A biomechanical loading device based on hard materials
By designing a biomechanical loading device on hard materials and applying mechanical stimulation using the motor drive system, the problem that traditional research cannot simulate the environment of high-stiff bone tissue is solved, and a more realistic exploration of the biological functions of bone cells is achieved, and bones are promoted. Advances in integrated research.
Patent Information
- Application Number
- CN202110489036.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-05-06
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2041-05-06
AI Technical Summary
Traditional biomechanical research is mainly carried out on flexible basement membranes, and it is impossible to effectively simulate the mechanical environment of bone tissue with high stiffness, which limits the exploration of the biomechanical response mechanism of osteocytes.
Design a biomechanical loading device based on hard materials, including controllers, drive mechanisms and actuators, using motor drive chips, encoders and Maxon DC DC motors to simulate the mechanical environment of bone tissue through wire ropes and lever systems, and apply parameters Controllable mechanical stimulation.
Mechanical loading on hard materials effectively overcomes the limitations of traditional research, simulates the real bone tissue mechanical environment, helps explore the changes in the biological function of cells in high stiffness environments, and provides new ideas for bone integration research.
Smart Images
Figure CN113150988B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to a biomechanical loading device based on hard materials. Background Art
[0002] Bone tissue is a mechanical load-bearing organ that supports the human body, so bone tissue is always in a complex biomechanical environment. Osteoblasts, chondrocytes and other bone cells have the ability to respond to biomechanical stimuli. Biomechanical signals in daily life can regulate the biological functions of various types of bone cells. Lack of effective mechanical stimulation can lead to bone metabolism imbalance. For example, astronauts who have been in a weightless environment in space for a long time will experience significant bone loss in their load-bearing bones. This shows that maintaining mechanical stimulation plays an important role in bone balance. In order to better explore the relationship between mechanical stimulation and bone tissue, it is necessary to clarify the biomechanical response mechanism of bone tissue and bone cells. However, most traditional biomechanical studies use the Flexcell system, which is completed by inoculating cells on a flexible basement membrane and applying tensile stress or compressive stress to the basement membrane. This research model cannot well simulate the mechanical environment of bone tissue with high stiffness, thereby limiting the promotion significance of its research results.
[0003] Bone tissue is different from other human organs. In order to adapt to the human body's load-bearing and movement functions, it has strong stiffness and high elastic modulus. Therefore, if you want to explore the mechanism of cells sensing biomechanical signals inside real bone tissue, you urgently need to build a device that can explore the biomechanical function of cells on hard materials. Summary of the invention
[0004] The purpose of the present invention is to overcome the defects of the prior art and provide a biomechanical loading device based on hard materials. By using specific metal or alloy materials, the biomechanical properties of cells on the surface of implants can be explored, and the high-rigidity bone tissue mechanical environment in the body can be well simulated.
[0005] The technical solution to achieve the above object is: a biomechanical loading device based on hard materials, including a controller, a driving mechanism and an actuator, wherein the driving mechanism and the actuator are arranged on a substrate, wherein:
[0006] The actuator includes a push-down lever and a force-adding auxiliary container; the push-down lever includes a lever, a connecting frame, a supporting seat and a bracket, the lever is arranged horizontally, one end of the lever is inserted into and fixed in the connecting frame, and the other end of the lever is provided with a fixing point on the wire rope; the supporting seat, the force-adding auxiliary container and the bracket are arranged on the base plate from left to right in sequence; two connecting heads are arranged at the bottom end of the connecting frame, one of which is connected to the supporting seat through a rotating shaft, and the other connecting head is in contact with the top end of the force-adding auxiliary container; the lever is overlapped on the bracket;
[0007] The driving mechanism includes a motor driving chip, a motor encoder and a motor; the motor driving chip and the motor are respectively arranged on the substrate and on both sides of the bracket, and the motor encoder communicates with the motor driving chip and the motor respectively; the output shaft of the motor is a fixed point under the wire rope;
[0008] A steel wire rope is arranged between the upper fixing point of the steel wire rope and the lower fixing point of the steel wire rope;
[0009] The controller communicates with the motor drive chip.
[0010] The above-mentioned biomechanical loading device based on hard materials, wherein the controller adopts Elmo motion control software; the motor driver chip adopts Gold Twitter servo driver chip; the model of the motor encoder is AEDL 5810; and the motor adopts Maxon DC motor, model RE40.
[0011] In the above-mentioned biomechanical loading device based on hard materials, a motor encoder interface is provided on the motor driving chip, one end of the motor encoder is connected to the motor encoder interface through a communication line, and the other end is connected to the motor.
[0012] The above-mentioned biomechanical loading device based on hard materials, wherein the controller is used to set the motor operating mechanical parameters; the motor driving chip reads the motor operating mechanical parameters set on the controller; and the motor encoder sends the operating mechanical parameter instructions read by the motor driving chip to the motor.
[0013] In the above-mentioned biomechanical loading device based on hard materials, the force-adding auxiliary container includes an upper pressure column, a column base and a shell, wherein:
[0014] The upper pressure column and the column base are arranged one above the other in the housing;
[0015] The upper pressure column includes a top plate and four upper columns evenly distributed on the lower surface of the top plate, the column base includes a bottom plate and four lower columns evenly distributed on the upper surface of the bottom plate, the four upper columns are plugged into the four lower columns one by one, and each upper column and its corresponding lower column form a telescopic column;
[0016] An upper card slot is arranged on the lower surface of the top plate, and a lower card slot is arranged on the upper surface of the bottom plate, wherein the upper card slot is located directly above the lower card slot.
[0017] The above-mentioned biomechanical loading device based on hard materials, wherein a force-assisting container mounting groove is provided on the base plate, and the bottom end of the shell is inserted into the force-assisting container mounting groove; the top plate of the upper pressure column is connected to the corresponding connector.
[0018] In the above-mentioned biomechanical loading device based on hard materials, the support seat and the connecting head connected to the rotating shaft are respectively provided with axial holes, and the rotating shaft is inserted into the axial holes on the support seat and the connecting head.
[0019] The biomechanical loading device based on hard materials of the present invention has the following advantages:
[0020] (1) Mechanical loading on hard materials to study cell biological functions effectively solves the limitations of traditional biomechanical loading studies on flexible basement membranes;
[0021] (2) The stiffness of hard materials is more consistent with the base environment of bone tissue in the human body, and biomechanical loading based on hard materials can better simulate the real biomechanical environment;
[0022] (3) The biomechanical research on the traditional flexible basement membrane is not suitable for the mechanical environment at the implant-host bone interface. The biomechanical loading device of the present invention can better explore the biological function changes of cells after the adhesion and growth of cells on the implant surface and the indirect mechanical action of the implant. The results of this biomechanical research can provide help for a more realistic exploration of the biomechanical response mechanism of bone tissue, which is conducive to the exploration of the bone integration of implants and host bones, and provide new research ideas for clinical bone integration research. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a three-dimensional structural diagram (front view direction) of the biomechanical loading device based on hard materials of the present invention;
[0024] Figure 2 It is a three-dimensional structural diagram (backward viewing direction) of the biomechanical loading device based on hard materials of the present invention;
[0025] Figure 3 It is a structural schematic diagram of the booster auxiliary container;
[0026] Figure 4 This is the exploded structure diagram of the booster auxiliary container. DETAILED DESCRIPTION
[0027] In order to enable those skilled in the art to better understand the technical solution of the present invention, the specific implementation methods thereof are described in detail below in conjunction with the accompanying drawings:
[0028] See also Figure 1 , Figure 2 , Figure 3 and Figure 4 The best embodiment of the present invention is a biomechanical loading device based on hard materials, comprising a controller (not shown), a driving mechanism and an actuator, wherein the driving mechanism and the actuator are arranged on a substrate 1, wherein:
[0029] The actuator includes a push-down lever 2 and a force-adding auxiliary container 3; the push-down lever 2 includes a lever 21, a connection frame 22, a support seat 23 and a bracket 24, the lever 21 is arranged horizontally, one end of the lever 21 is inserted and fixed in the connection frame 22, and the other end of the lever 21 is provided with a fixed point 41 on the wire rope; the support seat 23, the force-adding auxiliary container 3 and the bracket 24 are arranged on the base plate 1 from left to right in sequence; two connectors 25 and 26 are arranged at the bottom end of the connection frame 22, one of the connectors 25 is connected to the support seat 23 through a rotating shaft 27, and the other connector 26 is in contact with the top of the force-adding auxiliary container 3; the lever 21 is overlapped on the bracket 24. Specifically, the support seat 23 and the connector 25 are respectively provided with shaft holes, and the rotating shaft 27 is inserted into the shaft holes on the support seat 23 and the connector 25.
[0030] Please see again Figure 3 and Figure 4 The force-added auxiliary container 3 includes an upper pressure column 31, a column base 32 and an outer shell 33. The upper pressure column 31 and the column base 32 are arranged one above the other in the outer shell 33. The inner diameter of the outer shell is 4.3 cm, the outer diameter is 4.5 cm, and the height is 4 cm; the upper pressure column 31 includes a top plate 311 and four upper columns 312 evenly distributed on the lower surface of the top plate 311, the column base 32 includes a bottom plate 321 and four lower columns 322 evenly distributed on the upper surface of the bottom plate 321, the four upper columns 312 are plugged into the four lower columns 322 one by one, and each upper column 312 and its corresponding lower column 322 form a telescopic column; an upper card slot 313 is provided on the lower surface of the top plate 311, and a lower card slot 323 is provided on the upper surface of the bottom plate 321, and the upper card slot 313 is located directly above the lower card slot 323.
[0031] The base plate 1 is provided with a force-assisting container mounting groove 11 , and the bottom end of the housing 33 is inserted into the force-assisting container mounting groove 11 ; the top plate 311 of the upper pressure column 31 is connected to the connector 25 .
[0032] Please see again Figure 1 When the connecting frame 22 rotates around the rotation axis 27, the lever 21 is driven to rotate around the rotation axis 27. When the lever 21 is lifted upward, the upper pressure column 31 of the auxiliary container 3 is pulled upward, and the upper column 312 moves upward along the corresponding lower column 322; when the lever 21 is lifted downward, the upper pressure column 31 of the auxiliary container 3 is pressed downward; the upper column 312 moves downward along the corresponding lower column 322. The rotation axis 27 constitutes the support rotation point of the lever 21.
[0033] The driving mechanism includes a motor driving chip 5, a motor encoder (not shown) and a motor 6; the motor driving chip 5 and the motor 6 are respectively arranged on the substrate 1 and on both sides of the bracket 24, the motor encoder communicates with the motor driving chip 5 and the motor 6 respectively, the motor driving chip 5 is provided with a motor encoder interface 51, one end of the motor encoder is connected to the motor encoder interface 51 through a communication line, and the other end is connected to the motor 6. The controller communicates with the motor driving chip 5.
[0034] The output shaft of the motor 6 is the lower fixed point 42 of the steel wire rope. A steel wire rope is arranged between the upper fixed point 41 of the steel wire rope and the lower fixed point 42 of the steel wire rope. After the steel wire rope is tightened, the motor 6 rotates to pull down the lever 21 to exert a mechanical effect.
[0035] The controller is used to set the motor running mechanical parameters; the motor driving chip 5 reads the motor running mechanical parameters set on the controller; the motor encoder sends the running mechanical parameter instructions read by the motor driving chip 5 to the motor 6.
[0036] The controller uses Elmo motion control software; it is connected to the motor driver chip 5 via a USB cable, and the motor operation mechanical parameters such as motor speed, frequency, etc. are set in the software operation interface.
[0037] The motor driver chip 5 adopts the Gold Twitter servo driver chip; the motor driver chip has the advantages of ultra-high power, small size, light weight, etc. The main parameters are as follows: maximum power 5.2kW, rated voltage 70V, efficiency above 99%.
[0038] The model of the motor encoder is AEDL 5810; the main parameters are as follows: the number of channels is 3, the number of pulses per rotation is 5000, the maximum speed is 30000rpm, the voltage is 5.0V±10.0%, and the drive logic is EIA RS 422.
[0039] Motor 6 uses a Maxon DC motor, model RE40. The main parameters are as follows: speed constant 317rpm / V, speed / torque slope 3.14rpm / mNm, voltage 24V, no-load speed 7580rpm, no-load current 137mA, normal speed 6940rpm, torque 177mNm, current 6A, maximum efficiency 91%, torque constant 30.2mNm / A, bearing type: ball bearing, maximum allowable speed 12000rpm, weight 480g.
[0040] The biomechanical loading device based on hard materials of the present invention is used to inoculate cells on the hard material 100. Before mechanical loading, the culture dish loaded with the hard material is taken out from the constant temperature incubator, the culture dish cover is opened on the clean bench, the sterilized force-added auxiliary container storage sterilization box is opened, sterile gloves are put on the clean bench, a pair of sterile tweezers are taken out from the sterilization box, the hard material 100 in the culture dish is clamped with the tweezers, the upper pressure column 31 and the column base 32 are taken out from the sterilization box with the left hand, the lower end of the hard material 100 is first inserted into the lower groove 323 of the column base 32, and then the four upper columns 312 of the upper pressure column 31 are buckled into the four lower columns 322 of the column base 32 one by one, and the upper end of the hard material 100 is inserted into the upper groove 313 of the upper pressure column 31, and the culture medium is sucked with a pipette and added to the force-added auxiliary container to ensure that the cells have nutritional support during the force-added process. Finally, the upper part of the force-assisted container is sealed with a surgical incision sterile protective film, and it is placed in the force-assisted container mounting groove 11 on the substrate 1. The motor 6 is plugged into the power supply, the motor driver chip 5 is connected to the controller on the computer via USB, the Elmo motion control software is opened, the mechanical loading device is connected, and the motor operation mechanical parameters are set. The motor driver chip 5 reads the motor operation mechanical parameters set on the controller; the motor encoder sends the operation mechanical parameter instructions read by the motor driver chip 5 to the motor 6, the motor 6 reads the motor operation mechanical parameters, tightens the wire rope, and by operating the start button on the Elmo motion control software, after tightening the wire rope, the motor 6 rotates to pull down the lever 21 to apply mechanical action, and the connector 26 is pressed on the top of the force-assisted container 3 to start mechanical loading on the hard material 100. After the mechanical loading is fixed for a fixed time, click the stop button first to cancel the power of the wire rope. At this time, the wire rope is in a relaxed state, lift the lever 21, and take out the force-assisted container. Open the sterile protective film on the clean bench, open the lid of the force-assisted container 3 storage and disinfection box, put on sterile gloves, gently take out the upper pressure column 31 and the column base 32 combination, remove the upper pressure column 31 with the right hand and put it into the force-assisted container storage and disinfection box, use sterile tweezers to remove the hard material 100 in the lower slot 323 of the column base 32, put it back to the original culture dish, remove the next hard material and repeat the above operation for mechanical loading.
[0041] In this way, the real biomechanical environment can be better simulated; mechanical loading can be performed on hard materials to study cell biological functions, which effectively solves the limitations of biomechanical loading research on traditional flexible basement membranes; the biological function changes of cells after adhesion and growth on the implant surface and indirect mechanical effects of the implant can be better explored. The results of this biomechanical study can provide assistance for a more realistic exploration of the biomechanical response mechanism of bone tissue, which is beneficial to the exploration of implant-host bone bone integration and provide new research ideas for clinical bone integration research.
[0042] The basic principle of the hard material-based biomechanical loading device of the present invention is to indirectly explore the biological function changes of cells adhering to and growing on the surface of the hard material by applying mechanical stimulation with controllable parameters to the hard material, thereby achieving the purpose of simulating the biomechanical environment in vivo. The biomechanical loading device of the present invention can be used to explore the biomechanical properties of cells on the surface of plants by using specific metal or alloy materials.
[0043] In summary, the hard material-based biomechanical loading device of the present invention can realize the exploration of the biomechanical properties of cells on the surface of implants by using specific metal or alloy materials, and can well simulate the high-rigidity bone tissue mechanical environment in vivo.
[0044] Those skilled in the art should recognize that the above embodiments are only used to illustrate the present invention, and are not intended to limit the present invention. As long as they are within the spirit of the present invention, any changes or modifications to the above embodiments will fall within the scope of the claims of the present invention.
Claims
1. A biomechanical loading device based on hard materials, characterized in that: It includes a controller, a driving mechanism and an actuator, wherein the driving mechanism and the actuator are arranged on a substrate, wherein: The actuator includes a push-down lever and a force-adding auxiliary container; the push-down lever includes a lever, a connecting frame, a supporting seat and a bracket, the lever is arranged horizontally, one end of the lever is inserted into and fixed in the connecting frame, and the other end of the lever is provided with a fixing point on the wire rope; the supporting seat, the force-adding auxiliary container and the bracket are arranged on the base plate from left to right in sequence; two connecting heads are arranged at the bottom end of the connecting frame, one of which is connected to the supporting seat through a rotating shaft, and the other connecting head is in contact with the top end of the force-adding auxiliary container; the lever is overlapped on the bracket; The driving mechanism includes a motor driving chip, a motor encoder and a motor; the motor driving chip and the motor are respectively arranged on the substrate and on both sides of the bracket, and the motor encoder communicates with the motor driving chip and the motor respectively; the output shaft of the motor is a fixed point under the wire rope; A steel wire rope is arranged between the upper fixing point of the steel wire rope and the lower fixing point of the steel wire rope; The controller communicates with the motor drive chip; The force-assisted container comprises an upper pressure column, a column base and a shell, wherein: The upper pressure column and the column base are arranged one above the other in the housing; The upper pressure column includes a top plate and four upper columns evenly distributed on the lower surface of the top plate, the column base includes a bottom plate and four lower columns evenly distributed on the upper surface of the bottom plate, the four upper columns are plugged into the four lower columns one by one, and each upper column and its corresponding lower column form a telescopic column; An upper card slot is disposed on the lower surface of the top plate, and a lower card slot is disposed on the upper surface of the bottom plate, wherein the upper card slot is located directly above the lower card slot; The base plate is provided with a force-assisted container mounting groove, and the bottom end of the shell is inserted into the force-assisted container mounting groove; the top plate of the upper pressure column is connected to the corresponding connector; The support seat and the connector connected to the rotating shaft are respectively provided with shaft holes, and the rotating shaft is inserted into the shaft holes on the support seat and the connector.
2. A biomechanical loading device based on hard materials according to claim 1, characterized in that: The controller adopts Elmo motion control software; the motor driver chip adopts Gold Twitter servo driver chip; the motor encoder model is AEDL 5810; the motor adopts Maxon DC motor, model RE40.
3. A biomechanical loading device based on hard materials according to claim 1, characterized in that: The motor drive chip is provided with a motor encoder interface, one end of the motor encoder is connected to the motor encoder interface via a communication line, and the other end is connected to the motor.
4. A biomechanical loading device based on hard materials according to claim 1, characterized in that: The controller is used to set the motor running mechanical parameters; the motor driving chip reads the motor running mechanical parameters set on the controller; the motor encoder sends the running mechanical parameter instructions read by the motor driving chip to the motor.