Peptide modified chitosan hydrogel composition and preparation method thereof

A composition, chitosan technology, applied in the field of repair, can solve the problems of slow repair, lack of support and assistance in cell production, weak repair function of hydrogel cell damage, etc., to achieve the effect of improving vitality

CN113304308AInactive Publication Date: 2021-08-27美卓(杭州)医疗科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2021-08-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a peptide modified chitosan hydrogel composition which is prepared from the following raw materials in parts by weight: 8.5 to 9.2 parts of sodium hydroxide, 10.3 to 11.4 parts of a cross-linking agent, 0.2 to 0.35 part of an initiator, 17.4 to 19.6 parts of acrylic acid, 1.2 to 1.6 parts of chitosan powder, 10 to 20 parts of a bioactive component, 5 to 8 parts of a nutrient substance and the balance of water. According to the invention, the hydrogel is prepared by using chitosan, so that the prepared hydrogel has better biodegradability, cell affinity and biological effect, the growth of cells can be better assisted, and the activity of cell repair can be further improved by adding peptide modified components, the bioactive components can improve the vitality of cell repair, and the nutrient substances can provide a part of energy for cell repair, so that cell repair is comprehensively assisted.
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Description

technical field

[0001] The invention belongs to the technical field of restoration, and in particular relates to a peptide-modified chitosan hydrogel composition and a preparation method thereof. Background technique

[0002] Chitosan (chitosan) is the product of N-deacetylation of chitin. Chitin, chitosan, and cellulose have similar chemical structures. Chitin and chitosan have many unique properties such as biodegradability, cell affinity and biological effects, especially chitosan containing free amino groups, which is one of the natural polysaccharides The only alkaline polysaccharide.

[0003] Hydrogel is a kind of extremely hydrophilic three-dimensional network structure gel, which swells rapidly in water and can maintain a large volume of water without dissolving in this swollen state. Due to the existence of a cross-linked network, the hydrogel can swell And retain a large amount of water, the water absorption is closely related to the degree of crosslinking. The ...

Examples

preparation example Construction

[0033] The preparation method of the composition of the chitosan hydrogel of described peptide modification comprises the steps:

[0034] Step S1, first increase the temperature of water to 20-30°C, keep the water temperature, add sodium hydroxide to stir, stir until the sodium hydroxide is completely dissolved in water, add acrylic acid to carry out stirring pretreatment, keep at constant temperature for 15 minutes, and obtain the first reaction solution;

[0035] Step S2, dissolving chitosan powder in the first reaction solution and stirring, heating the solution to 65° C., maintaining the stirring operation and passing nitrogen gas for 20 minutes, to obtain the second reaction solution;

[0036] Step S3, cooling the second reaction solution to 20-30° C., adding biologically active components and nutrients and stirring for 10-20 minutes to obtain a third reaction solution;

[0037] Step S4, adding an initiator to the third reaction solution, keeping the solution temperature...

Embodiment 1

[0055] A peptide-modified chitosan hydrogel composition prepared from the following raw materials in parts by weight: 8.6 parts of sodium hydroxide, 10.8 parts of a crosslinking agent, 0.28 parts of an initiator, 17.5 parts of acrylic acid, and 1.4 parts of chitosan powder 12 parts, 12 parts of bioactive components, 6 parts of nutrients and the balance of water;

[0056] The preparation method of the composition of the chitosan hydrogel of described peptide modification comprises the steps:

[0057] Step S1, first increase the temperature of the water to 20-30°C, keep the water temperature, then add sodium hydroxide for stirring, stir until the sodium hydroxide is completely dissolved in water, add acrylic acid for stirring pretreatment, and let stand at constant temperature for 15 minutes to obtain the first reaction solution;

[0058] Step S2, dissolving the chitosan powder in the first reaction solution and stirring, increasing the temperature of the solution to 65° C., ma...

Embodiment 2

[0064] A peptide-modified chitosan hydrogel composition prepared from the following raw materials in parts by weight: 8.8 parts of sodium hydroxide, 11.2 parts of a crosslinking agent, 0.34 parts of an initiator, 19.5 parts of acrylic acid, and 1.5 parts of chitosan powder 18 parts, 18 parts of bioactive components, 7.5 parts of nutrients and water balance;

[0065] The preparation method of the composition of the chitosan hydrogel of described peptide modification comprises the steps:

[0066] Step S1, first increase the temperature of the water to 20-30°C, keep the water temperature, then add sodium hydroxide for stirring, stir until the sodium hydroxide is completely dissolved in water, add acrylic acid for stirring pretreatment, and let stand at constant temperature for 15 minutes to obtain the first reaction solution;

[0067] Step S2, dissolving the chitosan powder in the first reaction solution and stirring, increasing the temperature of the solution to 65° C., maintai...