Portable biochemical reactor

Abstract

A portable biochemical reactor is applicable to driving at least one stirring sleeve to displace relative to at least one reagent cartridge along a first axial direction, and includes a frame unit and a variable-speed lifting unit. The frame unit is applicable to supporting the at least one reagent cartridge. The variable-speed lifting unit includes a linear movement module, a variable-speed transmission module, and a rotation module. The linear movement module has a slider applicable to installing the at least one stirring sleeve. The slider moves between a top position and a bottom position relative to the frame unit. When at the top position, the slider is applicable to moving away from the at least one reagent cartridge. When at the bottom position, the slider is applicable to approaching the at least one reagent cartridge. The variable-speed transmission module has a transmission member and a guiding member. The rotation module is used for driving the transmission member to rotate, thereby driving the slider to displace along the first axial direction following the guide rail, and synchronously driving the at least one stirring sleeve to perform a variable-speed linear movement.

Description

Technical Field

[0001] The present invention relates to a biochemical reactor, and particularly to a portable biochemical reactor. Background Art

[0002] Existing biochemical reactors for nucleic acid extraction using silica magnetic beads are aimed at extracting nucleic acids (DNA or RNA) from biological samples and further providing them for use in biological detections such as polymerase chain reaction (PCR).

[0003] When the biochemical reaction is carried out, in order to improve the extraction rate of nucleic acids, the biological sample and the magnetic beads are repeatedly and fully mixed with reagents with different functions by stirring, and biochemical reaction procedures such as lysis, washing, and nucleic acid recovery are carried out in sequence, and finally purified nucleic acids are extracted.

[0004] The above-mentioned stirring method usually uses a form of a motor with a screw rod, and stirs in the reagent in a linear motion with equal speed up and down. In order to achieve better stirring uniformity, a relatively expensive controller and complex electronic control programming are often required to achieve the purpose of stirring. In addition, since the motor needs to rotate several circles to drive the screw rod to reach a certain moving stroke, the existing biochemical reactor has a large power consumption. Moreover, the form of the motor with the screw rod will also make the overall equipment have a certain weight, making it difficult to be lightweight and not conducive to carrying.

[0005] Such disadvantages will make it difficult for the existing biochemical reactor to be used in the first-line occasions outside the laboratory, such as the quarantine site of the aquaculture industry or the forensic site... etc. And due to the relatively high cost of the overall structure, it is also difficult to popularize, and there is great room for improvement in use. Summary of the Invention

[0006] The purpose of the present invention is to provide a portable biochemical reactor that can improve stirring efficiency, reduce costs, and is conducive to carrying.

[0007] The portable biochemical reactor of the present invention is suitable for driving at least one stirring sleeve to displace relative to at least one reagent cartridge along a first axis, and the portable biochemical reactor includes a frame unit and at least one variable-speed lifting unit.

[0008] The frame unit is suitable for supporting the at least one reagent cartridge.

[0009] The at least one variable-speed lifting unit includes a linear movement module, a variable-speed transmission module, and a rotation module. The linear movement module has a guide rail connected to the frame unit, and a slider movably connected to the guide rail along the first axial direction and adapted to mount the at least one stirring sleeve. The slider moves relative to the frame unit between a top position and a bottom position. At the top position, the slider is adapted to be away from the at least one reagent cartridge. At the bottom position, the slider is adapted to be adjacent to the at least one reagent cartridge. The variable-speed transmission module has a transmission member and a guiding member. The transmission member has a pivot portion and a guiding portion arranged in reverse. The guiding member is movably connected to the guiding portion. One of the guiding member and the pivot portion of the transmission member is connected to the slider. The rotation module is connected to the other of the guiding member and the pivot portion of the transmission member and is used to drive the transmission member to rotate. During the rotation of the transmission member, the slider is driven by the guiding member to displace along the first axial direction along the guide rail, and the at least one stirring sleeve is synchronously driven to perform a variable-speed linear movement.

[0010] In the portable biochemical reactor of the present invention, the at least one reagent cartridge is filled with reagents and a liquid level is defined. The slider can also move relative to the frame unit to a midpoint position between the top position and the bottom position. At the midpoint position, the slider is adapted to drive the bottom end of the at least one stirring sleeve to be adjacent to the liquid level, and the instantaneous speed of the slider moving to the midpoint position is slower than the instantaneous speed when moving to be adjacent to the top position.

[0011] In the portable biochemical reactor of the present invention, a second axial direction and a third axial direction orthogonal to the first axial direction and orthogonal to each other are defined. The rotation module is located on two opposite sides of the guide rail along the third axial direction with respect to the guiding member, and has a power member and an output shaft. The power member is connected to the frame unit. The output shaft is pivotally arranged on the power member along the second axial direction and is connected to the other of the guiding member and the pivot portion of the transmission member with respect to the linear movement module.

[0012] In the portable biochemical reactor of the present invention, the slider is connected to the guiding member, and the pivot portion of the transmission member is fixed to the rotation module.

[0013] In the portable biochemical reactor of the present invention, the guiding portion of the transmission member is a long slot, and the guiding member slides within the guiding portion.

[0014] The portable biochemical reactor of the present invention further includes a reagent cassette transfer unit, and a second axis orthogonal to the first axis is defined. The reagent cassette transfer unit includes two transfer tracks disposed on the frame unit along the second axis, a transfer seat movably disposed on the transfer tracks, and a driving module for driving the transfer seat to move relative to the frame unit.

[0015] For the portable biochemical reactor of the present invention, the driving module has a rack mounted on the transfer seat, a gear pivotally mounted on the frame unit, and a driving member for driving the gear.

[0016] For the portable biochemical reactor of the present invention, the frame unit includes a bottom frame and a side frame. The side frame is disposed on the bottom frame and has an opening. The transfer seat of the reagent cassette transfer unit has a seat body portion disposed on the transfer tracks, and a jig portion removably connected to the seat body portion. The jig portion is used for mounting the at least one reagent cassette and can be taken out and put into the opening of the side frame.

[0017] For the portable biochemical reactor of the present invention, the reagent cassette transfer unit further includes a heating module. The transfer seat has a seat body portion and a jig portion. The seat body portion is disposed on the transfer tracks and is for mounting the heating module. The jig portion is removably connected to the seat body portion and is used for mounting the at least one reagent cassette and has through holes corresponding to the heating module.

[0018] The portable biochemical reactor of the present invention further includes a magnetic bead transfer unit and two variable-speed lifting units. The at least one reagent cassette is suitable for loading several magnetic beads. The slider of one variable-speed lifting unit is connected to the stirring sleeve, and the other variable-speed lifting unit is connected to the magnetic bead transfer unit. The magnetic bead transfer unit includes at least one magnetic rod inserted into the at least one stirring sleeve. The at least one magnetic rod has a magnetic portion adjacent to the bottom of the stirring sleeve. When the slider is in the bottom position, the magnetic portion is suitable for magnetically adsorbing the magnetic beads on the stirring sleeve.

[0019] The beneficial effects of the present invention are as follows: By means of the variable-speed transmission module, the at least one stirring rod can be driven to perform variable-speed linear movement, thereby improving the stirring efficiency, reducing the cost, and reducing the volume and weight, achieving the purpose of being convenient to carry. Description of the Drawings

[0020] Figure 1 is an exploded perspective view showing the positional relationship between an embodiment of the portable biochemical reactor of the present invention, a stirring row member, and four reagent cassettes;

[0021] Figure 2 is a perspective view showing the embodiment;

[0022] Figure 3 is a schematic diagram illustrating this embodiment;

[0023] Figure 4 is an incomplete partial cross-sectional view illustrating the positional relationship between four stirring sleeves and the reagent cassette when a slider is in a top position in this embodiment;

[0024] Figure 5 is similar to Figure 4 and is an incomplete partial cross-sectional view illustrating the positional relationship between the stirring sleeve and the reagent cassette when the slider is in a midpoint position;

[0025] Figure 6 is similar to Figure 4 and is an incomplete partial cross-sectional view illustrating the positional relationship between the stirring sleeve and the reagent cassette when the slider is in a bottom position;

[0026] Figure 7 is an incomplete side view illustrating a variation of this embodiment;

[0027] Figure 8 is an incomplete partial exploded perspective view illustrating the positional relationship between the stirring row member and a row member fixing unit in this embodiment;

[0028] Figure 9 is similar to Figure 6 and is an incomplete partial cross-sectional view illustrating the positional relationship between four magnetic rods and the stirring sleeve when another slider is in a bottom position. Detailed Description of the Embodiment

[0029] The present invention will be described in detail below with reference to the accompanying drawings and embodiments.

[0030] Refer to Figure 1 , Figure 2 , and Figure 3 . In an embodiment of the portable biochemical reactor of the present invention, a first axis L1, a second axis L2, and a third axis L3 that are orthogonally arranged to each other are defined, and it is applicable to drive a stirring row member 22 to displace relative to four reagent cassettes 21 along the first axis L1.

[0031] The reagent cassettes 21 are arranged at intervals along the third axis L3. Each reagent cassette 21 includes a plurality of reagent grooves 211 arranged along the second axis L2 and used for loading reagents 213, and a plurality of magnetic beads 212 accommodated in one of the reagent grooves 211. The reagent 213 in each reagent groove 211 defines a liquid level 214.

[0032] The stirring member 22 has four stirring sleeves 221 arranged at intervals along the third axial direction L3. Each stirring sleeve 221 can be inserted into a respective reagent tank 211, and can be immersed in the reagent 213 by moving away from the liquid surface 214 along the first axial direction L1 or passing through the liquid surface 214.

[0033] It should be noted that the number of the reagent cartridges 21 and the stirring sleeves 221 is not limited to four, and can also be less than three or more than five. And in other variations of this embodiment, the reagent cartridge 21 can also be replaced with a deep well plate.

[0034] This embodiment includes a frame unit 3, a reagent cartridge transfer unit 4, two variable-speed lifting units 5, a member fixing unit 6, a magnetic bead transfer unit 7, and a disinfection unit 8.

[0035] The frame unit 3 is suitable for supporting the reagent cartridge 21, and includes a base frame 31, two side frames 32, and two extension brackets 33.

[0036] The side frames 32 are arranged at intervals along the third axial direction L3 on the base frame 31, and each side frame 32 has an opening 321. In other variations of this embodiment, it can also be that only one of the side frames 32 has the opening 321, which is not limited thereto.

[0037] The extension brackets 33 are connected between the side frames 32 along the third axial direction L3, and are arranged at intervals along the second axial direction L2.

[0038] In this embodiment, the side frames 32 are arranged on two opposite sides of the base frame 31 along the third axial direction L3. However, in other variations of this embodiment, the frame unit 3 can also only include one side frame 32, and at this time the extension brackets 33 are connected to the side frame 32. In another variation, the side frames 32 can also be arranged across the base frame 31 along the second axial direction L2, which is not limited thereto.

[0039] The reagent cartridge transfer unit 4 is arranged on the frame unit 3, and includes two transfer tracks 41, a transfer seat 42, a drive module 43, and a heating module 44.

[0040] The transfer tracks 41 are arranged on the base frame 31 at a distance and along the second axial direction L2.

[0041] The transfer base 42 has a base body 421, a jig part 422, and a jig sensor 423. The base body 421 is movably disposed between the transfer tracks 41. The jig part 422 is removably disposed on the base body 421 and is adapted to mount the reagent cartridge 21, and has a through hole 424 communicating with the base body 421. The jig sensor 423 is mounted on the base body 421 for sensing whether the jig part 422 is located on the base body 421.

[0042] In this embodiment, the jig part 422 can be taken out and put into the opening 321 of the corresponding side frame 32. However, in other variations, the jig part 422 can also be taken out and put into the base body 421 along the second axis L2. At this time, the side frames 32 may not have the opening 321, and this is not limited thereto.

[0043] The driving module 43 is used to drive the transfer base 42 and move the transfer base 42 relative to the frame unit 3, and has a rack 431 mounted on the transfer base 42, a gear 432 pivotally mounted on the frame unit 3, and a driving member 433 for driving the gear 432. When the driving member 433 drives the gear 432 to rotate, the transfer base 42 is driven by the rack 431, and the transfer base 42 is transferred along the second axis L2 on the transfer track 41, whereby the stirring sleeve 221 can be correspondingly aligned with the reagent grooves 211 of the reagent cartridge 21 in sequence. It should be noted that in other variations of this embodiment, a pulley and belt form can also be used to achieve the driving purpose, but this is not limited thereto.

[0044] The heating module 44 is disposed on the base body 421 of the transfer base 42 and corresponds to the through hole 424 of the jig part 422. When the operator mounts the reagent cartridge 21 on the jig part 422, one of the reagent grooves 211 of each reagent cartridge 21 is adjacent to and corresponds to the heating module 44, and the reagent is heated by the heating module 44, thereby improving the efficiency of a specific biochemical reaction. In this embodiment, the heating module 44 is a metal heating sheet, but this is not limited thereto.

[0045] Each speed-changing lifting unit 5 is connected to the frame unit 3 and includes a linear movement module 51, a speed-changing transmission module 52, and a rotation module 53.

[0046] The linear movement module 51 is disposed on one of the extension brackets 33 of the frame unit 3 and has a guide rail 511 connected to the one extension bracket 33, and a slider 512 movably connected to the guide rail 511 along the first axis L1 and adapted to mount the stirring row member 22.

[0047] The slider 512 is at a top position relative to the frame unit 3 (such asFigure 4 as shown), a midpoint position (such as Figure 5 as shown), and a bottom position (such as Figure 6 as shown). The midpoint position is located between the top position and the bottom position.

[0048] When at the top position, the slider 512 is adapted to move away from the reagent cassette 21, and simultaneously drive the bottom end of the stirring sleeve 221 away from the reagent cassette 21.

[0049] When at the midpoint position, the slider 512 is adapted to drive the bottom end of the stirring sleeve 221 adjacent to the liquid level 214.

[0050] When at the bottom position, the slider 512 is adapted to be adjacent to the reagent cassette 21, and simultaneously drive the stirring sleeve 221 through the liquid level 214 and adjacent to the bottom end of the reagent cassette 21.

[0051] The variable speed transmission module 52 is connected between the linear movement module 51 and the rotation module 53, and has a transmission member 521 and a guide member 522. The transmission member 521 has a pivot portion 523 and a guide portion 524 which are arranged in reverse. The guide member 522 is movably connected to the guide portion 524. In this embodiment, the guide portion 524 of the transmission member 521 is a long hole, and the guide member 522 is in the shape of a round rod and is rotatably slidable in the guide portion 524, but is not limited thereto.

[0052] The rotation module 53 has a power member 531 and an output shaft 532 driven by the power member 531. The power member 531 is connected to one of the side frames 32 of the frame unit 3. The output shaft 532 is pivotally provided on the power member 531 along the second axial direction L2, and can rotate bidirectionally, and is connected to the variable speed transmission module 52. In this embodiment, the output shaft 532 is connected to the pivot portion 523 of the transmission member 521. At this time, the guide member 522 is connected to the slider 512 of the linear movement module 51.

[0053] When the output shaft 532 drives the transmission member 521 to rotate, it will simultaneously cause the guide portion 524 of the transmission member 521 to move and rotate relative to the guide member 522, thereby driving the slider 512 to displace along the first axial direction L1 along the guide rail 511, and simultaneously driving the stirring sleeve 221 to perform variable speed linear movement.

[0054] It should be noted that, in this embodiment, the rotation module 53 is a stepping motor, and is located on two opposite sides of the guide member 522 along the third axial direction L3 with respect to the guide rail 511. By virtue of the characteristics of the stepping motor such as low cost, high torque, and open-loop precise positioning control, it is beneficial to cooperate with the variable speed transmission module 52 to achieve the advantage of stable variable speed movement.

[0055] Refer to Figure 7 , in other variations of this embodiment, it is also possible to pivotally connect the pivoting portion 523' to the slider 512'. At this time, the guiding member 522' is fixed to the frame unit 3', the output shaft 532' is cam-shaped and is used to push against the guiding portion 524' of the transmission member 521', and the guiding portion 524' of the transmission member 521' rotates and moves relative to the guiding member 522'. This is not the only limit.

[0056] Refer to Figure 1 、 Figure 3 , and Figure 8 , the discharge member fixing unit 6 is disposed on the linear movement module 51 of one of the variable speed lifting units 5, and includes a discharge member fixing seat 61 disposed on the slider 512 and suitable for mounting the stirring discharge member 22, and a discharge member sensor 62.

[0057] In this embodiment, the stirring discharge member 22 is taken out from and put into the opening 321 of the corresponding side frame 32, and the discharge member sensor 62 is used to sense whether the stirring discharge member 22 is actually mounted on the discharge member fixing seat 61.

[0058] It is worth noting that in other variations of this embodiment, the stirring sleeves 221 can also be separately provided and respectively mounted on the discharge member fixing seat 61. At this time, each stirring sleeve 221 is first accommodated in one of the reagent grooves 211 of its respective reagent cartridge 21. The discharge member fixing seat 61 is suitable for connecting and fixing the respective stirring sleeves 221 from the corresponding reagent grooves 211 along the first axial direction L1. This is not the only limit.

[0059] Refer to Figure 1 、 Figure 3 , and Figure 9 , the magnetic bead transfer unit 7 is disposed on the linear movement module 51 of the other variable speed lifting unit 5 relative to the discharge member fixing unit 6, and includes a magnetic rod holder 71 connected to the corresponding slider 512, and four magnetic rods 72 corresponding to the stirring sleeves 221.

[0060] In this embodiment, the magnetic rod holder 71 and the corresponding slider 512 are integrally formed. However, in other variations, they can also be separately manufactured and then assembled and combined. This is not the only limit.

[0061] Each magnetic rod 72 is inserted into its respective stirring sleeve 221, and has a rod portion 721 connecting the magnetic rod holder 71 and a magnetic portion 722 adjacent to the bottom of the stirring sleeve 221. When the sliders 512 of the variable speed lifting unit 5 are all located at the bottom position, the magnetic portion 722 is suitable for magnetically adsorbing the magnetic beads 212 on the stirring sleeve 221 (as shown in Figure 9 ).

[0062] The disinfection unit 8 includes a UV lamp 81 disposed on the frame unit 3. In this embodiment, the UV lamp 81 is adjacent to the reagent cassette transfer unit 4 and the magnetic bead transfer unit 7.

[0063] With the above structure, the operation procedure of this embodiment when applied to the biochemical reaction of nucleic acid extraction is described as follows:

[0064] Step 1: The operator prepares the unused reagent cassette 21 and the stirring row member 22, and places several biological samples S to be tested in one of the reagent slots 211 of different reagent cassettes 21 correspondingly.

[0065] Step 2: One of the variable speed lifting units 5 drives the row member fixing unit 6 to descend, and the operator fixes the stirring row member 22 to the row member fixing seat 61. Then, the one variable speed lifting unit 5 drives the row member fixing unit 6 to rise and move away from the chassis 31. The operator first fixes the reagent cassette 21 to the jig part 422 of the transfer seat 42, and then fixes the jig part 422 together with the reagent cassette 21 to the seat body part 421 of the transfer seat 42. At the same time, the jig sensor 423 of the transfer seat 42 and the row member sensor 62 of the row member fixing unit 6 will detect whether they are correctly installed, and then the subsequent biochemical reaction is carried out, thereby achieving a safe operation mechanism.

[0066] The following steps 3 to 6 illustrate how to perform reaction procedures such as lysis, washing, and nucleic acid recovery on the biological sample S in the reagent slot 211 in sequence, in combination with the magnetic beads 212 and reagents 213 of different properties.

[0067] Step 3: First, the cell wall and cell membrane of the biological sample S need to be separated and destroyed in the reagent 213 with the function of cell tissue lysis, in combination with the magnetic beads 212, to release the nucleic acid therein. In this embodiment, the one variable speed lifting unit 5 is used to drive the stirring sleeve 221 to perform short-distance up-and-down variable speed stirring in the reagent slot 211, thereby achieving the effects of homogenization and shortening the reaction time. It should be noted that during the stirring, the slider 512 of the other variable speed lifting unit 5 is continuously located at the top position and drives the magnetic rod 72 to move away from the stirring sleeve 221.

[0068] In addition, it is worth noting that in this embodiment, the following effects are also achieved: when the slider 512 of the linear movement module 51 is at the midpoint position (such as Figure 5When the bottom end of the stirring sleeve 221 is adjacent to the liquid surface 214 as shown, at this time, since the distance between the guiding member 522 and the output shaft 532 is reduced, the moving speed of the slider 512 will be slowed down, thereby avoiding the splashing of the reagent 213 or the biological sample S caused when the stirring sleeve 221 breaks through the liquid surface 214, and further reducing the generation of contamination. That is to say, the instantaneous speed of the slider 512 moving to the midpoint position (as shown in Figure 5 is slower than the instantaneous speed when moving to a position adjacent to the top end (as shown in Figure 4 and the bottom end position (as shown in Figure 6 ). However, in other variations of this embodiment, the relative positions of the guiding member 522 and the output shaft 532 on the first axial direction L1 can also be adjusted to change the instantaneous speed relationship between different positions, so it is not limited to this.

[0069] Step Four: The homogenized biological sample S together with the separated nucleic acid will adhere to the magnetic beads 212. At this time, as shown in Figure 9 , another variable-speed lifting unit 5 drives the magnetic bead transfer unit 7, drives each magnetic rod 72 to pass through its respective stirring sleeve 221, and makes the magnetic part 722 of each magnetic rod 72 adjacent to the bottom end of its respective stirring sleeve 221, thereby adsorbing the magnetic beads 212 on the outer side of the stirring sleeve 221. Then, the variable-speed lifting unit 5 synchronously drives the stirring sleeve 221 and the magnetic rod 72 to rise and move away from the reagent cartridge 21. It should be noted that in this embodiment, two variable-speed lifting units 5 with the same structure are provided to make the stirring sleeve 221 and the magnetic rod 72 rise at the same speed. However, in other variations, the variable-speed lifting unit 5 that drives the magnetic rod 72 can also be replaced with other driving methods such as belts, screws, or linkage mechanisms, and it cannot be limited to this.

[0070] Step Five: After the reagent cartridge transfer unit 4 drives the reagent cartridge 21 to be transferred along the second axial direction L2 and makes another reagent tank 211 of the reagent cartridge 21 located below the stirring sleeve 221, then repeat the stirring and transfer procedures of Step Three and Step Four. At this time, the reagent 213 for removing other non-nucleic acid substances is used and repeatedly washed in different reagent tanks 211 to achieve the purpose of purification and extraction. In addition, in this embodiment, the heating module 44 can also be used to heat during specific biochemical reactions, thereby improving the reaction efficiency.

[0071] Step Six: The reagent 213 contained in the last reagent tank 211 is used to precipitate the nucleic acid of the biological sample S from the magnetic beads 212. Then, drive the other variable-speed lifting unit 5 to drive the magnetic rod 72 away from the reagent cartridge 21, and it is available for the operator to take out the reagent cartridge 21 that has completed the biochemical reaction from this embodiment.

[0072] Step 7: After the operator then takes out the stirring and discharging part 22, start the disinfection unit 8, and perform ultraviolet disinfection on the reagent cassette transfer unit 4 and the magnetic bead transfer unit 7 to further reduce the generation of contamination.

[0073] The test results of this embodiment are summarized in Tables 1 and 2 below. Among them, the existing extraction device is as described in the background art, in the way that the screw drives the stirring sleeve to move up and down at a constant speed. The test results are to perform nucleic acid extraction experiments twice on two biological samples S under the same extraction reagent, the same number of vibrations, and the same vibration amplitude, and take the average value of the nucleic acid concentration (unit: ng / μl) for comparison of the nucleic acid extraction recovery rate; in addition, taking the recovery rate of the existing extraction device as 100%, calculate the recovery rate of this embodiment. The absorbance ratio (OD260 / OD280 ratio) represents the purity of the extracted nucleic acid. When the absorbance ratio is greater than 1.6, it means that a better extraction purity has been achieved.

[0074] Table 1. Test with 200 μl of human blood:

[0075]

[0076] Table 2. Test with 40 mg of chicken spleen tissue:

[0077]

[0078] With the above structure and operation procedure, this embodiment has the following advantages:

[0079] 1. When separating and destroying the cell wall and cell membrane of the biological sample S in the reagent 213 with the function of cell tissue lysis, by driving the stirring sleeve 221 to move at a variable speed by one of the variable-speed lifting units 5, the magnetic beads 212 placed in the reagent tank 211 can be stirred more powerfully, achieving a better grinding effect, and thus increasing the proportion of released nucleic acid.

[0080] 2. After the biological sample S is homogenously lysed, it still needs to be further washed repeatedly to remove other non-nucleic acid substances. By driving the stirring sleeve 221 to move at a variable speed, the turbulence intensity in the reagent 213 will be increased, further achieving an excellent mixing and stirring effect. In addition to improving the stirring uniformity, it is also beneficial to the transfer of heat energy and shortening the stirring time.

[0081] III. By means of the variable speed transmission module 52, in addition to reducing the overall volume and weight compared with the prior art to achieve better portability, a better mixing and stirring effect can also be achieved. Therefore, the programming requirements for the rotation module 53 can be simplified, and thus there is no need to purchase a high-price controller required for complex programming, further reducing the overall cost required, which is more conducive to portability.

[0082] IV. Compared with the high power consumption characteristics of existing biochemical reactors, in this embodiment, by means of the variable speed transmission module 52, the output shaft 532 of the rotation module 53 can rotate a very small number of turns to achieve the purpose of stirring, and the power consumption will be greatly reduced in use. This will facilitate the feasibility of battery-powered operation (without plugging in), and thus it is suitable to apply this embodiment in the first-line scenarios outside the laboratory to achieve better convenience and popularization.

[0083] In summary, the purpose of the present invention can indeed be achieved.

[0084] However, what is described above is only an embodiment of the present invention, and the scope of implementation of the present invention cannot be limited thereby. All simple equivalent changes and modifications made according to the claims and the content of the specification of the present invention still fall within the scope covered by the present invention.

Claims

1. A portable biochemical reactor, applicable to driving at least one stirring sleeve to displace relative to at least one reagent cartridge along a first axial direction, the portable biochemical reactor comprising a frame unit, characterized in that: The portable biochemical reactor further comprises at least one variable-speed lifting unit; The frame unit is applicable to supporting the at least one reagent cartridge; And The at least one variable-speed lifting unit includes: A linear movement module, having a guide rail connected to the frame unit, and a slider movably connected to the guide rail along the first axial direction and applicable to mounting the at least one stirring sleeve, the slider moving relative to the frame unit between a top position and a bottom position, at the top position, the slider being applicable to moving away from the at least one reagent cartridge, at the bottom position, the slider being applicable to approaching the at least one reagent cartridge, A variable-speed transmission module, having a transmission member and a guiding member, the transmission member having a pivot portion and a guiding portion arranged in reverse, the guiding member movably connected to the guiding portion, the guiding portion of the transmission member being a long slot, the guiding member sliding within the guiding portion, one of the guiding member and the pivot portion of the transmission member being connected to the slider, the slider being connected to the guiding member, and A rotation module, connected to the other of the guiding member and the pivot portion of the transmission member, and for driving the transmission member to rotate, such that during the rotation of the transmission member, the slider is driven by the guiding member to displace along the first axial direction following the guide rail, and synchronously drives the at least one stirring sleeve to perform a variable-speed linear movement, defining a second axial direction and a third axial direction that are orthogonal to the first axial direction and orthogonal to each other, the rotation module and the guiding member being located on two opposite sides of the guide rail along the third axial direction, and having a power member and an output shaft, the power member being connected to the frame unit, the output shaft being pivotally arranged on the power member along the second axial direction, and relative to the linear movement module being connected to the other of the guiding member and the pivot portion of the transmission member, the pivot portion of the transmission member being fixed to the rotation module; The at least one reagent cartridge is filled with a reagent and a liquid level is defined, the slider can further move relative to the frame unit to a midpoint position between the top position and the bottom position, at the midpoint position, the slider being applicable to driving the bottom end of the at least one stirring sleeve to approach the liquid level, and the instantaneous speed of the slider moving to the midpoint position is slower than the instantaneous speed of moving to adjacent the top position.

2. The portable biochemical reactor according to claim 1, wherein: The portable biochemical reactor further comprises a reagent cartridge transfer unit, and a second axial direction orthogonal to the first axial direction is defined, the reagent cartridge transfer unit includes two transfer tracks arranged on the frame unit along the second axial direction, a transfer seat movably arranged on the transfer tracks, and a driving module for driving the transfer seat to move relative to the frame unit.

3. The portable biochemical reactor according to claim 2, wherein: The driving module has a rack mounted on the transfer seat, a gear pivotally arranged on the frame unit, and a driving member for driving the gear.

4. The portable biochemical reactor according to claim 2, wherein: The rack unit includes a chassis and side frames. The side frames are disposed on the chassis and have openings. The transfer seat of the reagent cassette transfer unit has a seat body portion disposed on the transfer track and a jig portion removably connected to the seat body portion. The jig portion is used for mounting the at least one reagent cassette and can be taken out and put into through the opening of the side frame.

5. The portable biochemical reactor according to claim 2, wherein: The reagent cassette transfer unit further includes a heating module. The transfer seat has a seat body portion and a jig portion. The seat body portion is disposed on the transfer track and is provided for mounting the heating module. The jig portion is removably connected to the seat body portion and is used for mounting the at least one reagent cassette and has a through hole corresponding to the heating module.

6. The portable biochemical reactor according to claim 1, wherein: The portable biochemical reactor further includes a magnetic bead transfer unit. The portable biochemical reactor includes two variable-speed lifting units. The at least one reagent cassette is adapted to be filled with a plurality of magnetic beads. A slider of one of the variable-speed lifting units is connected to the stirring sleeve. Another variable-speed lifting unit is connected to the magnetic bead transfer unit. The magnetic bead transfer unit includes at least one magnetic rod inserted into the at least one stirring sleeve. The at least one magnetic rod has a magnetic portion adjacent to the bottom of the stirring sleeve. When the slider is in the bottom position, the magnetic portion is adapted to magnetically adsorb the magnetic beads on the stirring sleeve.

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