A biological fluid detection device, detection system and detection method

Abstract

The present invention discloses a biological fluid detection device, comprising: a chassis, including a mixing zone, a first partition group and a second partition group vertically protruding from the upper surface and concentrically arranged, and an active channel; a reaction zone, arranged along the outer periphery of the chassis, provided with a reaction membrane; a waste liquid zone, arranged along the outer periphery of the chassis, provided with a water-absorbing membrane; a first channel, used to connect the reaction zone and the mixing zone; a second channel, used to connect the waste liquid zone and the mixing zone; a cover plate, rotatably sealed and connected to the chassis, the cover plate forms a feed port corresponding to the mixing zone, and vertically protrudes from the lower surface to form a blocking member group, which can rotate in the active channel to connect or block the first channel and the second channel. The present invention also discloses a biological fluid detection system. The present invention further discloses a biological fluid detection method. The detection device of the present invention has a long shelf life, the entire detection process is simple and reasonable to control, the overall structure is simple and effective, and the manufacturing cost is relatively low.

Description

Technical Field

[0001] The present invention belongs to the field of biological detection technology, and in particular relates to a biological fluid detection device, a detection system and a detection method. Background Art

[0002] Biofluid testing has received more and more attention in the medical industry. It can qualitatively or quantitatively measure the changes in various biological indicators in human body fluids to provide consultation on disease diagnosis or treatment indicators, such as HbA1c testing or GA testing.

[0003] In order to solve the problem that the reagents and washing solutions or other reactants need to be mixed manually in the prior art, Chinese Patent No. 204816573 discloses a "biological sample reaction box" which can separate the seal and the reagent storage part by a push rod, thus reducing the steps of manual operation. However, the above structure is relatively complex and the manufacturing cost is relatively high.

[0004] Chinese patent CN 104641241B discloses a "biochemical analysis box with improved operability", in which a chamber for storing a reaction solution is arranged inside the reaction box, so that when the plug-in sample box is inserted into the reaction box, the reaction solution stored in the chamber is automatically supplied. However, the sample box and the reaction box need to be designed separately, which increases the complexity of use, and the structure is still complex and the manufacturing cost is high. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the present invention provides a biological fluid detection device, a detection system and a detection method with strong reaction controllability, a simple and rapid detection method and a simple structure.

[0006] The technical solution adopted by the present invention to solve its technical problem is:

[0007] A biological fluid detection device, comprising:

[0008] A chassis including a mixing area formed in the center, a first partition group and a second partition group vertically protruding from the upper surface and concentrically arranged, and a movable channel;

[0009] A reaction zone, arranged along the periphery of the chassis, provided with a reaction membrane;

[0010] The waste liquid area is arranged along the periphery of the bottom plate and is provided with a water absorbing membrane;

[0011] A first channel is arranged radially along the first partition group and is used to connect the reaction zone and the mixing zone;

[0012] A second channel is arranged radially along the first partition group and is used to connect the waste liquid area and the mixing area;

[0013] The cover plate is rotatably sealed and connected to the bottom plate. The cover plate forms a feed port corresponding to the mixing zone. A blocking member group is vertically protruded from the lower surface. The blocking member group can rotate in the movable channel to connect or block the first channel and the second channel.

[0014] Furthermore, it also includes a liquid storage area arranged on the outer periphery of the bottom plate, and a third channel for connecting the liquid storage area and the mixing area.

[0015] Furthermore, the blocking member group includes blocking member 1, blocking member 2 and blocking member 3 which are concentrically and spaced apart. Blocking member 1, blocking member 2 and blocking member 3 can rotate in the movable channel to connect or block the first channel, the second channel and the third channel.

[0016] Furthermore, the movable channel includes an annular space located between the outer wall of the first partition group and the inner wall of the second partition group, and a partial space close to the inner wall of the first partition group; the second and third blocking elements can rotate in the annular space to connect or block the second channel and the third channel, and the first blocking element can rotate in contact with the inner wall of the first partition group to connect or block the first channel.

[0017] Furthermore, the bottom surface of the liquid storage area is provided with an inclined surface to facilitate the eluent in the liquid storage area to flow to the mixing area.

[0018] Furthermore, the first partition group includes partition one, partition two and partition three which are concentrically arranged and spaced apart along the circumferential direction, and the second partition group includes partition four and partition five which are concentrically arranged and spaced apart along the circumferential direction.

[0019] Furthermore, the outer periphery of the chassis forms waste liquid tanks and liquid storage tanks arranged at intervals, the waste liquid area is detachably connected to the waste liquid tank, the liquid storage area is detachably connected to the liquid storage tank, and the reaction area is arranged between the first partition group and the second partition group.

[0020] Furthermore, the waste liquid tank is arranged between partition four and partition five, and the liquid storage tank is arranged between partition four and partition five.

[0021] Furthermore, the cover plate is provided with a positioning column which can be connected with an external power source.

[0022] The present invention also discloses a biological fluid detection system, comprising:

[0023] A chassis including a mixing area formed in the center, a first partition group and a second partition group vertically protruding from the upper surface and concentrically arranged, and a movable channel;

[0024] A reaction zone, arranged along the periphery of the chassis, provided with a reaction membrane;

[0025] The waste liquid area is arranged along the periphery of the bottom plate and is provided with a water absorbing membrane;

[0026] A first channel is arranged radially along the first partition group and is used to connect the reaction zone and the mixing zone;

[0027] A second channel is arranged radially along the first partition group and is used to connect the waste liquid area and the mixing area;

[0028] A cover plate is rotatably connected to the bottom plate, the cover plate forms a feed port corresponding to the mixing zone, and vertically protrudes from the lower surface to form a blocking member group, which can rotate in the movable channel to connect or block the first channel and the second channel;

[0029] The top cover may be connected to the cover plate and used to drive the cover plate to rotate circumferentially;

[0030] Mechanical seat, used to place the chassis;

[0031] A magnetic stirring device, comprising a magneton that can be placed in the mixing zone, and a control unit for controlling the rotation speed of the magneton;

[0032] The optical detection device is used to read the signal of the residue on the reaction membrane and output the detection result.

[0033] Preferably, the magnetic stirring device further comprises a sleeve arranged outside the magnet and a capillary connected to the sleeve.

[0034] The present invention further discloses a biological fluid detection method, comprising the following steps:

[0035] 1) Preparation, at this time, the first channel and the second channel are both in a blocked state;

[0036] 2) Sampling: aspirate the sample to be tested and drip it into the reaction reagent in the mixing zone through the feed port;

[0037] 3) Mixing: the magnetic particle rotates to mix the sample to be tested and the reaction reagent evenly;

[0038] 4) Reaction, the cover plate rotates to the first channel to connect the reaction zone and the mixing zone, and the magnet continues to rotate to diffuse the mixed solution to the reaction membrane for reaction, and the second channel is blocked at this time;

[0039] 5) Elution, the magnetic element stops rotating, and the cover plate is rotated to connect the waste liquid area and the mixing area through the second channel, and the unreacted reagent enters the waste liquid area;

[0040] 6) Detection: start the optical detection device and face the side wall where the reaction film is located to detect the residue on the reaction film and output the detection result.

[0041] Preferably, in step 5), the magnetic particle stops rotating, and the cover plate is rotated to the third channel to connect the liquid storage area and the mixing area, and the eluent sealed in the liquid storage area enters the mixing area. At this time, the first channel and the second channel are blocked; the cover plate is rotated to the first channel to connect the reaction area and the mixing area, so that the eluent diffuses to the reaction membrane to separate the unreacted reagent on the reaction membrane; the cover plate is rotated to the second channel to connect the waste liquid area and the mixing area, and the magnetic particle continues to rotate, and the eluent diffuses to the water absorption membrane.

[0042] The beneficial effects of the present invention are: 1) in the initial state, the mixing zone, reaction zone, liquid storage zone and waste liquid zone are independent of each other, and the reaction zone, liquid storage zone and waste liquid zone are relatively closed and will not be contaminated by the outside world, thereby increasing the shelf life of the detection device; 2) the mixing zone is controlled to be separately connected with the reaction zone, liquid storage zone and waste liquid zone through the circumferential rotation of the cover plate, and the control of the entire detection process is simple and reasonable; 3) the reaction zone, waste liquid zone and liquid storage zone are separately arranged, so they can be separately packaged and stored, thereby reducing the packaging, storage and transportation costs of the overall detection device; 4) the overall structure is simple and effective, and the production cost is relatively low. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a schematic diagram of the decomposition structure of the present invention.

[0044] Figure 2 It is a schematic diagram of the coordination structure of the chassis (without ribs) and the reaction zone, waste liquid zone and liquid storage zone of the present invention.

[0045] Figure 3 It is a schematic diagram of the lower surface structure of the cover plate of the present invention.

[0046] Figure 4 It is a top view of the cover plate of the present invention after a part is cut away and matched with the bottom plate.

[0047] Figure 5 It is a simplified cross-sectional view of the present invention.

[0048] Figure 6 It is a three-dimensional diagram of the mechanical base of the present invention.

[0049] Figure 7 It is a partial structural schematic diagram of the magnetic stirring device of the present invention.

[0050] Figure 8 4) is a schematic structural diagram of operation step 4) of the detection method in an embodiment of the present invention.

[0051] Fig. 9 Schematic diagram of the structure of operation step 5) of the detection method in an embodiment of the present invention.

[0052] Fig.10 Schematic diagram of the structure of operation step 6) of the detection method in an embodiment of the present invention.

[0053] Fig.11 Schematic diagram of the structure of operation step 7) of the detection method in an embodiment of the present invention.

[0054] Fig.12 Schematic diagram of the structure of operation step 8) of the detection method in an embodiment of the present invention.

[0055] Fig.13 Schematic diagram of the structure of operation step 9) of the detection method in an embodiment of the present invention. DETAILED DESCRIPTION

[0056] In order to enable those skilled in the art to better understand the solutions of the present invention, the following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0057] like Figure 1-7 As shown, a biological fluid detection device includes a circular bottom plate 1, a reaction zone 2 arranged along the outer periphery of the bottom plate 1, a waste liquid zone 3 arranged along the outer periphery of the bottom plate 1, a first channel 4 for connecting the reaction zone 2 and the mixing zone 11, a second channel 5 for connecting the waste liquid zone 3 and the mixing zone 11, and a cover plate 6 that can cover the bottom plate 1 from top to bottom.

[0058] Of course, in other embodiments, a liquid storage area 7 is also provided to accommodate detection requiring an eluent, and the liquid storage area 7 is provided along the periphery of the bottom plate 1 , and a third channel 8 is formed between the liquid storage area 7 and the mixing area 11 for connecting the two areas.

[0059] The chassis 1 has a circular flat plate structure, which includes a first partition group 12 and a second partition group 13 formed by vertically protruding upward on the upper surface, a mixing area 11 located in the center, and a movable channel 14.

[0060] The first partition group 12 includes a partition 1 121, a partition 2 122, and a partition 3 123 arranged at intervals along the circumferential direction. The partition 1 121, the partition 2 122, and the partition 3 123 are all arc-shaped sheet structures vertically fixedly connected to the upper surface of the chassis 1, and the three are concentrically arranged and located on the same circumference. Specifically, in this embodiment, the center angle of the interval between the partition 1 121 and the partition 2 122 is 30°, the center angle of the interval between the partition 2 122 and the partition 3 123 is 30°, the center angle of the interval between the partition 3 123 and the partition 1 121 is 30°, the center angle of the partition 1 121 is 60°, the center angle of the partition 2 122 is 90°, and the center angle of the partition 3 123 is 120°.

[0061] The second partition group 13 is arranged concentrically with the first partition group 12, and the second partition group 13 is located on the outer circle of the first partition group 12, and includes a partition 4 131 and a partition 5 132 arranged at intervals along the circumferential direction, and the partition 4 131 and the partition 5 132 are both arc-shaped sheet structures vertically fixedly connected to the upper surface of the chassis 1, and the two are arranged concentrically and located on the same circumference. Specifically, in this embodiment, the partition 5 132 is opposite to the partition 2 122, and the central angle thereof is about 90°, and the partition 4 131 is opposite to the partition 1 121 and the partition 3 123, and the central angle thereof is about 210°, so that two spacing gaps are formed between the partition 4 131 and the partition 5 132.

[0062] A waste liquid tank 31 is formed on one of the gaps between the partition 4 131 and the partition 5 132, and includes an arc surface 311 protruding from the circle where the partition 4 131 is located, a side 1 312 for connecting the arc surface 311 and the partition 4 131, and a side 2 313 for connecting the arc surface 311 and the partition 5 132. The waste liquid area 3 can be detachably connected to the waste liquid tank 31. The waste liquid area 3 can be a closed cavity with a shape and size that matches the waste liquid tank 31, which is tightly mounted in the waste liquid tank 31, and a water absorbing film 32 is connected to the side facing the center of the chassis 1.

[0063] A liquid storage tank 72 is formed on another gap between the partition 4 131 and the partition 5 132. The structure of the liquid storage tank 72 is the same as that of the waste liquid tank 31, and also includes an arc surface protruding from the circle where the partition 4 131 is located, which is used to connect the arc surface and the side 1 of the partition 4 131, and the side 2 of the partition 5 132. The liquid storage area 7 can be detachably connected to the liquid storage tank 72. The liquid storage area 7 can be a closed cavity that matches the shape and size of the liquid storage tank 72, such as Figure 5 As shown, its bottom surface is an inclined surface 71, and extends downward from the outer periphery of the bottom plate 1 toward the center, so that the eluent sealed in the liquid storage area 7 can flow along the inclined surface 71 toward the mixing area 11 under the action of gravity. Matchingly, the bottom surface of the liquid storage tank 72 also forms an inclined bottom surface with the same slope as the inclined surface 71.

[0064] Of course, the liquid storage tank 72 structure may also be omitted, and the liquid storage area 7 may be detachably connected to the area on the chassis 1 corresponding to the liquid storage tank 72 .

[0065] The side of the liquid storage area 7 facing the mixing area 11 is sealed with aluminum foil or the like, and a tip 614 that can cut the aluminum foil is provided on the outer wall of the third barrier 613. During the rotation of the third barrier 613, the tip 614 cuts the aluminum foil to release the eluent in the liquid storage area 7.

[0066] In order to strengthen the structure of the chassis 1, a first guard 151 and a second guard 152 are connected between the waste liquid tank 31 and the liquid storage tank 72. The first guard 151 is an arc sheet structure that is concentrically arranged opposite to the partition five 132, and is vertically connected to the upper surface of the chassis 1, and its two ends are respectively fixedly connected to the side surfaces of the waste liquid tank 31 and the liquid storage tank 72; the second guard 152 is an arc sheet structure that is concentrically arranged opposite to the partition four 131, and is vertically connected to the upper surface of the chassis 1, and its two ends are respectively fixedly connected to the side surfaces of the waste liquid tank 31 and the liquid storage tank 72.

[0067] The interval between the partition 1 121 and the partition 2 122 forms a second channel 5 for connecting the waste liquid area 3 and the mixing area 11 , that is, the second channel 5 is radially arranged along the first partition group 12 and passes through the first partition group 12 and the second partition group 13 .

[0068] The interval between the second partition 122 and the third partition 123 forms a third channel 8 for connecting the liquid storage area 7 and the mixing area 11 , that is, the third channel 8 is arranged radially along the first partition group 12 and passes through the first partition group 12 and the second partition group 13 .

[0069] The gap between partition one 121 and partition three 123 forms a first channel 4. In the initial state, the reaction zone 2 is stuck between the gap and the inner wall of the second partition group 13, that is, the first channel 4 is radially arranged along the first partition group 12, and passes through the first partition group 12 to connect the reaction zone 2 and the mixing zone 11.

[0070] The width of the reaction zone 2 is adapted to the radial spacing between the first partition group 12 and the second partition group 13, and the circumferential arc of the reaction zone 2 is adapted to the spacing between the partition 1 121 and the partition 3 123, and is slightly longer than the arc of the spacing. The reaction zone 2 is a closed cavity, and a reaction membrane 21 is connected to its side wall facing the center of the chassis 1; in order to facilitate the removal and placement of the reaction zone 2, a ball handle 22 is connected to its top surface.

[0071] The movable channel 14 includes an annular space between the outer wall of the first partition group 12 and the inner wall of the second partition group 13 , and may also include a partial space close to the inner wall of the first partition group 12 .

[0072] like Figure 3 As shown, the main body of the cover plate 6 is a circular flat plate structure that is adapted to the size and shape of the main body of the chassis 1. The center of the structure corresponds to the position of the mixing zone 11 to form a circular feed port 63, and the lower surface of the structure vertically protrudes to form a barrier group 61. When the chassis 1 and the cover plate 6 are assembled up and down, the barrier group 61 extends downward into the movable channel 14 and rotates.

[0073] Specifically, the barrier group 61 includes barrier 1 611, barrier 2 612 and barrier 3 613 which are concentrically and spaced apart. Barrier 2 612 and barrier 3 613 are located on the same circumference, barrier 1 611 is located on a circumference with a slightly smaller inner diameter, and the thickness of barrier 2 612 and barrier 3 613 are adapted to the width of the annular space between the outer wall of the first partition group 12 and the inner wall of the second partition group 13, and the thickness of barrier 1 611 is smaller.

[0074] The first blocking element 611 is an arc-shaped sheet structure, and its central angle is about 90°, that is, Figure 4 The middle angle α is 90°; the second blocking member 612 is an arc-shaped block structure, and its central angle is about 120°, that is, Figure 4 The middle β angle is 90°; the third blocking member 613 is an arc-shaped block structure, and its central angle is about 60°, that is, Figure 4 The median γ angle is 90°.

[0075] When the cover plate 6 and the bottom plate 1 are assembled together, and the cover plate 6 and the bottom plate 1 are rotatably sealed and connected, the feed port 63 faces the mixing zone 11, and the second barrier 612 and the third barrier 613 are inserted into the active channel 14 and located in the annular space between the outer wall of the first partition group 12 and the inner wall of the second partition group 13. When the cover plate 6 rotates, the second barrier 612 and the third barrier 613 can rotate circumferentially in the annular space to achieve the purpose of connecting or blocking the second channel 5 and the third channel 8; the barrier 1 611 is inserted into the active channel 14 and located in the inner wall part of the first partition group 12, that is, the side wall of the barrier 1 611 and the inner wall of the first partition group 12 rotate in fit, to achieve the purpose of connecting or blocking the first channel 4.

[0076] In order to ensure the stability and sealing of the rotational connection between the cover plate 6 and the chassis 1, the chassis 1 and the cover plate 6 can be made of waterproof materials with certain elasticity, such as rubber and elastic plastic.

[0077] In order to facilitate the extension of the ball handle 22 at the top of the reaction zone 2, an arc-shaped groove 64 is opened on the cover plate 6. In order to facilitate the connection between the cover plate 6 and the top cover 94, a positioning column 62 is set on the upper surface of the cover plate 6.

[0078] When the biological fluid detection device of the present invention is used to detect glycated hemoglobin, the reaction reagent in the mixing zone 11 includes a phenylboronic acid dye complex for lysing red blood cells, releasing glycated hemoglobin, and precipitating total hemoglobin, which can be a mixed solution composed of 50-300mmol / L of glycine, 10-40mmol / L of zinc chloride, 5-20mmol / L of magnesium chloride, 100-400mmol / L of sodium chloride, 0.05-0.5mmol / L of phenylboronic acid dye complex, 10-50% by volume of methanol, 1-5% by mass volume of polyvinyl pyrrolidone, and 0.1-1% by volume of polyethylene glycol octylphenyl ether, and the pH value is 8.5-9.5.

[0079] In order to improve the sealing reliability of the biological fluid detection device during transportation and minimize leakage caused by liquid reaction reagents, the reaction reagents in the mixing area 11 are freeze-dried during the production process to form a solid droplet or a similar shape, which is spotted at the center of the mixing area 11 and can be re-dissolved when used.

[0080] The eluent in the liquid storage area 7 can be a mixture of 50-200 mmol / L tris(hydroxymethyl)aminomethane, 1-5% by volume glycerol, 0.1-0.5% by volume Proclin300, and 0.05-0.2% by volume polyethylene glycol octylphenyl ether, with a pH of 7.5-8.5.

[0081] A biological fluid detection system includes the above-mentioned base plate 1, the above-mentioned reaction area 2, the above-mentioned waste liquid area 3, the above-mentioned first channel 4, the above-mentioned second channel 5, the above-mentioned cover plate 6, a mechanical seat 91 for placing a biological fluid detection device, a magnetic stirring device partially installed in the mechanical seat 91, a top cover 94 that can be connected to the cover plate 6 and is used to drive the cover plate 6 to rotate circumferentially, and an optical detection device 93.

[0082] A groove 911 is formed on the mechanical seat 91, which is adapted to the shape and size of the biological fluid detection system. The protruding arrangement of the liquid storage area 7 and the waste liquid area 3 also realizes the anti-rotation cooperation between the bottom plate 1 and the groove 911. The top cover 94 is formed with a limit groove 941 for the positioning column 62 to extend out, a driving shaft 942 that can be connected to an external power source, and an open groove on the bottom surface for the ball handle 22 on the top of the reaction area 2 to extend into (not shown in the figure). In order to control the rotation stroke of the cover plate 6, two positioning columns 62 are connected to the upper surface of the cover plate 6.

[0083] The magnetic stirring device includes a magnet 92 that can be placed in the mixing zone 11, a sleeve 921 that is sleeved outside the magnet 92, a capillary 922 connected to the sleeve 921, and a control unit (not shown in the figure) for controlling the rotation speed and rotation direction of the magnet 92, and the control unit is installed in the mechanical base 91; the sleeve 921 is wrapped around the outside of the magnet 92, the capillary 922 is connected to the sleeve 921, and one end of the capillary 922 protrudes from the sleeve 921 to facilitate blood collection, so that the blood collected in the capillary 922 can be completely mixed with the reagent, thereby achieving quantitative collection and quantitative reaction; the optical detection device 93 is equipped with a detection light source required for the reaction, which can be directly facing the area where the reaction membrane 21 of the reaction zone 2 is located, read the signal of the residue on the reaction membrane 21, and output the detection result.

[0084] like Figure 8-13 As shown, a biological fluid detection method comprises the following steps:

[0085] 1) Preparation: Place the biological fluid detection device on the mechanical base 91. At this time, the first channel 4 is blocked by the blocking member 1 611, the second channel 5 is blocked by the blocking member 2 612, and the third channel 8 is blocked by the blocking member 3 613;

[0086] 2) Take an appropriate amount of purified water, such as 100-1000ul, and add it to the mixing area 11 to re-dissolve the lyophilized reagent in the mixing area 11;

[0087] 3) Sampling: sucking the sample to be tested through the feed port 63 and placing it into the reaction reagent in the mixing area 11; or placing the magnetic particle 92 with the cover 921 and the sample to be tested sucked by the capillary 922 into the mixing area 11;

[0088] 4) Mixing: start the magnetic stirring device, and the magnetic element 92 is driven by the electromagnetic force to start rotating in the mixing area 11 to mix the sample to be tested and the reaction reagent evenly;

[0089] 5) Reaction, such as Figure 4 and Fig. 9 As shown, the cover plate 6 rotates counterclockwise by 30°, the blocking element 1 611 releases the blocking of the first channel 4, and the magnetic element 92 stirs so that the reaction reagent diffuses onto the reaction membrane 21 of the reaction zone 2. At this time, the second channel 5 remains blocked by the blocking element 2 612, and the third channel 8 remains blocked by the blocking element 3 613;

[0090] 6) Elution, such as Fig.10 As shown, the magnetic element 92 stops stirring, the cover plate 6 rotates 60° clockwise, the blocking element 3 613 releases the blocking of the third channel 8, and the eluent in the liquid storage area 7 diffuses to the mixing area 11 under the action of gravity. At this time, the second channel 5 remains blocked by the blocking element 2 612, and the first channel 4 is blocked by the blocking element 1 611. The magnetic element 92 continues stirring to achieve a better cleaning effect.

[0091] 7) If Fig.11 As shown, the cover plate 6 rotates counterclockwise by 60°, the blocking element 1 611 releases the blocking of the first channel 4, the magnetic element 92 continues to stir, and the eluent diffuses onto the reaction membrane 21 of the reaction zone 2, so that the eluent cleans the unbound reagent on the reaction membrane. At this time, the second channel 5 remains blocked by the blocking element 2 612, and the third channel 8 remains blocked by the blocking element 3 613;

[0092] 8) If Fig.12 As shown, the cover plate 6 rotates 90° clockwise, the second blocking element 612 releases the blocking of the second channel 5, the magnetic element 92 continues to stir, and the excess eluent and unreacted reagents pass through the water absorption membrane 32 under the action of centrifugal force and are collected in the waste liquid area 3;

[0093] 9) Detection, such as Fig.13As shown, the biological fluid detection device is taken out from the mechanical seat 91, or the area where the reaction membrane 21 is located is placed facing the optical detection device 93 through the setting of the internal mechanical structure, and the optical detection device 93 is used to read the signal of the residue on the reaction membrane 21 and output the detection result.

[0094] The above specific implementation modes are used to explain the present invention rather than to limit the present invention. Any modification and change made to the present invention within the spirit of the present invention and the protection scope of the claims shall fall within the protection scope of the present invention.

Claims

1. A biological fluid detection device, characterized in that include: The chassis (1) comprises a mixing area (11) formed in the center, a first partition group (12) and a second partition group (13) vertically protruding from the upper surface and arranged concentrically, and a movable channel (14); the second partition group (13) is located on the outer circle of the first partition group (12), and the movable channel (14) is formed between the first partition group (12) and the second partition group (13); A reaction zone (2) is arranged along the outer periphery of the bottom plate (1) and is provided with a reaction membrane (21); A waste liquid area (3) is arranged along the outer periphery of the bottom plate (1) and is provided with a water absorbing membrane (32); A first channel (4) is arranged radially along the first partition group (12) and is used to connect the reaction zone (2) and the mixing zone (11); A second channel (5) is arranged radially along the first partition group (12) and is used to connect the waste liquid area (3) and the mixing area (11); The cover plate (6) is rotatably sealed and connected to the bottom plate (1). The cover plate (6) forms a feed inlet (63) corresponding to the mixing zone (11). A blocking member group (61) is vertically protruded from the lower surface. The blocking member group (61) can rotate in the movable channel (14) to connect or block the first channel (4) and the second channel (5).

2. The biological fluid detection device according to claim 1, characterized in that: It also includes a liquid storage area (7) arranged on the periphery of the bottom plate (1), and a third channel (8) for connecting the liquid storage area (7) and the mixing area (11).

3. The biological fluid detection device according to claim 2, characterized in that: The blocking member group (61) comprises a blocking member 1 (611), a blocking member 2 (612) and a blocking member 3 (613) which are concentrically and spaced apart from each other. The blocking member 1 (611), the blocking member 2 (612) and the blocking member 3 (613) can rotate in the movable channel (14) to connect or block the first channel (4), the second channel (5) and the third channel (8).

4. The biological fluid detection device according to claim 2, characterized in that: The movable channel (14) comprises an annular space between the outer wall of the first partition group (12) and the inner wall of the second partition group (13), and a partial space close to the inner wall of the first partition group (12); the second blocking member (612) and the third blocking member (613) can rotate in the annular space to connect or block the second channel (5) and the third channel (8); the first blocking member (611) can rotate in contact with the inner wall of the first partition group (12) to connect or block the first channel (4).

5. The biological fluid detection device according to claim 2, characterized in that: The bottom surface of the liquid storage area (7) is provided with an inclined surface (71) for facilitating the eluent in the liquid storage area (7) to flow toward the mixing area (11); the first partition group (12) comprises a partition one (121), a partition two (122) and a partition three (123) which are arranged concentrically and spaced apart in the circumferential direction; and the second partition group (13) comprises a partition four (131) and a partition five (132) which are arranged concentrically and spaced apart in the circumferential direction.

6. The biological fluid detection device according to claim 5, characterized in that: The outer periphery of the chassis (1) is formed with a waste liquid tank (31) and a liquid storage tank (72) arranged at intervals; the waste liquid area (3) is detachably connected to the waste liquid tank (31); the liquid storage area (7) is detachably connected to the liquid storage tank (72); the reaction area (2) is arranged between the first partition group (12) and the second partition group (13); the waste liquid tank (31) is arranged between partition four (131) and partition five (132); the liquid storage tank (72) is arranged between partition four (131) and partition five (132); and the cover plate (6) is provided with a positioning column (62) that can be connected to an external power source.

7. A biological fluid detection system, characterized in that include: The chassis (1) comprises a mixing area (11) formed in the center, a first partition group (12) and a second partition group (13) vertically protruding from the upper surface and arranged concentrically, and a movable channel (14); the second partition group (13) is located on the outer circle of the first partition group (12), and the movable channel (14) is formed between the first partition group (12) and the second partition group (13); A reaction zone (2) is arranged along the outer periphery of the bottom plate (1) and is provided with a reaction membrane (21); A waste liquid area (3) is arranged along the outer periphery of the bottom plate (1) and is provided with a water absorbing membrane (32); A first channel (4) is arranged radially along the first partition group (12) and is used to connect the reaction zone (2) and the mixing zone (11); A second channel (5) is arranged radially along the first partition group (12) and is used to connect the waste liquid area (3) and the mixing area (11); A cover plate (6) is rotatably connected to the bottom plate (1), the cover plate (6) forms a feed port (63) corresponding to the mixing zone (11), and a blocking member group (61) is vertically protruded from the lower surface. The blocking member group (61) can rotate in the movable channel (14) to connect or block the first channel (4) and the second channel (5); A top cover (94) may be connected to the cover plate (6) and is used to drive the cover plate (6) to rotate in a circumferential direction; A mechanical seat (91) for placing the chassis (1); A magnetic stirring device, comprising a magnet (92) that can be placed in a mixing zone (11), and a control unit for controlling the rotation speed of the magnet (92); The optical detection device (93) is used to read the signal of the residue on the reaction film (21) and output the detection result.

8. The biological fluid detection system according to claim 7, characterized in that: The magnetic stirring device further comprises a sleeve (921) sleeved outside the magnet (92), and a capillary (922) connected to the sleeve (921).

9. A biological fluid detection method, implemented by the biological fluid detection device according to any one of claims 1 to 6, or implemented by the biological fluid detection system according to claim 7, characterized in that The following steps are involved: 1) Preparation, at this time, the first channel and the second channel are both in the blocked state; 2) Sampling: the sample to be tested is drawn through the feed port and dripped into the reaction reagent in the mixing zone; 3) Mixing: The magnetic particles rotate to mix the sample and the reaction reagents evenly; 4) Reaction, the cover plate rotates to the first channel to connect the reaction zone and the mixing zone, and the magnet continues to rotate to diffuse the mixed solution to the reaction membrane for reaction, and the second channel is blocked at this time; 5) Elution, the magnetic element stops rotating, and the cover plate is rotated to the second channel to connect the waste liquid area and the mixing area, and the unreacted reagent enters the waste liquid area; 6) Detection: Start the optical detection device and face the side wall where the reaction film is located to detect the residue on the reaction film and output the detection result.

10. The biological fluid detection method according to claim 9, characterized in that: In the step 5), the magnetic particle stops rotating, and the cover plate is rotated to the third channel to connect the liquid storage area and the mixing area, and the eluent sealed in the liquid storage area enters the mixing area. At this time, the first channel and the second channel are blocked; the cover plate is rotated to the first channel to connect the reaction area and the mixing area, so that the eluent diffuses to the reaction membrane to separate the unreacted reagent on the reaction membrane; the cover plate is rotated to the second channel to connect the waste liquid area and the mixing area, and the magnetic particle continues to rotate, and the eluent diffuses to the water absorption membrane.

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