third microchannel. The plurality of reagent storage structures are distributed along the extension direction of the distribution chamber in zone C. The distances between the solvent addition hole in zone C, the distribution chamber in zone C and the material storage chamber and the rotation center increase successively.

[0018] In one embodiment, the zone C distribution chamber includes a plurality of sub-distribution chambers, which are distributed around the rotation center and spaced apart, and each of the sub-distribution chambers is connected to a plurality of the reagent storage structures.

[0019] In one embodiment, the microfluidic chip includes a sample solvent addition layer and a reaction layer arranged in a stacked manner;

[0020] The sample solvent addition layer comprises a sample addition hole in area C, a solvent addition hole in area C, a first microfluidic channel in area C, a distribution cavity in area C, and the reagent storage structure. The reagent storage structure further comprises a fourth microfluidic channel in area C and a second connection port in area C. The material storage cavity is connected to the second connection port in area C via the fourth microfluidic channel in area C.

[0021] The reaction layer has multiple distribution reaction structures, which are distributed and spaced around the rotation center; the distribution reaction structure includes a sample addition hole in area A, a distribution chamber in area A, and a reaction unit; the sample addition hole in area A is connected to the distribution chamber in area A, and the reaction unit includes a first microchannel in area A and area C and a reaction chamber, and the reaction chamber is connected to the distribution chamber in area A through the first microchannel in area A and area C; the distribution chamber in area A extends around the rotation center, and there are multiple reaction units in the distribution reaction structure, and the multiple reaction units are distributed along the extension direction of the distribution chamber in area A. The distribution chamber in area A is closer to the rotation center than the reaction chamber, the sample addition hole in area A is connected to the sample addition hole in area C, and the reaction chamber is connected to the second connection port in area C.

[0022] In one embodiment, the distribution reaction structure further includes a separation chamber and a second microchannel in zone A. The separation chamber is connected to the zone A distribution chamber through the second microchannel in zone A. The separation chamber is closer to the rotation center than the zone A distribution chamber.

[0023] In one embodiment, the distribution reaction structure further includes a fourth microchannel in zone A and a waste liquid storage chamber. The reaction chamber is connected to the waste liquid storage chamber through the fourth microchannel in zone A. The waste liquid storage chamber is further away from the rotation center than the reaction chamber.

[0024] In one embodiment, magnetic beads labeled with immune components are stored in the reaction chamber, and the immune components are antigens or antibodies.

[0025] Compared with existing solutions, the above microfluidic chip has the following beneficial effects:

[0026] The above-mentioned microfluidic chip has a reagent storage function and can pre-store reagents before the detection test. When pre-storing reagents, the reagents that need to be pre-stored are added to the reagent addition tank, and the reagents enter the feed microchannel through the feed penetration hole. Through rotation and centrifugation, the reagents break through the first feed microfluidic valve and enter the material storage cavity. The first feed microfluidic valve can prevent the reagent from flowing back from the material storage cavity, thereby realizing the storage of the reagent. The above-mentioned microfluidic chip encapsulates the reagents required for detection into the microfluidic chip through structural design. It can be understood that the material storage cavity can be connected to the detection liquid path. When the detection is required, the reagent is driven into the detection liquid path by increasing the centrifugal force. The above-mentioned microfluidic chip realizes the microfluidic project detection of the integrated reagent chip, which can save the operation of adding corresponding reagents in the test link, is easy to use, and saves time. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Schematic diagram of the structure of a microfluidic chip according to an embodiment of the present invention;

[0028] Figure 2 This is a schematic structural diagram of a reagent storage structure of a microfluidic chip according to an embodiment of the present invention;

[0029] Figure 3 for Figure 2 A cross-sectional view of the reagent storage structure shown;

[0030] Figure 4 To include Figure 2 A schematic structural diagram of a storage unit of the reagent storage structure shown;

[0031] Figure 5 for Figure 1 Schematic diagram of the structure of the sample solvent addition layer in the microfluidic chip shown;

[0032] Figure 6 for Figure 1 Schematic diagram of the positional relationship among the sample solvent addition layer, the common reagent layer, and the sample solvent addition layer in the microfluidic chip shown;

[0033] Figure 7 for Figure 1Schematic diagram of the structure of the sample solvent addition layer of the microfluidic chip shown;

[0034] Figure 8 for Figure 1 Schematic diagram of the structure of the distribution reaction structure of the sample solvent addition layer in the microfluidic chip shown;

[0035] Figure 9 for Figure 1 Schematic diagram of the structure of the common reagent layer in the microfluidic chip shown;

[0036] Figure 10 for Figure 9 A partial enlarged view of

[0037] Figure 11 A diagram showing the connection relationship between a common reagent storage chamber and a reagent output channel without a delay unit;

[0038] Figure 12 A diagram showing the connection relationship between a common reagent storage chamber and a reagent output channel having a delay unit;

[0039] Figure 13 It is a structural diagram of the delay unit;

[0040] Figure 14 This is a diagram showing the connection between a common reagent storage chamber and a reagent output channel having two delay units.

[0041] Description of reference numerals:

[0042] 10. Microfluidic chip; 11. Rotation center; 100. Sample solvent addition layer; 101. Solvent addition port in area C; 103. Sample addition port in area C; 104. First connection port in area C; 105. First microfluidic valve in area C; 106. Second microfluidic valve in area C; 107. Distribution chamber in area C; 108. Third microfluidic valve in area C; 109. Material storage chamber; 110. Fourth microfluidic valve in area C; 111. Second connection port in area C; 112. Third ventilation port Hole; 113, reagent addition slot; 114, feed permeation hole; 115, feed microfluidic channel; 116, first feed microfluidic valve; 1161, first section; 1162, second section; 1163, third section; 117, second feed microfluidic valve; 121, first microfluidic channel in area C; 122, second microfluidic channel in area C; 123, third microfluidic channel in area C; 124, fourth microfluidic channel in area C; 125, fifth microfluidic channel in area C; 130, storage unit;

[0043] 200, common reagent layer; 201, solvent addition hole in area B; 202, common reagent storage chamber; 2021, first sub-storage chamber; 2022, second sub-storage chamber; 2023, third sub-storage chamber; 203, connection port; 204, sample addition hole in area B; 207, distribution chamber in area B; 208, first microfluidic valve in area B; 209, first connection port in area B; 210, second connection port in area B; 211, second connection port in area B Second microfluidic channel; 212, third connection port of area B; 230, diversion unit; 221, first microfluidic channel of area B; 260, reagent output channel; 261, first centrifugal flow channel; 262, first discharge microfluidic valve; 263, delay unit; 2631, first turning flow channel; 2632, capillary flow channel; 2633, second turning flow channel; 2634, second centrifugal flow channel; 2635, second discharge microfluidic valve;

[0044] 300, reaction layer; 330, distribution reaction structure; 301, sample addition hole in area A; 302, separation chamber; 3021, first side; 3022, second side; 3023, third side; 304, distribution chamber in area A; 332, reaction unit; 305, first microfluidic valve in area A; 306, reaction chamber; 307, second reagent inlet in area A; 308, first reagent inlet in area A; 309, second microfluidic valve in area A; 310, waste liquid storage chamber; 312, backflow prevention Flow valve; 321, first microfluidic channel in area A; 322, second microfluidic channel in area A; 3221, first branch channel; 3222, second branch channel; 3223, third branch channel; 323, third microfluidic channel in area A; 324, fourth microfluidic channel in area A; 325, fifth microfluidic channel in area A; 326, sixth microfluidic channel in area A; 327, seventh microfluidic channel in area A; 328, eighth microfluidic channel in area A; 329, ninth microfluidic channel in area A; 401, first vent; 402, second vent. DETAILED DESCRIPTION

[0045] To facilitate understanding of the present invention, the present invention will be described more fully below with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the accompanying drawings. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present disclosure.

[0046] It should be noted that when an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0047] In the description of the present invention, it should be understood that the terms "region A", "region B", "region C", "first", "second", "third", etc. are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number or order of the indicated technical features.

[0048] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0049] like Figure 1 As shown, the present invention provides a microfluidic chip 10. Microfluidic chip 10 has a rotation center 11, on which a specially designed cavity structure 12 is formed. During rotational centrifugation, microfluidic chip 10 rotates about rotation center 11. Fluids can flow through cavity structure 12 under the influence of centrifugal force or capillary force, achieving experimental detection purposes.

[0050] Please refer to Figures 2 to 3 As shown, a microfluidic chip according to one embodiment of the present invention has a reagent storage structure. The reagent storage structure includes a reagent addition slot 113, a feed permeation hole 114, a feed microchannel 115, a first feed microfluidic valve 116, and a material storage chamber 109. The reagent addition slot 113 opens to a side surface of the microfluidic chip 10. The reagent addition slot 113 is connected to the feed microchannel 115 through the feed permeation hole 114. The feed microchannel 115 is connected to the material storage chamber 109 through the first feed microfluidic valve 116. The material storage chamber 109 is further away from the rotation center 11 than the reagent addition port.

[0051] The above-mentioned microfluidic chip 10 has a reagent storage function and can pre-store reagents before the detection test. When pre-storing reagents, the reagents that need to be pre-stored are added to the reagent addition groove 113, and the reagents enter the feed microchannel 115 through the feed penetration hole 114. Through rotation and centrifugation, the reagents break through the first feed microfluidic valve 116 and enter the material storage cavity 109. The first feed microfluidic valve 116 can prevent the reagent from flowing back from the material storage cavity 109, thereby realizing the storage of the reagents. The above-mentioned microfluidic chip 10 encapsulates the reagents required for detection into the microfluidic chip 10 through structural design. It can be understood that the material storage cavity 109 can be connected to the detection liquid path. When the detection is required, the reagent is driven into the detection liquid path by increasing the centrifugal force. The above-mentioned microfluidic chip 10 realizes the microfluidic project detection of the integrated reagent chip, which can save the operation of adding corresponding reagents in the test link, is easy to use, and saves time.

[0052] In one example, one end of the feed penetration hole 114 communicating with the reagent addition tank 113 opens at the bottom of the reagent addition tank 113 , which facilitates the reagent to better enter the feed penetration hole 114 .

[0053] In one example, the extending direction of the feed infiltration hole 114 is perpendicular to the disk surface of the microfluidic chip 10 , which is conducive to better entry of the reagent into the feed microchannel 115 .

[0054] In one example, one end of the first feeding microfluidic valve 116 communicating with the material storage chamber 109 is opened to the side wall of the material storage chamber 109. In this way, the reagent can be better introduced into the material storage chamber 109 by rotating the centrifuge.

[0055] In one example, the first feed micro-flow valve 116 is a hydrophobic valve or a capillary valve.

[0056] like Figure 2 and Figure 3 As shown, in one example, the reagent storage structure further includes a second feed microfluidic valve 117 , and the feed permeation hole 114 is connected to the feed microfluidic channel 115 through the second feed microfluidic valve 117 .

[0057] In the above example, a second feed microfluidic valve 117 is set between the feed penetration hole 114 and the feed microfluidic channel 115. When the reagent is added, the reagent is temporarily retained in the reagent addition tank 113. During centrifugal rotation, the reagent simultaneously enters the feed microfluidic channel 115, thereby reducing the difference. On the other hand, it can prevent backflow during subsequent drying, which is equivalent to an extra layer of insurance.

[0058] like Figure 2 As shown, in one example, the first feed microfluidic valve 116 includes a first section 1161, a second section 1162, and a third section 1163, which are connected in sequence. One end of the first section 1161 is connected to the feed microfluidic channel 115, and one end of the third section 1163 is connected to the material storage chamber 109. The first section 1161 and the third section 1163 extend away from the rotation center 11, with the third section 1163 being further away from the rotation center 11 than the first section 1161. The first section 1161 and the third section 1163 are each arranged at an angle to the second section 1162. For example, the first section 1161 and the third section 1163 are each perpendicular to the second section 1162. This, on the one hand, causes the extension direction of the feed microfluidic channel 115 to deviate from the material storage chamber 109, staggered from other fluid paths connected to the material storage chamber 109, and on the other hand, it can better prevent reagent backflow from the material storage chamber 109.

[0059] like Figure 3As shown, in one example, the sample solvent adding layer 100 includes a bottom plate 151 and a cover plate 152 . The cover plate 152 is provided with a groove structure. The bottom plate 151 and the cover plate 152 are butted together so that the groove structure forms a cavity structure.

[0060] In one example, the material storage chamber 109 stores a second reagent. The second reagent may be, but is not limited to, a chemically labeled antibody, such as an acridine-labeled antibody. The second reagent may be a fluid or a freeze-dried product. The freeze-dried product may be obtained by storing the fluid second reagent in the material storage chamber 109 and then freeze-drying it.

[0061] Traditional chips often require external reagents, resulting in bulky supporting instruments, making it difficult to meet the needs of multi-sample and multi-project testing, and also unable to achieve the results of orderly release of reagents integrated into the chip. In addition, the microfluidic chip 10 described above does not require the addition of common reagents during experiments. The reagents can be automatically and orderly released on the chip, thereby reducing the number of supporting instruments and facilitating the detection of multiple samples and multi-projects.

[0062] The sample solvent addition layer 100 includes a Zone C solvent addition port 101, a Zone C sample addition port 103, a Zone C first microfluidic channel 121, a Zone C distribution chamber 107, a Zone C second microfluidic channel 122, a Zone C first connection port 104, and a reagent storage structure 130. The Zone C distribution chamber 107 is disposed around the rotation center 11. The Zone C solvent addition port 101 communicates with the Zone C distribution chamber 107 via the Zone C first microfluidic channel 121. The Zone C solvent addition port 101 communicates with the Zone C first connection port 104 via the Zone C second microfluidic channel 122.

[0063] like Figure 4 As shown, in one example, the reagent storage structure 130 further includes a third microfluidic channel 123 in area C, a material storage chamber 109, a fourth microfluidic channel 124 in area C, and a second connection port 111 in area C. The material storage chamber 109 communicates with the area C distribution chamber 107 via the third microchannel. The material storage chamber 109 communicates with the second connection port 111 in area C via the fourth microfluidic channel 124 in area C. Multiple reagent storage structures 130 are provided, distributed along the extension direction of the area C distribution chamber 107.

[0064] The distances between the zone C solvent addition hole 101, the zone C distribution chamber 107, the material storage chamber 109 and the zone C second connection port 111 and the rotation center 11 increase in sequence. The zone C first connection port 104 is further away from the rotation center 11 than the zone C solvent addition hole 101.

[0065] In one example, the first micro-fluidic valve 105 of zone C is provided on the first micro-fluidic channel 121 of zone C.

[0066] In one example, the second micro-fluidic valve 106 of zone C is disposed on the second micro-fluidic channel 122 of zone C.

[0067] In one example, a third microfluidic valve 108 of zone C is provided on the third microfluidic channel 123 of zone C.

[0068] In one example, the fourth microfluidic valve 110 of zone C is disposed on the fourth microfluidic channel 124 of zone C.

[0069] In one example, the reagent storage structure 130 further includes a fifth microfluidic channel 125 in area C and a third vent 112. One end of the fifth microfluidic channel 125 in area C is connected to the third microfluidic channel 123 in area C, and the other end is connected to the third vent 112. The third vent 112 opens on a side surface of the microfluidic chip 10.

[0070] like Figure 11 As shown, in one example, the zone C distribution chamber 107 includes multiple sub-distribution chambers, which are distributed and spaced apart around the rotation center 11. Each sub-distribution chamber is connected to multiple reagent storage structures 130. In the specific example shown, the zone C distribution chamber 107 is divided into three sub-distribution chambers, each of which is connected to five reagent storage structures 130.

[0071] Please refer to Figures 5 to 7 As shown, a microfluidic chip 10 according to an embodiment of the present invention includes a sample solvent addition layer 100 and a reaction layer 300 that are stacked.

[0072] The sample solvent addition layer 100 includes a Zone C solvent addition hole 101, a Zone C sample addition hole 103, a Zone C first microfluidic channel 121, a Zone C distribution chamber 107, a Zone C second microfluidic channel 122, a Zone C first connection port 104, and a reagent storage structure 130. The Zone C distribution chamber 107 is disposed around the rotation center 11. The Zone C solvent addition hole 101 communicates with the Zone C distribution chamber 107 via the Zone C first microfluidic channel 121. The Zone C solvent addition hole 101 communicates with the Zone C first connection port 104 via the Zone C second microfluidic channel 122. The reagent storage structure 130 also includes a Zone C fourth microfluidic channel and a Zone C second connection port. The material storage chamber communicates with the Zone C second connection port via the Zone C fourth microfluidic channel.

[0073] like Figure 7 and Figure 8 As shown, the reaction layer 300 has a plurality of distribution reaction structures 330. The plurality of distribution reaction structures 330 are distributed around the rotation center 11 and are spaced apart.

[0074] The distribution reaction structure 330 includes a region A sample addition hole 301, a separation chamber 302, a region A second microfluidic channel 322, a region A distribution chamber 304, and a reaction unit 332. The region A sample addition hole 301 communicates with the separation chamber 302. More specifically, the region A sample addition hole 301 communicates with the separation chamber 302 via the region A third microfluidic channel 323. The separation chamber 302 communicates with the region A distribution chamber 304 via the region A second microfluidic channel 322. The reaction unit 332 includes a region A first microfluidic channel 321 and a reaction chamber 306. The reaction chamber 306 communicates with the region A distribution chamber 304 via the region A first microfluidic channel 321.

[0075] In each distribution reaction structure 330, the zone A distribution chamber 304 extends around the rotation center 11. For example, the zone A distribution chamber 304 may be an arc-shaped chamber centered on the rotation center 11. In each distribution reaction structure 330, there are multiple reaction units 332, distributed along the extension direction of the zone A distribution chamber 304. The separation chamber 302, zone A distribution chamber 304, and reaction chamber 306 are positioned at increasing distances from the rotation center 11.

[0076] The microfluidic chip 10 has a plurality of distribution reaction structures 330, which are arranged around the rotation center 11 and spaced apart. Each distribution reaction structure 330 includes a region A sample addition hole 301, a region A distribution chamber 304, and a reaction unit 332, which are sequentially connected. The reaction unit 332 includes a region A first microfluidic channel 321 and a reaction chamber 306. The reaction chamber 306 is connected to the region A distribution chamber 304 via the region A first microfluidic channel 321. When using the microfluidic chip 10 for testing, a blood sample can be added to the region A sample addition hole 301. The blood sample enters the region A distribution chamber 304 and then enters the region A distribution chamber 304 through the region A first microfluidic channel 321 into the multiple reaction chambers 306 in the corresponding distribution reaction structure 330, where it mixes and reacts with the stored substances. Multiple distribution reaction structures 330 can be used to detect multiple samples. In each distribution reaction structure 330, there are multiple reaction units 332, and multiple reaction units 332 can be used to detect multiple items. In this way, multiple items can be detected on multiple samples at the same time on the same chip, realizing multi-sample multi-item joint testing.

[0077] The number of the distribution reaction structures 330 is at least two, for example, 3 to 10. In the specific example shown in the figure, the microfluidic chip 10 has three distribution reaction structures 330.

[0078] In each distribution reaction structure 330, the number of reaction units 332 is at least two, and can be, for example, 3 to 15. It is understood that the number of reaction units 332 in different distribution reaction structures 330 can be the same or different. In the specific example shown, the number of reaction units 332 in each distribution reaction structure 330 is 5.

[0079] In one example, the distribution reaction structure 330 further includes a separation chamber 302 and a second microfluidic channel 322 in region A. The separation chamber 302 communicates with the region A distribution chamber 304 via the second microfluidic channel 322. The separation chamber 302 is closer to the rotation center 11 than the region A distribution chamber 304. When a whole blood sample passes through the separation chamber 302, plasma is separated and enters the region A distribution chamber 304.

[0080] In one example, the distribution reaction structure 330 further includes a fourth microfluidic channel 324 in zone A and a waste liquid storage chamber 310. The reaction chamber 306 is connected to the waste liquid storage chamber 310 through the fourth microfluidic channel 324 in zone A. The waste liquid storage chamber 310 is further away from the rotation center 11 than the reaction chamber 306.

[0081] In one example, the fourth micro-channel 324 of zone A is provided with a second micro-flow valve 309 of zone A. The second micro-flow valve 309 of zone A can be a steam trap, a capillary valve, or the like.

[0082] In one example, the microfluidic chip 10 further includes a first vent 401 . One end of the first vent 401 is connected to the waste liquid storage chamber 310 , and the other end is open to a side surface of the microfluidic chip 10 .

[0083] In one example, a first microfluidic valve 305 of zone A is provided on the first microfluidic channel 321 of zone A. The first microfluidic valve 305 of zone A can be a steam trap, a capillary valve, or the like.

[0084] In one example, the second microfluidic channel 322 in area A is a U-shaped microfluidic channel, and the opening of the second microfluidic channel 322 in area A faces away from the rotation center 11. More specifically, the second microfluidic channel 322 in area A includes a first branch channel 3221, a second branch channel 3222, and a third branch channel 3223, which are connected in sequence. The first branch channel 3221 is led out from the separation chamber 302, extends close to the rotation center 11, and is connected to one end of the second branch channel 3222. The other end of the second branch channel 3222 is connected to the third branch channel 3223. The third branch channel 3223 is led out from the second branch channel 3222, extends away from the rotation center 11, and is connected to the distribution chamber 304 in area A. More specifically, the first branch channel 3221 is connected to a side surface of the separation chamber 302, which is a surface connecting the side surface of the separation chamber 302 close to the rotation center 11 and the side surface away from the rotation center 11. For example, in the specific example shown in the figure, the separation chamber 302 is a fan-shaped annular cavity extending along the rotational circumference of the microfluidic chip 10, and the first branch channel 3221 is connected to one of the side planes of the separation chamber 302, which is a surface connecting the arc surface of the separation chamber 302 close to the rotation center 11 and the arc surface away from the rotation center 11.

[0085] The separation cavity 302 preferably has a relatively wide width in the radial direction of the microfluidic chip 10 , which is beneficial to improving the degree of separation of the sample.

[0086] In one example, in each distribution reaction structure 330, the separation chamber 302 is connected to the waste liquid storage chamber 310 through the fifth microfluidic channel 325 of area A. Excess liquid in the separation chamber 302 can be discharged into the waste liquid storage chamber 310 through the fifth microfluidic channel 325 of area A. Furthermore, the fifth microfluidic channel 325 of area A is a U-shaped microfluidic channel, and the opening of the fifth microfluidic channel 325 of area A faces away from the rotation center 11. Preferably, the fifth microfluidic channel 325 of area A is led out from the side of the separation chamber 302 close to the rotation center 11, which is conducive to the discharge of separated waste into the waste liquid storage chamber 310. Further preferably, the fifth microfluidic channel 325 of area A is led out from one end of the side of the separation chamber 302 close to the rotation center 11, which is conducive to the discharge of separated waste into the waste liquid storage chamber 310. In the specific example shown in the figure, the connection points of the second microfluidic channel 322 of area A and the fifth microfluidic channel 325 of area A on the separation chamber 302 are located at opposite ends of the separation chamber 302.

[0087] The zone A distribution cavity 304 preferably has a narrower width than the separation cavity 302 in the radial direction of the microfluidic chip 10 , which is conducive to filling the separation cavity 302 with the separated plasma sample and improving the uniformity of the amount of sample transported by the zone A distribution cavity 304 to the multiple reaction cavities 306 .

[0088] In one example, the zone A distribution cavity 304 extends along the rotational circumference of the microfluidic chip 10. Preferably, the radial width of the zone A distribution cavity 304 remains consistent in its extension direction.

[0089] In one example, in each distribution reaction structure 330, the zone A distribution chamber 304 is connected to the waste liquid storage chamber 310 through the zone A sixth microfluidic channel 326. Excess liquid in the zone A distribution chamber 304 can be discharged into the waste liquid storage chamber 310 through the zone A sixth microfluidic channel 326. Furthermore, the zone A sixth microfluidic channel 326 is a U-shaped microfluidic channel, and the opening of the zone A sixth microfluidic channel 326 faces away from the rotation center 11. Preferably, the zone A sixth microfluidic channel 326 is drawn out from the side of the zone A distribution chamber 304 near the rotation center 11. Further preferably, the zone A sixth microfluidic channel 326 is drawn out from one end of the side of the separation chamber 302 near the rotation center 11, which facilitates the discharge of excess liquid into the waste liquid storage chamber 310.

[0090] In the illustrated example, the fifth microfluidic channel 325 and the sixth microfluidic channel 326 of Area A converge and connect to one end of the seventh microfluidic channel 327 of Area A. The other end of the seventh microfluidic channel 327 of Area A is connected to the waste liquid storage chamber 310. A backflow prevention valve 312 is provided on the seventh microfluidic channel 327 of Area A. The backflow prevention valve 312 prevents waste liquid in the waste liquid storage chamber 310 from flowing back.

[0091] Preferably, in the distribution reaction structure 330 , the plurality of reaction units 332 are evenly distributed along the extension direction of the zone A distribution cavity 304 .

[0092] In one example, the reaction unit 332 further includes a first reagent inlet 308 in zone A and an eighth microfluidic channel 328 in zone A. The first reagent inlet 308 in zone A communicates with the reaction chamber 306 through the eighth microfluidic channel 328. The first reagent inlet 308 in zone A is closer to the rotation center 11 than the reaction chamber 306.

[0093] In the above example, required reagents can be added into the reaction chamber 306 through the first reagent inlet 308 in zone A.

[0094] Please further combine Figure 6 and Figure 9 In one example, the microfluidic chip 10 further includes a common reagent layer 200. The sample solvent addition layer 100, the common reagent layer 200, and the reaction layer 300 are stacked in sequence. The common reagent layer 200 can be used to store reagents required for multiple tests in the reaction layer 300.

[0095] The common reagent layer 200 has a zone B sample addition hole 204, a common reagent storage chamber 202, a zone B solvent addition hole 201, a zone B distribution chamber 207, and a diversion unit 230. The zone B solvent addition hole 201 is connected to the common reagent storage chamber 202. The common reagent storage chamber 202 is connected to the zone B distribution chamber 207. The zone B distribution chamber 207 is arranged around the rotation center 11. The diversion unit 230 includes a zone B first microchannel 221 and a zone B first connection port 209. The zone B first connection port 209 is connected to the zone B distribution chamber 207 through the zone B first microchannel 221. There are multiple diversion units 230. The multiple diversion units 230 are distributed along the extension direction of the zone B distribution chamber 207. The distances between the common reagent storage chamber 202, the zone B distribution chamber 207, and the diversion unit 230 and the rotation center 11 increase in sequence.

[0096] The plurality of diversion units 230 correspond one-to-one to the plurality of reaction units 332 in the reaction layer 300. The first connection port 209 in zone B communicates with the first reagent inlet 308 in zone A via a fifth interlayer channel (not shown). The sample addition port 204 in zone B communicates with the sample addition port 301 in zone A via a sixth interlayer channel (not shown).

[0097] In the above example, the sample liquid is added through the Section B sample addition well 204, enters the Section A sample addition well 301 in the reaction layer 300 via the sixth interlayer channel, and then enters the separation chamber 302. The common reagent storage chamber 202 can store the required reagents. During centrifugation, the reagents stored in the common reagent storage chamber 202 enter the Section B distribution chamber 207, then pass through the Section B first microfluidic channel 221 to the Section B first connection port 209, and then through the fifth interlayer channel to the Section A first reagent inlet 308 in the reaction layer 300, and then enter the reaction chamber 306 via the Section A eighth microfluidic channel 328.

[0098] In one example, the first micro-fluidic valve 208 of zone B is provided on the first micro-fluidic channel 221 of zone B.

[0099] like Figure 2 As shown in one example, the reaction unit 332 further includes a second reagent inlet 307 in zone A and a ninth microfluidic channel 329 in zone A. The second reagent inlet 307 in zone A is connected to the reaction chamber 306 through the ninth microfluidic channel 329 in zone A. The second reagent inlet 307 in zone A is closer to the rotation center 11 than the reaction chamber 306.

[0100] In the above example, required reagents can be added into the reaction chamber 306 through the second reagent inlet 307 in zone A.

[0101] In one example, the zone B distribution chamber 207 is in a circular shape with the microfluidic chip 10 as the center.

[0102] In one example, the common reagent storage chamber 202 stores a first reagent.

[0103] Optionally, the first reagent may be a single reagent or may include multiple reagents. The first reagent stored in the common reagent storage chamber 202 may be a fluid or a freeze-dried form.

[0104] Freeze-drying can be achieved by storing the first reagent in fluid form in the common reagent storage chamber 202 and then performing freeze-drying treatment.

[0105] Freeze-drying involves freezing a wet material below its freezing point (eutectic point), then sublimating and drying it under appropriate vacuum conditions to remove ice crystals. After sublimation, the material undergoes desorption and drying to remove some bound water. Freeze-dried products offer the following advantages: Because they are dried under low-temperature vacuum conditions, their decomposition rate is very low and their purity is high. They essentially maintain the volume of the original solution at the time of freezing, resulting in a porous, aesthetically pleasing appearance and uniform color. They dissolve readily in water, instantly restoring their original pharmaceutical properties. They minimize contamination and foreign matter, improving drug solubility and enhancing the clarity of the preparation. With a moisture content below 8%, freeze-dried products can be stored for long periods and are easily transported.

[0106] like Figure 10 As shown, in one example, the common reagent storage chamber 202 includes multiple sub-storage chambers. The multiple sub-storage chambers are distributed and spaced apart around the rotation center 11, and each sub-storage chamber is connected to the zone B distribution chamber 207. By providing multiple sub-storage chambers, multiple reagents can be delivered to the reaction chamber 306.

[0107] Please combine Figures 6 to 14 In one example, each of the multiple sub-storage chambers communicates with the zone B distribution chamber 207 via a reagent output channel 260. The reagent output channel 260 includes a first centrifugal flow channel 261 that extends from the common reagent storage chamber 202 and away from the rotation center 11. The first centrifugal flow channel 261 has a first discharge microfluidic valve 262 to lock the reagent in the sub-storage chamber when there is no centrifugal force.

[0108] like Figure 10 and Figure 12As shown, in one example, the reagent output channel 260 corresponding to at least one of the multiple sub-storage chambers further includes a delay unit 263. The delay unit 263 includes a first diverting channel 2631, a capillary force channel 2632, a second diverting channel 2633, and a second centrifugal force channel 2634. The first centrifugal force channel 261 extends away from the rotation center 11 after exiting the common reagent storage chamber 202 and is connected to one end of the first diverting channel 2631. The other end of the first diverting channel 2631 is connected to one end of the capillary force channel 2632. The capillary force channel 2632 extends from the first diverting channel 2631 to near the rotation center 11. The other end of the capillary force channel 2632 is connected to one end of the second diverting channel 2633. The other end of the second diverting channel 2633 is connected to the second centrifugal force channel 2634. The second centrifugal force channel 2634 extends away from the rotation center 11 after exiting the second diverting channel 2633. The second centrifugal flow channel 2634 has a second discharge micro-flow valve 2635 .

[0109] The delay unit 263 in the aforementioned microfluidic chip 10 can delay the time it takes for a reagent to be discharged from its corresponding sub-storage chamber to other fluid paths outside the reagent output channel 260. During testing, by increasing the centrifugal speed, the reagent in the sub-storage chamber breaks through the first centrifugal flow channel 261 on the first centrifugal flow channel 261 and enters the first diverting flow channel 2631 of the delay unit 263. At this point, because the centrifugal force is greater than the capillary force, the reagent is temporarily retained in the first diverting flow channel 2631. By decreasing the centrifugal speed, the centrifugal force becomes less than the capillary force, and the reagent flows from the first diverting flow channel 2631 into the capillary flow channel 2632, then into the second diverting flow channel 2633, reaching the second discharge microfluidic valve 2635 on the second centrifugal flow channel 2634. By increasing the centrifugal speed, the reagent breaks through the second discharge microfluidic valve 2635 and is discharged from the second centrifugal flow channel 2634. In this way, after several cycles of varying centrifugal speeds, the reagent can be discharged through the reagent output channel 260. The microfluidic chip 10 is suitable for reactions requiring controlled timing and sequence of reagent delivery, particularly reactions requiring the sequential application of multiple reagents. The chip can control the timing of multiple reagent application to perform multi-step reactions. Therefore, the microfluidic chip 10 described above can perform relatively complex reactions while avoiding operational complexity during the experiment.

[0110] like Figure 14 As shown, in one example, the reagent output channel 260 corresponding to at least one of the multiple sub-storage chambers has multiple delay units 263, and the multiple delay units 263 are connected in sequence.

[0111] Furthermore, in one example, the number of delay units 263 in the reagent output channel 260 corresponding to each sub-storage chamber is different from that in the reagent output channels 260 corresponding to other sub-storage chambers.

[0112] In the illustrated example, the common reagent storage chamber 202 includes a first sub-storage chamber 2021, a second sub-storage chamber 2022, and a third sub-storage chamber 2023. The number of delay units 263 in the reagent output channel 260 corresponding to the first, second, and third sub-storage chambers 2021, 2022, and 2023 increases in sequence. More specifically, the number of delay units 263 in the reagent output channel 260 corresponding to the first sub-storage chamber 2021 is zero, the number of delay units 263 in the reagent output channel 260 corresponding to the second sub-storage chamber 2022 is one, and the number of delay units 263 in the reagent output channel 260 corresponding to the third sub-storage chamber 2023 is two. The first sub-storage chamber 2021 stores a cleaning agent, the second sub-storage chamber 2022 stores a pre-initiator, and the third sub-storage chamber 2023 stores an initiator. This allows for precise control of the order in which the cleaning agent, pre-initiator, and initiator are delivered to the reaction chamber 306.

[0113] It can be understood that if the second reagent does not need to be added, the sample solvent adding layer 100 can be omitted.

[0114] In one example, a third reagent is stored in the reaction chamber 306 .

[0115] In one example, the reaction chamber 306 stores magnetic beads labeled with immune components, which are antigens or antibodies and can be freeze-dried. For example, the reaction chamber 306 stores freeze-dried CTNI / NT-pro BNP / D-dimer / MYO / CKMB-coated magnetic beads. The magnetic beads can be fixed by magnetic force to remain in the reaction chamber 306 to prevent them from being thrown into the waste liquid storage chamber 310. In one example, the reaction layer 300 includes a bottom plate and a slot plate, which is connected to the common reagent layer 200. The bottom plate and the slot plate are docked to form a cavity structure in the reaction layer 300. During manufacturing, the antigen-coated magnetic beads can be fixed to the bottom plate by dispensing glue, and then the rest of the slot plate is docked with the slot plate.

[0116] Material storage chamber 109 stores corresponding chemically labeled antibodies, which can be freeze-dried. For example, freeze-dried acridine-labeled antibodies for CTNI / NT-pro BNP / D-dimer / MYO / CKMB are available. The chemically labeled antibodies enter reaction chamber 306 and react with the antigens on the magnetic beads. After incubation, an antibody-antigen-labeled antibody structure is formed.

[0117] The cleaning agent may include a surfactant to wash away unbound antibodies and discharge them into the waste liquid storage chamber 310. The pre-excitation solution may be NaOH, and the excitation solution may be hydrogen peroxide. The labeled antibody is labeled with an acridinium ester, which will emit light in the pre-excitation solution and excitation solution system, so that the luminescent signal can be detected.

[0118] The extending direction of the first turning channel 2631 and the second turning channel 2633 is the same as or close to the rotation direction of the microfluidic chip 10. The first turning channel 2631 and the second turning channel 2633 can be, but are not limited to, arc-shaped, straight-line-shaped, etc.

[0119] In one example, the first diverting channel 2631 is an arc with the rotation center 11 as the center.

[0120] In one example, the second turning channel 2633 is an arc with the rotation center 11 as the center.

[0121] The common reagent layer 200 in the microfluidic chip 10 of the above example is used to store common reagents for multi-sample and multi-item testing, and is capable of performing complex multi-step chemical reactions. It can greatly increase the complexity of chemical reactions in the microfluidic chip 10, expand the categories of chemical reactions in the microfluidic chip 10, and improve the use value of the microfluidic chip 10 in the POCT field.

[0122] Traditional chips often require external reagents, resulting in bulky supporting instruments, making it difficult to meet the needs of multi-sample and multi-project testing, and also unable to achieve the results of orderly release of reagents integrated into the chip. In addition, the microfluidic chip 10 described above does not require the addition of common reagents during experiments. The reagents can be automatically and orderly released on the chip, thereby reducing the number of supporting instruments and facilitating the detection of multiple samples and multi-projects.

[0123] It can be understood that if there is no need to add a common reagent (first reagent), the common reagent layer 200 can be omitted.

[0124] In the common reagent layer 200, the flow diversion unit 230 further includes a second connection port 210 in area B, a second microfluidic channel 211 in area B, and a third connection port 212 in area B. The second connection port 210 in area B is connected to the third connection port 212 in area B via the second microfluidic channel 211. The second connection port 210 in area B is further away from the rotation center 11 than the third connection port 212 in area B.

[0125] The multiple reagent storage structures 130 in the sample solvent addition layer 100 correspond one-to-one to the multiple diversion units 230 in the common reagent layer 200. The sample addition hole 103 in Area C is connected to the sample loading hole 201 via a first interlayer channel (not shown). The first connection port 104 in Area C is connected to the solvent addition hole 201 in Area B via a second interlayer channel (not shown). The second connection port 111 in Area C is connected to the second connection port 210 in Area B via a third interlayer channel (not shown). The third connection port 212 in Area B is connected to the second reagent inlet 307 in Area A via a fourth interlayer channel (not shown).

[0126] In the above example, the sample liquid is added from the sample addition hole 103 in zone C, reaches the sample addition hole 201 in the common reagent layer 200 through the first interlayer channel, then enters the sample addition hole 301 in zone A in the reaction layer 300 through the sixth interlayer channel, and then enters the separation chamber 302. The solvent is added from the solvent addition hole 101 of area C. One path of the solvent enters the distribution chamber 107 of area C through the first microchannel 121 of area C, then enters the material storage chamber 109 through the third microchannel, and then reaches the second connection port 111 of area C through the fourth microchannel 124 of area C, and then reaches the second connection port 210 of area B in the common reagent layer 200 through the third interlayer channel, and then reaches the third connection port 212 of area B through the second microchannel 211 of area B, and then reaches the second reagent inlet 307 of area A in the reaction layer 300 through the fourth interlayer channel, and enters the reaction chamber 306 through the ninth microchannel 329 of area A, providing solvent for the material stored in the reaction chamber 306; another path of the solvent reaches the first connection port 104 of area C through the second microchannel 122 of area C, and then enters the common reagent storage chamber 202 through the second interlayer channel and the solvent addition hole 201 of area B, providing solvent for the material stored in the common reagent storage chamber 202.

[0127] In one example, the microfluidic chip 10 further includes a second vent 402. One end of the second vent 402 is connected to the common reagent storage chamber 202, and the other end is open to a side surface of the microfluidic chip 10. More specifically, the common reagent storage chamber 202 includes a connection port 203, and the second vent 402 is connected to the common reagent storage chamber 202 through the connection port 203. The second vent 402 can be used to add reagents to the common reagent storage chamber 202 and can also serve as ventilation during the experiment.

[0128] In one example, the second microchannel 211 in area B is a U-shaped microchannel, and the opening of the second microchannel 211 in area B faces away from the rotation center 11 .

[0129] In one example, the sample solvent addition layer 100, the common reagent layer 200, and the reaction layer 300 are integrally connected. In other examples, the sample solvent addition layer 100, the common reagent layer 200, and the reaction layer 300 can also be independently manufactured and then connected.

[0130] The present invention will be further described below by taking a method for performing detection using the illustrated specific example of the microfluidic chip 10 as an example.

[0131] A microfluidic chip 10 according to a specific example of the present invention includes a sample solvent addition layer 100 , a common reagent layer 200 , and a reaction layer 300 , which are stacked in sequence.

[0132] The microfluidic chip 10 includes a sample solvent addition layer 100 , a common reagent layer 200 , and a reaction layer 300 , which are stacked.

[0133] Sample solvent added layer 100:

[0134] The sample solvent addition layer 100 includes a Zone C sample addition port 103, a Zone C solvent addition port 101, a Zone C first microfluidic channel 121, a Zone C distribution chamber 107, a Zone C second microfluidic channel 122, a Zone C first connection port 104, and a reagent storage structure 130. The Zone C distribution chamber 107 is arranged around the rotation center 11. The Zone C solvent addition port 101 communicates with the Zone C distribution chamber 107 via the Zone C first microfluidic channel 121. A Zone C first microfluidic valve 105 is provided on the Zone C first microfluidic channel 121. The Zone C solvent addition port 101 communicates with the Zone C first connection port 104 via the Zone C second microfluidic channel 122. A Zone C second microfluidic valve 106 is provided on the Zone C second microfluidic channel 122.

[0135] The reagent storage structure 130 includes the third microfluidic channel 123 of Section C, the material storage chamber 109, the fourth microfluidic channel 124 of Section C, the second connecting port 111 of Section C, the fifth microfluidic channel 125 of Section C, and a third vent 112. The material storage chamber 109 communicates with the distribution chamber 107 of Section C via the third microchannel. A third microfluidic valve 108 of Section C is provided on the third microfluidic channel 123 of Section C. The material storage chamber 109 communicates with the second connecting port 111 of Section C via the fourth microfluidic channel 124 of Section C. A fourth microfluidic valve 110 of Section C is provided on the fourth microfluidic channel 124 of Section C.

[0136] One end of the fifth microchannel 125 of area C is connected to the third microchannel 123 of area C, and the other end is connected to the third vent 112. The third vent 112 opens on one side surface of the microfluidic chip 10.

[0137] There are multiple reagent storage structures 130, distributed along the extension direction of the area C distribution chamber 107. The area C distribution chamber 107 includes multiple sub-distribution chambers, which are distributed and spaced apart around the rotation center 11. Each sub-distribution chamber is connected to multiple reagent storage structures 130.

[0138] The distances between the zone C solvent addition hole 101, the zone C distribution chamber 107, the material storage chamber 109 and the zone C second connection port 111 and the rotation center 11 increase in sequence. The zone C first connection port 104 is further away from the rotation center 11 than the zone C solvent addition hole 101.

[0139] The reagent storage structure 130 also includes a reagent addition slot 113, a feed permeation hole 114, a feed microfluidic channel 115, a first feed microfluidic valve 116, and a second feed microfluidic valve 117. The reagent addition slot 113 opens to a side surface of the microfluidic chip 10. The reagent addition slot 113 is connected to the feed microfluidic channel 115 through the feed permeation hole 114. The feed microfluidic channel 115 is connected to the material storage chamber 109 through the first feed microfluidic valve 116. The material storage chamber 109 is further away from the rotation center 11 than the reagent addition port. The feed permeation hole 114 is connected to the feed microfluidic channel 115 through the second feed microfluidic valve 117.

[0140] One end of the feed infiltration hole 114, which is in communication with the reagent addition tank 113, opens to the bottom of the reagent addition tank 113. The feed infiltration hole 114 extends perpendicular to the surface of the microfluidic chip 10. One end of the first feed microfluidic valve 116, which is in communication with the material storage chamber 109, opens to the side wall of the material storage chamber 109.

[0141] The first feed microfluidic valve 116 includes a first section 1161, a second section 1162, and a third section 1163, which are sequentially connected. One end of the first section 1161 is connected to the feed microfluidic channel 115, and one end of the third section 1163 is connected to the material storage chamber 109. The first section 1161 and the third section 1163 extend away from the rotation center 11, with the third section 1163 being further away from the rotation center 11 than the first section 1161. The first section 1161 and the third section 1163 are each perpendicular to the second section 1162.

[0142] The material storage chamber 109 stores freeze-dried acridine-labeled CTNI / NT-pro BNP / D-dimer / MYO / CKMB antibodies.

[0143] Common reagent layer 200:

[0144] The common reagent layer 200 includes a Section B sample addition port 204, a common reagent storage chamber 202, a Section B solvent addition port 201, a second-first microfluidic channel, a Section B distribution chamber 207, and a diversion unit 230. The Section B solvent addition port 201 communicates with the common reagent storage chamber 202. The common reagent storage chamber 202 communicates with the Section B distribution chamber 207 via the second-first microfluidic channel. The Section B distribution chamber 207 is arranged around the rotation center 11.

[0145] The diversion unit 230 includes a first microfluidic channel 221 in Area B, a first connection port 209 in Area B, a second connection port 210 in Area B, a second microfluidic channel 211 in Area B, and a third connection port 212 in Area B. The first connection port 209 in Area B is connected to the distribution chamber 207 in Area B through the first microfluidic channel 221 in Area B. A first microfluidic valve 208 in Area B is provided on the first microfluidic channel 221 in Area B. The second connection port 210 in Area B is connected to the third connection port 212 in Area B through the second microfluidic channel 211 in Area B. The second microfluidic channel 211 in Area B is a U-shaped microfluidic channel. The opening of the second microfluidic channel 211 in Area B faces away from the rotation center 11. The second connection port 210 in Area B is farther away from the rotation center 11 than the third connection port 212 in Area B.

[0146] There are multiple diversion units 230. The multiple diversion units 230 are distributed along the extension direction of the zone B distribution chamber 207. The distances between the common reagent storage chamber 202, the zone B distribution chamber 207 and the diversion units 230 and the rotation center 11 increase in sequence.

[0147] The common reagent storage chamber 202 includes a first sub-storage chamber 2021 , a second sub-storage chamber 2022 and a third sub-storage chamber 2023 . The multiple sub-storage chambers are distributed around the rotation center 11 and spaced apart, and each sub-storage chamber is connected to the zone B distribution chamber 207 .

[0148] Each of the multiple sub-storage chambers is connected to the zone B distribution chamber 207 via a reagent output channel 260. The reagent output channel 260 includes a first centrifugal flow channel 261, which extends from the common reagent storage chamber 202 and away from the rotation center 11. The first centrifugal flow channel 261 has a first discharge microfluidic valve 262.

[0149] The reagent output channel 260 also includes a delay unit 263. The delay unit 263 includes a first diverting channel 2631, a capillary channel 2632, a second diverting channel 2633, and a second centrifugal channel 2634. The first centrifugal channel 261 extends away from the rotation center 11 after exiting the common reagent storage chamber 202 and is connected to one end of the first diverting channel 2631. The other end of the first diverting channel 2631 is connected to one end of the capillary channel 2632. The capillary channel 2632 extends from the first diverting channel 2631 to near the rotation center 11. The other end of the capillary channel 2632 is connected to one end of the second diverting channel 2633. The other end of the second diverting channel 2633 is connected to the second centrifugal channel 2634. The second centrifugal channel 2634 extends away from the rotation center 11 after exiting the second diverting channel 2633. The second centrifugal flow channel 2634 has a second discharge micro-flow valve 2635 .

[0150] The number of delay units 263 in the reagent output channel 260 corresponding to the first sub-storage chamber 2021 is zero, the number of delay units 263 in the reagent output channel 260 corresponding to the second sub-storage chamber 2022 is one, and the number of delay units 263 in the reagent output channel 260 corresponding to the third sub-storage chamber 2023 is two.

[0151] The first sub-storage chamber 2021 stores freeze-dried cleaning agent, the second sub-storage chamber 2022 stores freeze-dried pre-stimulator, and the third sub-storage chamber 2023 stores freeze-dried stimulator.

[0152] Reaction layer 300:

[0153] The reaction layer 300 has a plurality of distribution reaction structures 330. The plurality of distribution reaction structures 330 are distributed around the rotation center 11 and are spaced apart.

[0154] The distribution reaction structure 330 includes a region A sample addition well 301, a separation chamber 302, a region A second microfluidic channel 322, a region A distribution chamber 304, a reaction unit 332, a region A fourth microfluidic channel 324, and a waste liquid storage chamber 310. The region A sample addition well 301 communicates with the separation chamber 302 via the region A third microfluidic channel 323. The separation chamber 302 communicates with the region A distribution chamber 304 via the region A second microfluidic channel 322.

[0155] Reaction unit 332 includes a first microfluidic channel 321 in Section A, a reaction chamber 306, an eighth microfluidic channel 328 in Section A, a first reagent inlet 308 in Section A, a ninth microfluidic channel 329 in Section A, and a second reagent inlet 307 in Section A. Reaction chamber 306 communicates with distribution chamber 304 in Section A via first microfluidic channel 321. Reaction chamber 306 communicates with waste liquid storage chamber 310 via fourth microfluidic channel 324 in Section A. First reagent inlet 308 in Section A communicates with reaction chamber 306 via eighth microfluidic channel 328 in Section A. Magnetic beads coated with CTNI / NT-pro BNP / D-dimer / MYO / CKMB are stored in reaction chamber 306. First reagent inlet 308 in Section A is located closer to rotation center 11 than reaction chamber 306. Second reagent inlet 307 in Section A communicates with reaction chamber 306 via ninth microfluidic channel 329 in Section A. The second reagent inlet 307 of zone A is closer to the rotation center 11 than the reaction chamber 306 .

[0156] In each distribution reaction structure 330, the separation chamber 302 is a sector-shaped annular cavity extending along the circumferential direction of rotation of the microfluidic chip 10. Each distribution reaction structure 330 has multiple reaction units 332, evenly distributed along the extension of the zone A distribution chamber 304. The separation chamber 302, zone A distribution chamber 304, reaction chamber 306, and waste liquid storage chamber 310 are positioned at increasing distances from the rotation center 11.

[0157] The second microchannel 322 in zone A includes a first branch channel 3221, a second branch channel 3222, and a third branch channel 3223, which are sequentially connected. The first branch channel 3221 extends from the separation chamber 302, near the rotation center 11, and connects to one end of the second branch channel 3222. The other end of the second branch channel 3222 connects to the third branch channel 3223. The third branch channel 3223 extends from the second branch channel 3222, away from the rotation center 11, and connects to the zone A distribution chamber 304. The first branch channel 3221 is connected to a first side surface 3021-3021 of the separation chamber 302. The first side surface 3021-3021 connects the second side surface 3022-3022 of the separation chamber 302, which is close to the rotation center 11, and the third side surface 3023-3023, which is away from the rotation center 11.

[0158] In each distribution reaction structure 330, the separation chamber 302 communicates with the waste liquid storage chamber 310 via the fifth microfluidic channel 325 in Section A. The fifth microfluidic channel 325 in Section A is a U-shaped microfluidic channel, with its opening facing away from the rotation center 11. The fifth microfluidic channel 325 in Section A extends from one end of the second side surface 30223022 of the separation chamber 302, which is located near the rotation center 11. The connection points between the second microfluidic channel 322 and the fifth microfluidic channel 325 in Section A are located at opposite ends of the separation chamber 302.

[0159] The zone A distribution cavity 304 extends along the rotational circumference of the microfluidic chip 10. The radial width of the zone A distribution cavity 304 remains consistent along the extension direction. The zone A distribution cavity 304 has a narrower width than the separation cavity 302 in the radial direction of the microfluidic chip 10.

[0160] In each distribution reaction structure 330 , the zone A distribution chamber 304 is connected to the waste liquid storage chamber 310 via the zone A sixth microchannel 326 . The zone A sixth microchannel 326 is led out from one end of the side of the separation chamber 302 close to the rotation center 11 .

[0161] The fifth microchannel 325 and the sixth microchannel 326 of area A merge and communicate with one end of the seventh microchannel 327 of area A, and the other end of the seventh microchannel 327 of area A communicates with the waste liquid storage chamber 310. An anti-backflow valve 312 is provided on the seventh microchannel 327 of area A.

[0162] The fourth micro-channel 324 of zone A is provided with a second micro-flow valve 309 of zone A.

[0163] The first micro-channel 321 of zone A is provided with a first micro-flow valve 305 of zone A.

[0164] The multiple reagent storage structures 130 in the sample solvent addition layer 100 correspond one-to-one with the multiple diversion units 230 in the common reagent layer 200. The sample addition well 103 in Area C communicates with the sample loading well 201 via a first interlayer channel. The first connection port 104 in Area C communicates with the solvent addition well 201 in Area B via a second interlayer channel. The second connection port 111 in Area C communicates with the second connection port 210 in Area B via a third interlayer channel. The third connection port 212 in Area B communicates with the second reagent inlet 307 in Area A via a fourth interlayer channel.

[0165] The multiple flow diversion units 230 in the common reagent layer 200 correspond one-to-one to the multiple reaction units 332 in the reaction layer 300. The first connection port 209 in zone B is connected to the first reagent inlet 308 in zone A via the fifth interlayer channel. The sample addition hole 204 in zone B is connected to the sample addition hole 301 in zone A via the sixth interlayer channel.

[0166] The microfluidic chip 10 further includes a second vent 402 and a first vent 401. One end of the second vent 402 is connected to the common reagent storage chamber 202, and the other end is open to a side surface of the microfluidic chip 10. One end of the first vent 401 is connected to the waste liquid storage chamber 310, and the other end is open to a side surface of the microfluidic chip 10.

[0167] The method for performing detection using the microfluidic chip 10 of the above specific example includes the following steps:

[0168] (1) Add the whole blood sample to be tested to the sample addition hole 103 of the zone C of the sample solvent addition layer (1). Add the above-mentioned microfluidic chip 10 to the matching centrifuge. Add pure water to the zone C solvent addition hole 101. Start rotation, and the sample reaches the separation chamber 302 of the reaction layer 300 through the first interlayer channel, the sixth interlayer channel and the third microchannel 323 of zone A for separation. The pure water reaches the second microfluidic valve 106 of zone C between the zone C solvent addition hole 101 and the first connection port 104 of zone C, and the first microfluidic valve 105 of zone C between the zone C solvent addition hole 101 and the zone C distribution chamber 107.

[0169] (2) The centrifugal speed is increased, and the pure water breaks through the first microfluidic valve 105 and the second microfluidic valve 106 of area C. The centrifugal speed is reduced, and the plasma separated from the whole blood sample passes through the second microfluidic channel 322 of area A under the action of capillary force and enters the distribution chamber 304 of area A. The pure water enters the distribution chamber 107 of area C of the sample solvent addition layer (1) and the common reagent storage chamber 202 in the common reagent layer 200. The freeze-dried cleaning solution, freeze-dried pre-excitation solution, and freeze-dried excitation solution stored in the three common reagent storage chambers 202 are dissolved in water.

[0170] 3. The centrifugal speed is increased, and the pure water flows through the third microfluidic valve 108 in zone C behind the zone C distribution chamber 107 in the sample solvent addition layer 100 and enters the chemically labeled antibody unit 109. The lyophilized chemically labeled antibodies in the chemically labeled antibody unit 109 dissolve in water. The plasma flows through the first microfluidic valve 305 in zone A behind the zone A distribution chamber 304 in the reaction layer 300 and enters the reaction chamber 306.

[0171] (4) Variable speed centrifugation: the chemical substance labeled antibody unit 109, the common reagent unit 202 and the reaction chamber 306 are mixed at the same time.

[0172] (5) The centrifugal speed is increased, and the chemically labeled antibodies break through the fourth microfluidic valve 110 in zone C behind the chemically labeled antibody unit 109, and enter the second microfluidic channel 211 in zone B through the second connection port 111 in zone C and the second connection port 210 in zone B in the common reagent layer 200. The centrifugal speed is decreased, and the chemically labeled antibodies pass through the second microfluidic channel 211 in zone B and the third connection port 212 in zone B to reach the reaction chamber 306 in the reaction layer 300.

[0173] (6) Variable speed centrifugation, the chemical substance labeled antibodies in the reaction chamber 306 are mixed with the plasma and magnetic beads.

[0174] (7) The centrifugal speed is increased. The mixture in the reaction chamber 306 passes through the second microfluidic valve 309 in the area A behind the reaction chamber 306 and reaches the waste liquid storage chamber 310. This indicates that the reaction chamber 306 is now full of reagents, and the cleaning liquid in the common reagent layer 200 reaches the area B distribution chamber 207. The centrifugal speed is reduced, and the cleaning liquid fills the area B distribution chamber 207.

[0175] (8) Increase the centrifugal speed. The cleaning liquid reaches the reaction chamber 306 through the first connecting port 209 of zone B, the fifth interlayer channel, and the first reagent inlet 308 of zone A. The pre-excitation liquid reaches the distribution chamber 207 of zone B. Reduce the centrifugal speed. The pre-excitation liquid fills the distribution chamber 207 of zone B.

[0176] (9) Increase the centrifugal speed, the cleaning liquid reaches the waste liquid storage chamber 310, the pre-excitation liquid reaches the reaction chamber 306, and the excitation liquid reaches the zone B distribution chamber 207. Reduce the centrifugal speed, and the excitation liquid fills the zone B distribution chamber 207.

[0177] (10) Increase the centrifugal speed until the excitation liquid reaches the reaction chamber 306, and centrifuge at a variable speed to mix.

[0178] (11) Chemiluminescence captures the light signal, reads the value, and completes the detection.

[0179] The rotation speed required in the above steps can be 100-10000 r / min, such as 500 r / min, 1000 r / min, 2000 r / min, etc. The rotation time can be 1 s-10 min, such as 10 s, 30 s, 1 min, 5 min, etc.

[0180] The microfluidic chip 10 of this specific example integrates the detection of multiple items of multiple samples. During the detection, the tester only needs to add the blood sample to the sample well. After being put on the machine, the machine will uniformly add the reconstitution solvent. The instrument automatically performs multiple processes such as reagent reconstitution, mixing, centrifugation, constant volume, reaction, elution, and luminescence.

[0181] The above-mentioned microfluidic chip 10 has a reagent storage function and can pre-store reagents before the detection test. When pre-storing reagents, the reagents that need to be pre-stored are added to the reagent addition groove 113, and the reagents enter the feed microchannel 115 through the feed penetration hole 114. Through rotation and centrifugation, the reagents break through the first feed microfluidic valve 116 and enter the material storage chamber 109. The first feed microfluidic valve 116 can prevent the reagent from flowing back from the material storage chamber 109, thereby realizing the storage of the reagents. The above-mentioned microfluidic chip 10 encapsulates the reagents required for detection into the microfluidic chip 10 through structural design. It can be understood that the material storage chamber 109 can be connected to the detection liquid circuit. When the detection is required, the reagent is driven into the detection liquid circuit by increasing the centrifugal force. The above-mentioned microfluidic chip 10 realizes the microfluidic project detection of the reagent chip integration, which can save the operation of adding corresponding reagents in the test link, is easy to use, and saves time.

[0182] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0183] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A microfluidic chip, characterized in that: It has a reagent storage structure, which includes a reagent adding tank, a feed infiltration hole, a feed microchannel, a first feed microflow valve and a material storage cavity; The reagent addition tank is opened on one side surface of the microfluidic chip, and the reagent addition tank is connected to the feed microchannel through the feed penetration hole, and the feed microchannel is connected to the material storage chamber through the first feed microfluidic valve; the microfluidic chip has a rotation center, and the material storage chamber is further away from the rotation center than the reagent addition tank; the first feed microfluidic valve includes a first section, a second section and a third section that are connected in sequence, one end of the first section is connected to the feed microchannel, and one end of the third section is connected to the material storage chamber, the first section and the third section extend away from the rotation center, the third section is further away from the rotation center than the first section, and the first section and the third section are respectively perpendicular to the second section; the microfluidic chip also includes a solvent addition hole in area C; the reagent storage structure also includes a third microchannel in area C, the third microchannel in area C is connected to the material storage chamber, the solvent addition hole in area C is connected to the third microchannel in area C, and the material storage chamber is further away from the rotation center than the solvent addition hole in area C.

2. The microfluidic chip according to claim 1, wherein One end of the feed permeation hole opens at the bottom of the reagent adding tank to communicate with the reagent adding tank.

3. The microfluidic chip according to claim 2, wherein: The extending direction of the feed permeation hole is perpendicular to the disk surface of the microfluidic chip.

4. The microfluidic chip according to any one of claims 1 to 3, wherein: The reagent storage structure further includes a second feed microfluidic valve, and the feed permeation hole is communicated with the feed microfluidic channel through the second feed microfluidic valve.

5. The microfluidic chip according to any one of claims 1 to 3, wherein: The first feed micro-flow valve is a steam trap or a capillary valve.

6. The microfluidic chip according to claim 1, wherein There are multiple reagent storage structures, and the multiple reagent storage structures are distributed around the rotation center and arranged at intervals.

7. The microfluidic chip according to claim 6, wherein: The microfluidic chip also includes a first microchannel in area C and a distribution chamber in area C. The distribution chamber in area C is arranged around the rotation center. The distribution chamber in area C is connected to the solvent addition hole in area C through the first microchannel in area C. The distribution chamber in area C is connected to the material storage chamber through the third microchannel in area C. Multiple reagent storage structures are distributed along the extension direction of the distribution chamber in area C. The distances between the solvent addition hole in area C, the distribution chamber in area C and the material storage chamber and the rotation center increase successively.

8. The microfluidic chip according to claim 7, wherein: The zone C distribution chamber includes a plurality of sub-distribution chambers, which are distributed around the rotation center and spaced apart, and each of the sub-distribution chambers is connected to a plurality of the reagent storage structures.

9. The microfluidic chip according to claim 7, wherein: The microfluidic chip includes a sample solvent addition layer and a reaction layer arranged in a stacked manner; The sample solvent addition layer comprises a sample addition hole in area C, a solvent addition hole in area C, a first microfluidic channel in area C, a distribution cavity in area C, and the reagent storage structure. The reagent storage structure further comprises a fourth microfluidic channel in area C and a second connection port in area C. The material storage cavity is connected to the second connection port in area C via the fourth microfluidic channel in area C. The reaction layer has multiple distribution reaction structures, which are distributed and spaced around the rotation center; the distribution reaction structure includes a sample addition hole in area A, a distribution chamber in area A, and a reaction unit; the sample addition hole in area A is connected to the distribution chamber in area A, and the reaction unit includes a third microfluidic channel in area A and a reaction chamber, and the reaction chamber is connected to the distribution chamber in area A through the third microfluidic channel in area A; the distribution chamber in area A extends around the rotation center, and there are multiple reaction units in the distribution reaction structure, and the multiple reaction units are distributed along the extension direction of the distribution chamber in area A. The distribution chamber in area A is closer to the rotation center than the reaction chamber, the sample addition hole in area A is connected to the sample addition hole in area C, and the reaction chamber is connected to the second connection port in area C.

10. The microfluidic chip according to claim 9, wherein: The distribution reaction structure also includes a separation chamber and a second microchannel in area A. The separation chamber is connected to the distribution chamber in area A through the second microchannel in area A. The sample addition hole in area A is connected to the separation chamber through the first microchannel in area A. The separation chamber is closer to the rotation center than the distribution chamber in area A.

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